CN111205973A

CN111205973A - Adjustable bacteria incubator and use method thereof

Info

Publication number: CN111205973A
Application number: CN202010117886.3A
Authority: CN
Inventors: 胥振国; 范高福; 袁兴
Original assignee: Hefei Technology College
Current assignee: Hefei Technology College
Priority date: 2020-02-25
Filing date: 2020-02-25
Publication date: 2020-05-29
Anticipated expiration: 2040-02-25
Also published as: CN111205973B

Abstract

The invention belongs to the technical field of biology, and particularly relates to an adjustable bacteria incubator and a using method thereof. Through the pull ring of pulling backup pad one end, can remove the position of dish that disinfects, before the incubator uses, the inside of high temperature that the inside heating wire of dish that disinfects produced further transmits to the incubator through the heat conduction dish, thereby can disinfect for the inside radiation in center in the bottom of incubator, can pull the backup pad and the dish that disinfects to one side of working plate through further pulling pull ring, can be with the ninety degrees angle of backup pad rotation through the inside loose axle of mounting bracket, thereby it disinfects to carry out the heat radiation as the center to the incubator inside with the side of incubator, thereby be favorable to guaranteeing the sterile environment of incubator.

Description

Adjustable bacteria incubator and use method thereof

Technical Field

The invention belongs to the technical field of biology, and particularly relates to an adjustable bacterial incubator and a using method thereof.

Background

Bacterial culture is a technique in which bacteria are grown and propagated artificially. The bacteria are widely distributed in nature, large in quantity and multiple in types, can benefit mankind, and can also become a cause of diseases. Most bacteria can be cultured artificially by inoculating them on a culture medium and allowing them to grow and propagate. Bacterial culture is a complex technique. The culture method and medium should be selected according to the type and purpose of the bacteria, and the culture conditions should be established. The general operation steps are that the specimen is firstly inoculated on a solid culture medium for separation culture. The culture medium is usually prepared into liquid, semisolid, solid and the like by beef soup, peptone, sodium chloride, glucose, blood and the like and special substances required by certain bacteria. The general bacteria can grow in the presence of oxygen at 37 ℃ for 18-24 hours. Anaerobic bacteria need to grow in an anaerobic environment for 2-3 days. Individual bacteria such as tubercle bacillus are cultured for 1 month.

However, the existing bacteria culture device is inconvenient to use due to lack of high-temperature sterilization facilities, and the requirement for culturing bacteria is difficult to meet quickly.

Disclosure of Invention

To solve the problems set forth in the background art described above. The invention provides an adjustable bacteria incubator and a using method thereof, and solves the problem that a bacteria culture device is lack of sterilization facilities.

In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a bacteria culture case and application method with adjustable, includes the working plate, the fixed column is installed to the bottom of working plate, the top fixed mounting of working plate has the booster pump, the top of booster pump is run through and is installed the booster pipe, the decompression pump is installed at the top of working plate, the top of decompression pump is run through and is installed the decompression pipe, the top fixed mounting of working plate has the fixed plate, oxygen generator, carbon dioxide generator and nitrogen generator are installed in proper order to the top of fixed plate, the oxygen hose is installed at the top of oxygen generator, the fixed surface of oxygen hose installs the oxygen valve, carbon dioxide gas pipe is installed at the top of carbon dioxide generator, carbon dioxide gas pipe's surface runs through installs the carbon dioxide gas valve, nitrogen generator's top runs through installs the nitrogen gas pipeline, nitrogen gas pipeline's surface runs through installs the nitrogen gas valve, the top of the working plate is provided with a heat conducting disc in a penetrating manner, the top of the heat conducting disc is fixedly provided with an incubator, the top of the incubator is fixedly provided with a temperature adjusting cover, the top of the temperature adjusting cover is provided with a recess, the opening of the recess is provided with a pull rod, the top of the temperature adjusting cover is provided with a temperature adjusting knob, the top of the temperature adjusting cover is provided with a blowing machine in a penetrating manner, one side of the incubator is provided with an air pressure gauge, the side surface of the incubator is embedded with a light-transmitting plate, the inside of the light-transmitting plate is provided with a friction shaft in a penetrating manner, the surface of the friction shaft is provided with an eccentric light barrier, one side of the eccentric light barrier is fixedly provided with a short rod, the bottom of the working plate is provided with a sliding groove through a screw, a T-shaped limiting plate is arranged inside the sliding groove through a, the mounting bracket is installed at the top of connecting plate, the mounting bracket is installed through the loose axle and is stabilized the connecting plate, the backup pad is installed to the bottom of stabilizing the connecting plate, the inside of backup pad is run through and is installed the dish that disinfects, the front of incubator is installed and is passed through the heat transfer dish.

