CN111117562A - Enzyme modified biomass wood adhesive and preparation method thereof - Google Patents
Enzyme modified biomass wood adhesive and preparation method thereof Download PDFInfo
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- CN111117562A CN111117562A CN202010019620.5A CN202010019620A CN111117562A CN 111117562 A CN111117562 A CN 111117562A CN 202010019620 A CN202010019620 A CN 202010019620A CN 111117562 A CN111117562 A CN 111117562A
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- protein
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09J—ADHESIVES; NON-MECHANICAL ASPECTS OF ADHESIVE PROCESSES IN GENERAL; ADHESIVE PROCESSES NOT PROVIDED FOR ELSEWHERE; USE OF MATERIALS AS ADHESIVES
- C09J189/00—Adhesives based on proteins; Adhesives based on derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09J—ADHESIVES; NON-MECHANICAL ASPECTS OF ADHESIVE PROCESSES IN GENERAL; ADHESIVE PROCESSES NOT PROVIDED FOR ELSEWHERE; USE OF MATERIALS AS ADHESIVES
- C09J11/00—Features of adhesives not provided for in group C09J9/00, e.g. additives
- C09J11/02—Non-macromolecular additives
- C09J11/04—Non-macromolecular additives inorganic
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09J—ADHESIVES; NON-MECHANICAL ASPECTS OF ADHESIVE PROCESSES IN GENERAL; ADHESIVE PROCESSES NOT PROVIDED FOR ELSEWHERE; USE OF MATERIALS AS ADHESIVES
- C09J11/00—Features of adhesives not provided for in group C09J9/00, e.g. additives
- C09J11/02—Non-macromolecular additives
- C09J11/06—Non-macromolecular additives organic
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K3/00—Use of inorganic substances as compounding ingredients
- C08K3/18—Oxygen-containing compounds, e.g. metal carbonyls
- C08K3/20—Oxides; Hydroxides
- C08K3/22—Oxides; Hydroxides of metals
- C08K2003/2206—Oxides; Hydroxides of metals of calcium, strontium or barium
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L2205/00—Polymer mixtures characterised by other features
- C08L2205/02—Polymer mixtures characterised by other features containing two or more polymers of the same C08L -group
- C08L2205/025—Polymer mixtures characterised by other features containing two or more polymers of the same C08L -group containing two or more polymers of the same hierarchy C08L, and differing only in parameters such as density, comonomer content, molecular weight, structure
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L2205/00—Polymer mixtures characterised by other features
- C08L2205/03—Polymer mixtures characterised by other features containing three or more polymers in a blend
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L2205/00—Polymer mixtures characterised by other features
- C08L2205/03—Polymer mixtures characterised by other features containing three or more polymers in a blend
- C08L2205/035—Polymer mixtures characterised by other features containing three or more polymers in a blend containing four or more polymers in a blend
Abstract
The invention belongs to the technical field of wood adhesives, and particularly relates to an enzyme modified biomass wood adhesive and a preparation method thereof. The preparation method comprises the following steps: dissolving vegetable protein in water, adjusting pH to 7-11 with alkaline hydroxide, and stirring at 50 deg.C for 2 hr to obtain vegetable protein solution; adding polysaccharide, and uniformly dispersing in the vegetable protein solution by ultrasonic; adding glucosamine and protease, and stirring at 37 deg.C overnight; and (4) homogenizing under high pressure to obtain the wood adhesive. The formula of the soybean-based wood adhesive only contains protein, polysaccharide, low-concentration weak base and glucosamine, and is free of formaldehyde, green and pollution-free; the protease modification and glucosamine modification technology enables the soybean protein and the polysaccharide to form a uniform, compatible and stable net structure, thereby increasing the bonding strength and the water resistance of the adhesive.
Description
Technical Field
The invention belongs to the technical field of wood adhesives, and particularly relates to an enzyme modified biomass wood adhesive and a preparation method thereof.
