CN111154644B - Polymerase chain reaction amplification full-automatic workstation - Google Patents
Polymerase chain reaction amplification full-automatic workstation Download PDFInfo
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- CN111154644B CN111154644B CN202010055308.1A CN202010055308A CN111154644B CN 111154644 B CN111154644 B CN 111154644B CN 202010055308 A CN202010055308 A CN 202010055308A CN 111154644 B CN111154644 B CN 111154644B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0848—Specific forms of parts of containers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1894—Cooling means; Cryo cooling
Abstract
The invention discloses a polymerase chain reaction amplification full-automatic workstation, which comprises a mixing box, wherein a mixing device extending into the mixing box is arranged on the mixing box, the bottom of the mixing box is connected with a plurality of reaction boxes through a feeding pipeline, a discharging mechanism extending into the reaction boxes is arranged on the reaction boxes, a main body temperature control device is arranged on the outer side of each reaction box, and the bottom of each reaction box is connected with a low-temperature storage box through a discharging pipeline. The invention has the advantages of rapid temperature regulation and thorough blanking.
Description
Technical Field
The invention relates to the field of biomedicine and genetic genes, in particular to a polymerase chain reaction amplification full-automatic workstation.
Background
In the PCR (polymerase chain reaction), DNA is denatured at 95 ℃ in vitro to become single-stranded, and at low temperature (usually about 60 ℃) the primer and single-stranded are combined according to the principle of base complementary pairing, and then the temperature is adjusted to the optimum reaction temperature (about 72 ℃) of DNA polymerase, and the DNA polymerase synthesizes a complementary strand along the direction from phosphate to pentose (5 '-3'). The PCR instrument manufactured based on polymerase is actually a temperature control device, and can be well controlled among denaturation temperature, renaturation temperature and extension temperature.
The existing polymerase chain reaction device is not rapid enough for adjusting the temperature in a reaction vessel, and the product concentration in the reaction vessel is large after the reaction is finished, and the blanking is not thorough enough, so that aiming at the current situation, the development of a polymerase chain reaction amplification full-automatic workstation with rapid temperature adjustment and thorough blanking is urgently needed to overcome the defects in the current practical application and meet the current requirements.
Disclosure of Invention
The present invention is directed to a fully automatic workstation for PCR amplification, which solves the above problems.
In order to achieve the purpose, the invention provides the following technical scheme:
the utility model provides a full-automatic workstation of polymerase chain reaction amplification, includes the mixing box, install on the mixing box and extend to its inside mixing arrangement, the bottom of mixing box passes through charge-in pipeline and links to each other with a plurality of reaction boxs, install on the reaction box and extend to its inside unloading mechanism, main part temperature regulating device is installed in the outside of reaction box, the bottom of reaction box is passed through unloading pipeline and is linked to each other with the low temperature storage box.
As a further scheme of the invention: the unloading mechanism includes: second motor, screw rod, nut, scrape glassware, spring, synchronizing wheel and hold-in range, the inside left and right sides of reaction box installs respectively one and extends to its outside screw rod, two all install a nut on the screw rod, the downside of nut is installed in the reaction box rather than the glassware of scraping of laminating mutually, it links to each other with the top of reaction box through a plurality of springs to scrape the glassware, install the second motor that links to each other with the screw rod on the reaction box, two all install a synchronizing wheel, two on the screw rod the synchronizing wheel passes through the hold-in range and rotates the connection.
As a further scheme of the invention: the mixing device comprises: the mixing box comprises a first motor, a coupler and a mixer, wherein an output shaft of the first motor extends into the mixing box, and the output shaft of the first motor is connected with the mixer through the coupler.
As a further scheme of the invention: the main body temperature control device comprises: the hot water tank, the annular winding pipe, the cold water tank, hot water pipe, cold water pipe, first back flow pipe and second back flow pipe, the annular winding pipe is installed in the outside of reaction box, the left end of annular winding pipe passes through the hot water pipe and links to each other with the hot water tank, the left end of annular winding pipe passes through the cold water pipe and links to each other with the cold water tank, the right-hand member of annular winding pipe passes through first back flow and links to each other with the hot water tank, the right-hand member of annular winding pipe passes through the second back flow and links to each other with the cold water tank.