Preferably, one end of the pressure increasing pipe penetrates through the incubator, the pressure reducing pipe is a hollow round pipe, and one end of the pressure reducing pipe is located inside the incubator.

Preferably, the connecting parts of the oxygen pipe, the carbon dioxide gas pipe and the nitrogen pipeline with the incubator are all provided with sealing rings, and one ends of the oxygen pipe, the carbon dioxide gas pipe and the nitrogen pipeline with the incubator are respectively located in the incubator.

Preferably, the light-transmitting plates are symmetrically arranged relative to the incubator, and the two light-transmitting plates are movably connected with an eccentric light barrier through a friction shaft respectively.

Preferably, the side face and the top face of the sliding chute are respectively provided with two rectangular notches, and the number of the sliding chutes is two.

Preferably, the other end of the linkage rod is fixedly connected with one side of the other T-shaped limiting plate, and the linkage rod is matched with the notch on the side face of the sliding groove.

Preferably, the connecting plate is L-shaped, and the bottom surface of the connecting plate is parallel to the horizontal plane.

Preferably, the sterilization disc and the heat conduction disc are coaxially arranged, and the heating wire is installed at the top of the sterilization disc.

Compared with the prior art, the invention has the beneficial effects that:

1. through setting gradually oxygen generator, carbon dioxide generator and nitrogen generator, can provide aerobic environment and anaerobic environment for the incubator to make the adaptability of this device obtain very big promotion, through the cultivation process that has made things convenient for the bacterium, realized the regulation of oxygen, carbon dioxide and nitrogen gas proportion, through setting up booster pump and decompression pump, can adjust the atmospheric pressure size of incubator inside, thereby make the environmental variable of bacteria cultivation more be convenient for adjust.

2. Through the pull ring of pulling backup pad one end, can remove the position of dish that disinfects, before the incubator uses, the inside of high temperature that the inside heating wire of dish that disinfects produced further transmits to the incubator through the heat conduction dish, thereby can disinfect for the inside radiation in center in the bottom of incubator, can pull the backup pad and the dish that disinfects to one side of working plate through further pulling pull ring, can be with the ninety degrees angle of backup pad rotation through the inside loose axle of mounting bracket, thereby it disinfects to carry out the heat radiation as the center to the incubator inside with the side of incubator, thereby be favorable to guaranteeing the sterile environment of incubator.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:

FIG. 1 is a schematic view of the complete structure of the present invention;

FIG. 2 is a rear view structural diagram of the present invention;

FIG. 3 is a left side perspective view of the present invention;

FIG. 4 is a side view of the present invention;

FIG. 5 is a bottom view block diagram of the present invention;

FIG. 6 is a front view of the mounting bracket attachment of the present invention;

fig. 7 is a view showing a construction of the mounting bracket connection of the present invention.

