CN111141569B - Hydrops class pathological analysis sediment layer extraction element - Google Patents
Hydrops class pathological analysis sediment layer extraction element Download PDFInfo
- Publication number
- CN111141569B CN111141569B CN202010074968.4A CN202010074968A CN111141569B CN 111141569 B CN111141569 B CN 111141569B CN 202010074968 A CN202010074968 A CN 202010074968A CN 111141569 B CN111141569 B CN 111141569B
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- piston
- shell
- test tube
- disc
- pathological analysis
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- 238000010827 pathological analysis Methods 0.000 title claims abstract description 14
- 238000000605 extraction Methods 0.000 title claims abstract description 7
- 239000013049 sediment Substances 0.000 title claims description 11
- 241000521257 Hydrops Species 0.000 title description 2
- 206010030113 Oedema Diseases 0.000 title description 2
- 239000007788 liquid Substances 0.000 claims abstract description 18
- 230000000149 penetrating effect Effects 0.000 claims abstract description 5
- 230000008021 deposition Effects 0.000 claims abstract description 4
- 239000002244 precipitate Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 2
- 239000004033 plastic Substances 0.000 claims description 2
- 229920003023 plastic Polymers 0.000 claims description 2
- 239000012530 fluid Substances 0.000 claims 4
- 238000009825 accumulation Methods 0.000 claims 2
- 230000007170 pathology Effects 0.000 claims 1
- 238000004804 winding Methods 0.000 claims 1
- 239000006228 supernatant Substances 0.000 abstract description 20
- 238000000034 method Methods 0.000 abstract description 4
- 238000005119 centrifugation Methods 0.000 description 8
- 239000010902 straw Substances 0.000 description 6
- 208000002151 Pleural effusion Diseases 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 208000026216 Thoracic disease Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 230000001613 neoplastic effect Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 239000012945 sealing adhesive Substances 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000005204 segregation Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
Abstract
The invention relates to a deposition layer extraction device for pathological analysis of effusion, which effectively solves the problem that supernatant cannot be directly extracted in the centrifugal process; the technical scheme for solving the problem is that the device comprises a box body, wherein a cover is arranged on the box body, and a rotatable disc is arranged in the box body; a plurality of buckles are evenly distributed on the circumference of the disc, test tubes are arranged on the buckles, a cylindrical shell can be detachably mounted at an opening of each test tube, a piston is mounted in the shell, a plurality of piston rods penetrating through the shell are fixed on the inner side of the piston, a hard suction tube is mounted on the piston, the outer end of the suction tube penetrates through the shell and is placed in the test tube, and the inner end of the suction tube is fixed with the piston and extends out of the inner side of the piston; an air inlet pipe with one end arranged in the test tube and the other end arranged outside the shell penetrates through the shell, and a one-way air inlet valve is arranged on the air inlet pipe; the outer edge surface of the shell is provided with an exhaust port, and the suction pipe is provided with a one-way liquid inlet valve; a guide rod penetrates through the cover, and a conical disc is fixed at the lower end of the guide rod; the invention has the advantages of convenient supernatant extraction and improved working efficiency.
Description
Technical Field
The invention relates to the field of pathological analysis sample treatment, in particular to a device for extracting a deposition layer for pathological analysis of effusion.
Background
The pleural effusion is a common accompanying sign of various thoracic diseases, particularly neoplastic diseases, even appears for the first onset of patients, and the clear property of the pleural effusion has important significance for the diagnosis and treatment of the diseases; centrifugation, smear and staining are generally performed in general pathological examination, namely after pleural effusion is centrifuged, frozen sections of a sediment layer are subjected to conventional H staining, and the method has high repeatability, clear cells and clear diagnosis. Although the centrifuge that present hospital was equipped with can be fast carries out centrifugal treatment to the sample in the test tube, the sample after the centrifugation generally only needs to remain the precipitate layer, so just need after the centrifugation finishes, through long-time stationary precipitation after, separates, has prolonged the time of preparation sample.
At present, some centrifugal devices can directly pump away supernatant after centrifugation, but various problems exist, 1, common test tubes cannot be used, and special containers are needed, so that the cost is increased; 2. the sedimentation layer and the supernatant are easy to be mixed together instead of being extracted under the condition of high-speed rotation; 3. because the proportion of the supernatant is different due to different samples, the extraction 4 is needed according to the situation, and the common test tube is inclined or vertically arranged, so that the precipitation effect is poor, and the centrifugal force is not strong.
