CN111208190B - Sampling head, sampling system, mass spectrum imaging device and sampling method - Google Patents
Sampling head, sampling system, mass spectrum imaging device and sampling method Download PDFInfo
- Publication number
- CN111208190B CN111208190B CN202010027705.8A CN202010027705A CN111208190B CN 111208190 B CN111208190 B CN 111208190B CN 202010027705 A CN202010027705 A CN 202010027705A CN 111208190 B CN111208190 B CN 111208190B
- Authority
- CN
- China
- Prior art keywords
- sampling
- flow channel
- cavity
- head
- port
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/26—Mass spectrometers or separator tubes
Abstract
The invention discloses a sampling head, a sampling system, a mass spectrum imaging device and a sampling method, wherein the sampling head comprises: sampling a body; the sampling cavity is arranged on the first end face of the sampling body, and the sampling cavity and the sampled surface form a sampling area; a first flow channel for injecting an extractant; a second flow channel for injecting air and a third flow channel for conducting out the sample droplets. The sampling head in the invention is directly contacted with the surface to be sampled, so that sampling can be realized without separation, the sampled surface is not wounded by adopting a liquid drop extraction mode, the sampling cavity is close to the sampled surface, the concentration of the liquid drop of the collected sample is improved, the possibility of information loss of key compounds is reduced, and the sampling quality is improved.
Description
Technical Field
The invention relates to the technical field of lipidomics, in particular to a sampling head, a sampling system, a mass spectrum imaging device and a sampling method.
Background
With the development of mass spectrometry technology, lipidomics have shown wide application prospects in the aspects of tumor biomarker identification, disease diagnosis, drug target and lead compound discovery, drug action mechanism research and the like. At present, metabolic molecular characteristics of tumor tissues are obtained by performing mass spectrometry on ex-vivo tissue surface substance samples, and the freezing and cutting of ex-vivo tissues can cause cell folding and breaking, so that information of partial key compounds is lost and the number of interfering compounds overflowing from cells is increased.
Therefore, how to improve the sampling quality is an urgent problem to be solved by those skilled in the art.
Disclosure of Invention
In view of the above, the technical problem to be solved by the present invention is how to improve the sampling quality, and therefore, the present invention provides a sampling head, a sampling system, a mass spectrometry imaging apparatus, and a sampling method.
In order to achieve the purpose, the invention provides the following technical scheme:
a sampling head, comprising:
sampling a body;
the sampling cavity is arranged on the first end face of the sampling body, and the sampling cavity and the sampled surface form a sampling area;
the sampling cavity is provided with a first end face and a second end face, the first end face is provided with a first flow channel, the first flow channel is used for injecting an extracting agent, the first flow channel is arranged on the sampling body, a first interface of the first flow channel is positioned on the second end face of the sampling body, and a second interface of the first flow channel is communicated with the sampling cavity;
the second flow channel is used for injecting air, the second flow channel is arranged on the sampling body, a first interface of the second flow channel is positioned on the second end face of the sampling body, and a second interface of the second flow channel is communicated with the sampling cavity; and
and the third flow channel is used for guiding out a sampling liquid drop, the third flow channel is arranged on the sampling body, a first interface of the third flow channel is positioned on the second end face of the sampling body, and a second interface of the third flow channel is communicated with the sampling cavity.
In one embodiment of the present invention, the second port of the first flow channel and the second port of the second flow channel are located at the top of the sampling cavity.
In one embodiment of the present invention, the aperture of the second interface of the first flow channel gradually increases along the injection direction of the extractant.
In one embodiment of the present invention, the second port of the first flow channel is concentric with the sampling cavity; the section of the second interface of the second flow passage is of a fan-shaped structure.
In one embodiment of the present invention, the second port of the second flow channel is in communication with the second port of the first flow channel, and is in communication with the sampling cavity through the second port of the first flow channel.
In one embodiment of the present invention, the second interface of the second flow channel is flush with the second interface of the first flow channel along the air injection direction.
In one embodiment of the present invention, the second port of the third flow channel is near the bottom of the sampling cavity.
