CN111139030A - Enzyme modified biomacromolecule wood adhesive and preparation method thereof - Google Patents
Enzyme modified biomacromolecule wood adhesive and preparation method thereof Download PDFInfo
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- CN111139030A CN111139030A CN202010019622.4A CN202010019622A CN111139030A CN 111139030 A CN111139030 A CN 111139030A CN 202010019622 A CN202010019622 A CN 202010019622A CN 111139030 A CN111139030 A CN 111139030A
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09J—ADHESIVES; NON-MECHANICAL ASPECTS OF ADHESIVE PROCESSES IN GENERAL; ADHESIVE PROCESSES NOT PROVIDED FOR ELSEWHERE; USE OF MATERIALS AS ADHESIVES
- C09J189/00—Adhesives based on proteins; Adhesives based on derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09J—ADHESIVES; NON-MECHANICAL ASPECTS OF ADHESIVE PROCESSES IN GENERAL; ADHESIVE PROCESSES NOT PROVIDED FOR ELSEWHERE; USE OF MATERIALS AS ADHESIVES
- C09J11/00—Features of adhesives not provided for in group C09J9/00, e.g. additives
- C09J11/02—Non-macromolecular additives
- C09J11/04—Non-macromolecular additives inorganic
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09J—ADHESIVES; NON-MECHANICAL ASPECTS OF ADHESIVE PROCESSES IN GENERAL; ADHESIVE PROCESSES NOT PROVIDED FOR ELSEWHERE; USE OF MATERIALS AS ADHESIVES
- C09J11/00—Features of adhesives not provided for in group C09J9/00, e.g. additives
- C09J11/08—Macromolecular additives
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K3/00—Use of inorganic substances as compounding ingredients
- C08K3/18—Oxygen-containing compounds, e.g. metal carbonyls
- C08K3/20—Oxides; Hydroxides
- C08K3/22—Oxides; Hydroxides of metals
- C08K2003/2206—Oxides; Hydroxides of metals of calcium, strontium or barium
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L2205/00—Polymer mixtures characterised by other features
- C08L2205/02—Polymer mixtures characterised by other features containing two or more polymers of the same C08L -group
- C08L2205/025—Polymer mixtures characterised by other features containing two or more polymers of the same C08L -group containing two or more polymers of the same hierarchy C08L, and differing only in parameters such as density, comonomer content, molecular weight, structure
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L2205/00—Polymer mixtures characterised by other features
- C08L2205/03—Polymer mixtures characterised by other features containing three or more polymers in a blend
- C08L2205/035—Polymer mixtures characterised by other features containing three or more polymers in a blend containing four or more polymers in a blend
Abstract
The invention belongs to the technical field of wood adhesives, and particularly relates to an enzyme modified biomacromolecule wood adhesive and a preparation method thereof. The preparation method comprises the following steps: taking vegetable protein, cellulose and lignin, performing ultrasonic treatment to uniformly disperse the vegetable protein, cellulose and lignin in water, adjusting the pH value of the solution to 7-11 by using alkaline hydroxide, and stirring for 2 hours at 50 ℃; adding protease, stirring at 37 deg.C for 3 hr or overnight at 4 deg.C; and (4) homogenizing under high pressure to obtain the wood adhesive. The formula of the soybean-based wood adhesive only contains biomacromolecules such as protein, cellulose, lignin and low-concentration alkali, does not contain formaldehyde, and is green and pollution-free; the protease modification enables three biological macromolecules to interact to form an organic and stable macromolecular composite system, so that the bonding strength and the water resistance of the adhesive are improved, the problem of recycling of crop resources is solved, and the adhesive has certain economic benefit.
Description
Technical Field
The invention belongs to the technical field of wood adhesives, and particularly relates to an enzyme modified biomacromolecule wood adhesive and a preparation method thereof.
Background
The plate has wide application in our life and can not be separated from the adhesive. Adhesives, also known as glues, adhesives, mucilages or pastes, have wide application in human society, and are mainly petrochemical products and natural renewable resources as raw materials; is applied to one or both surfaces of two separate articles to bond them together and to resist their separation.
