CN206109412U - Biological enzymolysis preprocessing device - Google Patents
Biological enzymolysis preprocessing device Download PDFInfo
- Publication number
- CN206109412U CN206109412U CN201620752792.2U CN201620752792U CN206109412U CN 206109412 U CN206109412 U CN 206109412U CN 201620752792 U CN201620752792 U CN 201620752792U CN 206109412 U CN206109412 U CN 206109412U
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- CN
- China
- Prior art keywords
- disintegrating machine
- rotating shaft
- push
- inner shell
- biological enzymolysis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
The utility model provides a biological enzymolysis preprocessing device, including breaker shell (11) and breaker inner shell (12), intercommunication feed inlet (13) of breaker inner shell (12), be equipped with broken mechanism (14) of grinding the raw materials in breaker inner shell (12). This utility model biological enzymolysis preprocessing device simple structure, manipulate convenient, can improve enzymolysis efficiency, the security is good, has better application prospect.
Description
Technical field
This utility model belongs to zymolysis technique field, more particularly, it relates to a kind of biological enzymolysis pretreatment unit.
Background technology
Traditional material extracting mode is adopted and stays the modes such as powder, water extraction or low-temperature homogenate mostly, or this kind of mode is raw
Thing availability is low, or side reaction is more.It is industrial at present mainly HVP to be produced using acid-hydrolysis method.But acid hydrolytic reaction condition swashs
It is strong, aminoacid can be destroyed, additionally, acid-hydrolysis method can produce carcinogen；Also enzyme hydrolysis method is adopted, but enzymolysis equipment is single, enzyme
Solution inefficiency, it is impossible to meet the needs of people.
Utility model content
The purpose of this utility model is to solve the problems, such as prior art, there is provided a kind of simple structure, can improve enzymolysis
The biological enzymolysis pretreatment unit of efficiency.
To achieve these goals, the technical scheme that this utility model is taken is：A kind of biological enzymolysis pretreatment unit, bag
Include disintegrating machine shell, disintegrating machine inner shell and connect the feed(raw material)inlet of the disintegrating machine inner shell, be provided with brokenly in the disintegrating machine inner shell
The crushing mechanism of breakdown mill raw material.
To make above-mentioned technical proposal more detailed and concrete, this utility model also provides optimization technique further below
Scheme, to obtain satisfied practical function：
The crushing mechanism includes broken motor, and the outfan connection disintegrating machine rotating shaft of the broken motor is described broken
Machine rotating shaft is provided with broken screw feeder blade.
The disintegrating machine rotating shaft is extended straight down by the upper end of the disintegrating machine inner shell, the lower end of the disintegrating machine rotating shaft
Be provided with reclaiming plate, the reclaiming plate be provided with it is some go out material through hole.
The breaker outside is provided with ptyalin storage tank, and ptyalin is via ptyalin delivery conduit
It is delivered in the disintegrating machine inner shell.
The ptyalin delivery conduit is the vertical high-pressure delivery pipe for extending, in the ptyalin delivery conduit
Side wall is provided with some spray orifices.
The disintegrating machine outer casing bottom of the breaker is provided with and for fragment outwards to export pushing away to next reflection device
Send device.
The pusher include horizontal cross arrangement push rotating shaft, located at it is described push rotating shaft on push blade and
Drive the push motor for pushing axis of rotation work.
One end that the disintegrating machine shell is stretched out in the push rotating shaft is provided with discharge gate, the discharge gate and the first order
The charging aperture connection of enzymolysis device.The discharge gate is the pyramidal structure tapered into along push direction section.
This utility model compared with prior art, with advantages below：This utility model biological enzymolysis pretreatment unit, can
Stirring pretreatment is ground to biology before enzyme digestion reaction, to ensure that the later stage more fully digests, enzymolysis efficiency, structure is improved
Simply, it is convenient to operation, with preferable application prospect.
