CN111203292B - Micro-pipette and pipetting method - Google Patents
Micro-pipette and pipetting method Download PDFInfo
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- CN111203292B CN111203292B CN202010089729.6A CN202010089729A CN111203292B CN 111203292 B CN111203292 B CN 111203292B CN 202010089729 A CN202010089729 A CN 202010089729A CN 111203292 B CN111203292 B CN 111203292B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Sampling And Sample Adjustment (AREA)
Abstract
The embodiment of the utility model discloses a micropipette and a pipetting method, comprising a pipette barrel, wherein a liquid taking component for taking liquid and pipetting and a translational capacity adjusting device for adjusting capacity are arranged in the pipette barrel, the liquid taking component comprises a liquid taking rod vertically penetrating into the pipette barrel, the upper end of the liquid taking rod extends out of the pipette barrel and is provided with a liquid taking pressing plate, a reset spring is arranged between the liquid taking pressing plate and the top end of the pipette barrel, the lower end of the liquid taking rod is provided with a piston, the bottom of the pipette barrel is connected with a suction head, an anti-unhook hook is arranged outside the pipette barrel, the translational capacity adjusting device comprises an adjusting barrel, an adjusting gear and two rotating rods, the adjusting barrel and the adjusting gear are rotatably sleeved outside the liquid taking rod through bearings, a sealing adjusting disc is sleeved outside the adjusting barrel, one hand can operate the pipette, and convenience is brought to experimental staff.
Description
Technical Field
The embodiment of the utility model relates to the field of medical equipment, in particular to a micropipette and a pipetting method.
Background
The micro liquid-transfering device is a precision liquid-taking device (commonly called gun) which can be regulated at will in a certain volume range, and its basic principle is that the piston in the device can be moved up and down, the moving distance of air piston is implemented by means of regulating wheel control screw structure, and the push button can be pushed to drive push rod to make piston move downwards so as to remove air in the piston cavity. After the hands are loosened, the piston returns to the original position under the action of the return spring, so that the liquid suction process is completed once.
The micropipette is a necessary tool for biological experiments, so that an experimenter is required to master the use skill, and in the process of learning to use the micropipette, a novice hopes to master the 'quantity' of the exercise progress, namely, the time measurement required by one standard pipetting operation is completed, but the conventional micropipette cannot measure the exercise progress of the novice, so that people cannot objectively and accurately evaluate the exercise progress.
The Chinese patent application No. 201620109601.0 discloses a liquid transfer device for detecting traditional Chinese medicinal materials, which comprises a liquid transfer main body, a suction nozzle and a button, wherein the suction nozzle is arranged below the liquid transfer main body, and the button is arranged above the liquid transfer main body; the button comprises a push rod and a top cap; the pipetting body is provided with a dosage adjusting device for adjusting the displacement of the button, and the dosage adjusting device comprises a knob. In this pipettor, the button for getting liquid and the knob setting that is used for adjusting the capacity are at the top, this pipettor still is provided with the couple simultaneously, conveniently hang and lean on at the hand edge, in normal use, the couple is hung and is arranged in four finger departments, thumb press button carries out the liquid operation of getting liquid, and when carrying out capacity adjustment, need rotate capacity adjustment knob through forefinger and thumb and carry out capacity adjustment, need forefinger and thumb and adjust knob contact in the adjustment process, the thumb is located the outside of pipettor main part and couple, can contact with adjust knob, and the forefinger is located between pipettor main part and the couple, can't lift upwards, just can't also be contacted with adjust knob, and then make capacity adjustment process can't realize, consequently, need adjust the position of couple through another hand, rotate the pipettor and make the couple remove to the tiger's mouth department between forefinger and the thumb, rethread forefinger and thumb rotate capacity adjustment knob, perhaps, the position of not adjusting the couple still is hung and is arranged in four finger departments, directly rotate capacity adjustment knob through another hand.
