CN111157418B - Test device and method for simulating construction of ecological channel based on microbial mineralization - Google Patents
Test device and method for simulating construction of ecological channel based on microbial mineralization Download PDFInfo
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Abstract
The invention discloses an indoor test device and method for simulating construction of an ecological channel based on microbial mineralization, and belongs to the technical field of geotechnical engineering. The device is including water supply system, ecological channel test system and the measurement system who connects gradually: the water supply system is used for providing stable flow and simulating channel water flow; the ecological channel test system is used for simulating the running state of a channel under different microorganism mineralization materials and processes; the measuring system is used for testing the permeability, the erosion resistance and the strength of the soil body before and after the microbial mineralization treatment. The method comprises the following steps: and sampling and placing in a soil sample box, processing different microbial mineralization materials and construction processes, testing the permeability, scour resistance and strength of the processed soil body, and analyzing and determining the materials and construction processes for building the ecological channel based on microbial mineralization. The invention can test the permeability, the scour resistance and the strength of the soil before and after the microbial mineralization treatment, and develop the technology for building ecological channels based on the microbial mineralization.
Description
The technical field is as follows:
the invention belongs to the technical field of geotechnical engineering, and particularly relates to a test device and a method for simulating the construction of an ecological channel based on microbial mineralization.
Background art:
channel engineering is a small-sized water conservancy facility for irrigation and drainage of farmlands, and can be generally divided into an excavation channel and a filling channel. Because the natural soil body has lower strength and poorer impermeability and scour resistance, a concrete lining is generally required to be arranged on the surface of the channel soil body in the prior engineering, one of the main raw materials is cement, and a large amount of energy is consumed and greenhouse gas is released in the cement production process. Meanwhile, the concrete is hard lining, so that building waste can be formed when a channel is changed in the future, and even soil can be polluted. Therefore, there is a need for a green and friendly technique for reinforcing the soil on the surface of the channel, so as to avoid the above problems.
The Microbial mineralization technology (MICP) is one of the most innovative technologies in geotechnical engineering. The technology creatively utilizes rich natural non-toxic microbial resources, and after a certain amount of harmless chemical reagents are added, the materials can be mineralized to generate calcium carbonate, so that the purposes of reinforcing the soil body and improving the engineering mechanical properties such as the strength, the impermeability and the scouring resistance of the soil body are achieved. The microorganism is a harmless bacterium naturally existing in the soil of the nature, and hardly has any influence on the soil, the human health and the environment, so the microorganism mineralization technology is an environment-friendly innovative technology.
At present, the microbial mineralization technology is still in a development stage, and generally only single indexes such as permeability, strength and the like of small soil samples are measured, so that the requirement of building ecological channels based on microbial mineralization cannot be met. Therefore, a special indoor test device needs to be developed, and the technology for building ecological channels based on microbial mineralization is developed by testing the soil strength, impermeability and scour resistance parameters before and after the microbial mineralization.
The invention content is as follows:
the invention aims to solve the problems, and designs a test device and a method for simulating the construction of an ecological channel based on microbial mineralization, which are used for researching materials and construction processes for constructing the ecological channel based on the microbial mineralization simulation.
In order to achieve the above object, the test apparatus and the test method according to the present invention are realized by the following means.
According to one aspect of the invention, a test device for simulating the construction of an ecological channel based on microbial mineralization is provided, which comprises a water supply system, an ecological channel test system and a measurement system which are connected in sequence. The water supply system is used for providing stable flow and simulating channel water flow; the ecological channel test system is used for simulating the running state of a channel under different microbial mineralization materials and construction processes; the measuring system is used for testing the strength, permeability and scour resistance of the soil body before and after the microbial mineralization treatment.
Specifically, the water supply system comprises a tap water pipe, a supporting steel frame, a high-level water tank, an overflow pipe, a water supply pipe, a flowmeter and a flow control valve. The supporting steel frame supports the high-level water tank at a certain height; the overflow pipe is positioned on the side wall of the top of the high-level water tank, so that the water level of the high-level water tank is constant; the water supply pipe is arranged on the side wall of the high-level water tank and is lower than the overflow pipe, and the other end of the water supply pipe extends into the energy dissipation pool, so that water in the high-level water tank can automatically flow into the energy dissipation pool by means of gravity; the flow control valve and the flow meter are arranged on the water supply pipe.
