CN111172011A

CN111172011A - Bioreactor

Info

Publication number: CN111172011A
Application number: CN202010029915.0A
Authority: CN
Inventors: 杨冬生
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-01-13
Filing date: 2020-01-13
Publication date: 2020-05-19

Abstract

The invention discloses a bioreactor, which structurally comprises a bottom frame, a reaction tank, a heat exchange plate, a tank cover, a driving box, a feed inlet and an air increasing and mixing structure, wherein the reaction tank is vertically arranged at the upper end of the bottom frame, the heat exchange plate is embedded and arranged at the inner side of the reaction tank, the tank cover is horizontally arranged at the upper end of the reaction tank, the driving box penetrates through the upper end of the tank cover, the feed inlet is arranged at the outer side of the tank cover, the air increasing and mixing structure comprises a main shaft, an air inlet structure, a communicating pipe, a rotating rod and a mixing structure, the main shaft is vertically arranged at the upper end of the air inlet structure, the communicating pipe is arranged at the lower end of the air inlet structure, the rotating rod is mechanically connected with the lower end of the communicating pipe, and the mixing; the lower end of the connecting pipe is provided with the gas mixing structure, so that oxygen in the culture solution is distributed more widely, biological reaction is better carried out, and the reaction yield is improved.

Description

Bioreactor

Technical Field

The invention belongs to the field of biological reaction, and particularly relates to a bioreactor.

Background

Since a biological reaction is a reaction that takes advantage of biological functions of an organism itself to produce a desired target product and culture the product by simulating the growth environment of the organism in a container, it is necessary to use a container such as a bioreactor to react the organism, and in order to increase the yield of the target product, it is necessary to put a large amount of the organism therein to perform the reaction, but the prior art has the following disadvantages:

because the density of the organisms reacting in the container is high and the organisms grow more densely, more oxygen is needed, and the oxygen is slowly diffused when the organisms are directly introduced into the culture solution or the container, so that the oxygen obtained by the organisms is uneven, partial organisms are dead due to oxygen deficiency, the reaction effect of the organisms is influenced, and the reaction yield is reduced.

The present application thus proposes a bioreactor which is improved with respect to the above-mentioned drawbacks.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide a bioreactor to solve the problems that in the prior art, as the density of organisms reacting in a container is high and the organisms grow more densely, more oxygen is needed and is directly introduced into a culture solution or the container, so that the oxygen diffusion is slow, the oxygen quantity obtained by the organisms is uneven, partial organisms are dead due to oxygen deficiency, the biological reaction effect is influenced, and the reaction yield is reduced.

In order to achieve the purpose, the invention is realized by the following technical scheme: a bioreactor structurally comprises a bottom frame, a reaction tank, a heat exchange plate, a tank cover, a driving box, a feeding hole and a gas increasing and mixing structure, wherein the reaction tank is vertically arranged at the upper end of the bottom frame and welded with the bottom frame; the air increasing and mixing structure comprises a main shaft, an air inlet structure, a communicating pipe, a rotating rod and a mixing structure, wherein the main shaft is vertically arranged at the upper end of the air inlet structure and is connected with the air inlet structure through a hinge, the communicating pipe is arranged at the lower end of the air inlet structure and is communicated with the inner side of the air inlet structure, the rotating rod is mechanically connected to the lower end of the communicating pipe, and the mixing structure is arranged at the lower end of the.

The invention is further improved, the air inlet structure comprises a fixed seat, an air inlet pipe, a bearing and a sleeve, the sleeve is vertically arranged at the upper end of the fixed seat and is positioned on the same axis, the air inlet pipe is embedded in the inner side of the sleeve, the bearing is arranged on the outer side of the main shaft, the bearing is clamped on the inner side of the sleeve, a communication port penetrates through the inner side of the main shaft, and the communication port is communicated with the inner side of the air inlet pipe.

The invention is further improved, the rotating rod is of a hollow cylindrical structure, and the upper end of the rotating rod is provided with an air outlet and is of an integrated structure.

The invention is further improved, the mixing structure comprises a connecting seat, a stirring disc, a connecting pipe and an air mixing structure, the connecting seat is horizontally arranged at the upper end of the stirring disc and is positioned on the same axis, and the stirring disc is connected with the air mixing structure through the connecting pipe and is communicated with the inner side of the air mixing structure.

