CN105255946A - Method for extracting fresh fructus momordicae fruit extract by virtue of enzymolysis-ultrasonic wave cooperation - Google Patents
Method for extracting fresh fructus momordicae fruit extract by virtue of enzymolysis-ultrasonic wave cooperation Download PDFInfo
- Publication number
- CN105255946A CN105255946A CN201510752134.3A CN201510752134A CN105255946A CN 105255946 A CN105255946 A CN 105255946A CN 201510752134 A CN201510752134 A CN 201510752134A CN 105255946 A CN105255946 A CN 105255946A
- Authority
- CN
- China
- Prior art keywords
- extract
- enzymolysis
- centrifugal
- grosvenor momordica
- ultrasonic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Fruits And Vegetables (AREA)
Abstract
The invention discloses a method for extracting a fresh fructus momordicae fruit extract by virtue of enzymolysis-ultrasonic wave cooperation. The method comprises the following steps of (1) crushing; (2) extraction; (3) rough filtration; (4) centrifugation; (5) membrane concentration; (6) blending; and (7) spray drying. According to the method, by combining enzymolysis with ultrasonic waves, the process is simplified, the production cost is lowered, the yield of the extract and the content of effective components are improved, and the product with good taste and prominent fragrance is obtained.
Description
Technical field
The present invention relates to technical field of bioengineering, be specifically related to the method that a kind of enzymolysis-ultrasonic synergistic extracts Grosvenor Momordica fresh fruit extract.
Background technology
Grosvenor Momordica is the fruit of the perennial vine of Curcurbitaceae; Another name LAHANGUO, Herba Cardiospermi Halicacabi, Semen Momordicae, invaluable, arhat table, be described as " angle fruit " by people, its leaf is heart-shaped, dioecy, summer-flowering, seed in autumn.Mainly originate in Longjiang township, Yongfu county, Guangxi Zhuang Autonomous Region Guilin City, dragon victory and hundred Shou Deng towns, He Longsheng county, Yongfu county is that township's plantation history of Grosvenor Momordica is longer, wherein plantation Grosvenor Momordica in Yongfu has had more than 300 year history, Long Sheng county plantation Grosvenor Momordica has had more than 200 year history, China's 90 percent Grosvenor Momordica originates in He Longsheng county, Yongfu county, Grosvenor Momordica is the famous and precious local and special products in Guilin, and be also one of medicine-food two-purpose material of the first batch of approval of country, its primary efficacy is can be relieving cough and reducing sputum.Fruits nutrition is worth very high, containing abundant vitamins C (containing 400 milligrams ~ 500 milligrams in every 100 grams of fresh fruits) and glucoside, fructose, glucose, protein, lipid etc.
Fructus Monordicae extract is pale yellow powder.Taste is extremely sweet, and its sugariness is 20-50 times of sweetness of cane sugar, and sweet taste, close to granulated sugar, has the aftertaste being slightly similar to Radix Glycyrrhizae.Sweeter than sucrose 300 times of mogroside contained by it, not producing heat, is the famous and precious raw material of beverage, confectionery industry, is the best substitute of sucrose.Its market application foreground is boundless.
Grosvenor Momordica is as the endemic plant of China, because of the advantage in physical features, for the research of Grosvenor Momordica each side is provided convenience, mainly concentrate on the purifying technique aspect of mogroside V at present, application number be disclose in the patent application of CN101690573A a kind of mogroside V content be greater than 60% Grosvenor Momordica extract upper product method, the method is separated and alumina column chromatography, enzymolysis after Grosvenor Momordica fragmentation, water extraction, concentrated, ion-exchange resin decolorization, macroporous resin adsorption finally by the product of concentrate drying.The method, through more chromatographic enrichment, makes the yield of the finished product lower.Publication number is disclose a kind of method preparing mogroside V from Grosvenor Momordica in the application of CN104086615A, the method be by autoclaving after Grosvenor Momordica fragmentation, supercritical fluid extraction, through resin chromatography, concentrated, dry product, in product, sweet glycosides V purity is more than 70%, but due to supercritical fluid extraction equipment primary treatment quantitative limitation, be unfavorable for continuous print scale production.A kind of extracting and preparing technique (application number: CN200610034872.5) of Fructus Monordicae extract discloses a kind of extract at low temperature preparation technology of Fructus Monordicae extract, a kind of to extract under 40 DEG C and following temperature condition and to utilize Multi-stage precise filters and polyvalent alcohol extraction phase combines method to prepare the nutritive ingredients such as rich vitamin C be main food supplementary material extract and mogroside is simultaneously the preparation technology of the sweeting agent extract led, extract because relating to extract at low temperature and Multi-stage precise filtration and polyvalent alcohol, product yield is lower, solvent dosage is large, technique is also more complicated, cost is higher, be not suitable for suitability for industrialized production.