In one embodiment of the present invention, the sampling body includes a first sampling body, a second sampling body, and a third sampling body, wherein the second sampling body is located in the middle of the first sampling body and the third sampling body.
In one embodiment of the present invention, the portion of the first flow channel located in the first sampling body, the portion of the second flow channel located in the first sampling body, and the portion of the third flow channel located in the first sampling body extend in an axial direction of the first sampling body.
In one embodiment of the present invention, the portion of the first flow channel located in the second sampling body, the portion of the second flow channel located in the second sampling body, and the portion of the third flow channel located in the second sampling body are inclined toward the sampling cavity.
In one embodiment of the present invention, the bending portion of the first flow channel, the bending portion of the second flow channel, and the bending portion of the third flow channel are connected in a smooth transition manner.
In one embodiment of the present invention, the sampling cavity is located in the third sampling body, and the outer surface of the sampling body has a circular arc structure.
In one embodiment of the present invention, the sampling cavity includes a first sampling cavity and a second sampling cavity concentrically arranged, the first sampling cavity is communicated with the second interface of the first flow channel, the second interface of the second flow channel and the second interface of the third flow channel, and the second sampling cavity corresponds to the surface of the sampled tissue.
In one embodiment of the present invention, the aperture of the second sampling cavity is larger than the aperture of the first sampling cavity.
In one embodiment of the present invention, a portion of the third flow channel located in the third sampling body is smoothly and transitionally connected with a portion of the third flow channel located in the second sampling body.
In one embodiment of the invention, the sampling head is processed by an injection molding or 3D printing process.
The invention also discloses a sampling system, which comprises the sampling head.
The invention also discloses a mass spectrum imaging device which comprises the sampling system.
The invention also discloses a sampling method, which applies any one of the sampling heads, and comprises the following steps:
the second end surface of the sampling head is in parallel contact with the surface to be sampled and is kept still after being completely contacted;
injecting an extracting agent into the sampling cavity from the first flow channel, stopping injecting after the sampling cavity is full, and keeping the extracting agent in contact with the sampled surface for a certain time;
synchronously injecting air from the second flow channel and extracting air from the third flow channel, and conveying the liquid in the sampling cavity in the form of liquid drops through the third flow channel under the driving of air pressure;
after sampling is completed, the sampling head is lifted off the surface to be sampled.
According to the technical scheme, when the sampling head is used, the second end face of the sampling head is firstly in parallel contact with the surface to be sampled and is kept still after being completely contacted, and only air exists in the sampling cavity at the moment; secondly, injecting an extracting agent into the sampling cavity from the first flow channel, wherein the injection is stopped after the sampling cavity is filled with the extracting agent, and the extracting agent is contacted with the sampled surface in the process; then, the extractant is kept in contact with the sampled surface for a certain time, so that the extraction effect is fully generated; then, synchronously injecting air from the second flow channel and extracting air from the third flow channel, and conveying the liquid in the sampling cavity out through the third flow channel in a liquid drop mode under the driving of air pressure so as to enter next-stage analysis equipment; finally, the sampling is completed and the sampling head is lifted off the surface to be sampled. The sampling head in the invention is directly contacted with the surface to be sampled, so that sampling can be realized without separation, the sampled surface is not wounded by adopting a liquid drop extraction mode, the sampling cavity is close to the sampled surface, the concentration of the liquid drop of the collected sample is improved, the possibility of information loss of key compounds is reduced, and the sampling quality is improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
Fig. 1 is a schematic top perspective view of a sampling head according to an embodiment of the present invention;
fig. 2 is a schematic bottom perspective view of a sampling head according to an embodiment of the present invention;
fig. 3 is a schematic bottom perspective view of a sampling head according to an embodiment of the present invention;
FIG. 4 is an enlarged schematic view of portion A of FIG. 3;
fig. 5 is a schematic flow chart of a sampling method according to an embodiment of the present invention;
fig. 6 is a schematic flowchart of a sampling method according to an embodiment of the present invention;
in the figure, 100 is a sampling body, 101 is a first sampling body, 102 is a second sampling body, 103 is a third sampling body, 200 is a sampling cavity, 201 is a first sampling cavity, 202 is a second sampling cavity, 300 is a first flow channel, 301 is a first interface of the first flow channel, 302 is a second interface of the first flow channel, 400 is a second flow channel, 401 is a first interface of the second flow channel, 402 is a second interface of the second flow channel, 500 is a third flow channel, 501 is a first interface of the third flow channel, and 502 is a second interface of the third flow channel.
