CN111184129A - Direct-feed drinking water probiotic and application thereof - Google Patents

Direct-feed drinking water probiotic and application thereof Download PDF

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Publication number
CN111184129A
CN111184129A CN201910698001.0A CN201910698001A CN111184129A CN 111184129 A CN111184129 A CN 111184129A CN 201910698001 A CN201910698001 A CN 201910698001A CN 111184129 A CN111184129 A CN 111184129A
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weight
powder
direct
drinking water
feed
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蔡学敏
查宇
贺小兰
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Hubei Languzhong Microbial Technology Co Ltd
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Hubei Languzhong Microbial Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/28—Silicates, e.g. perlites, zeolites or bentonites

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Sustainable Development (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Inorganic Chemistry (AREA)
  • Fodder In General (AREA)

Abstract

The invention discloses a direct-feed drinking water probiotic agent. The lactobacillus casei strain comprises strain powder and auxiliary materials, wherein the strain powder comprises 0.2-0.5 weight part of lactobacillus casei, 0.2-0.5 weight part of lactobacillus plantarum and 0.1-0.3 weight part of yeast, and the auxiliary materials comprise 30-60 weight parts of glucose, 10-20 weight parts of yeast extract powder, 10-20 weight parts of peptone and 20-30 weight parts of talcum powder. The invention solves the problem of loss of probiotics in feed processing, greatly improves the intake amount of the probiotics for livestock and poultry, has wide application range, can be implemented in feed factories or breeding sites, has low requirement on fields and occupies small area; the operation is simple, convenient and quick, the investment cost is low, the requirement on the professional skills of operators is low, and the device is suitable for most farmers and can be used by small scattered households.

