CN111139244A - Populus tomentosa MODD1 gene and application thereof - Google Patents

Abstract

The invention relates to the field of biotechnology, in particular to a populus tomentosa MODD1 gene and application thereof, wherein the nucleotide sequence of the populus tomentosa MODD1 gene obtained by amplification is shown as SEQ ID NO. 1. According to the invention, the populus tomentosa MODD1 gene is transformed into arabidopsis thaliana through agrobacterium GV3101, and the drought resistance of arabidopsis thaliana with the over-expressed populus tomentosa MODD1 gene is improved, and the content of abscisic acid is improved, so that the invention can conclude that the populus tomentosa MODD1 gene can positively regulate the drought resistance of plants, and the drought resistance of the plants can be effectively improved by improving the expression level of the populus tomentosa MODD1 gene. The populus tomentosa MODD1 gene provided by the invention can be used for breeding drought-resistant strains of populus tomentosa and various crops.

Description

Populus tomentosa MODD1 gene and application thereof

Technical Field

The invention relates to the technical field of biology, in particular to a populus tomentosa MODD1 gene and application thereof.

Background

Terrestrial plants develop various drought-resistant functions in long-term adaptive evolution, but are still threatened by drought due to climate reasons. Drought affects not only the growth metabolic process of plants, but also the yield and reproduction of commercial crops, and severe drought stress can cause plant death. Therefore, the method has important significance for the research of the drought resistance of plants.

The Populus mauritiana MODD1 gene belongs to the NINJA (novel Interactor of JAZ proteins) protein family, which is an interaction protein of JAZ protein found in plants. The activity of the NINJA protein as a transcriptional repressor is mediated by a functional TPL binding domain upstream of this domain. Studies in plants have been shown to be transcriptional repression, as in arabidopsis AFP1 and AFP2 participate in drought regulation by binding to ABI5, negatively regulating ABA signaling, whereas ABF3 is up-regulated by ABA induction (Garcia et al, 2008). In rice, MODD protein, a NINJA family member, inhibits OsbZIP46 activity, and promotes OsbZIP46 degradation to inhibit drought resistance of rice (Tang et al, 2016). In addition to drought resistance, NINJA is involved in other signaling pathways, such as in arabidopsis, where it can interact with TOPLESS to negatively regulate the jasmonate signaling pathway (Pauwels et al, 2010).

Disclosure of Invention

The invention aims to improve the drought resistance of plants by using MODD1 gene derived from Chinese white poplar, and provides the MODD1 gene of Chinese white poplar and application thereof.

In order to achieve the purpose, the cDNA sequence of the populus tomentosa MODD1 gene is screened according to the annotation of the populus tomentosa genome, oligonucleotide primers are designed and synthesized according to the sequence, the cDNA after reverse transcription of the mRNA of the populus tomentosa is taken as a template, the full-length cDNA sequence of the populus tomentosa MODD1 gene is cloned and obtained and is named as PtMODD1, the full-length cDNA sequence is constructed on an entry vector, then the full-length cDNA sequence is constructed on a plant expression vector through recombination reaction, a positive clone is screened, and the Arabidopsis thaliana is infected after agrobacterium transformation (GV 3101).

After the treatment, the experimental results show that the transgenic plants do not have poor agronomic characters under the drive of the CaMV35S promoter of PtMODD1 derived from populus tomentosa, and the drought resistance of the PtMODD 1-transformed Arabidopsis is obviously improved. Arabidopsis thaliana is a model plant, and genes which can play a role in Arabidopsis thaliana have similar effects in various crops, so that PtMODD1 can be used for drought resistance of populus tomentosa and various crops.

Specifically, the technical scheme of the invention is as follows:

in a first aspect, the present invention provides a populus tomentosa MODD1 gene, wherein the nucleotide sequence of the populus tomentosa MODD1 gene is:

i) 1, SEQ ID NO; or

ii) the nucleotide sequence shown in SEQ ID No.1 is substituted, deleted and/or added with one or more nucleotides to encode the nucleotide sequence of the same functional protein.

The invention further provides a protein coded by the populus tomentosa MODD1 gene, wherein the amino acid sequence of the protein is as follows:

1) an amino acid sequence shown as SEQ ID No. 2; or

2) The amino acid sequence of the protein with the same function is obtained by replacing, inserting or deleting one or more amino acids in the amino acid sequence shown in SEQ ID No. 2.

