CN111197005A - Device for preparing anti-aging small molecular peptide - Google Patents

Device for preparing anti-aging small molecular peptide Download PDF

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Publication number
CN111197005A
CN111197005A CN202010031992.XA CN202010031992A CN111197005A CN 111197005 A CN111197005 A CN 111197005A CN 202010031992 A CN202010031992 A CN 202010031992A CN 111197005 A CN111197005 A CN 111197005A
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resistor
connecting pipe
box
operational amplifier
communicated
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CN111197005B (en
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冯启训
张四旗
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Beijing Zhongke Bowling Health Technology Co ltd
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Beijing Kekai And Health Science And Technology Co Ltd
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/52—Mobile; Means for transporting the apparatus
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C12M27/06—Stirrer or mobile mixing elements with horizontal or inclined stirrer shaft or axis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/14—Drying
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    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/16—Sterilization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/20—Heating or cooling

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Abstract

The invention relates to a device for preparing anti-aging small molecular peptides, which comprises the following components: the box, the box upper end is provided with the opening, be provided with the feed box near the opening part in the box, the feed box outer wall passes through the bracing piece with the box inner wall and is connected, the below of feed box is provided with the agitator tank, first connecting pipe one end and feed box bottom intercommunication, the first connecting pipe other end and agitator tank top intercommunication, agitator tank bottom end are provided with row material pipe, the below of arranging the material pipe is provided with the filter screen, the filter screen below is provided with adsorptive separation debitterizing device, adsorptive separation debitterizing device's export below is provided with freeze dryer, freeze dryer's export and sterilization case's feed inlet intercommunication. The invention aims to provide a device for preparing anti-aging small-molecule peptide, which has high extraction rate and high extraction speed.

Description

Device for preparing anti-aging small molecular peptide
Technical Field
The invention belongs to the technical field of small molecule peptides, and particularly relates to a device for preparing anti-aging small molecule peptides.
Background
The small molecular peptide is a biochemical substance between amino acid and protein, has a molecular weight smaller than that of protein and larger than that of amino acid, and is a fragment of protein. Two or more amino acids are linked by peptide bonds to form an "amino acid chain" or "amino acid string" called a peptide. Among them, peptides consisting of 10 to 15 or more amino acids are called polypeptides, peptides consisting of 2 to 9 amino acids are called oligopeptides, and peptides consisting of 2 to 15 amino acids are called small peptides or small peptides. The small molecular peptide has important physiological functions, relates to the fields of hormone, nerve, cell growth and reproduction of human bodies, can regulate the physiological functions of various systems and cells in the human bodies, and maintains the normal physiological activities of the systems of nerve, digestion, reproduction, growth, motor metabolism, circulation and the like of the human bodies. The existing small molecule peptide preparation equipment has low extraction rate of small molecule peptide because raw materials, acid-alkali liquor and clastic enzyme can not be uniformly mixed.
Disclosure of Invention
Therefore, the technical problem to be solved by the invention is to overcome the problem of low extraction rate of small molecule peptides in the prior art.
Therefore, the technical scheme adopted by the invention is that the device for preparing the anti-aging small molecule peptide comprises the following components: the box, the box upper end is provided with the opening, be provided with the feed box near the opening part in the box, feed box outer wall with the box inner wall passes through the bracing piece and connects, the below of feed box is provided with the agitator tank, first connecting pipe one end with feed box bottom intercommunication, the first connecting pipe other end with agitator tank top intercommunication, agitator tank bottom is provided with row material pipe, the below of arranging the material pipe is provided with the filter screen, the filter screen below is provided with adsorptive separation debitterizing ware, adsorptive separation debitterizing ware's export below is provided with freeze dryer, freeze dryer's export and the feed inlet intercommunication of sterilization case.
Preferably, a waste discharge pipe is arranged on the side wall of the adsorption separation debitterizing device.
Preferably, be provided with the groove body on the agitator tank inner wall, the internal first motor that is provided with of recess, the output shaft horizontal direction of first motor sets up, output shaft one end extends to in the agitator tank to connect the pivot of horizontal direction, be provided with a plurality of stirring leaves along circumference on the outer wall of pivot.
