CN111187363A - Extraction method of tremella polysaccharide - Google Patents
Extraction method of tremella polysaccharide Download PDFInfo
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- CN111187363A CN111187363A CN201811356044.2A CN201811356044A CN111187363A CN 111187363 A CN111187363 A CN 111187363A CN 201811356044 A CN201811356044 A CN 201811356044A CN 111187363 A CN111187363 A CN 111187363A
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- tremella
- tremella polysaccharide
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Organic Chemistry (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention discloses a method for extracting tremella polysaccharide, which is simple and convenient to operate and high in extraction efficiency. The processing steps comprise: weighing 15-25 g of crushed tremella powder, uniformly dividing into 3 parts, respectively putting into 80ml beakers, adding water to 60ml, putting into an electric heating constant-temperature water bath kettle, performing suction filtration by using a circulating water type multipurpose vacuum pump after about 3 hours at 65 ℃, and putting the filtrate into another 250ml big beaker; extracting with the same method for 3 times; and combining the filtrates for 3 times, heating and concentrating by using an electrothermal constant-temperature water bath kettle for 10-20 ml, cooling to room temperature, adding 60ml of ethanol, standing at a backlight position, performing suction filtration by using a gauze funnel, and washing the filter cake for 3 times by using ethanol and diethyl ether in sequence to obtain the tremella polysaccharide. The tremella polysaccharide prepared by the method has high purity, and is easy for industrial production.
Description
Technical Field
The invention belongs to the field of natural extracts, and particularly relates to a method for extracting tremella polysaccharide.
Background
Tremella is also called as Tremella, etc., belongs to Tremella of Tremellaceae, and is the fruiting body of Tremella of Basidiomycota, and has the name of "crown of fungus". Tremella fuciformis is sweet and light in taste, mild in nature and nontoxic, has the effects of tonifying spleen and promoting appetite, and also has the effects of benefiting qi, clearing intestines, nourishing yin and moistening lung. It can not only enhance immunity, but also enhance tolerance of tumor patients to radiotherapy and chemotherapy. Tremella is rich in natural plant colloid, has effects of nourishing yin, and can be used as skin caring food for long-term administration.
White fungus originates from Sichuan Tongjiang, and the Qinggong wai girl drafts' book Yuxiang Shu Jiang Li from Yu Xiang Liu, Yu Shang House: "Tremella fuciformis is very expensive in market price, and one small box of Tremella fuciformis can be bought only by spending one or twenty-two silvers.
Tremella polysaccharide (Tremellam) is an acidic heteropolysaccharide, is a basidiomycete polysaccharide immunopotentiator, and has functions of improving organism immunity and increasing leukocyte. Can significantly improve the phagocytic function of reticuloendothelial cells of experimental animals, promote nonspecific immunity, and improve the level of immunoglobulin and serum total complement. Can be used for preventing and treating bone marrow depression caused by radiation and antineoplastic agent (such as CTX), and promoting synthesis of protein in liver. In addition, the traditional Chinese medicine composition can also be used for treating chronic bronchitis, and the effective rate is up to more than 80%.
The immune function of the tremella polysaccharide is mainly focused on two aspects, namely aiming at a non-immune system, promoting the growth of beneficial microorganisms of the gastrointestinal tract, regulating the formation of an ideal microbial flora of the intestinal tract and enhancing the resistance of animals to exogenous pathogenic bacteria; secondly, aiming at an immune defense system, the humoral immunity is improved, and the phagocytic capacity of phagocyte is enhanced; improve the activity and function of lymphocytes, promote the growth of cytokines and protect erythrocyte membranes from being easily damaged by oxidation. Thereby improving the autoimmune capability of the animal body and enhancing the resistance of the animal to diseases. Besides the function of improving the immunity of the organism, the tremella polysaccharide can also promote the synthesis of protein and nucleic acid, increase the self-repairing capability and maintain the functions of various organs and tissues, particularly the liver.
Disclosure of Invention
In order to meet clinical requirements and expand the variety of medicines, the tremella polysaccharide is applied to prepare the medicines for invigorating stomach and regulating qi.