Background
The application of the board in our life is very wide, and the artificial boards gradually appear in the visual field of people and can not leave adhesives. Adhesives are applied to one or both surfaces of two separate articles to bond them together and to resist their separation. In the initial stage, most of the used adhesives are traditional formaldehyde adhesives, namely urea-formaldehyde adhesives, phenolic adhesives and melamine formaldehyde adhesives, and because the urea-formaldehyde resins have the advantages of high bonding strength, short curing time, high concentration, low viscosity, easiness in mixing with water, simple process, convenience in operation, lower price and the like compared with other materials, eighty percent of the adhesives existing in the current market are all the formaldehyde adhesives. Certainly, things always have two sides, more and more people are killed by formaldehyde poisoning caused by improper treatment of wood products prepared by applying the formaldehyde-formaldehyde glue, the release period of the wood products reaches 5-15 years, the wood products are a big killer of human health, and the formaldehyde is a chemical substance which is confirmed by world health organization to be toxic, carcinogenic and malformed to human bodies.
In recent years, with the increase of international market competition pressure, the environmental protection grade of the artificial board is continuously improved, the environmental protection consciousness of people is gradually enhanced, and a plurality of domestic artificial board manufacturers have been the first to involve in the development and popularization of environmental protection products and occupy certain market positions. Although many synthetic resins with low trace formaldehyde emission are being developed, many complex problems arise during the research process. In addition, as the energy crisis of all countries in the world continuously appears, the price of the synthetic resin is continuously increased, the research cost is higher, the product competition is weak, and the manufacturers are difficult to break through the bottleneck of export. Therefore, under various pressures in the present society, wood adhesives modified or modified with renewable materials, particularly with grain-based seed proteins, have become a research hotspot of researchers. There are patents (200710164080.4, 201910064132.3, 201910147935.5, 201910336245.5) which disclose adhesives based on soy as a material and use propylene oxide, ethylene triamine, glutaraldehyde, polyisocyanate, etc. as a crosslinking agent for soy protein to increase adhesive strength, but these adhesives are carcinogenic, toxic, flammable, or irritating to eyes and skin, which is contrary to the needs and environmental protection concept. Therefore, it is a trend to find adhesives using biomass as a crosslinking agent or an auxiliary material for increasing adhesive strength and using vegetable protein as a main material.
Disclosure of Invention
Aiming at the problems that the existing adhesive has large formaldehyde release amount, is harmful to human health, has high manufacturing cost, poor process applicability, no product competitiveness and the like, the invention provides an enzyme modified biomass wood adhesive and a preparation method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
an enzyme modified biomass wood adhesive comprises the following components in percentage by mass: 4-12% of vegetable protein, 0.1-0.8% of alkaline hydroxide, 0.001-0.005% of protease, 0.4-1.5% of glucosamine, 1-3% of polysaccharide and the balance of water.
Further, the vegetable protein is any one of bean protein, whey protein, broomcorn millet gluten protein, peanut protein, zein, cassava crude protein or a protein mixture; the protein mixture is composed of bean protein and any one of whey protein, broomcorn millet gluten protein, peanut protein, zein and cassava crude protein in a mass ratio of 10-20: 1;
the alkaline hydroxide is IA group alkali metal hydroxide or IIA alkaline earth metal hydroxide;
the glucosamine is glucosamine sulfate and glucosamine hydrochloride;
the protease is protein cross-linking enzyme or direct cross-linking enzyme;
the polysaccharide is cellulose, glucan, pectin or xylan.
Still further, the raw material of the bean protein is soybean protein isolate; the alkaline hydroxide is sodium hydroxide or potassium hydroxide or calcium hydroxide; the protease is tyrosinase, laccase, peroxidase or transglutaminase; the cellulose is hydroxymethyl cellulose, hydroxyethyl cellulose, cellulose acetate, methyl cellulose or hydroxypropyl cellulose.
A preparation method of an enzyme modified biomass wood adhesive comprises the steps of respectively taking vegetable protein, glucosamine, polysaccharide and water according to the mass percentage of 4-12% of vegetable protein, 0.1-0.8% of alkaline hydroxide, 0.001-0.005% of protease, 0.4-1.5% of glucosamine, 1-3% of polysaccharide and the balance of water, dissolving the vegetable protein in the water, taking the alkaline hydroxide to adjust the pH value of the solution to 7-11, stirring for 2 hours at 50 ℃ to form a vegetable protein solution, adding the glucosamine and the protease, stirring overnight at 37 ℃, then uniformly dispersing the polysaccharide in the solution, and homogenizing under high pressure to obtain the enzyme modified biomass wood adhesive;
or uniformly dispersing the plant protein and the polysaccharide in water, adjusting the pH value of the solution to 7-11 by using an alkaline hydroxide, stirring for 2 hours at 50 ℃ to form a mixed solution of the plant protein and the polysaccharide, then adding glucosamine and protease, stirring for 3 hours at 37 ℃ or overnight at 4 ℃, and homogenizing under high pressure to obtain the enzyme modified biomass wood adhesive;
or uniformly dispersing the plant protein and the polysaccharide in water, adjusting the pH value of the solution to 7-8 by taking an alkaline hydroxide, stirring for 2 hours at 50 ℃ to form a mixed solution of the plant protein and the polysaccharide, then adding glucosamine and protease, stirring for 3 hours or overnight at 4 ℃ at 37 ℃, then continuously adding the alkaline hydroxide to adjust the pH value to 8-11, and homogenizing under high pressure to obtain the enzyme modified biomass wood adhesive.