Summary of the invention
For overcoming the defect of prior art, a kind of enzymolysis-ultrasonic synergistic is the object of the present invention is to provide to extract the method for Grosvenor Momordica fresh fruit extract, be combined with ultrasonic wave by enzymolysis, Simplified flowsheet, reduce production cost, improve the content of extract yield and effective constituent, to obtain the product that mouthfeel is good, delicate fragrance is outstanding.
The technical solution adopted in the present invention is as follows for achieving the above object:
Enzymolysis-ultrasonic synergistic extracts a method for Grosvenor Momordica fresh fruit extract, and the method comprises the following steps:
1) pulverize: select Grosvenor Momordica fresh fruit, to load after adopting pulverizer to pulverize in extractor and to add water-dialyzing;
2) extract: in extractor, add polygalacturonase and cellulase, put into by extractor with ultrasonoscope enzymolysis under the condition of 40-50 DEG C, ultrasonic synergistic extracts 50-90min simultaneously, obtains solidliquid mixture;
3) coarse filtration: by above-mentioned steps 2) in solidliquid mixture double-deck screen shale shaker, removing large granular impurity;
4) centrifugal: through step 3) process after solidliquid mixture dish-style machine centrifugal carry out centrifugal, filter, get liquid;
5) membrane concentration: through step 4) centrifugal after liquid polyamide composite film concentrate;
6) allocate: through step 5) add maltodextrin in enriched material after process and cycloheptaamylose is deployed into mixture;
7) spraying dry: by step 6) mixture carry out spraying dry, control Fructus Monordicae extract moisture content below 5%.
In further scheme, step 2 of the present invention) in the weight of polygalacturonase of adding be the 2-5% of Grosvenor Momordica fresh fruit weight, the weight of the cellulase of interpolation is the 1-3% of Grosvenor Momordica fresh fruit weight.
In further scheme, step 2 of the present invention) in ultrasonic power be 800-1000W, frequency is 20-25KHZ, and ultrasonic intensity is 2-5W/cm
2.
In further scheme, step 3 of the present invention) in, the upper strata mesh screen of double-deck screen shale shaker is 80 orders, and lower floor's mesh screen is 200 orders.
In further scheme, step 4 of the present invention) in centrifugal process, the rotating speed of disk centrifugal separator is 6000-8000 rev/min, inlet amount control bit 3.0-3.5 ton/hour.
In further scheme, concentration time of the present invention controls within 120min, thickening temperature≤40 DEG C.
In further scheme, step 5 of the present invention) in the discharge concentration of concentrated solution be 15.0 ± 1.0Brix.
In further scheme, step 6 of the present invention) in maltodextrin addition accounts for the 20-50% of solid content, cycloheptaamylose accounts for solid content 5-10%.
In further scheme, step 7 of the present invention) spraying dry time, control inlet temperature is 180-210 DEG C; Air outlet temperature is 95-105 DEG C.
Compared to existing technology, beneficial effect of the present invention is:
1. method of the present invention is with new fresh Fructus Momordicae for raw material, and more fresh and sweet than hyperthermia drying Grosvenor Momordica on local flavor, in food, range of application is wider;
2. the present invention adopts polygalacturonase and cellulase to be composited, and hydrolysis result is more desirable; Enzymolysis and ultrasonic extraction act synergistically, and can complete the abundant extraction of new fresh Fructus Momordicae at the temperature of 40-50 DEG C;
3. adopt enzymolysis-ultrasonic synergistic extraction method to prepare Grosvenor Momordica fresh fruit extract in method of the present invention, the extraction yield of fresh fruit extract improves 16% than decocting method formulation, improves 11%, than microwave loss mechanisms 13.5% than ultrasonic extraction;
4. the present invention adopts extract at low temperature farthest to ensure the content of effective constituent in Fructus Monordicae extract, and more fresh and sweet on local flavor, mouthfeel is better;
5. adopt present method to prepare Grosvenor Momordica fresh fruit extract, cost reduces about 15% than ordinary method.