In the figure: 1, working plate; 2, fixing a column; 3, a booster pump; 4, pressurizing the pipe; 5, a decompression pump; 6, a decompression tube; 7 fixing the plate; 8, an oxygen generator; 9 a carbon dioxide generator; 10 a nitrogen generator; 11 an oxygen tube; 12 an oxygen valve; 13 a carbon dioxide gas pipe; 14 a carbon dioxide gas valve; 15 nitrogen gas pipeline; a 16 nitrogen gas valve; 17 a heat conducting plate; 18 an incubator; 19 a temperature adjusting cover; 20, sinking; 21 a pull rod; 22 a temperature adjustment knob; 23 an air blower; 24 air pressure gauge; 25 light-transmitting plates; 26 a friction shaft; 27 an eccentric light barrier; 28 short rods; 29 chute; a 30T-shaped limiting plate; 31 a trace; 32 connecting plates; 33, mounting a frame; 34 stabilizing the web; 35 a support plate; 36, sterilizing the disc; 37 heat transfer plates.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-7, the present invention provides the following technical solutions: an adjustable bacteria incubator and a using method thereof, comprising a working plate 1, wherein a fixed column 2 is arranged at the bottom of the working plate 1, a booster pump 3 is fixedly arranged at the top of the working plate 1, a booster pipe 4 is arranged at the top of the booster pump 3 in a penetrating way, a pressure reducing pump 5 is arranged at the top of the working plate 1, a pressure reducing pipe 6 is arranged at the top of the pressure reducing pump 5 in a penetrating way, a fixed plate 7 is fixedly arranged at the top of the working plate 1, an oxygen generator 8, a carbon dioxide generator 9 and a nitrogen generator 10 are sequentially arranged at the top of the fixed plate 7, an oxygen pipe 11 is arranged at the top of the oxygen generator 8, an oxygen valve 12 is fixedly arranged on the surface of the oxygen pipe 11, a carbon dioxide gas pipe 13 is arranged at the top of the carbon dioxide generator 9, a carbon dioxide gas valve 14, a nitrogen valve 16 is installed on the surface of the nitrogen pipeline 15 in a penetrating manner, a heat conducting disc 17 is installed on the top of the working plate 1 in a penetrating manner, an incubator 18 is fixedly installed on the top of the heat conducting disc 17, a temperature adjusting cover 19 is fixedly installed on the top of the incubator 18, a recess 20 is arranged on the top of the temperature adjusting cover 19, a pull rod 21 is installed at the opening of the recess 20, a temperature adjusting knob 22 is installed on the top of the temperature adjusting cover 19, a blower 23 is installed on the top of the temperature adjusting cover 19 in a penetrating manner, an air pressure gauge 24 is installed on one side of the incubator 18, a light transmitting plate 25 is embedded on the side surface of the incubator 18, a friction shaft 26 is installed in the light transmitting plate 25 in a penetrating manner, an eccentric light blocking plate 27 is installed on the surface of the friction shaft 26, a short rod 28 is fixedly installed on one side of the eccentric light blocking plate 27, a sliding groove, the top of T word limiting plate 30 has connecting plate 32 through screwed connection, and mounting bracket 33 is installed at the top of connecting plate 32, and mounting bracket 33 installs through the loose axle and stabilizes connecting plate 34, stabilizes the bottom of connecting plate 34 and installs backup pad 35, and the inside of backup pad 35 is run through and is installed sterilization dish 36, and heat conduction dish 37 is installed in the front of incubator 18.

In this embodiment, high temperature generated by the heating wire inside the sterilizing tray 36 is transferred to the heat conducting tray 17, and further transferred to the inside of the incubator 18 through the heat conducting tray 17, so that radiation sterilization can be performed to the inside of the incubator 18, the pull ring is further pulled, the stabilizing connecting plate 34 rotates by ninety degrees around the movable shaft inside the mounting bracket 33, at this time, heat radiation sterilization is performed to the inside of the incubator 18 centering on the side surface of the incubator 18, corresponding gas can be generated by the three gas generators by installing the oxygen generator 8, the carbon dioxide generator 9 and the nitrogen generator 10 on the top of the working plate 1, so that a suitable gas environment is provided during the bacterial culture process, the use of the bacterial incubator 18 is greatly facilitated, the adjustment of the proportion of oxygen, carbon dioxide and nitrogen is further realized, the gas pressure inside the incubator 18 can be increased by the booster pump 3, similarly, the decompression pump 5 can reduce the internal pressure of the incubator 18, thereby facilitating adjustment of the internal pressure of the incubator 18 and improving the adjustability of the incubator 18 from many aspects.