Description of the drawings
The content and the labelling in figure expressed by the accompanying drawing of this specification is briefly described below：
Fig. 1 is this utility model bionic enzymatic device structure schematic diagram；
Fig. 2 is breaker structural representation；
Fig. 3 is first order enzymolysis device structural representation；
Fig. 4 is second level enzymolysis device structural representation；
Fig. 5 purifying plant structural representations.
It is labeled as in figure：1st, breaker；11st, disintegrating machine shell；12nd, disintegrating machine inner shell；13rd, feed(raw material)inlet；14th, crush
Mechanism；141st, motor is crushed, 142, disintegrating machine rotating shaft；143rd, screw feeder blade is crushed；144th, reclaiming plate；
2nd, first order enzymolysis device；21st, first order enzymatic vessel, 22, rabbling mechanism；221st, stirring motor；222nd, shaft；
223rd, stirring vane；23rd, filter cavity；24th, return duct；25th, kuppe；26th, ultrafilter membrane I；27th, one-level zymolyte discharging opening；
3rd, second level enzymolysis device；31st, second level enzymatic vessel, 32, enzyme digestion reaction pipeline；33rd, vibrator；34th, one-level enzyme
Solution thing charging aperture；35th, trypsin conveying mouth；36th, two grades of zymolyte discharging openings；
4th, trypsin storage tank；5th, pepsin storage tank；6th, hydrochloric acid storage tank；7th, ptyalin storage tank；
8th, pusher；81st, motor is pushed；82nd, rotating shaft is pushed；83rd, blade is pushed；84th, discharge gate；
9th, purifying plant；91st, outer tank is purified, 92, tophan box；93rd, ultrafilter membrane II；94th, it is tank bottoms；95th, discharging opening；96th, two
Level zymolyte charging aperture；97th, tophan box charging aperture.
Specific embodiment
Below against accompanying drawing, by the description to embodiment, specific embodiment of the present utility model is made further in detail
Thin explanation.
This biological enzymolysis pretreatment unit of this utility model, including disintegrating machine shell 11, disintegrating machine inner shell 12 and connection
The crushing mechanism 14 of crushing grinding raw material is provided with the feed(raw material)inlet 13 of disintegrating machine inner shell 12, disintegrating machine inner shell 12.
As shown in figure 1, bionic enzymatic device includes the breaker 1 being sequentially connected, first order enzymolysis device 2 and the second level
Enzymolysis device 3, also includes controlling the controller of the enzymolysis equipment operation work.Each device is controlled by terminal controller
Work start and stop opportunity, and linking discharge charging opportunity, to realize Intelligent control.In this utility model, first order enzymolysis device 2 connects
Pepsin storage tank 5 is connected to, the material Jing after the grinding of breaker 1 is broken is delivered in first order enzymolysis device 2, opens stomach
The material inlet valve of protease storage tank 5, into the first order enzymolysis device 2 a certain amount of pepsin is added, to realize the
Pepsin enzyme digestion reaction is carried out in one-level enzymolysis device 2；The reacted product of Jing first order enzymolysis device 2 is input into second level enzyme
Solution device 3, the connection trypsin of second level enzymolysis device 3 storage tank 4, opens the material inlet valve of trypsin storage tank 4, to this
A certain amount of trypsin is added in second level enzymolysis device 3, to realize carrying out trypsin enzyme in second level enzymolysis device 3
Solution reaction.Small active peptides are broken down in the protein after two-stage enzyme digestion reaction, small-molecular peptides have can directly be disappeared
Change the features such as road absorbs, transport speed is fast, it is low to consume energy and is difficult saturation, produce with certain from biological enzymolysis in vivo as needed
The medicine of effect, generates small-molecular peptides also less to bioavailability height, active strong, side reaction using this equipment enzymolysis.
