CN208537364U - A kind of flow cytometer liquid fluid system - Google Patents
A kind of flow cytometer liquid fluid system Download PDFInfo
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- CN208537364U CN208537364U CN201721706400.XU CN201721706400U CN208537364U CN 208537364 U CN208537364 U CN 208537364U CN 201721706400 U CN201721706400 U CN 201721706400U CN 208537364 U CN208537364 U CN 208537364U
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Abstract
The utility model discloses a kind of flow cytometer liquid fluid system comprising sample liquid channel, sheath fluid channel, negative pressure source channels and flow chamber；Sample liquid channel, sheath fluid channel, negative pressure source channels are connect with flow chamber；Sheath fluid channel is provided with position potential energy stabilising arrangement, and position potential energy stabilising arrangement includes sheath liquid container, sheath fluid buffer storage, the first sheath fluid circulating pump, the second sheath fluid circulating pump；Sheath fluid buffer storage has a sheath fluid entrance, two sheath fluid outlets；The entrance and sheath fluid reservoir of first sheath fluid circulating pump, outlet are connected to the sheath fluid entrance of sheath fluid buffer storage；The sheath fluid outlet of sheath fluid buffer storage is connected to flow chamber, the sheath fluid outlet of sheath fluid buffer storage is connected to the entrance of the second sheath fluid circulating pump, the outlet of second sheath fluid circulating pump and sheath fluid reservoir, and the sheath fluid outlet being connected in sheath fluid buffer storage with the second sheath fluid circulating pump is higher than its sheath fluid outlet being connected with flow chamber.This system is accurate to sheath fluid control, has prevented sheath fluid pulsation.
Description
Technical field
The utility model belongs to technical field of analytical instruments, is related to biological detection, particle detections, cell analysis technology, tool
Body is related to a kind of flow cytometer liquid fluid system.
Background technique
Flow cytometer generally comprises liquid fluid system, optical system, electronics and signal processing system.Its principle are as follows: will be by
The cell to be measured (or particle) of label is prepared into unicellular (or particle) suspension, as sample liquid.Work of the sample liquid in liquid fluid system
It under, is wrapped up by sheath fluid, focusing forms laminar flow, and stable flows through laser focal beam spot and be inspired fluorescence.Fluorescence quilt
Detection system receives and makees further data analysis, to obtain the information of sample.
From above-mentioned principle it is found that liquid fluid system is the accurate key factor of instrument stabilizer.If focal width is unstable, can lead
Cause cannot form stable unicellular stream, to detecting error occur；If focusing speed is unstable, signal sampling and place will lead to
There is error in reason, influences distribution of results.
There are mainly three types of forms for the power source of liquid fluid system at present: syringe pump, positive pressure expulsion and negative pressure are twitched.Syringe pump by
In inconvenient for use uncommon.The power source of positive pressure expulsion mainly has peristaltic pump, vacuum pump or similar vacuum pump apparatus at present
Three kinds, liquid stream control can be well realized in positive pressure expulsion, if but use peristaltic pump as ahead power source, will lead to liquid flow
It pulses during dynamic, it is very unfavorable that signal is analyzed.Especially with the flow cytometer of sorting function, when detecting mesh
After marking cell, need in the downstream position of runner to target cell capture (such as formation drop), but pulsation phenomenon can usually be led
Cause capture failure.If using vacuum pump or similar device as ahead power source, sheath fluid and (or) sample liquid is needed to be in seal shape
State.Under this state, the effect meeting of pressure is so that a large amount of gas dissolves in sheath fluid and (or) sample liquid.And these are dissolved with the liquid of gas
Body, due to the change of pressure, can release gas to form bubble during flowing in the duct.These bubbles can be led
Meeting is so that instrument cisco unity malfunction under cause analysis error in data or even serious conditions.In addition seal request will lead to equipment
Volume is excessive, increases the complexity of instrument and the consumables cost of user.
