CA3071419A1 - Fusion tags for recombinant protein expression - Google Patents

Fusion tags for recombinant protein expression Download PDF

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Publication number
CA3071419A1
CA3071419A1 CA3071419A CA3071419A CA3071419A1 CA 3071419 A1 CA3071419 A1 CA 3071419A1 CA 3071419 A CA3071419 A CA 3071419A CA 3071419 A CA3071419 A CA 3071419A CA 3071419 A1 CA3071419 A1 CA 3071419A1
Authority
CA
Canada
Prior art keywords
fusion
protein
deep
fusion protein
insulin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3071419A
Other languages
English (en)
French (fr)
Inventor
Michael Hecht
Shlomo Zarzhitsky
Original Assignee
Princeton University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Princeton University filed Critical Princeton University
Publication of CA3071419A1 publication Critical patent/CA3071419A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA3071419A 2017-07-28 2018-07-27 Fusion tags for recombinant protein expression Pending CA3071419A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762538505P 2017-07-28 2017-07-28
US62/538,505 2017-07-28
PCT/US2018/044156 WO2019023616A1 (en) 2017-07-28 2018-07-27 FUSION TAGS FOR EXPRESSING RECOMBINANT PROTEINS

Publications (1)

Publication Number Publication Date
CA3071419A1 true CA3071419A1 (en) 2019-01-31

Family

ID=63312460

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3071419A Pending CA3071419A1 (en) 2017-07-28 2018-07-27 Fusion tags for recombinant protein expression

Country Status (10)

Country Link
US (1) US12473340B2 (https=)
EP (1) EP3658574A1 (https=)
JP (1) JP2020529221A (https=)
KR (1) KR20200037819A (https=)
CN (1) CN111132996A (https=)
AU (1) AU2018307960B2 (https=)
CA (1) CA3071419A1 (https=)
MX (1) MX2020001058A (https=)
SG (1) SG11202000775SA (https=)
WO (1) WO2019023616A1 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11202000775SA (en) 2017-07-28 2020-02-27 Univ Princeton Fusion tags for recombinant protein expression
EP3983424A1 (en) * 2019-06-14 2022-04-20 Mytide Therapeutics, Inc. A manufacturing process for peptide and protein production
WO2022094146A1 (en) 2020-10-28 2022-05-05 The Trustees Of Princeton University Methods of converting precursor proteins to mature proteins using kex2 proteases
CN116981680A (zh) * 2021-01-14 2023-10-31 生物催化剂有限责任公司 用于肽生产的组合物和方法
CN113388009B (zh) * 2021-05-18 2022-07-19 中国科学院福建物质结构研究所 一种标签蛋白及其编码基因、重组载体与应用
KR102868986B1 (ko) * 2022-09-27 2025-10-15 크리포 주식회사 주요 아미노산이 전하성 및 소수성 아미노산으로 구성된 분산된 His-태그 기반 펩타이드 태그를 포함하여 자가 조립체를 형성하는 융합 단백질 및 이를 이용하여 재조합 단백질을 정제하는 방법

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101659926A (zh) 1994-06-30 2010-03-03 诺沃奇梅兹有限公司 非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子
EP2084179B1 (en) 2006-11-22 2013-05-22 Novo Nordisk A/S Method for making activated carboxypeptidases
EP2831262B1 (en) 2012-03-29 2018-02-14 Biocon Limited Secretion of functional insulin glargine directly into the culture medium through over expression of kex2p intracellularly
CN103031328B (zh) * 2012-11-22 2015-08-05 江南大学 利用一种融合标签提高外源蛋白质的表达量
JP6538645B2 (ja) 2013-03-14 2019-07-03 インディアナ ユニバーシティー リサーチ アンド テクノロジー コーポレーションＩｎｄｉａｎａ Ｕｎｉｖｅｒｓｉｔｙ Ｒｅｓｅａｒｃｈ Ａｎｄ Ｔｅｃｈｎｏｌｏｇｙ Ｃｏｒｐｏｒａｔｉｏｎ インスリン‐インクレチン複合物
US10000544B2 (en) 2013-07-31 2018-06-19 Biogenomics Limited Process for production of insulin and insulin analogues
WO2017066441A1 (en) * 2015-10-13 2017-04-20 The Research Foundation For The State University Of New York Cleavable fusion tag for protein overexpression and purification
SG11202000775SA (en) 2017-07-28 2020-02-27 Univ Princeton Fusion tags for recombinant protein expression

Also Published As

Publication number Publication date
KR20200037819A (ko) 2020-04-09
MX2020001058A (es) 2020-08-06
US12473340B2 (en) 2025-11-18
WO2019023616A1 (en) 2019-01-31
AU2018307960A1 (en) 2020-02-27
CN111132996A (zh) 2020-05-08
JP2020529221A (ja) 2020-10-08
SG11202000775SA (en) 2020-02-27
EP3658574A1 (en) 2020-06-03
US20200216512A1 (en) 2020-07-09
AU2018307960B2 (en) 2023-03-23

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