CN215609488U

CN215609488U - Electromagnetic device for purifying large-volume protein

Info

Publication number: CN215609488U
Application number: CN202122162112.5U
Authority: CN
Inventors: 胡美丽
Original assignee: Genfine Biotech Beijing Co ltd
Current assignee: Genfine Biotech Beijing Co ltd
Priority date: 2021-09-08
Filing date: 2021-09-08
Publication date: 2022-01-25
Anticipated expiration: 2031-09-08

Abstract

The utility model discloses an electromagnetic device for purifying large-volume protein, which comprises: the tank comprises a tank body, a feed inlet arranged at the top of the tank body, a temperature control mechanism and a stirring mechanism which are respectively arranged on the tank body, wherein an electromagnetic coil is arranged outside the tank body; the temperature control mechanism comprises a refrigerant circulating machine, the refrigerant circulating machine is mounted outside the tank body, a water outlet of the refrigerant circulating machine is communicated with a refrigerant pipe, and the refrigerant pipe is concavely arranged outside the tank body; the protein sample and the matched and corresponding protein-purified magnetic beads are thrown into the tank through the feed inlet, then the refrigerant machine is started, so that the protein sample is kept at a low temperature, the activity of the protein is ensured, the matched and coupled protein magnetic beads are arranged through the electromagnetic coil, the target protein can be efficiently separated, the operation is simple and efficient, the electromagnetic coil and the stirring mechanism are matched for use, the protein sample and the protein magnetic beads can be ensured to fully react, and the protein purification efficiency is ensured.

Description

Electromagnetic device for purifying large-volume protein

Technical Field

The utility model relates to the technical field of biological experiments, in particular to an electromagnetic device for purifying large-volume protein.

Background

The separation and purification of proteins is widely used in biochemical research applications and is an important operation technology. To study a protein, the protein must first be purified from other proteins and non-protein molecules. The problems of protein purification research and development and pilot scale amplification exist in the field of biological pharmacy, hundreds of tons of protein fillers are needed for industrial-grade large-volume protein purification, the application of the resin chromatography principle is more, but the chromatography principle has a series of restriction factors of overlarge chromatography column, overlong chromatography time and the like, so that the production efficiency is low, and therefore, the electromagnetic device for large-volume protein purification is provided.

SUMMERY OF THE UTILITY MODEL

The present invention aims to provide an electromagnetic device for large volume protein purification to solve the problems set forth in the background art.

In order to achieve the purpose, the utility model provides the following technical scheme: an electromagnetic device for large volume protein purification, comprising: the tank comprises a tank body, a feed inlet arranged at the top of the tank body, a temperature control mechanism and a stirring mechanism which are respectively arranged on the tank body, wherein an electromagnetic coil is arranged outside the tank body;

the temperature control mechanism comprises a circulating refrigerant machine, the circulating refrigerant machine is installed outside the tank body, a water outlet of the circulating refrigerant machine is communicated with a refrigerant pipe, the refrigerant pipe is arranged outside the tank body in a concave shape, and the other end of the refrigerant pipe is communicated with a water inlet of the circulating refrigerant machine.

Through adopting above-mentioned technical scheme, throw the inside of jar body into with protein sample and collocation corresponding protein purification's magnetic bead through the feed inlet, then start the refrigerant machine for protein sample keeps the low temperature, thereby guarantees the activity of albumen, and sets up collocation coupling affinity's protein magnetic bead through solenoid, can the efficient isolate destination protein, and is simple high-efficient, uses through solenoid and rabbling mechanism cooperation, can guarantee that protein sample and protein magnetic bead fully react, guarantees protein purification efficiency.

Preferably, the bottom of the tank body is communicated with a discharge pipe.

By adopting the technical scheme, the purified protein material is conveniently discharged.

Preferably, a discharge valve is installed outside the discharge pipe.

Through adopting above-mentioned technical scheme for discharge the discharge of material pipe and control.

Preferably, the feeding port is internally communicated with a feeding pipe.

By adopting the technical scheme, the scattering of the protein sample and the protein magnetic beads in the putting process is avoided.

Preferably, the stirring mechanism comprises a motor, an output shaft of the motor penetrates through the top of the tank body and is fixedly connected with an upper stirring rod, and the bottom of the upper stirring rod is fixedly connected with a lower stirring rod.

By adopting the technical scheme, the protein sample and the protein magnetic beads are stirred.

Preferably, the lower stirring rod is arc-shaped.

Through adopting above-mentioned technical scheme, promote the effect to protein sample and protein magnetic bead stirring.

Compared with the prior art, the utility model has the beneficial effects that:

this scheme is put the inside of jar body through the feed inlet with protein sample and the corresponding protein purification's of collocation magnetic bead, then starts the refrigerant machine for protein sample keeps the low temperature, thereby guarantees the activity of albumen, and sets up the compatible protein magnetic bead of collocation coupling through solenoid, can the efficient isolate target protein, and is simple high-efficient, uses through solenoid and rabbling mechanism cooperation, can guarantee protein sample and protein magnetic bead fully react, guarantees protein purification efficiency.

Drawings

FIG. 1 is a front view of an electromagnetic apparatus for bulk protein purification according to the present invention in use;

FIG. 2 is a schematic diagram of the internal cross-sectional structure of an electromagnetic apparatus for large-volume protein purification according to the present invention in use;

FIG. 3 is a schematic view of the connection structure of the upper stirring rod and the lower stirring rod of the electromagnetic device for purifying large-volume protein according to the present invention.