The liquid fluid system that negative pressure is twitched, the control of control, sheath fluid stability including negative pressure and sample liquid exposing condition
Feedback control.The negative-pressure power system of liquid fluid system generally comprises more than two aggressive devices at present, such as total negative pressure and sheath fluid
Negative pressure (or positive pressure), to be controlled respectively each runner, while introducing pressure feedback, and that realizes power stablizes output.
In terms of sheath fluid stability, experimental results demonstrate stability has a great impact to the performance of instrument, and quite
It is sensitive.In disclosed CN104749085A patent application, a total active negative pressure device and an active sheath fluid negative pressure have been used
Device.Wherein sheath fluid negative pressure driven devices are used to drive sheath fluid, to obtain relatively stable sheath flow quantity.However, due to sheath
The liquid level of liquid is reduced with using, and position potential energy is continually changing, therefore the technical solution is still difficult
To stable sheath flow quantity.
If above-mentioned sheath fluid negative pressure driven devices carry out continuing to change pressure in use, this change can be compensated
Change.But due in actual use, can not accurately obtain the consumption of sheath fluid, therefore this compensation way is more thick
It is rough, in addition can only gradient compensation.For example the patent application US20040031521A of U.S. Publication describes a kind of holding sheath
The method of liquid liquid level.It installs a liquid level sensor to detect the liquid level of sheath fluid, when height drops in sheath fluid bottle
When low, sheath fluid is supplemented into sheath fluid bottle come transfer tube by the feedback data of sensor, to keep the position potential energy of sheath fluid not
Become, therefore also there is no need to the lasting pressure for changing sheath fluid negative pressure device.However this is perfect condition, in real process, such as
Upper described, due to the corresponding hysteresis quality of the limitation and device of sensor accuracy and sensitivity, this compensation way is simultaneously not allowed
Really, and it is likely to result in sheath fluid pulsation.Device addition excessive simultaneously also reduces system stability, and it is negative to increase instrument
Load.
The control and feedback that sample liquid focuses, including absolute counting, condition monitoring, flow are adjusted, and the side such as cleaning unimpeded
Face.Absolute counting can be used peristaltic pump pumps sample liquid or be detected using flow sensor.I.e. using compacted in CN103900944
Dynamic pump sample introduction.As previously mentioned, peristaltic pump has pulsation effect, and its internal sample carryover amount is also a problem.Compare and
Speech, is fed back, and pass through power device using the other sensors (such as B/P EGR Back Pressure Transducer EGR) of flow sensor coupled system
Control is the desirable selection of comparison.
Aspect is adjusted for sample liquid flow, can not achieve " while changing sample liquid flow, keep sample liquid in the prior art
Focal width it is constant ".Such as adjusting method of disclosed Chinese patent CN104749085A, when sample liquid flow gear is high, normal, basic
When third gear switches, focal width is all different, to can change the unicellular state flowed through, causes the inaccurate of testing result
Really.The feedback and control mode that sample liquid focuses are different with the difference of liquid fluid system principle and layout, but on the whole, existing at present
There is scheme not ensure that focus state is invariable.
In a kind of liquid fluid system for flow cytometry application No. is CN201610655002.3, sheath liquid container and
Sample container is easy for installation without closed.But above-mentioned liquid fluid system is still not carried out the control to sheath fluid stability, and sample liquid is poly-
The feedback control of coke-like condition does not solve influence of the replacement different stalls to focal width, and then the influence to experiment accuracy.
Summary of the invention
In order to solve the above-mentioned technical problem, the utility model provides a kind of novel flow cytometer liquid fluid system, passes through
Sheath fluid position potential energy stabilising arrangement realizes the accurate control to sheath fluid, while not needing the pressure for adjusting sheath fluid runner in real time,
Sheath fluid pulsation phenomenon is prevented；It is realized by feedback to the monitoring of sample liquid focus state and fine-tuning for focal width.
In addition the utility model also helps cleaning and unimpeded, and succinct efficient.High integration substantially reduces the body of liquid fluid system
Product, power consumption and noise, and then also reduce the manufacturing cost and use cost of liquid fluid system.The utility model can also be realized automatic continuous
Add sheath fluid and automatic-discharging waste liquid, operation process is efficient.