Embodiment
Below in conjunction with concrete embodiment, the present invention is described in further detail.
Enzymolysis-ultrasonic synergistic extracts a method for Grosvenor Momordica fresh fruit extract, and the method comprises the following steps:
1) pulverize: select Grosvenor Momordica fresh fruit, to load after adopting pulverizer to pulverize in extractor and to add water-dialyzing;
2) extract: in extractor, add polygalacturonase and cellulase, put into by extractor with ultrasonoscope enzymolysis under the condition of 40-50 DEG C, ultrasonic synergistic extracts 50-90min simultaneously, obtains solidliquid mixture;
3) coarse filtration: by above-mentioned steps 2) in solidliquid mixture double-deck screen shale shaker, removing large granular impurity;
4) centrifugal: through step 3) process after solidliquid mixture dish-style machine centrifugal carry out centrifugal, filter, get liquid;
5) membrane concentration: through step 4) centrifugal after liquid polyamide composite film concentrate;
6) allocate: through step 5) add maltodextrin in enriched material after process and cycloheptaamylose is deployed into mixture;
7) spraying dry: by step 6) mixture carry out spraying dry, control Fructus Monordicae extract moisture content below 5%.
In further scheme, step 2 of the present invention) in the weight of polygalacturonase of adding be the 2-5% of Grosvenor Momordica fresh fruit weight, the weight of the cellulase of interpolation is the 1-3% of Grosvenor Momordica fresh fruit weight.
In the present invention, enzymolysis and supersound extraction temperature are proportional relations with extracting yield within the specific limits, the selection of temperature needs enzymolysis and supersound extraction efficiency simultaneously, Extracting temperature selected in such scheme farthest can ensure the content of effective constituent in Fructus Monordicae extract, more fresh and sweet on local flavor, mouthfeel is better.Ultrasonic frequency has certain relation on the impact of extracting result with the structure extracting raw material, but is not that power is larger or the higher extraction effect of frequency is better.The present invention reaches the object controlling extract local flavor and yield by control temperature, frequency.In further scheme, step 2 of the present invention) in ultrasonic power be 800-1000W, frequency is 20-25KHZ, and ultrasonic intensity is 2-5W/cm
2.
In further scheme, step 3 of the present invention) in, the upper strata mesh screen of double-deck screen shale shaker is 80 orders, and lower floor's mesh screen is 200 orders.
In further scheme, step 4 of the present invention) in centrifugal process, the rotating speed of disk centrifugal separator is 6000-8000 rev/min, inlet amount control bit 3.0-3.5 ton/hour.
In further scheme, concentration time of the present invention controls within 120min, thickening temperature≤40 DEG C.
In further scheme, step 5 of the present invention) in the discharge concentration of concentrated solution be 15.0 ± 1.0Brix.
The present invention mixes by being allocated with maltodextrin and cycloheptaamylose by concentrated solution before spray drying, and wherein maltodextrin can effectively prevent the material when spraying dry from gluing tower; And the object Main Function allocating cycloheptaamylose is how empty proterties embedding fragrance, the effective constituent that utilize cycloheptaamylose, prevent from losing in high-temperature drying procedures.In further scheme, step 6 of the present invention) in maltodextrin addition accounts for the 20-50% of solid content, cycloheptaamylose accounts for solid content 5-10%.
In further scheme, step 7 of the present invention) spraying dry time, control inlet temperature is 180-210 DEG C; Air outlet temperature is 95-105 DEG C.