The invention further provides a biological material containing the populus tomentosa MODD1 gene, wherein the biological material is one or more of a vector, a transgenic cell line, an engineering bacterium, a host cell or an expression cassette.

In a second aspect, the invention provides an application of the populus tomentosa MODD1 gene, or the coding protein thereof, or a biological material containing the same in regulating and controlling the drought resistance of plants.

The invention further provides the application of the populus tomentosa MODD1 gene or the coding protein thereof or the biological material containing the populus tomentosa MODD1 gene in plant genetic breeding or transgenic plant preparation.

The application specifically comprises the step of improving the drought resistance of the plants by improving the expression level of the populus tomentosa MODD1 gene.

Preferably, the increase of the expression level of the populus tomentosa MODD1 gene is the overexpression of the populus tomentosa MODD1 gene in the plant.

The invention further provides application of the populus tomentosa MODD1 gene or the coding protein thereof or biological material containing the populus tomentosa MODD1 gene in improving the content of abscisic acid in plants.

In the above application, the plant may be rice, arabidopsis, canola, soybean, cotton, wheat, corn or poplar.

In a third aspect, the present invention provides a method for regulating drought resistance of a plant, comprising: regulating and controlling the expression level of Chinese white poplar MODD1 gene in plant;

the nucleotide sequence of the populus tomentosa MODD1 gene is as follows:

i) 1, SEQ ID NO; or

ii) the nucleotide sequence shown in SEQ ID No.1 is substituted, deleted and/or added with one or more nucleotides to encode the gene of the same functional protein.

Further, drought resistance of the plant is improved by increasing the expression level of the populus tomentosa MODD1 gene in the plant, preferably by increasing the content of abscisic acid in the plant; alternatively, drought-resistant lines are cultivated by crossing the lines over expressing the populus tomentosa MODD1 gene with other lines.

Further, the construction method of the line over expressing the populus tomentosa MODD1 gene comprises the following steps:

the populus tomentosa MODD1 gene is connected to a plant expression vector and is obtained by transforming a wild type strain through agrobacterium GV 3101.

The invention provides a populus tomentosa MODD1 gene and application thereof, and has the following beneficial effects:

the invention provides an arabidopsis thaliana strain for constructing over-expression populus tomentosa MODD1 gene, and the comparison with wild type strains shows that under the drive of CaMV35S promoter, the MODD1 gene from populus tomentosa can not generate undesirable agronomic characters in transgenic plants, and the arabidopsis thaliana drought resistance of the transgenic populus tomentosa MODD1 gene is obviously improved. The arabidopsis thaliana is a model plant, and genes which can play a role in arabidopsis thaliana have similar effects in various crops, so that the populus tomentosa MODD1 gene provided by the invention can be used for breeding drought-resistant strains of populus tomentosa and various crops.

Drawings

FIG. 1 is a plasmid map of plasmid pGWC-PtMODD1 obtained by ligating the Populus tomentosa MODD1 gene to an entry vector, provided in example 1 of the present invention;

FIG. 2 is a plasmid map of plasmid pPtMODD1 obtained by ligating the Populus tomentosa MODD1 gene to a plant expression vector, according to example 1 of the present invention;

FIG. 3 is a schematic diagram showing the comparison of drought resistance between Arabidopsis thaliana (OE-PtMODD1-1 and OE-PtMODD1-2) and wild Arabidopsis thaliana (Col0) heterologously expressing the Populus tomentosa MODD1 gene in a consistent growth period, which are provided in example 3 of the present invention;

FIG. 4 is a schematic diagram showing the comparison of the expression levels of abscisic acid (ABA), Malondialdehyde (MDA) and proline (Pro) in Arabidopsis thaliana (OE-PtMODD1-1 and OE-PtMODD1-2) and wild type Arabidopsis thaliana (Col0) which are used for heterologous expression of Chinese white poplar MODD1 gene and have consistent growth period, which is provided by the embodiment 4 of the invention.

Detailed Description

The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.