Further, the pressure of the high-pressure homogenization is as follows: the low pressure is 35MPa, the volume is 100mL, and the temperature is 4 ℃.
Further, the dispersion is ultrasonic dispersion, the ultrasonic working time is 5s, the intermittent time is 5s, the total time is 200s, the power is 350W, the temperature is 4 ℃, and the volume is 100 mL.
Compared with the prior art, the invention has the following advantages:
1. the formula of the enzyme modified biomass wood adhesive disclosed by the invention comprises vegetable protein, alkaline hydroxide, protease, glucosamine, polysaccharide and water, and the problem that formaldehyde is released to harm human health is solved from a production source.
2. In order to ensure the stability, particularly the water resistance, of the adhesive, a protease modification technology is adopted, the soybean protein isolate molecules added with enzyme form a polymer with larger relative molecular mass, and the apparent diameter of the protein molecules is enlarged, so that the protein solubility of the soybean protein isolate molecules is reduced. Meanwhile, the hydroxyl is introduced by adding the polysaccharide, so that the number of hydrogen bonds between the adhesive and the wood is increased; in addition, glucosamine is added into the adhesive, and can be crosslinked with soybean protein under the action of enzyme, so that the bonding strength and the water resistance of the adhesive are improved.
3. The formula of the invention has the advantages of green, environmental protection and no pollution. Meanwhile, the main raw materials of the soybean meal and the polysaccharide are grain crop processing residues, are renewable resources, can solve the problems of resource utilization of grain crop waste and high cost of the biomass adhesive, meet the requirements of environmental protection and sustainable development, and have important economic and social benefits.
Detailed Description
Example 1
The invention relates to an enzyme modified biomass wood adhesive, which comprises the following components in percentage by mass, and each 100 g of the adhesive comprises: 4% of soybean protein isolate, 0.1% of sodium hydroxide, 0.001% of tyrosinase, 0.4% of glucosamine sulfate, 2% of hydroxymethyl cellulose and the balance of water.
The invention relates to a preparation method of an enzyme modified biomass wood adhesive, which comprises the steps of respectively taking soybean protein isolate, glucosamine sulfate, hydroxymethyl cellulose and water, dissolving the soybean protein isolate in the water, taking sodium hydroxide to adjust the pH value of the solution to 7, stirring for 2 hours at 50 ℃ to form a plant protein solution, then adding the glucosamine sulfate and tyrosinase, stirring overnight at 37 ℃, then uniformly dispersing the hydroxymethyl cellulose in the solution, carrying out ultrasonic working for 5s, carrying out intermittent time for 5s, carrying out total time for 200s, carrying out power for 350W, carrying out temperature for 4 ℃ and carrying out volume for 100 mL. And (3) homogenizing under the conditions of the pressure of 35MPa (5000psi), the volume of 100mL and the temperature of 4 ℃ to obtain the wood adhesive.
Example 2
The invention relates to an enzyme modified biomass wood adhesive, which comprises the following components in percentage by mass, and each 100 g of the adhesive comprises: 8% of whey protein, 0.3% of potassium hydroxide, 0.004% of laccase, 1.0% of glucosamine hydrochloride, 2% of hydroxyethyl cellulose and the balance of water.