In this utility model, it is mainly used for material crushing grinding into shape is wasted in breaker 1, in order to follow-up enzyme
The carrying out of solution preocess.The position that pepsin is mainly digested is the peptide bond of the composition such as Phenylalanine, L-Tyrosine and leucine, pancreas egg
The position that white enzyme is mainly digested is the peptide bond of the basic amino acids such as arginine, lysine composition, targetedly selects protease
Preferable small peptide can be obtained, corresponding peptide bond in albumen is digested respectively, active small peptide is obtained.
In this utility model, as shown in Figure 2, breaker 1 includes disintegrating machine shell 11 and disintegrating machine inner shell 12 and connects
The crushing mechanism 14 of crushing grinding raw material is provided with the feed(raw material)inlet 13 of logical disintegrating machine inner shell 12, disintegrating machine inner shell 12.Disintegrating machine
Structure 14 includes broken motor 141, crushes the outfan connection disintegrating machine rotating shaft 142 of motor 141, and disintegrating machine rotating shaft 142 is provided with
Broken screw feeder blade 143.During work, there is feed(raw material)inlet 13 to add to disintegrating machine inner shell 12 raw material, started by controller
Broken motor 141 drives disintegrating machine rotating shaft 142 and broken screw feeder blade 143 thereon that broken grinding is carried out to the material in inner shell
Mill.
Disintegrating machine rotating shaft 142 is extended straight down by the upper end of disintegrating machine inner shell 12, and the lower end of disintegrating machine rotating shaft 142 is provided with
Reclaiming plate 144, reclaiming plate 144 be provided with it is some go out material through hole.Shape is wasted Jing after the grinding of crushing mechanism 14 is crushed to a certain degree
The material through hole that goes out of thing Jing squeezing actions Jing reclaiming plate 144 is extruded to the disintegrating machine shell 11 of lower section.
In this utility model, the bottom of disintegrating machine shell 11 of breaker 1 is provided with and outwards exports fragment to next
The pusher 8 of reflection device.The nearly pusher 8 of fragment is pushed to first order enzyme by breaker 1 in this utility model
In solution device 2, one-level enzyme digestion reaction is carried out.
In this utility model, it is preferred that pusher 8 includes the push rotating shaft 82 of horizontal cross arrangement, turn located at pushing
The push motor 81 for pushing blade 83 and the drive push rotation work of rotating shaft 82 on axle 82.Controller control pushes motor 81 and opens
Dynamic control push rotating shaft 82 is rotated to drive and push thereon the agitator type of blade 83 to push ahead.Push rotating shaft 82 and stretch out disintegrating machine shell
11 one end is provided with discharge gate 84, and discharge gate 84 is connected with the charging aperture of first order enzymolysis device 2.Broken product Jing is pushed
Blade 83 is acted on, the orderly push forward of rule, and Jing discharge gates 84 are entered in first order enzymolysis device 2.Preferably, discharge gate 84
Be along pushing the pyramidal structure that tapers into of direction section, in order to fragment it is orderly be sent to first order enzymolysis device 2
It is interior, it is to avoid solid accumulation clogging occur.
In this utility model, it is preferred that be additionally provided with ptyalin storage tank 7, ptyalin in the outside of breaker 1
It is delivered in disintegrating machine inner shell 12 via ptyalin delivery conduit.Ptyalin delivery conduit is the vertical high pressure for extending
Delivery pipe, on the wall of ptyalin delivery conduit side some spray orifices are provided with.In actual mechanical process, can as needed to broken
The ptyalin that addition is extracted in machine inner shell 12, with auxiliary catalysis starch water maltose is decomposed into.
In this utility model, as shown in Figure 3, first order enzymolysis device 2 includes first order enzymatic vessel 21, and located at first
Rabbling mechanism 22 in level enzymatic vessel 21.Rabbling mechanism 22 includes shaft 222 and the stirring vane located at the lower end of shaft 222
223, shaft 222 drives rotation work by stirring motor 221, and stirring motor is by controller controlled motor start and stop.
