CN205803494U - A kind of trypsin digestion device - Google Patents

Abstract

The utility model discloses a kind of trypsin digestion device, including second level enzymatic vessel (31) and the enzyme digestion reaction pipeline (32) be located in described second level enzymatic vessel (31), described enzyme digestion reaction pipeline (32) is provided with one-level zymolyte charging aperture (34) and the trypsin conveying mouth (35) of connection trypsin storage tank (4).That this utility model one trypsin digestion device operates with is convenient, efficiency is high, safety good, has preferable application prospect.

Description

A kind of trypsin digestion device

Technical field

This utility model belongs to zymolysis technique field, more particularly, it relates to a kind of trypsin digestion device.

Background technology

Traditional material extracting mode mostly uses and stays powder, water to carry or the mode such as low-temperature homogenate, this kind of mode or life Thing availability is low, or side reaction is many.Current industrial main employing acid-hydrolysis method produces HVP.But acid hydrolytic reaction condition swashs Strong, aminoacid can be destroyed, additionally, acid-hydrolysis method can produce carcinogen；Also there is employing enzyme hydrolysis method, but enzymolysis equipment is single, enzyme Solve inefficiency, it is impossible to meet the needs of people.

Utility model content

The purpose of this utility model is to solve the problem that prior art exists, it is provided that one operates with is convenient, efficiency is high, The trypsin digestion device that safety is high.

To achieve these goals, the technical scheme that this utility model is taked is this trypsin digestion that provided Device, including second level enzymatic vessel and be located at the enzyme digestion reaction pipeline in the enzymatic vessel of the described second level, described enzyme digestion reaction pipeline It is provided with one-level zymolyte charging aperture and connects the trypsin conveying mouth of described trypsin storage tank.

For making technique scheme more detailed and concrete, this utility model also provides for optimization technique further below Scheme, to obtain satisfied practical function:

Described enzyme digestion reaction pipeline is serpentine-like to be arranged in described second level enzymatic vessel crooked pipeline, described enzyme digestion reaction pipe Road wall is made up of ultrafilter membrane.

Described enzyme digestion reaction lower end of duct is provided with the waste gate of waste material after discharge is reacted.

Enzymatic vessel lower end, the described second level is provided with two grades of zymolyte discharging openings.

Described trypsin digestion device is additionally provided with to control the second attemperating unit of its tank body interior reaction temperature.

Described attemperating unit includes heater, thermometer and be connected to the second temperature sensor of described controller.

This utility model compared with prior art, has the advantage that this utility model one trypsin digestion device, Its intelligence degree is high, uses the method for bionic enzymatic to make small-molecular peptides, controls every grade as far as possible instead in enzymolysis process Should carry out enzymolysis under optimum enzymatic hydrolysis condition, to ensure enzymolysis more fully, improve enzymolysis efficiency, safety is high, and this enzyme The suitability of solution equipment is strong, has preferable application prospect.

Accompanying drawing explanation

Below the content expressed by the accompanying drawing of this specification and the labelling in figure are briefly described:

Fig. 1 is this utility model bionic enzymatic device structure schematic diagram；

Fig. 2 is breaker structural representation；

Fig. 3 is first order enzymolysis device structural representation；

Fig. 4 is trypsin digestion apparatus structure schematic diagram；

Fig. 5 purifying plant structural representation.

Figure is labeled as: 1, breaker；11, disintegrating machine shell；12, disintegrating machine inner shell；13, feed(raw material)inlet；14, broken Mechanism；141, broken motor, 142, disintegrating machine rotating shaft；143, broken screw feeder blade；144, reclaiming plate；

2, first order enzymolysis device；21, first order enzymatic vessel, 22, rabbling mechanism；221, stirring motor；222, shaft； 223, stirring vane；23, filter cavity；24, return duct；25, kuppe；26, ultrafilter membrane I；27, one-level zymolyte discharging opening；

3, trypsin digestion device；31, second level enzymatic vessel, 32, enzyme digestion reaction pipeline；33, vibrator；34, one-level Zymolyte charging aperture；35, trypsin conveying mouth；36, two grades of zymolyte discharging openings；

4, trypsin storage tank；5, pepsin storage tank；6, hydrochloric acid storage tank；7, ptyalin storage tank；

8, pusher；81, motor is pushed；82, rotating shaft is pushed；83, blade is pushed；84, discharge gate；

9, purifying plant；91, outer tank is purified, 92, tophan box；93, ultrafilter membrane II；94, tank bottoms；95, discharging opening；96, two Level zymolyte charging aperture；97, tophan box charging aperture.

