CN111122422A - High-precision sheath flow detection equipment with feedback and working method thereof - Google Patents
High-precision sheath flow detection equipment with feedback and working method thereof Download PDFInfo
- Publication number
- CN111122422A CN111122422A CN202010036423.4A CN202010036423A CN111122422A CN 111122422 A CN111122422 A CN 111122422A CN 202010036423 A CN202010036423 A CN 202010036423A CN 111122422 A CN111122422 A CN 111122422A
- Authority
- CN
- China
- Prior art keywords
- sample
- bottle
- precision
- sheath
- liquid bottle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 111
- 230000001105 regulatory effect Effects 0.000 claims abstract description 45
- 239000002699 waste material Substances 0.000 claims abstract description 36
- 230000007246 mechanism Effects 0.000 claims description 22
- 230000000149 penetrating effect Effects 0.000 claims description 9
- 230000001360 synchronised effect Effects 0.000 claims description 8
- 238000007789 sealing Methods 0.000 claims description 4
- 230000000087 stabilizing effect Effects 0.000 claims description 3
- 238000003556 assay Methods 0.000 claims 4
- 230000003028 elevating effect Effects 0.000 claims 2
- 238000005259 measurement Methods 0.000 abstract description 2
- 239000002105 nanoparticle Substances 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 90
- 239000012530 fluid Substances 0.000 description 10
- 230000005484 gravity Effects 0.000 description 3
- 230000033001 locomotion Effects 0.000 description 3
- 230000001133 acceleration Effects 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000002572 peristaltic effect Effects 0.000 description 2
- 238000010276 construction Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1425—Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
- G01N15/1409—Handling samples, e.g. injecting samples
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention relates to high-precision sheath flow detection equipment and a working method thereof, wherein the high-precision sheath flow detection equipment comprises a rack, a high-precision pressure regulating device arranged on the rack and a sample pressurizing head communicated with the first end of the high-precision pressure regulating device, the second end of the high-precision pressure reducing device is communicated with a high-precision air source, a liftable sample rack is arranged below the sample pressurizing head on the rack, a sample containing bottle to be detected is arranged on the sample rack, the upper end opening part of the sample containing bottle to be detected can be correspondingly matched and sealed with the sample pressurizing head, a channel for introducing pressure from the high-precision pressure regulating device into the sample containing bottle to be detected is arranged on the sample pressurizing head, and the height of a waste liquid bottle can be regulated in the same way or regulated simultaneously, so that the height difference between the waste liquid bottle and the. The high-precision sheath flow detection equipment is reasonable in design and is beneficial to improving the measurement precision of the biological nanoparticles.
Description
The technical field is as follows:
the invention relates to high-precision low-flow sheath flow detection equipment capable of being stable for a long time and a working method thereof.
Background art:
in order to realize sheath flow movement, the traditional sheath flow detection equipment, such as a flow cytometer, not only can sheath fluid wrap sample flow and move together, but also mostly adopts a probe to extract a sample, and the sample is conveyed by a small peristaltic pump or an injection pump; the sheath liquid provides power for pressure transmission mechanisms such as a peristaltic pump or a diaphragm pump; the disadvantage of this method is that the pressures of the sample flow and the sheath fluid flow vary from moment to moment with the instability of the transmission power, causing instability in the flow rate and flow state, and thus making it difficult to achieve higher accuracy of measurement.
The invention content is as follows:
in view of the defects of the prior art, the technical problem to be solved by the invention is to provide high-precision sheath flow detection equipment and a working method thereof.