Detailed Description
Interpretation of terms:
mass spectrometry analysis: the method of detecting the moving ions after separating them by their mass-to-charge ratios using electric and magnetic fields allows the identification of the species by mass-to-charge ratio.
Mass spectrometry imaging technique: the mass spectrometry imaging is an imaging method based on mass spectrometry technology, and the method directly scans biological samples through mass spectrometry to image, and can simultaneously analyze the spatial distribution characteristics of hundreds of molecules on the same tissue slice or tissue chip. In brief, the mass spectrometry imaging technology is a method of imaging by using a mass spectrometer which analyzes the standard molecular weight of biomolecules by measuring mass-to-charge ratio under the control of special mass spectrometry imaging software.
And (3) extraction: the material is extracted using an extractant.
Surface sampling: a sample of the substance is taken from the sampled surface.
In vivo: the operation is directly carried out on the sampled surface without adopting an in vitro slicing mode.
A flow channel: fluid (gas/liquid) is in the path within the element.
The core of the invention is to provide a sampling head, a sampling system, a mass spectrum imaging device and a sampling method, so as to improve the sampling quality.
The embodiments described below do not limit the contents of the invention described in the claims. The entire contents of the configurations shown in the following embodiments are not limited to those required as solutions of the inventions described in the claims.
To this end, referring to fig. 1 to 4, the sampling head in the embodiment of the present invention includes:
sampling a body;
the sampling cavity 200 is arranged on the first end face of the sampling body, and the sampling cavity 200 and the sampled surface form a sampling area;
the first flow channel 300 is used for injecting an extracting agent, the first flow channel 300 is arranged on the sampling body, the first interface 301 of the first flow channel is positioned on the second end face of the sampling body, and the second interface 302 of the first flow channel is communicated with the sampling cavity 200;
the second flow channel 400 is used for injecting air, the second flow channel 400 is arranged on the sampling body, the first interface 401 of the second flow channel is positioned on the second end face of the sampling body, and the second interface 402 of the second flow channel is communicated with the sampling cavity 200; and
and the third flow channel 500 is used for leading out the sample liquid drop, the third flow channel 500 is arranged on the sample body, the first interface 501 of the third flow channel is positioned on the second end face of the sample body, and the second interface 502 of the third flow channel is communicated with the sampling cavity 200.
When the sampling head is used, the second end face of the sampling head is firstly in parallel contact with a surface to be sampled and is kept still after being completely contacted, and only air exists in the sampling cavity 200 at the moment; secondly, injecting the extracting agent into the sampling cavity 200 through the first flow channel 300, wherein the injection is stopped after the sampling cavity 200 is filled, and the extracting agent is contacted with the sampled surface in the process; then, the extractant is kept in contact with the sampled surface for a certain time, so that the extraction effect is fully generated; then, air is synchronously injected from the second flow channel 400 and extracted from the third flow channel 500, under the driving of air pressure, the liquid in the sampling cavity 200 is conveyed out through the third flow channel 500 in the form of liquid drops to enter the next-stage analysis equipment, and because the air synchronously flows in and out from the two flow channels, negative pressure cannot be formed in the sampling cavity, and the liquid level position of the extractant entering the first flow channel 300 is influenced; finally, the sampling is completed and the sampling head is lifted off the surface to be sampled. The sampling head in the invention is directly contacted with the surface to be sampled, so that sampling can be realized without separation, the sampled surface is not wounded by adopting a liquid drop extraction mode, the sampling cavity is close to the sampled surface, the concentration of the liquid drop of the collected sample is improved, the possibility of information loss of key compounds is reduced, and the sampling quality is improved.