Hydrochloric acid storage tank 6 is additionally provided with first order enzymatic vessel 21, built with to adjust pH value in first order enzymatic vessel 21
Dilute hydrochloric acid.First order enzymolysis device 2 is additionally provided with the first attemperating unit for controlling its tank body interior reaction temperature.Attemperating unit
Including heater, thermometer and the first temperature sensor of controller is connected to.Heated up by heating devices heat, temperature is passed
Temperature Feedback in first order enzymatic vessel 21 to controller is controlled its temperature value in setting by sensor by controller, to protect
The optimal reaction environment of card.
The course of work of this first order enzymolysis device 2 is, the injection water and certain density in the first order enzymatic vessel 21
Dilute hydrochloric acid, adjustment reaction liquid environment pH value is 1.0 to 5.0, to ensure to activate pepsin, there is provided the suitable environment for decomposing.
A certain amount of pepsin is added, is started rabbling mechanism 22 and is stirred evenly oar, start-up temperature controller is heated to 28 DEG C～40 DEG C guarantors
Temperature enzymolysis 2～6 hours, carries out enzyme digestion reaction, and stir synchronously is carried out in the process, to obtain sufficient hydrolysis result.
In this utility model, the lower section of first order enzymatic vessel 21 is provided with the filter cavity 23 filtered to reactants separate, filters
Ultrafilter membrane I 26 is provided with cavity 23.The lower section of first order enzymatic vessel 21 is provided with the horn-like kuppe of the opening of ultrafilter membrane I 26
25, material after reaction can be completely sent on the ultrafilter membrane I 26 of lower section by the way that kuppe 25 is wider array of, it is most fast most wide to realize
General filtration.Filter cavity 23 is located at the cavity of the top of ultrafilter membrane I 26 and is communicated to first order enzymatic vessel 21 by return duct 24；
The one-level zymolyte discharging opening 27 for being connected to second level enzymolysis device 3 is provided with positioned at the side wall of the lower section cavity of ultrafilter membrane I 26.Jing
After first order enzymolysis, the product after one-level is digested completely can be filtered to lower section by ultrafilter membrane I 26, can be by positioned at ultrafilter membrane I
One-level zymolyte discharging opening 27 on 26 lower section cavity walls, delivers to second level enzymolysis device 3, carries out next stage enzyme digestion reaction；
And the product of unreacted complete larger molecular weight then can not be stayed in cavity above it, top by ultrafilter membrane I 26 by filtration
Cavity in material first order enzymatic vessel 21 is communicated to by return duct 24, the reflux course can be flowed back by high pressure delivery,
The pressure differential in the cavity and first order enzymatic vessel 21 is controlled by controller and realizes backflow, the equipment can setting can improve life
The decomposition efficiency of thing, it is ensured that the purity and activity of final extract.
In this utility model, as shown in Figure 4, second level enzymolysis device 3 includes second level enzymatic vessel 31 and located at second
Enzyme digestion reaction pipeline 32 in level enzymatic vessel 31, enzyme digestion reaction pipeline 32 is provided with one-level zymolyte charging aperture 34 and connection pancreas egg
The trypsin conveying mouth 35 of white enzyme storage tank 4.Using the second level enzymolysis device 3 to Jing after first order enzymolysis device 2 decomposes
Product further digest, to obtain small active peptides.The memory storage of trypsin storage tank 4 is with tryptic anti-
Liquid is answered, its aqueous ph value can be adjusted to 8.0 to 10, to ensure tryptic activity using sodium hydroxide, there is provided suitable to decompose
Environment.
Preferably, as shown in Figure 4, enzyme digestion reaction pipeline 32 is the serpentine-like bending being arranged in second level enzymatic vessel 31
Pipeline, can extend reactant and catalytic materials tryptic response time, increase its exposure level, improve reaction efficiency.And should
The wall of enzyme digestion reaction pipeline 32 is made using ultrafilter membrane, and herein the aperture of ultrafilter membrane selects to ensure to react the small-molecular peptides for generating
Can filter, the material of other larger molecular weights not may pass through.Preferably vibrator 33 is provided with the both sides of second level enzymatic vessel 31,
Can ensure that product timely discharges enzyme digestion reaction pipeline 32, to improve reaction efficiency.
