CN111184815A - Anti-tumor injection for treating lymphosarcoma and preparation method thereof - Google Patents
Anti-tumor injection for treating lymphosarcoma and preparation method thereof Download PDFInfo
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- CN111184815A CN111184815A CN202010113249.9A CN202010113249A CN111184815A CN 111184815 A CN111184815 A CN 111184815A CN 202010113249 A CN202010113249 A CN 202010113249A CN 111184815 A CN111184815 A CN 111184815A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/074—Ganoderma
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/51—Gentianaceae (Gentian family)
- A61K36/515—Gentiana
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/66—Papaveraceae (Poppy family), e.g. bloodroot
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8969—Polygonatum (Solomon's seal)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/08—Solutions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
Abstract
The invention discloses an anti-tumor injection for treating lymphosarcoma and a preparation method thereof, wherein the injection comprises the following components by weight: 40-60 parts of lucid ganoderma, 30-50 parts of rhizoma polygonati, 10-20 parts of muskroot-like semiaquilegia root, 8-16 parts of corydalis amabilis, 5-15 parts of gentiana macrophylla, 3-10 parts of ternate buttercup root, 6-16 parts of Indian iphigenia bulb and 120-160 parts of injection; in the preparation process, extracting effective components from the components to obtain solid coagulum, dissolving the solid coagulum in injection liquid, and sterilizing and packaging to obtain the injection liquid of the finished product; the injection provided by the invention can directly enter blood vessels through injection, has the characteristics of easy absorption and quick response, can effectively improve the immunity of patients, and can inhibit the growth of lymphosarcoma; meanwhile, the materials selected for the liquid medicine are harmless raw materials, so that adverse reaction on patients is avoided; meanwhile, the preparation process of the liquid medicine is simple, the operability is strong, and the method is suitable for mass production and processing.
Description
Technical Field
The invention relates to the technical field of medical drugs, in particular to an anti-tumor injection for treating lymphosarcoma and a preparation method thereof.
Background
Lymphosarcoma is one of the old classifications, corresponding to the non-hodgkin's lymphoma of good and bad lymphocyte differentiation in the non-hodgkin's lymphoma classification, and it grows fast and voluminous, mostly affecting one or two lymph nodes. It usually occurs in the cervical lymph nodes, and then in the axilla, groin and mediastinum. Lymphosarcoma may also first occur in the gastrointestinal tract, five sense organs, mediastinum, or skin. After originating in an organ, it is transferred to other sites. In the peripheral blood at an advanced stage, a large number of pathological cells, called white blood sarcoma, sometimes appear.
The characteristics of cancer cells are: unlimited and endless hyperplasia can lead to the nutrient substances in the body of a patient to be greatly consumed, the cancer cells release various toxins, the human body generates a series of symptoms, and the cancer cells can also be transferred to all parts of the whole body to grow and reproduce, thus leading to the emaciation, the weakness, the anemia, the inappetence, the fever, the serious organ function damage and the like of the human body.
At present, there are many means and methods for treating malignant tumor, such as surgical operation, chemical drug therapy, radiotherapy, immunotherapy and the like, and these treatment means have certain curative effect on malignant tumor, but have serious adverse reaction and the defects of limited curative effect and the like.
Disclosure of Invention
The invention aims to provide an anti-tumor injection for treating lymphosarcoma and a preparation method thereof, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
an anti-tumor injection for treating lymphosarcoma and a preparation method thereof, which comprises the following components by weight: 40-60 parts of lucid ganoderma, 30-50 parts of rhizoma polygonati, 10-20 parts of muskroot-like semiaquilegia root, 8-16 parts of corydalis amabilis, 5-15 parts of gentiana macrophylla, 3-10 parts of ternate buttercup root, 6-16 parts of Indian iphigenia bulb and 120-160 parts of injection.
According to a preferable technical scheme, the injection comprises, by weight, 48-55 parts of lucid ganoderma, 36-48 parts of rhizoma polygonati, 12-18 parts of radix semiaquilegiae, 10-14 parts of rhizoma corydalis decumbentis, 8-13 parts of gentiana macrophylla, 5-8 parts of ternate buttercup root, 9-15 parts of edible tulip and 125-140 parts of injection.
As a preferred technical scheme, the invention also provides a preparation method of the antitumor injection for treating the lymphosarcoma, which comprises the following steps:
s1, weighing the raw materials according to the weight proportion, and cleaning and drying the raw materials;
s2, extracting effective components from the raw materials to respectively obtain coagulates of the raw materials;
s3, drying the coagulum prepared in the step S2 to obtain a lump, and crushing the lump to obtain a powder raw material;
s4, adding the injection liquid into the powder raw material obtained in the step S3, heating and boiling the mixture, and then filtering the liquid to obtain filtrate;
s5, sterilizing and packaging the filtrate to obtain the injection.