It should be noted that, in the present invention, the sampling body 100 is a solid structure to form the first flow channel 300, the second flow channel 400, the third flow channel 500 and the supporting body of the sampling cavity 200, and may be made of resin, plastic or glass, and is processed by an injection molding process and a 3D printing technology, and when the 3D printing technology is adopted, the ultraviolet curing 3D printing technology is particularly adopted for processing.
The first interface 301 of the first flow channel is used for being externally connected with a pipeline for injecting an extracting agent, the first interface 401 of the second flow channel is used for being externally connected with a pipeline for injecting a positive pressure air source, and the first interface 501 of the third flow channel is used for being externally connected with a pipeline for forming a negative pressure outlet to lead out sampling liquid drops. The second port 302 of the first flow channel communicates with the sampling chamber, the second port 402 of the second flow channel communicates with the sampling chamber, and the second port 502 of the third flow channel communicates with the sampling chamber. The cross sections of the first flow channel 300, the second flow channel 400 and the third flow channel 500 are circular, oval, fan-shaped and the like.
Wherein, since the first flow channel 300 and the second flow channel 400 are respectively filled with the extractant and the air, the second interface 302 of the first flow channel and the second interface 402 of the second flow channel are located at the top of the sampling cavity 200 in order to facilitate the pressurization and the filling of the sampling cavity 200. Therefore, when the extractant is required to be injected, the extractant is gradually injected downwards from the top of the sampling cavity 200 through the second port 302 of the first flow channel, and after the extractant is filled and the extractant is fully contacted with the metabolite on the collected surface, the positive pressure gas source is gradually flushed from the top of the sampling cavity 200 through the second port 402 of the second flow channel, so that the sampling liquid drops in the sampling cavity 200 are pressed into the second port 502 of the third flow channel. In order to enable the high-concentration sampling liquid drops to firstly enter the second interface 502 of the third flow channel, so as to increase the concentration of the sampling liquid drops, and thus the detection detectable rate of subsequent detection can be increased, in the embodiment of the present invention, the second interface 502 of the third flow channel is close to the bottom of the sampling cavity 200.
The aperture of the second interface 302 of the first flow channel is gradually increased along the injection direction of the extractant, so that when the extractant enters the sampling cavity 200 through the first flow channel 300, the extractant firstly enters the sampling cavity 200 under the expansion effect of the second interface 302 of the first flow channel, the speed of the extractant entering the sampling cavity 200 can be gradually reduced, the generation of a turbulent flow phenomenon is reduced, and the phenomenon that the first flow channel 300 is blocked due to a surge phenomenon to influence the injection of the extractant is avoided. The second port 302 of the first flow channel may be concentric with the sampling cavity 200 or may be a non-concentric design.
The cross section of the second port 402 of the second flow passage is a fan-shaped structure. Because the air flow resistance is little and there is not remaining viscous scheduling problem, the second interface shape that second flow channel 400 inserts sampling cavity 200 is designed as the fan-shaped structure that two different concentric circular arcs of radius (minor arc) and the end point line enclose, and the sampling head structure is compacter like this.
The second port 402 of the second flow channel communicates directly with the sampling cavity 200, or the second port 402 of the second flow channel communicates with the second port 302 of the first flow channel and communicates with the sampling cavity 200 through the second port 302 of the first flow channel.
When the second interface 402 of the second flow channel is communicated with the second interface 302 of the first flow channel, the second interface 402 of the second flow channel is flush with the second interface 302 of the first flow channel along the air injection direction. That is, the inner circular arc of the second port 402 of the second flow passage as a fan-shaped structure is concentric with the minimum diameter of the second port 302 of the first flow passage; the outer arc of the second port 402 of the second flow channel is concentric with the largest diameter of the second port 302 of the first flow channel.
For convenience of description, in one embodiment of the present invention, the sampling body 100 includes a first sampling body 101, a second sampling body 102, and a third sampling body 103, wherein the second sampling body 102 is located in the middle of the first sampling body 101 and the third sampling body 103. The first sampling body 101, the second sampling body 102 and the third sampling body 103 are of an integrated structure or a split structure, and when the first sampling body, the second sampling body and the third sampling body are of a split structure, the first sampling body, the second sampling body and the third sampling body are connected through a bonding process.