Specifically, ecological channel test system includes basin, soil sample case, energy dissipation pond, basin play sluice, adjusting support, hinged-support. The water tank is used for receiving water flow to form a channel model, and a layer of soil particles is adhered to the surface of the water tank and used for simulating the soil roughness condition under natural conditions; the soil sample box is positioned near the end part of the water tank, is a detachable trapezoidal groove with a porous plate at the bottom and is used for placing soil bodies of the simulation channel in the soil sample box; the energy dissipation pool is positioned at the other end of the water tank, is used for receiving the water flow of the high-level water tank and is used as a water storage container to provide water flow with a certain flow rate; the water tank water outlet gate is arranged at the end part of the water tank and used for regulating the water depth and the flow speed in the water tank together with the flow control valve; the adjusting bracket is positioned near the energy dissipation pool and can be used for adjusting the gradient of the water trough by being matched with the hinged support.
Specifically, the measuring system comprises a portable cross shear apparatus, a seepage flow measuring subsystem and a sediment flow measuring subsystem. The portable cross shear apparatus is used for testing the shear strength of a soil body; the seepage flow measuring subsystem comprises a seepage collecting cylinder and is placed right below the soil sample box; the silt quantity measuring subsystem comprises a silt collecting cylinder and is placed at the water flow outlet of the water tank.
According to a second aspect of the present invention, there is provided an indoor model test method for simulating the construction of an ecological channel based on microbial mineralization, comprising the following steps:
the first step is as follows: taking soil bodies of the base surface of the natural irrigation canal, and respectively placing the soil bodies in each soil sample box;
the second step is that: treating the soil body in each soil sample box by using different microbial mineralized materials and construction processes, and maintaining for a period of time until the surface of the soil body is hardened after the treatment is finished;
the third step: the device is used for testing the engineering mechanical property of the soil body of the channel, the soil sample box is arranged in the water tank, the adjusting bracket is adjusted to enable the water tank to be horizontal, the water outlet gate of the water tank is closed, the flow control valve is opened for supplying water, the water supply equipment is closed after the water level is immersed in the soil body of the irrigation canal model for a certain depth, and the device is stood for a period of time to enable the soil body to be fully saturated;
the fourth step: and (6) permeability testing. After the soil body is saturated for a period of time, opening a flow control valve to continue water supply, simultaneously keeping a water outlet gate of a water tank closed, filling the water tank with water flow, and measuring the water flow seepage quantity in a seepage collecting cylinder; then calculating the soil permeability coefficient according to the following formula:
in the formula, k is the soil permeability coefficient, m/s; q is the flow, m3S; h is the height of the water surface, m; l is the width of the bottom edge of the soil sample box, m; d is the length of the soil sample box, m; t is the soil thickness, m; alpha is the included angle between the side wall of the soil sample box and the horizontal plane (degree).
The fifth step: and (5) carrying out scour resistance test. Opening a water tank outlet gate to drain water in the water tank, adjusting the adjusting bracket to different gradients, then opening equipment in the water supply system, and adjusting the water depth and the flow rate in the water tank through a flow control valve and the water tank outlet gate; after scouring for a period of time, drying the silt collected in the silt collecting cylinder by adopting a drying method, and measuring the quality of the scoured silt; the soil separation capacity was then calculated according to the formula:
wherein D is the soil separating ability value, g/(m)2S); m1 is the soil wet weight, g; m2 is the soil moisture mass, g; m3 is the dry weight of the soil body at the end of the test, g; t is the flushing time, s; a is the channel surface area, m2。
And a sixth step: and (6) testing the strength. Testing the shear strength of the processed soil body by using a portable cross shear apparatus;
the seventh step: and (4) replacing the soil sample box, and repeating the third step to the sixth step on the soil body filled with different microbial mineralization materials and treated by the construction process.
Eighth step: determining the material and construction process for constructing the ecological irrigation canal based on microbial mineralization by comparing the water permeability, shear strength and impact resistance of the soil body treated by different parameters and processes.
Compared with the prior art, the invention has the following beneficial effects:
1. the device builds an indoor model of the channel, and can be used for researching the technology for building the ecological channel based on microbial mineralization by testing the soil strength, permeability and scour resistance parameters before and after different microbial mineralization materials and construction process treatment;
2. the flow control valve and the water tank outlet gate of the device can cooperatively regulate and control water depth and flow velocity, and the support is adjusted and the hinged support can be matched to adjust the gradient of the water tank, so that the indoor test can simulate the anti-scouring performance of soil bodies under multiple levels of water depth and flow velocity to meet the requirement of actual channel engineering simulation;
3. the soil sample box in the device can be disassembled, different soil bodies can be quickly replaced, and different microorganism mineralization materials and construction processes are tested, so that the test efficiency is improved, and the materials and the construction processes which are actually required by engineering are better determined.
