CN207123533U - Swine disease antigen saliva detection means - Google Patents
Swine disease antigen saliva detection means Download PDFInfo
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- CN207123533U CN207123533U CN201721130114.3U CN201721130114U CN207123533U CN 207123533 U CN207123533 U CN 207123533U CN 201721130114 U CN201721130114 U CN 201721130114U CN 207123533 U CN207123533 U CN 207123533U
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Abstract
It the utility model is related to a kind of swine disease antigen saliva detection means, including detecting box, at least one accommodating chamber is provided with detection box, animal virus detection card is provided with accommodating chamber, animal virus detection card includes nitrocellulose coated film, the bottom of nitrocellulose coated film is pasted with PVC bottom plates, is sequentially provided with gold conjugation pad, T areas, C areas, absorbent material on nitrocellulose coated film from front end to tail end；The front side of detection box is provided with animals sputum collecting device, the saliva collection terminal of animals sputum collecting device is positioned at the outside of detection box, the saliva collection end connection gold conjugation pad of animals sputum collecting device, the rear side for detecting box are provided with expansion link, and detection box is provided with the observation window for appearing T areas and C areas.A kind of detect for antigen viral in quick detection animal saliva is the utility model is related to block, it is very easy to use, simple, efficient.Available for the detection card of the antigen of pig blue-ear disease poison, CSFV, Porcine epidemic diarrhea virus, PRV, pig circular ring virus etc., and other animal pathogen detections for taking blood difficult.
Description
Technical field
The utility model is related to animal-breeding apparatus field, and in particular to it is a kind of be quick on the draw, swine disease easy to use resists
Former saliva detection means.
Background technology
Aquaculture controls epidemic disease to turn into one of important means using vaccinating at present, and daily monitoring is produced after vaccinating
Raw antibody also turns into one of important means of detection incidence, and mechanism is raised fauna antibody in pig farm, vaccary etc.
Periodic monitoring alternatively suitable vaccine, assess immune programme for children, the important means of monitoring colony health status.But antibody
Detection be related to the complexity of detection means, data it is ageing the problems such as, existing detection mode has ELISA detection reagents
Box and two kinds of detection modes of fluorescence quantitative PCR detection.
ELISA kit needs ELIASA, and to have suitable incubation reaction condition, board-washing condition, to operation element
Environment and professional and technical personnel require high.Detection sample also needs to the pre-treatment of complexity in addition, such as gathers blood, separates serum
Deng.The process animal stress reaction of collection blood is very big, and animal injures the safety of sample collector sometimes.When need to detect sample compared with
, it is necessary to detect in batches when more, time-consuming longer (detection 2-3 days are needed when sample is more).
PCR is also known as PCR, is a kind of molecular biology skill for being used to amplify the specific DNA fragmentation of amplification
Art, it is considered as the special DNA replication dna of in vitro, PCR maximum features, is carried out after can micro DNA be significantly increased
Detection.
PCR (PCR) is that 95 DEG C Celsius or so high temperature time variations can become single-stranded in vitro using DNA, low
Primer is combined with single-stranded by the principle of base pair complementarity during temperature (often 60 DEG C or so), then temperature regulating to archaeal dna polymerase most
Suitable reaction temperature (72 DEG C or so), direction composition complementary strand of the archaeal dna polymerase along phosphoric acid to pentose (5 ' -3 ').Based on poly-
The actual PCR instrument of synthase manufacture is exactly a temperature control device, can be in denaturation temperature, renaturation temperature, between elongating temperature well
It is controlled, specific DNA fragmentation is amplified, it is rapid to grasp viral level in sample by DNA track of genes systems.
PCR kit needs PCR instrument, incubation reaction condition, operation element environment and professional and technical personnel, detects sample in addition
Originally the pre-treatment of complexity is also needed to, such as gathers blood.And animal stress reaction is very big during gathering blood sample, sample collector
Safety can not also ensure.The factor for influenceing experimental result after detection has a lot, for example reaction system, reaction system mainly include
Six kinds of fundamentals, i.e. PCR buffer, Mg2+, dNTP, primer, template and enzyme；6 key elements of the above can not all malfunction, otherwise
Influence result of the test accuracy.Cycle for obtaining the data report of accurate quantification it is longer (it is most short also to take 3-5 days time, most
Length takes 10 days).
Therefore a kind of method of detection cultivated animals disease safely, fast and accurately, such as pig is needed to breed and breathe comprehensive
The antigens such as simulator sickness virus (being commonly called as " pig blue-ear disease "), CSFV, PRV, pig circular ring virus, pig epidemic diarrhea
Detection mode.
Utility model content
The purpose of this utility model is to provide a kind of swine disease antigen saliva detection means, realizes inspection safely, fast and accurately
Survey.This device can with pig blue-ear disease poison in quick detection pig saliva, CSFV, PRV, pig circular ring virus it is anti-
Former swine disease antigen saliva, and other animal pathogen detections for taking blood difficult.
The purpose of this utility model is to be achieved through the following technical solutions：
A kind of swine disease antigen saliva detection means, including detection box, the detection box is interior to be provided with least one accommodating chamber, institute
State in accommodating chamber and be provided with pig Viral diagnosis card, the pig Viral diagnosis card includes nitrocellulose coated film, the cellulose nitrate
The bottom of plain coated film is pasted with PVC bottom plates, and collaurum is sequentially provided with from front end to tail end on the nitrocellulose coated film
Pad, T areas, C areas, absorbent material；
The front side of the detection box is provided with animals sputum collecting device, and the saliva collection terminal of the animals sputum collecting device is located at
Detect the outside of box, the saliva collection end connection gold conjugation pad of the animals sputum collecting device, the rear side of the detection box
Provided with expansion link, the detection box is provided with the observation window for appearing T areas and C areas.
In preferred embodiments, if the quantity more than one of the accommodating chamber, gear is provided between identical accommodating chamber
Plate.
In preferred embodiments, the saliva collection end of the animals sputum collecting device, which is provided with, connects whole collaurum knots
The saliva for closing pad collects device.
In preferred embodiments, the front side of the detection box bites provided with stop animal detects the catch of box.
In preferred embodiments, the catch is coated on the outside of animals sputum collecting device, and the catch is can be to
The laminated structure of outer bending.
In preferred embodiments, the catch is provided with folding line.
In preferred embodiments, the inside of the animals sputum collecting device is provided with strip metal skeleton, the strip
The outside parcel gauze layer of metallic framework, and form gauze ball in its saliva collection terminal.
In preferred embodiments, pig blue-ear disease poison, CSFV, PRV, pig circle are coated with the T areas
The viral monoclonal antibody of any one in the animal virus such as circovirus virus, Porcine epidemic diarrhea virus.
The beneficial effects of the utility model are：
The utility model is related to it is a kind of be used for viral device in quick detection animal saliva, can quick detection pig blue-ear disease
One or more animal virus such as poison, CSFV, Porcine epidemic diarrhea virus, PRV, pig circular ring virus resist
Original, it could be applicable to taking the detection of the difficult animal pathogen of blood to could be applicable to the inspection to taking the difficult animal pathogen of blood
Survey (by taking sow as an example：Sow positioning rail is very wide, and build is very big, sow when taking blood operation very inconvenient across fence, and taking blood
If quick mobile, syringe needle easily breaks, and is also easy to hurt the arm of operator), it is very easy to use, simple, efficient.
The purpose of this utility model is just to provide a kind of rapid detection card of zoosis antigen, by gathering living animal saliva
Liquid；Different from traditional blood sampling pattern, live diagnosis, 10-20 minutes quickly can be made while greatly reducing Animal stress
Know the result of qualitative detection, in place of can solving above-mentioned the deficiencies in the prior art, and the supplement hand to above-mentioned technical proposal
Section.
The 4th day after susceptible pig-pig infection indigo plant otopathy poison, CSFV, PRV, pig circular ring virus can be from
Corresponding viral antigen is detected in saliva, and viral level maintains more stable level.So above pig is used as by the use of saliva
The detection sample of virus infection, early detection, the infection of examination swine disease poison and control swine disease prevalence are significant.
Brief description of the drawings
The utility model is described in further detail below according to accompanying drawing.
Fig. 1 is the external structure of the swine disease antigen saliva detection means described in the utility model embodiment 1, wherein catch
6 are in open mode；
Fig. 2 a are the structural representations that catch 6 is in merging phase in Fig. 1；
Fig. 2 b are the structural representations that another catch 6 is in open mode；
Fig. 3 is the overall structure figure of the swine disease antigen saliva detection means described in the utility model embodiment 2.
In figure：
1st, box is detected；2nd, accommodating chamber；3rd, demarcation strip；4th, expansion link；5th, observation window；6th, catch；7th, folding line；
8th, animals sputum collecting device；9th, strip metal skeleton；10th, gauze layer；11st, saliva collects device；
12nd, nitrocellulose coated film；13rd, gold conjugation pad；14th, absorbent material；15th, T areas；16th, C areas.
Embodiment
It is new below in conjunction with this practicality to make the purpose, technical scheme and advantage of the utility model embodiment clearer
Accompanying drawing in type embodiment, the technical scheme in the embodiment of the utility model is clearly and completely described；Obviously, retouched
The embodiment stated is the utility model part of the embodiment, rather than whole embodiments.Generally here described in accompanying drawing and
The component of the utility model embodiment shown a variety of can also be configured to arrange and design.Therefore, below to attached
The detailed description of the embodiment of the present utility model provided in figure is not intended to limit claimed the scope of the utility model,
But it is merely representative of selected embodiment of the present utility model.Based on the embodiment in the utility model, ordinary skill people
The every other embodiment that member is obtained under the premise of creative work is not made, belong to the model of the utility model protection
Enclose.
Embodiment of the present utility model is described below in detail, the example of the embodiment is shown in the drawings, wherein from beginning
Same or similar element is represented to same or similar label eventually or there is the element of same or like function.Below by ginseng
The embodiment for examining accompanying drawing description is exemplary, it is intended to for explaining the utility model, and it is not intended that to the utility model
Limitation.
The utility model is further described with specific embodiment below with reference to accompanying drawings.
Embodiment 1
As shown in figure 1, a kind of swine disease antigen saliva detection means of the utility model embodiment, including detection box 1, it is described
An accommodating chamber 2 is provided with detection box 1, Viral diagnosis card is provided with the accommodating chamber 2, the front side of the detection box 1 is provided with dynamic
Thing spittle collector 8, the inside of the animals sputum collecting device 8 are provided with strip metal skeleton 9, the strip metal skeleton 9
Gauze layer 10 is wrapped up in outside, and forms gauze ball, the saliva collection terminal position of the animals sputum collecting device 8 in its saliva collection terminal
In the outside of detection box 1, the receiving terminal (colloid of the saliva collection end connection pig Viral diagnosis card of the animals sputum collecting device 8
Golden pad 13), the rear side of the detection box 1 is provided with expansion link 4, and expansion link 4 is scalable to be changed between inspector and animal
Safe distance, prevent injury of the animal to inspector, it is ensured that the personal safety of inspector, its material are plastics or metal.Institute
State detection box 1 and be provided with the observation window 5 for appearing T areas 15 and C areas 16, observation window 5 is made up of transparent material.
As shown in Fig. 2 a or 2b, the front side of the detection box 1 bites provided with stop animal detects the catch 6 of box 1, the gear
Piece 6 is laminated structure, is coated on the outside of animals sputum collecting device 8, is at least symmetrical both sides or upper bottom left up and down
On right four sides, the catch 6 for can outward bending, when needing to collect animal saliva by the outward bending of catch 6, its one end
On the edge for riding over detection box 1 front end, catch 6 is formed angled construction, make shape between animals sputum collecting device 8 and detection box 1
It is sloping, if the tooth that animal bites animal behind the slope during the chewing on animal spittle collector 8 can slide into from slope
On animals sputum collecting device 8, while detection box 1 is promoted backward, detection box 1 is moved rearwards so as to remind user to need.Institute
State catch 6 and be provided with folding line 7, be easy to the crooked place fixed on catch 6, the structure on catch 6 elsewhere is made firmly
Structure, can solve catch 6 because it is overall bent and caused by its intensity decreases, can not repeatedly resist asking for animal chew for a long time
Topic.
The pig Viral diagnosis card includes nitrocellulose coated film 12, and the bottom of the nitrocellulose coated film 12 is glued
Post PVC bottom plates, the front end on the nitrocellulose coated film 12 is provided with gold conjugation pad 13, and rear end is provided with absorbent material
14, centre is sequentially provided with T areas 15 from front to back, be coated with the T areas 15 pig blue-ear disease poison, CSFV, PRV,
The viral monoclonal antibody of any one in pig circular ring virus, Porcine epidemic diarrhea virus, the monoclonal antibody of the animal virus
The monoclonal antibody of swine disease poison is not limited only to, the species of virus is coated with according to T areas, it may also be used for other animal virus antigens
Detection, because animal virus species is excessive, does not just enumerate, anti-mouse antibody can be coated with C areas 16 in the description.
Below by detection pig blue-ear disease poison (monoclonal antibody of pig blue-ear disease poison is coated with i.e. in T areas 15, is wrapped in C areas 16
By anti-mouse antibody), illustrate its application method：
Open catch 6 and put up, expose the saliva collection terminal of animals sputum collecting device 8, expansion link 4 is adjusted suitable
Length, expansion link 4 is held, saliva collection terminal is sent on front side of animal oral cavity, chewed using the curious psychology and hobby of pig
Characteristic, pig can actively chew saliva collection terminal, and saliva will be entered in animals sputum collecting device 8 by siphonage, and be sent to disease
On poison detection card.
If it is malicious (PRRSV) not contain pig blue-ear disease in saliva sample, only C areas 16 are presented color developing effect, at T areas 15
There is no color to show.
If malicious (PRRSV) containing pig blue-ear disease in saliva sample, T areas 15 and C areas 16 are each that color developing effect is presented.
If C areas 16 occur without red line, then it represents that detection is invalid, it is necessary to re-replace detection card.
Embodiment 2
It is a variety of viral in order to solve the problems, such as to detect simultaneously, it can will detect the quantity set in box 1 with a variety of viral species
Identical accommodating chamber 2, it is sick in pig blue-ear disease poison, CSFV, PRV, pig circular ring virus four can detect simultaneously
Poison is embodiment, and its concrete technical scheme is as follows：
As shown in figure 3, a kind of swine disease antigen saliva detection means, including detection box 1, the detection box 1 is interior to be provided with four
Accommodating chamber 2, different Viral diagnosis cards is placed respectively in four accommodating chambers 2, pig blue-ear disease poison can be placed in this embodiment
Detection card, CSFV detection card, PRV detect card, pig circular ring virus detection card, are provided between adjacent accommodating chamber 2
Demarcation strip 3, for separating each accommodating chamber 2, the front side of the detection box 1 is provided with animals sputum collecting device 8, above-mentioned four kinds of diseases
Poison detection card is identical with the structure illustrated in embodiment 1 with the structure of animals sputum collecting device 8.
The saliva collection terminal of the animals sputum collecting device 8 is positioned at the outside of detection box 1, the animals sputum collecting device 8
Saliva collection end connection saliva collect device 11, saliva collects the hollow cylinder structure made by gauze layer 10 of device 11, and its is hollow
Locate to be used to collect animal saliva, saliva collects the gold conjugation pad 13 that device 11 connects above-mentioned four kinds of Viral diagnosis cards, for inciting somebody to action
The animal saliva that animals sputum collecting device 8 collects is transported on four kinds of Viral diagnosis cards by siphonage, the detection box 1
Rear side be provided with expansion link 4, the detection box 1 is provided with the observation window 5 in the T areas 15 and C areas 16 that appear four Viral diagnosis cards,
Observation window 5 is made up of transparent material.
Its application method is specifically described below：
Open catch 6 and put up, expose the saliva collection terminal of animals sputum collecting device 8, expansion link 4 is adjusted suitable
Length, expansion link 4 is held, saliva collection terminal is sent on front side of animal oral cavity, chewed using the curious psychology and hobby of pig
Characteristic, pig can actively chew saliva collection terminal, and saliva will be entered in animals sputum collecting device 8 by siphonage, reach saliva
Assemble after collecting device 11, saliva can collect device 11 from saliva and overflow after saliva collection amount satisfaction, be sent to four kinds of Viral diagnosis cards
On.
If appointing in pig blue-ear disease poison, CSFV, PRV, pig circular ring virus is not contained in saliva sample
When anticipating a kind of, then color developing effect is presented in the C areas 16 of four detection cards, and with being free of the T areas 15 of viral species in saliva sample
Locate no color to show, conversely, color developing effect is presented at T areas 15.
If four detection Ka Zhongyou C areas 16 occur without red line, then it represents that this detection road is invalid, it is necessary to re-replace
Viral diagnosis card in the accommodating chamber 2.
Finally it should be noted that：Above-described each embodiment is merely to illustrate the technical solution of the utility model, rather than
It is limited；Although the utility model is described in detail with reference to the foregoing embodiments, one of ordinary skill in the art
It should be understood that：It can still modify to the technical scheme described in previous embodiment, or to which part or whole
Technical characteristic carries out equivalent substitution；And these modifications or substitutions, the essence of appropriate technical solution is departed from the utility model
The scope of each embodiment technical scheme.
Claims (8)
1. a kind of swine disease antigen saliva detection means, including detection box, it is characterised in that：It is provided with the detection box at least one
Accommodating chamber, the accommodating chamber is interior to be provided with animal virus detection card, and the animal virus detection, which blocks, includes nitrocellulose coated film,
The bottom of the nitrocellulose coated film is pasted with PVC bottom plates, on the nitrocellulose coated film from front end to tail end according to
It is secondary to be provided with gold conjugation pad, T areas, C areas, absorbent material；
The front side of the detection box is provided with animals sputum collecting device, and the saliva collection terminal of the animals sputum collecting device is positioned at detection
The outside of box, the saliva collection end connection gold conjugation pad of the animals sputum collecting device, the rear side of the detection box are provided with
Expansion link, the detection box are provided with the observation window for appearing T areas and C areas.
2. swine disease antigen saliva detection means according to claim 1, it is characterised in that：If the quantity of the accommodating chamber is more
In 1, then baffle plate is provided between identical accommodating chamber.
3. swine disease antigen saliva detection means according to claim 2, it is characterised in that：The animals sputum collecting device
Saliva collection end is provided with the saliva collection device for connecting whole gold conjugation pads.
4. swine disease antigen saliva detection means according to claim 1, it is characterised in that：The front side of the detection box is provided with
Stop that animal bites and detect the catch of box.
5. swine disease antigen saliva detection means according to claim 4, it is characterised in that：The catch is coated on animal saliva
The outside of liquid collector, the catch for can outward bending laminated structure.
6. swine disease antigen saliva detection means according to claim 5, it is characterised in that：The catch is provided with bending
Line.
7. swine disease antigen saliva detection means according to claim 5, it is characterised in that：The animals sputum collecting device
Inside is provided with strip metal skeleton, the outside parcel gauze layer of the strip metal skeleton, and forms yarn in its saliva collection terminal
Cloth ball.
8. swine disease antigen saliva detection means according to claim 1, it is characterised in that：Pig indigo plant ear is coated with the T areas
The list of any one animal virus in virus, CSFV, PRV, pig circular ring virus, Porcine epidemic diarrhea virus
Clonal antibody.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721130114.3U CN207123533U (en) | 2017-09-05 | 2017-09-05 | Swine disease antigen saliva detection means |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721130114.3U CN207123533U (en) | 2017-09-05 | 2017-09-05 | Swine disease antigen saliva detection means |
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| Publication Number | Publication Date |
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| CN207123533U true CN207123533U (en) | 2018-03-20 |
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ID=61602745
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| CN201721130114.3U Active CN207123533U (en) | 2017-09-05 | 2017-09-05 | Swine disease antigen saliva detection means |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108732347A (en) * | 2018-05-23 | 2018-11-02 | 江苏维尔生物科技有限公司 | A kind of detection kit and preparation method thereof detecting HCV antibody in saliva |
| CN111157723A (en) * | 2020-02-16 | 2020-05-15 | 广西中医药大学附属瑞康医院 | Novel rapid detection kit for coronavirus |
| CN112754535A (en) * | 2021-01-20 | 2021-05-07 | 东北农业大学 | Pig saliva collection system |
-
2017
- 2017-09-05 CN CN201721130114.3U patent/CN207123533U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108732347A (en) * | 2018-05-23 | 2018-11-02 | 江苏维尔生物科技有限公司 | A kind of detection kit and preparation method thereof detecting HCV antibody in saliva |
| CN111157723A (en) * | 2020-02-16 | 2020-05-15 | 广西中医药大学附属瑞康医院 | Novel rapid detection kit for coronavirus |
| CN111157723B (en) * | 2020-02-16 | 2023-07-04 | 广西中医药大学附属瑞康医院 | Novel coronavirus rapid detection box |
| CN112754535A (en) * | 2021-01-20 | 2021-05-07 | 东北农业大学 | Pig saliva collection system |
| CN112754535B (en) * | 2021-01-20 | 2022-06-03 | 东北农业大学 | Pig saliva collection system |
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