CN209210802U - A kind of centrifuge tube - Google Patents

Abstract

The utility model relates to a kind of centrifuge tubes, including centrifugation tube body, handle, connector, sealing element and bottom cover, the bottom of the centrifugation tube body has base opening, the bottom cover is connected with the base opening, the connector is fixedly connected with the sealing element and is arranged in the centrifuge tube body, the inner wall of the sealing element and the centrifugation tube body is tightly connected, and the handle is inserted into the centrifugation tube body and is detachably connected with the connector.The centrifuge tube of the utility model may be implemented layering and take liquid and addition reagent, improves extraction efficiency and safety, saves equipment cost.

Description

A kind of centrifuge tube

Technical field

The utility model relates to medical instruments field, specially a kind of centrifuge tube.

Background technique

In bio-science field, centrifugation technique has obtained very extensive application.Centrifugation technique is protein, enzyme, nucleic acid And the separation of cell subfraction one of most common method and Biochemical Lab in commonly separate, purify or clear side Method.Sample suspension is contained in centrifuge tube under high speed rotation, since huge centrifugal force acts on, makes the molecule to suspend (such as precipitating of organelle, large biological molecule) with certain velocity sedimentation, to be separated with solution.

Centrifuge tube used at present is traditional pipe lid, pipe shaft structure, and the solution for needing to be centrifuged is added by liquid-transfering gun Enter into centrifuge tube, the solution after centrifugation is layered in centrifuge tube, if it is desired to obtaining lower layer's solution, needs to be siphoned away with liquid-transfering gun Layer solution, obtains the solution of lower layer.Pollution lower layer's solution is possible in operating process, and operating process is complicated.If it is desired to obtaining The solution for obtaining different levels needs to carry out multiple liquid feeding and packing.For example, during extracting and freezing fat stem cell usually The tissue that different levels are extracted after needing to be centrifuged, carries out multiple liquid feeding and packing.It needs repeatedly to replace centrifuge tube in the process, not only A large amount of uses of centrifuge tube are caused, while cumbersome, and increases the possibility of the pollution of the different levels solution in operating process.

Utility model content

The technical problem to be solved by the utility model is to provide a kind of centrifuge tubes, and layering may be implemented and take liquid.

The technical solution that the utility model solves above-mentioned technical problem is as follows: a kind of centrifuge tube, including centrifugation tube body, hand The bottom of handle, connector, sealing element and bottom cover, the centrifugation tube body has base opening, and the bottom cover is connected with the base opening, institute It states connector to be fixedly connected and be arranged in the centrifuge tube body with the sealing element, the sealing element and the centrifugation tube body Inner wall is tightly connected, and the handle is inserted into the centrifugation tube body and is connected with the connector.

The beneficial effects of the utility model are: centrifugation bottom opens up base opening, sealing element is arranged in centrifugation tubular body, and Handle is connect with sealing element by connector.The centrifuge tube of the utility model can by drawing handle, from base opening discharge or Inhalation solution, addition solution is not needed by other utensils such as liquid-transfering gun, and lower layer's solution after being easily centrifuged. When tissue after centrifugation is divided into multilayer, the tissue of different layers can be successively released from base opening, greatly reduces Multi-layer centrifugal group The step of knitting separation and time.Bottom cover is covered when centrifugation, and solution is avoided to overflow from base opening.The centrifuge tube of the utility model is at one Realize that layering takes liquid and addition reagent on centrifuge tube simultaneously, and tissue solution dispenses after being handled, improve extraction efficiency and Equipment cost is saved in safety.

Based on the above technical solution, the utility model can also do following improvement.

Further, the handle includes pushing body, catch bar and pusher head, and the promotion body and the pusher head pass through institute Catch bar connection is stated, the pusher head is detachably connected with the connector.

Beneficial effect using above-mentioned further scheme is that handle is connected by pusher head with the intracorporal connector of centrifuge tube It connects, body is pushed to connect with pusher head by catch bar, push body outside centrifuge tube body, by pushing body that can push hand Motion of handle, and then the sealing element being fixedly connected with connector is driven to move in tube body.When carrying out centrifugally operated, it can dismantle down Handle puts into a centrifuge convenient for centrifuge tube.

Specifically, the pusher head is fixedly connected with the connector；Or the pusher head and the connector it is detachable Connection.

Further, the pusher head includes at least two pushing parts, and at least two pushing part is pushed away with described respectively Lever is vertical.

Beneficial effect using above-mentioned further scheme be pusher head at least two pushing parts drive the connector and The sealing element movement, uniform force, stable connection.

