CN111135961B - Centrifugal separation device for effusion in abdominal cavity - Google Patents
Centrifugal separation device for effusion in abdominal cavity Download PDFInfo
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- CN111135961B CN111135961B CN202010074967.XA CN202010074967A CN111135961B CN 111135961 B CN111135961 B CN 111135961B CN 202010074967 A CN202010074967 A CN 202010074967A CN 111135961 B CN111135961 B CN 111135961B
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- piston
- shell
- test tube
- effusion
- abdominal cavity
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
- B04B5/0421—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes pivotably mounted
Abstract
The invention relates to a centrifugal separation device for effusion in abdominal cavity, which effectively solves the problem that supernatant can not be directly extracted in the centrifugal process; the technical scheme includes that the test tube test device comprises a box body, wherein a rotatable disc is arranged in the box body, a test tube is fixed on the disc, a shell is installed at an opening of the test tube, a first piston is installed in the shell, a first piston rod is fixed on the inner side of the first piston, a hard suction tube is installed on the first piston, one end of the suction tube is arranged in the test tube, and the other end of the suction tube is arranged on the inner side of the first piston; an air inlet pipe is fixed on the shell, one end of the air inlet pipe is arranged in the test tube, the other end of the air inlet pipe is arranged on the outer side of the shell, and a one-way air inlet valve is arranged on the air inlet pipe; a one-way liquid inlet valve is arranged on the suction pipe; a joint positioned at the outer end of the shell is fixed on the shell, a piston cavity is arranged in the middle of the disc, a second piston is arranged in the piston cavity, and a second piston rod is rotatably connected to the upper part of the second piston; the bottom of the piston cavity is communicated with the plurality of joints one by one through the plurality of pipelines, the liquid supernatant extracting device is convenient to extract liquid supernatant, and the working efficiency is improved.
Description
Technical Field
The invention relates to the field of pathological analysis sample treatment, in particular to a centrifugal separation device for effusion in abdominal cavity.
Background
The pleural effusion is a common accompanying sign of various thoracic diseases, particularly neoplastic diseases, even appears for the first onset of patients, and the clear property of the pleural effusion has important significance for the diagnosis and treatment of the diseases; centrifugation, smear and staining are generally performed in general pathological examination, namely after pleural effusion is centrifuged, frozen sections of a sediment layer are subjected to conventional H staining, and the method has high repeatability, clear cells and clear diagnosis. Although the centrifuge that present hospital was equipped with can be fast carries out centrifugal treatment to the sample in the test tube, the sample after the centrifugation generally only needs to remain the precipitate layer, so just need after the centrifugation finishes, through long-time stationary precipitation after, separates, has prolonged the time of preparation sample.
At present, some centrifugal devices can directly pump away supernatant after centrifugation, but various problems exist, 1, common test tubes cannot be used, and special containers are needed, so that the cost is increased; 2. the sedimentation layer and the supernatant are easy to be mixed together instead of being extracted under the condition of high-speed rotation; 3. because the proportion of the supernatant is different due to different samples, the extraction 4 and the inclined or vertical arrangement of the general test tube 5 are needed according to the situation, the precipitation effect is poor, and the centrifugal force is not strong.
In summary, there is a need for a centrifuge device that effectively addresses the above-mentioned problems.
Disclosure of Invention
In view of the above situation, the present invention provides a centrifugal separation device for effusion in abdominal cavity, which can effectively solve the problem that supernatant cannot be directly extracted during centrifugation.
The technical scheme for solving the problem is that the device comprises a box body, wherein a detachable cover is arranged at the upper end of the box body, and a rotatable disc is arranged at the center of the bottom of the box body; a plurality of buckles are evenly distributed on the circumference of the disc, a test tube is fixed on each buckle, the opening of each test tube faces inwards, a cylindrical shell can be detachably mounted at the opening of each test tube, the inner diameter of the shell is the same as that of each test tube, a first piston is mounted in the shell, a plurality of first piston rods penetrating through the shell are fixed on the inner side of each first piston, and the first piston rods can drive the first pistons to reciprocate and reset inwards;
the first piston is provided with a hard suction pipe, the outer end of the suction pipe penetrates through the shell and is arranged in the test tube, and the inner end of the suction pipe is fixed with the first piston and extends out of the inner side of the first piston; an air inlet pipe is fixed on the shell, one end of the air inlet pipe is arranged in the test tube, the other end of the air inlet pipe is arranged on the outer side of the shell, and a one-way air inlet valve is arranged on the air inlet pipe; a one-way liquid inlet valve is arranged on the suction pipe;
the shell is fixedly provided with a joint positioned at the outer end of the shell, the middle of the disc is fixedly provided with a piston cavity, a second piston capable of moving up and down is arranged in the piston cavity, and the upper end surface of the second piston is rotatably connected with a vertical second piston rod; the upper end of the second piston rod is arranged above the cover and movably inserted with a cross rod; the bottom of the piston cavity is communicated with the plurality of joints one by one through a plurality of pipelines; the second piston rod is pulled upwards, and the first pistons synchronously move outwards.
The invention can directly separate the supernatant after the centrifugal separation of the accumulated liquid, does not need special equipment, is simple and quick, is convenient to extract the supernatant and improves the working efficiency.
Drawings
Fig. 1 is a front view of the present invention.
FIG. 2 is a front sectional view of the present invention.
FIG. 3 is a top cross-sectional view of the present invention (with the piston chamber removed).
Fig. 4 is a front sectional view of the test tube, the housing, the pipette, the first piston rod, and the like.
Fig. 5 is a state diagram of fig. 4.
Fig. 6 is a front sectional view of the piston chamber.
Fig. 7 is a perspective view of the matching relationship between the pipette, the first piston, and the first piston rod.
Detailed Description
The following describes in further detail embodiments of the present invention with reference to the accompanying drawings.
As shown in fig. 1 to 7, the invention comprises a box body 1, a detachable cover 2 is arranged at the upper end of the box body 1, and a rotatable disc 3 is arranged at the center of the bottom of the box body 1; a plurality of buckles 4 are uniformly distributed on the circumference of the disc 3, each buckle 4 is fixedly provided with a test tube 5, the opening of each test tube 5 faces inwards, a cylindrical shell 6 can be detachably mounted at the opening of each test tube 5, the inner diameter of each shell 6 is the same as that of each test tube 5, a first piston 7 is mounted in each shell 6, a plurality of first piston rods 8 penetrating through each shell 6 are fixed on the inner side of each first piston 7, and each first piston rod 8 can drive each first piston 7 to reciprocate and reset inwards;
a hard suction pipe 9 is arranged on the first piston 7, the outer end of the suction pipe 9 penetrates through the shell 6 and is arranged in the test tube 5, and the inner end of the suction pipe 9 is fixed with the first piston 7 and extends out of the inner side of the first piston 7; an air inlet pipe 10 with one end arranged inside the test tube 5 and the other end arranged outside the shell 6 is fixed on the shell 6, and a one-way air inlet valve 11 is arranged on the air inlet pipe 10; a one-way liquid inlet valve 13 is arranged on the suction pipe 9;
a joint 12 positioned at the outer end of the shell 6 is fixed on the shell 6, a piston cavity 14 is fixedly installed in the middle of the disc 3, a second piston 15 capable of moving up and down is installed in the piston cavity 14, and the upper end face of the second piston 15 is rotatably connected with a vertical second piston rod 20; the upper end of the second piston rod 20 is arranged above the cover 2 and movably inserted with a cross rod 21; the bottom of the piston cavity 14 is communicated with the plurality of joints 12 one by one through a plurality of pipelines 22; by pulling the second piston rod 20 upwards, the plurality of first pistons 7 will move outwards in a synchronized manner.
To facilitate drainage, the inner end of the housing 6 is fitted with a drain valve 16 via a conduit 22.
In order to achieve the pumping of the supernatant, the air inlet valve 11 only allows the gas to enter the inside of the test tube 5, and the liquid inlet valve 13 only allows the liquid in the test tube 5 to enter the housing 6.
In order to realize the rotation of the disc 3, a motor 17 is installed at the bottom of the shell 6, and an output shaft of the motor 17 is fixedly connected with the center of the lower end face of the disc 3.
In order to facilitate connection and guarantee the sealing of the test tube 5 and the shell 6, the test tube 5 is made of plastic, the test tube 5 is connected with the shell 6 through threads, and a sealing adhesive tape is wound on the test tube 5.
In order to realize fixation, the buckle 4 comprises a vertical rod 18 fixed on the disc 3, and an anchor ear 19 is fixedly arranged at the upper end of the vertical rod 18.
In order to realize the detachment of the cover 2, a plurality of bolts capable of penetrating through the cover 2 are installed on the upper end surface of the box body 1, and nuts are screwed on the bolts.
In order to facilitate the inward pulling of the first pistons 7, the number of the first piston rods 8 on each first piston 7 is two, the inner ends of the two first piston rods 8 are connected together through a connecting rod 23, and each piston rod is sleeved with a pressure spring 24 between the housing 6 and the connecting rod 23.
In order to facilitate the connection and the disconnection between the joint 12 and the pipeline 22, the part of the pipeline 22 connected with the joint 12 is made of rubber tube materials and can be directly and tightly sleeved on the joint 12.
In order to realize the rotary connection between the second piston rod 20 and the second piston 15, a convex block 25 is arranged on the upper end surface of the second piston 15, and a thrust bearing 26 is arranged between the lower end of the second piston rod 20 and the convex block 25.
In order to better realize the suction of the piston chamber 14, the upper end surface of the piston chamber 14 is provided with a through hole 27 which is the same as the outside, and the bottom of the piston chamber 14 is provided with a plurality of supporting rods 28.
In order to realize sealing, the joint between the suction tube 9 and the housing 6 and the sliding joint between the first piston rod 8 and the housing 6 are sealed by rubber rings.
For better fixing of the test tubes 5, each of said housings 6 is also fitted with a catch 4.
When the invention is used, the test tube 5 is vertically placed, and the effusion which needs to be centrifuged is put into the test tube 5, and the amount of the effusion is preferably that when the shell 6 is fixed on the test tube 5, the effusion just covers the end part of the suction tube 9. Then, the test tube 5 and the shell 6 need to be fixed on the hoop 19 of the disc 3 at the same time; the fixing position of the test tube 5 and the housing 6 is preferably such that the inner end of the first piston rod 8 is in surface contact with the outer edge of the piston chamber 14 in the unstressed state.
Then, the second piston rod 20 needs to be pressed downwards, so that the second piston rod 20 moves until the second piston rod contacts with the support rod 28 and cannot move; the conduit 22 in the piston chamber 14 is then butted up against the nipple 12.
Fixing each combination of the test tube 5 and the housing 6 in turn in the above-mentioned manner, then covering the cover 2, pulling out the cross bar 21 at the upper end of the second piston rod 20 when covering the cover 2, and making the second piston rod 20 penetrate the cover 2, and then fixing by bolts and nuts around; after the cap 2 is closed, the cross bar 21 may be inserted again into the upper end of the second piston rod 20, as shown in fig. 1. Thereafter, centrifugation may be performed.
During centrifugation, only the motor 17 needs to be turned on, so that high-speed centrifugation operation can be performed, and the second piston rod 20 is not pulled in the centrifugation process, so that the centrifugation effect is prevented from being influenced.
Because test tube 5 level is placed in this device, consequently can be great improvement test tube 5's centrifugal force, compare and arrange or vertical arranging in traditional slope, can accelerate the centrifugation.
When the centrifugation is carried out for a certain time, the precipitation layer and the supernatant can be layered, and the supernatant needs to be sucked away; specifically, the distance required to lift the second piston rod 20 is determined according to relevant records or experimental experiences.
Then, the second piston rod 20 can be lifted smoothly, and the lower end of the second piston rod 20 is rotatably connected with the second piston 15 through the convex block 25 and the thrust bearing 26; therefore, when the second piston rod 20 is held by hand, the second piston rod 20 cannot rotate, and the second piston rod 20 is conveniently lifted; when the second piston rod 20 is lifted, the lower end surface of the second piston 15 generates negative pressure and sucks the gas between the outer side surface of the first piston 7 and the shell 6; causing the first piston 7 to move outwards. When the first piston rod 8 is moved outwards, a negative pressure chamber is also formed between the inner side of the first piston 7 and the inner wall of the housing 6.
Simultaneously because straw 9 and first piston 7 fixed connection to the inner of straw 9 stretches out a small distance of the medial surface of first piston 7, consequently under the effect of the negative pressure between first piston 7 medial surface and the casing 6 inner wall, straw 9 can inhale the supernatant in the test tube 5 in the cavity between first piston 7 right side and the casing 6 inner wall, and test tube 5 is inside can inhale through intake pipe 10 this moment, has guaranteed to maintain atmospheric pressure in the test tube 5.
Because first piston 7 and straw 9 fixed connection, and the casing 6 internal diameter is the same with the internal diameter of test tube 5, consequently along with the removal of first piston 7, straw 9 also can outwards remove in step, has guaranteed that the tip of straw 9 can be arranged in the supernatant always to realize stable absorption supernatant.
When the second piston rod 20 is not being lifted, the first piston 7 also stops moving, at this time, the first piston rod 8 is not being subjected to outward suction, and at the same time, under the action of the pressure spring 24 on the first piston rod 8, the first piston 7 has a tendency of returning inward, but because the suction pipe 9 is provided with a one-way valve, supernatant liquid between the inner side surface of the first piston 7 and the inner side wall of the shell 6 cannot be discharged; thereby immobilizing the first piston 7 and ensuring that the supernatant is always inside the housing 6.
Then the motor 17 can be stopped, the test tube 5 and the shell 6 are taken out, and the sediment in the shell 6 is subjected to the next wind treatment; the supernatant liquid in the shell 6 needs to be poured out through a drain valve 16; the shell 6 is fully cleaned and disinfected, so that the next use is facilitated; disposable materials may also be used to ensure sample accuracy.
According to the device, the shell 6 is innovatively arranged at the opening of the test tube 5, the first piston 7, the suction tube 9 and the like are ingeniously designed, so that separated supernatant in the test tube 5 can be easily transferred into the shell 6, and rapid and accurate separation is realized; only precipitates are left in the test tube 5, so that the next treatment such as frozen section and the like can be conveniently and rapidly carried out; the working efficiency is improved.
The 5 levels of test tube are placed in this device, can improve centrifugal force, and the centrifugal process accelerates, and simultaneously under high-speed centrifugal's state, directly separate, guaranteed that supernatant and precipitate layer are in the state of alternate segregation always, have guaranteed the accuracy of proposing.
In the device, the purpose of moving the plurality of first piston rods 8 is realized by lifting the second piston rods 20, so that the operation is convenient, simple and accurate; can once only aspirate the supernatant in a plurality of test tubes 5 in step, great improvement efficiency, save the time.
The invention can directly separate the supernatant after the centrifugal separation of the accumulated liquid, does not need special equipment, is simple and quick, is convenient to extract and improves the working efficiency.
Claims (8)
1. A centrifugal separation device for accumulated liquid in abdominal cavity comprises a box body (1), and is characterized in that a detachable cover (2) is arranged at the upper end of the box body (1), and a rotatable disc (3) is arranged at the center of the bottom of the box body (1); the disc (3) is evenly provided with a plurality of buckles (4) on the circumference, each buckle (4) is fixed with a test tube (5), the opening of each test tube (5) faces inwards, the opening of each test tube (5) can be detachably provided with a cylindrical shell (6), the inner diameter of each shell (6) is the same as that of each test tube (5), a first piston (7) is arranged in each shell (6), the inner side of each first piston (7) is fixed with a plurality of first piston rods (8) penetrating through the corresponding shell (6), and each first piston rod (8) can drive the corresponding first piston (7) to reciprocate and reset inwards;
a hard suction pipe (9) is arranged on the first piston (7), the outer end of the suction pipe (9) penetrates through the shell (6) and is arranged in the test tube (5), and the inner end of the suction pipe (9) is fixed with the first piston (7) and extends out of the inner side of the first piston (7); an air inlet pipe (10) with one end arranged inside the test tube (5) and the other end arranged outside the shell (6) is fixed on the shell (6), and a one-way air inlet valve (11) is arranged on the air inlet pipe (10); a one-way liquid inlet valve (13) is arranged on the suction pipe (9);
a joint (12) positioned at the outer end of the shell (6) is fixed on the shell (6), a piston cavity (14) is fixedly installed in the middle of the disc (3), a second piston (15) capable of moving up and down is installed in the piston cavity (14), and the upper end face of the second piston (15) is rotatably connected with a vertical second piston rod (20); the upper end of the second piston rod (20) is arranged above the cover (2) and movably inserted with a cross rod (21); the bottom of the piston cavity (14) is communicated with the plurality of joints (12) one by one through a plurality of pipelines (22); the second piston rod (20) is pulled upwards, and the first pistons (7) move outwards synchronously;
the inner end of the shell (6) is provided with a drain valve (16) through a connecting pipe;
the air inlet valve (11) only enables gas to enter the test tube (5), and the liquid inlet valve (13) only enables liquid in the test tube (5) to enter the shell (6).
2. A centrifugal separation device for effusion from abdominal cavity according to claim 1, characterized in that the bottom of the housing (6) is equipped with a motor (17), the output shaft of the motor (17) is fixedly connected with the center of the lower end surface of the disc (3).
3. A centrifugal separation device for effusion of abdominal cavity according to claim 1, wherein said test tube (5) is made of plastic material, the test tube (5) and the housing (6) are connected by screw thread and wound with sealing tape.
4. A centrifugal separation device for effusion from abdominal cavity according to claim 1, characterized in that said clasp (4) comprises a vertical rod (18) fixed on the disc (3), the upper end of the vertical rod (18) is fixedly mounted with a hoop (19).
5. The centrifugal separation device for the effusion of the abdominal cavity according to claim 1, characterized in that the first piston rods (8) on each first piston (7) are uniformly distributed and arranged in two up and down directions, the inner ends of the two first piston rods (8) are connected together through a connecting rod (23), and each piston rod is sleeved with a pressure spring (24) positioned between the housing (6) and the connecting rod (23).
6. A centrifugal separator for hydrops in abdominal cavity according to claim 1, wherein the connection part of said tube (22) and said connector (12) is made of rubber tube and can be directly and tightly sleeved on said connector (12).
7. A centrifugal separator for effusion from abdominal cavity according to claim 1, characterized in that the second piston (15) is provided with a bump (25) at its upper end, and that the second piston rod (20) is mounted between its lower end and the bump (25) via a thrust bearing (26).
8. A centrifugal separator for effusion from abdominal cavity according to claim 1, characterized in that the upper end surface of said piston chamber (14) is provided with through holes (27) identical to the outside, and the bottom of the piston chamber (14) is provided with a plurality of struts (28).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010074967.XA CN111135961B (en) | 2020-01-22 | 2020-01-22 | Centrifugal separation device for effusion in abdominal cavity |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010074967.XA CN111135961B (en) | 2020-01-22 | 2020-01-22 | Centrifugal separation device for effusion in abdominal cavity |
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| Publication Number | Publication Date |
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| CN111135961A CN111135961A (en) | 2020-05-12 |
| CN111135961B true CN111135961B (en) | 2021-02-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202010074967.XA Active CN111135961B (en) | 2020-01-22 | 2020-01-22 | Centrifugal separation device for effusion in abdominal cavity |
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| CN113072018B (en) * | 2021-03-17 | 2022-12-13 | 杭州博日科技股份有限公司 | Molecular detection centrifugal switch cover device |
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| EP2292334A3 (en) * | 2009-09-08 | 2012-04-25 | Andreas Hettich GmbH & Co. KG | Centrifuge for separating blood, fluidically communication containers therefore and method for generating high concentration thrombocyte concentrate from blood |
| KR101466762B1 (en) * | 2014-03-28 | 2014-11-28 | 이준석 | Central seperator and central seperating method and bowl for central seperator |
| CN106994396A (en) * | 2017-05-23 | 2017-08-01 | 武汉朗克医疗器械有限公司 | PRP continuous centrifugal separation devices and separation method |
| CN107413536A (en) * | 2012-02-15 | 2017-12-01 | 肯西纳什公司 | Centrifuge |
| CN109562394A (en) * | 2016-07-19 | 2019-04-02 | 李晙硕 | The method for being centrifugated container and the substance in mobile centrifuge separation container |
| CN110433969A (en) * | 2018-05-04 | 2019-11-12 | 百泽生医科技股份有限公司 | Liquid separating appts and its application method |
-
2020
- 2020-01-22 CN CN202010074967.XA patent/CN111135961B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2292334A3 (en) * | 2009-09-08 | 2012-04-25 | Andreas Hettich GmbH & Co. KG | Centrifuge for separating blood, fluidically communication containers therefore and method for generating high concentration thrombocyte concentrate from blood |
| CN107413536A (en) * | 2012-02-15 | 2017-12-01 | 肯西纳什公司 | Centrifuge |
| KR101466762B1 (en) * | 2014-03-28 | 2014-11-28 | 이준석 | Central seperator and central seperating method and bowl for central seperator |
| CN109562394A (en) * | 2016-07-19 | 2019-04-02 | 李晙硕 | The method for being centrifugated container and the substance in mobile centrifuge separation container |
| CN106994396A (en) * | 2017-05-23 | 2017-08-01 | 武汉朗克医疗器械有限公司 | PRP continuous centrifugal separation devices and separation method |
| CN110433969A (en) * | 2018-05-04 | 2019-11-12 | 百泽生医科技股份有限公司 | Liquid separating appts and its application method |
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Inventor after: Huang Diwen Inventor after: Cui Shuzhong Inventor after: Zhu Wei Inventor before: Zhu Wei |
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Effective date of registration: 20210204 Address after: 510080 4th floor, building 1, No.1 Keyuan Road, Baiyun District, Guangzhou City, Guangdong Province Applicant after: GUANGZHOU BRIGHT MEDICAL TECHNOLOGY Co.,Ltd. Address before: 730030 No. 222 Tianshui South Road, Chengguan District, Lanzhou, Gansu Applicant before: LANZHOU University |
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