That is, in the prior art, there are a plurality of processes in the use of the pipette, namely, a normal use process and a capacity adjustment process, angles of the pipette in hands are different in different processes, and as fingers are clamped between the pipette body and the hooks, the hands holding the pipette body cannot change the angles of the pipette, and the other hand is needed to assist, so that the operation of one hand is inconvenient when the capacity adjustment is performed, two hands are needed to cooperate, and inconvenience is brought to an experimenter and an experimental process.
Disclosure of Invention
Therefore, the embodiment of the utility model provides a micropipette and a pipetting method, which are used for solving the problems that the conventional micropipette is inconvenient to operate by one hand when capacity is adjusted, two hands are needed to be matched for carrying out, and inconvenience is brought to an experimenter and an experiment process.
In order to achieve the above object, the embodiments of the present utility model provide the following technical solutions:
the micropipette comprises a pipette barrel, wherein a liquid taking component for taking liquid and transferring liquid and a translational capacity adjusting device for adjusting capacity are arranged in the pipette barrel;
the liquid taking assembly comprises a liquid taking rod vertically penetrating through the pipette barrel, the upper end of the liquid taking rod extends out of the pipette barrel and is provided with a liquid taking pressing plate, a reset spring is arranged between the liquid taking pressing plate and the top end of the pipette barrel, the lower end of the liquid taking rod is provided with a piston, the bottom of the pipette barrel is connected with a suction head, and an anti-unhooking hook is arranged outside the pipette barrel;
the translational capacity adjusting device comprises an adjusting cylinder, an adjusting gear and two rotating rods, wherein the adjusting cylinder and the adjusting gear are rotatably sleeved outside the liquid taking rod through bearings, and a sealing adjusting disc is sleeved outside the adjusting cylinder through threads.
As a preferable scheme of the utility model, the rotating rods are positioned in the pipette cylinder and above the sealing adjusting disc, the rotating rods are arranged in parallel up and down, a synchronous belt is wound between the two rotating rods, the tail ends of the rotating rods are connected with a transmission bevel gear, and the transmission bevel gear above is meshed with the adjusting gear.
As a preferable scheme of the utility model, an adjusting groove is arranged on the outer side wall of the pipette barrel, an adjusting plate is slidably arranged in the adjusting groove, a driving rack is arranged in the pipette barrel, the adjusting plate is connected with the driving rack, and the driving rack is meshed with the transmission bevel gear below.
As a preferable scheme of the utility model, the bottom of the adjusting groove is provided with an adjusting slit, the adjusting groove is communicated with the inner cavity of the pipette cylinder through the adjusting slit, the adjusting plate is connected with a driving plate, and the driving plate passes through the adjusting slit and is connected with the driving rack.
As a preferable scheme of the utility model, the regulating cylinder and the regulating gear are both positioned in the pipette cylinder and are sequentially arranged from top to bottom, and the upper end of the regulating cylinder is fixedly connected with the lower end face of the regulating gear.
As a preferred embodiment of the present utility model, a seal ring is provided between the edge of the seal adjustment plate and the inner wall of the pipette cylinder.
As a preferable scheme of the utility model, the outer surface of the adjusting plate is provided with grains matched with the finger surface of the thumb, the outer wall of the pipette barrel is provided with a holding plate, the holding plate is provided with undulating grains matched with fingers, and the anti-falling hook is in an inverted L shape and is arranged on the holding plate
The utility model also provides a micropipette method based on the micropipette, comprising the steps of:
s100, adjusting the capacity of the pipettor: a sealing adjusting disc is arranged in the pipette cylinder, an accommodating space of the pipette cylinder is formed between the sealing adjusting disc and the bottom of the pipette cylinder, and the accommodating space of the pipette cylinder is changed by changing the height of the sealing adjusting disc, namely, the capacity of the pipette is adjusted;
and S200, after capacity adjustment is completed, taking liquid and pipetting by using a pipettor.
As a preferable scheme of the utility model, the specific adjusting process of the capacity of the liquid dispenser is as follows:
s101, hanging the anti-unhooking hook on four fingers except for a thumb, wherein the thumb is positioned opposite to the anti-unhooking hook and is pressed on an adjusting plate;
s102, holding the pipette cylinder by four fingers in cooperation with a palm, pushing the adjusting plate to move by the thumb, driving the translational capacity adjusting device, and changing the height of the sealing adjusting disc.