Further, the pusher head includes four pushing parts, and four pushing parts are vertical with the catch bar respectively, phase Two adjacent pushing parts are mutually perpendicular to.

Beneficial effect using above-mentioned further scheme is four pushing parts in cross, drives the connector and described Sealing element movement, uniform force, stable connection.

Further, the connector includes positioning plate and connection ring, and the connection ring is fixedly connected with the sealing element, institute It states positioning plate to be fixedly connected with the connection ring, the positioning plate has the through-hole to match with the pusher head, the connection The inner ring of ring has the limiting section of the rotation angle for limiting the pushing part of at least one.

Beneficial effect using above-mentioned further scheme is the through-hole of pusher head insertion positioning plate, is inserted into connection ring, is turned Dynamic pusher head offsets with limiting section, and pusher head can not be extracted from the through-hole of positioning plate.Pusher head by with positioning plate and connection ring Cooperation, can be moved back and forth in centrifuge tube body with dynamic seal.Pusher head is to be detachably connected with positioning plate and connection ring, It needs to suck or be discharged the intracorporal solution fashionable dress upper handle of centrifuge tube, removes handle when being put into centrifuge centrifugation.The utility model Centrifuge tube while realizing that layering takes liquid and adds the function of reagent, dismantle lower handle in order to using in centrifuge.

Specifically, the thickness of the connection ring is greater than the thickness of the pusher head.

Further, the connector is lockplate, and the lockplate has locking through-hole compatible with the pusher head, The lockplate is fixedly connected with the sealing element, and the side that the lockplate is connect with the sealing element has what Open Side Down Fan-shaped lock slots.

Beneficial effect using above-mentioned further scheme is the locking through-hole of pusher head insertion lockplate, pusher head and sealing After part contact, pusher head is rotated, after pusher head turns over certain angle in fan-shaped lock slots, pusher head and lockplate are locked. Pusher head can move back and forth in centrifuge tube body with dynamic seal.Pusher head is to be detachably connected with lockplate, needs to suck Or the discharge intracorporal solution fashionable dress upper handle of centrifuge tube, handle is removed when being put into centrifuge centrifugation.The centrifuge tube of the utility model While realizing that layering takes liquid and adds the function of reagent, lower handle is dismantled in order to use in centrifuge.

Specifically, the depth of the lock slots is greater than the thickness of the pusher head.

It further, further include top cover, the top cover is connected to the top open part of the centrifugation tube body, and the top cover has The handle hole passed through for the handle.

Beneficial effect using above-mentioned further scheme is that top cover can be passed through for handle, and avoid quilt in centrifuge tube body Germ contamination guarantees the intracorporal gnotobasis of centrifuge tube.

Specifically, the top cover and the centrifugation tube body are interference fitted；Or the top cover and the centrifugation tube body are clamped.

Further, the centrifugation inboard wall of tube body has clamping portion, and the side of the top cover corresponds to the clamping portion and is provided with Clamping groove, the top cover and the centrifugation tube body are clamped.

Beneficial effect using above-mentioned further scheme is top cover and centrifugation tube body clamping, stable connection, while can be to prevent Connector, sealing element and top cover are detached from centrifugation tube body when being only centrifuged.

It further, further include top cap, the top cap is removably tightly connected with the top cover.

Beneficial effect using above-mentioned further scheme is to split out handle before being centrifuged, and top cap is covered on top cover, centrifuge tube Body good airproof performance.

Specifically, the top cap has lug boss identical with the handle hole shape size of the top cover, it is described convex The portion of rising and the handle hole are cooperatively connected.

Further, there is graduated scale on the side wall of the centrifugation tube body.

Beneficial effect using above-mentioned further scheme is sucked or is discharged quantitative using centrifuge tube according to graduated scale Solution.

Detailed description of the invention

Fig. 1 is a kind of structural schematic diagram of centrifuge tube of the utility model；

Fig. 2 is a kind of centrifuge tube cross-sectional view of the utility model；

Fig. 3 is a kind of handle arrangement schematic diagram of centrifuge tube of the utility model；

Fig. 4 is a kind of structural schematic diagram of centrifuge tube positioning plate of the utility model；

Fig. 5 is a kind of structural schematic diagram of centrifuge tube connection ring of the utility model；

Fig. 6 is a kind of structural schematic diagram of centrifuge tube lockplate of the utility model；

Fig. 7 is a kind of structural schematic diagram for being centrifuged seals of the utility model；

Fig. 8 is a kind of structural schematic diagram of centrifuge tube top cover of the utility model；

Fig. 9 is the structural schematic diagram that a kind of centrifuge tube of the utility model is centrifuged tube body；

Figure 10 is a kind of structural schematic diagram of centrifuge tube top cap of the utility model.