Description of the drawings:
FIG. 1 is a schematic diagram of the apparatus of the present invention;
FIG. 2 is a cross-sectional view taken along line A-A of the apparatus of the present invention;
FIG. 3 is a cross-sectional view taken along line B-B of the apparatus of the present invention.
FIG. 4 is a schematic view of a seepage measurement subsystem of the apparatus of the present invention;
FIG. 5 is a schematic view of a silt measurement subsystem of the apparatus of the present invention;
FIG. 6 is a schematic diagram of the portable cross plate shear apparatus of the present invention measuring soil strength;
in the figure: 1 is high-order water tank, 2 is the running water pipe, 3 is the overflow pipe, 4 is the support steelframe, 5 is the flow control valve, 6 is the flowmeter, 7 is the delivery pipe, 8 is the energy dissipation pond, 9 is the basin, 10 is the soil sample case, 11 is the seepage flow collecting vessel, 12 is the silt collecting vessel, 13 is the regulation support, 14 is the hinged-support, 15 is the workstation, 16 is the basin water gate, 17 is soil sample case steel sheet, 18 is the soil sample, 19 is the basin steel sheet, 20 is the clay granule, 21 is portable cross plate shear apparatus.
The specific implementation mode is as follows:
the invention provides a test device for simulating construction of an ecological channel based on microbial mineralization. The water supply system is used for providing stable flow and simulating channel water flow; the ecological channel test system is used for simulating the running state of a channel under different microbial mineralization materials and construction processes; the measuring system is used for testing the strength, permeability and scour resistance of the soil body before and after the microbial mineralization treatment.
Specifically, the water supply system includes: tap water pipe 2, support steelframe 4, high-order water tank 1, overflow pipe 3, delivery pipe 7, flowmeter 6, flow control valve 5. The supporting steel frame 4 supports the high-level water tank 1 at a certain height; the overflow pipe 3 is positioned on the side wall of the top of the high-level water tank 1, so that the water level of the high-level water tank 1 is constant; the water supply pipe 7 is arranged on the side wall of the high-level water tank 1 and is lower than the overflow pipe 3, and the other end of the water supply pipe extends into the energy dissipation pool 8, so that water in the high-level water tank 1 can automatically flow into the energy dissipation pool 8 by means of gravity; the flow control valve 5 and the flow meter 6 are arranged on a water supply pipe 7.
Specifically, the ecological channel test system includes: the device comprises a water tank 9, a soil sample box 10, an energy dissipation pool 8, a water tank outlet sluice 16, an adjusting bracket 13 and a hinged support 14. The water tank 9 is used for receiving water flow to form a channel model, a layer of soil particles are adhered on the surface of the water tank, namely the adhered soil particles 20 are used for simulating the soil roughness condition under natural conditions; the soil sample box 10 is positioned near the end part of the water tank 9, is a detachable trapezoid groove with a porous plate on the bottom surface, and is used for placing a soil body of a simulation channel in the soil sample box 9; the energy dissipation pool 8 is positioned at the other end of the water tank 9, is used for receiving the water flow of the high-level water tank 1 and is used as a water storage container to provide water flow with a certain flow rate; the water tank outlet sluice 16 is arranged at the end part of the water tank 9 and is used for regulating the water depth and the flow rate in the water tank 9 together with the flow control valve 5; the adjusting bracket 13 is positioned near the energy dissipation pool 8 and can be used for adjusting the gradient of the water trough 9 by matching with the hinged support 14.
Specifically, the measurement system includes: a portable cross shear apparatus 21, a seepage flow measuring subsystem and a silt measuring subsystem. The portable cross shear apparatus 21 is used for testing the shear strength of the soil body; the seepage flow measuring subsystem comprises a seepage collecting cylinder 11 which is arranged right below the soil sample box 10; the silt quantity measuring subsystem comprises a silt collecting cylinder 12 which is arranged at the water flow outlet of the water tank 9.