The waste gate of waste material after the lower end of enzyme digestion reaction pipeline 32 is provided with and discharges reaction.The lower end of second level enzymatic vessel 31
It is provided with two grades of zymolyte discharging openings 36.
In this utility model, second level enzymolysis device 3 is additionally provided with the second temperature control for controlling its tank body interior reaction temperature
Device.Attemperating unit includes heater, thermometer and is connected to the second temperature sensor of controller.In course of reaction, open
Dynamic temperature controller, keeps tank interior reaction temperature to be heated to 40 DEG C～50 DEG C insulations and digests 4～5 hours, carries out enzyme digestion reaction,
The synchronous vibration of vibrator 33 during this, to obtain sufficient hydrolysis result.
In this utility model, it is preferred that the biological enzymolysis pretreatment unit is additionally provided with detection control course of reaction respectively
The PH detectors of pH value in first order enzymolysis device 2 and second level enzymolysis device 3, by the way that real-time detection signal is transferred to into control
Device processed, when its pH value deviates the range of normal value of setting, controller controls to be digested to first order enzymatic vessel 21 or the second level
The corresponding dilute hydrochloric acid solution of addition or sodium hydroxide solution are supplemented in tank 31 to adjust pH value setting value, it is ensured that normal effective
Enzyme digestion reaction, improve reaction efficiency.
This utility model bionic enzymatic equipment is additionally provided with to be placed in after second level enzymolysis device 3 and produces to purify two grades of enzymolysis
The purifying plant 9 of thing.Purifying plant 9 includes tophan box 92, the purification outer tank 91 being placed on the outside of tophan box, and tophan box 92 is by rotating
Motor drives high-speed rotation, and on the side wall of tophan box 92 some ultrafiltration holes are provided with.Tophan box 92 is provided with tophan box charging aperture 97,
Purification outer tank 91 is provided with two grades of zymolyte charging apertures 96 of the tophan box charging aperture 97 that aligns when tophan box 92 is static.
Product Jing delivery pipes connection after enzymolysis is sent to two grades of zymolyte discharging openings 36 two grades of enzymolysis of purifying plant 9
Thing charging aperture 96, two grades of zymolyte charging apertures 96 align with the tophan box charging aperture 97 on tophan box under resting state 92, with
Ensure stable and accurate conveying process.Preferably, tophan box charging aperture 97 is the larger funnel-shaped structure of opening.When conveying process
After the completion of by controller control start rotary electric machine drive the high-speed rotation of tophan box 92 be centrifuged operation, in centrifugal force
In the presence of, the ultrafiltration hole of small-molecular peptides Jing tophan boxes 92 is isolated and is collected in lower end purification outer tank 91.Lean in purification outer tank 91
Nearly bottom is provided with ultrafilter membrane II 93, and the small-molecular peptides to isolating carry out final filtration separation, the activity higher to obtain purity
Small-molecular peptides.Preferably, purify outer tank 91 tank bottoms 94 are provided with discharging opening 95, and tank bottoms 94 are in tilted layout towards discharging opening 95,
In order to the output of product.
Small-molecular peptides refer mainly to the peptide containing 2～15 amino acid residues.Small molecule of the relative molecular mass below 3000
Peptide, with directly being absorbed by digestive tract, the features such as transport speed is fast, it is low to consume energy and is difficult saturation, therefore, select suitable super
Filter sizes, in the presence of multiple ultrafilter membrane, final purification screening retains the average molecular matter that can be directly absorbed by gastrointestinal tract
The part of amount ＜ 3000.
This utility model biological enzymolysis pretreatment unit, can be ground to biology stirring pretreatment before enzyme digestion reaction,
To ensure that the later stage more fully digests, enzymolysis efficiency is improved, simple structure is convenient to operation, with preferable application prospect.