In attached drawing, parts list represented by the reference numerals are as follows:

1. handle, 101. push body, 102. catch bars, 103. pusher heads, 1031. pushing parts, 2. top covers, 3. centrifuge tubes Body, 4. positioning plates, 5. connection rings, 501. limiting sections, 6. sealing elements, 7. bottom covers, 8. top caps, 9. lockplates.

Specific embodiment

The principles of the present invention and feature are described below, example is served only for explaining the utility model, and It is non-to be used to limit the scope of the utility model.

Embodiment 1

As depicted in figs. 1 and 2, a kind of centrifuge tube, including centrifugation tube body 3, handle 1, connector, sealing element 6 and bottom cover 7, The bottom of the centrifugation tube body 3 has base opening, and the bottom cover 7 is connected with the base opening, the connector and the sealing element 6 It is fixedly connected and is arranged in the centrifugation tube body 3, the inner wall of the sealing element 6 and the centrifugation tube body 3 is tightly connected, described Handle 1 is inserted into the centrifugation tube body 3 and is connected with the connector.

Specifically, the centrifugation tube body 3 is hollow tube body, the side wall of the centrifugation tube body 3 is cylindrical tube shape, and bottom is Infundibulate.

Specifically, the connector is Nian Jie with the sealing element 6.

Specifically, the material of the sealing element 6 is rubber.

Preferably, the material of the centrifugation tube body 3 is medical plastic.

Specifically, having threaded hole among the bottom cover 7, there is external screw thread on the outside of the base opening, the bottom cover 7 with it is described Base opening is threadedly coupled.The overall diameter of bottom cover 7 is identical as the centrifugation outer diameter of tube body, and the outer surface of bottom cover 7 has anti-skid chequer.The bottom Mouth diameter is 0.5 centimetre.

As the further scheme of the present embodiment, as shown in figure 3, the handle 1 includes pushing body 101,102 and of catch bar Pusher head 103, the promotion body 101 are connect with the pusher head 103 by the catch bar 102, the pusher head 103 and institute Connector is stated to be detachably connected.

As the further scheme of the present embodiment, the pusher head 103 include at least two pushing parts 1031, at least two The pushing part 1031 it is vertical with the catch bar 102 respectively.

As the further scheme of the present embodiment, the pusher head 103 includes four pushing parts 1031, four promotions Portion 1031 is vertical with the catch bar 102 respectively, and the pushing part 1031 of adjacent two is mutually perpendicular to.

Specifically, four pushing parts 1031 of pusher head are perpendicular to catch bar 102 in cross and in the same plane.

As the further scheme of the present embodiment, as shown in Figure 4 and Figure 5, the connector includes positioning plate 4 and connection ring 5, the connection ring 5 is fixedly connected with the sealing element 6, and the positioning plate 4 is fixedly connected with the connection ring 5, the positioning Plate 4 has through-hole compatible with the pusher head 103, and the inner ring of the connection ring 5 is used to limit institute at least one State the limiting section 501 of the rotation angle of pushing part 1031.

Specifically, as shown in Figure 4 and Figure 5, positioning plate 4 has cross identical with 103 cross-sectional shape of pusher head, size The through-hole of shape.The thickness of the connection ring 5 is greater than the thickness of the pusher head 103.Pusher head 103 can turn in connection ring 5 It is dynamic.As shown in figure 5, the inner ring tool of connection ring 5 is there are four limiting section 501, limiting section 501 is from 5 inner ring of connection ring to center of circle side To the protrusion of extension, two adjacent limiting sections 501 are mutually perpendicular to.Connection ring 5 is Nian Jie with sealing element 6, positioning plate 4 and connection ring 5 bondings, the limiting section 501 and the through-hole of the cross shape of positioning plate 4 of connection ring 5 are staggered, each 501 center line of limiting section Angle with through hole center line is 45 degree.Pusher head 103 is inserted into the through-hole of positioning plate, and then is inserted into connection ring 5.Rotation Push body 101 that pusher head 103 is driven to rotate in connection ring 5, until the pushing part of pusher head 103 1031 and 501 phase of limiting section It supports, pushing part 1031 and the cross-hole of positioning plate 4 are staggered, and pushing part 1031 is limited not deviating from by positioning plate 4, handle 1 locks with positioning plate 4 and connection ring 5.It can be moved in centrifugation tube body 3 by drawing handle 1 with dynamic seal 6.