For the sake of smoothness in injection of the extractant, the portion of the first flow channel 300 located in the first sampling body 101 extends in the axial direction of the first sampling body 101; for the sake of smoothness in air injection, the portion of the second flow channel 400 located in the first sample body 101 extends in the axial direction of the first sample body 101; to derive the smoothness of the sample droplet, the portion of the third flow channel 500 located in the first sample body 101 extends in the axial direction of the first sample body 101.
Further, the portion of first flow channel 300 located in second sampling body 102, the portion of second flow channel 400 located in second sampling body 102, and the portion of third flow channel 500 located in second sampling body 102 are angled toward sampling cavity 200. The angled arrangement enables the first flow channel 300, the second flow channel 400 and the third flow channel 500 to occupy less space on the portion of the second sampling body 102, thereby enabling the second sampling body 102 to have a smaller diameter than the first sampling body 101.
Because the first flow channel 300, the second flow channel 400 and the third flow channel 500 which are positioned on the second sampling body 102 are obliquely arranged, the first flow channel 300, the second flow channel 400 and the third flow channel 500 are all provided with bent parts, and in order to reduce the occurrence of the situation that the sample liquid drops are stuck or damaged due to the existence of the bent parts, the bent parts of the first flow channel 300, the bent parts of the second flow channel 400 and the bent parts of the third flow channel 500 are in smooth transition connection.
In order to further reduce the sampling area, in the embodiment of the present invention, the sampling cavity 200 is located in the third sampling body 103, and the outer surface of the sampling body 100 has a circular arc structure. This does not reduce the diameter of the third sampling body 103, thereby allowing a smaller effective sampling area, while the word sample droplet volume would be smaller, thereby allowing for improved sampling accuracy.
Further, the sampling cavity 200 is of a cylindrical structure or a hemispherical structure, the sampling cavity 200 is of a half-opening structure and is directly contacted with the sampled surface, and the second interface 502 of the third flow channel is closer to the sampled surface, so that the concentration of the sampling liquid drop is improved, and the possibility of information loss of key compounds can be reduced. The area of the sampling cavity 200 in direct contact with the sampled surface can reach millimeter level or even hundred micron level, and the smaller the area sampled by the sampling head, the higher the spatial resolution. The sampling cavity 200 comprises a first sampling cavity 201 and a second sampling cavity 202 which are concentrically arranged, the first sampling cavity 201 is communicated with a second interface 302 of the first flow channel, a second interface 402 of the second flow channel and a second interface 502 of the third flow channel, and the second sampling cavity 202 corresponds to the surface of the sampled tissue. In one embodiment of the present invention, the aperture of the second sampling cavity 202 is larger than the aperture of the first sampling cavity 201.
In order to increase the concentration of the sampling liquid drop, the second interface 502 of the third flow channel is close to the bottom of the sampling cavity 200, for this reason, a part of the third flow channel 500 is located in the third sampling body 103, and a part of the third flow channel 500 located in the third sampling body 103 is smoothly and transitionally connected with a part of the third flow channel 500 located in the second sampling body 102, so that the generation of a dead angle is avoided, and the sample loss and the pollution are reduced.
The invention also discloses a sampling system, which comprises the sampling head. Because the sampling head has the beneficial effects, a sampling system comprising the sampling head also has corresponding effects, which are not described again here.
The invention also discloses a mass spectrum imaging device which comprises the sampling system. Since the sampling system has the above beneficial effects, the mass spectrometry imaging device including the sampling system also has corresponding effects, which are not described herein again.
Referring to fig. 5 and fig. 6, the present invention further discloses a sampling method, which applies any one of the sampling heads, and the sampling method includes:
step S1: the second end surface of the sampling head is in parallel contact with the surface to be sampled and is kept still after being completely contacted;
step S2: injecting an extracting agent into the sampling cavity 200 through the first flow channel 300, stopping injecting after the sampling cavity 200 is full, and keeping the extracting agent in contact with the sampled surface for a certain time;
step S3: simultaneously injecting air from the second flow channel 400 and extracting air from the third flow channel 500, and conveying the liquid in the sampling cavity 200 in the form of liquid drops through the third flow channel 500 under the driving of air pressure;
step S4: after sampling is completed, the sampling head is lifted off the surface to be sampled.