As a further scheme of the invention: and the hot water pipe and the first return pipe are both provided with a first electromagnetic valve.
As a further scheme of the invention: and the cold water pipe and the second return pipe are both provided with a second electromagnetic valve.
As a further scheme of the invention: the storage temperature of the low-temperature storage box is 3-5 ℃.
Compared with the prior art, the invention has the beneficial effects that: the full-automatic polymerase chain reaction amplification workstation has the advantages that DNA and a catalyst are added into a mixing box, the DNA and the catalyst are fully mixed through a mixing device, the mixed DNA and the mixed catalyst are added into a reaction box, when two first electromagnetic valves are opened, hot water is supplied to an annular winding pipe through a hot water tank, the reaction box is rapidly heated through the annular winding pipe and an auxiliary heating device, when two second electromagnetic valves are opened, cold water is supplied to the annular winding pipe through a cold water tank, the auxiliary heating device is closed, the reaction box is cooled through the annular winding pipe, and the temperature in the reaction box can be rapidly adjusted; after the reaction is completed, the screw rod is driven to rotate through the second motor, the nut is driven to move downwards through the rotation of the screw rod, the material scraping device is driven to move downwards through the downward movement of the nut, and products on the inner wall of the reaction box are scraped through the material scraping device, so that the discharging is more thorough. In conclusion, the invention has the advantages of rapid temperature regulation and thorough blanking.
Drawings
Fig. 1 is a front sectional view of the present invention.
Fig. 2 isbase:Sub>A view frombase:Sub>A-base:Sub>A in front cross section of the present invention.
In the figure: 1-mixing box, 2-mixing device, 201-first motor, 202-coupler, 203-mixer, 3-feeding pipeline, 4-reaction box, 5-blanking mechanism, 501-second motor, 502-screw, 503-nut, 504-scraper, 505-spring, 506-synchronizing wheel, 507-synchronous belt, 6-auxiliary heating device, 7-main body temperature control device, 701-hot water box, 702-annular winding pipe, 703-cold water box, 704-hot water pipe, 705-cold water pipe, 706-first return pipe, 707-second return pipe, 8-first electromagnetic valve, 9-second electromagnetic valve, 10-blanking pipeline and 11-low-temperature storage box.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1
Referring to fig. 1-2, in an embodiment of the present invention, a full-automatic pcr amplification workstation includes a mixing box 1, a mixing device 2 extending into the mixing box 1 is installed on the mixing box 1, and the mixing device 2 includes: first motor 201, shaft coupling 202 and blender 203, the output shaft of first motor 201 extends to in the mixing box 1, the output shaft of first motor 201 passes through shaft coupling 202 and links to each other with blender 203, the bottom of mixing box 1 passes through charge-in pipeline 3 and links to each other with a plurality of reaction boxes 4, install on the reaction box 4 and extend to its inside unloading mechanism 5, main part temperature regulating device 7 is installed in the outside of reaction box 4, main part temperature regulating device 7 includes: the hot water tank 701, the annular winding pipe 702, the cold water tank 703, the hot water pipe 704, the cold water pipe 705, the first return pipe 706 and the second return pipe 707, the annular winding pipe 702 is installed on the outer side of the reaction tank 4, the left end of the annular winding pipe 702 is connected with the hot water tank 701 through the hot water pipe 704, the left end of the annular winding pipe 702 is connected with the cold water tank 703 through the cold water pipe 705, the right end of the annular winding pipe 702 is connected with the hot water tank 701 through the first return pipe 706, the right end of the annular winding pipe 702 is connected with the cold water tank 703 through the second return pipe 707, the hot water pipe 704 and the first return pipe 706 are both provided with a first electromagnetic valve 8, the cold water pipe 705 and the second return pipe 707 are both provided with a second electromagnetic valve 9, the left side and the right side of the bottom of the reaction tank 4 are respectively provided with an auxiliary heating device 6, the bottom of the reaction tank 4 is connected with the low-temperature storage tank 11 through a blanking pipeline 10, and the storage temperature of the low temperature storage tank 11 is 3-5 ℃.