The stirring plate is further improved by comprising an inner pipe, an inner groove, stirring plates, a connecting plate and slotted holes, wherein the inner pipe is embedded and installed on the inner side of the stirring plates and is positioned on the same axis, the inner groove is formed between the connecting plates, the stirring plates are provided with a plurality of stirring plates and are uniformly distributed on the inner side of the connecting plate, and the slotted holes penetrate through the inner side of the inner pipe and are communicated with the inner groove.

The invention is further improved, the gas mixing structure comprises a connecting sleeve, a communicating structure and a gas mixing plate, the connecting sleeve is horizontally arranged at the upper end of the communicating structure and is positioned on the same axis, and the gas mixing plate is arranged outside the communicating structure and is communicated with the inside of the communicating structure.

The invention is further improved, the communication structure comprises a machine body, a through hole, an upper communication port and an inner cavity, the through hole penetrates through the inner side of the machine body, the upper communication port is arranged at the upper end of the machine body and is of an integrated structure, and the inner cavity is positioned on the inner side of the machine body and is respectively communicated with the through hole and the upper communication port.

The invention is further improved, the gas mixing plate comprises a plate body, a gas mixing opening, a rim plate and an embedded groove, the gas mixing opening penetrates through the inner side of the plate body and is communicated with the embedded groove, the rim plate is arranged on the outer side of the plate body and is of an integrated structure, and the embedded groove is embedded in the inner side of the plate body.

According to the technical scheme, the bioreactor has the following beneficial effects:

the invention sets a gas-increasing mixing structure at the lower end of the driving box, part of the oxygen enters the upper part of the inner side of the reaction tank from the gas outlet, the rest of the oxygen is sent to the mixing structure and is sent to the stirring disc by the connecting seat, at the moment, the rotating bar connected with the communicating pipe is driven by the rotation of the main shaft, the connecting disc rotates, the stirring plate at the inner side of the connecting disc mixes and stirs the culture solution, part of the oxygen is sent to the inner tank from the slotted hole at the outer side of the inner pipe and is mixed into the culture solution, the rest of the oxygen is also sent to the connecting sleeve continuously along the inner pipe through the connecting pipe, the rotation of the connecting structure also enables the gas mixing plate at the upper end to disperse organisms at the inner side, the oxygen is sent from different positions and is mixed into the culture solution, the diffusion of the oxygen is helped, the organisms can better.

According to the invention, the gas mixing structure is arranged at the lower end of the connecting pipe, the gas mixing plate at the upper end is driven to rotate by the rotation of the communicating structure and disperse organisms on the inner side, and gas introduced by the connecting sleeve enters the inner cavity along the upper communicating opening and is sent into the embedded groove from the through opening, so that oxygen is sent out from the gas mixing opening at the upper end while the organisms are dispersed by the plate body, the oxygen is more uniformly mixed into the culture solution, the oxygen distribution in the culture solution is wider, the biological reaction is better carried out, and the reaction yield is improved.

Drawings

Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:

FIG. 1 is a schematic view of a bioreactor according to the present invention;

FIG. 2 is a schematic structural diagram of the air-increasing mixing structure of the present invention;

FIG. 3 is a front view of the air intake structure of the present invention;

FIG. 4 is a schematic top view of the air inlet structure of the present invention;

FIG. 5 is a schematic view of the rotating lever of the present invention;

FIG. 6 is a schematic structural view of a hybrid structure of the present invention;

FIG. 7 is a front view of the agitator plate of the present invention;

FIG. 8 is a schematic top view of the agitator disk of the present invention;

FIG. 9 is a schematic front view of the gas mixing structure of the present invention;

FIG. 10 is a schematic top view of the gas mixing structure of the present invention;

FIG. 11 is a schematic structural view of a communication structure according to the present invention;

FIG. 12 is a schematic structural view of the air mixing plate of the present invention.

In the figure: underframe-1, reaction tank-2, heat exchange plate-3, tank cover-4, driving box-5, feed inlet-6, air-increasing mixing structure-7, main shaft-71, air inlet structure-72, communicating pipe-73, rotating rod-74, mixing structure-75, fixed seat-721, air inlet pipe-722, bearing-723, sleeve-724, communicating port-710, air outlet-740, connecting seat-751, stirring disc-752, connecting pipe-753, air mixing structure-754, inner pipe-52 a, inner groove-52 b, stirring plate-52 c, connecting disc-52 d, slotted hole-52 e, connecting sleeve-54 a, communicating structure-54 b, air mixing plate-54 c, machine body-b 1, through port-b 2, opening-54, An upper communicating port-b 3, an inner cavity-b 4, a plate body-c 1, an air mixing port-c 2, a border plate-c 3 and an embedded groove-c 4.