In the description of the present application, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present application. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
Unless expressly stated or limited otherwise, the terms "mounted," "connected," and "connected" are intended to be inclusive and mean, for example, that they may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (19)
1. A sampling head, comprising:
sampling a body;
the sampling cavity is arranged on the first end face of the sampling body, and the sampling cavity and the sampled surface form a sampling area;
the sampling cavity is provided with a first end face and a second end face, the first end face is provided with a first flow channel, the first flow channel is used for injecting an extracting agent, the first flow channel is arranged on the sampling body, a first interface of the first flow channel is positioned on the second end face of the sampling body, and a second interface of the first flow channel is communicated with the sampling cavity;
the second flow channel is used for injecting air, the second flow channel is arranged on the sampling body, a first interface of the second flow channel is positioned on the second end face of the sampling body, and a second interface of the second flow channel is communicated with the sampling cavity; and
and the third flow channel is used for guiding out a sampling liquid drop, the third flow channel is arranged on the sampling body, a first interface of the third flow channel is positioned on the second end face of the sampling body, and a second interface of the third flow channel is communicated with the sampling cavity.
2. The sampling head of claim 1, wherein the second port of the first flow channel and the second port of the second flow channel are located at a top of the sampling cavity.
3. The sampling head of claim 2, wherein the second port of the first flow channel is progressively larger in diameter along the direction of extractant injection.
4. The sampling head of claim 3, wherein the second port of the first flow channel is concentric with the sampling cavity; the section of the second interface of the second flow passage is of a fan-shaped structure.
5. The sampling head of claim 4, wherein the second port of the second flow channel is in communication with the second port of the first flow channel and with the sampling cavity via the second port of the first flow channel.
6. The sampling head of claim 5, wherein the second port of the second flow passage is flush with the second port of the first flow passage in the direction of air injection.
7. The sampling head of claim 1, wherein the second port of the third flow channel is proximate a bottom of the sampling cavity.
8. The sampling head of any one of claims 1 to 6, wherein the sampling body comprises a first sampling body, a second sampling body, and a third sampling body, wherein the second sampling body is located intermediate the first sampling body and the third sampling body.
9. The sampling head of claim 8, wherein the portion of the first flow passage located in the first sampling body, the portion of the second flow passage located in the first sampling body, and the portion of the third flow passage located in the first sampling body extend in an axial direction of the first sampling body.
10. The sampling head of claim 9, wherein the portion of the first flow passage located in the second sampling body, the portion of the second flow passage located in the second sampling body, and the portion of the third flow passage located in the second sampling body are angled toward the sampling cavity.
11. The sampling head of claim 8, wherein the bend of the first flow channel, the bend of the second flow channel, and the bend of the third flow channel are in rounded transition.
12. The sampling head of claim 8, wherein the sampling cavity is located in the third sampling body, and an outer surface of the third sampling body is a rounded structure.
13. The sampling head of claim 12, wherein the sampling cavity comprises a first sampling cavity and a second sampling cavity concentrically arranged, the first sampling cavity in communication with the second port of the first flow channel, the second port of the second flow channel, and the second port of the third flow channel, the second sampling cavity corresponding to a surface of a tissue being sampled.
14. The sampling head of claim 13, wherein the aperture of the second sampling cavity is larger than the aperture of the first sampling cavity.
15. The sampling head of claim 13, wherein a portion of the third flow passage located in the third sampling body is rounded off from a portion of the third flow passage located in the second sampling body.
16. The sampling head of claim 1, wherein the sampling head is manufactured by an injection molding or 3D printing process.
