CN111135116A - Cosmetic material composition containing fermented arabinogalactan - Google Patents
Cosmetic material composition containing fermented arabinogalactan Download PDFInfo
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- CN111135116A CN111135116A CN201910489747.0A CN201910489747A CN111135116A CN 111135116 A CN111135116 A CN 111135116A CN 201910489747 A CN201910489747 A CN 201910489747A CN 111135116 A CN111135116 A CN 111135116A
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- arabinogalactan
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- skin
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Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Dermatology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Cosmetics (AREA)
Abstract
The present invention relates to a cosmetic material composition for improving skin wrinkles and skin elasticity, which comprises fermented arabinogalactans as an active ingredient. The composition of the present invention can inhibit the generation of wrinkles on the skin, improve the elasticity of the skin, and shrink pores by increasing the expression of collagen genes, elastin genes, and fibrillin genes.
Description
Technical Field
The present invention relates to a cosmetic material composition (cosmetic composition comprising fermented Arabinogalactan).
Background
The skin is the first barrier of the human body, protects body organs from external stimuli such as ultraviolet rays and pollutants, and plays an important role in maintaining organism homeostasis such as thermoregulation. The skin is generally composed of three layers, the epidermis (epidermis), the dermis (dermis) and the hypodermis (hypodermis), and various internal and external factors cause aging. Skin aging is classified into intrinsic aging (intrinsic aging) due to physiological aging and photo aging (photo aging) due to environmental factors, particularly, due to continuous exposure to ultraviolet rays, and the phenomena of water loss, keratinization, loss of skin elasticity, wrinkle formation, pigmentation, and the like occur.
Skin aging is caused by changes in the composition of dermal Extracellular matrix proteins (endothelial proteins). The dermal extracellular matrix is mainly composed of collagen, and there are proteins such as elastin, fibrillin, fibronectin, and proteoglycan. The protein is synthesized in dermal fibroblasts and functions to maintain the elasticity of the skin and to support the structure. The decrease in the production of extracellular matrix proteins due to the decrease in endogenous aging or photoaging results in a decrease in skin elasticity and promotes the production of wrinkles. Therefore, the increased expression level of these proteins in the skin is an important factor for improving skin wrinkles or elasticity.
Vitamin C, α -tocopherol, retinol and derivatives thereof have been used in cosmetics and pharmaceuticals as wrinkle-improving materials, however, when they are used in combination in a dosage form, there is a disadvantage of deterioration or poor chemical stability.
In order to overcome these disadvantages, in recent years, development of a cosmetic material composition for improving wrinkles using substances from natural materials has been attracting attention, and the composition needs to be developed (korean laid-open patent No. 2015-0138633).
In addition, Arabinogalactan (arabinogalacan) is a polysaccharide composed of arabinose and galactose, and is found in many cell walls of terrestrial higher plants, and many Arabinogalactan extracted from larch and the like are commercially available, as well as many kinds of herbs such as Echinacea (Echinacea purpura), angelicae gigantis radix (angelicae acutiloba), and Curcuma longa (Curcuma longa).
Arabinogalactans are readily soluble in water and have the property of low viscosity at high concentrations. Are used industrially as thickeners and stabilizers, especially as emulsion stabilizers in foods such as stretched noodles, sausages, breads, etc. Arabinogalactans are also widely used as adjuvants in the cosmetic industry. When the arabinogalactan is used in admixture with the inorganic pigment and sunscreen agent, the dispersion of the particles becomes rapid and uniform and a high SPF is imparted. In addition, arabinogalactans also have moisture retention properties, and in dry climates, can prevent the loss of moisture from the skin and help to keep the skin moist.
Disclosure of Invention
Accordingly, the present inventors have conducted studies to develop natural products having the effects of inhibiting skin wrinkles, shrinking skin pores, and improving skin elasticity, and as a result, have developed a composition comprising fermented arabinogalactans as an active ingredient, which has the effect of inhibiting skin wrinkle formation and the effect of improving elasticity reduction, thereby having a pore-shrinking effect.
Accordingly, an object of the present invention is to provide a composition containing fermented arabinogalactans as an active ingredient.
However, the technical problems to be solved by the present invention are not limited to the above-mentioned problems, and other problems not mentioned can be clearly understood by those skilled in the art from the following description.
In order to achieve the above objects of the present invention, the present invention provides a cosmetic material composition for inhibiting skin wrinkles, shrinking skin pores and improving skin elasticity, which comprises fermented arabinogalactans as an effective ingredient.