As a preferable scheme of the utility model, the specific process of liquid taking and transferring is as follows;
s201, after capacity adjustment is completed, holding the pipette barrel by four fingers, then lifting the thumb to the liquid taking pressing plate, pressing the liquid taking pressing plate, compressing the reset spring, driving the piston to move downwards through the liquid taking rod, extruding air and enabling the air to escape from an outlet at the lower end of the suction head until the air is completely discharged after the piston moves to the bottommost part;
s202, moving the liquid taker to the upper part of the liquid to be taken, and inserting the suction head into the liquid to be taken;
s203, loosening the thumb, recovering the liquid taking pressing plate to be in situ under the action of the elastic force of the reset spring, moving the piston upwards, and sucking liquid to be taken into the pipette barrel to finish liquid taking operation;
s204, moving the liquid extractor to the position above the detection container;
s205, pressing the liquid taking pressing plate, and discharging the solution in the pipette barrel into the detection container to finish liquid taking and pipetting operations.
Embodiments of the present utility model have the following advantages:
the utility model adopts a pushing adjusting mode to replace a rotating adjusting mode in the prior art, so that the liquid taking process and the capacity adjusting process of the liquid dispenser can be operated by one hand, and meanwhile, the angle of the liquid dispenser is not required to be adjusted in the transitional process of the two operations, and the liquid dispenser still keeps at the original position, thereby avoiding the defects that the normal use process of the liquid dispenser and the capacity adjusting process in the prior art are different in angle in hands and the other hand is required to help, enabling one hand to finish all operations of the liquid dispenser, freeing one hand of an experimenter, improving the operability and the practicability of the liquid dispenser and bringing convenience to experimenters and experimental processes.
Drawings
In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It will be apparent to those of ordinary skill in the art that the drawings in the following description are exemplary only and that other implementations can be obtained from the extensions of the drawings provided without inventive effort.
The structures, proportions, sizes, etc. shown in the present specification are shown only for the purposes of illustration and description, and are not intended to limit the scope of the utility model, which is defined by the claims, so that any structural modifications, changes in proportions, or adjustments of sizes, which do not affect the efficacy or the achievement of the present utility model, should fall within the ambit of the technical disclosure.
FIG. 1 is a schematic diagram of the overall structure of an embodiment of the present utility model;
FIG. 2 is a schematic view of a liquid extraction module according to an embodiment of the present utility model;
fig. 3 is a schematic structural diagram of a translational capacity adjustment device according to an embodiment of the present utility model.
In the figure:
1-a pipette cartridge; 2-translational capacity adjusting device; 3-a liquid taking component;
201-an adjustment cylinder; 202-adjusting gears; 203-sealing a conditioning disk; 204-rotating the rod; 205-a synchronous belt; 206-a drive bevel gear; 207-an adjustment tank; 208-adjusting plate; 209-drive rack; 210-adjusting the seam; 211-a drive plate; 212-grip plate; 213-sealing ring;
301-a liquid taking rod; 302, liquid taking and pressing plate; 303-a return spring; 304-a piston; 305-tip; 306-unhooking prevention.
Detailed Description
Other advantages and advantages of the present utility model will become apparent to those skilled in the art from the following detailed description, which, by way of illustration, is to be read in connection with certain specific embodiments, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
As shown in fig. 1 to 3, the embodiment of the utility model provides a micropipette, which comprises a pipette barrel 1, wherein a liquid taking component 3 for taking liquid and transferring liquid and a translational capacity adjusting device 2 for adjusting capacity are arranged in the pipette barrel 1, the liquid taking component comprises a liquid taking rod 301 vertically penetrating through the pipette barrel 1, the upper end of the liquid taking rod 301 extends out of the pipette barrel 1 and is provided with a liquid taking pressing plate 302, a reset spring 303 is arranged between the liquid taking pressing plate 302 and the top end of the pipette barrel 1, a piston 304 is arranged at the lower end of the liquid taking rod 301, a tip 305 is connected to the bottom of the pipette barrel 1, and an anti-falling hook 306 is arranged outside the pipette barrel 1.