Preferably, a sealing cover is arranged at the top end of the box body.
Preferably, the bottom end of the box body is provided with a supporting leg.
Preferably, the bottom end of the supporting leg is provided with a universal wheel.
Preferably, the apparatus further comprises a solution preparing device, wherein the solution preparing device comprises:
the feeding box comprises a cylinder body, a first connecting pipe and a second connecting pipe, wherein the cylinder body is arranged in the vertical direction, a first cylinder body and a second cylinder body are respectively arranged at the upper end and the lower end of the cylinder body, one end of the first connecting pipe is communicated with the side wall of the feeding box, and the other end of the first connecting pipe is communicated with the left end of the second cylinder body;
the first piston is arranged in the first cylinder body and can reciprocate up and down in the first cylinder body;
the second piston is arranged in the second cylinder body and can reciprocate up and down in the second cylinder body;
the fixed plate in the horizontal direction is arranged on the inner wall of the cylinder body, a through hole is formed in the fixed plate, a moving column is arranged in the through hole and can reciprocate up and down along the through hole, one end of the moving column extends into the first cylinder body and is fixedly connected with the first piston, and the other end of the moving column extends into the second cylinder body and is fixedly connected with the second piston;
the movable column penetrates through the flat plate and is fixedly connected with the flat plate, a spring is sleeved on the movable column, one end of the spring is fixedly connected with the flat plate, and the other end of the spring is fixedly connected with the fixed plate;
the second motor is arranged at the top end of the fixing plate, an output shaft of the second motor is connected with one end of a rotating shaft, a cam is arranged at the other end of the rotating shaft, and the top end of the cam is in contact with the bottom end of the flat plate;
one end of the second connecting pipe is communicated with the right end of the second cylinder body, and the other end of the second connecting pipe is communicated with an alkaline liquid tank;
one end of the third connecting pipe is communicated with the right end of the first cylinder body, and the other end of the third connecting pipe is communicated with the acid liquor tank;
one end of the fourth connecting pipe is communicated with the middle of the first connecting pipe, and the other end of the fourth connecting pipe is communicated with the decomposing enzyme liquid tank;
a fifth connecting pipe, fifth connecting pipe one end with first cylinder body left end intercommunication, the fifth connecting pipe other end with the fourth connecting pipe is close to the one end intercommunication of decomposition enzyme liquid case, the fourth connecting pipe is close to one of first connecting pipe is served and is provided with the ball valve, the pivot is kept away from the one end of cam extends to outside the barrel, and with the valve rod fixed connection of ball valve.
Preferably, a liquid inlet pipe is arranged at the upper end of the enzyme decomposition liquid box, a sealing cover is arranged at the upper end of the liquid inlet pipe, and the liquid inlet pipe is in threaded connection with the sealing cover.
Preferably, the second connecting pipe, the third connecting pipe and the fourth connecting pipe are all provided with valves.
Preferably, a groove body is arranged on the side wall of the feeding box, a processor is arranged in the groove body, a pH value sensor is arranged on the inner wall of the feeding box and is electrically connected with the processor, an alarm is arranged on the outer wall of the groove body, the pH value sensor is electrically connected with the processor, and the processor is electrically connected with the alarm;
be provided with control circuit in the treater, control circuit includes: a resistor R1, a resistor R2, a resistor R3, a resistor R4, a resistor R5, a resistor R6, a resistor R7, an adjustable resistor RP, a diode D1, a zener diode ZD1, an operational amplifier U1, an operational amplifier U2, an operational amplifier U3, a capacitor C1, a capacitor C2, a capacitor C3 and a transistor Q;
the output end of the PH sensor is connected to one end of the adjustable resistor RP and the anode of the zener diode ZD1, the other end of the adjustable resistor RP is connected to the anode of the diode D1, the cathode of the diode D1 is connected to one end of the resistor R3, the other end of the resistor R3 is connected to the positive input end of the operational amplifier U1, the inverting input end of the operational amplifier U1 is connected to one end of the resistor R4, the other end of the resistor R4 is connected to the positive input end of the operational amplifier U2, the cathode of the zener diode ZD1 is connected to one end of the resistor R1, the other end of the resistor R1 is connected to one end of the resistor R2, the other end of the resistor R2 is connected to the inverting input end of the operational amplifier U8, the two ends of the resistor R1 are connected in parallel to the capacitor C1, and the two ends of the resistor R4 are connected in parallel to the capacitor;
the signal output end of the operational amplifier U1 is connected with the collector end of the transistor Q, the base end of the transistor Q is connected with one end of the resistor R5, the other end of the resistor R5 is connected with the positive phase input end of the operational amplifier U2, the emitter end of the transistor Q is connected with the positive phase input end of the operational amplifier U3, the signal output end of the operational amplifier U2 is connected with one end of the resistor R6, the other end of the resistor R6 is connected with one end of the resistor R7, the other end of the resistor R7 is electrically connected with the negative phase input end of the operational amplifier U3, the two ends of the resistor R7 are connected in parallel with the capacitor C3, and the signal output end of the operational amplifier U3 is electrically connected with the input end of the alarm.