The invention relates to a preparation method of an enzyme modified biomass wood adhesive, which comprises the steps of respectively taking whey protein, hydroxyethyl cellulose and water, uniformly dispersing the whey protein and the hydroxyethyl cellulose in the water, carrying out ultrasonic operation for 5s, carrying out intermittent operation for 5s, carrying out total time for 200s, carrying out power for 350W, carrying out temperature for 4 ℃ and carrying out volume for 100 mL. And (2) adjusting the pH value of the solution to 9 by taking potassium hydroxide, stirring the solution for 2 hours at 50 ℃ to form a mixed solution of plant protein and cellulose, then adding laccase and glucosamine hydrochloride, stirring the solution for 3 hours at 37 ℃, and homogenizing the solution under high pressure at the pressure of 35MPa (5000psi), the volume of 100mL and the temperature of 4 ℃ to obtain the wood adhesive.
Example 3
The invention relates to an enzyme modified biomass wood adhesive, which comprises the following components in percentage by mass, and each 100 g of the adhesive comprises: 12% of soybean protein isolate and husked millet gluten protein, 0.8% of calcium hydroxide, 0.005% of peroxidase, 1.5% of glucosamine sulfate, 3% of glucan and the balance of water, wherein the mass ratio of the soybean protein isolate to the husked millet gluten protein is 20: 1.
The invention relates to a preparation method of an enzyme modified biomass wood adhesive, which comprises the steps of respectively taking husked millet gluten, glucosamine sulfate, glucan and water, dissolving a mixture of soybean protein and husked millet gluten in the water, taking calcium hydroxide to adjust the pH value of the solution to be 11, stirring for 2 hours at 50 ℃ to form a vegetable protein solution, then adding peroxidase and glucosamine sulfate, stirring overnight at 37 ℃, then uniformly dispersing the glucan in the solution, carrying out ultrasonic working for 5s, carrying out intermittent time for 5s, carrying out total time for 200s, carrying out power for 350W, carrying out temperature for 4 ℃ and carrying out volume for 100 mL. And (3) homogenizing under high pressure under the conditions that the pressure is 35MPa (5000psi), the volume is 100mL and the temperature is 4 ℃ to obtain the wood adhesive.
Example 4
The invention relates to an enzyme modified biomass wood adhesive, which comprises the following components in percentage by mass, and each 100 g of the adhesive comprises: the soybean protein milk comprises a mixture of 4% of soybean protein isolate and whey protein in a mass ratio of 10:1, 0.1% of calcium hydroxide, 0.001% of transglutaminase, 0.8% of glucosamine hydrochloride, 2% of methyl cellulose and the balance of water.
The invention relates to a preparation method of an enzyme modified biomass wood adhesive, which comprises the steps of respectively taking a mixture formed by soy protein isolate and whey protein, methyl cellulose and water, uniformly dispersing the mixture formed by the soy protein isolate and the whey protein and the methyl cellulose in the water, carrying out ultrasonic treatment for 5s, carrying out intermittent treatment for 5s, carrying out total time of 200s, carrying out power of 350W, carrying out temperature of 4 ℃ and volume of 100mL, taking calcium hydroxide to adjust the pH value of a solution to be 7, stirring for 2h at 50 ℃ to form a mixed solution of a plant protein solution and the cellulose, then adding transglutaminase and glucosamine hydrochloride, stirring for 3h at 37 ℃, and carrying out high-pressure homogenization under the conditions of 35MPa (5000psi), 100mL in volume and 4 ℃ to obtain the wood adhesive.
Example 5
The invention relates to an enzyme modified biomass wood adhesive, which comprises the following components in percentage by mass, and each 100 g of the adhesive comprises: the composition comprises a mixture of 11% of soybean protein isolate and husked millet gluten protein in a mass ratio of 15:1, 0.5% of potassium hydroxide, 0.002% of laccase, 1.2% of glucosamine sulfate, 2% of hydroxypropyl cellulose and the balance of water.
The invention relates to a preparation method of an enzyme modified biomass wood adhesive, which comprises the steps of respectively taking a mixture consisting of soybean protein isolate and husked millet gluten, glucosamine sulfate, hydroxypropyl cellulose and water, dissolving the mixture consisting of the soybean protein isolate and the husked millet gluten and the hydroxypropyl cellulose in the water, taking potassium hydroxide to adjust the pH value of the solution to be 8, stirring for 2 hours at 50 ℃ to form a mixed solution of plant protein and cellulose, then adding laccase and the glucosamine sulfate, stirring overnight at 37 ℃, then continuously adding potassium hydroxide to adjust the pH value to 11, carrying out ultrasonic working for 5s, carrying out intermittent time for 5s, wherein the total time is 200s, the power is 350W, the temperature is 4 ℃, and the volume is 100 mL. And (3) homogenizing under high pressure under the conditions that the pressure is 35MPa (5000psi), the volume is 100mL and the temperature is 4 ℃ to obtain the wood adhesive.