In summary, there is a need for a centrifuge device that effectively addresses the above-mentioned problems.
Disclosure of Invention
In view of the above situation, the present invention provides a device for extracting a sediment layer in an accumulated liquid pathological analysis, which can effectively solve the problem that the supernatant cannot be directly extracted in the centrifugation process.
The technical scheme for solving the problem is that the device comprises a box body, wherein a detachable cover is arranged at the upper end of the box body, and a rotatable disc is arranged at the center of the bottom of the box body; a plurality of buckles are uniformly distributed on the circumference of the disc, each buckle is fixedly provided with a test tube, the opening of each test tube faces inwards, a cylindrical shell can be detachably mounted at the opening of each test tube, the inner diameter of the shell is the same as that of each test tube, a piston is mounted in the shell, a plurality of piston rods penetrating through the shell are fixed on the inner side of each piston, and the piston rods can drive the pistons to reciprocate and reset inwards;
the piston is provided with a hard suction tube, the outer end of the suction tube penetrates through the shell and is arranged in the test tube, and the inner end of the suction tube is fixed with the piston and extends out of the inner side of the piston; an air inlet pipe with one end arranged in the test tube and the other end arranged outside the shell penetrates through the shell, and a one-way air inlet valve is arranged on the air inlet pipe; the outer edge surface of the shell is provided with an exhaust port, and the suction pipe is provided with a one-way liquid inlet valve;
the cap is penetrated with a vertical guide rod, the lower end of the guide rod is fixed with a conical disc with the small end facing downwards, and the outer edge surface of the conical disc can extrude the piston rod by pressing the conical disc, so that the piston drives the suction pipe to move outwards simultaneously.
The invention can directly separate the supernatant after the centrifugal separation of the accumulated liquid, does not need special equipment, is simple and quick, is convenient to extract and improves the working efficiency.
Drawings
Fig. 1 is a front view of the present invention.
FIG. 2 is a front sectional view of the present invention.
FIG. 3 is a top sectional view of the present invention.
Fig. 4 is a front sectional view of the test tube, the housing, the pipette, the piston rod, and the like.
Fig. 5 is a state diagram of fig. 4.
Fig. 6 is a perspective view of the matching relationship of the conical disc, the guide rod and the adjusting nut.
FIG. 7 is a perspective view of the fit relationship between the pipette, piston, and piston rod.
Detailed Description
The following describes in further detail embodiments of the present invention with reference to the accompanying drawings.
As shown in fig. 1 to 7, the invention comprises a box body 1, a detachable cover 2 is arranged at the upper end of the box body 1, and a rotatable disc 3 is arranged at the center of the bottom of the box body 1; a plurality of buckles 4 are uniformly distributed on the circumference of the disc 3, each buckle 4 is fixedly provided with a test tube 5, the opening of each test tube 5 faces inwards, a cylindrical shell 6 can be detachably mounted at the opening of each test tube 5, the inner diameter of each shell 6 is the same as that of each test tube 5, a piston 7 is mounted in each shell 6, a plurality of piston rods 8 penetrating through each shell 6 are fixed on the inner side of each piston 7, and each piston rod 8 can drive each piston 7 to reciprocate and reset inwards;
a hard suction pipe 9 is arranged on the piston 7, the outer end of the suction pipe 9 penetrates through the shell 6 and is arranged in the test tube 5, and the inner end of the suction pipe 9 is fixed with the piston 7 and extends out of the inner side of the piston 7; an air inlet pipe 10 with one end arranged inside the test tube 5 and the other end arranged outside the shell 6 penetrates through the shell 6, and a one-way air inlet valve 11 is arranged on the air inlet pipe 10; an exhaust port 12 is arranged on the outer edge surface of the shell 6, and a one-way liquid inlet valve 13 is arranged on the suction pipe 9;
a vertical guide rod 14 penetrates through the cover 2, a conical disc 15 with a downward small end is fixed at the lower end of the guide rod 14, the conical disc 15 is pressed, the outer edge surface of the conical disc 15 can extrude the piston rod 8, and the piston 7 drives the suction pipe 9 to move outwards at the same time.
