CN111196833A

CN111196833A - Method for extracting momordica saponins from momordica charantia

Info

Publication number: CN111196833A
Application number: CN201811374158.XA
Authority: CN
Inventors: 董妍
Original assignee: Nanjing Siborn Biotechnology Co Ltd
Current assignee: Nanjing Siborn Biotechnology Co Ltd
Priority date: 2018-11-19
Filing date: 2018-11-19
Publication date: 2020-05-26

Abstract

The invention relates to the technical field of medicines, in particular to a method for extracting momordica saponins, which comprises the following processing steps: (1) pulverizing fructus Momordicae Charantiae, and extracting with ethanol under reflux; (2) purifying by macroporous resin; (3) separating by a high-speed counter-current chromatograph; (4) concentrating and drying to obtain fructus Momordicae Charantiae saponin. The invention adopts ethanol reflux extraction, macroporous resin purification and high-speed countercurrent chromatography separation to extract the momordica saponins, has simple process, short extraction time, high extraction rate and low production cost, and can carry out industrialized production.

Description

Method for extracting momordica saponins from momordica charantia

Technical Field

The invention belongs to the field of separation of active ingredients of natural products, and particularly relates to a method for extracting and purifying momordica saponins in momordica charantia.

Background

Momordica charantia L of Cucurbitaceae, which is climbing soft and weak herb, for one year, and has flower and fruit period of 5-10 months. The balsam pear is originally produced in east India and is widely cultivated in the temperate regions of the world. The cultivation is common in south and north China. The balsam pear is sweet and bitter in fruit flavor, is mainly used as vegetables and can also be sugared; the mature pulp and aril can also be eaten. Balsam pear is bitter in taste, cold in nature when it is fresh, warm in nature when it is cooked. Uncooked food can clear summer heat and purge fire, and relieve fever and restlessness; cooked food has effects of nourishing blood, nourishing liver, moistening spleen, invigorating kidney, removing pathogenic heat, relieving fatigue, clearing heart fire, improving eyesight, invigorating qi, and tonifying yang. However, eating Kugua should also pay attention not to damage the qi of spleen and lung. Although the summer weather is hot, people can not eat too much bitter food, and the spicy food (such as hot pepper, shallot and garlic) is preferably matched, so that the bitter taste can be avoided entering the heart, and the lung qi can be benefited.

The extraction of momordica saponins mostly adopts a traditional alkalization extraction method. The traditional extraction method has the defects of large organic solvent usage amount, serious pollution, low extraction rate and the like, and the high-speed counter-current chromatograph is adopted to separate the momordica saponins, so that the plant tissues can be crushed and decomposed mildly, the yield is improved greatly, and the three-dimensional structure of the components is not easy to damage. .

Disclosure of Invention

The invention aims to provide a method for extracting momordica saponins with short extraction time and high yield aiming at the defects of the prior art.

The invention comprises the following steps:

drying and crushing bitter gourd, performing reflux extraction for 1-3 times by using 30-60% ethanol in an amount which is 3-5 times that of the bitter gourd, performing each time for 0.5-1.5 h, filtering, combining extracting solutions, concentrating until no alcohol exists to obtain a concentrated solution, adding the concentrated solution into a macroporous resin column for adsorption and purification, eluting by using 4-8 BV of acid alcohol, collecting eluent, recovering a solvent to dryness, separating the extract of the bitter gourd saponin by using a high-speed counter-current chromatograph, collecting the eluent, concentrating, and drying to obtain the high-purity bitter gourd saponin.

The macroporous resin is AB-8, D-101 and DS-401, and the volume weight ratio of the dosage to the medicinal material is 1: 1-3.

The acid alcohol is a 30-50% ethanol solution containing 1% acetic acid.

The parameters of the high-speed countercurrent chromatograph are set as follows: the rotating speed is 700-1000 r/min, and the flow rate of the mobile phase is 1-5 ml/min.