According to a second aspect of the present invention, there is provided an indoor model test method for simulating the establishment of an ecological channel based on microbial mineralization, comprising the following steps:
the first step is as follows: soil bodies of the base surface of the natural irrigation canal are respectively placed in each soil sample box 10;
the second step is that: treating the soil body in each soil sample box 10 by using different microorganism mineralized materials and construction processes, and maintaining for a period of time after the treatment is finished until the surface of the soil body is hardened;
the third step: the device is used for testing the engineering mechanical property of the channel soil body, a soil sample box 10 is arranged in a water tank 9, an adjusting bracket 13 is adjusted to enable the water tank 9 to be horizontal, a water outlet gate 16 of the water tank is closed, a flow control valve 5 is opened for water supply, the flow control valve 5 is closed after the water level is immersed in the soil body of the irrigation canal model for a certain depth, and the device is stood for a period of time to enable the soil body to be fully saturated;
the fourth step: and (6) permeability testing. After the soil body is saturated for a period of time, opening the flow control valve 5 to continue water supply, simultaneously keeping the water tank outlet sluice 16 closed, filling the water tank 9 with water flow, and measuring the water flow seepage in the seepage collecting cylinder 11; then calculating the soil permeability coefficient according to the following formula:
in the formula, k is the soil permeability coefficient, m/s; q is the flow, m3S; h is the height of the water surface, m; l is the width of the bottom edge of the soil sample box, m; d is the length of the soil sample box, m; t is the soil thickness, m; alpha is the included angle between the side wall of the soil sample box and the horizontal plane (degree).
The fifth step: and (5) carrying out scour resistance test. Opening a water tank outlet sluice 16 to drain water in the water tank 9, adjusting the adjusting bracket 13 to different gradients, then opening equipment in the water supply system, and adjusting the water depth and flow rate in the water tank 9 through the flow control valve 5 and the water tank outlet sluice 16; after the silt is washed for a period of time, drying the silt collected in the silt collecting cylinder 12 by adopting a drying method, and measuring the quality of the washed silt; the soil separation capacity was then calculated according to the formula:
wherein D is the soil separating ability value, g/(m)2S); m1 is the soil wet weight, g; m2 is the soil moisture mass, g; m3 is the dry weight of the soil body at the end of the test, g; t is the flushing time, s; a is the channel surface area, m2。
And a sixth step: and (6) testing the strength. Testing the shear strength of the processed soil body by using a portable cross shear apparatus 21;
the seventh step: and (5) replacing the soil sample box 10, and repeating the third step to the sixth step on the soil body filled with different microorganism mineralization materials and treated by the construction process.
Eighth step: determining the material and construction process for constructing the ecological irrigation canal based on microbial mineralization by comparing the water permeability, shear strength and impact resistance of the soil body treated by different parameters and processes.
This embodiment is further described below in conjunction with the detailed description:
1. and (5) preparing a sample. Before loading, each soil sample box 10 is weighed net. And respectively loading samples in the plurality of soil sample boxes 10, wherein the samples are loaded according to the sample preparation requirements of 'geotechnical test regulations', and external air is prevented from entering the interior in the sample loading process. And sampling and measuring the soil moisture content.
2. And (4) carrying out microbial mineralization treatment. The microorganisms are cultivated by using a urease-producing Bacillus pasteurii strain, by culturing in a specific medium, culturing the Bacillus pasteurii strain in a nutrient medium at a cultivation temperature of 37 ℃ at a speed of 130 rpm, and subsequently obtaining a concentration of 5X 107cfu/mL (5X 10 per mL)7Viable bacteria count, i.e., optical density of 1.3), and the concentration of the Bacillus pasteurianus was determined by a diffusion plate method.
The specific preparation process of the culture medium comprises the following steps: dissolving 13.0g of a nutrient medium comprising 5.0g of peptone, 5.0g of sodium chloride (NaCl), 2.0g of yeast extract and 1.0g of meat extract in 1 liter of distilled water; then, autoclaving is carried out, and the pH value is made to be 7.0;
in the embodiment of the invention, the bacterial liquid, the calcium chloride solution and the urea solution can be independently sprayed and can be mixed with each other to form a mixed solution, so that different construction materials can be obtained. In the embodiment of the invention, one preferable scheme is that 0.05M of calcium chloride is mixed in the bacterial liquid to form a mixed solution A, and 0.5M of calcium chloride and 0.5M of urea are mixed to form a mixed solution B. And then, spraying soil on the surface of the channel by different construction processes.