The technical scheme of the invention has the following advantages: the invention relates to a device for preparing anti-aging small molecular peptides, which comprises the following components: the box, the box upper end is provided with the opening, be provided with the feed box near the opening part in the box, feed box outer wall with the box inner wall passes through the bracing piece and connects, the below of feed box is provided with the agitator tank, first connecting pipe one end with feed box bottom intercommunication, the first connecting pipe other end with agitator tank top intercommunication, agitator tank bottom is provided with row material pipe, the below of arranging the material pipe is provided with the filter screen, the filter screen below is provided with adsorptive separation debitterizing ware, adsorptive separation debitterizing ware's export below is provided with freeze dryer, freeze dryer's export and the feed inlet intercommunication of sterilization case. Because the raw materials, the acid-base solution and the lytic enzyme are fully stirred and mixed uniformly in the stirring box, the reaction speed is accelerated, the reaction is more sufficient, and the extraction rate of the small molecular peptide is greatly improved.
Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and drawings.
The technical solution of the present invention is further described in detail by the accompanying drawings and embodiments.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic structural diagram of the present invention.
FIG. 2 is a schematic view showing the internal structure of the agitation tank of the present invention.
FIG. 3 is a schematic structural diagram of a solution preparing apparatus according to the present invention.
Fig. 4 is a schematic view of the internal structure of the cartridge according to the present invention.
Fig. 5 is a schematic structural view of the cam in the present invention.
FIG. 6 is a schematic structural diagram of the detecting device of the present invention.
FIG. 7 is a schematic diagram of a control circuit according to the present invention.
The drawings are numbered as follows: 1-box body, 2-feeding box, 3-supporting rod, 4-stirring box, 5-first connecting pipe, 6-discharging pipe, 7-filtering net, 8-adsorption separation debitterizing device, 9-freeze dryer, 10-sterilizing box, 11-waste discharging pipe, 12-groove body, 13-first motor, 14-output shaft, 15-rotating shaft, 16-stirring blade, 17-sealing cover, 18-supporting leg, 19-universal wheel, 20-cylinder body, 21-first cylinder body, 22-second cylinder body, 23-first connecting pipe, 24-first piston, 25-second piston, 26-fixing plate, 27-through hole, 28-moving column, 29-flat plate, 30-spring and 31-second motor, 32-rotating shaft, 33-cam, 34-second connecting pipe, 35-alkaline liquid tank, 36-third connecting pipe, 37-acid liquid tank, 38-fourth connecting pipe, 39-decomposition enzyme liquid tank, 40-fifth connecting pipe, 41-ball valve, 42-valve rod, 43-liquid inlet pipe, 44-sealing cover, 45-groove body, 46-processor, 47-pH value sensor and 48-alarm.
Detailed Description
The preferred embodiments of the present invention will be described in conjunction with the accompanying drawings, and it will be understood that they are described herein for the purpose of illustration and explanation and not limitation.