In this embodiment, pressing the liquid-taking pressing plate 302 can drive the liquid-taking rod 301 to move up and down, so as to drive the piston 304 to move up and down, so that the air pressure in the pipette cylinder 1 is changed, when the air pressure is reduced, the solution enters the suction head 305, when liquid taking is performed, the liquid-taking pressing plate 302 is pressed, so that the piston 304 moves to the bottom, air in the pipette cylinder 1 is discharged, then the liquid-taking device is moved to the upper part of the liquid to be taken, the suction head 305 stretches into the liquid to be taken, then the liquid-taking pressing plate 302 is released, the liquid-taking pressing plate 302 moves up under the action of the reset elastic force of the reset spring 303, and the piston 304 is driven to move up through the liquid-taking rod 301, so that the solution is sucked into the pipette cylinder 1, and then the liquid-taking device is moved to the upper part of the detection container, so that the solution is discharged into the detection container, and the transfer of the solution is realized.
The translational capacity adjusting device 2 comprises an adjusting cylinder 201, an adjusting gear 202 and two rotating rods 204, wherein the adjusting cylinder 201 and the adjusting gear 202 are rotatably sleeved outside a liquid taking rod 301 through bearings, and a sealing adjusting disc 203 is sleeved outside the adjusting cylinder 201 in a threaded manner.
In the present embodiment, the seal adjustment plate 203 and the adjustment cylinder 201 constitute a ball screw driving mechanism, wherein the adjustment cylinder 201 corresponds to a screw, the seal adjustment plate 203 corresponds to a screw nut seat, and when the adjustment cylinder 201 rotates, the seal adjustment plate 203 moves in the vertical direction.
In this embodiment, the rotating rods 204 are located in the pipette cylinder 1 and above the sealing adjusting disc 203, the rotating rods 204 are arranged up and down in parallel, a synchronous belt 205 is wound between the two rotating rods 204, the tail ends of the rotating rods 204 are connected with a transmission bevel gear 206, the upper transmission bevel gear 206 is meshed with the adjusting gear 202, an adjusting groove 207 is formed in the outer side wall of the pipette cylinder 1, an adjusting plate 208 is slidably arranged in the adjusting groove 207, a driving rack 209 is arranged in the pipette cylinder 1, the adjusting plate 208 is connected with the driving rack 209, and the driving rack 209 is meshed with the lower transmission bevel gear 206.
In this embodiment, the bottom of adjustment groove 207 is provided with adjustment slit 210, adjustment groove 207 passes through adjustment slit 210 and the inner chamber intercommunication of pipette section of thick bamboo 1, and adjusting plate 208 is connected with drive plate 211, and drive plate 211 passes adjustment slit 210 and links to each other with drive rack 209, and adjustment section of thick bamboo 201, adjusting gear 202 and sealed adjustment disk 203 all are located pipette section of thick bamboo 1, and from top to bottom set gradually, and the upper end of adjustment section of thick bamboo 201 links to each other with the lower terminal surface of adjusting gear 202 is fixed, and the lower extreme of adjustment section of thick bamboo 201 links to each other with the upper end is fixed of sealed adjustment disk 203, is provided with sealing washer 213 between the limit portion of sealed adjustment disk 203 and the inner wall of pipette section of thick bamboo 1.
In this embodiment, the outer surface of the adjusting plate 208 is provided with lines matching with the finger surface of the thumb, the outer wall of the pipette barrel 1 is provided with a holding plate 212, the holding plate 212 is provided with undulating lines matching with the fingers, and the anti-unhook 306 is in an inverted L shape and is disposed on the holding plate 212.
In the translational capacity adjusting device 2 of this embodiment, the up-and-down movement of the adjusting plate 208 drives the driving rack 209 to move through the driving plate 211, the driving bevel gear 206 is rotated by utilizing the meshing transmission relationship between the driving rack 209 and the lower driving bevel gear 206, the upper driving bevel gear 206 is driven to rotate by utilizing the synchronous rotation relationship between the two rotating rods 204, and then the adjusting cylinder 201 is driven to rotate by utilizing the meshing transmission relationship between the upper driving bevel gear 206 and the adjusting gear 202, so that the sealing adjusting disk 203 is driven to move in the vertical direction, and the capacity of the pipette cylinder 1 is changed.