Description

Direct-feed drinking water probiotic and application thereof
Technical Field
The invention relates to the technical field of microbial fermentation, in particular to a direct-feed drinking water probiotic.
Background
Probiotics and microbial fermented feed have recently gained wide attention as the most effective way to replace antibiotics at present, and the way to use probiotics is currently: (1) mixing probiotic powder with feed raw materials, processing and feeding; (2) the probiotics is inoculated into the feed raw material for fermentation and then fed. The problems with both approaches are: the feed processing process has a high-temperature stage, most of probiotics, especially lactobacillus, can not resist high temperature, and the content of the probiotics in the processed feed is extremely low. Meanwhile, the fermented feed is used as a large engineering project, the investment is large, and the operation is complex.
The conventional mode comprises mixing probiotic powder and feed raw materials, processing and feeding, and inoculating probiotic into the feed raw materials for fermentation and feeding. However, the same problems exist with both of these approaches: the feed processing process has a high-temperature stage, most of probiotics, especially lactobacillus, can not resist high temperature, and the content of the probiotics in the processed feed is extremely low. And must be done at the feed mill.
Disclosure of Invention
The invention aims to provide a drinking water probiotic capable of being directly thrown into feed.
In order to achieve the purpose, the invention provides a direct-feed drinking water probiotic preparation, which comprises bacterial powder and auxiliary materials, wherein the bacterial powder comprises 0.2-0.5 part by weight of lactobacillus casei, 0.2-0.5 part by weight of lactobacillus plantarum and 0.1-0.3 part by weight of yeast, and the auxiliary materials comprise 30-60 parts by weight of glucose, 10-20 parts by weight of yeast extract powder, 10-20 parts by weight of peptone and 20-30 parts by weight of talcum powder.
Further, the composition comprises the following components: 0.4 part by weight of lactobacillus casei, 0.4 part by weight of lactobacillus plantarum, 0.2 part by weight of yeast, 50 parts by weight of glucose, 12.5 parts by weight of yeast extract powder, 12.5 parts by weight of peptone and 25 parts by weight of talcum powder.
Further, the yeast accounts for 20% of the total flora weight; the weight ratio of the lactobacillus casei to the lactobacillus plantarum is 1: 1. Wherein the optimal proportion of the microzyme is 20 percent of the total flora, and when the optimal proportion of the microzyme is lower than the value, the risk of mixed bacteria pollution exists in the using process; above this value, yeast grows vigorously and lactobacillus casei and lactobacillus plantarum grow restrictively. The optimal ratio of lactobacillus casei to lactobacillus plantarum is 1; 1, when the ratio is higher than the above range, the acidity of the obtained fermentation liquid is low, and when the ratio is lower than the above range, the content of flavor substances in the fermentation liquid is low.
Furthermore, in the auxiliary materials, the talcum powder accounts for 1/4 of the total weight; the weight ratio of glucose to peptone is 4: 1; 1/8 yeast extract powder is used as main material; more preferably, the weight ratio of the glucose to the yeast extract powder to the peptone to the talc is 2:0.5:0.5: 1. Wherein the talcum powder can prevent the mixed materials from caking, and the most preferred proportion of the talcum powder is 1/4 of the total materials; the optimal ratio of glucose to peptone is 4:1, which is lower than the optimal ratio, the nitrogen source is higher, and the fermentation broth has the risk of bacterial contamination and odor generation; the carbon source is higher than the optimal proportion, and the fermentation liquor alcohol accumulation is increased to influence the taste; the optimal proportion of the yeast extract powder in the materials is 1/8, and when the proportion is lower than the optimal proportion, trace elements required by thalli are insufficient, and the concentration of bacteria in the fermentation liquid cannot reach the expected value; above the optimal proportion, the yeast extract powder is heavy in salty and fresh taste, and the taste of the fermentation liquid is influenced.
Further, the weight ratio of the auxiliary materials to the bacterial powder is 100: 1.
Further, the preparation method comprises mixing the fungus powder and then uniformly mixing with auxiliary materials;
or, the bacterial powder, the yeast extract powder and the peptone are fully mixed and then uniformly mixed with the glucose and the talcum powder.
The invention also protects the application of the direct-feed drinking water probiotic in direct drinking of livestock.
Further, the direct-feed drinking water probiotic is directly put into clear water according to the amount of 4-10 wt% (for example, 4 kg-10 kg of the direct-feed drinking water probiotic is weighed and added into 90 kg-96 kg of the clear water to prepare a 100kg liquid system), and the mixture is subjected to standing fermentation at 30-40 ℃ for 12-36h, preferably 24h, and then directly used for drinking of livestock and poultry. The temperature of 30-40 ℃ is the proper culture temperature of the bacterial powder. When the content is lower than 4%, the input amount of the original strains is insufficient, and the risk of mixed bacteria pollution is increased; above 10%, the nutrient content is too high, and the osmotic pressure of the fermentation liquor is too high, which affects the growth of the added strains.
The standing fermentation can be carried out in a plastic or stainless steel barrel or tank, and the barrel or tank is required to be in an environment of 30-40 ℃ or is provided with a heating temperature control device.
Compared with the prior art, the invention has the main advantages that:
(1) the conventional methods include: mixing the probiotic powder and the feed raw materials, processing and feeding, inoculating the probiotic into the feed raw materials, fermenting and feeding. The two modes have the problems that a high-temperature stage exists in the feed processing process, most of probiotics, particularly lactobacillus, cannot resist high temperature, and the content of the probiotics in the processed feed is extremely low. The invention solves the problem of probiotic loss in feed processing, and greatly improves the intake of probiotics for livestock and poultry.
(2) The operation in a conventional mode is carried out in a feed factory, the invention has wide application range, can be implemented in the feed factory or a breeding site, has low requirement on the site and small occupied area;
(3) compared with the conventional mode, the method is simple, convenient and quick to operate, low in investment cost and low in requirement on professional skills of operators, and is suitable for most farmers and small scattered households.
The invention is suitable for microecologics and animal husbandry.
Detailed Description
The following detailed description of embodiments of the invention is intended to be illustrative of the invention and is not to be construed as limiting the invention. The examples do not specify particular techniques or conditions, and are performed according to the techniques or conditions described in the literature in the art or according to the product specifications. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products commercially available.