One of the preferable construction processes is taken for detailed description: spraying the mixed solution A, spraying the mixed solution B after 6 hours, spraying the mixed solution A after 12 hours, and spraying the mixed solution A after 18 hoursSpraying the mixed solution B again, spraying 12 times at intervals in such a reciprocating manner, and curing for 7 days. To avoid clogging the surface and to allow the solution to penetrate deep and the precipitation to occur slowly, it is ensured that per m360kg of CaCO can be generated in the soil3Therefore, the treatment is carried out by adopting an interval spraying mode until the soil body on the surface is wet each time;
in the examples of the invention, CaCl was perfused2The purpose of the solution is to utilize Ca2+The flocculation of the microorganism realizes the fixation of the microorganism and simultaneously provides necessary product CaCO in a reaction system3The raw material of (1).
3. The permeability test carried out by the device of the invention has the following specific implementation modes: installing a soil sample box 10, adjusting an adjusting bracket 13 to enable a water tank to be horizontal, closing a water outlet gate 16 of the water tank, opening a flow control valve 5 and supplying water, closing the flow control valve 5 after the water level submerges the soil body of the irrigation ditch model to a certain depth, and standing for a period of time to enable the soil body to be fully saturated. After the soil body is saturated for a period of time, the flow control valve 5 is opened to continue water supply, meanwhile, the water tank outlet sluice 16 is kept closed, so that the water tank 9 is filled with water flow, and the water flow seepage quantity in the seepage collecting cylinder 11 is measured. The permeability coefficient was then calculated according to the following formula:
in the formula, k is the soil permeability coefficient, m/s; q is the flow, m3S; h is the height of the water surface, m; l is the width of the bottom edge of the soil sample box, m; d is the length of the soil sample box, m; t is the soil thickness, m; alpha is the included angle between the side wall of the soil sample box and the horizontal plane (degree).
The larger the calculated permeability coefficient is, the worse the permeability of the soil body is.
4. The specific implementation mode of the scour resistance test performed by the device provided by the invention is as follows: opening a water tank outlet sluice 16 to drain water in the water tank 9, adjusting the adjusting bracket 13 to different gradients, then opening equipment in the water supply system, and adjusting the water depth and flow rate in the water tank 9 through the flow control valve 5 and the water tank outlet sluice 16; after the sand is washed for a period of time, the sand collected in the sand collecting cylinder 12 is dried by a drying method, and the quality of the washed sand is measured. The soil separation capacity was then calculated according to the formula:
wherein D is the soil separating ability value, g/(m)2S); m1 is the soil wet weight, g; m2 is the soil moisture mass, g; m3 is the dry weight of the soil body at the end of the test, g; t is the flushing time, s; a is the channel surface area, m2。
The larger the calculated soil separation capacity value is, the poorer the impact resistance of the soil body is.
5. The strength test performed by the device of the invention has the following specific implementation mode: randomly taking 3 points on the surface of the channel as characteristic points, and then testing the shear strength of the soil body processed by the position of the characteristic points by using a portable cross shear apparatus 21; and then, carrying out statistical analysis on the measured shear strength value, and comparing the soil body strength of different microbial mineralization materials and the soil body strength treated by the construction process.
6. Replacing the soil sample box 10, namely replacing different test subsystems, and repeating the strength test, the permeability test and the scour resistance test of the device in the invention in the 3 rd, the 4 th and the 5 th steps until all the measurement subsystems are processed; the advantages and disadvantages of permeability and impact resistance among several test subsystems are compared with each other at this time, and thus, a preferred embodiment is selected as a preferred material and a preferred process treatment at the time of actual construction of the channel.
While specific embodiments of the present invention have been described above, it should be noted that: it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention, and any changes, substitutions, improvements and the like made within the spirit and principles of the invention should be considered within the scope of the invention.