Be below specific embodiment of the present invention, raw material adopted in the following embodiments all can be obtained by buying pattern.
embodiment 1:
1) pulverize: by multifunctional crusher by Grosvenor Momordica fresh fruit raw material pulverizing to 8 order;
2) extract: the water-dialyzing adding 8 times 40 DEG C in extractor, add 2% polygalacturonase of raw material proportion, the cellulase of 1%, insulation 90min, ultrasonic synergistic is utilized to extract while enzymolysis, ultrasonic power is at 1000W, and frequency is 25KHZ, and ultrasonic intensity is 5W/cm
2;
3) coarse filtration be separated: cross and filter the impurity of below 200 orders, with 6000 revs/min, the inlet amount of 3.5 tons/hour, disk centrifugal is separated, and gets supernatant liquid;
4) membrane concentration: adopt polyamide composite film, at 30 DEG C of concentrated 120min, discharge concentration is 15.0 ± 1.0Brix, obtains Grosvenor Momordica underflow;
5) allocate: add maltodextrin by 50% of solid content; Cycloheptaamylose is added by 5% of solid content;
6) spraying dry: inlet temperature 180 DEG C; Air outlet temperature: 95 DEG C, Fructus Monordicae extract moisture content is below 5%.
embodiment 2:
1) pulverize: by multifunctional crusher by Grosvenor Momordica fresh fruit raw material pulverizing to 20 order;
2) extract: the water-dialyzing adding 10 times 50 DEG C in extractor, add 5% polygalacturonase of raw material proportion, the cellulase of 3%, insulation 50min, ultrasonic synergistic is utilized to extract while enzymolysis, ultrasonic power is at 500W, and frequency is 20KHZ, and ultrasonic intensity is 2W/cm
2.
3) coarse filtration be separated: cross and filter the impurity of below 200 orders, with 8000 revs/min, the inlet amount of 3.5 tons/hour, disk centrifugal is separated, and gets supernatant liquid;
4) membrane concentration: adopt polyamide composite film, at 40 DEG C of concentrated 90min, discharge concentration is 15.0 ± 1.0Brix, obtains Grosvenor Momordica underflow;
5) allocate: add maltodextrin by 20% of solid content; Cycloheptaamylose is added by 10% of solid content;
6) spraying dry: inlet temperature 210 DEG C; Air outlet temperature: 105 DEG C, Fructus Monordicae extract moisture content is below 5%.
Comparative example 1
According to step and the processing condition of embodiment 2, wherein 2) extraction step is as follows: the water-dialyzing adding 10 times 50 DEG C in extractor, and decocting method extracts 50min; All the other steps are identical with embodiment 2.
Comparative example 2
According to embodiment 2 and processing condition, wherein 2) extraction step is as follows: the water-dialyzing adding 10 times 50 DEG C in extractor, microwave extraction 50min; All the other steps are identical with embodiment 2.
Comparative example 3
According to embodiment 2 and processing condition, wherein 2) extraction step is as follows: the water-dialyzing adding 10 times 50 DEG C in extractor, ultrasonic extraction 50min; All the other steps are identical with embodiment 2.
Compare the content of effective constituent in the yield of above-described embodiment 2 and the last product of comparative example 1-3, extract and the local flavor of extract and extraction cost, comparative result is in table 1.
The comparative result of table 1 embodiment 2 and comparative example 1-3
Note: ☆ represents grade, wherein ☆ more multilist show that more excellent grade, become to produce ☆ in cost more, represent that cost is lower.
The result of table 1 shows that enzymolysis disclosed by the invention, ultrasonic synergistic extract the method preparing Grosvenor Momordica fresh fruit extract, obviously has the advantages such as yield is high, active constituent content is high, flavor taste is good, production cost is low.
Above-mentioned embodiment is only the preferred embodiment of the present invention; can not limit the scope of protection of the invention with this, change and the replacement of any unsubstantiality that those skilled in the art does on basis of the present invention all belong to the present invention's scope required for protection.
Claims (9)
1. enzymolysis-ultrasonic synergistic extracts a method for Grosvenor Momordica fresh fruit extract, and it is characterized in that, the method comprises the following steps:
1) pulverize: select Grosvenor Momordica fresh fruit, to load after adopting pulverizer to pulverize in extractor and to add water-dialyzing;
2) extract: in extractor, add polygalacturonase and cellulase, put into by extractor with ultrasonoscope enzymolysis under the condition of 40-50 DEG C, ultrasonic synergistic extracts 50-90min simultaneously, obtains solidliquid mixture;
3) coarse filtration: by above-mentioned steps 2) in solidliquid mixture double-deck screen shale shaker, removing large granular impurity;
4) centrifugal: through step 3) process after solidliquid mixture dish-style machine centrifugal carry out centrifugal, filter, get liquid;
5) membrane concentration: through step 4) centrifugal after liquid polyamide composite film concentrate;
6) allocate: through step 5) add maltodextrin in enriched material after process and cycloheptaamylose is deployed into mixture;
7) spraying dry: by step 6) mixture carry out spraying dry, control Fructus Monordicae extract moisture content below 5%.