Detailed Description

In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.

The first embodiment is as follows: referring to fig. 1-10, the embodiments of the present invention are as follows:

the structure of the device comprises a base frame 1, a reaction tank 2, a heat exchange plate 3, a tank cover 4, a driving box 5, a feeding port 6 and an air-increasing mixing structure 7, wherein the reaction tank 2 is vertically arranged at the upper end of the base frame 1 and welded with the same, the heat exchange plate 3 is embedded and arranged at the inner side of the reaction tank 2, the tank cover 4 is horizontally arranged at the upper end of the reaction tank 2 and is mechanically connected, the driving box 5 penetrates through the upper end of the tank cover 4 and is connected with the upper end of the air-increasing mixing structure 7, and the feeding port 6 is arranged at the outer side of the; gas increasing mixed structure 7 includes main shaft 71, air inlet structure 72, communicating pipe 73, dwang 74, mixed structure 75, main shaft 71 is vertical to be installed in air inlet structure 72 upper end and to adopt hinged joint, communicating pipe 73 is installed in air inlet structure 72 lower extreme and inboard to be linked together, dwang 74 mechanical connection is in communicating pipe 73 lower extreme, mixed structure 75 is installed in dwang 74 lower extreme and looks joint.

Referring to fig. 3-4, the air intake structure 72 includes a fixed seat 721, an air intake pipe 722, a bearing 723, and a sleeve 724, the sleeve 724 is vertically installed on the upper end of the fixed seat 721 and located on the same axis, the air intake pipe 722 is embedded inside the sleeve 724, the bearing 723 is installed on the outer side of the main shaft 71, the bearing 723 is clamped inside the sleeve 724, a communication port 710 penetrates through the inner side of the main shaft 71, the communication port 710 is communicated with the inner side of the air intake pipe 722, and when the main shaft 71 rotates, the air is kept to be introduced, and the air is better provided for the inner side.

Referring to fig. 5, the rotating rod 74 is a hollow cylindrical structure, the upper end of the rotating rod 74 is provided with an air outlet 740 and is an integrated structure, and a part of air at a flow separation position can be naturally dissolved into the culture solution at the same time of conveying the air.

Referring to fig. 6, the mixing structure 75 includes a connecting base 751, a stirring plate 752, a connecting pipe 753, and a gas mixing structure 754, wherein the connecting base 751 is horizontally installed on the upper end of the stirring plate 752 and is located on the same axis, the stirring plate 752 is connected with the gas mixing structure 754 through the connecting pipe 753, and the inner side of the stirring plate is communicated with the gas mixing structure 754, so that gas can be mixed into the culture solution while stirring, and oxygen can be conveniently supplied to the organism.

Referring to fig. 7 to 8, the stirring plate 752 includes an inner tube 52a, an inner groove 52b, a stirring plate 52c, a connecting plate 52d, and a plurality of slots 52e, wherein the inner tube 52a is inserted into and mounted on the same axis inside the stirring plate 52c, the inner groove 52b is disposed between the connecting plate 52d, the stirring plate 52c is disposed inside the connecting plate 52d, and the slots 52e penetrate inside the inner tube 52a and communicate with the inner groove 52b, so as to transport gas while stirring, and better mix with the culture solution.

Referring to fig. 9-10, the gas mixing structure 754 includes a connection sleeve 54a, a communication structure 54b, and a gas mixing plate 54c, the connection sleeve 54a is horizontally installed on the upper end of the communication structure 54b and is located on the same axis, and the gas mixing plate 54c is installed outside and inside the communication structure 54b and is communicated with each other, so as to perform biological dispersion and mix gas.