In order to facilitate the liquid discharge, the inner end of the shell 6 is provided with a liquid discharge valve 16 through a pipeline.
In order to achieve the pumping of the supernatant, the air inlet valve 11 only allows the gas to enter the inside of the test tube 5, and the liquid inlet valve 13 only allows the liquid in the test tube 5 to enter the housing 6.
In order to realize the rotation of the disc 3, a motor 17 is installed at the bottom of the shell 6, and an output shaft of the motor 17 is fixedly connected with the center of the lower end face of the disc 3.
In order to facilitate connection and guarantee the sealing of the test tube 5 and the shell 6, the test tube 5 is made of plastic, the test tube 5 is connected with the shell 6 through threads, and a sealing adhesive tape is wound on the test tube 5.
In order to realize sealing, the joints of the suction pipe 9 and the shell 6 and the joints of the piston rod 8 and the shell 6 are sealed by rubber rings.
For better fixing of the test tubes 5, each of said housings 6 is also fitted with a catch 4.
In order to realize fixation, the buckle 4 comprises a vertical rod 18 fixed on the disc 3, and an anchor ear 19 is fixedly arranged at the upper end of the vertical rod 18.
In order to realize the detachment of the cover 2, a plurality of bolts capable of penetrating through the cover 2 are installed on the upper end surface of the box body 1, and nuts are screwed on the bolts.
In order to control the movement and fixation of the guide rod 14, a permanent magnet 20 capable of adsorbing the conical disc 15 is arranged on the cover 2; the guide rod 14 is provided with scales, and the outer edge surface of the guide rod 14 is in threaded connection with an adjusting nut 21.
In order to facilitate the conical disc 15 to extrude the piston rods 8, the number of the piston rods 8 on each piston 7 is two, the inner end of each piston rod 8 is provided with a ball 22, and each piston rod 8 is sleeved with a pressure spring 24 positioned between the ball 22 and the shell 6; the two piston rods 8 are connected together via a connecting rod 23 for added strength.
When the invention is used, the test tube 5 is vertically placed, and the effusion which needs to be centrifuged is put into the test tube 5, and the amount of the effusion is preferably that when the shell 6 is fixed on the test tube 5, the effusion just covers the end part of the suction tube 9. Then, the test tube 5 and the shell 6 need to be fixed on the hoop 19 of the disc 3 at the same time; the fixed positions of the test tube 5 and the shell 6 are preferably that the inner end of the piston rod 8 is just contacted with the conical disc 15 under the unstressed state; and the mark pen is used for marking the positions of the shell 6 and the buckle 4, so that the mark pen is convenient for subsequent use.
After each combination of test tube 5 and housing 6 is fixed in sequence in the manner described above, the centrifugation operation can be performed.
During centrifugation, only need open motor 17, alright in order to carry out high-speed centrifugal operation, at centrifugal in-process, should guarantee that conical disk 15 adsorbs on permanent magnet 20 all the time, avoids conical disk 15 extrusion piston rod 8.
Because test tube 5 level is placed in this device, consequently can be great improvement test tube 5's centrifugal force, compare and arrange or vertical arranging in traditional slope, can accelerate the centrifugation.
After centrifuging for a certain time, sucking away the supernatant at the moment; the specific operation is that firstly, the proportion of the supernatant to the sediment layer is determined according to relevant records or experimental experiences; and the distance of the guide rod 14 needing to be pressed is calculated according to the inclination angle of the conical disc 15, then the adjusting nut 21 is screwed according to the scale on the guide rod 14, the adjusting nut 21 is used for positioning, and the guide rod 14 descends.
Then, the conical disc 15 can be pressed down stably, and when the conical disc 15 descends, the conical disc 15 can extrude the piston rod 8, so that the piston rod 8 drives the piston 7 to move outwards; when the piston rod 8 moves outwards, a negative pressure chamber is formed between the inner side surface of the piston 7 and the inner wall of the shell 6; while the gas between the outer side of the piston 7 and the side wall of the housing 6 will be expelled through the exhaust port 12.