Example 2
Referring to fig. 1-2, in the embodiment of the present invention, the blanking mechanism 5 includes: second motor 501, screw rod 502, nut 503, scrape glassware 504, spring 505, synchronizing wheel 506 and hold-in range 507, the inside left and right sides of reaction box 4 installs one respectively and extends to its outside screw rod 502, two all install a nut 503 on the screw rod 502, install rather than the glassware 504 of scraping of laminating mutually in reaction box 4 the downside of nut 503, it links to each other with the top of reaction box 4 through a plurality of springs 505 to scrape glassware 504, install the second motor 501 that links to each other with screw rod 502 on the reaction box 4, two all install a synchronizing wheel 506 on the screw rod 502, two synchronizing wheel 506 passes through hold-in range 507 and rotates the connection.
According to the full-automatic workstation for polymerase chain reaction amplification, DNA and a catalyst are added into a mixing box 1, the DNA and the catalyst are fully mixed through a mixing device 2, the mixed DNA and the catalyst are added into a reaction box 4, when two first electromagnetic valves 8 are opened, hot water is supplied to an annular winding pipe 702 through a hot water tank 701, the reaction box 4 is rapidly heated through the annular winding pipe 702 and an auxiliary heating device 6, when two second electromagnetic valves 9 are opened, cold water is supplied to the annular winding pipe 702 through a cold water tank 703, the auxiliary heating device 6 is closed, the reaction box 4 is cooled through the annular winding pipe 702, and the temperature in the reaction box 4 can be rapidly adjusted; after the reaction is finished, the screw 502 is driven to rotate through the second motor 501, the nut 503 is driven to move downwards through the rotation of the screw 502, the scraper 504 is driven to move downwards through the downward movement of the nut 503, and products on the inner wall of the reaction box 4 are scraped through the scraper 504, so that more thorough blanking is facilitated.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (6)
1. The full-automatic polymerase chain reaction amplification workstation comprises a mixing box (1) and is characterized in that a mixing device (2) extending into the mixing box (1) is installed on the mixing box (1), the bottom of the mixing box (1) is connected with a plurality of reaction boxes (4) through a feeding pipeline (3), a blanking mechanism (5) extending into the reaction boxes (4) is installed on the reaction boxes (4), a main body temperature control device (7) is installed on the outer side of each reaction box (4), and the bottom of each reaction box (4) is connected with a low-temperature storage box (11) through a blanking pipeline (10);
the blanking mechanism (5) comprises: second motor (501), screw rod (502), nut (503), scrape glassware (504), spring (505), synchronizing wheel (506) and hold-in range (507), one screw rod (502) that extends to its outside is installed respectively to the inside left and right sides of reaction box (4), two all install one nut (503) on screw rod (502), install in reaction box (4) the downside of nut (503) rather than scraping glassware (504) of laminating mutually, it links to each other with the top of reaction box (4) through a plurality of springs (505) to scrape glassware (504), install second motor (501) that link to each other with screw rod (502) on reaction box (4), two all install a synchronizing wheel (506) on screw rod (502), two synchronizing wheel (506) rotate through hold-in range (507) and connect.
2. The PCR amplification fully automated workstation according to claim 1, wherein the mixing device (2) comprises: the mixing box comprises a first motor (201), a coupler (202) and a mixer (203), wherein an output shaft of the first motor (201) extends into the mixing box (1), and the output shaft of the first motor (201) is connected with the mixer (203) through the coupler (202).
3. The PCR amplification fully automated workstation according to claim 1, wherein the main body temperature control device (7) comprises: the reactor comprises a hot water tank (701), an annular winding pipe (702), a cold water tank (703), a hot water pipe (704), a cold water pipe (705), a first return pipe (706) and a second return pipe (707), wherein the annular winding pipe (702) is installed on the outer side of a reaction tank (4), the left end of the annular winding pipe (702) is connected with the hot water tank (701) through the hot water pipe (704), the left end of the annular winding pipe (702) is connected with the cold water tank (703) through the cold water pipe (705), the right end of the annular winding pipe (702) is connected with the hot water tank (701) through the first return pipe (706), and the right end of the annular winding pipe (702) is connected with the cold water tank (703) through the second return pipe (707).