Based on the above embodiment, the specific working principle is as follows:

the culture solution and organisms are fed into the inner side of the reaction tank 2 by the feed inlet 6, the oxygen pipeline is connected with the air inlet pipe 722, the operation can be started, the driving box 5 is opened to operate and drive the mixing structure 7, at the moment, the main shaft 71 rotates and rotates along the bearing 723 on the inner side of the sleeve 724, at the moment, the oxygen introduced into the air inlet pipe 722 enters from the communication port 710 and enters into the inner side of the rotating rod 74 at the lower end along the fixed seat 721, part of the oxygen firstly enters into the upper part of the inner side of the reaction tank 2 from the air outlet 740, the rest of the oxygen is then fed into the mixing structure 75 and is fed to the stirring disk 752 by the connecting seat 751, at the moment, the rotating rod 74 connected with the communication pipe 73 is driven by the rotation of the main shaft 71, the connecting disk 52d rotates, the stirring plate 52c on the inner side mixes and stirs the culture solution, and part of the oxygen is, the rest of the oxygen will continue to flow through the inner tube 52a to the connection sleeve 54a through the connection tube 753, and the rotation of the connection structure 54b will also disperse the living organisms inside the upper gas mixing plate 54 c.

Example two: referring to fig. 9-12, the embodiment of the present invention is as follows:

the air mixing structure 754 comprises a connecting sleeve 54a, a communicating structure 54b and an air mixing plate 54c, wherein the connecting sleeve 54a is horizontally arranged at the upper end of the communicating structure 54b and is positioned on the same axis, and the air mixing plate 54c is arranged outside the communicating structure 54b and is communicated with the inside.

Referring to fig. 11, the communicating structure 54b includes a body b1, a through hole b2, an upper communicating port b3, and an inner cavity b4, the through hole b2 penetrates the inside of the body b1, the upper communicating port b3 is disposed at the upper end of the body b1 and is an integrated structure, the inner cavity b4 is located inside the body b1 and is communicated with the through hole b2 and the upper communicating port b3, and gas can be introduced into the inner cavity b4 and dispersed through the through hole b 2.

Referring to fig. 12, the gas mixing plate 54c includes a plate body c1, a gas mixing opening c2, an edge plate c3 and an embedded groove c4, the gas mixing opening c2 penetrates through the inner side of the plate body c1 and is communicated with the embedded groove c4, the edge plate c3 is installed on the outer side of the plate body c1 and is of an integrated structure, the embedded groove c4 is embedded in the inner side of the plate body c1, gas is dispersed from the gas mixing opening c2 of the plate body c1, and is mixed into the culture solution more uniformly, so as to supply oxygen to the organism.

Based on the above embodiment, the specific working principle is as follows:

first, the communicating structure 54b rotates, the gas mixing plate 54c at the upper end is driven by the communicating structure to rotate and disperse the organisms at the inner side, and the gas introduced by the connecting sleeve 54a enters the inner cavity b4 along the upper communicating opening b3 and is sent into the embedded groove c4 from the opening b2, at the moment, the plate c1 disperses the organisms, and the gas mixing opening c2 at the upper end also sends oxygen, so that the oxygen is more uniformly mixed into the culture solution.

The invention solves the problems that in the prior art, because the density of organisms reacting in a container is high and the organisms grow densely, more oxygen is needed and is directly introduced into a culture solution or the container, the oxygen diffusion is slow, so that the oxygen obtained by the organisms is uneven, partial organisms are anoxic and die, the reaction effect of the organisms is further influenced, and the reaction yield is reduced, through the mutual combination of the components, the lower end of a driving box is provided with an air-increasing mixing structure, partial oxygen firstly enters the upper part of the inner side of a reaction tank from an air outlet, the rest oxygen is then fed into the mixing structure and is fed to a stirring disc by a connecting seat, at the moment, a rotating rod connected with a communicating pipe is driven by the rotation of a main shaft, the connecting disc rotates, so that the stirring plate at the inner side of the connecting disc mixes and stirs the culture solution, and partial oxygen is also fed into an inner tank from a slotted hole at the outer side of an inner pipe and, the rest oxygen is continuously sent to the connecting sleeve through the connecting pipe along the inner pipe, and the rotation of the communicating structure also enables the gas mixing plate at the upper end to disperse the organisms at the inner side, the oxygen is sent from different positions and mixed into the culture solution to help the oxygen to diffuse, so that the organisms can better obtain the oxygen, and the death of the organisms due to oxygen deficiency is reduced; set up at the connecting pipe lower extreme and mixed the gas structure, the rotation of connectivity structure will drive the gas board that mixes of upper end and rotate and disperse the organism of inboard, and the gas that utilizes the adapter sleeve to let in will be in the same direction as last intercommunication mouth entering inner chamber and send into the embedded groove from the opening, the plate body is when carrying out the organism dispersion this moment, the gas port that mixes of upper end also will send out oxygen, with oxygen in the more even sneaking into culture solution, oxygen distribution is wider in making the culture solution, better carry out biological reaction, the reaction output is improved.

