CN111166714B - Enzyme bath composition and preparation method and application thereof - Google Patents
Enzyme bath composition and preparation method and application thereof Download PDFInfo
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- CN111166714B CN111166714B CN202010062652.3A CN202010062652A CN111166714B CN 111166714 B CN111166714 B CN 111166714B CN 202010062652 A CN202010062652 A CN 202010062652A CN 111166714 B CN111166714 B CN 111166714B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9755—Gymnosperms [Coniferophyta]
- A61K8/9761—Cupressaceae [Cypress family], e.g. juniper or cypress
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9755—Gymnosperms [Coniferophyta]
- A61K8/9767—Pinaceae [Pine family], e.g. pine or cedar
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/10—Washing or bathing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q7/00—Preparations for affecting hair growth
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Abstract
The invention provides an enzyme bath composition and a preparation method and application thereof, and relates to the technical field of enzyme baths. The ferment bath composition is prepared by raw materials such as base material, rice bran, corn, saccharomycetes, bacillus subtilis, odor removing bacteria, water and the like, wherein, the saccharomycetes and the bacillus subtilis are mainly used as zymogens to ferment the base material, the odor removing bacteria are mainly used for degrading peculiar smell possibly generated by the saccharomycetes and the bacillus subtilis, the rice bran and the corn mainly provide a large amount of nutrient substances for the saccharomycetes, the bacillus subtilis and the odor removing bacteria to ensure the smooth operation of the fermentation process, through the matching of the raw materials, the ferment bath composition has higher organic matter and nitrogen content, the organic matter in the ferment bath composition can be fully decomposed into beneficial micromolecules such as ferment and the like which are easier to be absorbed by human bodies by fungi such as saccharomycetes, bacillus subtilis, odor removing bacteria and the like, thereby promote the health care efficiency of ferment bath composition, this ferment bath composition taste is fragrant simultaneously, has guaranteed user's good experience.
Description
Technical Field
The invention belongs to the technical field of enzyme baths, and particularly relates to an enzyme bath composition and a preparation method and application thereof.
Background
The ferment bath is a unique ferment health preserving warm bath, which originally originated in japan. At present, the health preserving industry in China is greatly developed, and the enzyme bath is popular in China.
However, the organic matter content and nitrogen content of the enzyme bath composition adopted in the current enzyme bath are low, so that the produced enzymes and other beneficial small molecules are few, and the health care effect on human bodies is limited. In addition, sometimes the ferment bath composition may generate unpleasant smell during the use process, so that the experience and the feeling of the user are poor.
In view of the above, the present invention is particularly proposed to solve at least one of the above technical problems.
Disclosure of Invention
The first objective of the present invention is to provide a ferment bath composition, which has high organic matter and nitrogen content, wherein the organic matter can be sufficiently decomposed into beneficial small molecules such as ferment by fungi such as yeast, bacillus subtilis, and odor removing bacteria, and the ferment bath composition has a fragrant taste, thereby ensuring good experience and feeling of users.
The second objective of the present invention is to provide a method for preparing the enzyme bath composition.
The third objective of the present invention is to provide an application of the enzyme bath composition.
In order to achieve the purpose, the invention adopts the technical scheme that:
the invention provides a ferment bath composition which comprises the following raw materials in parts by weight:
3000-3500 parts of base material, 1500-2000 parts of rice bran, 200-250 parts of corn, 1.0-1.5 parts of yeast, 1.5-2.0 parts of bacillus subtilis, 0.8-1.2 parts of odor removing bacteria and 500-800 parts of water.
Further, on the basis of the above technical scheme of the present invention, the enzyme bath composition comprises the following raw materials in parts by weight:
base material 3020-.
Further, on the basis of the above technical scheme of the present invention, the enzyme bath composition comprises the following raw materials in parts by weight:
3050-3450 parts of base material, 1550-1950 parts of rice bran, 245 parts of corn 210-245 parts of yeast, 1.2-1.4 parts of bacillus subtilis, 1.0-1.2 parts of odor-removing bacteria and 510-790 parts of water.
Further, on the basis of the technical scheme of the invention, the content of the yeast is 100-200 hundred million cfu/g;
preferably, the bacillus subtilis has the bacterium content of 800-1200 hundred million cfu/g;
preferably, the bacterium content of the odor removing bacterium is 80-120 hundred million cfu/g.
Further, on the basis of the technical scheme of the invention, the base material comprises pine wood chips and/or cypress wood chips;
preferably, the odour-combating bacteria comprise bacillus coagulans and/or lactic acid bacteria.
Further, on the basis of the technical scheme of the invention, the rice bran is rice bran powder, and the mesh number of the rice bran powder is 60-80 meshes;
preferably, the corn is corn flour, and the mesh number of the corn flour is 100-120 meshes.
Further, on the basis of the technical scheme, raw materials of the ferment bath composition further comprise bacillus licheniformis, wherein the weight part of the bacillus licheniformis is 0.5-1.0 part;
preferably, the bacteria content of the bacillus licheniformis is 800-1200 hundred million cfu/g.