As an embodiment of the present invention, the fermented arabinogalactan is fermented by inoculating black yeast (Aureobasidium pullulans CXHB-8) into the arabinogalactan and culturing.
As another embodiment of the present invention, the content of the fermented arabinogalactan is 0.001 to 30% by weight, relative to the total weight of the composition.
As another embodiment of the present invention, the fermented arabinogalactan increases the expression of more than one gene selected from the group consisting of a type i collagen gene, an elastin gene, and a fibrillin gene.
As another embodiment of the present invention, the composition is one or more dosage forms selected from the group consisting of skin-refreshing lotions, emulsions and creams.
Effects of the invention
The cosmetic material composition of the present invention can reduce the formation of skin wrinkles itself, improve formed wrinkles, and reduce the size of pores by increasing the elasticity of skin, and can improve skin aging by increasing the expression of type i collagen gene, elastin gene, and fibrillin gene.
Drawings
FIG. 1 is a graph showing the results of Experimental example 1 in which fermented arabinogalactan was confirmed to affect the expression of type I collagen gene in human fibroblasts.
FIG. 2 is a graph showing the results of Experimental example 2 in which fermented arabinogalactan was confirmed to affect elastin gene expression in human fibroblasts.
FIG. 3 is a graph showing the results of Experimental example 3 in which fermented arabinogalactan was confirmed to affect the expression of profilin gene in human fibroblasts.
Detailed Description
The present inventors have found that fermented arabinogalactans have an excellent effect of improving skin aging in the course of searching for various natural products to develop a safe ingredient effective in improving wrinkle formation and elasticity reduction, and thus have completed the present invention.
Accordingly, the present invention provides a cosmetic material composition for inhibiting skin wrinkles, shrinking pores, and improving skin elasticity, which comprises fermented arabinogalactan as an effective ingredient.
The cosmetic composition is effective in improving skin aging caused by exogenous factors or endogenous factors. The exogenous factors refer to various external factors (e.g., ultraviolet rays), and the endogenous factors are also referred to as time factors, mainly factors caused by the lapse of time.
The present invention focuses on the fact that arabinogalactan has a moisturizing effect, and in order to confirm a method for enhancing the effect of arabinogalactan, the arabinogalactan is inoculated with noble mold (noble rot) and subjected to liquid fermentation. As a result of confirming the influence on genes of collagen, elastin and fibrillin involved in skin wrinkles and elasticity by the fermented arabinogalactan, it was confirmed that the gene production promoting effect was superior to that of the unfermented arabinogalactan.
The noble mold is a strain of Aureobasidium, preferably Aureobasidium pullulans CXHB-8(Aureobasidi umpululans CXHB-8). The noble mold of the genus Aureobasidium (Aureobasidium) is readily visible in the environment and is usually present as a pathogenic bacterium of fruits or vegetables. Although the morphology is the same, noble molds differ from contaminated molds of strawberries in the absence of mold odor. Colonies (colony) are gray or black, branch irregularly from septate and long aerial hyphae, and form a large number of conidia in the radial dentate processes of the enlarged terminal cells. It is known that in Europe, noble mold (Aureobasidium pullulans) is used for the production of wine like Staphylococcus aureus (Botrytis cinerea), but has never been used for the fermentation of arabinogalactans.
Arabinogalactan used in the present invention is a polysaccharide composed of arabinose and galactose, and is a component abundantly present in the cell wall of terrestrial higher plants. The arabinogalactan of the present invention may be extracted directly from Echinacea purpurea (Echinacea purpurea), Angelica acutiloba (Angelica acutiloba), Curcuma longa (Curcuma longa), Larix dahurica, or the like, or may be commercially available arabinogalactan, but is not limited thereto.
The preparation method of the fermented arabinogalactan comprises the following steps: a step of obtaining arabinogalactan; and in the arabinogalactanInoculating black yeast (Aureobasidium pullulans CXHB-8) as a noble mold into the polysaccharide and fermenting. Specifically, the step of inoculating the noble mold and fermenting may be performed at 20-35 ℃ for 24 hours to 240 hours, more preferably 72 hours to 144 hours. The amount of bacteria used for the inoculation may be 103～109Preferably 105～107。
In the present invention, the fermented arabinogalactan may be used by concentration or dilution, and the content of the fermented arabinogalactan of the present invention may be 0.001 to 50% by weight, preferably 0.01 to 30% by weight, relative to the total weight of the cosmetic material composition. When the content is less than 0.001 wt%, it is difficult to improve skin aging by improving skin wrinkles and elasticity, and when the content is more than 50 wt%, formulation is difficult and feeling in use is reduced.