According to the embodiment, the translational capacity adjusting device 2 is arranged, so that capacity adjustment can be performed in a pushing mode, when a pipette is used for pipetting operation, an experimenter holds the pipette with one hand, when the capacity adjustment is needed, on the basis that the experimenter holds the pipette with one hand in the process, the thumb is opened, the rest four fingers are matched with the palm, the pipette is still held with one hand, then the translational capacity adjusting device 2 is operated by the thumb, and the thumb can be pushed by a single finger due to the pushing mode.
Since the present embodiment uses the pushing method to perform the capacity adjustment, only the thumb needs to be moved when performing the capacity adjustment, and when using the pipette, the four fingers are positioned between the pipette tube and the unhooking prevention hook 306 and are restrained, but the thumb is not positioned between the pipette tube and the unhooking prevention hook 306 and can freely move, so that the capacity adjustment process of the pipette can be performed by one hand.
As described above, the two operation procedures of the pipette in this embodiment are: the liquid taking process and the capacity adjusting process can be operated by one hand, meanwhile, in the transition process of the two operations, the angle of the liquid dispenser is not required to be adjusted, the liquid dispenser is still kept at the original position, the phenomenon that the angle of the liquid dispenser in the hand is different in the normal use process and the capacity adjusting process in the prior art, the other hand is required to assist, the one-hand operation cannot be completed, the inconvenience is brought to an experimenter and an experiment process, and the operability performance of the liquid dispenser is improved.
Therefore, the micropipette in the embodiment can be conveniently operated by one hand, and the other hand is not needed in the whole process, so that one hand is liberated in the experimental process, and convenience is brought to the experimenter and the experimental process.
The embodiment adopts a pushing adjusting mode to replace a rotating adjusting mode in the prior art, so that the single-hand operation of the liquid dispenser is realized, and the operability and the practicability of the liquid dispenser are improved.
As shown in fig. 1 to 2, the present utility model further provides a micropipette method, which is characterized in that the micropipette method is based on a micropipette, the micropipette comprises a pipette cylinder 1, a liquid taking component 3 for taking liquid and transferring liquid and a translational capacity adjusting device 2 for adjusting capacity are arranged in the pipette cylinder 1, the liquid taking component comprises a liquid taking rod 301 vertically penetrating through the pipette cylinder 1, the upper end of the liquid taking rod 301 extends out of the pipette cylinder 1 and is provided with a liquid taking pressing plate 302, a reset spring 303 is arranged between the liquid taking pressing plate 302 and the top end of the pipette cylinder 1, a piston 304 is arranged at the lower end of the liquid taking rod 301, a suction head 305 is connected to the bottom of the pipette cylinder 1, an anti-falling hook 306 is arranged outside the pipette cylinder 1, the translational capacity adjusting device 2 comprises an adjusting cylinder 201, an adjusting gear 202 and two rotating rods 204, the adjusting cylinder 201 and the adjusting gear 202 are rotatably sleeved outside the liquid taking rod 301 through bearings, and a sealing adjusting disk 203 is sleeved outside the adjusting cylinder 201.