The natural adhesive has the advantages of poor water resistance, low solid content, low bonding strength, easy mildew and short storage period, and is gradually replaced by the 'three-aldehyde adhesive' which has the advantages of high bonding strength, short curing time, high concentration, low viscosity, easy mixing with water, simple process and convenient operation. However, wood products prepared by the application of the trialdehyde glue release formaldehyde gas, and the harm to human bodies is very great. In 2017, 10 and 27, in a carcinogen list published by the international cancer research institution of the world health organization, formaldehyde is put in a carcinogen list. Formaldehyde is a big killer for human health, the release period of formaldehyde is as long as 5-15 years, and strict formaldehyde release limits in indoor air and wood products are established in many countries, so that the wood industry faces severe challenges in the face of increasingly strict environmental standards.
In recent years, with the increase of international market competition pressure, the environmental protection grade of the artificial boards is continuously improved, and the environmental protection consciousness of global citizens to home life is continuously enhanced, and a plurality of domestic artificial board manufacturers are concerned with the development and popularization of environmental protection products and occupy certain market positions. Although many synthetic resins with low trace formaldehyde emission are being developed, many complex problems arise during the research process. In addition, as the energy crisis of all countries in the world continuously appears, the price of the synthetic resin is continuously increased, the research cost is higher, the product competition is weak, and the manufacturers are difficult to break through the bottleneck of export. Therefore, under various pressures in the present society, wood adhesives modified or modified with renewable materials, particularly with grain-based seed proteins, have become a research hotspot of researchers. There are patents (200710164080.4, 201910064132.3,201910147935.5,201910336245.5) which disclose adhesives based on soybean and generally use propylene oxide, ethylene triamine, glutaraldehyde, polyisocyanate, etc. as a crosslinking agent for soybean protein to increase adhesive strength, but these adhesives are carcinogenic, toxic, flammable, or irritating to eyes and skin, and are contrary to the needs of people and the concept of environmental protection. Therefore, it is a trend to find adhesives using biomass as a crosslinking agent or an auxiliary material for increasing adhesive strength and using vegetable protein as a main material.
Disclosure of Invention
Aiming at the problems that the existing adhesive has large formaldehyde release amount, is harmful to human health, has high manufacturing cost, poor process applicability, no product competitiveness, strong harmfulness of a soybean protein crosslinking agent and the like, the invention provides an enzyme modified biomacromolecule wood adhesive and a preparation method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
an enzyme modified biomacromolecule wood adhesive comprises the following components in percentage by mass: 4-12% of vegetable protein, 0.1-0.8% of alkaline hydroxide, 0.001-0.005% of protease, 1-3% of cellulose, 2-10% of lignin and the balance of water.
Further, the vegetable protein is any one of bean protein, whey protein, broomcorn millet gluten protein, peanut protein, zein, cassava crude protein or a protein mixture; the protein mixture is composed of bean protein and any one of whey protein, broomcorn millet gluten protein, peanut protein, zein and cassava crude protein in a mass ratio of 10-20: 1;
the alkaline hydroxide is IA group alkali metal hydroxide or IIA alkaline earth metal hydroxide;
the protease is protein cross-linking enzyme or direct cross-linking enzyme;
the lignin is a group IA or IIA lignosulfonate.
Still further, the raw material of the bean protein is soybean protein isolate; the alkaline hydroxide is sodium hydroxide or potassium hydroxide or calcium hydroxide; the protease is tyrosinase, laccase, peroxidase or transglutaminase; the lignin is sodium lignosulfonate or potassium lignosulfonate.
A preparation method of an enzyme modified biomacromolecule wood adhesive comprises the steps of respectively taking vegetable protein, cellulose, lignin and water according to the mass percentage of 4-12% of vegetable protein, 0.1-0.8% of alkaline hydroxide, 0.001-0.005% of protease, 1-3% of cellulose, 2-10% of lignin and the balance of water, uniformly dispersing the vegetable protein, the cellulose and the lignin in the water, taking the alkaline hydroxide to adjust the pH value of the solution to 7-11, stirring for 2 hours at 50 ℃ to form a mixed solution of the vegetable protein cellulose and the lignin, then adding the protease, stirring for 3 hours or 4 ℃ overnight at 37 ℃, and homogenizing under high pressure to obtain the enzyme modified biomacromolecule wood adhesive;
or dissolving plant protein in water, adjusting the pH value of the solution to 7-11 by using an alkaline hydroxide, stirring for 2 hours at 50 ℃ to form a plant protein solution, then adding protease, stirring for 3 hours or overnight at 4 ℃ at 37 ℃, then uniformly dispersing cellulose and lignin in the solution, and homogenizing under high pressure to obtain the enzyme-modified biomacromolecule wood adhesive;
or uniformly dispersing the plant protein, the cellulose and the lignin in water, taking the alkaline hydroxide to adjust the pH value of the solution to 7-8, stirring for 2 hours at 50 ℃ to form a mixed solution of the plant protein, the cellulose and the lignin, then adding the protease, stirring for 3 hours or overnight at 4 ℃ at 37 ℃, then continuously adding the alkaline hydroxide to adjust the pH value to 8-11, and homogenizing under high pressure to obtain the enzyme modified biomacromolecule wood adhesive.