The embodiment of the invention provides a device for preparing anti-aging small molecule peptide, as shown in figure 1, comprising: box 1, 1 upper end of box is provided with the opening, be provided with feed box 2 near the opening part in the box 1, 2 outer walls of feed box with 1 inner wall of box passes through bracing piece 3 to be connected, the below of feed box 2 is provided with agitator tank 4, 5 one end of first connecting pipe with 2 bottom intercommunications of feed box, the 5 other ends of first connecting pipe with 4 top intercommunications of agitator tank, 4 bottoms of agitator tank are provided with row material pipe 6, the below of arranging material pipe 6 is provided with filter screen 7, 7 below of filter screen are provided with adsorptive separation and take off bitter ware 8, adsorptive separation takes off bitter ware 8's export below and is provided with freeze dryer 9, freeze dryer 9's export and sterilization case 10's feed inlet intercommunication.
The working principle and the beneficial technical effects of the technical scheme are as follows: crushing pea, sea buckthorn and other plant material, feeding into the feeding box 2 via the opening in the box 1, adding acid, alkali solution and decomposing enzyme, mixing via the first connecting pipe 5 in the stirring box 4, filtering via the filter net 7, separating and debittering via the adsorption separation debittering device 8, freeze drying via the freeze drier 9, and sterilizing in the sterilizing box 10. Because the raw materials, the acid-base solution and the lytic enzyme are fully stirred and mixed uniformly in the stirring box 4, the reaction speed is accelerated, the reaction is more sufficient, and the extraction rate of the small molecular peptide is greatly improved.
In one embodiment, the side wall of said adsorptive separation debitterizing device 8 is provided with a waste discharge pipe 11.
The working principle and the beneficial technical effects of the technical scheme are as follows: the waste material after the reaction of the adsorption separation debitterizing device 8 is discharged from a waste material discharge pipe 11.
In one embodiment, as shown in fig. 2, a groove body 12 is disposed on an inner wall of the stirring box 4, a first motor 13 is disposed in the groove body 12, an output shaft 14 of the first motor 13 is disposed in a horizontal direction, one end of the output shaft 14 extends into the stirring box 4 and is connected to a rotating shaft 15 in the horizontal direction, and a plurality of stirring blades 16 are disposed on an outer wall of the rotating shaft 15 along a circumferential direction.
The working principle and the beneficial technical effects of the technical scheme are as follows: the electric output shaft 14 of the first motor 13 rotates, the output shaft 14 drives the rotating shaft 15 and the stirring blades 16 to rotate at a high speed, and the material machine liquid in the stirring box 4 is stirred at a high speed so as to be uniformly mixed.
In one embodiment, the top end of the case 1 is provided with a sealing cover 17.
The working principle and the beneficial technical effects of the technical scheme are as follows: the sealing cover 17 can prevent dust or foreign matters from entering the box body 1 to influence the extraction purity of the small molecule peptide.
In one embodiment, the bottom end of the housing 1 is provided with support legs 18.
The working principle and the beneficial technical effects of the technical scheme are as follows: the support legs 18 function to support the case 1.
In one embodiment, the bottom end of the support leg 18 is provided with a universal wheel 19.
The working principle and the beneficial technical effects of the technical scheme are as follows: the universal wheels 19 facilitate the carrying of the box body.