Detailed description of the invention

Below against accompanying drawing, by the description to embodiment, detailed description of the invention of the present utility model is made the most in detail Thin explanation.

This utility model this trypsin digestion device, as shown in Figure 4, including second level enzymatic vessel 31 and be located at described Enzyme digestion reaction pipeline 32 in second level enzymatic vessel 31, enzyme digestion reaction pipeline 32 is provided with one-level zymolyte charging aperture 34 and connection The trypsin conveying mouth 35 of trypsin storage tank 4.

As it is shown in figure 1, include the breaker 1 being sequentially connected with, first order enzymolysis device 2 and trypsin digestion device 3, Also include the controller controlling this enzymolysis equipment operation work.When controlling the work start and stop of each device by terminal controller Machine, and linking discharge charging opportunity, to realize Intelligent control.In this utility model, first order enzymolysis device 2 connects pepsin Enzyme storage tank 5, the material ground after crushing through breaker 1 is delivered in first order enzymolysis device 2, opens pepsin storage The material inlet valve of tank 5, adds a certain amount of pepsin in this first order enzymolysis device 2, to realize filling at first order enzymolysis Pepsin enzyme digestion reaction is carried out in putting 2；Input to trypsin digestion device 3 through the reacted product of first order enzymolysis device 2, Trypsin digestion device 3 connects trypsin storage tank 4, opens the material inlet valve of trypsin storage tank 4, to this Trypsin A certain amount of trypsin is added, to realize carrying out trypsin digestion in trypsin digestion device 3 in enzyme enzymolysis device 3 Reaction.Being broken down into small active peptides at protein after two-stage enzyme digestion reaction, small-molecular peptides has can be the most digested Road absorbs, transport speed is fast, consume energy low and be difficult to the features such as saturated, and in organism, enzymolysis is produced and had certain merit as required The medicine of effect, small-molecular peptides is high to bioavailability, activity is strong, side reaction is the least to use this equipment enzymolysis to generate.

In this utility model, it is mainly used for becoming to waste shape by material crushing grinding in breaker 1, in order to follow-up enzyme The carrying out of solution preocess.The position of the main enzymolysis of pepsin is the peptide bond of the compositions such as phenylalanine, tyrosine and leucine, pancreas egg The peptide bond that position is the basic amino acid such as arginine, lysine composition of the white main enzymolysis of enzyme, selects protease targetedly Preferable little peptide can be obtained, peptide bond corresponding in albumen is carried out enzymolysis respectively, available active small peptide.

In this utility model, as shown in Figure 2, breaker 1 includes disintegrating machine shell 11 and disintegrating machine inner shell 12 and connects The feed(raw material)inlet 13 of logical disintegrating machine inner shell 12, is provided with the crushing mechanism 14 of crushing grinding raw material in disintegrating machine inner shell 12.Disintegrating machine Structure 14 includes that broken motor 141, the outfan of broken motor 141 connect disintegrating machine rotating shaft 142, and disintegrating machine rotating shaft 142 is provided with Broken screw feeder blade 143.During work, raw material has feed(raw material)inlet 13 add to disintegrating machine inner shell 12, is started by controller Broken motor 141 drives disintegrating machine rotating shaft 142 and broken screw feeder blade 143 thereon that material in inner shell carries out broken grinding Mill.