As the further scheme of the present embodiment, as shown in figure 8, further include top cover 2, the top cover 2 be connected to it is described from The top open part of heart tube body 3, the top cover 2 have the handle hole passed through for the handle 1.

Specifically, top cover 2 and the centrifugation interference fit of tube body 3 connect.Top cover 2 has and 103 cross-sectional shape of pusher head, big The handle hole of small identical cross shape.

Alternatively, as shown in figure 8,3 inner wall of centrifugation tube body has clamping portion, the side of the top cover 2 corresponds to institute It states clamping portion and is provided with clamping groove, the top cover and the centrifugation tube body 3 are clamped.

Specifically, as shown in figure 9, the diameter of centrifugation 3 top open part of tube body is avoided greater than the diameter of centrifugation tube body lower part Clamping portion hinders the intracorporal connector of centrifuge tube and sealing element disassembly.Facilitate centrifuge tube disassembly, cleaning, reuse.

It further include top cap 8 as shown in Figure 10 as the further scheme of the present embodiment, the top cap 8 and the top cover 2 It is dismountable to be tightly connected.

Specifically, splitting out handle 1 before centrifugation, top cap 8 is covered on top cover 2, guarantees the leakproofness of centrifugation tube body 3.It is described Top cap 8 has lug boss identical with the handle hole shape size of the top cover 2, and the lug boss is matched with the handle hole Close connection.

As the further scheme of the present embodiment, there is graduated scale on the side wall of the centrifugation tube body 3.

The volume size of the centrifuge tube of the present embodiment can need to be designed according to experiment.

Specific use process is as follows for the present embodiment:

Inhalation solution: removing top cap 8 and bottom cover 7, and the pusher head 103 of handle 1 is passed through to the handle hole of top cover 2, and it is fixed to pass through The cross-hole of position plate 4, and then be inserted into connection ring 5.Body 101 is pushed to be located at the outside of centrifugation tube body 3 at this time, rotation pushes Body 101 drives pusher head 103 to rotate in connection ring 5, until the pushing part of pusher head 103 1031 and limiting section 501 offset, pushes away Dynamic portion 1031 and the cross-hole of positioning plate 4 are staggered.Base opening protrudes into liquid to be centrifuged, pulls handle 1, pushing part 1031 by Limitation to positioning plate 4 can not deviate from, and then move with dynamic seal 6 away from base opening direction, suck liquid.According to scale Scale can accurately suck quantitative liquid.

Centrifugation: bottom cover 7 is covered.Rotational handle 1 makes pushing part 1031 be directed at the cross-hole of positioning plate 4, and handle 1 is pulled out Positioning plate 4 and top cover 2.Cover top cap 8.Centrifuge tube is put into a centrifuge, centrifugally operated is carried out.

The centrifuge tube of the utility model can be used for after being centrifuged needing the case where extracting multi-level tissue.Such as extracting fat The tissue of different levels is extracted after usually needing to be centrifuged during stem cell；Centrifugal blood obtain respectively red blood cell, leucocyte and Serum etc..

Discharge solution: removing top cap 8 and bottom cover 7, and the pusher head 103 of handle 1 is passed through to the handle hole of top cover 2, and it is fixed to pass through The cross-hole of position plate 4, and then be inserted into connection ring 5.Body 101 is pushed to be located at the outside of centrifugation tube body 3 at this time, rotation pushes Body 101 drives pusher head 103 to rotate in connection ring 5, until the pushing part of pusher head 103 1031 and limiting section 501 offset, pushes away Dynamic portion 1031 and the cross-hole of positioning plate 4 are staggered.Driving handle 1, and then transported with dynamic seal 6 towards close to base opening direction It is dynamic, it is discharged and collects the liquid of the bottom.In order to avoid the pollution of different levels solution, give up after the liquid discharge near solution layering It abandons, then proceedes to the liquid for collecting a upper level.

In the biggish situation of centrifugal speed, the bulky grain in some tissue homogenate can generate precipitating in centrifuge tube lower part. These precipitatings belong to semi-solid material, and sealing element 6 is mobile to base opening in centrifugation tube body 3, and pressure increases in tube body, this is practical new The centrifuge tube of type can precipitate the intracorporal lower part of discharge pipe easily.

The centrifuge tube of the utility model is disposable centrifuge tube, or the repeatedly centrifugation of centrifugation Reusability Pipe.