Example 1

EXAMPLE 1 obtaining of MODD1 Gene of Populus tomentosa and construction of expression vector

1. Extraction of Populus tomentosa RNA

(1) Taking the example of the extraction of RNA from leaves as sample treatment, taking fresh leaves to fully grind in liquid nitrogen, wherein the grinding needs to be rapid;

(2) adding 500 μ L lysis solution (containing 5 μ L β -mercaptoethanol), vortex shaking, mixing, centrifuging at 12,000rpm for 2min, and transferring the supernatant to another centrifuge tube;

(3) adding 250 μ L of anhydrous ethanol, mixing (precipitate may appear), transferring the obtained solution and precipitate into adsorption column CR3 (adsorption column in collection tube), centrifuging at 12000 rpm for 1min, removing waste liquid, and placing adsorption column CR3 back into the collection tube;

(4) adding 350 μ L deproteinized solution RW1 into adsorption column CR3, centrifuging at 12000 rpm for 1min, discarding waste liquid, and placing adsorption column CR3 back into the collection tube;

(5) preparation of Dnase I working solution: putting 10 mu L of DNase I storage solution into a new RNase-free centrifuge tube, adding 70 mu L of RDD solution, and gently and uniformly mixing;

(6) adding 80 μ L DNase I working solution into the center of the adsorption column CR3, and standing at room temperature for 15 min;

(7) adding 500 μ L deproteinized solution RW1 into the center of adsorption column CR3, centrifuging at 12,000rpm for 1min, discarding the waste liquid, and placing the adsorption column back into the collection tube;

(8) adding 500 μ L of rinsing solution RW (containing ethanol) into adsorption column CR3, standing at room temperature for 2min, centrifuging at 12000 rpm for 1min, removing waste liquid, and placing adsorption column CR3 back into the collection tube;

(9) repeating the previous step;

(10) centrifuging at 12000 rpm for 2min, and discarding waste liquid. Placing the adsorption column CR3 at room temperature for several minutes to completely air-dry the residual eluent of the adsorption column;

(11) transferring the adsorption column CR3 into a new RNase-Free centrifuge tube, adding 60 μ L ddH₂O, standing at room temperature for 2min, and centrifuging at 12000 rpm for 2min to obtain an RNA solution.

2. Synthesis of Populus tomentosa cDNA

(1) Adding an RNA template, a primer and an RNase-free Water, uniformly mixing, incubating for 5 minutes at 65 ℃, and carrying out ice bath for 2 minutes;

(2) add 1. mu.L Random Primer, 10. mu.L Reaction Mix, 1. mu.L Transcript Enzyme Mix, 1. mu.L gDNA Remover;

(3) lightly mixing uniformly;

(4) after incubation at 25 ℃ for 10 minutes, incubation at 42 ℃ for 15 minutes;

(5) the Transcript Enzyme and gDNA Remover were inactivated by heating at 85 ℃ for 5 seconds.

3. Construction of Populus tomentosa MODD1 gene expression vector

Obtaining a Populus mauritiana MODD1 cDNA sequence according to a Populus mauritiana genomic database sequence, designing upstream and downstream amplification primers according to the sequence:

forward.5’-ATGGGAGAAACAAACGAGAATAG-3’，

reverse.5’-CACAAAGGACGGACCAGAAGAG-3’；

using Populus tomentosa cDNA as template, and pfu enzyme (Trans)

FastPfu DNA Polymerase) amplified the populus tomentosa MODD1 gene, and the obtained gene sequence was designated PtMODD 1. The amplification procedure was 2min pre-denaturation at 98 ℃, 30s 60 ℃, 1min 72 ℃, for 35 cycles. The PCR product was purified with a kit (Beijing Quanji Biotech Co., Ltd.) and used.

The PCR product was ligated to an entry vector (named pGWC-PtMODD1, FIG. 1 is the ligated plasmid map) using the In-fusion system, specifically including 1. mu.L of digested pGWCm (100 ng/. mu.L, digested with AhdI), 1. mu.L of LPCR product (80 ng/. mu.L), 2. mu.L of In-fusion Mix, 50-60min at 50 ℃ and E.coli DH5 α, and positive clones were selected by PCR identification.

After sequencing identification, the gene is constructed on a plant expression vector pHZM 69 through a Gateway system and is named as pPtMODD1 (a plasmid map is shown in figure 2), the promoter of PtMODD1 is CaMV35S, and the screening markers of the transformed plants in the vector are hygromycin and Basta. The recombinant plasmid is transformed into agrobacterium GV3101, and is identified by PCR for later use.

Example 2 genetic transformation of Populus tomentosa MODD1 Gene and screening of Positive transgenic lines

In this example, Arabidopsis thaliana was genetically transformed by the floral dip method, which specifically comprises the following steps:

agrobacterium harboring the pPtMODD1 plasmid constructed in example 1 was cultured in LB liquid medium (supplemented with 50mg/L kanamycin, 50mg/L gentamicin, and 200mg/L rifampicin)Culturing in medium until OD is 0.8, centrifuging at 10000 rpm for 5min, collecting thallus, and suspending with equal volume of suspension (10mM MgCl)₂5% sucrose), the cells were collected by centrifugation, suspended in a suspension until the OD became 1.0, and 0.005% Silwet L-77 was added to transform arabidopsis thaliana once at the early stage of flowering, followed by dipping once more at intervals of 7 days. After the seeds are mature, collecting T0 generation seeds, planting the seeds in a culture dish, spraying Basta (0.3%) for screening 10 days after seedling emergence, and spraying once again at an interval of 5 days; after PCR identification, T1 generation positive seedlings are transplanted into a nutrition pot for culture, and seeds are harvested after maturation.

Example 3 evaluation of drought resistance of transgenic Populus tremuloides MODD1 Gene

This example compares arabidopsis thaliana (prepared as described in example 2) overexpressing populus tomentosa MODD1 gene, and Col0 wild-type arabidopsis thaliana, which were grown at a consistent growth period, with a control group by drought treatment (without watering) for 14 days under the same greenhouse conditions, and photographs were taken of the comparison results. As shown in FIG. 3, in this example, it was found that the Arabidopsis thaliana (OE-PtMODD1 and OE-PtMODD1) overexpressing the Populus tomentosa MODD1 gene hardly suffered from wilting, while the control group (Col0) suffered from wilting significantly, and the results showed that the Populus tomentosa MODD1 gene significantly promoted the improvement of drought resistance of Arabidopsis thaliana.

Example 4 analysis of physiological indices of MODD1 Gene of Populus tomentosa

In this embodiment, a kit (enzyme immunoassay) is used to determine the levels of various indexes of plants in a specimen by using a double antibody sandwich method, and the specific steps are as follows:

coating a microporous plate with purified plant abscisic acid (ABA), Malondialdehyde (MDA) and proline (Pro) capture antibodies to prepare solid-phase antibodies, sequentially adding the ABA, MDA and Pro into the coated micropores, combining with HRP-labeled detection antibodies to form antibody, antigen and enzyme-labeled antibody complexes, and adding a substrate TMB for color development after thorough washing. TMB is converted to blue by the catalysis of HRP enzyme and to the final yellow by the action of acid. The shade of the color is positively correlated with the ABA, MDA and Pro of the plants in the sample. Measuring absorbance (OD value) by using an enzyme-labeling instrument at the wavelength of 450nm, and calculating the content of each index of the plant in the sample through a standard curve, wherein the method comprises the following specific steps:

(1) sample adding of the standard: setting standard substance holes and sample holes, wherein 50 mu L of standard substances with different concentrations are added into the standard substance holes respectively;

(2) sample adding: blank holes (the blank reference holes are not added with the sample and the enzyme labeling reagent, and the rest steps are operated in the same way) and sample holes to be detected are respectively arranged. 40 mu L of sample diluent is added into sample holes to be detected on the enzyme-labeled coated plate, and then 10 mu L of sample to be detected is added (the final dilution of the sample is 5 times). Adding a sample to the bottom of the hole of the enzyme label plate, keeping the sample from touching the hole wall as much as possible, and slightly shaking and uniformly mixing the sample and the hole wall;

(3) adding an enzyme: adding 100 mu L of enzyme-labeled reagent into each hole except for blank holes;

(4) and (3) incubation: sealing the plate with sealing plate film, and incubating at 37 deg.C for 60 min;

(5) preparing liquid: diluting 20 times of the concentrated washing liquid with 20 times of distilled water for later use;

(6) washing: carefully uncovering the sealing plate film, discarding liquid, spin-drying, filling washing liquid into each hole, standing for 30sec, discarding, repeating the steps for 5 times, and patting to dry;

(7) color development: adding 50 μ L of color-developing agent A into each well, adding 50 μ L of color-developing agent B, shaking gently, mixing, and developing at 37 deg.C in dark for 15 min;

(8) and (4) terminating: adding 50 mu L of stop solution into each well to stop the reaction (at the moment, the blue color immediately turns to yellow);

(9) and (3) determination: the absorbance (OD value) of each well was measured sequentially at a wavelength of 450nm with the blank well being zeroed. The determination should be performed within 15min after the addition of the stop solution.

The results shown in FIG. 4 were obtained by the above experiments (OE-PtMODD1 represents Arabidopsis thaliana heterologously expressing Chinese white poplar MODD1 gene, Col0 represents wild type), and the Arabidopsis thaliana heterologously expressing Chinese white poplar MODD1 gene has increased abscisic acid content and no difference in proline and malondialdehyde content compared with wild type.

Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Sequence listing

<110> institute of genetics and developmental biology of Chinese academy of sciences

China institute of forestry science and research

<120> Chinese white poplar MODD1 gene and application thereof

<130>KHP191116907.5

<160>4

<170>SIPOSequenceListing 1.0

<210>1

<211>1071

<212>DNA

<213>Populus tomentosa

<400>1

atgggagaaa caaacgagaa tagaaatagg agcagcagca gcagtagaag tagcagagta 60

atggagagtc tttctttgga tattaacagg tacccaagag atctattaca aagattcatg 120

tcaagtgacg cgcagcaata tcaaacagca acaagtgatg gagaagaaac agaggagatt 180

gaattaaatc ttggtctatc acttggtgga agatttgggg ttgacaagtc ttcaaagaaa 240

ctaacgagat cttcatcaat agctggttca ataccattgt tgagagacta cgatgcttta 300

accactccac ctgcatctta tcctcttcta atgaggactt cttctttgcc tacggagaca 360

gaggaggagt ggaggaagcg gaaggaaatg cagagtttga ggagaatgga agccaagaga 420

aggcgaagtg aaaagcaaag gaatttgaga ggagagttga gtctggagga agtcaaattg 480

aataaaggga attgggtgcc gacgtgggct agtaagcaga gtggtgttgt gaatagaggt 540

aataatctgg cagggcagca acagcagcag caagcgtcac aagggtccgt ggagtctcaa 600

ggagggagct cttctggatt atcagaaatg gtgagcaagc ctgttcaagg atcaagcagt 660

ggtggtgaag cccgaagtcc ctcaagtaac cagtctttgc aggaacgagg cagtcaagaa 720

gatttagatt cttctgggac aaagaaaaat gaaaatgcgt gcagagcctc cagtacagaa 780

atggagaact tttccaagaa gcttgattct gcagaaaata tagggaggga aattgggacc 840

aatgcaatgg aagatatgcc ttgtgtgttt acaaaaggag acggtcctaa tgggagaaga 900

gtggatggca tcctgtacaa gtatggcaag ggagaggaag taaggatcat gtgcgtctgc 960

catggcagct ttctctcccc cgcagagttc gtgaagcatg caggtggcag tgatgttaat 1020

caccctctta gacacatagt gataaactct tctggtccgt cctttgtgta g 1071

<210>2

<211>357

<212>PRT

<213>Populus tomentosa

<400>2

Met Gly Glu Thr Asn Glu Asn Arg Asn Arg Ser Ser Ser Ser Ser Arg

1 5 10 15

Ser Ser Arg Val Met Glu Ser Leu Ser Leu Asp Ile Asn Arg Tyr Pro

20 25 30

Arg Asp Leu Leu Gln Arg Phe Met Ser Ser Asp Ala Gln Gln Tyr Gln

35 40 45

Thr Ala Thr Ser Asp Gly Glu Glu Thr Glu Glu Ile Glu Leu Asn Leu

50 55 60

Gly Leu Ser Leu Gly Gly Arg Phe Gly Val Asp Lys Ser Ser Lys Lys

65 70 75 80

Leu Thr Arg Ser Ser Ser Ile Ala Gly Ser Ile Pro Leu Leu Arg Asp

85 90 95

Tyr Asp Ala Leu Thr Thr Pro Pro Ala Ser Tyr Pro Leu Leu Met Arg

100 105 110

Thr Ser Ser Leu Pro Thr Glu Thr Glu Glu Glu Trp Arg Lys Arg Lys

115 120 125

Glu Met Gln Ser Leu Arg Arg Met Glu Ala Lys Arg Arg Arg Ser Glu

130 135 140

Lys Gln Arg Asn Leu Arg Gly Glu Leu Ser Leu Glu Glu Val Lys Leu

145 150 155 160

Asn Lys Gly Asn Trp Val Pro Thr Trp Ala Ser Lys Gln Ser Gly Val

165 170 175

Val Asn Arg Gly Asn Asn Leu Ala Gly Gln Gln Gln Gln Gln Gln Ala

180 185 190

Ser Gln Gly Ser Val Glu Ser Gln Gly Gly Ser Ser Ser Gly Leu Ser

195 200 205

Glu Met Val Ser Lys Pro Val Gln Gly Ser Ser Ser Gly Gly Glu Ala

210 215 220

Arg Ser Pro Ser Ser Asn Gln Ser Leu Gln Glu Arg Gly Ser Gln Glu

225 230 235 240

Asp Leu Asp Ser Ser Gly Thr Lys Lys Asn Glu Asn Ala Cys Arg Ala

245 250 255

Ser Ser Thr Glu Met Glu Asn Phe Ser Lys Lys Leu Asp Ser Ala Glu

260 265 270

Asn Ile Gly Arg Glu Ile Gly Thr Asn Ala Met Glu Asp Met Pro Cys

275 280 285

Val Phe Thr Lys Gly Asp Gly Pro Asn Gly Arg Arg Val Asp Gly Ile

290 295 300

Leu Tyr Lys Tyr Gly Lys Gly Glu Glu Val Arg Ile Met Cys Val Cys

305 310 315 320

His Gly Ser Phe Leu Ser Pro Ala Glu Phe Val Lys His Ala Gly Gly

325 330 335

Ser Asp Val Asn His Pro Leu Arg His Ile Val Ile Asn Ser Ser Gly

340 345 350

Pro Ser Phe Val Val

355

<210>3

<211>23

<212>DNA

<213>Artificial Sequence

<400>3

atgggagaaa caaacgagaa tag 23

<210>4

<211>22

<212>DNA

<213>Artificial Sequence

<400>4

cacaaaggac ggaccagaag ag 22

Claims (10)

1. The populus tomentosa MODD1 gene is characterized in that the nucleotide sequence of the populus tomentosa MODD1 gene is as follows:

i) 1, SEQ ID NO; or

ii) the nucleotide sequence shown in SEQ ID No.1 is substituted, deleted and/or added with one or more nucleotides to encode the nucleotide sequence of the same functional protein.

2. A protein encoded by the Populus tomentosa MODD1 gene of claim 1, wherein the amino acid sequence of the protein is as follows:

1) an amino acid sequence shown as SEQ ID No. 2; or

2) The amino acid sequence of the protein with the same function is obtained by replacing, inserting or deleting one or more amino acids in the amino acid sequence shown in SEQ ID No. 2.

3. A biomaterial comprising the populus tomentosa MODD1 gene of claim 1, wherein the biomaterial is one or more of a vector, a transgenic cell line, an engineered bacterium, a host cell, or an expression cassette.

4. Use of populus tomentosa MODD1 gene or its encoded protein as claimed in claim 1 or biomaterial as claimed in claim 3 for modulating drought resistance of plants.

5. Use of populus tomentosa MODD1 gene or its encoded protein as claimed in claim 1 or biomaterial as claimed in claim 3 in plant genetic breeding or in the preparation of transgenic plants.

6. The use according to claim 4 or 5, wherein the drought resistance of the plant is improved by increasing the expression level of the aspen MODD1 gene;

the method for improving the expression level of the Chinese white poplar MODD1 gene is to overexpress the Chinese white poplar MODD1 gene in the plant.

7. Use of populus tomentosa MODD1 gene or its encoded protein as claimed in claim 1 or biomaterial as claimed in claim 3 for increasing abscisic acid content in plants.

8. A method of modulating drought resistance in a plant comprising: regulating and controlling the expression level of Chinese white poplar MODD1 gene in plant;

the nucleotide sequence of the populus tomentosa MODD1 gene is as follows:

i) 1, SEQ ID NO; or

ii) the nucleotide sequence shown in SEQ ID No.1 is substituted, deleted and/or added with one or more nucleotides to encode the gene of the same functional protein.

9. The method according to claim 8, wherein drought resistance of the plant is increased by increasing the expression level of the aspen MODD1 gene in the plant, preferably by increasing the content of abscisic acid in the plant; alternatively, drought-resistant lines are cultivated by crossing the lines over expressing the populus tomentosa MODD1 gene with other lines.

10. The method as claimed in claim 9, wherein the line overexpressing the populus tomentosa MODD1 gene is constructed by:

the populus tomentosa MODD1 gene is connected to a plant expression vector and is obtained by transforming a wild type strain through agrobacterium GV 3101.

Images