A variety of three-way magnetic valves have been used in the utility model, for ease of description, do following agreement herein:
1. all three-way magnetic valves ignore the property of its " One In and Two Out " or " Two In and One Out ", it is only showed only as one
Valve-closed port, a normally opened valve port and a public valve port；
2. valve-closed port is indicated with " B ", normally opened valve port is indicated with " O ", and public valve port is indicated with " P ".
The technical solution of the utility model is as follows:
A kind of flow cytometer liquid fluid system comprising sample liquid channel, sheath fluid channel, negative pressure source channels and flow chamber, institute
State sample liquid channel, sheath fluid channel, negative pressure source channels are connect with the flow chamber；It is characterized in that, the sheath fluid channel setting
Have a position potential energy stabilising arrangement, the position potential energy stabilising arrangement include the sheath liquid container for containing sheath fluid, sheath fluid buffer storage,
First sheath fluid circulating pump, the second sheath fluid circulating pump；The sheath fluid buffer storage has at least one sheath fluid entrance, at least two sheaths
Liquid outlet；The entrance of the first sheath fluid circulating pump and the sheath fluid reservoir, the outlet of the first sheath fluid circulating pump with
The sheath fluid entrance of the sheath fluid buffer storage is connected to；The outlet of at least one sheath fluid and the flow chamber of the sheath fluid buffer storage
At least one sheath fluid outlet of connection, the sheath fluid buffer storage is connected to the entrance of the second sheath fluid circulating pump, and described the
The outlet of two sheath fluid circulating pumps and the sheath fluid reservoir, and the sheath being connected in sheath fluid buffer storage with the second sheath fluid circulating pump
Liquid outlet is higher than its sheath fluid outlet being connected with flow chamber, while flowing to the sheath fluid caching dress through the first sheath fluid circulating pump
The sheath flow quantity set is more than or equal to the sheath flow quantity that the sheath fluid buffer storage flows to the flow chamber.
Preferred technical solution, the sheath fluid buffer storage are the container with single chamber, sheath fluid entrance and sheath fluid
Outlet is in the same container.
Preferred technical solution, the sheath fluid buffer storage are linker, and the sheath fluid buffer storage is with multiple chambers
The container of room, the sheath fluid entrance of the sheath fluid buffer storage are disposed therein on a chamber, and the sheath fluid of sheath fluid buffer storage goes out
Mouth is arranged on other chambers.
Preferred technical solution, the sheath fluid buffer storage can be accurate relative to the height of sample liquid sample introduction needle bottom
It adjusts, to realize the precise controlling to fluid focus, the sheath fluid buffer storage blow-by is preferably by an air mistake
Filter is communicated with the atmosphere, to guarantee that sheath fluid is clean.
Preferred technical solution, be also successively arranged between the sheath fluid buffer storage and the flow chamber flow sensor,
Sheath fluid adjuster, sheath liquid filter, check (non-return) valve.
Preferred technical solution, the negative pressure source channels include negative-pressure power system, waste fluid container, B/P EGR Back Pressure Transducer EGR and two
Two three-way electromagnetic valves of position；The B/P EGR Back Pressure Transducer EGR is connected with waste fluid container and negative-pressure power system composition closed loop, to adjust waste liquid appearance
Pressure in device；One end of the 2/2-way solenoid valve is connected with flow chamber, and the other end is connected to the waste fluid container；It is described
Negative-pressure power system includes vacuum pump, the first three-way magnetic valve, the second three-way magnetic valve；First three-way magnetic valve and described
Second three-way magnetic valve is in parallel, and the normally closed port of the normally opened valve port and the second three-way magnetic valve of first three-way magnetic valve is equal
It is connected with described vacuum pump one end, the valve-closed port of first three-way magnetic valve and the normally opened valve port of the second three-way magnetic valve are equal
It is connected with the vacuum pump other end；The public valve port of first three-way magnetic valve is connected with waste fluid container, and the described 2nd 3
The public valve port of three-way electromagnetic valve is connected with atmosphere.