The direct-feed drinking water probiotic preparation is characterized by comprising 0.2-0.5 part by weight of lactobacillus casei, 0.2-0.5 part by weight of lactobacillus plantarum and 0.1-0.3 part by weight of yeast, and auxiliary materials of 30-60 parts by weight of glucose, 10-20 parts by weight of yeast extract powder, 10-20 parts by weight of peptone and 20-30 parts by weight of talcum powder.
Further, the composition comprises the following components: 0.4 part by weight of lactobacillus casei, 0.4 part by weight of lactobacillus plantarum, 0.2 part by weight of yeast, 50 parts by weight of glucose, 12.5 parts by weight of yeast extract powder, 12.5 parts by weight of peptone and 25 parts by weight of talcum powder.
Further, the yeast accounts for 20% of the total flora weight; the weight ratio of the lactobacillus casei to the lactobacillus plantarum is 1: 1.
Furthermore, in the auxiliary materials, the talcum powder accounts for 1/4 of the total weight; the weight ratio of glucose to peptone is 4: 1; 1/8 yeast extract powder is used as main material; more preferably, the weight ratio of the glucose to the yeast extract powder to the peptone to the talc is 2:0.5:0.5: 1.
Further, the weight ratio of the auxiliary materials to the bacterial powder is 100: 1.
Further, the preparation method comprises mixing the fungus powder and then uniformly mixing with auxiliary materials;
or the like, or, alternatively,
and after the bacterial powder is fully mixed with the yeast extract powder and the peptone, the bacterial powder is uniformly mixed with the glucose and the talcum powder.
The direct-feeding drinking water probiotic is used for livestock to directly drink.
Further, the direct-feed drinking water probiotic agent is directly added into clear water according to the amount of 4-10 wt%, and is subjected to standing fermentation at the temperature of 30-40 ℃ for 12-36h, preferably 24h, and then directly used for livestock and poultry to drink.
Example 1: preparing the drinking water probiotics for direct-feed:
weighing 2kg of lactobacillus casei powder, 2kg of lactobacillus plantarum powder and 1kg of yeast powder, and uniformly mixing in a three-dimensional mixer with the capacity of 15kg to obtain 5kg of mixed bacteria powder.
And uniformly mixing the 5kg of mixed bacteria powder, 62.5kg of yeast extract powder and 62.5kg of peptone in a three-position three-dimensional mixer with the capacity of 200kg, and then uniformly mixing the mixed bacteria powder, 250kg of glucose and 125kg of talcum powder in a double-shaft paddle mixer with the capacity of 500kg to obtain the finished product of the direct-feed drinking water probiotic.
Example 2: preparing the drinking water probiotics for direct-feed:
weighing 10kg of lactobacillus casei powder, 10kg of lactobacillus plantarum powder and 5kg of yeast powder, and uniformly mixing in a 100kg three-dimensional mixer to obtain 25kg of mixed bacteria powder.
And uniformly mixing the 25kg of mixed bacteria powder, 312.5kg of yeast extract powder and 312.5kg of peptone in a 1000kg three-position three-dimensional mixer, and then uniformly mixing the mixed bacteria powder, 1250kg of glucose and 625kg of talcum powder in a 2500kg double-shaft paddle mixer to obtain the finished product of the direct-feed drinking water probiotic.
Example 3: preparing the drinking water probiotics for direct-feed:
0.2kg of lactobacillus casei powder, 0.2kg of lactobacillus plantarum powder and 0.1kg of yeast powder are weighed and uniformly mixed in a 1kg three-dimensional mixer to obtain 0.5kg of mixed bacteria powder.
And uniformly mixing 0.5kg of mixed bacteria powder, 6.25kg of yeast extract powder and 6.25kg of peptone in a 20kg three-position three-dimensional mixer, and uniformly mixing the mixed bacteria powder, 25kg of glucose and 12.5kg of talcum powder in a 50kg double-shaft paddle mixer to obtain the finished product of the direct-feed drinking water probiotic.
Example 4: the direct-feed drinking water probiotics are used:
weighing 5kg of the finished direct-feed drinking water probiotic product obtained in the embodiments 1 to 3, putting the weighed finished product into a 150L stainless steel tank, adding 95kg of tap water, putting into a heating rod, controlling the water temperature to be 30-40 ℃, and standing and culturing for 24h to obtain the bacterial liquid which can be directly fed. The pH of the obtained bacterial liquid is reduced to 3.8-4.2, wherein the total number of viable bacteria of the lactobacillus casei and the lactobacillus plantarum reaches 2 multiplied by 108cfu/ml～4×108cfu/ml, yeast reaches ten million levels per milliliter, salmonella, escherichia coli and staphylococcus aureus are not detected, fluoride is less than or equal to 2.0mg/L, cyanide is less than or equal to 0.05mg/L, arsenic is less than or equal to 0.20mg/L, cadmium is less than or equal to 0.01mg/L, chromium is less than or equal to 0.05mg/L, lead is less than or equal to 0.10mg/L, and mercury is less than or equal to 0.001 mg/L.
The feeding effect of the pigs is as follows:
the 100L of the bacterial liquid obtained above is injected into a water storage tank of a 1000-head pig farm for feeding in one day. When the pig is fed for a feeding period (60 days is a feeding period), compared with 1000 pigs which normally drink tap water, the diarrhea rate is reduced by 60 percent, the daily gain is increased by 16 percent, the feed-meat ratio is reduced by 0.04, and the breeding effect is obviously improved.
Example 5: the direct-feed drinking water probiotics are used:
weighing 4kg of the finished direct-feed drinking water probiotic preparation obtained in the embodiments 1 to 3, putting the weighed finished direct-feed drinking water probiotic preparation into a 150L stainless steel tank, adding 96kg of tap water, putting a heating rod, controlling the water temperature to be 30-40 ℃, and standing and culturing for 12h to obtain the bacterial liquid for direct feeding. The pH of the obtained bacterial liquid is reduced to 3.8-4.2, wherein the total number of viable bacteria of the lactobacillus casei and the lactobacillus plantarum reaches 2 multiplied by 108cfu/ml～4×108cfu/ml, yeast reaches ten million levels per milliliter, salmonella, escherichia coli and staphylococcus aureus are not detected, fluoride is less than or equal to 2.0mg/L, cyanide is less than or equal to 0.05mg/L, arsenic is less than or equal to 0.20mg/L, cadmium is less than or equal to 0.01mg/L, chromium is less than or equal to 0.05mg/L, lead is less than or equal to 0.10mg/L, and mercury is less than or equal to 0.001 mg/L.
The feeding effect of the broiler chickens is as follows:
the 100L of the bacterial liquid obtained above is injected into a water storage tank of a 10000 feather broiler farm for feeding in one day. When the broiler chickens are fed for a feeding period (45 days is one feeding period), compared with 10000 feather broilers which normally drink tap water, the survival rate is improved by 4%, the slaughter weight is increased by 2.2%, the feed-meat ratio is reduced by 4.7%, and the breeding effect is obviously improved.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made in the above embodiments by those of ordinary skill in the art without departing from the principle and spirit of the present invention.