Further, the pressure of the high-pressure homogenization is as follows: low pressure 35MPa (5000psi), volume: 100mL, temperature: 4 ℃ is prepared.
And further, the dispersion is ultrasonic dispersion, the ultrasonic working time is 5s, the intermittent time is 5s, the total time is 200s, the power is 350W, the temperature is 4 ℃, and the volume is 100 mL.
Compared with the prior art, the invention has the following advantages:
1. the enzyme modified biomacromolecule wood adhesive disclosed by the invention is composed of vegetable protein, alkaline hydroxide, protease, cellulose, lignin and water, avoids the formaldehyde hazard of the existing trialdehyde wood adhesive, and solves the problem of formaldehyde release from the source.
2. The vegetable protein in the enzyme modified biomacromolecule wood adhesive is a main by-product in the downstream of the large-scale agricultural product processing industry, and belongs to renewable resources. Large and rich, no region limitation, wide source and controllable cost.
3. In order to ensure the stability, particularly the water resistance, of the adhesive, the enzyme-modified biomacromolecule wood adhesive adopts a protease modification technology, soybean protein isolate molecules added with enzyme form a polymer with larger relative molecular mass, and the apparent diameter of the protein molecules is enlarged, so that the protein solubility of the soybean protein isolate molecules is reduced. Meanwhile, the hydroxyl is introduced by adding cellulose, so that the number of hydrogen bonds between the adhesive and the wood is increased; in addition, lignin is added into the adhesive, so that the effect of homogenizing wood is achieved, the bonding strength is increased, and the water resistance is further improved.
4. The formula of the invention has the advantages of environmental protection and no pollution, and the enzyme modified biomacromolecule wood adhesive disclosed by the invention is mainly prepared from the materials which are reused for rural crop resources, is convenient for increasing the income of farmers, and has good social benefits and economic benefits. The preparation process is simple, the problem of exhaustion of petrochemical raw materials used in the plywood is solved, the principles of environment protection and sustainable development are met, and the prepared adhesive is strong in adhesive force and has a very good application prospect.
Detailed Description
Example 1
The invention relates to an enzyme modified biomacromolecule wood adhesive, which comprises the following components in percentage by mass, and each 100 g of the adhesive comprises: 4% of soybean protein isolate, 0.1% of sodium hydroxide, 0.001% of tyrosinase, 1% of cellulose, 2% of sodium lignosulfonate and the balance of water.
The invention relates to a preparation method of an enzyme modified biomacromolecule wood adhesive, which comprises the steps of respectively taking soybean protein isolate, cellulose, sodium lignosulfonate and water, uniformly and ultrasonically dispersing the soybean protein isolate, the cellulose and the sodium lignosulfonate in the water, wherein the ultrasonic working time is 5s, the intermittent time is 5s, the total time is 200s, the power is 350W, the temperature is 4 ℃, and the volume is 100 mL; and adjusting the pH value of the solution to 7 by using sodium hydroxide, stirring the solution at 50 ℃ for 2 hours to form a mixed solution of plant protein cellulose and lignin, adding tyrosinase, stirring the mixed solution at 37 ℃ for 3 hours, and homogenizing the mixed solution at high pressure under the conditions of the pressure of 35MPa (5000psi), the volume of 100mL and the temperature of 4 ℃ to obtain the wood adhesive.
Example 2
The invention relates to an enzyme modified biomacromolecule wood adhesive, which comprises the following components in percentage by mass, and each 100 g of the adhesive comprises: 8% of lactalbumin, 0.3% of potassium hydroxide, 0.004% of laccase, 2% of cellulose, 6% of sodium lignosulfonate and the balance of water.