This utility model is exemplarily described above in conjunction with accompanying drawing, but this utility model be not limited to it is above-mentioned
Mode, as long as the improvement of the various unsubstantialities carried out using method of the present utility model design and technical scheme or directly application
In other occasions, all fall within protection domain of the present utility model.
Claims (6)
1. a kind of biological enzymolysis pretreatment unit, it is characterised in that：Including disintegrating machine shell (11), disintegrating machine inner shell (12) and company
Lead in the feed(raw material)inlet (13) of the disintegrating machine inner shell (12), the disintegrating machine inner shell (12) and be provided with the broken of crushing grinding raw material
Mechanism (14)；The crushing mechanism (14) is including broken motor (141), and the outfan connection of the broken motor (141) is broken
Machine rotating shaft (142), the disintegrating machine rotating shaft (142) is provided with broken screw feeder blade (143)；Outside the disintegrating machine of breaker (1)
Shell (11) bottom is provided with the pusher (8) outwards exported fragment to next reaction unit.
2. according to the biological enzymolysis pretreatment unit described in claim 1, it is characterised in that：The disintegrating machine rotating shaft (142) by
The upper end of the disintegrating machine inner shell (12) extends straight down, and the lower end of the disintegrating machine rotating shaft (142) is provided with reclaiming plate
(144), the reclaiming plate (144) be provided with it is some go out material through hole.
3. according to the biological enzymolysis pretreatment unit described in claim 1, it is characterised in that：Breaker (1) outside is provided with saliva
Liquid amylase storage tank (7), ptyalin is delivered in the disintegrating machine inner shell (12) via ptyalin delivery conduit.
4. according to the biological enzymolysis pretreatment unit described in claim 3, it is characterised in that：The ptyalin delivery conduit
For the vertical high-pressure delivery pipe for extending, on the ptyalin delivery conduit side wall some spray orifices are provided with.
5. according to the biological enzymolysis pretreatment unit described in claim 1, it is characterised in that：The pusher (8) is including water
The push rotating shaft (82) of flat lateral arrangement, the described push of push blade (83) and drive on push rotating shaft (82) turns
The push motor (81) of axle (82) rotation work.
6. according to the biological enzymolysis pretreatment unit described in claim 5, it is characterised in that：Push rotating shaft (82) stretches out institute
The one end for stating disintegrating machine shell (11) is provided with the charging of discharge gate (84), the discharge gate (84) and first order enzymolysis device (2)
Mouth connection, the discharge gate (84) is the pyramidal structure tapered into along push direction section.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620752792.2U CN206109412U (en) | 2016-07-15 | 2016-07-15 | Biological enzymolysis preprocessing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620752792.2U CN206109412U (en) | 2016-07-15 | 2016-07-15 | Biological enzymolysis preprocessing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206109412U true CN206109412U (en) | 2017-04-19 |
Family
ID=58509259
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620752792.2U Expired - Fee Related CN206109412U (en) | 2016-07-15 | 2016-07-15 | Biological enzymolysis preprocessing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206109412U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107034139A (en) * | 2017-04-26 | 2017-08-11 | 黑龙江省野生动物研究所 | A kind of deer whip active peptide extraction element and extracting method |
| CN108795094A (en) * | 2017-11-02 | 2018-11-13 | 安发（福建）生物科技有限公司 | A kind of pitch-dark pigment extraction control device of marine animal |
-
2016
- 2016-07-15 CN CN201620752792.2U patent/CN206109412U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107034139A (en) * | 2017-04-26 | 2017-08-11 | 黑龙江省野生动物研究所 | A kind of deer whip active peptide extraction element and extracting method |
| CN108795094A (en) * | 2017-11-02 | 2018-11-13 | 安发（福建）生物科技有限公司 | A kind of pitch-dark pigment extraction control device of marine animal |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170419 Termination date: 20210715 |
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| CF01 | Termination of patent right due to non-payment of annual fee |