As a preferred technical scheme of the present invention, in the step S1, the drying temperature is 85 to 95 ℃, and the drying time is 30 to 60 min.
As a preferred technical scheme of the present invention, in step S2, the extraction process of the ganoderma lucidum and the muskroot-like semiaquilegia root includes placing the ganoderma lucidum and the muskroot-like semiaquilegia root in 5-8 times of 80% ethanol, soaking for 4-6 hours, taking out the ganoderma lucidum, and recovering the ethanol through evaporation to obtain the ganoderma lucidum extract.
As a preferred technical scheme of the present invention, in step S2, the rhizoma polygonati and large-leaved gentian are extracted by mixing and crushing rhizoma polygonati and large-leaved gentian, adding the crushed powder into purified water, decocting, filtering the dregs of a decoction to obtain a liquid medicine, and concentrating the liquid medicine to obtain a fluid extract.
As a preferred technical solution of the present invention, in the step S2, the corydalis amabilis, ternate buttercup root and edible tulip are extracted by crushing the materials, adding purified water to soak the crushed materials, filtering the dregs to obtain a liquid medicine, and concentrating the liquid medicine to obtain the clear paste.
In a preferred technical scheme of the invention, in the step S3, the extract is dried at 105-115 ℃ for not less than 30min, and is screened by using a 60-100 mesh screen after being crushed.
In a preferred embodiment of the present invention, in step S4, the powder material is added to 1/3 of the injection solution, boiled for 1-2 hours, filtered, the remaining injection solution is added to the filtrate, boiled for not less than 1.5 hours, and filtered to obtain the liquid medicine.
In a preferred embodiment of the present invention, in step S5, the injection is obtained by sterilizing the drug solution with ultraviolet rays and then packaging the sterilized drug solution.
Compared with the prior art, the invention has the beneficial effects that: the injection provided by the invention can directly enter blood vessels through injection, has the characteristics of easy absorption and quick response, can effectively improve the immunity of patients, and can inhibit the growth of lymphosarcoma; meanwhile, the materials selected for the liquid medicine are harmless raw materials, so that adverse reaction on patients is avoided; meanwhile, the preparation process of the liquid medicine is simple, the operability is strong, and the method is suitable for mass production and processing.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
an anti-tumor injection for treating lymphosarcoma, which comprises the following components by weight: 52 parts of lucid ganoderma, 42 parts of rhizoma polygonati, 16 parts of muskroot-like semiaquilegia root, 12 parts of corydalis decumbens, 10 parts of gentiana macrophylla, 6 parts of radix ranunculi ternati, 10 parts of edible tulip and 140 parts of injection.
The preparation method comprises the following steps:
the preparation process of antitumor injection for treating lymphosarcoma includes the following steps:
s1, weighing the raw materials according to the weight proportion, cleaning and drying at the drying temperature of 90 ℃ for 45 min;
s2, extracting effective components from the raw materials to respectively obtain coagulates of the raw materials; wherein the extraction process of Ganoderma and radix Semiaquilegiae comprises soaking Ganoderma and radix Semiaquilegiae in 6 times of 80% ethanol for 5 hr, taking out Ganoderma, and recovering ethanol by evaporation to obtain Ganoderma extract; the extraction process of rhizoma Polygonati and radix Gentianae Marcrophyllae comprises mixing rhizoma Polygonati and radix Gentianae Marcrophyllae, pulverizing, adding the pulverized powder into purified water, decocting, filtering the residue to obtain medicinal liquid, and concentrating the medicinal liquid to obtain fluid extract; the extraction process of rhizoma corydalis Decumbentis, radix Ranunculi Ternati and Pseudobulbus Cremastrae Seu pleiones comprises pulverizing the materials, soaking in purified water, filtering to remove residues to obtain medicinal liquid, and concentrating the medicinal liquid to obtain fluid extract;
s3, drying the coagulum prepared in the step S2 at the drying temperature of 110 ℃ for 45min to obtain a lump, crushing the lump, and screening by using a 80-mesh screen to obtain a powder raw material;
s4, adding the powder material into 1/3 of the injection, boiling for 1.5h, filtering, adding the filtrate into the rest of the injection, boiling for 2.5h, and filtering to obtain a liquid medicine;
s5, sterilizing the filtrate with ultraviolet rays, and packaging to obtain the injection.
Example two:
an anti-tumor injection for treating lymphosarcoma, which comprises the following components by weight: 42 parts of lucid ganoderma, 32 parts of rhizoma polygonati, 11 parts of muskroot-like semiaquilegia root, 9 parts of corydalis decumbens, 6 parts of gentiana macrophylla, 3 parts of radix ranunculi ternati, 7 parts of edible tulip and 125 parts of injection.