The present invention will be further described with reference to specific embodiments, but the scope of the invention as claimed is not limited to the following embodiments.

The specific implementation mode is as follows:

example 1:

drying and crushing bitter gourd, performing reflux extraction for 1 time by using 30% ethanol in an amount which is 3 times that of the bitter gourd, performing filtration for 0.5 hour each time, combining extracting solutions, concentrating until no alcohol exists to obtain a concentrated solution, adding the concentrated solution into an AB-8 macroporous resin column for adsorption and purification, wherein the volume-weight ratio of the dosage to the medicinal material amount is 1: eluting with 4BV acid alcohol (the acid alcohol is 30% ethanol solution containing 1% acetic acid), collecting eluate, recovering solvent to dryness, and separating the extract by high speed countercurrent chromatography with the following parameters: rotating at 700r/min, flowing at 1ml/min, collecting eluate, concentrating, and drying to obtain high purity fructus Momordicae Charantiae saponin.

Example 2:

drying and crushing bitter gourd, performing reflux extraction for 3 times by using 60% ethanol in an amount which is 5 times that of the bitter gourd, performing 1.5h each time, filtering, combining extracting solutions, concentrating until no alcohol exists to obtain a concentrated solution, adding the concentrated solution into a D-101 macroporous resin column for adsorption and purification, wherein the volume-weight ratio of the dosage to the medicinal material amount is 1: eluting with 8BV acid alcohol (the acid alcohol is 50% ethanol solution containing 1% acetic acid), collecting eluate, recovering solvent to dryness, and separating the extract by high speed countercurrent chromatography with the following parameters: rotating at 1000r/min and flowing at 5ml/min, collecting eluate, concentrating, and drying to obtain high purity fructus Momordicae Charantiae saponin.

Example 3:

drying and crushing bitter gourd, performing reflux extraction for 3 times by adopting 50% ethanol in an amount which is 4 times that of the bitter gourd, performing 1 hour each time, filtering, combining extracting solutions, concentrating until no alcohol exists to obtain a concentrated solution, adding the concentrated solution into a DS-401 macroporous resin column for adsorption and purification, wherein the volume weight ratio of the using amount to the medicinal material amount is 1: eluting with 6BV acid alcohol (the acid alcohol is 40% ethanol solution containing 1% acetic acid), collecting eluate, recovering solvent to dryness, and separating the extract by high speed countercurrent chromatography with the following parameters: rotating at a speed of 800r/min and a flow rate of 4ml/min, collecting eluate, concentrating, and drying to obtain high-purity momordicoside.

Claims

1. The extraction method of the momordica saponins is characterized by comprising the following steps of:

1) crushing bitter gourds, performing reflux extraction for 1-3 times by using 30-60% ethanol in an amount which is 3-5 times that of the bitter gourds, performing filtration for 0.5-1.5 h each time, combining extracting solutions, and concentrating until no alcohol exists to obtain a concentrated solution;

2) adding the concentrated solution into a macroporous resin column for adsorption and purification, eluting with 4-8 BV of acid alcohol, collecting eluent, and recovering the solvent until the eluent is dry;

3) separating the above Momordica saponins extract with high speed countercurrent chromatography, collecting eluate, concentrating, and drying to obtain high purity Momordica saponins.

2. The extraction method of momordica saponins according to claim 1, wherein the macroporous resin used in step 2) is AB-8, D-101, DS-401, the volume to weight ratio of the amount used to the amount of the medicinal materials is 1: 1-3.

3. A method for extracting momordicoside as claimed in claim 1, wherein the acid alcohol in step 2) is a 30-50% ethanol solution containing 1% acetic acid.

4. A method for extracting momordicoside as claimed in claim 1, wherein the parameters of the high speed countercurrent chromatography in step 3) are set as follows: the rotating speed is 700-1000 r/min, and the flow rate of the mobile phase is 1-5 ml/min.