Simultaneously because straw 9 and piston 7 fixed connection to the inner of straw 9 stretches out piston 7's medial surface a short distance, consequently under the effect of the negative pressure between piston 7 medial surface and the 6 inner walls of casing, straw 9 can inhale the supernatant in the test tube 5 in the cavity between piston 7 right side and the 6 inner walls of casing, and test tube 5 is inside can inhale through intake pipe 10 this moment, has guaranteed to maintain atmospheric pressure in the test tube 5.
Because piston 7 and straw 9 fixed connection, and the casing 6 internal diameter is the same with the internal diameter of test tube 5, consequently along with the removal of piston 7, straw 9 also can outwards remove in step, has guaranteed that the tip of straw 9 can be arranged in the supernatant always to realize stable absorption supernatant.
When the guide rod 14 is not descending, the piston rod 8 also stops moving, the conical disc 15 can be pulled upwards, and the conical disc 15 is adsorbed on the permanent magnet 20 again; when the conical disc 15 rises, the piston rod 8 is not pushed at this time, and the piston 7 has a tendency of returning inwards under the action of the compression spring 24 on the piston rod 8, but the supernatant between the inner side surface of the piston 7 and the inner side wall of the shell 6 cannot be discharged because the suction pipe 9 is provided with the one-way valve; thereby making the piston 7 immovable and ensuring that the supernatant is always inside the housing 6.
Then the motor 17 can be stopped, the test tube 5 and the shell 6 are taken out, and the sediment in the shell 6 is subjected to the next wind treatment; the supernatant liquid in the shell 6 needs to be poured out through a drain valve 16; the shell 6 is fully cleaned and disinfected, so that the next use is facilitated; disposable materials may also be used to ensure sample accuracy.
According to the device, the shell 6 is innovatively arranged at the opening of the test tube 5, the piston 7, the suction tube 9 and the like are ingeniously designed, so that separated supernatant in the test tube 5 can be easily transferred into the shell 6, and rapid and accurate separation is realized; only precipitates are left in the test tube 5, so that the next treatment such as frozen section and the like can be conveniently and rapidly carried out; the working efficiency is improved.
The 5 levels of test tube are placed in this device, can improve centrifugal force, and the centrifugal process accelerates, and simultaneously under high-speed centrifugal's state, directly separate, guaranteed that supernatant and precipitate layer are in the state of alternate segregation always, have guaranteed the accuracy of proposing.
In the device, the purpose of moving the plurality of piston rods 8 is realized by pressing the guide rod 14, so that the device is convenient, simple and accurate to operate; can once only aspirate the supernatant in a plurality of test tubes 5 in step, great improvement efficiency, save the time.
The invention can directly separate the supernatant after the centrifugal separation of the accumulated liquid, does not need special equipment, is simple and quick, is convenient to extract and improves the working efficiency.
Claims (9)
1. A deposition layer extraction device for accumulated liquid pathological analysis comprises a box body (1), and is characterized in that a detachable cover (2) is installed at the upper end of the box body (1), and a rotatable disc (3) is installed at the center of the bottom of the box body (1); a plurality of buckles (4) are evenly distributed on the circumference of the disc (3), each buckle (4) is fixedly provided with a test tube (5), an opening of each test tube (5) faces inwards, a cylindrical shell (6) can be detachably mounted at the opening of each test tube (5), the inner diameter of each shell (6) is the same as that of each test tube (5), a piston (7) is mounted in each shell (6), a plurality of piston rods (8) penetrating through each shell (6) are fixedly arranged on the inner side of each piston (7), and each piston rod (8) can drive each piston (7) to reciprocate and reset inwards;
a hard suction pipe (9) is arranged on the piston (7), the outer end of the suction pipe (9) penetrates through the shell (6) and is arranged in the test tube (5), and the inner end of the suction pipe (9) is fixed with the piston (7) and extends out of the inner side of the piston (7); an air inlet pipe (10) with one end arranged inside the test tube (5) and the other end arranged outside the shell (6) penetrates through the shell (6), and a one-way air inlet valve (11) is arranged on the air inlet pipe (10); an exhaust port (12) is arranged on the outer edge surface of the shell (6), and a one-way liquid inlet valve (13) is arranged on the suction pipe (9);
a vertical guide rod (14) penetrates through the cover (2), a conical disc (15) with a downward small end is fixed at the lower end of the guide rod (14), the conical disc (15) is pressed, the outer edge surface of the conical disc (15) can extrude the piston rod (8), and the piston (7) drives the suction pipe (9) to move outwards simultaneously;
test tube (5) be the plastics material, pass through threaded connection between test tube (5) and casing (6) to the winding has sealed sticky tape.