Disintegrating machine rotating shaft 142 is extended straight down by the upper end of disintegrating machine inner shell 12, and the lower end of disintegrating machine rotating shaft 142 is provided with Reclaiming plate 144, reclaiming plate 144 be provided with some go out material through hole.Through crushing mechanism 14 grind be crushed to a certain degree after waste shape Thing is extruded to the disintegrating machine shell 11 of lower section through the material through hole that goes out of reclaiming plate 144 through squeezing action.

In this utility model, it is provided with bottom the disintegrating machine shell 11 of breaker 1 and outwards exports crushing thing to next The pusher 8 of reflection device.In this utility model, breaker 1 pushes to first order enzyme by crushing the nearly pusher of thing 8 Solve in device 2, carry out one-level enzyme digestion reaction.

In this utility model, it is preferred that pusher 8 includes the propelling movement rotating shaft 82 that horizontal cross is arranged, it is located at propelling movement and turns Pushing blade 83 and driving the propelling movement motor 81 pushing rotating shaft 82 rotation work on axle 82.Controller controls to push motor 81 and opens Moving control propelling movement rotating shaft 82 rotation drives propelling movement blade 83 agitator type on it to push ahead.Push rotating shaft 82 and stretch out disintegrating machine shell One end of 11 is provided with discharge gate 84, and discharge gate 84 connects with the charging aperture of first order enzymolysis device 2.Broken product is through pushing Blade 83 acts on, and the propelling movement forward that rule is orderly, in discharge gate 84 enters first order enzymolysis device 2.Preferably, discharge gate 84 For along pushing the pyramidal structure that tapers into of cross section, direction, in order to crushed thing orderly be sent to first order enzymolysis device 2 In, it is to avoid solid accumulation clogging occurs.

In this utility model, it is preferred that be additionally provided with ptyalin storage tank 7, ptyalin outside breaker 1 It is delivered in disintegrating machine inner shell 12 via ptyalin delivery conduit.Ptyalin delivery conduit is the high pressure vertically extended Conveying pipe, is provided with some spray orifices on ptyalin delivery conduit sidewall.In actual mechanical process, can be as required to broken The ptyalin extracted is added, with auxiliary catalysis starch water decomposition as maltose in machine inner shell 12.

In this utility model, as shown in Figure 3, first order enzymolysis device 2 includes first order enzymatic vessel 21, and is located at first Rabbling mechanism 22 in level enzymatic vessel 21.Rabbling mechanism 22 includes shaft 222 and is located at the stirring vane of shaft 222 lower end 223, shaft 222 is driven rotation work by stirring motor 221, and stirring motor is controlled motor start and stop by controller.

Hydrochloric acid storage tank 6 it is additionally provided with, built with in order to regulate pH value in first order enzymatic vessel 21 on first order enzymatic vessel 21 Dilute hydrochloric acid.First order enzymolysis device 2 is additionally provided with to control the first attemperating unit of its tank body interior reaction temperature.Attemperating unit Including heater, thermometer and be connected to the first temperature sensor of controller.Being heated up by heating devices heat, temperature passes Sensor, by the Temperature Feedback in first order enzymatic vessel 21 to controller, controls its temperature value in setting by controller, to protect Demonstrate,prove optimal reaction environment.

The work process of this first order enzymolysis device 2 is, injects water and certain density in this first order enzymatic vessel 21 Dilute hydrochloric acid, adjusting reaction liquid environment pH value is 1.0 to 5.0, to ensure to activate pepsin, it is provided that the suitable environment decomposed. Adding a certain amount of pepsin, start rabbling mechanism 22 and stir even oar, start-up temperature controller is heated to 28 DEG C～40 DEG C guarantors Temperature enzymolysis 2～6 hours, carries out enzyme digestion reaction, and stirring synchronization is carried out, to obtain sufficient hydrolysis result in the process.