In addition, the formulation of the cosmetic material composition may be a toner, an emulsion or a cream. The composition can be in the form of a skin lotion, skin softener, toner, astringent, lotion, milk lotion, moisturizing lotion, nourishing lotion, massage cream, nourishing cream, moisturizing cream, hand cream, foundation lotion, essence, nourishing essence, facial mask, soap, foaming cleanser, face cream, body lotion, or body wash.
Another aspect of the present invention provides a functional cosmetic for inhibiting skin wrinkles, shrinking pores, and improving elasticity, which comprises the above cosmetic material composition.
From the examples of the present invention, it can be confirmed that fermented arabinogalactan has the effects of increasing the production of collagen genes, and increasing the production of elastin and fibrillin genes, thereby having the effects of inhibiting the formation of wrinkles and improving skin elasticity.
Hereinafter, preferred embodiments of the present invention will be described to facilitate understanding of the present invention. However, the following examples are provided only for easier understanding of the present invention, and the contents of the present invention are not limited to the following examples.
Examples
Example 1 inoculation of seedsBacteria
As an inoculum, a previously prepared Aureobasidium pullulans CXHB-8(Aureobasidium pullulans CXHB-8) was inoculated in an amount of 105-107Inoculated and cultured in a thermostat at 28 ℃ for 5 days.
Example 2 fermentation extract of arabinogalactan Using Guinea
A precultured noble mold (Aureobasidium pullulans CXHB-8) was inoculated and the first culture was carried out for 5 days (120 hours). The amount of bacteria used for inoculation was 105-107The incubation was carried out at a constant temperature of 28 ℃.
In order to ferment arabinogalactan, 1g of arabinogalactan was dissolved in 100ml of sterile distilled water and sterilized for use, and only cells of noble mold were recovered and inoculated for use after growth on ISP2 medium.
Experimental example 1 measurement of the Effect of fermented arabinogalactan on the Induction of type I collagen Gene expression
To determine the effect of the fermented arabinogalactans on the induction of type I collagen gene expression, a Human fibroblast cell line (Hs 68, Human dermal fibroblast) was tested under the following conditions.
First, Hs68 fibroblasts were cultured at 3.5X105Dispense into 6-well plates, then 5% CO at 37 ℃2The culture was incubated in the conditioned medium for 24 hours. Then, the medium was removed, DPBS was placed and the plate was washed, and after being replaced with a FBS-free medium, it was divided into a group treated with 100ppm of fermented arabinogalactan, unfermented arabinogalactan, and further cultured for 24 hours. After RNA was isolated from cells of each sample using tricyclazole (RNA iso, DAKARA, Japan), RNA was quantified at 260nm using nanodrop, and cDNA was synthesized in an amplifier using 2. mu.g of each RNA (C1000Thermal Cycler, Bio-Rad, USA).
The real-time polymerase chain reaction was carried out in a real-time PCR machine using a mixture of the synthesized cDNA to which the target gene COL1A1 Primer (Forward Primer): CTCGAGGTGGACACCACCCT (SEQ ID NO: 1), the Reverse Primer (Reverse Primer): CAGCTGGATGGCCACATCGG (SEQ ID NO: 2)) and the cyanine dye SYBR Green (SYBR Green supermix, Applied Biosystems, USA) were added, and the expression level of the COL1A1 gene was finally confirmed, and finally the expression level of the gene was analyzed by correcting β -actin gene (Forward Primer: GGCCATCTCTTGCTCGAAGT (SEQ ID NO: 3), Reverse Primer: GACACCTTCAACACCCCAGC (SEQ ID NO: 4)), and the analysis results are shown in FIG. 1.
As shown in fig. 1, COL1a1 expression was significantly increased in the fermented arabinogalactan-treated group compared to the unfermented arabinogalactan-treated group.
From the above results, it was confirmed that the fermented arabinogalactan of the present invention improves wrinkles generated on the skin, improves elasticity, and reduces pore size.
Experimental example 2 measurement of the Effect of fermented arabinogalactan on the Induction of elastin Gene expression
To determine the effect of fermented arabinogalactans on the induction of elastin gene expression, a Human fibroblast cell line (Human dermal fibroplast, Hs68) was tested according to the following conditions.