Specifically, one end of the pressure increasing pipe 4 penetrates the inside of the incubator 18, the pressure reducing pipe 6 is a hollow circular pipe, one end of the pressure reducing pipe 16 is located inside the incubator 18, the pressure increasing pipe 4 introduces the pressure increasing gas generated by the pressure increasing pump 3 into the incubator 18, the pressure inside the incubator 18 can be increased, and the pressure reducing pipe 16 cooperates with the pressure reducing pump 5, so that the pressure inside the incubator 18 can be reduced.

Specifically, the connecting portions of the oxygen tube 11, the carbon dioxide gas tube 13, the nitrogen pipeline 15 and the incubator 18 are provided with sealing rings, one ends of the oxygen tube 11, the carbon dioxide gas tube 13, the nitrogen pipeline 15 and the incubator 18 are respectively located inside the incubator 18, and one ends of the oxygen tube 11, the carbon dioxide gas tube 13, the nitrogen pipeline 15 and the incubator 18 penetrate through the inside of the incubator 18, so that oxygen, carbon dioxide and nitrogen can be respectively conveyed inside the incubator 18, and the gas variable of the bacteria culture environment can be conveniently adjusted.

Specifically, the light-transmitting plates 25 are symmetrically arranged about the incubator 18, and the two light-transmitting plates 25 are movably connected with an eccentric light barrier 27 through a friction shaft 26 respectively, and the light-transmitting area of the light-transmitting plates 25 can be adjusted by rotating the eccentric light barrier 27, so as to supplement necessary illumination for the inside of the incubator 18.

Specifically, rectangular notches are respectively formed in the side faces and the top face of the sliding groove 29, two sliding grooves 29 are formed in the sliding groove 29, and the stable movement of the T-shaped limiting plate 30 inside the sliding groove 29 can be guaranteed by the arrangement of the two sliding grooves 29.

Specifically, the other end of the linkage rod 31 is fixedly connected with one side of another T-shaped limiting plate 30, the linkage rod 31 is matched with the notches on the side surfaces of the sliding grooves 29, and the two symmetrical T-shaped limiting plates 30 are connected with each other through the linkage rod 31, so that the two T-shaped limiting plates 30 are kept to move simultaneously, and the sterilization disc 36 is prevented from being inclined in the moving process.

Specifically, the connecting plate 32 is the L style of calligraphy, and the bottom surface and the horizontal plane of connecting plate 32 are parallel to each other, through the connecting plate 32 that sets up the L style of calligraphy, are favorable to increasing the area of contact with backup pad 35 than the rectangular plate to firm being connected with backup pad 35.

Specifically, the sterilization tray 36 and the heat conduction tray 17 are coaxially arranged, the heating wire is installed at the top of the sterilization tray 36, and the sterilization tray 36 and the heat conduction tray 17 are coaxially arranged, so that a good heat radiation sterilization environment is formed in the incubator 18.