In one embodiment, as shown in fig. 3-5, further comprising a solution preparing device, the solution preparing device comprising:
the feeding box comprises a barrel 20, the barrel 20 is arranged in the vertical direction, a first cylinder 21 and a second cylinder 22 are respectively arranged at the upper end and the lower end of the barrel 20, one end of a first connecting pipe 23 is communicated with the side wall of the feeding box 2, and the other end of the first connecting pipe 23 is communicated with the left end of the second cylinder 22;
a first piston 24 provided in the first cylinder 21, the first piston 24 being capable of reciprocating up and down in the first cylinder 21;
a second piston 25 disposed in the second cylinder 22, the second piston 25 being capable of reciprocating up and down in the second cylinder 22;
the fixing plate 26 is horizontally arranged on the inner wall of the cylinder 20, a through hole 27 is formed in the fixing plate 26, a moving column 28 is arranged in the through hole 27, the moving column 28 can reciprocate up and down along the through hole 27, one end of the moving column 28 extends into the first cylinder 21 and is fixedly connected with the first piston 24, and the other end of the moving column 28 extends into the second cylinder 22 and is fixedly connected with the second piston 25;
the movable column 28 penetrates through the flat plate 29 and is fixedly connected with the flat plate 29, a spring 30 is sleeved on the movable column 28, one end of the spring 30 is fixedly connected with the flat plate 29, and the other end of the spring 30 is fixedly connected with the fixed plate 26;
the second motor 31 is arranged at the top end of the fixing plate 26, an output shaft of the second motor 31 is connected with one end of a rotating shaft 32, a cam 33 is arranged at the other end of the rotating shaft 32, and the top end of the cam 33 is contacted with the bottom end of the flat plate 29;
one end of the second connecting pipe 34 is communicated with the right end of the second cylinder 22, and the other end of the second connecting pipe 34 is communicated with an alkaline liquid tank 35;
one end of the third connecting pipe 36 is communicated with the right end of the first cylinder 21, and the other end of the third connecting pipe 36 is communicated with an acid liquor tank 37;
one end of the fourth connecting pipe 38 is communicated with the middle of the first connecting pipe 23, and the other end of the fourth connecting pipe 38 is communicated with a decomposition enzyme liquid tank 39;
one end of the fifth connecting pipe 40 is communicated with the left end of the first cylinder 21, the other end of the fifth connecting pipe 40 is communicated with one end of the fourth connecting pipe 38 close to the liquid decomposing enzyme tank 39, a ball valve 41 is arranged at one end of the fourth connecting pipe 38 close to the first connecting pipe 23, one end of the rotating shaft 32 far away from the cam 33 extends out of the cylinder 20 and is fixedly connected with a valve rod 42 of the ball valve 41.
The working principle and the beneficial technical effects of the technical scheme are as follows: the alkali liquor in the alkali liquor tank 35 flows into the second cylinder 22 through the second connecting pipe 34, the acid liquor in the acid liquor tank 37 flows into the first cylinder 21 through the third connecting pipe 36, the decomposing enzyme in the decomposing enzyme liquor tank 39 flows into the fourth connecting pipe 38, when the solution adjustment is needed, the second motor 31 at the top end of the fixed plate 26 is started, the second motor 31 drives the rotating shaft 32 and the cam 33 to rotate, the flat plate 29 is tightly attached to the cam 33 due to the pulling force of the spring 30, the flat plate 29 reciprocates up and down in the cylinder 20, the flat plate 29 drives the moving column 28 to reciprocate up and down along the through hole 27, the upper end and the lower end of the moving column 28 respectively drive the first piston 24 to reciprocate up and down in the first cylinder 21, the second piston 25 reciprocates up and down in the second cylinder 22, the intermittent switch closing of the first cylinder 21 and the second cylinder 22 is realized, and simultaneously, the rotating shaft 32 rotates, the valve rod 42 is driven to rotate, the valve rod 42 rotates, and the ball valve 41 is intermittently opened or closed, so that the flow of the liquid entering the fourth connecting pipe 38, the fifth connecting pipe 40 and the first connecting pipe 23 is adjusted, and the adjusted solution enters the feeding box 2 to react with the material. The linkage of ball valve 41, first piston 24 and second piston 25 just can be realized to any angle of rotation of second motor 31, will correspond the allotment proportion of three kinds of liquid to realize the pH value of solution and the allotment of decomposition enzyme, compare in the manual work and carry out the solution allotment, the allotment precision is higher, and the allotment speed is also faster, realizes the automation of solution allotment.
In one embodiment, the upper end of the liquid tank 39 is provided with a liquid inlet pipe 43, the upper end of the liquid inlet pipe 43 is provided with a sealing cover 44, and the liquid inlet pipe 43 is in threaded connection with the sealing cover 44.
The working principle and the beneficial technical effects of the technical scheme are as follows: when the decomposition enzyme in the decomposition enzyme liquid tank 39 is consumed, the sealing cover 44 is opened, and new decomposition enzyme is added through the liquid inlet pipe 43.