First, Hs68 fibroblasts were treated at 3.5x105Dispense into 6-well plates, then 5% CO at 37 ℃2After removing the medium and placing it in a DPBS washing plate, after replacing it with a medium containing no FBS, for 24 hours, the medium was removed and placed in a group of 100ppm of fermented arabinogalactans and unfermented arabinogalactans, and further cultured for 24 hours, after isolating RNA from the cells of each sample using tricyclazole (RNA iso, DAKARA, Japan), after quantifying RNA at 260nm using nanodrop, cDNA (C1000Thermal Cycler, Bio-Rad, USA) was synthesized in an amplifier using 2. mu.g of RNA, respectively, a mixture of a target gene Elastin primer (forward primer): CACCTTGCCCTTGTAGAATCCA (SEQ ID NO: 895), a reverse primer: CCATGACAGGTCAACCAGGTT (SEQ ID NO: 6)) and a cyanine dye SYBR Green (SYBRGreen supermix, Applied Biosystems, USA) was added to the synthesized cDNA, and real-time PCR was performed in a real-time PCR machine, and finally the expression level of Elastin gene was confirmed, and the results of the expression level analysis were corrected by β -expression level analysis, as shown in2, respectively.
As shown in fig. 2, the expression of elastin was significantly increased in the fermented arabinogalactan-treated group compared to the unfermented arabinogalactan-treated group.
From the above results, it was confirmed that the expression of elastin was increased when the composition of the present invention was treated, thereby improving skin elasticity and reducing pore size.
EXAMPLE 3 measurement of the Effect of fermented arabinogalactan on the Induction of fibrillar protein Gene expression
To determine the effect of fermented arabinogalactans on the induction of fibrillar protein gene expression, a Human fibroblast cell line (Human dermal fibroblast, Hs68) was tested according to the following conditions.
First, Hs68 fibroblasts were treated at 3.5x105Dispense into 6-well plates, then 5% CO at 37 ℃2After removing the medium, putting in DPBS and washing the plate, and after replacing with a medium containing no FBS, dividing into groups treating 100ppm of fermented arabinogalactan, unfermented arabinogalactan, and further culturing for 24 hours, after isolating RNA from the cells of each sample using tricyclazole (RNA iso, DAKARA, japan), RNA was quantified with nanodrop at 260nm, and then cDNA (C1000Thermal Cycler, Bio-Rad, usa) was synthesized in an amplifier using 2 μ g of RNA, respectively, a mixture of target gene fibrillin primer (forward primer: AATGTCAGACGAAGCCAGGG (seq id No. 7), reverse primer: GATTTGGTGACGGGGTTCCT (seq id No. 8)) and cyanine dye br Green (SYBR Green supersimix, Applied Biosystems, usa) was added to the synthesized cDNA, polymerase chain reaction was performed in real time (real-time) PCR machine, and finally the degree of expression of fibrillin gene chain reaction was confirmed, and the results of gene expression analysis were corrected by β -act analysis, as shown in fig. 3.
As shown in fig. 3, the expression of profilaggrin in the fermented arabinogalactan-treated group was significantly increased compared to the unfermented arabinogalactan-treated group.
From the above results, it was confirmed that the composition of the present invention, when arabinogalactan is used for fermentation, has excellent effects of improving wrinkles, improving elasticity, and shrinking pores, as compared to the case of non-fermentation.
Formulation example 1 preparation of toner
A toner containing fermented arabinogalactan was prepared by a conventional method according to the composition components and composition ratios shown in table 1 below.
TABLE 1
Formulation example 2 preparation of emulsion
An emulsion containing fermented arabinogalactan was prepared by a conventional method according to the composition components and composition ratios shown in table 2 below.
TABLE 2
Formulation example 3 preparation of cream
Cream containing fermented arabinogalactan was prepared by a conventional method according to the composition components and composition ratios shown in table 3 below.