In the figure: 1. a tank body; 10. a feed inlet; 11. a feed pipe; 12. a discharge pipe; 13. a discharge valve; 14. an electromagnetic coil; 2. a temperature control mechanism; 20. a refrigerant circulating machine; 21. a refrigerant pipe; 3. a stirring mechanism; 30. a motor; 31. an upper stirring rod; 32. a lower stirring rod.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-3, the present invention provides a technical solution:

an electromagnetic device for large volume protein purification, comprising: jar body 1 and the feed inlet 10 of seting up in jar body 1 top, in order to avoid the in-process scattering that albumen sample and albumen magnetic bead put in, the inside intercommunication of feed inlet 10 has inlet pipe 11.

The temperature control mechanism 2 and the stirring mechanism 3 are arranged on the tank body 1, the electromagnetic coil 14 is arranged outside the tank body 1, a discharge pipe 12 is arranged for facilitating discharge of purified protein materials, and the discharge pipe 12 is communicated with the bottom of the tank body 1; for controlling the discharge of the discharge pipe 12, a discharge valve 13 is provided, the discharge valve 13 being installed outside the discharge pipe 12.

Referring to fig. 1 to 3, the temperature control mechanism 2 is composed of: the temperature control mechanism 2 comprises a circulating refrigerant machine 20 arranged outside the tank body 1, a refrigerant pipe 21 is communicated with a water outlet of the circulating refrigerant machine 20, the refrigerant pipe 21 is arranged outside the tank body 1 in a concave shape, and the other end of the refrigerant pipe 21 is communicated with a water inlet of the circulating refrigerant machine 20.

The control principle of the temperature control mechanism 2 is as follows: after the protein sample and the protein magnetic beads enter the tank body 1, the circulating refrigerant machine 20 is started, and the low-temperature operation is kept during the protein purification operation through the refrigerant pipe 21, so that the activity of the protein is ensured.

Referring to fig. 1 to 3, the stirring composition of the stirring mechanism 3: the stirring mechanism 3 comprises a motor 30, an output shaft of the motor 30 penetrates through the top of the tank body 1, an upper stirring rod 31 is installed at the bottom of the output shaft of the motor 30 through a coupler, and the bottom of the upper stirring rod 31 is fixedly connected with a lower stirring rod 32.

Stirring principle of the stirring mechanism 3: the starter motor 30, the output shaft of motor 30 drives and goes up puddler 31 and rotate, makes puddler 32 follow puddler 31 and rotate down simultaneously to the realization is to the effect of albumen sample and albumen magnetic bead stirring.

In order to improve the stirring effect of the protein sample and the protein magnetic beads, the lower stirring rod 32 is arc-shaped.

The working principle is as follows: when in use, the protein sample and the protein magnetic bead stirring and feeding pipe 11 and the feeding hole 10 are put into the tank body 1, then, a switch group and a circulating refrigerant machine 20 are started (the switch group is arranged outside the tank body 1 and comprises a switch button for controlling the motor 30, the electromagnetic coil 14 and the discharge valve 13), so that the discharge valve 13 is closed, the electromagnetic coil 14 and the motor 30 are started, an output shaft of the motor 30 drives the upper stirring rod 31 to rotate, and simultaneously, the lower stirring rod 32 rotates along with the upper stirring rod 31, thereby realizing the effect of stirring the protein sample and the protein magnetic beads, when the protein sample and the protein magnetic beads are stirred, the electromagnetic coil 14 generates magnetic force to make the tank body 1 attached with magnetic force, thereby adsorbing the magnetic beads, and ensuring that the protein sample and the protein magnetic beads are fully reacted by matching the electromagnetic coil 14 with the stirring mechanism 3; after the protein sample and the protein magnetic beads enter the tank body 1, the protein sample and the protein magnetic beads are kept operating at a low temperature during purification operation through the combined use of the circulating refrigerant machine 20 and the refrigerant pipe 21, so that the activity of the protein is ensured.

The parts not involved in the present invention are the same as or can be implemented by the prior art. Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. An electromagnetic device for large volume protein purification, comprising: jar body (1), set up in feed inlet (10) at the top of the jar body (1), install temperature control mechanism (2) and rabbling mechanism (3) on jar body (1) respectively, its characterized in that: an electromagnetic coil (14) is arranged outside the tank body (1);

the temperature control mechanism (2) comprises a circulating refrigerant machine (20), the circulating refrigerant machine (20) is installed outside the tank body (1), a water outlet of the circulating refrigerant machine (20) is communicated with a refrigerant pipe (21), the refrigerant pipe (21) is arranged outside the tank body (1) in a concave shape, and the other end of the refrigerant pipe (21) is communicated with a water inlet of the circulating refrigerant machine (20).

2. An electromagnetic device for large volume protein purification according to claim 1, characterized in that: the bottom of the tank body (1) is communicated with a discharge pipe (12).

3. An electromagnetic device for large volume protein purification according to claim 2, characterized in that: and a discharge valve (13) is arranged outside the discharge pipe (12).

4. An electromagnetic device for large volume protein purification according to claim 1, characterized in that: the feeding hole (10) is communicated with a feeding pipe (11).

5. An electromagnetic device for large volume protein purification according to claim 1, characterized in that: rabbling mechanism (3) include motor (30), the output shaft of motor (30) run through in puddler (31) on the top and the fixedly connected with of jar body (1), puddler (32) under the bottom fixedly connected with of going up puddler (31).

6. An electromagnetic device for large volume protein purification according to claim 5, characterized in that: the lower stirring rod (32) is arc-shaped.