The invention relates to a preparation method of an enzyme modified biomacromolecule wood adhesive, which comprises the steps of respectively taking whey protein, cellulose, sodium lignin sulfonate and water, uniformly and ultrasonically dispersing the whey protein, the cellulose and the sodium lignin sulfonate in the water, carrying out ultrasonic working for 5s, carrying out intermittent time for 5s, carrying out total time for 200s, carrying out power 350W, carrying out temperature 4 ℃ and carrying out volume 100mL, taking potassium hydroxide to adjust the pH value of a solution to be 9, stirring for 2h at 50 ℃ to form a mixed solution of plant protein cellulose and lignin, then adding laccase, stirring for 3h at 37 ℃, and carrying out high-pressure homogenization under the conditions of pressure of 35MPa (5000psi), volume of 100mL and temperature of 4 ℃ to obtain the wood adhesive.
Example 3
The invention relates to an enzyme modified biomacromolecule wood adhesive, which comprises the following components in percentage by mass, and each 100 g of the adhesive comprises: 12% of soybean protein isolate and husked millet gluten protein, 0.8% of calcium hydroxide, 0.005% of peroxidase, 3% of cellulose, 10% of potassium lignosulfonate and the balance of water, wherein the mass ratio of the mixture is 20: 1.
The invention relates to a preparation method of an enzyme modified biological macromolecular wood adhesive, which comprises the steps of uniformly and ultrasonically dispersing broomcorn millet glutelin, cellulose, potassium lignosulfonate and water in the water, carrying out ultrasonic working for 5s and 5s of intermittent time, wherein the total time is 200s, the power is 350W, the temperature is 4 ℃, and the volume is 100mL, adjusting the pH value of a solution to be 8 by using calcium hydroxide, stirring for 2h at 50 ℃ to form a mixed solution of plant protein cellulose and lignin, adding peroxidase, standing overnight at 4 ℃, continuously adding the calcium hydroxide to adjust the pH value to be 11, and carrying out high-pressure homogenization under the conditions of the pressure of 35MPa (5000psi), the volume of 100mL and the temperature of 4 ℃ to obtain the wood adhesive.
Example 4
The invention relates to an enzyme modified biomacromolecule wood adhesive, which comprises the following components in percentage by mass, and each 100 g of the adhesive comprises: 5 percent of soybean protein isolate and whey protein in a mass ratio of 10:1, 0.1 percent of calcium hydroxide, 0.001 percent of transglutaminase, 3 percent of cellulose, 3 percent of sodium lignosulfonate and the balance of water.
The invention relates to a preparation method of an enzyme modified biomacromolecule wood adhesive, which comprises the steps of respectively taking a mixture formed by soy protein isolate and whey protein, cellulose, sodium lignosulfonate and water, uniformly and ultrasonically dispersing the mixture formed by the soy protein isolate and the whey protein in the water, carrying out ultrasonic working for 5s, carrying out intermittent time for 5s, carrying out total time of 200s, carrying out power of 350W, carrying out ultrasonic working at 4 ℃ and carrying out volume of 100mL, taking calcium hydroxide to adjust the pH value of a solution to 7, stirring at 50 ℃ for 2h to form a mixed solution of vegetable protein cellulose and lignin, then adding transglutaminase, stirring at 37 ℃ for 3h, and carrying out high-pressure homogenization under the conditions of 35MPa (5000psi), 100mL in volume and 4 ℃ to obtain the wood adhesive.
Example 5
The invention relates to an enzyme modified biomacromolecule wood adhesive, which comprises the following components in percentage by mass, and each 100 g of the adhesive comprises: the composition comprises a mixture of 11% of soybean protein isolate and husked millet gluten protein in a mass ratio of 15:1, 0.6% of potassium hydroxide, 0.002% of laccase, 2% of cellulose, 5% of potassium lignosulfonate and the balance of water.
The invention relates to a preparation method of an enzyme modified biological macromolecular wood adhesive, which comprises the steps of respectively taking a mixture consisting of soybean protein isolate and husked millet gluten, cellulose, potassium lignosulfonate and water, uniformly and ultrasonically dispersing the mixture consisting of the soybean protein isolate and the husked millet gluten in the water, carrying out ultrasonic working for 5s, carrying out intermittent time for 5s, carrying out total time for 200s, carrying out power 350W, carrying out temperature 4 ℃ and volume 100mL, taking potassium hydroxide to adjust the pH value of the solution to be 8, stirring for 2h at 50 ℃ to form a mixed solution of vegetable protein cellulose and lignin, then adding laccase, carrying out overnight at 4 ℃, and carrying out high-pressure homogenization under the conditions of pressure of 35MPa (5000psi), volume of 100mL and temperature of 4 ℃ to obtain the wood adhesive.