The micropipetting method comprises the following steps:
s100, adjusting the capacity of the pipettor: firstly, a sealing adjusting disc is arranged in a pipette barrel, an accommodating space of the pipette barrel is formed between the sealing adjusting disc and the bottom of the pipette barrel, the accommodating space of the pipette barrel is changed by changing the height of the sealing adjusting disc, secondly, an anti-falling hook 306 is hung on four fingers except for a thumb, the thumb is positioned opposite to the anti-falling hook 306 and presses on an adjusting plate 208, finally, the four fingers are matched with a palm to hold the pipette barrel 1, the thumb pushes the adjusting plate 208 to move, and the translational capacity adjusting device 2 is driven to change the capacity of a pipette;
s200, after capacity adjustment is completed, taking liquid and pipetting by using a pipettor: firstly, after capacity adjustment is completed, holding the pipette cylinder 1 by four fingers, then lifting the thumb to the position of the liquid taking pressing plate 302, pressing the liquid taking pressing plate 302, compressing the return spring 303, driving the piston 304 to move downwards through the liquid taking rod 301, extruding air and enabling the air to escape from an outlet at the lower end of the suction head until the piston 304 moves to the bottommost part so that the air is completely discharged, secondly, moving the liquid taking device to the position above the liquid to be taken, inserting the suction head into the liquid to be taken, and then loosening the thumb, wherein the liquid taking pressing plate 302 returns to the original position under the elastic force of the return spring 303, the piston 304 moves upwards and pumps the liquid to be taken into the pipette cylinder 1, thus completing liquid taking operation, and moving the liquid taking device to the position above the detection container from time to time; finally, the liquid-taking pressing plate 302 is pressed, and the solution in the pipette cylinder 1 is discharged into the detection container, thereby completing the liquid-taking and pipetting operations.
While the utility model has been described in detail in the foregoing general description and specific examples, it will be apparent to those skilled in the art that modifications and improvements can be made thereto. Accordingly, such modifications or improvements may be made without departing from the spirit of the utility model and are intended to be within the scope of the utility model as claimed.
Claims (7)
1. The micropipette is characterized by comprising a pipette barrel (1), wherein a liquid taking component (3) for taking liquid and transferring liquid and a translational capacity adjusting device (2) for adjusting capacity are arranged in the pipette barrel (1);
the liquid taking assembly comprises a liquid taking rod (301) vertically penetrating through the pipette barrel (1), the upper end of the liquid taking rod (301) extends out of the pipette barrel (1) and is provided with a liquid taking pressing plate (302), a reset spring (303) is arranged between the liquid taking pressing plate (302) and the top end of the pipette barrel (1), a piston (304) is arranged at the lower end of the liquid taking rod (301), a suction head (305) is connected to the bottom of the pipette barrel (1), and an anti-falling hook (306) is arranged outside the pipette barrel (1);
the translational capacity adjusting device (2) comprises an adjusting cylinder (201), an adjusting gear (202) and two rotating rods (204), wherein the adjusting cylinder (201) and the adjusting gear (202) are rotatably sleeved outside the liquid taking rod (301) through bearings, and a sealing adjusting disc (203) is sleeved outside the adjusting cylinder (201) through threads;
the rotating rods (204) are positioned in the pipette cylinder (1) and above the sealing adjusting disc (203), the rotating rods (204) are arranged in parallel up and down, a synchronous belt (205) is wound between the two rotating rods (204), the tail ends of the rotating rods (204) are connected with a transmission bevel gear (206), and the transmission bevel gear (206) above is meshed with the adjusting gear (202); an adjusting groove (207) is formed in the outer side wall of the pipette barrel (1), an adjusting plate (208) is arranged in the adjusting groove (207) in a sliding mode, a driving rack (209) is arranged in the pipette barrel (1), the adjusting plate (208) is connected with the driving rack (209), and the driving rack (209) is meshed with the transmission bevel gear (206) below; the anti-falling hook (306) and the adjusting plate (208) are arranged opposite to each other on the outer side of the pipette barrel (1).
2. The micropipette of claim 1 wherein a bottom of said adjustment slot (207) is provided with an adjustment slot (210), said adjustment slot (207) communicates with an interior cavity of said pipette barrel (1) through said adjustment slot (210), said adjustment plate (208) is connected with a drive plate (211), said drive plate (211) passing through said adjustment slot (210) and being connected with said drive rack (209).
3. The micropipette of claim 1 wherein said adjustment cylinder (201) and said adjustment gear (202) are both located in said pipette cylinder (1) and are disposed in sequence from top to bottom, an upper end of said adjustment cylinder (201) being fixedly connected to a lower end face of said adjustment gear (202).