Example 6
The invention relates to an enzyme modified biomass wood adhesive, which comprises the following components in percentage by mass, and each 100 g of the adhesive comprises: 12 percent of soybean protein isolate and zein, 0.8 percent of calcium hydroxide, 0.005 percent of peroxidase, 1.4 percent of glucosamine, 1 percent of hydroxymethyl cellulose and the balance of water, wherein the mass ratio of the soybean protein isolate to the zein is 20: 1.
The invention relates to a preparation method of an enzyme modified biomass wood adhesive, which comprises the steps of respectively taking a mixture formed by soy protein isolate and zein and water, uniformly and ultrasonically dispersing the mixture formed by the soy protein isolate and the zein and hydroxymethyl cellulose in the water, wherein the ultrasonic working time is 5s, the intermittent time is 5s, the total time is 200s, the power is 350W, the temperature is 4 ℃, and the volume is 100 mL; and (2) adjusting the pH value of the solution to 11 by taking calcium hydroxide, stirring for 2h at 50 ℃ to form a mixed solution of a vegetable protein solution and cellulose, then adding peroxidase and glucosamine, stirring for 3h at 37 ℃, and homogenizing under high pressure under the conditions of pressure of 35MPa (5000psi), volume of 100mL and temperature of 4 ℃ to obtain the wood adhesive.
Comparative example 1
8 g of soy protein isolate is dissolved in 96 g of water and stirred for 2h at 50 ℃ until the protein is completely and evenly distributed in the water, and the solution is directly used as a viscose for the bonding of wood.
Comparative example 2
8 g of soy protein isolate is dissolved in 96 g of water, stirred for 2h at 50 ℃ until the protein is completely and uniformly distributed in the water, and sodium hydroxide (0.05 g) is taken to adjust the pH value of the solution to be 8, and the solution is directly used as a viscose for bonding wood.
Comparative examples 1-2 are soy-based mucilages prepared using conventional methods.
Comparative example 3
The urea-formaldehyde resin adhesive commonly used in the manufacture of artificial boards is adopted.
Comparative example 4
The melamine resin adhesive commonly used in the manufacture of artificial boards is adopted.
Adhesive performance evaluation test:
a basswood plate is sawed according to GB/T9846.7-2004;
size of the sample: 100mm × 25 mm; the sizing area is 25mm multiplied by 25 mm;
the thickness of the single plate is 1.5 mm; the water content is 81.2%; the glue coating amount is 480g/m2；
The hot pressing process conditions are as follows: the pressure is 1.0-1.2MPa, the temperature is 140-150 ℃, and the time is 10 min.
The bonding strength is measured by bonding, solidifying and cooling the basswood board to room temperature, immersing the basswood board in warm water (60 +/-3 ℃) for 3 hours, cooling the basswood board for 10 minutes at the room temperature, and averaging 5 samples in each group.
Plywood bonding strength test: the bonding strength is an important index for evaluating the bonding strength of the adhesive, and the bonding strength of the adhesive is better when the test result is a mean value according to the national standard GB/T14074-
Testing the impregnation stripping performance of the plywood: the dipping and peeling performance is an important index for evaluating the water resistance of the adhesive, the measurement method is based on the national standard GB/T17657-2013 of the people's republic of China, the test result is a mean value, and the smaller the numerical value of the test result is, the better the water resistance of the adhesive is.
Testing the content of free formaldehyde: according to the detection of national standard GB/14074-.
The test results are shown in table 1 below:
table 1 performance quality index table of adhesive
Experimental results show that after the bean pulp-based adhesive is modified by enzyme and glucosamine, and polysaccharide is added, the bonding strength of the prepared plywood is obviously higher than that of the bean pulp adhesive before modification, the bonding strength is more than 1.00MPa according to the detection of II-type plywood, some of the bonding strength reaches 2.56MPa, the standard requirement of the plywood is met, and the reinforcing effect is obvious. Meanwhile, the formaldehyde emission of the modified adhesive is 0, and the modified adhesive is high in water resistance.