The technical scheme of the invention is carried out according to the following steps:
a. weighing 15-25 g of crushed tremella powder, uniformly dividing into 3 parts, respectively putting into 80ml beakers, and adding water to 60 ml. (ii) a
b. Placing in an electric heating constant temperature water bath kettle, performing suction filtration at 65 deg.C for 3 hr, and placing the filtrate in another 250ml big beaker;
c. extracting with the same method for 3 times;
d. and combining the filtrates for 3 times, heating and concentrating by using an electrothermal constant-temperature water bath kettle for 10-20 ml, cooling to room temperature, adding 60ml of ethanol, standing at a backlight position, performing suction filtration by using a gauze funnel, and washing the filter cake for 3 times by using ethanol and diethyl ether in sequence to obtain the tremella polysaccharide.
The water added in the step a to 60ml is distilled water.
And d, standing for 24h at the backlight in the step d.
The present invention will be further described with reference to specific embodiments, but the scope of the invention as claimed is not limited to the following embodiments.
Detailed Description
Example 1:
weighing 15g of crushed tremella powder, uniformly dividing into 3 parts, respectively putting into 80ml beakers, and adding water to 60 ml. Placing in an electric heating constant temperature water bath kettle, performing suction filtration at 65 deg.C for 3 hr, and placing the filtrate in another 250ml big beaker. The extraction was repeated 3 times in the same manner. Mixing the filtrates, heating and concentrating with electrothermal constant temperature water bath kettle for 10ml, cooling to room temperature, adding 60ml ethanol, standing in backlight, vacuum filtering with gauze funnel, and washing the filter cake with ethanol and diethyl ether for 3 times to obtain Tremella polysaccharide 0.478 g.
Example 2:
weighing 25g of crushed tremella powder, uniformly dividing into 3 parts, respectively putting into 80ml beakers, and adding water to 60 ml. Placing in an electric heating constant temperature water bath kettle, performing suction filtration at 65 deg.C for 3 hr, and placing the filtrate in another 250ml big beaker. The extraction was repeated 3 times in the same manner. Mixing the filtrates, heating and concentrating with electrothermal constant temperature water bath kettle for 10ml, cooling to room temperature, adding 60ml ethanol, standing in backlight, vacuum filtering with gauze funnel, and washing the filter cake with ethanol and diethyl ether for 3 times to obtain Tremella polysaccharide 0.712 g.
Example 3:
weighing 25g of crushed tremella powder, uniformly dividing into 3 parts, respectively putting into 80ml beakers, and adding water to 60 ml. Placing in an electric heating constant temperature water bath kettle, performing suction filtration at 65 deg.C for 3 hr, and placing the filtrate in another 250ml big beaker. The extraction was repeated 3 times in the same manner. Mixing the filtrates, heating and concentrating with electrothermal constant temperature water bath kettle for 20ml, cooling to room temperature, adding 60ml ethanol, standing in backlight, vacuum filtering with gauze funnel, and washing the filter cake with ethanol and diethyl ether for 3 times to obtain Tremella polysaccharide 0.752 g.
Claims (3)
1. The extraction method of the tremella polysaccharide is characterized by comprising the following steps:
a. weighing 15-25 g of crushed tremella powder, uniformly dividing into 3 parts, respectively putting into 80ml beakers, and adding water to 60ml;
b. placing in an electric heating constant temperature water bath kettle, performing suction filtration at 65 deg.C for 3 hr, and placing the filtrate in another 250ml big beaker;
c. extracting with the same method for 3 times;
d. and combining the filtrates for 3 times, heating and concentrating by using an electrothermal constant-temperature water bath kettle for 10-20 ml, cooling to room temperature, adding 60ml of ethanol, standing at a backlight position, performing suction filtration by using a gauze funnel, and washing the filter cake for 3 times by using ethanol and diethyl ether in sequence to obtain the tremella polysaccharide.
2. The method for extracting tremella polysaccharide as claimed in claim 1, wherein the water added to 60ml in step a is distilled water.
3. The method for extracting Tremella polysaccharides as claimed in claim 1, wherein the step d is performed by standing at a backlight for 24 h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811356044.2A CN111187363A (en) | 2018-11-15 | 2018-11-15 | Extraction method of tremella polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811356044.2A CN111187363A (en) | 2018-11-15 | 2018-11-15 | Extraction method of tremella polysaccharide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111187363A true CN111187363A (en) | 2020-05-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811356044.2A Pending CN111187363A (en) | 2018-11-15 | 2018-11-15 | Extraction method of tremella polysaccharide |
Country Status (1)
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|---|---|
| CN (1) | CN111187363A (en) |
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2018
- 2018-11-15 CN CN201811356044.2A patent/CN111187363A/en active Pending
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20200522 |