Claims (8)

1. The direct-feed drinking water probiotic preparation is characterized by comprising 0.2-0.5 part by weight of lactobacillus casei, 0.2-0.5 part by weight of lactobacillus plantarum and 0.1-0.3 part by weight of yeast, and auxiliary materials of 30-60 parts by weight of glucose, 10-20 parts by weight of yeast extract powder, 10-20 parts by weight of peptone and 20-30 parts by weight of talcum powder.
2. The direct feed drinking water probiotic as claimed in claim 1, characterized by comprising the following ingredients: 0.4 part by weight of lactobacillus casei, 0.4 part by weight of lactobacillus plantarum, 0.2 part by weight of yeast, 50 parts by weight of glucose, 12.5 parts by weight of yeast extract powder, 12.5 parts by weight of peptone and 25 parts by weight of talcum powder.
3. The direct feed drinking water probiotic of claim 1 wherein the yeast represents 20% of the total population by weight; the weight ratio of the lactobacillus casei to the lactobacillus plantarum is 1: 1.
4. The direct-feed drinking water probiotic preparation according to claim 1, characterized in that the talc accounts for 1/4 of the total weight of the adjuvant; the weight ratio of glucose to peptone is 4: 1; 1/8 yeast extract powder is used as main material; more preferably, the weight ratio of the glucose to the yeast extract powder to the peptone to the talc is 2:0.5:0.5: 1.
5. The direct-feed drinking water probiotic as claimed in claim 1, characterized in that the weight ratio of the adjuvant to the bacterial powder is 100: 1.
6. The direct-feed drinking water probiotic preparation according to claim 1, which is prepared by mixing the bacterial powder and then uniformly mixing with the auxiliary materials;
or the like, or, alternatively,
and after the bacterial powder is fully mixed with the yeast extract powder and the peptone, the bacterial powder is uniformly mixed with the glucose and the talcum powder.
7. Use of the direct feed drinking water probiotic of claim 1 for direct consumption by livestock.
8. The use of the direct-feed drinking water probiotic as claimed in claim 1 for direct drinking by livestock, wherein the direct-feed drinking water probiotic is directly added into clear water in an amount of 4-10 wt%, and is allowed to stand and ferment at 30-40 ℃ for 12-36h, preferably 24h, before being directly used for drinking by livestock and poultry.
CN201910698001.0A 2019-07-31 2019-07-31 Direct-feed drinking water probiotic and application thereof Pending CN111184129A (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104957360A (en) * 2014-02-27 2015-10-07 巴罗顿-S.F.株式会社 Nonspecific immunostimulant composition, method of preparation the same, and its use
CN106962609A (en) * 2017-04-01 2017-07-21 福建龙岩闽雄生物科技股份有限公司 A kind of direct putting type biological fermentation feed additive and its production and use

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104957360A (en) * 2014-02-27 2015-10-07 巴罗顿-S.F.株式会社 Nonspecific immunostimulant composition, method of preparation the same, and its use
CN106962609A (en) * 2017-04-01 2017-07-21 福建龙岩闽雄生物科技股份有限公司 A kind of direct putting type biological fermentation feed additive and its production and use

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