Further, on the basis of the technical scheme, the mass content of organic matters in the enzyme bath composition is 75-85%;
preferably, the nitrogen content of the ferment bath composition is 3-7% by mass.
The invention also provides a preparation method of the enzyme bath composition, which comprises the following steps:
mixing the base material, rice bran, corn, saccharomycetes, bacillus subtilis, odor removing bacteria, water and optionally bacillus licheniformis according to the formula amount, and then fermenting to obtain the enzyme bath composition;
preferably, the temperature of the fermentation is 55-70 ℃, preferably 56-68 ℃;
preferably, the fermentation time is 24-48h, preferably 25-45 h.
The invention also provides an application of the ferment bath composition or the ferment bath composition prepared by the preparation method of the ferment bath composition in ferment bath.
Compared with the prior art, the invention has the beneficial effects that:
(1) the invention provides a ferment bath composition, which is prepared from specific raw materials such as base materials, rice bran, corn, saccharomycetes, bacillus subtilis, odor removing bacteria and water, wherein the saccharomycetes and the bacillus subtilis are mainly used as fermenting bacteria to ferment the base materials to prepare the ferment bath composition, the odor removing bacteria are mainly used for degrading peculiar smell possibly generated by the saccharomycetes and the bacillus subtilis, and the rice bran and the corn mainly provide a large amount of nutrient substances for the saccharomycetes, the bacillus subtilis and the odor removing bacteria to ensure that the fermentation process is smoothly carried out. Through the cooperation of above-mentioned each raw materials for have higher organic matter and nitrogen content in the ferment bath composition, organic matter can be by the saccharomycetes, bacillus subtilis and remove fungus such as fungus that the flavor fungus fully decomposes into beneficial micromolecule such as ferment, beneficial micromolecule such as above-mentioned ferment is more easily absorbed by the human body in the ferment bath composition use, thereby promote the health care efficiency of ferment bath composition, this ferment bath composition taste faint scent does not have the extraneous odour simultaneously, guaranteed the good experience and the degree of experience of user.
When a user uses the enzyme bath composition, a body discharges a large amount of sweat by utilizing heat generated by fermentation of the enzyme bath composition, and under the action of beneficial micromolecules such as enzyme, the blood circulation of the body is promoted through the respiration of the skin, and the endotoxin in the body is discharged out of the body, so that the effects of improving the physique, protecting and whitening the skin, enhancing the body resistance, the immunity and the like are achieved.
(2) The invention provides a preparation method of an enzyme bath composition, which is simple and convenient to operate and easy to produce, and the obtained enzyme bath composition is relatively stable in quality.
(3) The present invention provides an application of a ferment bath composition, which has good application in a ferment bath in view of the advantages of the ferment bath composition.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
Fig. 1 is a schematic diagram of a ferment bath composition provided in example 1 of the present invention.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to embodiments and examples, but those skilled in the art will understand that the following embodiments and examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. Those who do not specify the conditions are performed according to the conventional conditions or the conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
According to a first aspect of the present invention, there is provided a ferment bath composition, comprising the following raw materials in parts by weight:
3000-3500 parts of base material, 1500-2000 parts of rice bran, 200-250 parts of corn, 1.0-1.5 parts of yeast, 1.5-2.0 parts of bacillus subtilis, 0.8-1.2 parts of odor removing bacteria and 500-800 parts of water.
The invention provides a ferment bath composition, which is prepared from specific raw materials such as base materials, rice bran, corn, saccharomycetes, bacillus subtilis, odor removing bacteria and water, wherein the saccharomycetes and the bacillus subtilis are mainly used as fermenting bacteria to ferment the base materials to prepare the ferment bath composition, the odor removing bacteria are mainly used for degrading peculiar smell possibly generated by the saccharomycetes and the bacillus subtilis, and the rice bran and the corn mainly provide a large amount of nutrient substances for the saccharomycetes, the bacillus subtilis and the odor removing bacteria to ensure that the fermentation process is smoothly carried out. Through the cooperation of above-mentioned each raw materials for have higher organic matter and nitrogen content in the ferment bath composition, organic matter in the ferment bath composition can be by fungi such as saccharomycetes, bacillus subtilis and remove the flavor fungus fully to be decomposed into beneficial micromolecules such as ferment, beneficial micromolecules such as above-mentioned ferment are more easily absorbed by the human body in the ferment bath composition use, thereby promote the health care efficiency of ferment bath composition, this ferment bath composition taste is fragrant simultaneously, has guaranteed good experience and the degree of sensitivity of user.
Specifically, the base material is mainly used as a fermentation raw material of the enzyme bath composition. The base material is not particularly limited, and may be pine wood chips or cypress wood chips. Typical but non-limiting parts by weight of the base are 3000 parts, 3020 parts, 3050 parts, 3080 parts, 3100 parts, 3120 parts, 3150 parts, 3180 parts, 3200 parts, 3220 parts, 3250 parts, 3280 parts, 3300 parts, 3320 parts, 3350 parts, 3380 parts, 3400 parts, 3420 parts, 3450 parts, 3480 parts, or 3500 parts.
The rice bran has high nutritional value, and after the rice bran is used as one of raw materials to prepare the enzyme bath composition, the rice bran can generate beneficial substances such as rice bran small peptides and the like, so that the health-care effect of the enzyme bath composition is improved. Typical but non-limiting parts by weight of rice bran are 1500 parts, 1520 parts, 1550 parts, 1580 parts, 1600 parts, 1620 parts, 1650 parts, 1680 parts, 1700 parts, 1720 parts, 1750 parts, 1780 parts, 1800 parts, 1820 parts, 1850 parts, 1880 parts, 1900 parts, 1920 parts, 1950 parts, 1980 parts or 2000 parts.
Corn can provide a large amount of carbon sources and proteins, and is mainly used as a main nutrient substance of yeast, bacillus subtilis, odor removing bacteria and other strains. Typical but non-limiting parts by weight of corn are 200 parts, 205 parts, 208 parts, 210 parts, 212 parts, 215 parts, 218 parts, 220 parts, 222 parts, 225 parts, 228 parts, 230 parts, 232 parts, 235 parts, 238 parts, 240 parts, 242 parts, 245 parts, 248 parts or 250 parts.
The yeast and the bacillus subtilis are mainly used as zymophyte, so that the enzyme bath composition is prepared after the base material is fermented. The yeast is a fermentation broth at a medium-low temperature. In the initial stage of fermentation of the raw materials, the temperature of the raw material mixture is low, and the yeast generates heat and the like through processes of propagation, metabolism and the like, so that the temperature of the raw material mixture can be increased, and the germination of the bacillus subtilis and the odor removing bacteria is accelerated. Typical but non-limiting parts by weight of yeast are 1.0 part, 1.05 part, 1.08 part, 1.1 part, 1.15 part, 1.18 part, 1.2 part, 1.25 part, 1.28 part, 1.3 part, 1.35 part, 1.38 part, 1.4 part, 1.45 part, 1.48 part or 1.5 part.
The bacillus subtilis is a zymocyte at high temperature, and the bacillus subtilis is rapidly propagated and fermented to generate high temperature, so that the enzyme bath composition is obtained. Typical but non-limiting parts by weight of Bacillus subtilis are 1.5 parts, 1.55 parts, 1.58 parts, 1.6 parts, 1.65 parts, 1.68 parts, 1.7 parts, 1.75 parts, 1.78 parts, 1.8 parts, 1.85 parts, 1.88 parts, 1.9 parts, 1.95 parts, 1.98 parts or 2.0 parts.
The odor removing bacteria are odor degrading bacteria, and substances such as organic acid and the like generated by metabolism of the odor removing bacteria are mainly used for degrading the odor such as ammonia odor and the like generated in the fermentation process of each raw material, so that the odor of the enzyme bath composition is prevented from being generated. Typical but non-limiting parts by weight are 0.80 part, 0.82 part, 0.85 part, 0.88 part, 0.90 part, 0.92 part, 0.95 part, 0.98 part, 1.0 part, 1.05 part, 1.08 part, 1.1 part, 1.15 part, 1.18 part or 1.2 part.
Water is typically, but not limited to, 500 parts, 505 parts, 520 parts, 550 parts, 560 parts, 580 parts, 600 parts, 620 parts, 640 parts, 650 parts, 660 parts, 680 parts, 700 parts, 720 parts, 740 parts, 750 parts, 760 parts, 780 parts, or 800 parts by weight.
It should be noted that the term "comprising", "mainly prepared from … …" in the present invention means that it may include other materials, such as bacillus licheniformis, brown sugar, etc., in addition to the mentioned materials, which impart different properties to the enzyme bath composition. In addition, the terms "comprising," "prepared primarily from … …," as used herein, can be replaced by the terms "comprising" or "prepared from … …," as used herein. For example, a closed writing method can be replaced, and the enzyme bath composition is prepared from the following raw materials in parts by weight: 3000-3500 parts of base material, 1500-2000 parts of rice bran, 200-250 parts of corn, 1.0-1.5 parts of yeast, 1.5-2.0 parts of bacillus subtilis, 0.8-1.2 parts of odor removing bacteria and 500-800 parts of water.
There are also specific limitations on the amount of each raw material, for example, when the amount of the base material is too large, the bacteria content in the enzyme bath composition per unit is low, which results in a slow temperature rise rate of the enzyme bath composition as a whole, and when the amount of the base material is too small, the heat preservation performance of the enzyme bath composition as a whole is poor, and the temperature is lost too fast; when the amount of the rice bran is too large, the odor of the enzyme bath composition is aggravated in the fermentation process, and when the amount of the rice bran is too small, the nitrogen content of the enzyme bath composition is lower; when the using amount of the corn is too large, the air permeability of the enzyme bath composition is poor, and because the bacillus subtilis belongs to aerobic strains, the fermentation process of the bacillus subtilis is influenced by the too large using amount of the corn, and when the using amount of the corn is too small, the whole nutrient substances of the enzyme bath composition are insufficient to maintain the nutrition required by the strains; when the using amount of the yeast is too large, the oxygen reserved in the enzyme bath composition is utilized by the yeast, the heat released by the yeast is not enough to enable the temperature of the enzyme bath composition to be increased to the fermentation temperature in a short time, and further, the propagation metabolism and the like of bacillus subtilis are inhibited due to the utilization of the oxygen in the enzyme bath composition by the yeast, so that the effect of the whole enzyme bath composition is influenced, and when the using amount of the yeast is too small, the early-stage starting time of the enzyme bath is prolonged, and further, the overall temperature rising speed of the enzyme bath composition is influenced; when the using amount of the bacillus subtilis is too large, the cost of the enzyme bath composition is increased, the temperature of the enzyme bath composition is suddenly increased, and partial bacteria are dead, so that the odor of the enzyme bath is aggravated by increasing the amount of gases such as ammonia gas, hydrogen sulfide and the like in the enzyme bath composition, and when the using amount of the bacillus subtilis is too small, the transition time of the enzyme bath composition from a low-temperature stage to a high-temperature stage is prolonged, and the fermentation time is prolonged; when the using amount of the odor removing bacteria is too large, the cost of the whole enzyme bath composition is increased, and when the using amount is too small, the bacteria content of the odor removing bacteria in the enzyme bath composition is low, so that the odor is not thoroughly and timely treated, and the enzyme bath experience is influenced. Therefore, the dosage of each raw material should be controlled within a reasonable numerical range.
In order to realize better matching/synergistic effect among the raw materials, the invention further optimizes the dosage of the base material, rice bran, corn, saccharomycetes, bacillus subtilis, odor removing bacteria and water.
As an optional embodiment of the present invention, the ferment bath composition comprises the following raw materials in parts by weight:
base material 3020-.
As an optional embodiment of the present invention, the ferment bath composition comprises the following raw materials in parts by weight:
3050-3450 parts of base material, 1550-1950 parts of rice bran, 245 parts of corn 210-245 parts of yeast, 1.2-1.4 parts of bacillus subtilis, 1.0-1.2 parts of odor-removing bacteria and 510-790 parts of water.
Through the further restriction to each raw materials quantity of ferment bath composition for have better cooperation effect between each raw materials, thereby be favorable to ferment bath composition performance's further promotion.
As an optional embodiment of the invention, the content of the yeast is 100-. Typical, but non-limiting, yeast strains have a bacterial content of 100 hundred million cfu/g, 110 hundred million cfu/g, 120 hundred million cfu/g, 130 hundred million cfu/g, 140 hundred million cfu/g, 150 hundred million cfu/g, 160 hundred million cfu/g, 170 hundred million cfu/g, 180 hundred million cfu/g, 190 hundred million cfu/g, or 200 hundred million cfu/g.
As an alternative embodiment of the invention, the bacillus subtilis has a bacterial content of 800-. Typical, but non-limiting, Bacillus subtilis strains have a bacterial content of 800 hundred million cfu/g, 820 hundred million cfu/g, 840 hundred million cfu/g, 850 hundred million cfu/g, 860 hundred million cfu/g, 900 hundred million cfu/g, 920 hundred million cfu/g, 940 hundred million cfu/g, 950 hundred million cfu/g, 960 hundred million cfu/g, 980 hundred million cfu/g, 1000 hundred million cfu/g, 1020 hundred million cfu/g, 1050 hundred million cfu/g, 1080 hundred million cfu/g, 1100 hundred million cfu/g, 1120 hundred million cfu/g, 1150 hundred million cfu/g, 1180 hundred million cfu/g, or 1200 hundred million cfu/g.
As an alternative embodiment of the invention, the bacterium content of the odor removing bacteria is 80-120 hundred million cfu/g. Typical, but non-limiting, odor elimination bacteria have a bacterial content of 80, 82, 94, 95, 96, 98, 100, 102, 105, 108, 110, 112, 115, 118 or 120 hundred million cfu/g.
Through the specific limitation on the bacteria content of the saccharomycetes, the bacillus subtilis and the odor removing bacteria, the raw materials can achieve the effect of full fermentation under the action of the bacteria, so that the overall performance of the enzyme bath composition is better.
As an alternative embodiment of the invention, the base material comprises pine wood chips and/or cedar wood chips. It should be noted that "and/or" means that the base material may include only pine sawdust, only cypress sawdust, or both pine sawdust and cypress sawdust.
The pine sawdust is powder or powder of pine. The cypress sawdust is powder or powder made of cypress. After the pine sawdust and/or the cedar sawdust are fermented by the strains, the fragrance of the pine and/or the cedar is emitted, so that the taste of the enzyme bath composition is better smelled, and the experience or feeling of a user in using the enzyme bath composition is further improved.
As an alternative embodiment of the invention, the odour-combating bacteria comprise bacillus coagulans and/or lactic acid bacteria. As used herein, "and/or" means that the odor-removing bacteria may include only Bacillus coagulans, only lactic acid bacteria, and both Bacillus coagulans and lactic acid bacteria.
Organic acid generated by the bacillus coagulans and/or the lactic acid bacteria in the metabolic process can effectively inhibit peculiar smells such as ammonia smell generated in the fermentation process, and is beneficial to improving the experience and feeling of a user in the enzyme bath.
As an alternative embodiment of the invention, the rice bran is rice bran powder, and the mesh number of the rice bran powder is 60-80 meshes. Typical, but non-limiting, mesh sizes of the rice bran powder are 60 mesh, 70 mesh, or 80 mesh.
As an alternative embodiment of the invention, the corn is corn flour, and the mesh number of the corn flour is 100-120 meshes. Typical, but non-limiting, corn flour has a mesh size of 100, 110, or 120.
By limiting the specific forms of the rice bran and the corn, the nutrient substances of the rice bran and the corn are facilitated to be easily contacted with fungi (saccharomycetes, bacillus subtilis, odor removing bacteria and the like) in the enzyme bath composition, so that the rice bran and the corn quickly act to achieve an ideal effect. If the grain size of the rice bran and the corn flour is too small, the appearance is obviously affected, nutrient substances are more difficult to be utilized by fungi, and if the grain size is too large, the air permeability of the enzyme bath composition is affected, and the temperature rise of the enzyme bath composition is further affected.
As an optional embodiment of the present invention, the raw materials of the ferment bath composition further include bacillus licheniformis, wherein the bacillus licheniformis is 0.5-1.0 part by weight; typical but non-limiting parts by weight of Bacillus licheniformis are 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts or 1.0 parts.
The addition of the bacillus licheniformis is beneficial to the enzyme bath composition for a longer high-temperature period. The bacillus licheniformis is facultative anaerobe and can continuously act on the ferment bath composition under the anaerobic condition, and the bacillus licheniformis is a thermotolerant bacterium, so that the high-temperature period of the ferment bath composition is longer.
As an alternative embodiment of the invention, the bacteria content of Bacillus licheniformis is 800-1200 billion cfu/g, and the bacteria content of typical but non-limiting Bacillus licheniformis is 800-820-840-850, 860-850, 900-900, 920-980, 940-950, 960-980, 1000-1020, 1050-1050, 1080-1080, 1100, 1120, 1150-1150, 1180 or 1200-1200 billion cfu/g.
As an optional embodiment of the invention, the mass content of the organic matters in the ferment bath composition is 75-85%; typical but non-limiting organic matter content in the ferment bath composition is 75%, 76%, 78%, 80%, 82%, 84%, or 85% by mass.
Preferably, the nitrogen content of the ferment bath composition is 3-7% by mass. Typical but non-limiting nitrogen content of the enzyme bath composition is 3.0%, 3.2%, 3.5%, 3.6%, 3.8%, 4.0%, 4.2%, 4.5%, 4.6%, 4.8%, 5.0%, 5.2%, 5.5%, 5.6%, 5.8%, 6.0%, 6.2%, 6.5%, 6.8%, or 7.0% by mass.
According to the second aspect of the present invention, there is also provided a preparation method of the ferment bath composition, comprising the steps of:
mixing the base material, rice bran, corn, saccharomycetes, bacillus subtilis, odor removing bacteria, water and optionally bacillus licheniformis according to the formula ratio, and fermenting to obtain the enzyme bath composition.
It is to be noted that "optionally" as used herein means that Bacillus licheniformis bacteria can be selectively added, either with or without addition.
The preparation method is simple and convenient to operate and easy to produce, and the obtained enzyme bath composition is stable in quality.
As an alternative embodiment of the invention, the temperature of the fermentation is from 55 to 70 ℃ and preferably from 56 to 68 ℃. Typical, but not limiting, fermentation temperatures are 55 deg.C, 56 deg.C, 58 deg.C, 60 deg.C, 62 deg.C, 64 deg.C, 65 deg.C, 66 deg.C, 68 deg.C, 69 deg.C or 70 deg.C.
As an alternative embodiment of the invention, the fermentation time is from 24 to 48 hours, preferably from 25 to 45 hours. Typical, but not limiting, fermentation times are 24h, 25h, 26h, 28h, 30h, 32h, 34h, 35h, 36h, 38h, 40h, 42h, 44h, 45h, 46h or 48 h.
The temperature rise period in the early stage of the enzyme bath composition is not too long by limiting the time in the fermentation process, and the medium stage of the enzyme bath composition which can be used is longer by limiting the temperature in the fermentation process. If the temperature is too high, the base material (pine wood chips and/or cypress wood chips) in the enzyme bath composition may be carbonized, thereby reducing the service life of the enzyme bath composition.
According to a third aspect of the present invention, there is also provided a use of the ferment bath composition. In view of the advantages of the enzyme bath composition, the enzyme bath composition has good application in enzyme baths.
The method for using the enzyme bath composition (i.e., the method for performing the enzyme bath) provided by the present invention is to embed the human body into the enzyme bath composition, and the embedding time can be determined according to the body condition of different people. Generally, the embedding time is 10-15 min.
The body can discharge a large amount of sweat by utilizing heat generated by fermentation of the enzyme bath composition, and the body can promote blood circulation of the body and discharge toxin in the body out of the body through the respiration of the skin under the action of beneficial micromolecules such as enzyme, so that the effects of improving the physique, protecting the skin, whitening the skin, enhancing the body resistance, the immunity and the like can be achieved.
After the ferment bath composition is used for a period of time, the generated heat is reduced, and after the temperature is reduced, a new ferment bath composition can be continuously added into the original ferment bath composition to keep a certain heat dissipation, so that the efficacy of the ferment bath composition is ensured.
The present invention will be further described with reference to specific examples and comparative examples.
Example 1
The present embodiment provides a ferment bath composition, as shown in fig. 1, including the following raw materials:
350kg of pine sawdust, 150kg of rice bran powder, 20kg of corn flour, 0.15kg of yeast, 0.2kg of bacillus subtilis, 0.1kg of bacillus coagulans and 60kg of water;
wherein the bacteria content of the microzyme is 200 hundred million cfu/g, the bacteria content of the bacillus subtilis is 1000 hundred million cfu/g, and the bacteria content of the bacillus coagulans is 100 hundred million cfu/g.
Example 2
This example provides an enzyme bath composition, which is the same as example 1 except that the amount of rice bran used in the raw materials was changed to 200kg, and the amount of corn used was changed to 25 kg.
Example 3
This example provides an enzyme bath composition, which is the same as example 1 except that the amount of yeast in the raw material was changed to 0.10 kg.
Example 4
This example provides an enzyme bath composition, which is prepared by replacing the yeast content in the raw material with 100 hundred million cfu/g, and the raw materials and the amounts used are the same as those in example 1.
Example 5
This example provides an enzyme bath composition, which is the same as example 1 except that the amount of bacillus subtilis in the raw material was replaced with 0.15 kg.
Example 6
This example provides an enzyme bath composition, which was prepared in the same manner as in example 1, except that the bacterial content of Bacillus subtilis was changed to 800 hundred million cfu/g.
Example 7
This example provides an enzyme bath composition, which is the same as example 1 except that the amount of Bacillus coagulans in the raw materials was changed to 0.08 kg.
Example 8
This example provides an enzyme bath composition, which was prepared by replacing the bacterial content of Bacillus coagulans in the raw materials with 80 hundred million cfu/g, and the raw materials and the amounts used were the same as in example 1.
Example 9
This example provides a ferment bath composition, wherein the raw materials of the ferment bath composition further include 0.05kg of bacillus licheniformis, the bacteria content of the bacillus licheniformis is 1000 hundred million cfu/g, and the rest raw materials and the usage amount are the same as those in example 5.
Example 10
The present embodiment provides a ferment bath composition, including the following raw materials:
302kg of pine sawdust, 152kg of rice bran, 20kg of corn, 0.11kg of yeast, 0.16kg of bacillus subtilis, 0.12kg of bacillus coagulans and 60kg of water;
wherein the bacteria content of the microzyme is 200 hundred million cfu/g, the bacteria content of the bacillus subtilis is 1000 hundred million cfu/g, and the bacteria content of the bacillus coagulans is 100 hundred million cfu/g.
Example 11
The present embodiment provides a ferment bath composition, including the following raw materials:
320kg of pine sawdust, 180kg of rice bran, 22kg of corn, 0.12kg of yeast, 0.18kg of bacillus subtilis, 0.12kg of bacillus coagulans and 60kg of water.
Wherein the bacteria content of the microzyme is 200 hundred million cfu/g, the bacteria content of the bacillus subtilis is 1000 hundred million cfu/g, and the bacteria content of the bacillus coagulans is 100 hundred million cfu/g.
Example 12
The present embodiment provides a ferment bath composition, including the following raw materials:
180kg of pine sawdust, 150kg of cypress sawdust, 180kg of rice bran, 22kg of corn, 0.12kg of yeast, 0.18kg of bacillus subtilis, 0.08kg of bacillus coagulans, 0.04kg of lactic acid bacteria and 60kg of water.
Wherein the bacteria content of the microzyme is 200 hundred million cfu/g, the bacteria content of the bacillus subtilis is 1000 hundred million cfu/g, the bacteria content of the bacillus coagulans is 100 hundred million cfu/g, and the bacteria content of the lactic acid bacteria is 120 hundred million cfu/g.
Examples 1-12 provide methods of preparing enzyme bath compositions comprising the steps of:
the raw materials of each example were mixed in a ferment bath fermenter and fermented at 55 ℃ for 24 hours to obtain a ferment bath composition.
Example 13
This example provides a ferment bath composition, which has the same composition and amount of raw materials as those in example 1.
The preparation method of the enzyme bath composition provided in this embodiment includes the following steps:
putting pine sawdust, rice bran, corn, saccharomycetes, bacillus subtilis, bacillus coagulans and water in a formula ratio into a ferment bath fermentation tank, mixing, and fermenting at the temperature of 66 ℃ for 48 hours to obtain the ferment bath composition.
Comparative example 1
This comparative example provides an enzyme bath composition, which was the same as example 1 except that the amount of yeast in the raw materials was changed to 0.5 kg.
Comparative example 2
This comparative example provides an enzyme bath composition, which was the same as example 1 except that the amount of yeast in the raw materials was changed to 2.0 kg.
Comparative example 3
This comparative example provides an enzyme bath composition, which was the same as example 1 except that the amount of bacillus subtilis in the raw materials was replaced with 1.0 kg.
Comparative example 4
This comparative example provides an enzyme bath composition, which was the same as in example 1 except that the amount of bacillus subtilis in the raw materials was changed to 2.5 kg.
Comparative example 5
This comparative example provides an enzyme bath composition, which was the same as in example 1 except that the amount of bacillus coagulans in the raw materials was changed to 0.5 kg.
Comparative example 6
This comparative example provides an enzyme bath composition, which was the same as in example 1 except that the amount of bacillus coagulans in the raw materials was replaced with 1.5 kg.
Comparative example 7
This comparative example provides an enzyme bath composition, which is the same as example 1 except that yeast among raw materials is removed.
Comparative example 8
This comparative example provides an enzyme bath composition, which was the same as in example 1 except that bacillus subtilis was removed from the raw materials.
Comparative example 9
This comparative example provides a ferment bath composition, except that bacillus coagulans in the raw material was removed, the other raw materials and the amount were the same as example 1.
Comparative example 10
This comparative example provides an enzyme bath composition, which was the same as example 1 except that yeast and bacillus subtilis among the raw materials were simultaneously removed.
Comparative example 11
This comparative example provides an enzyme bath composition, which was the same as example 1 except that bacillus subtilis and bacillus coagulans were simultaneously removed from the raw materials.
Comparative example 12
This comparative example provides an enzyme bath composition, which was the same as example 1 except that yeast and bacillus coagulans were simultaneously removed from the raw materials, and the other raw materials and amounts were the same.
Comparative example 13
This comparative example provides an enzyme bath composition comprising the following raw materials:
350kg of pine sawdust, 100kg of rice bran powder, 15kg of corn flour, 0.5kg of yeast, 0.2kg of bacillus subtilis, 0.1kg of bacillus coagulans and 60kg of water;
wherein the bacteria content of the microzyme is 200 hundred million cfu/g, the bacteria content of the bacillus subtilis is 1000 hundred million cfu/g, and the bacteria content of the bacillus coagulans is 100 hundred million cfu/g.
Comparative example 14
This comparative example provides an enzyme bath composition comprising the following raw materials:
350kg of pine sawdust, 150kg of rice bran powder, 20kg of corn flour, 0.18kg of yeast, 0.10kg of bacillus subtilis, 0.05kg of bacillus coagulans and 60kg of water;
wherein the bacteria content of the microzyme is 200 hundred million cfu/g, the bacteria content of the bacillus subtilis is 1000 hundred million cfu/g, and the bacteria content of the bacillus coagulans is 100 hundred million cfu/g.
In order to verify the technical effects of the above-described examples and comparative examples, the following experimental examples were specifically set forth.
Experimental example 1
The odor of the ferment bath compositions provided in examples 1-13 and comparative examples 1-14, and the organic content and nitrogen content of the ferment bath compositions before and after use were measured according to NY525-2012, and the use of the ferment bath compositions provided in each example and comparative example included the following steps: burying the human body in the enzyme bath composition for 15 min. Specific results are shown in table 1.
TABLE 1
As can be seen from the data in table 1, the enzyme bath compositions provided in the examples of the present invention have no odor and have high organic matter and nitrogen content before use. After the ferment bath composition is used, the organic matter content and the nitrogen content of the ferment bath composition provided by each embodiment are lower than those of the comparative examples, which shows that microbial strains (saccharomycetes, bacillus subtilis and odor removing bacteria) in the ferment bath composition fully decompose and metabolize raw materials such as base materials, rice bran and corn in the ferment bath composition to generate beneficial small molecular substances such as ferment, and when a human body is subjected to ferment bath, the high temperature generated by the ferment bath enables the human body to freely perspire, so that the beneficial small molecular substances such as ferment can be absorbed while the toxin in the body is discharged out of the body through pores in the skin of the human body, and the metabolism of the human body can be accelerated.
Experimental example 2
The enzyme bath compositions provided in examples 1 to 13 were applied to an enzyme bath, and the body condition of a human body after the enzyme bath was performed was examined.
Human hair can grow 0.8-1.2 cm per month before human body uses ferment bath composition to carry out the ferment bath, and human hair can grow 1.5-2.0 cm per month after human body uses ferment bath composition to carry out the ferment bath, and this is mainly because after the ferment bath, human metabolism is accelerated to the growth of hair has been accelerated.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (15)
1. The enzyme bath composition is characterized by comprising the following raw materials in parts by weight:
3000-3500 parts of base material, 1500-2000 parts of rice bran, 200-250 parts of corn, 1.0-1.5 parts of yeast, 1.5-2.0 parts of bacillus subtilis, 0.8-1.2 parts of odor removing bacteria and 500-800 parts of water;
the base material comprises pine sawdust and/or cypress sawdust; the rice bran is rice bran powder; the corn is corn flour;
the bacteria content of the microzyme is 100-200 hundred million cfu/g;
the bacterium content of the bacillus subtilis is 800-1200 hundred million cfu/g;
the odor removing bacteria comprise bacillus coagulans and optional lactic acid bacteria, and the bacteria content of the odor removing bacteria is 80-120 hundred million cfu/g.
2. The ferment bath composition of claim 1, comprising the following raw materials in parts by weight:
base material 3020-.
3. The ferment bath composition of claim 1, comprising the following raw materials in parts by weight:
3050-3450 parts of base material, 1550-1950 parts of rice bran powder, 245 parts of corn flour 210-245 parts of yeast, 1.2-1.4 parts of bacillus subtilis, 1.0-1.2 parts of odor removing bacteria and 510-790 parts of water.
4. The enzyme bath composition according to any one of claims 1 to 3, wherein the rice bran powder has a mesh size of 60 to 80 mesh.
5. The ferment bath composition of any one of claims 1 to 3, wherein the corn meal has a mesh size of 100-120 meshes.
6. The ferment bath composition according to any one of claims 1 to 3, wherein the raw materials of the ferment bath composition further comprise Bacillus licheniformis, and the weight portion of the Bacillus licheniformis is 0.5-1.0 portion.
7. The ferment bath composition of claim 6, wherein the bacillus licheniformis bacteria content is 800-.
8. The ferment bath composition according to any one of claims 1 to 3, wherein the ferment bath composition has a content of organic matter of 75-85% by mass.
9. The ferment bath composition according to any one of claims 1 to 3, wherein the nitrogen content of the ferment bath composition is 3 to 7% by mass.
10. The method of preparing the ferment bath composition of any one of claims 1-9, comprising the steps of:
mixing the base material, rice bran powder, corn flour, saccharomycetes, bacillus subtilis, odor removing bacteria, water and optionally bacillus licheniformis according to the formula ratio, and fermenting to obtain the enzyme bath composition.
11. The method of preparing the ferment bath composition of claim 10, wherein the temperature of fermentation is 55-70 ℃.
12. The method of preparing the ferment bath composition of claim 11, wherein the temperature of fermentation is 56-68 ℃.
13. The method of preparing the ferment bath composition of claim 11, wherein the fermentation time is 24-48 hours.
14. The method of preparing the ferment bath composition of claim 11, wherein the fermentation time is 25-45 hours.
15. Use of the ferment bath composition of any one of claims 1 to 9 or the ferment bath composition produced by the method of any one of claims 10 to 14 for the preparation of a ferment bath.
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| CN101897659A (en) * | 2010-08-05 | 2010-12-01 | 全然酵素科技发展(大连)有限公司 | Method for preparing enzyme warm bath material by probiotic solid fermentation technology |
| CN104856923A (en) * | 2015-04-24 | 2015-08-26 | 西藏月王生物技术有限公司 | Cordycep enzyme bath combination and fermentation production and application thereof |
| CN106943538A (en) * | 2017-03-14 | 2017-07-14 | 李春岭 | Nano biological ferment bath composition and its preparation method and application |
| CN107693551A (en) * | 2017-08-25 | 2018-02-16 | 山东凯漫健康咨询有限公司 | Compound ferment bath composition and its fermentation process and application |
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| CN101897659A (en) * | 2010-08-05 | 2010-12-01 | 全然酵素科技发展(大连)有限公司 | Method for preparing enzyme warm bath material by probiotic solid fermentation technology |
| CN104856923A (en) * | 2015-04-24 | 2015-08-26 | 西藏月王生物技术有限公司 | Cordycep enzyme bath combination and fermentation production and application thereof |
| CN106943538A (en) * | 2017-03-14 | 2017-07-14 | 李春岭 | Nano biological ferment bath composition and its preparation method and application |
| CN107693551A (en) * | 2017-08-25 | 2018-02-16 | 山东凯漫健康咨询有限公司 | Compound ferment bath composition and its fermentation process and application |
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