4. A micropipette as claimed in claim 1, wherein a sealing ring (213) is provided between the edge of the sealing adjustment disc (203) and the inner wall of the pipette barrel (1).
5. The micropipette of claim 4 wherein said adjusting plate (208) has an outer surface provided with a ridge matching a finger surface of a thumb, said pipette barrel (1) has a grip plate (212) on an outer wall thereof, said grip plate (212) has a wavy ridge matching a finger, and said anti-slip hook (306) is inverted-L-shaped and is provided on said grip plate (212).
6. The micropipette pipetting method as recited in claim 1, comprising the steps of:
s100, adjusting the capacity of the pipettor: a sealing adjusting disc is arranged in the pipette cylinder, an accommodating space of the pipette cylinder is formed between the sealing adjusting disc and the bottom of the pipette cylinder, and the accommodating space of the pipette cylinder is changed by changing the height of the sealing adjusting disc, namely, the capacity of the pipette is adjusted;
s101, hanging the anti-unhooking hook on four fingers except for a thumb, wherein the thumb is positioned opposite to the anti-unhooking hook and is pressed on an adjusting plate;
s102, holding the pipette cylinder by four fingers in combination with a palm, pushing the adjusting plate to move by the thumb, driving the translational capacity adjusting device, and changing the height of the sealing adjusting disc;
and S200, after capacity adjustment is completed, taking liquid and pipetting by using a pipettor.
7. The micropipette pipetting method as recited in claim 6, wherein the pipetting process is;
s201, after capacity adjustment is completed, holding the pipette barrel by four fingers, then lifting the thumb to the liquid taking pressing plate, pressing the liquid taking pressing plate, compressing the reset spring, driving the piston to move downwards through the liquid taking rod, extruding air and enabling the air to escape from an outlet at the lower end of the suction head until the air is completely discharged after the piston moves to the bottommost part;
s202, moving the liquid taker to the upper part of the liquid to be taken, and inserting the suction head into the liquid to be taken;
s203, loosening the thumb, recovering the liquid taking pressing plate to be in situ under the action of the elastic force of the reset spring, moving the piston upwards, and sucking liquid to be taken into the pipette barrel to finish liquid taking operation;
s204, moving the liquid extractor to the position above the detection container;
s205, pressing the liquid taking pressing plate, and discharging the solution in the pipette barrel into the detection container to finish liquid taking and pipetting operations.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010089729.6A CN111203292B (en) | 2020-02-13 | 2020-02-13 | Micro-pipette and pipetting method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010089729.6A CN111203292B (en) | 2020-02-13 | 2020-02-13 | Micro-pipette and pipetting method |
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| CN111203292A CN111203292A (en) | 2020-05-29 |
| CN111203292B true CN111203292B (en) | 2023-07-11 |
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| CN114100703A (en) * | 2020-09-01 | 2022-03-01 | 上海脉迪机电技术有限公司 | Sampling swab conservator with pipetting function |
| CN114682318A (en) * | 2020-12-31 | 2022-07-01 | 苏州赛尼特格尔实验室科技有限公司 | Mechanical pipettor |
| CN114838782B (en) * | 2022-05-11 | 2023-05-09 | 嘉兴智正医药科技有限公司 | Multifunctional keratin extractor for eye mask production |
| CN115432212A (en) * | 2022-08-15 | 2022-12-06 | 富瑞奥(东莞)实业有限公司 | Automatic extracting device for perfume split charging |