2. method according to claim 1, is characterized in that, step 2) in the weight of polygalacturonase of adding be the 2-5% of Grosvenor Momordica fresh fruit weight, the weight of the cellulase of interpolation is the 1-3% of Grosvenor Momordica fresh fruit weight.
3. method according to claim 1, is characterized in that, step 2) in ultrasonic power be 800-1000W, frequency is 20-25KHZ, and ultrasonic intensity is 2-5W/cm
2.
4. method according to claim 1, is characterized in that, step 3) in, the upper strata mesh screen of double-deck screen shale shaker is 80 orders, and lower floor's mesh screen is 200 orders.
5. method according to claim 1, is characterized in that, step 4) in centrifugal process, the rotating speed of disk centrifugal separator is 6000-8000 rev/min, inlet amount control bit 3.0-3.5 ton/hour.
6. method according to claim 1, is characterized in that, concentration time controls within 120min, thickening temperature≤40 DEG C.
7. method according to claim 6, is characterized in that, step 5) in the discharge concentration of concentrated solution be 15.0 ± 1.0Brix.
8. method according to claim 1, is characterized in that, step 6) in maltodextrin addition accounts for the 20-50% of solid content, cycloheptaamylose accounts for solid content 5-10%.
9. method according to claim 1, is characterized in that, step 7) spraying dry time, control inlet temperature is 180-210 DEG C; Air outlet temperature is 95-105 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510752134.3A CN105255946A (en) | 2015-11-06 | 2015-11-06 | Method for extracting fresh fructus momordicae fruit extract by virtue of enzymolysis-ultrasonic wave cooperation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510752134.3A CN105255946A (en) | 2015-11-06 | 2015-11-06 | Method for extracting fresh fructus momordicae fruit extract by virtue of enzymolysis-ultrasonic wave cooperation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105255946A true CN105255946A (en) | 2016-01-20 |
Family
ID=55095873
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510752134.3A Pending CN105255946A (en) | 2015-11-06 | 2015-11-06 | Method for extracting fresh fructus momordicae fruit extract by virtue of enzymolysis-ultrasonic wave cooperation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105255946A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113383836A (en) * | 2021-05-06 | 2021-09-14 | 湖南绿蔓生物科技股份有限公司 | Low-sugar milk tea and preparation method thereof |
| CN113455550A (en) * | 2021-06-30 | 2021-10-01 | 湖南绿蔓生物科技股份有限公司 | Preparation method of momordica grosvenori bean curd |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1683387A (en) * | 2005-03-16 | 2005-10-19 | 桂林莱茵生物科技股份有限公司 | Method for extracting momordica glycoside from momordica grosvenori |
| CN101029320A (en) * | 2007-04-09 | 2007-09-05 | 重庆工学院 | Method for extracting bean mill cake isoflavone by cellulase synergistic supersonic wave |
| CN101177444A (en) * | 2006-11-11 | 2008-05-14 | 大闽食品（漳州）有限公司 | Method for extracting momordica grosvenori flavone glycoside from momordica grosvenori |
| CN102356865A (en) * | 2011-09-19 | 2012-02-22 | 桂林莱茵生物科技股份有限公司 | Sweetener and preparation method thereof |
| CN103689158A (en) * | 2013-11-21 | 2014-04-02 | 深圳市深宝实业股份有限公司 | Preparation method of chrysanthemum morifolium extract |
| CN103923152A (en) * | 2014-05-08 | 2014-07-16 | 济南汉定生物工程有限公司 | Method for extracting mogroside V |
-
2015
- 2015-11-06 CN CN201510752134.3A patent/CN105255946A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1683387A (en) * | 2005-03-16 | 2005-10-19 | 桂林莱茵生物科技股份有限公司 | Method for extracting momordica glycoside from momordica grosvenori |
| CN101177444A (en) * | 2006-11-11 | 2008-05-14 | 大闽食品（漳州）有限公司 | Method for extracting momordica grosvenori flavone glycoside from momordica grosvenori |