In this utility model, below first order enzymatic vessel 21, it is provided with the filter cavity 23 that reactants separate is filtered, filters Ultrafilter membrane I 26 it is provided with in cavity 23.The horn-like kuppe of ultrafilter membrane I 26 opening it is provided with below first order enzymatic vessel 21 25, can be the widest to realize by material after reaction by the wider array of ultrafilter membrane I 26 being sent to lower section completely of kuppe 25 General filtration.The cavity that filter cavity 23 is positioned at above ultrafilter membrane I 26 is communicated to first order enzymatic vessel 21 by return duct 24；? It is positioned at the sidewall of cavity below ultrafilter membrane I 26 and is provided with the one-level zymolyte discharging opening 27 being connected to trypsin digestion device 3. After first order enzymolysis, the product after the complete enzymolysis of one-level can be filtered to lower section by ultrafilter membrane I 26, can be by being positioned at ultrafilter membrane Below I 26, the one-level zymolyte discharging opening 27 on cavity wall, delivers to trypsin digestion device 3, carries out next stage enzymolysis anti- Should；The product of the complete larger molecular weight of unreacted then can not pass through ultrafilter membrane I 26, is stayed above it in cavity by filtration, Material in the cavity of top is communicated to first order enzymatic vessel 21 by return duct 24, and this reflux course can be returned by high pressure delivery Stream, controls the pressure differential in this cavity and first order enzymatic vessel 21 by controller and realizes backflow, this equipment can setting can carry High biological decomposition efficiency, it is ensured that the purity of final extract and activity.

In this utility model, as shown in Figure 4, trypsin digestion device 3 includes second level enzymatic vessel 31 and is located at Enzyme digestion reaction pipeline 32 in two grades of enzymatic vessels 31, enzyme digestion reaction pipeline 32 is provided with one-level zymolyte charging aperture 34 and connection pancreas The trypsin conveying mouth 35 of protease storage tank 4.Use this trypsin digestion device 3 to through first order enzymolysis device 2 points The further enzymolysis of product after solution, to obtain small active peptides.Storage for having trypsin in trypsin storage tank 4 Reactant liquor, its aqueous ph value can use sodium hydroxide to regulate to 8.0 to 10, to ensure tryptic activity, it is provided that suitable The environment decomposed.

Preferably, as shown in Figure 4, enzyme digestion reaction pipeline 32 is the serpentine-like bending being arranged in second level enzymatic vessel 31 Pipeline, can extend reactant and catalytic materials tryptic response time, increases its exposure level, improves reaction efficiency.And should Enzyme digestion reaction pipeline 32 wall uses ultrafilter membrane to make, and the aperture of ultrafilter membrane selects to ensure the small-molecular peptides that reaction generates herein Can filter, the material of other larger molecular weights not may pass through.Preferably it is provided with vibrator 33 in the both sides of second level enzymatic vessel 31, Can ensure that product discharges enzyme digestion reaction pipeline 32 timely, to improve reaction efficiency.

The waste gate of waste material after enzyme digestion reaction pipeline 32 lower end is provided with and discharges reaction.Second level enzymatic vessel 31 lower end It is provided with two grades of zymolyte discharging openings 36.

In this utility model, trypsin digestion device 3 is additionally provided with to control the second temperature of its tank body interior reaction temperature Control device.Attemperating unit includes heater, thermometer and be connected to the second temperature sensor of controller.In course of reaction, Start-up temperature controller, keeps tank interior reaction temperature to be heated to 40 DEG C～50 DEG C insulation enzymolysis 4～5 hours, carries out enzyme digestion reaction, Vibrator 33 synchronous vibration in the process, to obtain sufficient hydrolysis result.

In this utility model, it is preferred that this one trypsin digestion device is additionally provided with detection respectively and controls course of reaction The PH detector of pH value in middle first order enzymolysis device 2 and trypsin digestion device 3, by transmitting real-time detection signal To controller, when the range of normal value that the deviation of its pH value sets, controller controls to first order enzymatic vessel 21 or the second level Supplement the corresponding dilute hydrochloric acid solution of interpolation in enzymatic vessel 31 or sodium hydroxide solution is to adjust pH value setting value, it is ensured that be normal Effective enzyme digestion reaction, improves reaction efficiency.