Preferred technical solution further includes that waste liquid excludes channel, and it includes third threeway electromagnetism that the waste liquid, which excludes channel,
Valve, external waste liquid barrel, waste liquid quantity sensor, the waste liquid quantity sensor are installed together with waste fluid container, to measure waste liquid appearance
In device waste liquid number, the waste liquid quantity sensor can be liquid level sensor, gravity sensor, photoelectric sensor, Huo Zheqi
He is any to can reflect the how many sensor of waste liquid；The third three-way magnetic valve is connected on the waste fluid container and two described
Between two three-way electromagnetic valves, and the public valve port of the third three-way magnetic valve is connected with waste fluid container, the third threeway electromagnetism
The normally opened valve port of valve is connected with one end of 2/2-way solenoid valve, the valve-closed port of the third three-way magnetic valve and one it is external
Waste liquid barrel is connected.
Preferred technical solution further includes flushing channel, and the flushing channel includes the 4th three-way magnetic valve, and described the
Four three-way magnetic valves are connected between the first sheath fluid circulating pump and the sheath fluid buffer storage, wherein the described 4th 3 is powered
The normally opened valve port of magnet valve is connect with the sheath fluid entrance of the sheath fluid buffer storage, the valve-closed port of the 4th three-way magnetic valve with
The sheath fluid entrance of the flow chamber connects, the public valve port of the 4th three-way magnetic valve and the sheath of the first sheath fluid circulating pump
Liquid outlet connection.
Preferred technical solution further includes that automatic sheath fluid continues liquid channel, and it includes the 5th that the automatic sheath fluid, which continues liquid channel,
Three-way magnetic valve, external sheath fluid bucket and sheath fluid quantity sensor, the sheath fluid quantity sensor can be liquid level sensor, gravity sensitive
Device, photoelectric sensor or other any can reflect the how many sensor of sheath fluid；The sheath fluid quantity sensor and sheath liquid container
It is installed together, the number of sheath liquid container mesotheca liquid measured；5th three-way magnetic valve is connected on second sheath fluid and follows
Between ring pump and the sheath fluid buffer storage, wherein the normally opened valve port of the 5th three-way magnetic valve and the sheath fluid buffer storage
Connection, the valve-closed port of the 5th three-way magnetic valve are connect with the external sheath fluid bucket, the public affairs of the 5th three-way magnetic valve
Valve port is connect with the second sheath fluid circulating pump altogether.
A kind of control method of flow cytometer liquid fluid system of the utility model, comprising the following steps:
One, sample introduction focusing, under the action of negative-pressure power system, sample liquid flows into flow chamber, sheath fluid through sample liquid channel
Sheath fluid in buffer storage also flows into flow chamber；Wherein, when the liquid level in sheath fluid buffer storage is higher than and the second sheath fluid circulating pump
When the sheath fluid outlet of connection, the second sheath fluid circulating pump sucks back the sheath fluid for being higher by sheath fluid outlet in sheath liquid container；When sheath fluid is slow
When liquid level in cryopreservation device is lower than the sheath fluid outlet being connected to the second sheath fluid circulating pump, the second sheath fluid circulating pump draws air into sheath
In liquid container, i.e., ensure to flow into the sheath in the sheath fluid exit of flow chamber always in sample introduction focusing from sheath fluid buffer storage
Liquid potential energy is kept constant；
Two, liquid stream monitors process, and sheath flow meters and sheath fluid adjuster form a real-time feedback control circuit；Negative pressure passes
Sensor and vacuum pump group are at a real-time feedback control circuit；Sample liquid flowmeter, sheath flow meters, sheath fluid adjuster, negative pressure source,
And signal processing system constitutes a real-time feedback control circuit；When normal work, negative pressure source and sheath fluid adjuster are by pre-
Fixed parameter work；When sheath fluid channel is interfered by extraneous factor, sheath fluid adjuster carries out the extraneous variation of automatic compensation, really
The sheath flow quantity flowed into flow chamber is protected to keep stablizing；When sample flow sensor detects that sample flow changes, control