Embodiment 2

On the basis of embodiment 1, as shown in fig. 6, the connector can also be lockplate 9, the lockplate 9 has Locking through-hole compatible with the pusher head 103, the lockplate 9 are fixedly connected with the sealing element 6, the lockplate 9 The side connecting with the sealing element 6 has the fan-shaped lock slots that Open Side Down.

Lockplate 9 shown in Fig. 6 is view of the lockplate 9 towards 6 side of sealing element, and lockplate 9 is Nian Jie with sealing element 6. Lockplate 9 has cross-hole, the pusher head 103 of handle 1 is passed through to the handle hole of top cover 2, across the cross-hole of lockplate 9 Extremely it is in contact with sealing element 6.Body 101 is pushed to be located at the outside of centrifugation tube body 3 at this time, rotation pushes body 101 to drive pusher head 103 It rotates in the fan-shaped lock slots of lockplate 9, until the pushing part of pusher head 103 1031 and the side wall of lock slots offset, pushes Portion 1031 and the cross-hole of lockplate 9 are staggered, and pushing part 1031 is blocked by the top surface of fan-shaped lock slots, can not be from lockplate 9 Cross-hole in extract.Pusher head 103 and lockplate 9 lock, and drawing handle 1 drives lockplate 9 and sealing element 6 in centrifuge tube Movement in body 3 realizes that layering takes liquid and addition reagent.

The above is only the preferred embodiment of the present invention, is not intended to limit the utility model, all practical at this Within novel spirit and principle, any modification, equivalent replacement, improvement and so on should be included in the guarantor of the utility model Within the scope of shield.

Claims (10)

1. a kind of centrifuge tube, which is characterized in that including centrifugation tube body (3), handle (1), connector, sealing element (6) and bottom cover (7), the bottom of centrifugation tube body (3) has base opening, and the bottom cover (7) is connected with the base opening, the connector and institute It states sealing element (6) to be fixedly connected and be arranged in the centrifugation tube body (3), the sealing element (6) and centrifugation tube body (3) Inner wall is tightly connected, and the handle (1) is inserted into the centrifugation tube body (3) and is connected with the connector.

2. a kind of centrifuge tube according to claim 1, which is characterized in that the handle (1) includes pushing body (101), pushing Bar (102) and pusher head (103), the promotion body (101) are connect with the pusher head (103) by the catch bar (102), The pusher head (103) is detachably connected with the connector.

3. a kind of centrifuge tube according to claim 2, which is characterized in that the pusher head (103) includes at least two promotions Portion (1031), at least two pushing part (1031) are vertical with the catch bar (102) respectively.

4. a kind of centrifuge tube according to claim 3, which is characterized in that the pusher head (103) includes four pushing parts (1031), two vertical with the catch bar (102) respectively, adjacent pushing parts of four pushing parts (1031) (1031) it is mutually perpendicular to.

5. according to a kind of centrifuge tube of claim 3 or 4, which is characterized in that the connector includes positioning plate (4) and connection Ring (5), the connection ring (5) are fixedly connected with the sealing element (6), the positioning plate (4) and the connection ring (5) fixed company It connects, the positioning plate (4) has with the pusher head (103) compatible through-hole, and the inner ring of the connection ring (5) has at least The limiting section (501) of one rotation angle for limiting the pushing part (1031).

6. according to a kind of any one of claim 2-4 centrifuge tube, which is characterized in that the connector is lockplate (9), institute Stating lockplate (9) has with the pusher head (103) compatible locking through-hole, the lockplate (9) and the sealing element (6) It is fixedly connected, the side that the lockplate (9) connect with the sealing element (6) has the fan-shaped lock slots that Open Side Down.

7. a kind of centrifuge tube according to claim 1, which is characterized in that further include top cover (2), the top cover (2) is connected to The top open part of centrifugation tube body (3), the top cover (2) have the handle hole passed through for the handle (1).

8. a kind of centrifuge tube according to claim 7, which is characterized in that centrifugation tube body (3) inner wall has clamping portion, institute The side for stating top cover (2) corresponds to the clamping portion and is provided with clamping groove, and the top cover and the centrifugation tube body (3) are clamped.

9. a kind of centrifuge tube according to claim 7, which is characterized in that further include top cap (8), the top cap (8) with it is described Top cover (2) is removably tightly connected.

10. any one of -4 or 7-9 a kind of centrifuge tube according to claim 1, which is characterized in that centrifugation tube body (3) There is graduated scale on side wall.