This trypsin digestion device of this utility model is additionally provided with after being placed in trypsin digestion device 3 in order to purify two grades The purifying plant 9 of enzymatic hydrolysate.Purifying plant 9 includes tophan box 92, is placed in the purification outer tank 91 outside tophan box, tophan box 92 Driven high-speed rotation by rotary electric machine, tophan box 92 sidewall is provided with some ultrafiltration holes.Tophan box 92 is provided with tophan box and enters Material mouth 97, purifies outer tank 91 and is provided with when tophan box 92 is static two grades of zymolyte charging apertures of the tophan box charging aperture 97 that aligns 96。

Product after enzymolysis is connected the two grades of enzymolysis being sent to purifying plant 9 by two grades of zymolyte discharging openings 36 through conveying pipe Thing charging aperture 96, two grades of zymolyte charging apertures 96 align with the tophan box charging aperture 97 on tophan box under resting state 92, with Ensure stable and accurate conveying process.Preferably, tophan box charging aperture 97 is the funnel-shaped structure that opening is bigger.When conveying process Controlling to start rotary electric machine by controller after completing drives tophan box 92 high-speed rotation to be centrifuged detached job, at centrifugal force Effect under, small-molecular peptides tank 92 ultrafiltration hole by centrifugation is isolated and is collected to lower end and purifies in outer tank 91.Lean on purifying outer tank 91 Nearly bottom is provided with ultrafilter membrane II 93, isolated small-molecular peptides is carried out final filtration separation, the activity higher to obtain purity Small-molecular peptides.Preferably, purify outer tank 91 tank bottoms 94 are provided with discharging opening 95, and tank bottoms 94 are in tilted layout towards discharging opening 95, So that the output of product.

Small-molecular peptides refers mainly to the peptide containing 2～15 amino acid residues.The relative molecular mass little molecule below 3000 Peptide, have can direct digested road absorbs, transport speed is fast, consume energy low and be difficult to the features such as saturated, therefore, select super Filter sizes, under the effect of multiple ultrafilter membrane, final purification screens the average molecular matter that reservation directly can be absorbed by gastrointestinal tract The part of amount ＜ 3000.

This utility model trypsin digestion device, its intelligence degree is high, uses the method for bionic enzymatic to make little point Sub-peptide, controls every order reaction in enzymolysis process as far as possible and carries out enzymolysis under optimum enzymatic hydrolysis condition, more abundant to ensure Enzymolysis, improve enzymolysis efficiency, safety is high, and the suitability of this enzymolysis equipment is strong, has preferable application prospect.

Above in conjunction with accompanying drawing, this utility model is exemplarily described, but this utility model is not limited to above-mentioned Mode, as long as using the improvement of the various unsubstantialities that method of the present utility model is conceived and technical scheme is carried out or directly applying In other occasion, all fall within protection domain of the present utility model.

Claims (6)

1. a trypsin digestion device, it is characterised in that: include second level enzymatic vessel (31) and be located at described second level enzyme Solve the enzyme digestion reaction pipeline (32) in tank (31), described enzyme digestion reaction pipeline (32) be provided with one-level zymolyte charging aperture (34) and The trypsin conveying mouth (35) of connection trypsin storage tank (4).

2. according to the trypsin digestion device described in claim 1, it is characterised in that: described enzyme digestion reaction pipeline (32) be in Snakelike described second level enzymatic vessel (31) crooked pipeline that is arranged in, described enzyme digestion reaction pipeline (32) wall is made up of ultrafilter membrane.

3. according to the trypsin digestion device described in claim 2, it is characterised in that: described enzyme digestion reaction pipeline (32) lower end It is provided with the waste gate of waste material after discharge is reacted.

4. according to the trypsin digestion device described in claim 3, it is characterised in that: enzymatic vessel (31) lower end, the described second level It is provided with two grades of zymolyte discharging openings (36).

5. according to the trypsin digestion device described in any one of Claims 1-4, it is characterised in that: described trypsin enzyme Solve device (3) and be additionally provided with to control the second attemperating unit of its tank body interior reaction temperature.

6. according to the trypsin digestion device described in claim 5, it is characterised in that: described attemperating unit includes adding hot charging Put, thermometer and be connected to the second temperature sensor of described controller.