The working principle and the using method of the invention are as follows: after the sterilization tray 36 is installed, when the sterilization tray is used, the sterilization tray 36 is electrically connected with an external power supply, the electric heating wire in the sterilization tray 36 can be fully heated and heated, the sterilization tray 36 and the heat conduction tray 17 are arranged coaxially, heat can be transferred to the inside of the incubator 18 through the heat conduction tray 17, so that the gas environment in the incubator 18 before use can be fully sterilized, the pull ring at one end of the support plate 35 is further pulled, the sterilization tray 36 and the heat conduction tray 37 rotate through an angle ninety degrees around the movable shaft in the mounting rack 33 through the stable connection plate 34, at the moment, the sterilization tray 36 and the heat conduction tray 37 are concentric and coaxial, so that the heat radiation sterilization is carried out to the inside of the incubator 18 by taking the side surface of the incubator 18 as the center, and the sterile environment.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art will understand that various changes, modifications and substitutions can be made without departing from the spirit and scope of the invention as defined by the appended claims. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The utility model provides a bacteria culture case and application method with adjustable, includes working plate (1), fixed column (2) are installed to the bottom of working plate (1), its characterized in that: the top fixed mounting of working plate (1) has booster pump (3), the top of booster pump (3) is run through and is installed booster pipe (4), decompression pump (5) is installed at the top of working plate (1), the top of decompression pump (5) is run through and is installed decompression pipe (6), the top fixed mounting of working plate (1) has fixed plate (7), oxygen generator (8), carbon dioxide generator (9) and nitrogen generator (10) are installed in proper order to the top of fixed plate (7), oxygen hose (11) are installed at the top of oxygen generator (8), the fixed surface of oxygen hose (11) installs oxygen valve (12), carbon dioxide gas pipe (13) is installed at the top of carbon dioxide generator (9), carbon dioxide gas valve (14) are installed in the surface run through of carbon dioxide gas pipe (13), the top of nitrogen generator (10) runs through and installs nitrogen gas pipeline (15), the surface of nitrogen gas pipeline (15) runs through and installs nitrogen gas valve (16), the top of working plate (1) runs through and installs heat conduction dish (17), the top fixed mounting of heat conduction dish (17) has incubator (18), the top fixed mounting of incubator (18) has temperature regulation lid (19), the top of temperature regulation lid (19) is provided with sunken (20), pull rod (21) are installed to the opening part of sunken (20), adjust the temperature knob (22) are installed at the top of temperature regulation lid (19), the top of temperature regulation lid (19) is run through and is installed blower (23), atmospheric pressure scale (24) are installed to one side of incubator (18), the side of incubator (18) is inlayed and is equipped with light-passing plate (25), the inside of light-passing plate (25) is run through and is installed friction axle (26), an eccentric light barrier (27) is arranged on the surface of the friction shaft (26), a short rod (28) is fixedly arranged on one side of the eccentric light barrier (27), the bottom of the working plate (1) is provided with a sliding chute (29) through a screw, a T-shaped limiting plate (30) is arranged inside the sliding chute (29) through a rectangular notch, one side of the T-shaped limit plate (30) is connected with a linkage rod (31), the top of the T-shaped limit plate (30) is connected with a connecting plate (32) through a screw, the top of the connecting plate (32) is provided with an installation frame (33), the installation frame (33) is provided with a stable connecting plate (34) through a movable shaft, the bottom of stabilizing connecting plate (34) is installed backup pad (35), the inside of backup pad (35) is run through and is installed sterilization dish (36), heat transfer dish (37) are installed in the front of incubator (18).

2. The adjustable bacteria incubator and the use method thereof according to claim 1, wherein: one end of the pressure increasing pipe (4) penetrates through the interior of the incubator (18), the pressure reducing pipe (6) is a hollow round pipe, and one end of the pressure reducing pipe (16) is located in the interior of the incubator (18).

3. The adjustable bacteria incubator and the use method thereof according to claim 1, wherein: the connecting parts of the oxygen pipe (11), the carbon dioxide gas pipe (13), the nitrogen pipeline (15) and the incubator (18) are all provided with sealing rings, and one ends of the oxygen pipe (11), the carbon dioxide gas pipe (13), the nitrogen pipeline (15) and the incubator (18) are respectively positioned in the incubator (18).

4. The adjustable bacteria incubator and the use method thereof according to claim 1, wherein: the light-transmitting plates (25) are symmetrically arranged relative to the incubator (18), and the two light-transmitting plates (25) are movably connected with an eccentric light barrier (27) through a friction shaft (26) respectively.

5. The adjustable bacteria incubator and the use method thereof according to claim 1, wherein: rectangular notches are formed in the side face and the top face of the sliding groove (29) respectively, and two sliding grooves (29) are formed.

6. The adjustable bacteria incubator and the use method thereof according to claim 1, wherein: the other end of the linkage rod (31) is fixedly connected with one side of the other T-shaped limiting plate (30), and the linkage rod (31) is matched with the groove opening on the side surface of the sliding groove (29).

7. The adjustable bacteria incubator and the use method thereof according to claim 1, wherein: the connecting plate (32) is L-shaped, and the bottom surface of the connecting plate (32) is parallel to the horizontal plane.

8. The adjustable bacteria incubator and the use method thereof according to claim 1, wherein: the sterilization disc (36) and the heat conduction disc (17) are coaxially arranged, and a heating wire is installed at the top of the sterilization disc (36).