17. A sampling system comprising a sampling head according to any one of claims 1 to 16.
18. A mass spectrometry imaging apparatus comprising the sampling system of claim 17.
19. A sampling method, wherein the sampling head according to any one of claims 1 to 16 is applied, the sampling method comprising:
the second end surface of the sampling head is in parallel contact with the surface to be sampled and is kept still after being completely contacted;
injecting an extracting agent into the sampling cavity from the first flow channel, stopping injecting after the sampling cavity is full, and keeping the extracting agent in contact with the sampled surface for a certain time;
synchronously injecting air from the second flow channel and extracting air from the third flow channel, and conveying the liquid in the sampling cavity in the form of liquid drops through the third flow channel under the driving of air pressure;
after sampling is completed, the sampling head is lifted off the surface to be sampled.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010027705.8A CN111208190B (en) | 2020-01-10 | 2020-01-10 | Sampling head, sampling system, mass spectrum imaging device and sampling method |
| PCT/CN2020/129153 WO2021139404A1 (en) | 2020-01-10 | 2020-11-16 | Sampling head, sampling system, mass spectrum imaging device and sampling method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010027705.8A CN111208190B (en) | 2020-01-10 | 2020-01-10 | Sampling head, sampling system, mass spectrum imaging device and sampling method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111208190A CN111208190A (en) | 2020-05-29 |
| CN111208190B true CN111208190B (en) | 2021-05-18 |
Family
ID=70785356
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010027705.8A Active CN111208190B (en) | 2020-01-10 | 2020-01-10 | Sampling head, sampling system, mass spectrum imaging device and sampling method |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN111208190B (en) |
| WO (1) | WO2021139404A1 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111199863B (en) * | 2020-01-10 | 2021-07-16 | 中国科学院深圳先进技术研究院 | Sampling system, mass spectrometry device, sampling method and mass spectrometry method |
| CN111208190B (en) * | 2020-01-10 | 2021-05-18 | 中国科学院深圳先进技术研究院 | Sampling head, sampling system, mass spectrum imaging device and sampling method |
| CN112768339B (en) * | 2020-12-07 | 2022-09-09 | 中国科学院深圳先进技术研究院 | Full-automatic micro-fluidic surface sampling system and method for mass spectrometry imaging |
| CN112768338B (en) * | 2020-12-07 | 2022-07-05 | 中国科学院深圳先进技术研究院 | Mass spectrometry high-spatial-resolution surface sampling head based on microfluidic technology and sampling method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102192948A (en) * | 2010-03-18 | 2011-09-21 | 复旦大学 | Method and device for sampling mass spectrum for in-situ nondestructive analysis |
| JP2011196697A (en) * | 2010-03-17 | 2011-10-06 | National Institute Of Advanced Industrial Science & Technology | Atomizer and plasma analyzer |
| CN102214591A (en) * | 2011-05-23 | 2011-10-12 | 叶伟清 | Method for sampling end surface of wafer |
| CN103038857A (en) * | 2010-05-07 | 2013-04-10 | Ut-巴特勒有限责任公司 | System and method for extracting a sample from a surface |
| CN109358189A (en) * | 2018-09-28 | 2019-02-19 | 湖南乐准智芯生物科技有限公司 | A kind of biochip and its micro quantitative determination sampling method |
| CN110085506A (en) * | 2018-01-26 | 2019-08-02 | 广州禾信仪器股份有限公司 | Sample introduction assembly, open type ion source system and mass spectrometer |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2467868A1 (en) * | 2009-08-17 | 2012-06-27 | Temple University Of The Commonwealth System Of Higher Education | Vaporization device and method for imaging mass spectrometry |
| CN102042992B (en) * | 2010-10-12 | 2013-06-05 | 红塔烟草(集团)有限责任公司 | Thermal analyzer for material pyrolysis behavior and flowing carrier gas-single-drop microextraction combined device and using method thereof |
| CN103226127B (en) * | 2013-03-27 | 2015-02-18 | 清华大学 | Multi-channel micro-fluidic chip and mass spectrum combined device |
| CN106248469B (en) * | 2016-10-31 | 2024-04-02 | 钟曼匀 | Liquid drop continuous micro-extraction device capable of automatically supplementing and collecting liquid drops and extraction method |