In one embodiment, the second connecting pipe 34, the third connecting pipe 36 and the fourth connecting pipe 38 are provided with valves.
The working principle and the beneficial technical effects of the technical scheme are as follows: the valves can open or close the second connection pipe 34, the third connection pipe 36 and the fourth connection pipe 38.
In one embodiment, as shown in fig. 6 to 7, a groove body 45 is provided on the side wall of the feeding box 2, a processor 46 is provided in the groove body 45, a PH sensor 47 is provided on the inner wall of the feeding box 2, the PH sensor 44 is electrically connected with the processor 46, an alarm 48 is provided on the outer wall of the groove body 45, the PH sensor 47 is electrically connected with the processor 46, and the processor 46 is electrically connected with the alarm 48;
a control circuit is disposed within the processor 46, the control circuit including: a resistor R1, a resistor R2, a resistor R3, a resistor R4, a resistor R5, a resistor R6, a resistor R7, an adjustable resistor RP, a diode D1, a zener diode ZD1, an operational amplifier U1, an operational amplifier U2, an operational amplifier U3, a capacitor C1, a capacitor C2, a capacitor C3 and a transistor Q;
the output end of the PH sensor 47 is respectively connected with one end of the adjustable resistor RP and the anode of the zener diode ZD1, the other end of the adjustable resistor RP is connected with the anode of the diode D1, the cathode of the diode D1 is connected with one end of the resistor R3, the other end of the resistor R3 is connected with the non-inverting input end of the operational amplifier U1, the inverting input end of the operational amplifier U1 is connected with one end of the resistor R4, the other end of the resistor R4 is connected with the non-inverting input end of the operational amplifier U2, the cathode of the zener diode ZD1 is connected with one end of the resistor R1, the other end of the resistor R1 is connected with one end of the resistor R2, the other end of the resistor R2 is connected with the inverting input end of the operational amplifier U2, the capacitor C1 is connected in parallel with two ends of the resistor R1, and the capacitor C2 is connected in parallel with two ends of the resistor R4;
the signal output end of the operational amplifier U1 is connected with the collector end of the transistor Q, the base end of the transistor Q is connected with one end of the resistor R5, the other end of the resistor R5 is connected with the positive phase input end of the operational amplifier U2, the emitter end of the transistor Q is connected with the positive phase input end of the operational amplifier U3, the signal output end of the operational amplifier U2 is connected with one end of the resistor R6, the other end of the resistor R6 is connected with one end of the resistor R7, the other end of the resistor R7 is electrically connected with the negative phase input end of the operational amplifier U3, the two ends of the resistor R7 are connected in parallel with the capacitor C3, and the signal output end of the operational amplifier U3 is electrically connected with the input end of the alarm 48.
The working principle and the beneficial technical effects of the technical scheme are as follows: the data detected by the PH value sensor 44 is amplified in multiple stages through the operational amplifier U1, the operational amplifier U2 and the operational amplifier U3, the detection precision is higher and more sensitive, and the voltage stabilizing diode Dz can prevent the voltage fluctuation, so that the control circuit is more stable.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.

Claims (10)

1. An apparatus for preparing an anti-aging small molecule peptide, comprising: the device comprises a box body (1), an opening is arranged at the upper end of the box body (1), a feeding box (2) is arranged in the box body (1) and close to the opening, the outer wall of the feeding box (2) is connected with the inner wall of the box body (1) through a support rod (3), a stirring box (4) is arranged below the feeding box (2), one end of a first connecting pipe (5) is communicated with the bottom of the feeding box (2), the other end of the first connecting pipe (5) is communicated with the top end of the stirring box (4), a material discharging pipe (6) is arranged at the bottom end of the stirring box (4), a filter screen (7) is arranged below the material discharging pipe (6), an adsorption separation debitterizing device (8) is arranged below the filter screen (7), a freeze dryer (9) is arranged below the outlet of the adsorption separation debitterizing device (8), the outlet of the freeze dryer (9) is communicated with the feed inlet of the sterilizing box (10).
