CN111132996A - 用于重组蛋白表达的融合标签 - Google Patents

用于重组蛋白表达的融合标签 Download PDF

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Publication number
CN111132996A
CN111132996A CN201880062847.9A CN201880062847A CN111132996A CN 111132996 A CN111132996 A CN 111132996A CN 201880062847 A CN201880062847 A CN 201880062847A CN 111132996 A CN111132996 A CN 111132996A
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China
Prior art keywords
fusion
protein
deep
fusion protein
tag
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Pending
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CN201880062847.9A
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English (en)
Chinese (zh)
Inventor
M·赫克特
S·扎西特斯凯
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Princeton University
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Princeton University
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Publication of CN111132996A publication Critical patent/CN111132996A/zh
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201880062847.9A 2017-07-28 2018-07-27 用于重组蛋白表达的融合标签 Pending CN111132996A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762538505P 2017-07-28 2017-07-28
US62/538,505 2017-07-28
PCT/US2018/044156 WO2019023616A1 (en) 2017-07-28 2018-07-27 FUSION TAGS FOR EXPRESSING RECOMBINANT PROTEINS

Publications (1)

Publication Number Publication Date
CN111132996A true CN111132996A (zh) 2020-05-08

Family

ID=63312460

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201880062847.9A Pending CN111132996A (zh) 2017-07-28 2018-07-27 用于重组蛋白表达的融合标签

Country Status (10)

Country Link
US (1) US12473340B2 (https=)
EP (1) EP3658574A1 (https=)
JP (1) JP2020529221A (https=)
KR (1) KR20200037819A (https=)
CN (1) CN111132996A (https=)
AU (1) AU2018307960B2 (https=)
CA (1) CA3071419A1 (https=)
MX (1) MX2020001058A (https=)
SG (1) SG11202000775SA (https=)
WO (1) WO2019023616A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113388009A (zh) * 2021-05-18 2021-09-14 中国科学院福建物质结构研究所 一种标签蛋白及其编码基因、重组载体与应用

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11202000775SA (en) 2017-07-28 2020-02-27 Univ Princeton Fusion tags for recombinant protein expression
EP3983424A1 (en) * 2019-06-14 2022-04-20 Mytide Therapeutics, Inc. A manufacturing process for peptide and protein production
WO2022094146A1 (en) 2020-10-28 2022-05-05 The Trustees Of Princeton University Methods of converting precursor proteins to mature proteins using kex2 proteases
CN116981680A (zh) * 2021-01-14 2023-10-31 生物催化剂有限责任公司 用于肽生产的组合物和方法
KR102868986B1 (ko) * 2022-09-27 2025-10-15 크리포 주식회사 주요 아미노산이 전하성 및 소수성 아미노산으로 구성된 분산된 His-태그 기반 펩타이드 태그를 포함하여 자가 조립체를 형성하는 융합 단백질 및 이를 이용하여 재조합 단백질을 정제하는 방법

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103031328A (zh) * 2012-11-22 2013-04-10 江南大学 利用一种融合标签提高外源蛋白质的表达量

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101659926A (zh) 1994-06-30 2010-03-03 诺沃奇梅兹有限公司 非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子
EP2084179B1 (en) 2006-11-22 2013-05-22 Novo Nordisk A/S Method for making activated carboxypeptidases
EP2831262B1 (en) 2012-03-29 2018-02-14 Biocon Limited Secretion of functional insulin glargine directly into the culture medium through over expression of kex2p intracellularly
JP6538645B2 (ja) 2013-03-14 2019-07-03 インディアナ ユニバーシティー リサーチ アンド テクノロジー コーポレーションＩｎｄｉａｎａ Ｕｎｉｖｅｒｓｉｔｙ Ｒｅｓｅａｒｃｈ Ａｎｄ Ｔｅｃｈｎｏｌｏｇｙ Ｃｏｒｐｏｒａｔｉｏｎ インスリン‐インクレチン複合物
US10000544B2 (en) 2013-07-31 2018-06-19 Biogenomics Limited Process for production of insulin and insulin analogues
WO2017066441A1 (en) * 2015-10-13 2017-04-20 The Research Foundation For The State University Of New York Cleavable fusion tag for protein overexpression and purification
SG11202000775SA (en) 2017-07-28 2020-02-27 Univ Princeton Fusion tags for recombinant protein expression

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103031328A (zh) * 2012-11-22 2013-04-10 江南大学 利用一种融合标签提高外源蛋白质的表达量

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
AJAMALUDDIN MALIK: "Protein fusion tags for efficient expression and purification of recombinant proteins in the periplasmic space of E. coli" *
SREEJITH RARAN-KURUSSI: "The Ability to Enhance the Solubility of Its Fusion Partners Is an Intrinsic Property of Maltose-Binding Protein but Their Folding Is Either Spontaneous or Chaperone-Mediated" *
YINAN WEI: "Enzyme-like proteins from an unselected library of designed amino acid sequences" *
YINAN WEI: "Stably folded de novo proteins from a designed combinatorial library" *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113388009A (zh) * 2021-05-18 2021-09-14 中国科学院福建物质结构研究所 一种标签蛋白及其编码基因、重组载体与应用
CN113388009B (zh) * 2021-05-18 2022-07-19 中国科学院福建物质结构研究所 一种标签蛋白及其编码基因、重组载体与应用

Also Published As

Publication number Publication date
KR20200037819A (ko) 2020-04-09
MX2020001058A (es) 2020-08-06
US12473340B2 (en) 2025-11-18
WO2019023616A1 (en) 2019-01-31
AU2018307960A1 (en) 2020-02-27
CA3071419A1 (en) 2019-01-31
JP2020529221A (ja) 2020-10-08
SG11202000775SA (en) 2020-02-27
EP3658574A1 (en) 2020-06-03
US20200216512A1 (en) 2020-07-09
AU2018307960B2 (en) 2023-03-23

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Application publication date: 20200508