Claims (2)
1. An indoor test device for simulating the construction of an ecological channel based on microbial mineralization is characterized by comprising a water supply system, an ecological channel test system and a measurement system which are sequentially connected; the water supply system is used for providing stable flow and simulating channel water flow; the ecological channel test system is used for simulating the running state of a channel under different microbial mineralization materials and construction processes; the measuring system is used for testing the permeability, the scour resistance and the strength of the soil body before and after the microbial mineralization treatment;
the ecological channel test system comprises a water tank, a soil sample box, an energy dissipation pool, a water tank water outlet gate, an adjusting bracket and a hinged support; the water tank is used for receiving water flow to form a channel model, and a layer of soil particles is adhered to the surface of the water tank and used for simulating the soil roughness condition under natural conditions; the soil sample box is positioned near the end part of the water tank, is a detachable trapezoidal groove with a porous plate at the bottom and is used for placing soil bodies of the simulation channel in the soil sample box; the energy dissipation pool is positioned at the other end of the water tank, is used for receiving the water flow of the high-level water tank and is used as a water storage container to provide water flow with a certain flow rate; the water tank water outlet gate is arranged at the end part of the water tank and used for regulating the water depth and the flow speed in the water tank together with the flow control valve; the adjusting bracket is positioned near the energy dissipation pool and can be matched with the hinged support to adjust the gradient of the water trough;
the water supply system comprises a tap water pipe, a supporting steel frame, a high-level water tank, an overflow pipe, a water supply pipe, a flowmeter and a flow control valve; the supporting steel frame supports the high-level water tank at a certain height; the overflow pipe is positioned on the side wall of the top of the high-level water tank, so that the water level of the high-level water tank is constant; the water supply pipe is arranged on the side wall of the high-level water tank and is lower than the overflow pipe, and the other end of the water supply pipe extends into the energy dissipation pool, so that water in the high-level water tank can automatically flow into the energy dissipation pool by means of gravity; the flow control valve and the flowmeter are arranged on the water supply pipe;
the measuring system comprises a portable cross shear apparatus, a seepage flow measuring subsystem and a sediment flow measuring subsystem; the portable cross shear apparatus is used for testing the shear strength of a soil body; the seepage flow measuring subsystem comprises a seepage collecting cylinder and is placed right below the soil sample box; the silt quantity measuring subsystem comprises a silt collecting cylinder and is placed at the water flow outlet of the water tank.
2. The test method for simulating the indoor test device for building the ecological channel based on the microbial mineralization according to claim 1, which is characterized by comprising the following steps:
the first step is as follows: taking soil bodies of the channel base surface, and respectively placing the soil bodies in each soil sample box;
the second step is that: treating the soil body in each soil sample box by using different microbial mineralized materials and construction processes, and maintaining for a period of time until the surface of the soil body is hardened after the treatment is finished;
the third step: installing a soil sample box in a water tank, adjusting an adjusting bracket to enable the water tank to be horizontal, closing a water outlet gate of the water tank, opening a flow control valve to supply water, closing water supply equipment after the water level is immersed in a channel model soil body for a certain depth, and standing for a period of time to enable the soil body to be fully saturated;
the fourth step: performing permeability test, after the soil body is saturated for a period of time, opening the flow control valve to continue water supply, simultaneously keeping the water outlet gate of the water tank closed, filling the water tank with water flow, and measuring the water flow seepage quantity in the seepage collecting cylinder; then calculating the soil permeability coefficient according to the following formula:
in the formula, k is the soil permeability coefficient, m/s; q is the flow, m3S; h is the height of the water surface, m; l is the width of the bottom edge of the soil sample box, m; d is the length of the soil sample box, m; t is the soil thickness, m; alpha is the included angle between the side wall of the soil sample box and the horizontal plane;
the fifth step: the anti-scouring test comprises the steps of opening a water tank outlet gate to drain water in the water tank, adjusting an adjusting bracket to different slopes, then opening equipment in a water supply system, and adjusting the water depth and the flow rate in the water tank through a flow control valve and the water tank outlet gate; after scouring for a period of time, drying the silt collected in the silt collecting cylinder by adopting a drying method, and measuring the quality of the scoured silt; the soil separation capacity was then calculated according to the formula:
wherein D is the soil separating ability value, g/(m)2S); m1 is the soil wet weight, g; m2 is the soil moisture mass, g; m3 is the dry weight of the soil body at the end of the test, g; t is the flushing time, s; a is the channel surface area, m2；
And a sixth step: testing the strength, namely testing the shear strength of the processed soil body by using a portable cross shear apparatus;
the seventh step: replacing the soil sample box, and repeating the third step to the sixth step on the soil body filled with different microbial mineralization materials and treated by the construction process;
eighth step: and determining the material for building the ecological channel based on the microbial mineralization and the construction process by comparing the water permeability, the shear strength and the impact resistance of the soil body after different parameters and process treatment.
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