2. The device for extracting a sediment layer in the pathological analysis of fluid accumulation as claimed in claim 1, wherein the inner end of the shell (6) is provided with a drain valve (16) through a pipeline.
3. An apparatus for extracting a sediment layer in the pathological analysis of an accumulated fluid according to claim 1, characterized in that the air inlet valve (11) can only allow air to enter the test tube (5), and the liquid inlet valve (13) can only allow liquid in the test tube (5) to enter the housing (6).
4. The device for extracting the precipitate layer in the pathological analysis of accumulated liquid as claimed in claim 1, wherein the bottom of the shell (6) is provided with a motor (17), and an output shaft of the motor (17) is fixedly connected with the center of the lower end surface of the disc (3).
5. The device for extracting the precipitate layer in the pathological analysis of accumulated liquid according to claim 1, wherein the joint between the suction tube (9) and the housing (6) and the joint between the piston rod (8) and the housing (6) are sealed by rubber rings.
6. The device for extracting a sediment layer in the pathological analysis of fluid accumulation as claimed in claim 1, wherein each shell (6) is also provided with a buckle (4).
7. The device for extracting a sediment layer in the pathological analysis of accumulated fluids as claimed in claim 1, wherein the buckle (4) comprises a vertical rod (18) fixed on the disc (3), and the upper end of the vertical rod (18) is fixedly provided with a hoop (19).
8. The device for extracting the deposition layer for the pathological analysis of accumulated liquid according to claim 1, wherein a plurality of bolts which can penetrate through the cover (2) are arranged on the upper end surface of the box body (1), and nuts are screwed on the bolts; the cover (2) is provided with a permanent magnet (20) capable of adsorbing the conical disc (15); scales are arranged on the guide rod (14), and the outer edge surface of the guide rod (14) is in threaded connection with an adjusting nut (21).
9. The effusion pathology analysis sediment layer extraction element of claim 1, characterized in that, the piston rod (8) on each piston (7) is arranged two from top to bottom, the inner end of each piston rod (8) is equipped with a ball (22), each piston rod (8) is equipped with a pressure spring (24) between the ball (22) and the housing (6); the two piston rods (8) are connected together via a connecting rod (23) to increase strength.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010074968.4A CN111141569B (en) | 2020-01-22 | 2020-01-22 | Hydrops class pathological analysis sediment layer extraction element |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010074968.4A CN111141569B (en) | 2020-01-22 | 2020-01-22 | Hydrops class pathological analysis sediment layer extraction element |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111141569A CN111141569A (en) | 2020-05-12 |
| CN111141569B true CN111141569B (en) | 2020-12-29 |
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ID=70526962
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010074968.4A Expired - Fee Related CN111141569B (en) | 2020-01-22 | 2020-01-22 | Hydrops class pathological analysis sediment layer extraction element |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111141569B (en) |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ZA948564B (en) * | 1993-11-19 | 1995-07-26 | Bristol Myers Squibb Co | Liquid separation apparatus and method |
| US20080164204A1 (en) * | 2007-01-08 | 2008-07-10 | Mehdi Hatamian | Valve for facilitating and maintaining separation of fluids and materials |
| EP2695655A1 (en) * | 2012-08-09 | 2014-02-12 | F. Hoffmann-La Roche AG | Multi-part device for extracting plasma from blood |
| KR101466762B1 (en) * | 2014-03-28 | 2014-11-28 | 이준석 | Central seperator and central seperating method and bowl for central seperator |
| CN204379861U (en) * | 2015-01-05 | 2015-06-10 | 浙江保尔曼生物科技有限公司 | Plasma separating unit |
| DE102016015569A1 (en) * | 2016-12-29 | 2018-07-05 | Heinz Rohrer | Device for receiving liquids and for the exact removal of individual phases of the absorbed liquid |
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2020
- 2020-01-22 CN CN202010074968.4A patent/CN111141569B/en not_active Expired - Fee Related
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| CN111141569A (en) | 2020-05-12 |
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Granted publication date: 20201229 |