The preparation method comprises the following steps:
the preparation process of antitumor injection for treating lymphosarcoma includes the following steps:
s1, weighing the raw materials according to the weight proportion, cleaning and drying at 86 ℃ for 30 min;
s2, extracting effective components from the raw materials to respectively obtain coagulates of the raw materials; wherein the extraction process of Ganoderma and radix Semiaquilegiae comprises soaking Ganoderma and radix Semiaquilegiae in 5 times of 80% ethanol for 4.2 hr, taking out Ganoderma, and recovering ethanol by evaporation to obtain Ganoderma extract; the extraction process of rhizoma Polygonati and radix Gentianae Marcrophyllae comprises mixing rhizoma Polygonati and radix Gentianae Marcrophyllae, pulverizing, adding the pulverized powder into purified water, decocting, filtering the residue to obtain medicinal liquid, and concentrating the medicinal liquid to obtain fluid extract; the extraction process of rhizoma corydalis Decumbentis, radix Ranunculi Ternati and Pseudobulbus Cremastrae Seu pleiones comprises pulverizing the materials, soaking in purified water, filtering to remove residues to obtain medicinal liquid, and concentrating the medicinal liquid to obtain fluid extract;
s3, drying the coagulum prepared in the step S2 at 105 ℃ for 30min to obtain a lump, crushing the lump, and screening by using a 60-mesh screen to obtain a powder raw material;
s4, adding the powder material into 1/3 of the injection, boiling for 1.2h, filtering, adding the filtrate into the rest of the injection, boiling for 1.5h, and filtering to obtain a liquid medicine;
s5, sterilizing the filtrate with ultraviolet rays, and packaging to obtain the injection.
Example three:
an anti-tumor injection for treating lymphosarcoma, which comprises the following components by weight: 55 parts of lucid ganoderma, 48 parts of rhizoma polygonati, 18 parts of muskroot-like semiaquilegia root, 15 parts of corydalis decumbens, 15 parts of gentiana macrophylla, 9 parts of radix ranunculi ternati, 16 parts of edible tulip and 150 parts of injection.
The preparation method comprises the following steps:
the preparation process of antitumor injection for treating lymphosarcoma includes the following steps:
s1, weighing the raw materials according to the weight proportion, cleaning and drying at the drying temperature of 95 ℃ for 50 min;
s2, extracting effective components from the raw materials to respectively obtain coagulates of the raw materials; wherein the extraction process of Ganoderma and radix Semiaquilegiae comprises soaking Ganoderma and radix Semiaquilegiae in 7 times of 80% ethanol for 5.6 hr, taking out Ganoderma, and recovering ethanol by evaporation to obtain Ganoderma extract; the extraction process of rhizoma Polygonati and radix Gentianae Marcrophyllae comprises mixing rhizoma Polygonati and radix Gentianae Marcrophyllae, pulverizing, adding the pulverized powder into purified water, decocting, filtering the residue to obtain medicinal liquid, and concentrating the medicinal liquid to obtain fluid extract; the extraction process of rhizoma corydalis Decumbentis, radix Ranunculi Ternati and Pseudobulbus Cremastrae Seu pleiones comprises pulverizing the materials, soaking in purified water, filtering to remove residues to obtain medicinal liquid, and concentrating the medicinal liquid to obtain fluid extract;
s3, drying the coagulum prepared in the step S2 at 112 ℃ for 90min to obtain a lump, crushing the lump, and screening by using a 100-mesh screen to obtain a powder raw material;
s4, adding the powder material into 1/3 of injection liquid, boiling for 2 hours, filtering, adding the filtrate into the rest of injection liquid, boiling for 2 hours, and filtering to obtain a liquid medicine;
s5, sterilizing the filtrate with ultraviolet rays, and packaging to obtain the injection.
The difference between the first embodiment, the second embodiment and the third embodiment is that the specific gravity of the raw material components and the parameters in the preparation method steps are different.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. An anti-tumor injection for treating lymphosarcoma, which is characterized in that: the composition consists of the following components in parts by weight: 40-60 parts of lucid ganoderma, 30-50 parts of rhizoma polygonati, 10-20 parts of muskroot-like semiaquilegia root, 8-16 parts of corydalis amabilis, 5-15 parts of gentiana macrophylla, 3-10 parts of ternate buttercup root, 6-16 parts of Indian iphigenia bulb and 120-160 parts of injection.