2. The apparatus for preparing anti-aging small molecule peptide according to claim 1, wherein a waste discharge pipe (11) is disposed on the side wall of the adsorption separation debittering device (8).
3. The device for preparing anti-aging small molecular peptide according to claim 1, wherein a groove body (12) is arranged on the inner wall of the stirring box (4), a first motor (13) is arranged in the groove body (12), an output shaft (14) of the first motor (13) is horizontally arranged, one end of the output shaft (14) extends into the stirring box (4) and is connected with a rotating shaft (15) in the horizontal direction, and a plurality of stirring blades (16) are circumferentially arranged on the outer wall of the rotating shaft (15).
4. The apparatus for preparing anti-aging small molecule peptide according to claim 1, wherein the top end of the case (1) is provided with a sealing cover (17).
5. The apparatus for preparing anti-aging small molecule peptide according to claim 1, wherein the bottom end of the box body (1) is provided with a supporting leg (18).
6. The apparatus for preparing anti-aging small molecule peptide according to claim 5, wherein the bottom end of the supporting leg (18) is provided with a universal wheel (19).
7. The apparatus for preparing anti-aging small molecule peptide according to claim 1, further comprising a solution preparing apparatus, wherein the solution preparing apparatus comprises:
the feeding box comprises a barrel (20), wherein the barrel (20) is arranged in the vertical direction, a first cylinder body (21) and a second cylinder body (22) are respectively arranged at the upper end and the lower end of the barrel (20), one end of a first connecting pipe (23) is communicated with the side wall of the feeding box (2), and the other end of the first connecting pipe (23) is communicated with the left end of the second cylinder body (22);
a first piston (24) disposed in the first cylinder (21), the first piston (24) being capable of reciprocating up and down in the first cylinder (21);
a second piston (25) disposed in the second cylinder (22), the second piston (25) being capable of reciprocating up and down in the second cylinder (22);
the fixing plate (26) is horizontally arranged on the inner wall of the cylinder body (20), a through hole (27) is formed in the fixing plate (26), a moving column (28) is arranged in the through hole (27), the moving column (28) can reciprocate up and down along the through hole (27), one end of the moving column (28) extends into the first cylinder body (21) and is fixedly connected with the first piston (24), and the other end of the moving column (28) extends into the second cylinder body (22) and is fixedly connected with the second piston (25);
the movable column (28) penetrates through the flat plate (29) and is fixedly connected with the flat plate (29), a spring (30) is sleeved on the movable column (28), one end of the spring (30) is fixedly connected with the flat plate (29), and the other end of the spring (30) is fixedly connected with the fixed plate (26);
the second motor (31) is arranged at the top end of the fixing plate (26), an output shaft of the second motor (31) is connected with one end of a rotating shaft (32), a cam (33) is arranged at the other end of the rotating shaft (32), and the top end of the cam (33) is in contact with the bottom end of the flat plate (29);
one end of the second connecting pipe (34) is communicated with the right end of the second cylinder body (22), and the other end of the second connecting pipe (34) is communicated with an alkaline liquid tank (35);
one end of the third connecting pipe (36) is communicated with the right end of the first cylinder body (21), and the other end of the third connecting pipe (36) is communicated with an acid liquid tank (37);
one end of the fourth connecting pipe (38) is communicated with the middle of the first connecting pipe (23), and the other end of the fourth connecting pipe (38) is communicated with a decomposing enzyme liquid tank (39);
fifth connecting pipe (40), fifth connecting pipe (40) one end with first cylinder body (21) left end intercommunication, the fifth connecting pipe (40) other end with fourth connecting pipe (38) are close to the one end intercommunication of decomposition enzyme liquid case (39), fourth connecting pipe (38) are close to one of first connecting pipe (23) is served and is provided with ball valve (41), pivot (32) are kept away from the one end of cam (33) extends to outside barrel (20), and with valve rod (42) fixed connection of ball valve (41).
8. The apparatus for preparing anti-aging small molecule peptide as claimed in claim 7, wherein the upper end of the decomposition enzyme liquid tank (39) is provided with a liquid inlet pipe (43), the upper end of the liquid inlet pipe (43) is provided with a sealing cover (44), and the liquid inlet pipe (43) is in threaded connection with the sealing cover (44).