| GB2563121B (en) * | 2017-04-11 | 2021-09-15 | Micromass Ltd | Ambient ionisation source unit |
| CN111208190B (en) * | 2020-01-10 | 2021-05-18 | 中国科学院深圳先进技术研究院 | Sampling head, sampling system, mass spectrum imaging device and sampling method |
| CN111199863B (en) * | 2020-01-10 | 2021-07-16 | 中国科学院深圳先进技术研究院 | Sampling system, mass spectrometry device, sampling method and mass spectrometry method |
| CN111208191B (en) * | 2020-01-10 | 2021-05-18 | 中国科学院深圳先进技术研究院 | Sampling head, sampling system, mass spectrum imaging device and sampling method |
-
2020
- 2020-01-10 CN CN202010027705.8A patent/CN111208190B/en active Active
- 2020-11-16 WO PCT/CN2020/129153 patent/WO2021139404A1/en active Application Filing
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011196697A (en) * | 2010-03-17 | 2011-10-06 | National Institute Of Advanced Industrial Science & Technology | Atomizer and plasma analyzer |
| CN102192948A (en) * | 2010-03-18 | 2011-09-21 | 复旦大学 | Method and device for sampling mass spectrum for in-situ nondestructive analysis |
| CN103038857A (en) * | 2010-05-07 | 2013-04-10 | Ut-巴特勒有限责任公司 | System and method for extracting a sample from a surface |
| CN102214591A (en) * | 2011-05-23 | 2011-10-12 | 叶伟清 | Method for sampling end surface of wafer |
| CN110085506A (en) * | 2018-01-26 | 2019-08-02 | 广州禾信仪器股份有限公司 | Sample introduction assembly, open type ion source system and mass spectrometer |
| CN109358189A (en) * | 2018-09-28 | 2019-02-19 | 湖南乐准智芯生物科技有限公司 | A kind of biochip and its micro quantitative determination sampling method |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2021139404A1 (en) | 2021-07-15 |
| CN111208190A (en) | 2020-05-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111208190B (en) | Sampling head, sampling system, mass spectrum imaging device and sampling method | |
| CN111208191B (en) | Sampling head, sampling system, mass spectrum imaging device and sampling method | |
| CN111199863B (en) | Sampling system, mass spectrometry device, sampling method and mass spectrometry method | |
| US10943775B2 (en) | Collection probe and methods for the use thereof | |
| CN107446820B (en) | Single cell sampling and in-situ detection mass spectrum interface device based on micro-fluidic chip | |
| US8163257B2 (en) | Fluid dispenser | |
| CN108780063B (en) | Mass spectrometer and method for analyzing biological sample using same | |
| US9595428B2 (en) | Cellular probe device, system and analysis method | |
| EP0861431A1 (en) | Blood analysis system having blood storage, transport and automatic slide-making capabilities | |
| CN216484763U (en) | Test card structure of blood gas analyzer | |
| CN109357991A (en) | A kind of the mass spectrum fluidic cell sample introduction and ionization apparatus of label-free principle | |
| US10456777B2 (en) | Pressure transmission liquid for cellular analyzer, cellular analyzer and method for analyzing a liquid cellular sample | |
| CN105675379A (en) | Blood synchronously separating and detecting device | |
| WO2018045208A1 (en) | Collection probe and methods for the use thereof | |
| CN209822593U (en) | High-throughput single-cell electrospray mass spectrometry device | |
| US20240116052A1 (en) | Disposable separation chip | |
| CN105632866A (en) | Electro-spray ionization device and mass spectrometer | |
| CN111596134A (en) | Single cell bioelectricity parameter detection device and detection method based on compression channel | |
| CN217450211U (en) | Droplet generating device | |
| CN206321612U (en) | A kind of filter-type chromatogram bottle and chromatograph | |
| CN208459416U (en) | Micro-fluidic chip is loaded sealing system and the cytoanalyze comprising it | |
| CN117269287A (en) | Ink-jet sample injection-capillary electrophoresis-mass spectrum combined device and working method | |
| CN209108661U (en) | Continuous extraction apparatus | |
| CN113308533B (en) | Placenta exosome miRNA expression analysis method for gestational diabetes patient | |
| CN112768338B (en) | Mass spectrometry high-spatial-resolution surface sampling head based on microfluidic technology and sampling method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |