CN111157723B - Novel coronavirus rapid detection box - Google Patents
Novel coronavirus rapid detection box Download PDFInfo
- Publication number
- CN111157723B CN111157723B CN202010094652.1A CN202010094652A CN111157723B CN 111157723 B CN111157723 B CN 111157723B CN 202010094652 A CN202010094652 A CN 202010094652A CN 111157723 B CN111157723 B CN 111157723B
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- cavity
- detection
- sample adding
- sealing
- sampling
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/165—Coronaviridae, e.g. avian infectious bronchitis virus
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2469/00—Immunoassays for the detection of microorganisms
- G01N2469/10—Detection of antigens from microorganism in sample from host
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2469/00—Immunoassays for the detection of microorganisms
- G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a novel rapid detection box for coronaviruses, wherein when sample is added, external threads on a thread section are in threaded connection with internal threads on a sealing sleeve to form a layer of sealing protection, and an annular sealing cover can seal the joint again to improve the tightness; along with the screwing of the screw thread fit, the inclined surface of the abutting section can push the hemispherical valve to move downwards, so that the sampling smoothly enters the flow guiding assembly. The invention also provides the inactivating cavity and the fire-extinguishing alcohol stored in the handle part, after detection is finished, the inactivating cavity is extruded to break the flexible membrane, inactivating liquid in the inactivating cavity can enter the sampling cavity and the sampling tube to inactivate residual sampling along the passage, and the inactivating alcohol can inactivate viruses in the detection cavity, so that the existence of the viruses is avoided, and people can discard the viruses after detection with confidence.
Description
Technical Field
The invention relates to the technical field of virus detection equipment, in particular to a novel rapid detection box for coronaviruses.
Background
Currently, a "fluorescent PCR (polymerase chain reaction) method" is mainly used for detecting a novel coronavirus, i.e., a virus RNA is reverse transcribed into DNA, and a trace amount of DNA is greatly amplified by the polymerase chain reaction, thereby detecting a virus with a specific gene fragment. This process requires a sophisticated assay instrument and stringent operating requirements, which are difficult for the general public to detect at home.
Although there are also detection boxes in the form of colloidal gold test paper on the market, which can satisfy the self-detection of the public at home, the existing detection boxes in the form of colloidal gold test paper have the following problems: firstly, during the process of collecting a sample in a sample adding hole, the sample may leak, so that viruses enter the air to form aerosol, and the risk of infection is caused to other personnel; secondly, most of the existing detection boxes in the form of colloidal gold test paper are disposable detection boxes, and after detection, viruses which are not inactivated possibly remain in the detection boxes, and secondary infection can be caused after random discarding; in addition, the existing colloidal gold test paper type detection box is single in detection form, namely, antibodies or antigens are detected, and in different periods when viruses enter human bodies, antibodies and antigens can be in different amounts, and the detection results can be inaccurate due to different detection periods.
Disclosure of Invention
Aiming at the defects in the technology, the invention provides a novel rapid detection box for coronaviruses.
The technical scheme adopted for solving the technical problems is as follows:
a novel rapid assay cassette for coronaviruses comprising:
two detection box units and two sample adding components which are connected side by side, wherein one detection box unit and one corresponding sample adding component form an antibody detection unit, and the other detection box unit and the corresponding sample adding component form an antigen detection unit;
the detection box unit comprises a sealing shell, a handle part is formed at one end of the sealing shell, a detection cavity is formed at the other end of the sealing shell, a sample adding hole is formed in the upper part of one end, far away from the handle part, of the detection cavity, a sealing sleeve is formed at the outer side of the sample adding hole, and the sample adding component is in threaded connection with the sealing sleeve;
a flow guide assembly is fixedly arranged in one end of the detection cavity, which is close to the handle part, one end of the flow guide assembly is connected with the sample adding hole, the other end of the flow guide assembly is connected with the reagent bearing part, an observation window is further arranged on the sealed shell, and the observation window is positioned right above the reagent bearing part; the handle part is internally provided with the deactivated alcohol which can be controlled and released.
Preferably, the handle portion and the detection cavity are isolated by a sealing diaphragm arranged inside the sealing shell, fire extinguishing alcohol is stored in the handle portion, and a cutting portion for cutting the sealing diaphragm to release the fire extinguishing alcohol to the detection cavity is accommodated in the handle portion.
Preferably, the cutting part comprises a push rod, one end of the push rod extends out of the sealing shell, the other end of the push rod is fixedly connected with a blade, and the blade extends towards the sealing diaphragm; the inside fixed mounting of handle portion has the riser, the push rod passes the riser, just the cover is equipped with the spring on the outer circumference of push rod, the one end of spring with the push rod is fixed, the other end supports and leans on the riser.
Preferably, a push handle is installed at one end of the push rod extending out of the sealing shell, and a positioning pin is inserted on the push rod between the push handle and the sealing shell.
Preferably, a guide groove is further formed in the handle portion, and the blade is guided to move through the guide groove.
Preferably, an internal thread is arranged on the inner wall of the sealing sleeve, an external thread is formed at the lower part of the sample adding component, and the internal thread and the external thread form threaded fit.
Preferably, the lower part of the sample adding hole is fixedly provided with a flexible part extending towards the detection cavity, a half ball valve is arranged below the flexible part, and the lower part of the half ball valve is connected with the bottom wall of the detection cavity through a compression spring.
Preferably, the sample adding assembly comprises an inactivation cavity, a sampling cavity and a sample adding pipe which are sequentially arranged from top to bottom, wherein a flexible membrane is arranged between the inactivation cavity and the sampling cavity, and the sampling cavity is communicated with a flow passage of the sample adding pipe; the inactivation cavity is internally provided with inactivation liquid, and the sampling cavity is internally provided with sampling.
Preferably, the sampling tube comprises a direct current section, a thread section and an abutting section which are sequentially arranged from top to bottom, wherein an external thread is arranged on the outer wall of the thread section, and the external thread is in threaded connection with an internal thread on the sealing sleeve.
Preferably, an inclined plane is formed at the lower part of the abutting section, dripping scale marks are arranged on the outer side of the direct current section, and an annular sealing cover is arranged at the joint of the thread section and the direct current section.
Compared with the prior art, the invention has the beneficial effects that: the novel rapid coronavirus detection box provided by the invention has the advantages that the two detection box units and the two sample adding components respectively form the antigen detection unit and the antibody detection unit, and the antigen and the antibody can be detected simultaneously by setting different detection reagents and selecting different detection samples; the semi-ball valve is arranged, so that when sample addition detection is not performed, the semi-ball valve can also be abutted against the flexible piece to seal the sample addition hole, and subsequent detection failure caused by the fact that foreign matters enter the detection box unit is avoided; when the sample is added, the external thread on the thread section is in threaded connection with the internal thread on the sealing sleeve to form a layer of sealing protection, and the annular sealing cover can seal the joint again to improve the tightness; along with the screwing of the screw thread fit, the inclined surface of the abutting section can push the hemispherical valve to move downwards, so that the sampling smoothly enters the flow guiding assembly. The invention also provides the inactivating cavity and the fire-extinguishing alcohol stored in the handle part, after detection is finished, the inactivating cavity is extruded to break the flexible membrane, inactivating liquid in the inactivating cavity can enter the sampling cavity and the sampling tube to inactivate residual sampling along the passage, and the inactivating alcohol can inactivate viruses in the detection cavity, so that the existence of the viruses is avoided, and people can discard the viruses after detection with confidence.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is a schematic diagram of the structure of a cartridge unit according to the present invention;
FIG. 3 is a schematic view showing the internal structure of the cartridge unit of the present invention;
FIG. 4 is a top view showing the internal structure of the cartridge unit according to the present invention;
FIG. 5 is a side view of a cartridge unit of the present invention;
FIG. 6 is a schematic diagram of the sample loading assembly of the present invention;
FIG. 7 is a cross-sectional view of the loading assembly of the present invention.
In the figure: 1. the device comprises a detection box unit 2, a sample adding assembly 3, a flow guiding assembly 4, a reagent bearing part 10, a sealing shell 11, a detection cavity 12, a handle part 13 and a half ball valve; 14. the device comprises a vertical plate, 15, a cutting part, 16, a locating pin, 17, a spring, 18, a guide groove, 100, a display window, 101, a sealing sleeve, 102, a sample adding hole, 103 and internal threads; 120. sealing diaphragm, 131, compression spring, 151, push rod, 152, blade, 153, push handle, 224, external screw thread, 23, inactivation chamber, 21, sampling chamber, 22, sampling tube, 231, flexible diaphragm, 220, flow path, 223, direct current section, 222, screw thread section, 221, abutting section, 25, drop scale mark, 24, annular seal cover.
Detailed Description
The present invention is described in further detail below with reference to the drawings to enable those skilled in the art to practice the invention by referring to the description.
As shown in fig. 1 to 7, the present invention provides a novel rapid detection kit for coronaviruses, comprising: two detection box units 1 and two sample adding components 2 which are connected side by side, wherein one detection box unit 1 and one corresponding sample adding component 2 form an antibody detection unit, and the other detection box unit 1 and the corresponding sample adding component 2 form an antigen detection unit; the detection box unit 1 comprises a sealed shell 10, wherein a handle part 12 is formed at one end of the sealed shell 10, a detection cavity 11 is formed at the other end of the sealed shell, a sample adding hole 102 is formed in the upper part of one end, far away from the handle part 12, of the detection cavity 11, a sealed sleeve 101 is formed on the outer side of the sample adding hole 102, and the sample adding assembly 2 is in threaded connection with the sealed sleeve 101; a flow guide assembly 3 is fixedly arranged in one end of the detection cavity 11, which is close to the handle part 12, one end of the flow guide assembly 3 is connected with the sample adding hole 102, the other end of the flow guide assembly is connected with the reagent bearing part 4, an observation window 100 is further arranged on the sealed shell 10, and the observation window 100 is positioned right above the reagent bearing part 4; the handle portion 12 contains a releasably controlled inactivating alcohol.
Through the arrangement, when virus detection is carried out, the sample adding assembly 2 and the sample adding hole 102 can be connected through threads to form a seal, so that the leakage of viruses in the sample is avoided; the two detection box units 1 and the two sample adding components 2 respectively form an antigen detection unit and an antibody detection unit, and antigen and antibody can be detected simultaneously by setting different detection reagents and selecting different detection samples, for example, in the early stage of virus entering a human body, the number of the antigen is relatively more, and the number of the antibody is less, and at the moment, whether the virus exists or not can be judged through the reaction of the antigen detection reagent and the virus in saliva; the antibody is correspondingly generated when the virus quantity is increased along with the time of entering the human body, and the antibody can be detected while the antigen is detected, so that the detection accuracy is ensured, and further, the evidence can be provided for the estimation of the virus infection stage, so that different antiviral drugs can be taken in different infection stages.
As an embodiment of the present invention, the handle portion 12 and the detection chamber 11 are isolated by a sealing diaphragm 120 disposed inside the sealed housing 10, fire extinguishing alcohol is stored in the handle portion 12, and a cutting portion 15 for cutting the sealing diaphragm 120 to release the fire extinguishing alcohol to the detection chamber 11 is accommodated.
The cutting part 15 comprises a push rod 151, one end of the push rod 151 extends out of the sealing shell 10, the other end of the push rod is fixedly connected with a blade 152, and the blade 152 extends towards the sealing diaphragm 120; the handle 12 is fixedly provided with a vertical plate 14 inside, the push rod 151 penetrates through the vertical plate 14, a spring 17 is sleeved on the outer circumference of the push rod 151, one end of the spring 17 is fixed with the push rod 151, and the other end of the spring abuts against the vertical plate 14.
Further, a push handle 153 is mounted at one end of the push rod 151 extending out of the sealing housing 10, and a positioning pin 16 is inserted on the push rod 151 between the push handle 153 and the sealing housing 10.
Further, a guide groove 18 is provided in the handle portion 12, and the blade 152 is guided to move via the guide groove 18.
As an embodiment of the present invention, an internal thread 103 is provided on the inner wall of the sealing sleeve 101, an external thread 224 is formed on the lower portion of the sample loading assembly 2, and the internal thread 103 and the external thread 224 form a threaded fit.