Example 6
The invention relates to an enzyme modified biomacromolecule wood adhesive, which comprises the following components in percentage by mass, and each 100 g of the adhesive comprises: 12 percent of soybean protein isolate and zein, 0.8 percent of calcium hydroxide, 0.005 percent of peroxidase, 1 percent of cellulose, 9 percent of potassium lignosulfonate and the balance of water, wherein the mass ratio of the soybean protein isolate to the zein is 20: 1.
The invention relates to a preparation method of an enzyme modified biomacromolecule wood adhesive, which comprises the steps of respectively taking a mixture formed by soybean protein isolate and zein, cellulose, potassium lignosulfonate and water, dissolving the mixture formed by the soybean protein isolate and the zein in the water, taking calcium hydroxide to adjust the pH value of the solution to 7, stirring for 2 hours at 50 ℃ to form a plant protein solution, then adding peroxidase, stirring for 3 hours at 37 ℃, continuously adding the calcium hydroxide to adjust the pH value to 11, adding the cellulose and the potassium lignosulfonate, stirring uniformly to disperse in the solution, carrying out ultrasonic working for 5s, carrying out intermittent working for 5s, carrying out total time of 200s, carrying out power of 350W, carrying out temperature of 4 ℃ and carrying out volume of 100mL, and carrying out high-pressure homogenization under the conditions of 35MPa (5000psi), 100mL and 4 ℃ to obtain the wood adhesive.
Comparative example 1
8 g of soy protein isolate is dissolved in 96 g of water and stirred for 2h at 50 ℃ until the protein is completely and evenly distributed in the water, and the solution is directly used as a viscose for the bonding of wood.
Comparative example 2
8 g of soy protein isolate is dissolved in 96 g of water, stirred for 2h at 50 ℃ until the protein is completely and uniformly distributed in the water, and sodium hydroxide (0.05 g) is taken to adjust the pH value of the solution to 7, and the solution is directly used as a viscose for bonding wood.
Comparative examples 1-2 are soy-based mucilages prepared using conventional methods.
Comparative example 1
The urea-formaldehyde resin adhesive commonly used in the manufacture of artificial boards is adopted.
Comparative example 2
The melamine resin adhesive commonly used in the manufacture of artificial boards is adopted.
Adhesive performance evaluation test:
basswood board is sawed according to GB/T9846.7-2004,
size of the sample: 100 mm. times.25 mm. Size application area25mm multiplied by 25mm, the thickness of the veneer is 1.5mm, and the water content is 81.2 percent; the glue coating amount is 480g/m2；
The hot pressing process conditions are as follows: the pressure is 1.0-1.2 MPa, the temperature is 140-150 ℃, and the time is 10 min.
The bonding strength is measured by bonding, solidifying and cooling the basswood board to room temperature, immersing the basswood board in warm water (60 +/-3 ℃) for 3 hours, cooling the basswood board for 10 minutes at the room temperature, and averaging 5 samples in each group.
Plywood bonding strength test: the bonding strength is an important index for evaluating the bonding strength of the adhesive, and the bonding strength of the adhesive is better when the test result is a mean value according to the national standard GB/T14074-
Testing the impregnation stripping performance of the plywood: the dipping and peeling performance is an important index for evaluating the water resistance of the adhesive, the measurement method is based on the national standard GB/T17657-2013 of the people's republic of China, the test result is a mean value, and the smaller the numerical value of the test result is, the better the water resistance of the adhesive is.
Testing the content of free formaldehyde: according to the detection of the national standard GB/T14074-.
The test results are shown in table 1 below:
table 1 performance quality index table of adhesive
| Detecting items | Bonding strength/MPa | Rate of exfoliation by dipping | Free formaldehyde |
| Example 1 | 1.79 | 0 | 0 |
| Example 2 | 1.69 | 0 | 0 |
| Example 3 | 1.65 | 0 | 0 |
| Example 4 | 2.43 | 0 | 0 |
| Example 5 | 2.57 | 0 | 0 |
| Example 6 | 2.67 | 0 | 0 |
| Comparative example 1 | 0.65 | 50％ | 0 |
| Comparative example 2 | 0.96 | 20％ | 0 |
| Comparative example 3 | 0.87 | 30％ | 5.8 |
| Comparative example 4 | 1.14 | 20％ | 0.56 |
Experimental results show that after the soybean meal-based adhesive is modified by enzyme and added with cellulose and lignin, the bonding strength of the prepared plywood is obviously higher than that of the soybean meal adhesive before modification, the bonding strength is more than 1.00MPa according to the detection of II-type plywood, some of the bonding strength reaches 2.67MPa, the standard requirement of the plywood is met, and the reinforcing effect is obvious. Meanwhile, the formaldehyde emission of the modified adhesive is 0, and the modified adhesive is high in water resistance.
The embodiments are described in detail, but the present invention is not limited to the above embodiments and examples, and various changes and modifications within the knowledge of those skilled in the art may be made without departing from the spirit of the present invention, and the changes and modifications fall within the scope of the present invention.
Claims (6)
1. An enzyme modified biomacromolecule wood adhesive is characterized in that: comprises the following components in percentage by mass: 4-12% of vegetable protein, 0.1-0.8% of alkaline hydroxide, 0.001-0.005% of protease, 1-3% of cellulose, 2-10% of lignin and the balance of water.
2. The enzyme-modified biomacromolecule wood adhesive according to claim 1, wherein the adhesive comprises: the vegetable protein is any one of bean protein, whey protein, broomcorn millet gluten protein, peanut protein, zein, cassava crude protein or a protein mixture; the protein mixture is composed of bean protein and any one of whey protein, broomcorn millet gluten protein, peanut protein, zein and cassava crude protein in a mass ratio of 10-20: 1;
the alkaline hydroxide is IA group alkali metal hydroxide or IIA alkaline earth metal hydroxide;
the protease is protein cross-linking enzyme or direct cross-linking enzyme;
the lignin is a group IA or IIA lignosulfonate.
3. The enzyme-modified biomacromolecule wood adhesive according to claim 2, wherein the adhesive comprises: the raw material of the bean protein is soybean protein isolate; the alkaline hydroxide is sodium hydroxide or potassium hydroxide or calcium hydroxide; the protease is tyrosinase, laccase, peroxidase or transglutaminase; the lignin is sodium lignosulfonate or potassium lignosulfonate.
4. A preparation method of enzyme modified biomacromolecule wood adhesive is characterized by comprising the following steps: respectively taking vegetable protein, cellulose, lignin and water according to the mass percentage in claim 1, uniformly dispersing the vegetable protein, the cellulose and the lignin in the water, adjusting the pH value of the solution to 7-11 by taking an alkaline hydroxide, stirring for 2 hours at 50 ℃ to form a mixed solution of the vegetable protein cellulose and the lignin, then adding protease, stirring for 3 hours or overnight at 4 ℃ at 37 ℃, and homogenizing under high pressure to obtain the enzyme-modified biomacromolecule wood adhesive;
or dissolving plant protein in water, adjusting the pH value of the solution to 7-11 by using an alkaline hydroxide, stirring for 2 hours at 50 ℃ to form a plant protein solution, then adding protease, stirring for 3 hours or overnight at 4 ℃ at 37 ℃, then uniformly dispersing cellulose and lignin in the solution, and homogenizing under high pressure to obtain the enzyme-modified biomacromolecule wood adhesive;
or uniformly dispersing the plant protein, the cellulose and the lignin in water, taking the alkaline hydroxide to adjust the pH value of the solution to 7-8, stirring for 2 hours at 50 ℃ to form a mixed solution of the plant protein, the cellulose and the lignin, then adding the protease, stirring for 3 hours or overnight at 4 ℃ at 37 ℃, then continuously adding the alkaline hydroxide to adjust the pH value to 8-11, and homogenizing under high pressure to obtain the enzyme modified biomacromolecule wood adhesive.
5. The preparation method of the enzyme-modified biomacromolecule wood adhesive according to claim 4, wherein the method comprises the following steps: the pressure of the high-pressure homogenization is as follows: low pressure 35MPa, volume: 100mL, temperature: 4 ℃ is prepared.
6. The preparation method of the enzyme-modified biomacromolecule wood adhesive according to claim 4, wherein the method comprises the following steps: the dispersion is ultrasonic dispersion, the ultrasonic working time is 5s, the intermittent time is 5s, the total time is 200s, the power is 350W, the temperature is 4 ℃, and the volume is 100 mL.
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| CN111139030B (en) | 2021-09-28 |
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