2. The liquid injection for treating lymphosarcoma of claim 1, wherein: the injection comprises, by weight, 48-55 parts of lucid ganoderma, 36-48 parts of rhizoma polygonati, 12-18 parts of muskroot-like semiaquilegia root, 10-14 parts of corydalis amabilis, 8-13 parts of gentiana macrophylla, 5-8 parts of ternate buttercup root, 9-15 parts of edible tulip and 125-140 parts of injection.
3. The method for preparing an anti-tumor injection for treating lymphosarcoma according to any one of claims 1-2, comprising the steps of:
s1, weighing the raw materials according to the weight proportion, and cleaning and drying the raw materials;
s2, extracting effective components from the raw materials to respectively obtain coagulates of the raw materials;
s3, drying the coagulum prepared in the step S2 to obtain a lump, and crushing the lump to obtain a powder raw material;
s4, adding the injection liquid into the powder raw material obtained in the step S3, heating and boiling the mixture, and then filtering the liquid to obtain filtrate;
s5, sterilizing and packaging the filtrate to obtain the injection.
4. The method for preparing an anti-tumor injection for treating lymphosarcoma according to claim 3, wherein: in the step S1, the drying temperature is 85-95 ℃, and the drying time is 30-60 min.
5. The method for preparing an anti-tumor injection for treating lymphosarcoma according to claim 3, wherein: in the step S2, the extraction process of the ganoderma lucidum and the muskroot-like semiaquilegia root comprises the steps of placing the ganoderma lucidum and the muskroot-like semiaquilegia root in 5-8 times of 80% ethanol, soaking for 4-6 hours, taking out the ganoderma lucidum, and recovering the ethanol through evaporation to obtain the ganoderma lucidum extract.
6. The method for preparing an anti-tumor injection for treating lymphosarcoma according to claim 3, wherein: in the step S2, the rhizoma polygonati and large-leaved gentian are extracted by mixing and crushing the rhizoma polygonati and the large-leaved gentian, adding the crushed powder into purified water for decoction, filtering the dregs of a decoction to obtain a liquid medicine, and concentrating the liquid medicine to obtain the clear paste.
7. The method for preparing an anti-tumor injection for treating lymphosarcoma according to claim 3, wherein: in the step S2, the extraction process of the corydalis amabilis, the ternate buttercup root and the edible tulip comprises the steps of crushing the materials, adding purified water for soaking after crushing, finally filtering the dregs of a decoction to obtain a liquid medicine, and concentrating the liquid medicine to obtain the clear paste.
8. The method for preparing an anti-tumor injection for treating lymphosarcoma according to claim 3, wherein: in the step S3, the extract is dried at 105-115 ℃ for not less than 30min, and is screened by using a 60-100 mesh screen after being crushed.
9. The method for preparing an anti-tumor injection for treating lymphosarcoma according to claim 3, wherein: in the step S4, the powder material is added into 1/3 of the injection solution, the mixture is boiled for 1-2 hours and then filtered, the remaining injection solution is added into the filtrate, the mixture is boiled for not less than 1.5 hours, and then the mixture is filtered to obtain the liquid medicine.
10. The method for preparing an anti-tumor injection for treating lymphosarcoma according to claim 3, wherein: in step S5, the liquid medicine is sterilized with ultraviolet rays and then packaged to obtain an injection.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010113249.9A CN111184815A (en) | 2020-02-24 | 2020-02-24 | Anti-tumor injection for treating lymphosarcoma and preparation method thereof |
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| CN202010113249.9A CN111184815A (en) | 2020-02-24 | 2020-02-24 | Anti-tumor injection for treating lymphosarcoma and preparation method thereof |
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Citations (4)
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| CN103330781A (en) * | 2013-06-05 | 2013-10-02 | 西安医学院 | Traditional Chinese medicine composite with anti-tumor effect and preparation method of injection of same |
| CN104147355A (en) * | 2014-07-31 | 2014-11-19 | 朴认南 | Anti-tumor injection agent and preparation method thereof |
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2020
- 2020-02-24 CN CN202010113249.9A patent/CN111184815A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1879842A (en) * | 2006-05-17 | 2006-12-20 | 郭富红 | A Chinese medicine for treating cancer |
| CN101850032A (en) * | 2010-06-08 | 2010-10-06 | 常熟雷允上制药有限公司 | Anti-tumor traditional Chinese medicine composition and preparation method and application thereof |
| CN103330781A (en) * | 2013-06-05 | 2013-10-02 | 西安医学院 | Traditional Chinese medicine composite with anti-tumor effect and preparation method of injection of same |
| CN104147355A (en) * | 2014-07-31 | 2014-11-19 | 朴认南 | Anti-tumor injection agent and preparation method thereof |
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| Title |
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| 陈铁汉等: "中西医结合治疗恶性淋巴瘤44例临床观察", 《湖南中医杂志》 * |
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Application publication date: 20200522 |