TABLE 3
| Composition (I) | Content (wt%) |
| Fermentation ofArabinogalactan | 1.0 |
| Glycerol | 7.0 |
| Butanediol | 5.0 |
| Glyceryl stearate/PEG-100 stearate | 2.0 |
| Polysorbate 60 | 0.5 |
| Sorbitol stearate | 0.5 |
| Plant squalane (phytosqualane) | 5.0 |
| Jojoba Oil (Jojoba Oil) | 3.0 |
| Caprylic/capric triglyceride | 10.0 |
| Aloe grease (aloe button) | 3.0 |
| Beeswax (Bees wax) | 1.0 |
| Stearyl alcohol | 0.5 |
| Behenyl alcohol | 0.5 |
| Stearic acid | 0.5 |
| Carboxyvinyl polymer | 0.2 |
| Arginine | 0.3 |
| Polydimethylsiloxane (Dimethicone) | 0.5 |
| Preservative | Micro-scale |
| Perfume | Micro-scale |
| Purified water | Residual amount of |
The above description of the present invention is illustrative of the present invention, and it is easily possible for those skilled in the art to change to other embodiments without changing the technical idea and essential technical features of the present invention. The embodiments described above are therefore exemplary in all respects, and the invention is not limited thereto.
Sequence Listing (sequence list)
<110> kesi meishi Co (Cosmax Co Ltd)
<120> Cosmetic composition comprising fermented Arabinogalactan (Cosmetic composition comprising fermented Arabinogalactan)
<130>OPC19005
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<151>2018-11-02
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<213> Artificial Sequence (Artificial Sequence)
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<223> β -actin forward primer (beta-actin forward primer)
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<213> Artificial Sequence (Artificial Sequence)
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<223> β -actin reverse primer (beta-actin reverse primer)
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<213> Artificial Sequence (Artificial Sequence)
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Claims (5)
1. A cosmetic composition for inhibiting skin wrinkles, shrinking skin pores and improving skin elasticity is provided, wherein,
the composition comprises fermented arabinogalactan as an active ingredient.
2. The cosmetic material composition according to claim 1,
the fermented arabinogalactan is fermented by inoculating black yeast into arabinogalactan and culturing.
3. The cosmetic material composition according to claim 1,
the content of the fermented arabinogalactan is 0.001 to 30% by weight relative to the total weight of the composition.
4. The cosmetic material composition according to claim 1,
the fermented arabinogalactan increases the expression of one or more genes selected from the group consisting of a type i collagen gene, an elastin gene, and a fibrillar protein gene.
5. The cosmetic material composition according to claim 1,
the composition is in the form of one or more selected from the group consisting of a skin toner, an emulsion and a cream.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110268759A1 (en) * | 2008-07-11 | 2011-11-03 | Laboratoires Expanscience | Novel anti-stretch mark active agent, and compositions containing same |
| US20130295036A1 (en) * | 2012-05-05 | 2013-11-07 | Flavitpure, Inc. | Method of using wood extracts in cosmetic and hygiene products |
| KR20180044543A (en) * | 2016-10-24 | 2018-05-03 | 주식회사 코리아나화장품 | Cosmetic composition containing fermentative extract of terminalia ferdinandiana with increased amount of vitamin c fermented by aureobasidium pullulans |
| KR20180045932A (en) * | 2016-10-25 | 2018-05-08 | 코스맥스 주식회사 | Cosmetic composition comprising ginseng fruit Fermentation Product by Aureobasidium pullulans and lactic acid bacteria |
| KR20180088214A (en) * | 2017-01-26 | 2018-08-03 | (주)아모레퍼시픽 | Composition for enhancing immunity comprising Ginseng berry polysaccharide |
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2018
- 2018-11-02 KR KR1020180133878A patent/KR102163159B1/en active IP Right Grant
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110268759A1 (en) * | 2008-07-11 | 2011-11-03 | Laboratoires Expanscience | Novel anti-stretch mark active agent, and compositions containing same |
| US20130295036A1 (en) * | 2012-05-05 | 2013-11-07 | Flavitpure, Inc. | Method of using wood extracts in cosmetic and hygiene products |
| KR20180044543A (en) * | 2016-10-24 | 2018-05-03 | 주식회사 코리아나화장품 | Cosmetic composition containing fermentative extract of terminalia ferdinandiana with increased amount of vitamin c fermented by aureobasidium pullulans |
| KR20180045932A (en) * | 2016-10-25 | 2018-05-08 | 코스맥스 주식회사 | Cosmetic composition comprising ginseng fruit Fermentation Product by Aureobasidium pullulans and lactic acid bacteria |
| KR20180088214A (en) * | 2017-01-26 | 2018-08-03 | (주)아모레퍼시픽 | Composition for enhancing immunity comprising Ginseng berry polysaccharide |
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