The invention discloses high-precision sheath flow detection equipment, which is characterized in that: the sample pressurizing device comprises a rack, a high-precision pressure regulating device arranged on the rack and a sample pressurizing head communicated with the first end of the high-precision pressure regulating device, wherein the second end of the high-precision pressure regulating device is communicated with a high-precision air source, a liftable sample frame is arranged below the sample pressurizing head on the rack, a sample containing bottle to be tested is arranged on the sample frame, the upper port of the sample containing bottle to be tested can be matched and sealed with the sample pressurizing head correspondingly, a channel for leading pressure from the high-precision pressure regulating device into the sample containing bottle to be tested is arranged on the sample pressurizing head in a penetrating manner, a capillary tube capable of extending into the sample containing bottle to be tested is arranged on the sample pressurizing head in a penetrating manner, the upper end of the capillary tube is connected with the lower end of a first pipeline, the upper end of the first pipeline extends into a flowing chamber, the first outlet end of the flowing chamber is connected with a sheath liquid bottle through, the sheath liquid bottle is placed on a sensor mounting panel of liftable height-adjusting, the position of placing the sheath liquid bottle on the sensor mounting panel is equipped with the first mass sensor who is used for measuring sheath liquid bottle quality, be equipped with the second mass sensor who is used for measuring waste liquid bottle quality on the platform face that waste liquid bottle placed, on the same principle, adjust the height of waste liquid bottle, perhaps the two is adjusted simultaneously, and the purpose is the difference in height of guaranteeing the two invariable.
Further, the sensor mounting plate is mounted on a lifting mechanism, and the lifting mechanism is mounted on a lifting frame.
Furthermore, the lifting mechanism is driven by a guide rail screw rod, a synchronous belt wheel or a gear.
Furthermore, valves are connected in series on the second pipeline and the third pipeline.
Furthermore, the liftable sample frame is driven by a cylinder arranged on the frame or driven by a motor screw rod, a synchronous belt and manually lifted.
The invention discloses a working method of high-precision sheath flow detection equipment, which is characterized by comprising the following steps: the device comprises a rack, a high-precision pressure regulating device arranged on the rack and a sample pressurizing head communicated with the first end of the high-precision pressure regulating device, wherein the second end of the high-precision pressure regulating device is communicated with a high-precision air source, a liftable sample frame is arranged below the sample pressurizing head on the rack, a sample containing bottle to be tested is arranged on the sample frame, the upper port of the sample containing bottle to be tested can be matched and sealed with the sample pressurizing head correspondingly, a channel for leading pressure from the high-precision pressure regulating device into the sample containing bottle to be tested is arranged on the sample pressurizing head in a penetrating manner, a capillary tube capable of extending into the sample containing bottle to be tested is arranged on the sample pressurizing head in a penetrating manner, the upper end of the capillary tube is connected with the lower end of a first pipeline, the upper end of the first pipeline extends into a flowing chamber, the first outlet end of the flowing chamber is connected with a sheath liquid bottle through a second, the sheath liquid bottle is placed on a sensor mounting plate capable of adjusting the height in a lifting mode, a first quality sensor used for measuring the quality of the sheath liquid bottle is arranged at the position, where the sheath liquid bottle is placed, on the sensor mounting plate, and a second quality sensor used for measuring the quality of the waste liquid bottle is arranged on a platform surface where the waste liquid bottle is placed;
during working, 1) starting the equipment to open a high-precision air source and reset, lifting the sample frame and driving the sample containing bottle to be tested to ascend to be matched with the sample pressurizing head to finish sealing, and contacting a capillary tube on the sample pressurizing head with a liquid sample in the sample containing bottle to be tested, so that the first step is finished;
2) meanwhile, the sheath liquid bottle or the waste liquid bottle is adjusted in height through the lifting mechanism according to the mass values fed back by the first mass sensor and the second mass sensor;
3) executing an air source to pressurize the sample containing bottle to be detected, opening a pressure regulating device, and stabilizing the pressure of the sample in the sample containing bottle to be detected;
4) the sample in the sample containing bottle to be tested rises into the flowing chamber through the capillary;
5) meanwhile, sheath liquid in the sheath liquid bottle flows into the flow chamber through a second pipeline, the capillary is supported, a flow structure is formed at the same time, and the sheath liquid and the sample are detected in the flow chamber;
6) the detected sample and sheath liquid flow into a waste liquid bottle;
7) the air pressure of the fine adjustment pressure regulating device in the execution process ensures the flow rate of the sample, and the height of the sheath liquid is adjusted to ensure the flow rate of the sheath liquid, so that the sheath liquid is matched with a proper value until the detection is completed.
The invention adopts the main pressure provided by the propulsion of the gas source of the sample to be measured in a sealed state to achieve the effect of balancing the atmospheric pressure, detects the pressure fluctuation by using the high-precision pressure sensor, then regulates the total sample pressure value by the high-precision pressure regulating device, and is matched with the high-precision pressure sensor; the pressure between the sheath liquid bottle and the waste liquid bottle is controlled by adopting gravity generated by the height difference value of the sheath liquid bottle without adopting any pressurizing equipment, and in order to ensure that the pressure is not changed along with the continuous change of the height difference in the using process, the lifting mechanism for automatically adjusting the height of the sheath liquid bottle is arranged and comprises two high-precision mass sensors and a group of lifting mechanisms, the lifting mechanisms can be high-precision guide rail screws or synchronous belts and other similar mechanisms, and the height is constant by adjusting, so that the current technical obstacle is overcome, and the constant flow rate of the sheath liquid bottle is ensured; when the acceleration is needed, the high-precision air source and the lifting mechanism are simultaneously adjusted to achieve the appropriate speed matching of the sample flow and the sheath flow.
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
Description of the drawings:
FIG. 1 is a schematic front view of the present invention;
FIG. 2 is a schematic view of the construction of a waste bottle and its platform surface;
fig. 3 is a schematic cross-sectional view of a sample container to be tested in sealing engagement with a sample pressurizing head.
The specific implementation mode is as follows:
in order to make the aforementioned and other features and advantages of the invention more comprehensible, embodiments accompanied with figures are described in detail below.
The high-precision sheath flow detection device comprises a rack A1, a high-precision pressure regulating device 2 arranged on a rack A1 and a sample pressurizing head 5 communicated with a first end of the high-precision pressure regulating device 2, wherein a second end of the high-precision pressure regulating device 2 is communicated with a high-precision air source 1, a liftable sample rack 6 is arranged below the sample pressurizing head 5 on the rack A1, a sample containing bottle 11 to be detected is arranged on the sample rack 6, an upper port part of the sample containing bottle 11 to be detected can be correspondingly matched and sealed with the sample pressurizing head 5, a channel 12 for leading the pressure from the high-precision pressure regulating device 2 into the sample containing bottle 11 to be detected is arranged on the sample pressurizing head 5, a capillary tube 5001 capable of extending into the sample containing bottle to be detected is arranged on the sample pressurizing head 5 in a penetrating manner, the upper end of the capillary tube 5001 is connected with the lower end of a first pipeline A2, the upper end of the first pipeline A2, flow 4 first exports of room and pass through second pipeline A3 and connect sheath liquid bottle 8, flow 4 second exports of room and pass through third pipeline A4 and connect waste liquid bottle 7, sheath liquid bottle 8 is placed on a liftable height-adjusting's sensor mounting panel 7003, the position of placing the sheath liquid bottle on the sensor mounting panel 7003 is equipped with first mass sensor 7002 who is used for measuring sheath liquid bottle quality, be equipped with the second mass sensor 7001 who is used for measuring waste liquid bottle 7 quality on the platform face that waste liquid bottle 7 placed.
Further, the sensor mounting plate 7003 is mounted on a lifting mechanism 10, and the lifting mechanism 10 is mounted on a lifting frame 9.
Further, the lifting mechanism 9 is driven by other driving means such as a guide screw, a synchronous pulley, or a gear.
Further, the electromagnetic valves 3001 and 3002 are connected in series to the second line A3 and the third line a 4.
Furthermore, the liftable sample frame 6 is driven to lift by the cylinder 3 arranged on the frame, and can also be lifted manually by other modes such as a motor screw, a synchronous belt and the like.
The invention relates to a working method of high-precision sheath flow detection equipment, which comprises a rack, a high-precision pressure regulating device arranged on the rack and a sample pressurizing head communicated with the first end of the high-precision pressure regulating device, wherein the second end of the high-precision pressure regulating device is communicated with a high-precision air source, a liftable sample rack is arranged on the rack and is positioned below the sample pressurizing head, a sample containing bottle to be detected is arranged on the sample rack, the upper port part of the sample containing bottle to be detected can be correspondingly matched and sealed with the sample pressurizing head, a channel for leading pressure from the high-precision pressure regulating device into the sample containing bottle to be detected is arranged on the sample pressurizing head, a capillary tube capable of extending into the sample containing bottle to be detected is arranged on the sample pressurizing head in a penetrating way, the upper end of the capillary tube is connected with the lower end of a first pipeline, the upper end of the first pipeline extends into a flow chamber, the second outlet end of the flowing chamber is connected with a waste liquid bottle through a third pipeline, the sheath liquid bottle is placed on a sensor mounting plate capable of being adjusted in height in a lifting mode, a first quality sensor used for measuring the quality of the sheath liquid bottle is arranged at the position, where the sheath liquid bottle is placed, on the sensor mounting plate, and a second quality sensor used for measuring the quality of the waste liquid bottle is arranged on a platform surface where the waste liquid bottle is placed;
during working, 1) starting the equipment to open a high-precision air source and reset, lifting the sample frame and driving the sample containing bottle to be tested to ascend to be matched with the sample pressurizing head to finish sealing, and contacting a capillary tube on the sample pressurizing head with a liquid sample in the sample containing bottle to be tested, so that the first step is finished;
2) meanwhile, the sheath liquid bottle is adjusted in height through the lifting mechanism according to the mass values fed back by the first mass sensor and the second mass sensor;
3) executing an air source to pressurize the sample containing bottle to be detected, opening a pressure regulating device, and stabilizing the pressure of the sample in the sample containing bottle to be detected;
4) the sample in the sample containing bottle to be tested rises into the flowing chamber through the capillary;
5) meanwhile, sheath liquid in the sheath liquid bottle flows into the flowing chamber through the second pipeline, and supports the capillary to form sheath flow movement at the same time, namely the sheath liquid wraps the sample flow to move together; the sample flow is excited by an excitation light source in the flow chamber to emit light signals, and the light signals are collected by an objective lens and focused to a photoelectric detector, such as a single photon counter, a photomultiplier tube or an avalanche photodiode, for detection.
6) The detected sample and sheath liquid flow into a waste liquid bottle;
7) and in the execution process, the flow speed of the stable sample of the high-precision pressure regulating device is regulated according to the feedback value of the pressure sensor, and the flow speed of the sheath liquid is regulated by regulating the height of the sheath liquid to ensure that the sheath liquid is matched with a proper value until the detection is finished.
The high-precision pressure regulating device can be a model 8286AMBF5 pressure regulating valve produced by PARKER company, the high-precision air source can be a model NMP 850 air pump produced by KNF company or compressed air and the like, the height of the sheath liquid bottle is regulated according to the difference value of the feedback quality values of the first quality sensor and the second quality sensor, and finally the stability of the height difference between the liquid levels of the sheath liquid bottle and the waste liquid bottle is ensured.
The invention adopts the main pressure provided by the propulsion of the gas source of the sample to be tested in a sealed state to achieve the effect of propelling the sample flow, detects the pressure fluctuation by using the high-precision pressure sensor, then regulates the total sample pressure value by the high-precision pressure regulating device, and is matched with the high-precision pressure sensor; the pressure between the sheath liquid bottle and the waste liquid bottle is controlled by adopting gravity generated by the height difference value of the sheath liquid bottle without adopting any pressurizing equipment, and in order to ensure that the pressure is not changed along with the continuous change of the height difference in the using process, the lifting mechanism for automatically adjusting the height of the sheath liquid bottle is arranged and comprises two high-precision mass sensors and a group of lifting mechanisms, the lifting mechanisms can be high-precision guide rail screws or synchronous belts and other similar mechanisms, and the height is constant by adjusting, so that the current technical obstacle is overcome, and the constant flow rate of the sheath liquid bottle is ensured; when the acceleration is needed, the high-precision pressure regulating device and the lifting mechanism are simultaneously regulated to achieve the appropriate speed matching of the sample flow and the sheath flow.
The inventive liquid path system is the core of the instrument, and is characterized in that: first, ultra-low flow: keeping a stable microliter/minute or nanoliter/minute fluid; secondly, ultra-low flow rate: the ultra-slow linear motion control of the flow velocity of a single biological nano particle passing through the laser detection area by 2-20 mm/s can be realized; thirdly, controllability: both the flow rate and the flow velocity can be adjusted within their ranges; fourthly, high stability: the liquid path system of the instrument can keep the state of any flow and flow rate for more than 48 hours, and the deviation is not more than 1%. The instrument provides better resources for the high-precision detection of the modern liquid flow system, and at present, instruments sold and published literature and data and the like cannot achieve the performance of the instrument at home and abroad, so that the instrument is created in the world.
The embodiment only uses the stable sheath fluid pressure and the detection fluid pressure to jointly cooperate to create a stable sheath flow structure, and the method can be also used separately by difference and also belongs to the protection range of the patent; in this row, the source of the stable sheath fluid pressure is obtained by the gravity generated by the height difference of the fluid, and the stable detection fluid pressure is obtained by matching the high-precision air source component with the pressure regulating device. As for the mechanism of sheath liquid or waste liquid altitude mixture control does not influence entire system's detection precision, only provides when running process along with sheath liquid loss or waste liquid increase, guarantees that the liquid level difference is invariable to guarantee that the pressure that sheath liquid flows is invariable for a long time. It is therefore within the scope of this patent to generate a steady pressure differential by any other means that maintains a constant liquid level.
According to the same principle, the mode of constant height difference can be changed into the following forms: the first method comprises the following steps: by increasing or decreasing the liquid in the sheath fluid or waste fluid bottle; for example by pumping away or by adding additional liquid; and the second method comprises the following steps: the pressure brought by the liquid level difference in the sheath liquid bottle or the waste liquid bottle is additionally increased. For example, a stable weight is added, or a plunger is used for providing pressure and the like to increase the liquid pressure in the sheath liquid bottle in the initial state or reduce the liquid pressure in the waste liquid bottle; the above all belong to the protection scope.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention and not to limit it; although the present invention has been described in detail with reference to preferred embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention; without departing from the spirit of the present invention, it is intended to cover all aspects of the invention as defined by the appended claims.
Claims (6)
1. A high accuracy sheath flow check out test set which characterized in that: the sample pressurizing device comprises a rack, a high-precision pressure regulating device arranged on the rack and a sample pressurizing head communicated with the first end of the high-precision pressure regulating device, wherein the second end of the high-precision pressure regulating device is communicated with a high-precision air source, a liftable sample frame is arranged below the sample pressurizing head on the rack, a sample containing bottle to be tested is arranged on the sample frame, the upper port of the sample containing bottle to be tested can be matched and sealed with the sample pressurizing head correspondingly, a channel for leading pressure from the high-precision pressure regulating device into the sample containing bottle to be tested is arranged on the sample pressurizing head in a penetrating manner, a capillary tube capable of extending into the sample containing bottle to be tested is arranged on the sample pressurizing head in a penetrating manner, the upper end of the capillary tube is connected with the lower end of a first pipeline, the upper end of the first pipeline extends into a flowing chamber, the first outlet end of the flowing chamber is connected with a sheath liquid bottle through, the sheath liquid bottle is placed on a sensor mounting panel of liftable height-adjusting, the position of placing the sheath liquid bottle on the sensor mounting panel is equipped with the first mass sensor who is used for measuring sheath liquid bottle quality, be equipped with the second mass sensor who is used for measuring waste liquid bottle quality on the platform face that waste liquid bottle placed, on the same principle, adjust the height of waste liquid bottle, perhaps the two is adjusted simultaneously, and the purpose is the difference in height of guaranteeing the two invariable.
2. The high accuracy sheath flow assay device of claim 1, wherein: the sensor mounting panel is installed on elevating system, elevating system installs on the crane.
3. The high accuracy sheath flow assay device of claim 2, wherein: the lifting mechanism is driven by a guide rail screw rod, a synchronous belt pulley or a gear.
4. The high accuracy sheath flow assay device of claim 1, wherein: and the second pipeline and the third pipeline are connected in series with electromagnetic valves.
5. The high accuracy sheath flow assay device of claim 1, wherein: the liftable sample frame is driven to lift by an air cylinder arranged on the frame, or a motor lead screw, a synchronous belt and a manual lift.
6. A working method of high-precision sheath flow detection equipment is characterized by comprising the following steps: the device comprises a rack, a high-precision pressure regulating device arranged on the rack and a sample pressurizing head communicated with the first end of the high-precision pressure reducing device, wherein the second end of the high-precision pressure reducing device is communicated with a high-precision air source, a liftable sample frame is arranged below the sample pressurizing head on the rack, a sample containing bottle to be tested is arranged on the sample frame, the upper port of the sample containing bottle to be tested can be correspondingly matched and sealed with the sample pressurizing head, a channel for leading pressure from the high-precision pressure reducing device into the sample containing bottle to be tested is arranged on the sample pressurizing head, a capillary tube capable of extending into the sample containing bottle to be tested is arranged on the sample pressurizing head in a penetrating mode, the upper end of the capillary tube is connected with the lower end of a first pipeline, the upper end of the first pipeline extends into a flowing chamber, the first outlet end of the flowing chamber is connected with a sheath liquid bottle through a second pipeline, and the, the sheath liquid bottle is placed on a sensor mounting plate capable of adjusting the height in a lifting mode, a first quality sensor used for measuring the quality of the sheath liquid bottle is arranged at the position, where the sheath liquid bottle is placed, on the sensor mounting plate, and a second quality sensor used for measuring the quality of the waste liquid bottle is arranged on a platform surface where the waste liquid bottle is placed; in the same way, the height of the waste liquid bottle is adjusted, or the waste liquid bottle and the waste liquid bottle are adjusted at the same time, so that the height difference between the waste liquid bottle and the waste liquid bottle is constant;
during working, 1) starting the equipment to open a high-precision air source and reset, lifting the sample frame and driving the sample containing bottle to be tested to ascend to be matched with the sample pressurizing head to finish sealing, and contacting a capillary tube on the sample pressurizing head with a liquid sample in the sample containing bottle to be tested, so that the first step is finished;
2) meanwhile, the sheath liquid bottle is adjusted in height through the lifting mechanism according to the mass values fed back by the first mass sensor and the second mass sensor;
3) executing an air source to start pressurizing the sample containing bottle to be detected, opening a high-precision pressure regulating device, and stabilizing the pressure of the sample in the sample containing bottle to be detected;
4) the sample in the sample containing bottle to be tested rises into the flowing chamber through the capillary;
5) meanwhile, sheath liquid in the sheath liquid bottle flows into the flowing chamber through the second pipeline, the capillary is supported, a sheath flow structure is formed at the same time, and a sample is wrapped by the sheath liquid and enters the flowing chamber to be detected in the flowing chamber;
6) the detected sample and sheath liquid flow into a waste liquid bottle;
7) and the air pressure of the high-precision pressure regulating device is finely regulated in the execution process to ensure the flow velocity of the sample, and the height of the sheath liquid is regulated to ensure the flow velocity of the sheath liquid, so that the sheath liquid is matched with a proper value until the detection is finished.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010036423.4A CN111122422A (en) | 2020-01-14 | 2020-01-14 | High-precision sheath flow detection equipment with feedback and working method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010036423.4A CN111122422A (en) | 2020-01-14 | 2020-01-14 | High-precision sheath flow detection equipment with feedback and working method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111122422A true CN111122422A (en) | 2020-05-08 |
Family
ID=70490416
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010036423.4A Pending CN111122422A (en) | 2020-01-14 | 2020-01-14 | High-precision sheath flow detection equipment with feedback and working method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111122422A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4070617A (en) * | 1974-05-08 | 1978-01-24 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Device for controlling the particle flow in an apparatus for measuring the properties of particles suspended in liquid |
| US4503385A (en) * | 1983-07-11 | 1985-03-05 | Becton, Dickinson And Company | Apparatus and method for regulating sheath fluid flow in a hydrodynamically focused fluid flow system |
| CN101173887A (en) * | 2006-11-02 | 2008-05-07 | 深圳迈瑞生物医疗电子股份有限公司 | Particle analyzer of sheath-flow impedance method |
| CN202734911U (en) * | 2012-06-26 | 2013-02-13 | 深圳市润天智数字设备股份有限公司 | Liquid level measuring device using liquid weight detection |
| CN104493122A (en) * | 2014-12-05 | 2015-04-08 | 华南理工大学 | Semi-continuous casting method and device for gas pressure mould filling |
| CN106872340A (en) * | 2017-02-27 | 2017-06-20 | 赛雷纳（中国）医疗科技有限公司 | A kind of liquid fluid system for FCM analysis |
| CN110234442A (en) * | 2016-11-19 | 2019-09-13 | 厦泰生物科技公司 | Flow cytometer system with stepping flow control valve |
| JP2019178900A (en) * | 2018-03-30 | 2019-10-17 | シスメックス株式会社 | Flow cytometer and method for detecting particles |
-
2020
- 2020-01-14 CN CN202010036423.4A patent/CN111122422A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4070617A (en) * | 1974-05-08 | 1978-01-24 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Device for controlling the particle flow in an apparatus for measuring the properties of particles suspended in liquid |
| US4503385A (en) * | 1983-07-11 | 1985-03-05 | Becton, Dickinson And Company | Apparatus and method for regulating sheath fluid flow in a hydrodynamically focused fluid flow system |
| CN101173887A (en) * | 2006-11-02 | 2008-05-07 | 深圳迈瑞生物医疗电子股份有限公司 | Particle analyzer of sheath-flow impedance method |
| CN202734911U (en) * | 2012-06-26 | 2013-02-13 | 深圳市润天智数字设备股份有限公司 | Liquid level measuring device using liquid weight detection |
| CN104493122A (en) * | 2014-12-05 | 2015-04-08 | 华南理工大学 | Semi-continuous casting method and device for gas pressure mould filling |
| CN110234442A (en) * | 2016-11-19 | 2019-09-13 | 厦泰生物科技公司 | Flow cytometer system with stepping flow control valve |
| CN106872340A (en) * | 2017-02-27 | 2017-06-20 | 赛雷纳（中国）医疗科技有限公司 | A kind of liquid fluid system for FCM analysis |
| JP2019178900A (en) * | 2018-03-30 | 2019-10-17 | シスメックス株式会社 | Flow cytometer and method for detecting particles |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2633283B1 (en) | Dual feedback vacuum fluidics for a flow-type particle analyzer | |
| CN104749085B (en) | Particle analyzer liquid fluid system and particle analyzer | |
| US11781104B2 (en) | Ultra-microinjection detection and control device based on lensless imaging and method thereof | |
| CN111122422A (en) | High-precision sheath flow detection equipment with feedback and working method thereof | |
| CN201233339Y (en) | Sampling apparatus for atmospheric particulate matter | |
| US10613016B2 (en) | Particle analyzing apparatus using a measured pressure of an inner space of a syringe device and a stored standard pressure to correct a particle analysis value | |
| CN208653795U (en) | Electric pushrod test equipment | |
| CN208537364U (en) | A kind of flow cytometer liquid fluid system | |
| CN111075984B (en) | Zero-calibration air-fuel proportional electromagnetic valve | |
| US3262313A (en) | Pressure chamber level gauge with bubble supply | |
| CN115166010A (en) | Flow self-adaptive intelligent gas calibration control method | |
| CN116007716A (en) | Measuring device and measuring method for high-stability tiny liquid flow source | |
| CN105842138B (en) | A kind of mercury column interface automatic regulating system and rock core pore structure analyzer | |
| CN108554207B (en) | Low-concentration standard gas continuous generation device and generation method thereof | |
| CN110479130B (en) | Automatic gas-liquid control fluid proportioning device and fluid mixing system | |
| CN218629675U (en) | Novel gas chromatography air inlet dilution device | |
| CN210426718U (en) | Connecting device for detecting diaphragm gas meter | |
| CN219232696U (en) | Synchronous flotation test system | |
| CN219084874U (en) | Dynamic gas dilution device for malodor determination and malodor dynamic sniffing instrument | |
| CN216900068U (en) | Pneumatic balance degree adjusting system of buoyancy density measuring device | |
| CN208568522U (en) | A kind of device carrying out on-line measurement to insulating oil air content in vacuum oil-filter | |
| CN112304584B (en) | Test method | |
| CN221803062U (en) | Digital metering calibration device for gas flowmeter | |
| JPH01118747A (en) | Particle analyzer | |
| CN117054295A (en) | Device and method for testing dynamic surface tension by bubble pressure method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200508 |
|
| RJ01 | Rejection of invention patent application after publication |