While there have been shown and described what are at present considered the fundamental principles and essential features of the invention and its advantages, it will be apparent to those skilled in the art that the invention is not limited to the details of the foregoing exemplary embodiments, but is capable of other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. A bioreactor structurally comprises a base frame (1), a reaction tank (2), a heat exchange plate (3), a tank cover (4), a driving box (5), a feeding hole (6) and a gas increasing mixing structure (7), wherein the reaction tank (2) is vertically arranged at the upper end of the base frame (1), the heat exchange plate (3) is embedded into the reaction tank (2), the tank cover (4) is horizontally arranged at the upper end of the reaction tank (2), the driving box (5) penetrates through the upper end of the tank cover (4) and is connected with the upper end of the gas increasing mixing structure (7), and the feeding hole (6) is arranged at the outer side of the tank cover (4); the method is characterized in that:

gas increase mixed structure (7) include main shaft (71), inlet structure (72), communicating pipe (73), dwang (74), mixed structure (75), main shaft (71) is vertical to be installed in inlet structure (72) upper end, install in inlet structure (72) lower extreme communicating pipe (73), dwang (74) mechanical connection is in communicating pipe (73) lower extreme, mixed structure (75) are installed in dwang (74) lower extreme.

2. A bioreactor according to claim 1, wherein: the air inlet structure (72) comprises a fixed seat (721), an air inlet pipe (722), a bearing (723) and a sleeve (724), wherein the sleeve (724) is vertically arranged at the upper end of the fixed seat (721), the air inlet pipe (722) is embedded in the inner side of the sleeve (724), the bearing (723) is arranged on the outer side of the main shaft (71), the bearing (723) is clamped on the inner side of the sleeve (724), a communicating port (710) penetrates through the inner side of the main shaft (71), and the communicating port (710) is communicated with the inner side of the air inlet pipe (722).

3. A bioreactor according to claim 1, wherein: dwang (74) are the cylinder structure of fretwork, dwang (74) upper end is equipped with gas outlet (740).

4. A bioreactor according to claim 1, wherein: the mixing structure (75) comprises a connecting seat (751), a stirring disc (752), a connecting pipe (753) and an air mixing structure (754), wherein the connecting seat (751) is horizontally arranged at the upper end of the stirring disc (752), and the stirring disc (752) is connected with the air mixing structure (754) through the connecting pipe (753).

5. A bioreactor according to claim 4, wherein: the stirring plate (752) comprises an inner pipe (52a), an inner groove (52b), stirring plates (52c), a connecting plate (52d) and a slotted hole (52e), the inner pipe (52a) is embedded in the inner side of the stirring plates (52c), the inner groove (52b) is formed between the connecting plates (52d), the stirring plates (52c) are arranged in a plurality and are uniformly distributed on the inner side of the connecting plate (52d), and the slotted hole (52e) penetrates through the inner side of the inner pipe (52 a).

6. A bioreactor according to claim 4, wherein: the gas mixing structure (754) comprises a connecting sleeve (54a), a communicating structure (54b) and a gas mixing plate (54c), wherein the connecting sleeve (54a) is horizontally arranged at the upper end of the communicating structure (54b), and the gas mixing plate (54c) is arranged at the outer side of the communicating structure (54b) and communicated with the inner side.

7. A bioreactor according to claim 6, wherein: the communicating structure (54b) comprises a machine body (b1), a through opening (b2), an upper communicating opening (b3) and an inner cavity (b4), wherein the through opening (b2) penetrates through the inner side of the machine body (b1), the upper communicating opening (b3) is arranged at the upper end of the machine body (b1), and the inner cavity (b4) is located on the inner side of the machine body (b 1).

8. A bioreactor according to claim 6, wherein: mix gas board (54c) including plate body (c1), mix gas mouth (c2), along board (c3), embedded groove (c4), mix gas mouth (c2) and run through in plate body (c1) inboard, install in plate body (c1) outside along board (c3), embedded groove (c4) are embedded in plate body (c1) inboard.