The embodiments are described in detail, but the present invention is not limited to the above embodiments and examples, and various changes and modifications within the knowledge of those skilled in the art may be made without departing from the spirit of the present invention, and the changes and modifications fall within the scope of the present invention.
Claims (6)
1. An enzyme modified biomass wood adhesive is characterized in that: comprises the following components in percentage by mass: 4-12% of vegetable protein, 0.1-0.8% of alkaline hydroxide, 0.001-0.005% of protease, 0.4-1.5% of glucosamine, 1-3% of polysaccharide and the balance of water.
2. The enzyme-modified biomass wood adhesive according to claim 1, wherein:
the vegetable protein is any one of bean protein, whey protein, broomcorn millet gluten protein, peanut protein, zein, cassava crude protein or a protein mixture; the protein mixture is composed of bean protein and any one of whey protein, broomcorn millet gluten protein, peanut protein, zein and cassava crude protein in a mass ratio of 10-20: 1;
the alkaline hydroxide is IA group alkali metal hydroxide or IIA alkaline earth metal hydroxide;
the glucosamine is glucosamine sulfate and glucosamine hydrochloride;
the protease is protein cross-linking enzyme or direct cross-linking enzyme;
the polysaccharide is cellulose, glucan, pectin or xylan.
3. The enzyme-modified biomass wood adhesive according to claim 2, wherein: the raw material of the bean protein is soybean protein isolate; the alkaline hydroxide is sodium hydroxide or potassium hydroxide or calcium hydroxide; the protease is tyrosinase, laccase, peroxidase or transglutaminase; the cellulose is hydroxymethyl cellulose, hydroxyethyl cellulose, cellulose acetate, methyl cellulose or hydroxypropyl cellulose.
4. A preparation method of an enzyme modified biomass wood adhesive is characterized by comprising the following steps: respectively taking vegetable protein, glucosamine, polysaccharide and water according to the mass percentage in claim 1, dissolving the vegetable protein in the water, taking alkali hydroxide to adjust the pH value of the solution to 7-11, stirring for 2h at 50 ℃ to form a vegetable protein solution, adding the glucosamine and protease, stirring overnight at 37 ℃, uniformly dispersing the polysaccharide in the solution, and homogenizing under high pressure to obtain the enzyme modified biomass wood adhesive;
or uniformly dispersing the plant protein and the polysaccharide in water, adjusting the pH value of the solution to 7-11 by using an alkaline hydroxide, stirring for 2 hours at 50 ℃ to form a mixed solution of the plant protein and the polysaccharide, then adding glucosamine and protease, stirring for 3 hours at 37 ℃ or overnight at 4 ℃, and homogenizing under high pressure to obtain the enzyme modified biomass wood adhesive;
or uniformly dispersing the plant protein and the polysaccharide in water, adjusting the pH value of the solution to 7-8 by taking an alkaline hydroxide, stirring for 2 hours at 50 ℃ to form a mixed solution of the plant protein and the polysaccharide, then adding glucosamine and protease, stirring for 3 hours or overnight at 4 ℃ at 37 ℃, then continuously adding the alkaline hydroxide to adjust the pH value to 8-11, and homogenizing under high pressure to obtain the enzyme modified biomass wood adhesive.
5. The preparation method of the enzyme-modified biomass wood adhesive according to claim 4, wherein the preparation method comprises the following steps: the pressure of the high-pressure homogenization is as follows: the low pressure is 35MPa, the volume is 100mL, and the temperature is 4 ℃.
6. The preparation method of the enzyme-modified biomass wood adhesive according to claim 4, wherein the preparation method comprises the following steps: the dispersion is ultrasonic dispersion, the ultrasonic working time is 5s, the intermittent time is 5s, the total time is 200s, the power is 350W, the temperature is 4 ℃, and the volume is 100 mL.
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| CN112143456A (en) * | 2020-08-04 | 2020-12-29 | 南京林业大学 | Preparation method of adhesive based on degradation and recombination of protein and polysaccharide |
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| WO2010057177A2 (en) * | 2008-11-17 | 2010-05-20 | Gel-Del Technologies, Inc. | Protein biomaterial and biocoacervate vessel graft systems and methods of making and using thereof |
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| CN112143456A (en) * | 2020-08-04 | 2020-12-29 | 南京林业大学 | Preparation method of adhesive based on degradation and recombination of protein and polysaccharide |
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