| CN101029320A (en) * | 2007-04-09 | 2007-09-05 | 重庆工学院 | Method for extracting bean mill cake isoflavone by cellulase synergistic supersonic wave |
| CN102356865A (en) * | 2011-09-19 | 2012-02-22 | 桂林莱茵生物科技股份有限公司 | Sweetener and preparation method thereof |
| CN103689158A (en) * | 2013-11-21 | 2014-04-02 | 深圳市深宝实业股份有限公司 | Preparation method of chrysanthemum morifolium extract |
| CN103923152A (en) * | 2014-05-08 | 2014-07-16 | 济南汉定生物工程有限公司 | Method for extracting mogroside V |
Non-Patent Citations (2)
| Title |
|---|
| 李俊 等: "超声波法提取罗汉果多糖的工艺研究", 《中药材》 * |
| 李洪燕 等: "酶解法提取罗汉果多糖的工艺研究", 《食品安全质量检测学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113383836A (en) * | 2021-05-06 | 2021-09-14 | 湖南绿蔓生物科技股份有限公司 | Low-sugar milk tea and preparation method thereof |
| CN113383836B (en) * | 2021-05-06 | 2023-02-28 | 湖南绿蔓生物科技股份有限公司 | Low-sugar milk tea and preparation method thereof |
| CN113455550A (en) * | 2021-06-30 | 2021-10-01 | 湖南绿蔓生物科技股份有限公司 | Preparation method of momordica grosvenori bean curd |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101073370A (en) | Blackthorn pear products and its production | |
| CN106243172B (en) | A method of extracting black fruit fructus lycii anthocyanin | |
| CN105669800B (en) | A kind of combined extracting essential oil from citrus, pectin, aurantiamarin, the method for synephrine and limonin | |
| CN102106928B (en) | Method for preparing high-purity oil tea saponins | |
| CN101019899A (en) | Buccal tablet of aweto or aweto extract | |
| CN105996028B (en) | Ginger extract, ginger fiber and preparation method of ginger extract and ginger fiber | |
| CN102304501A (en) | Complex enzyme preparation and application and method thereof for extracting plant polysaccharides by using same | |
| CN107163098A (en) | A kind of extracting method of natural high-purity mogroside | |
| CN102675398B (en) | A kind of method extracting momordica grosvenori glycoside V and farnesol from Grosvenor Momordica | |
| CN103168876A (en) | Medlar black tea | |
| CN105061631A (en) | Extraction method and application of taraxacum polysaccharide | |
| CN105255946A (en) | Method for extracting fresh fructus momordicae fruit extract by virtue of enzymolysis-ultrasonic wave cooperation | |
| CN105732741A (en) | Method for extracting anthocyanin and ursolic acid from perilla leaves | |
| CN101696381B (en) | Novel process for preparing highland barley flavone extract and application thereof in health wine | |
| CN109021042B (en) | Method for extracting high-purity oleuropein from olive leaves | |
| CN104230871B (en) | A kind of method separating polymethoxyflavone in Fructus Aurantii Immaturus, Hesperidin and Neosynephrine | |
| CN104945450A (en) | Method for extracting stibene glucoside from vines of multiflower knotweeds | |
| CN104739916A (en) | Alpha-glucosidase active inhibitor as well as preparation method and application thereof | |
| CN102727540A (en) | Method for preparing red bayberry leaf extract | |
| CN104095177A (en) | Health-care granule by using ginseng flower bud as the main raw material and preparation method thereof | |
| CN103859398A (en) | Health-care food with functions of lowering blood glucose and improving eyesight and preparation method of food | |
| CN102430006B (en) | Method for extracting purified polyphenol from young fruits of lycium chinense | |
| CN102648750A (en) | Refine Chinese date cane sugar, Chinese date high fructose syrup and preparation method of refine Chinese date cane sugar and Chinese date high fructose syrup | |
| CN103053996A (en) | Plant salt and preparation method thereof | |
| CN102690359A (en) | Method for extracting starch and cucurbitacins from siraitia grosvenorii roots |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160120 |