| CN116764712B (en) * | 2023-08-22 | 2023-10-20 | 福建鸿燕化工有限公司 | Acetic acid liquid taking device |
| CN117427701B (en) * | 2023-12-20 | 2024-03-05 | 建诺为生物技术(江苏)有限公司 | Electric pipettor |
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| DE1942737U (en) * | 1966-05-10 | 1966-07-21 | Edmund Buehler Wissenschaftlic | FAST PIPETTING DEVICE. |
| CH679015A5 (en) * | 1989-06-01 | 1991-12-13 | Socorex Isba S A | Variable volume micropipette - has piston stroke regulator in form of coaxial cylindrical cams with thrust surfaces providing adjustment of piston stroke |
| CN1423747A (en) * | 2000-02-17 | 2003-06-11 | 瑞宁器材公司 | Volume adjustable manual pipette with quick set volume adjustment |
| CN2863136Y (en) * | 2006-01-16 | 2007-01-31 | 北京康富博科技发展有限公司 | Inermous injector with adjustable injection dosage |
| CN101559389A (en) * | 2008-04-17 | 2009-10-21 | 大龙医疗设备(上海)有限公司 | Adjustable pipettor and adjusting device thereof |
| CN202113872U (en) * | 2011-05-13 | 2012-01-18 | 梅方敏 | Adjustable micropipettor |
| CN202983708U (en) * | 2012-11-14 | 2013-06-12 | 宁波拓普森科学仪器有限公司 | Micro-pipettor handle |
| CN105476255A (en) * | 2015-11-18 | 2016-04-13 | 天津市亨必达化学合成物有限公司 | Efficient flask cleaning brush |
| CN205435777U (en) * | 2016-02-03 | 2016-08-10 | 四川华邑检测认证服务有限公司 | A pipettor for chinese -medicinal material detects |
| CN107211744A (en) * | 2017-07-14 | 2017-09-29 | 深圳市星鼎源科技有限公司 | One man operation's trimmer is used in a kind of gardens height trimming |
| CN206980765U (en) * | 2017-07-25 | 2018-02-09 | 江斌 | A kind of intelligent liquid-transfering gun |
| CN108433487A (en) * | 2018-04-10 | 2018-08-24 | 河北工业职业技术学院 | For containing the spoon for scooping thick liquid |
| CN209802754U (en) * | 2019-04-17 | 2019-12-17 | 上海益航生物科技有限公司 | Sampling device for food safety inspection |
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2020
- 2020-02-13 CN CN202010089729.6A patent/CN111203292B/en active Active
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1942737U (en) * | 1966-05-10 | 1966-07-21 | Edmund Buehler Wissenschaftlic | FAST PIPETTING DEVICE. |
| CH679015A5 (en) * | 1989-06-01 | 1991-12-13 | Socorex Isba S A | Variable volume micropipette - has piston stroke regulator in form of coaxial cylindrical cams with thrust surfaces providing adjustment of piston stroke |
| CN1423747A (en) * | 2000-02-17 | 2003-06-11 | 瑞宁器材公司 | Volume adjustable manual pipette with quick set volume adjustment |
| CN2863136Y (en) * | 2006-01-16 | 2007-01-31 | 北京康富博科技发展有限公司 | Inermous injector with adjustable injection dosage |
| CN101559389A (en) * | 2008-04-17 | 2009-10-21 | 大龙医疗设备(上海)有限公司 | Adjustable pipettor and adjusting device thereof |
| CN202113872U (en) * | 2011-05-13 | 2012-01-18 | 梅方敏 | Adjustable micropipettor |
| CN202983708U (en) * | 2012-11-14 | 2013-06-12 | 宁波拓普森科学仪器有限公司 | Micro-pipettor handle |
| CN105476255A (en) * | 2015-11-18 | 2016-04-13 | 天津市亨必达化学合成物有限公司 | Efficient flask cleaning brush |
| CN205435777U (en) * | 2016-02-03 | 2016-08-10 | 四川华邑检测认证服务有限公司 | A pipettor for chinese -medicinal material detects |
| CN107211744A (en) * | 2017-07-14 | 2017-09-29 | 深圳市星鼎源科技有限公司 | One man operation's trimmer is used in a kind of gardens height trimming |
| CN206980765U (en) * | 2017-07-25 | 2018-02-09 | 江斌 | A kind of intelligent liquid-transfering gun |
| CN108433487A (en) * | 2018-04-10 | 2018-08-24 | 河北工业职业技术学院 | For containing the spoon for scooping thick liquid |
| CN209802754U (en) * | 2019-04-17 | 2019-12-17 | 上海益航生物科技有限公司 | Sampling device for food safety inspection |
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|---|---|
| CN111203292A (en) | 2020-05-29 |
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