9. The apparatus for preparing anti-aging small molecule peptide according to claim 7, wherein valves are disposed on the second connecting tube (34), the third connecting tube (36) and the fourth connecting tube (38).
10. The device for preparing the anti-aging small molecule peptide according to claim 1, wherein a groove body (45) is arranged on the side wall of the feeding box (2), a processor (46) is arranged in the groove body (45), a pH value sensor (47) is arranged on the inner wall of the feeding box (2), the pH value sensor (44) is electrically connected with the processor (46), an alarm (48) is arranged on the outer wall of the groove body (45), the pH value sensor (47) is electrically connected with the processor (46), and the processor (46) is electrically connected with the alarm (48);
a control circuit is disposed within the processor (46), the control circuit comprising: a resistor R1, a resistor R2, a resistor R3, a resistor R4, a resistor R5, a resistor R6, a resistor R7, an adjustable resistor RP, a diode D1, a zener diode ZD1, an operational amplifier U1, an operational amplifier U2, an operational amplifier U3, a capacitor C1, a capacitor C2, a capacitor C3 and a transistor Q;
the output end of the PH value sensor (47) is respectively connected with one end of the adjustable resistor RP and the anode of the zener diode ZD1, the other end of the adjustable resistor RP is connected with the anode of the diode D1, the cathode of the diode D1 is connected with one end of the resistor R3, the other end of the resistor R3 is connected with the non-inverting input end of the operational amplifier U1, the inverting input end of the operational amplifier U1 is connected with one end of the resistor R4, the other end of the resistor R4 is connected with the non-inverting input end of the operational amplifier U2, the cathode of the zener diode ZD1 is connected with one end of the resistor R1, the other end of the resistor R1 is connected with one end of the resistor R2, the other end of the resistor R2 is connected with the inverting input end of the operational amplifier U2, the capacitor C1 is connected in parallel with two ends of the resistor R1, and the capacitor C2 is connected in parallel with two ends of the resistor R4;
the signal output end of the operational amplifier U1 is connected with the collector end of the transistor Q, the base end of the transistor Q is connected with one end of the resistor R5, the other end of the resistor R5 is connected with the positive phase input end of the operational amplifier U2, the emitter end of the transistor Q is connected with the positive phase input end of the operational amplifier U3, the signal output end of the operational amplifier U2 is connected with one end of the resistor R6, the other end of the resistor R6 is connected with one end of the resistor R7, the other end of the resistor R7 is electrically connected with the negative phase input end of the operational amplifier U3, the two ends of the resistor R7 are connected in parallel with the capacitor C3, and the signal output end of the operational amplifier U3 is electrically connected with the input end of the alarm (48).
CN202010031992.XA 2020-01-13 2020-01-13 Device for preparing anti-aging small molecular peptide Active CN111197005B (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN205874445U (en) * 2016-07-15 2017-01-11 安徽精准医疗产业研究院有限公司 Generate micro molecule peptide equipment
CN207793246U (en) * 2018-01-19 2018-08-31 哈尔滨商业大学 A kind of device preparing pea small-molecular peptides
US20190022590A1 (en) * 2017-07-21 2019-01-24 Titan Biological & Agricultural Technology Co., Ltd. Device for separation and purification of collagen type 2 in chicken bones
CN110605085A (en) * 2019-09-11 2019-12-24 陕西羊老大服饰股份有限公司 Purification equipment for wool and soybean protein fibers

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN205874445U (en) * 2016-07-15 2017-01-11 安徽精准医疗产业研究院有限公司 Generate micro molecule peptide equipment
US20190022590A1 (en) * 2017-07-21 2019-01-24 Titan Biological & Agricultural Technology Co., Ltd. Device for separation and purification of collagen type 2 in chicken bones
CN207793246U (en) * 2018-01-19 2018-08-31 哈尔滨商业大学 A kind of device preparing pea small-molecular peptides
CN110605085A (en) * 2019-09-11 2019-12-24 陕西羊老大服饰股份有限公司 Purification equipment for wool and soybean protein fibers

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