4. The PCR amplification fully automated workstation according to claim 3, wherein a first solenoid valve (8) is installed on each of the hot water pipe (704) and the first return pipe (706).
5. The PCR amplification fully automated workstation of claim 3, wherein a second solenoid valve (9) is installed on each of the cold water pipe (705) and the second return pipe (707).
6. The PCR amplification fully automated workstation according to claim 1, wherein the storage temperature of the low temperature storage tank (11) is 3-5 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010055308.1A CN111154644B (en) | 2020-01-17 | 2020-01-17 | Polymerase chain reaction amplification full-automatic workstation |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010055308.1A CN111154644B (en) | 2020-01-17 | 2020-01-17 | Polymerase chain reaction amplification full-automatic workstation |
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| CN111154644A CN111154644A (en) | 2020-05-15 |
| CN111154644B true CN111154644B (en) | 2023-02-10 |
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Citations (7)
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| CN101522909A (en) * | 2006-05-17 | 2009-09-02 | 加利福尼亚技术学院 | Thermal cycling system |
| CN101589157A (en) * | 2006-10-06 | 2009-11-25 | 万达利亚研究公司 | The method that is used for continuous rapid thermal cycles system |
| CN102046291A (en) * | 2008-04-04 | 2011-05-04 | It-Is国际有限公司 | Thermal control system and method for chemical and biochemical reactions |
| CN201942671U (en) * | 2010-11-30 | 2011-08-24 | 北京金菩嘉医疗科技有限公司 | Fully automatic working station for polymerase chain reaction amplification |
| CN104893951A (en) * | 2014-03-03 | 2015-09-09 | 北京双诚联盈净化工程技术有限公司 | Combination of polymerase chain reaction workstation |
| CN206529481U (en) * | 2017-03-03 | 2017-09-29 | 河南普乐泰生物科技股份有限公司 | It is a kind of can enzyme digestion reaction vacuum scraper concentrator |
| CN208166986U (en) * | 2018-03-29 | 2018-11-30 | 佛山市南海区宏冠畜牧水产饲料有限公司 | The intelligent fermentor of one breeding hen feed production |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7622296B2 (en) * | 2004-05-28 | 2009-11-24 | Wafergen, Inc. | Apparatus and method for multiplex analysis |
| JP2015188378A (en) * | 2014-03-28 | 2015-11-02 | セイコーエプソン株式会社 | Nucleic acid analysis apparatus and nucleic acid analysis method |
-
2020
- 2020-01-17 CN CN202010055308.1A patent/CN111154644B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101522909A (en) * | 2006-05-17 | 2009-09-02 | 加利福尼亚技术学院 | Thermal cycling system |
| CN101589157A (en) * | 2006-10-06 | 2009-11-25 | 万达利亚研究公司 | The method that is used for continuous rapid thermal cycles system |
| CN102046291A (en) * | 2008-04-04 | 2011-05-04 | It-Is国际有限公司 | Thermal control system and method for chemical and biochemical reactions |
| CN201942671U (en) * | 2010-11-30 | 2011-08-24 | 北京金菩嘉医疗科技有限公司 | Fully automatic working station for polymerase chain reaction amplification |
| CN104893951A (en) * | 2014-03-03 | 2015-09-09 | 北京双诚联盈净化工程技术有限公司 | Combination of polymerase chain reaction workstation |
| CN206529481U (en) * | 2017-03-03 | 2017-09-29 | 河南普乐泰生物科技股份有限公司 | It is a kind of can enzyme digestion reaction vacuum scraper concentrator |
| CN208166986U (en) * | 2018-03-29 | 2018-11-30 | 佛山市南海区宏冠畜牧水产饲料有限公司 | The intelligent fermentor of one breeding hen feed production |
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| CN111154644A (en) | 2020-05-15 |
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