Further, a flexible member 104 extending toward the detection cavity 11 is fixedly installed at the lower portion of the sample loading hole 102, a half ball valve 13 is disposed below the flexible member 104, and the lower portion of the half ball valve 13 is connected with the bottom wall 111 of the detection cavity 11 through a compression spring 131.
Through above-mentioned setting for when not carrying out the application of sample and detecting, the hemisphere valve also can support and lean on the flexible piece, seals the application of sample hole, avoids external material to get into the detection box unit, and leads to subsequent detection inefficacy.
As an embodiment of the present invention, the sample adding assembly 2 includes an inactivation chamber 23, a sampling chamber 21, and a sample adding tube 22 sequentially disposed from top to bottom, wherein a flexible membrane 231 is disposed between the inactivation chamber 23 and the sampling chamber 21, and the sampling chamber 21 is communicated with a flow path 220 of the sample adding tube 22; the inactivating liquid is stored in the inactivating chamber 23, and the sample is stored in the sample chamber 21.
The sampling tube 22 comprises a direct current section 223, a threaded section 222 and an abutting section 221 which are sequentially arranged from top to bottom, wherein an external thread 224 is arranged on the outer wall of the threaded section 222, and the external thread 224 is in threaded connection with an internal thread on the sealing sleeve 101.
Further, an inclined plane is formed at the lower part of the abutting section 221, a drip scale line 25 is arranged at the outer side of the direct current section 223, and an annular sealing cover 24 is arranged at the joint of the thread section 222 and the direct current section 223.
When the sample is added, the external thread on the thread section is in threaded connection with the internal thread on the sealing sleeve to form a layer of sealing protection, and the annular sealing cover can seal the joint again to improve the tightness; along with the screwing of the screw thread fit, the inclined surface of the abutting section can push the hemispherical valve to move downwards, so that the sampling smoothly enters the flow guiding assembly. The invention also provides an inactivation cavity, after detection is finished, the flexible membrane is broken by extruding the inactivation cavity, and the inactivation liquid in the inactivation cavity can enter the sampling cavity and the sampling tube to inactivate residual samples along the passage, so that the existence of viruses is further avoided.
Although embodiments of the present invention have been disclosed above, it is not limited to the details and embodiments shown and described, it is well suited to various fields of use for which the invention would be readily apparent to those skilled in the art, and accordingly, the invention is not limited to the specific details and illustrations shown and described herein, without departing from the general concepts defined in the claims and their equivalents.
Claims (7)
1. A novel rapid assay cassette for coronaviruses comprising:
two detection box units (1) and two sample adding components (2) which are connected side by side, wherein one detection box unit (1) and one corresponding sample adding component (2) form an antibody detection unit, and the other detection box unit (1) and the corresponding sample adding component (2) form an antigen detection unit;
the detection box unit (1) comprises a sealing shell (10), a handle part (12) is formed at one end of the sealing shell (10), a detection cavity (11) is formed at the other end of the sealing shell, a sample adding hole (102) is formed in the upper part of one end, far away from the handle part (12), of the detection cavity (11), a sealing sleeve (101) is formed outside the sample adding hole (102), and the sample adding assembly (2) is in threaded connection with the sealing sleeve (101);
a flow guide assembly (3) is fixedly arranged in one end, close to the handle part (12), of the detection cavity (11), one end of the flow guide assembly (3) is connected with the sample adding hole (102), the other end of the flow guide assembly is connected with the reagent bearing part (4), an observation window (100) is further arranged on the sealed shell (10), and the observation window (100) is positioned right above the reagent bearing part (4); the handle part (12) is internally provided with the releasable controlled inactivated alcohol;
the handle part (12) and the detection cavity (11) are isolated by a sealing diaphragm (120) arranged inside the sealing shell (10), the handle part (12) stores inactivated alcohol, and a cutting part (15) for cutting the sealing diaphragm (120) to release the inactivated alcohol to the detection cavity (11) is accommodated;
the cutting part (15) comprises a push rod (151), one end of the push rod (151) extends out of the sealing shell (10), the other end of the push rod is fixedly connected with a blade (152), and the blade (152) extends towards the sealing diaphragm (120); a vertical plate (14) is fixedly arranged in the handle part (12), the push rod (151) passes through the vertical plate (14), a spring (17) is sleeved on the outer circumference of the push rod (151), one end of the spring (17) is fixed with the push rod (151), and the other end of the spring is abutted against the vertical plate (14); the sample adding assembly (2) comprises an inactivation cavity (23), a sampling cavity (21) and a sample adding pipe (22) which are sequentially arranged from top to bottom, wherein a flexible membrane (231) is arranged between the inactivation cavity (23) and the sampling cavity (21), and the sampling cavity (21) is communicated with a flow passage (220) of the sample adding pipe (22); the inactivation cavity (23) is internally provided with inactivation liquid, and the sampling cavity (21) is internally provided with sampling.
2. The rapid detection box for novel coronaviruses according to claim 1, wherein a push handle (153) is arranged at one end of the push rod (151) extending out of the sealing shell (10), and a positioning pin (16) is inserted on the push rod (151) between the push handle (153) and the sealing shell (10).
3. The rapid detection kit for novel coronaviruses according to claim 1, wherein a guiding groove (18) is further provided in the handle portion (12), and the blade (152) is guided to move via the guiding groove (18).
4. The rapid detection box for novel coronaviruses according to claim 1, wherein an inner thread (103) is arranged on the inner wall of the sealing sleeve (101), an outer thread (224) is formed on the lower part of the sample adding component (2), and the inner thread (103) and the outer thread (224) form a threaded fit.
5. The rapid detection box for novel coronaviruses according to claim 1, wherein a flexible member (104) extending towards the detection cavity (11) is fixedly arranged at the lower part of the sample adding hole (102), a half ball valve (13) is arranged below the flexible member (104), and the lower part of the half ball valve (13) is connected with the bottom wall (111) of the detection cavity (11) through a compression spring (131).
6. The rapid detection box for the novel coronavirus according to claim 1, wherein the sampling tube (22) comprises a direct current section (223), a thread section (222) and an abutting section (221) which are sequentially arranged from top to bottom, wherein an external thread (224) is arranged on the outer wall of the thread section (222), and the external thread (224) is in threaded connection with an internal thread on the sealing sleeve (101).
7. The rapid detection box for novel coronaviruses according to claim 6, wherein an inclined plane is formed at the lower part of the abutting section (221), a dripping scale mark (25) is arranged at the outer side of the direct current section (223), and an annular sealing cover (24) is arranged at the joint of the threaded section (222) and the direct current section (223).
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CN202010094652.1A CN111157723B (en) | 2020-02-16 | 2020-02-16 | Novel coronavirus rapid detection box |
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CN202010094652.1A CN111157723B (en) | 2020-02-16 | 2020-02-16 | Novel coronavirus rapid detection box |
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CN111157723B true CN111157723B (en) | 2023-07-04 |
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Families Citing this family (6)
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CN112010966B (en) * | 2020-05-15 | 2021-03-19 | 潍坊医学院 | Monoclonal antibody aiming at non-RBD (radial basis function) region of new coronavirus spike protein and application thereof |
CN111505316A (en) * | 2020-05-22 | 2020-08-07 | 无锡市孚维尔生物医疗科技有限公司 | Production method of novel coronavirus 2019-nCoV antibody spectrum detection kit |
CN111624349A (en) * | 2020-05-22 | 2020-09-04 | 无锡市孚维尔生物医疗科技有限公司 | Novel coronavirus 2019-nCoV antibody spectrum detection test strip |
CN111624348A (en) * | 2020-05-22 | 2020-09-04 | 无锡市孚维尔生物医疗科技有限公司 | Method for detecting novel coronavirus 2019-nCoV through multi-protein joint inspection combination |
CN111879927A (en) * | 2020-07-08 | 2020-11-03 | 蔡竹 | Simple excrement occult blood and helicobacter pylori detection device |
CN114655551B (en) * | 2022-04-01 | 2024-02-13 | 浙江优纳特科学仪器有限公司 | Biological sample transport tank |
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