The height of host adjust automatically vacuum pump and sheath fluid adjuster (coarse adjustment) processed and sheath fluid buffer storage relative to sample liquid entrance
(accurate adjustment), so that sheath flow quantity is identical as the ratio of sample liquid flow and normal value；Output negative pressure alarm is preset in control host
Range, when exporting negative pressure in the output negative pressure alarm range that system is arranged, it is stifled that control host will start system pipeline part
Plug alarm, and prompt to carry out unimpeded flushing；When exporting negative pressure and exceeding the output negative pressure preset range upper limit, control host is provided
System jams are seriously alarmed, it is necessary to be stopped sample introduction, and be started cleaning process；
Three, shift process tables look-up to obtain the numerical value of sample liquid flow, vacuum pump is adjusted, so that sample liquid stream after replacing gear
Amount reaches the numerical value；Vacuum pump is also in always and stablizes in feedback output simultaneously；Sheath fluid pressure regulator is adjusted, so that sheath liquid stream
The ratio of amount and sample liquid flow in the normal range, completes " coarse adjustment " process.Continue the height of adjusting sheath fluid buffer storage, so that
The ratio of sheath flow quantity and sample liquid flow is identical as normal value, completes gear shift " accurate adjustment " process；
Four, cleaning process, the cleaning process are as follows: the normally opened valve port of the 4th three-way magnetic valve is closed, valve-closed port is opened,
Increase the power of the first sheath fluid circulating pump and the second sheath fluid circulating pump, sheath fluid is directly entered flowing by the 4th three-way magnetic valve
Room, a part of sheath fluid flow into waste fluid container along waste fluid channel, and a part of sheath fluid is discharged in centrifuge tube by sample liquid channel is inversely outer；
Five, waste liquid automatic-discharging process is provided with the corresponding threshold value of waste liquid quantity sensor in the control host of the system, when
When the numerical value of waste liquid amount sensor feedback is greater than the max-thresholds of setting, the normally opened valve port closing of the first, second three-way magnetic valve,
Valve-closed port is opened；Vacuum pump is switched to the mode of inflating by extraction pattern；The normally opened valve port of third three-way magnetic valve closes, is normally closed
Valve port is opened；Under the action of vacuum pump, the waste liquid in waste fluid container is pushed in external waste liquid barrel, until waste liquid amount senses
The numerical value of device feedback is less than the minimum threshold of setting, and the opposite operation of process, completes the automatic-discharging of waste liquid before executing.
Six, sheath fluid automatic liquid replenishing process is provided with the corresponding threshold value of sheath fluid quantity sensor in the control host of the system, when
When the numerical value of sheath fluid quantity sensor feedback is less than the minimum pressure threshold of setting, control host opens the second sheath fluid circulating pump, the
The normally opened valve port of five three-way magnetic valves is closed, valve-closed port is opened, and the sheath fluid in external sheath fluid bucket is in the second sheath fluid circulating pump
It is pumped down under effect in sheath liquid container, until when the numerical value of sheath fluid quantity sensor feedback is greater than the max-thresholds of setting, before execution
Sheath fluid automatic liquid replenishing process is completed in the opposite operation of process.
The power source of the utility model is only a vacuum pump negative pressure source.On channel from negative pressure source to waste fluid container successively
There are two the first three-way magnetic valves in parallel and the second three-way magnetic valve and a B/P EGR Back Pressure Transducer EGR.B/P EGR Back Pressure Transducer EGR detection
Negative pressure and vacuum pump group in waste fluid container realize stable negative pressure output at circuit.The mesh of two three-way magnetic valves in parallel
Be to realize and the waste liquid in waste fluid container is discharged into external waste liquid barrel.In fact, if you do not need to if this function,
The first three-way magnetic valve is only needed, at this time the purpose of the three-way magnetic valve are as follows: after instrument completes work, discharge in waste fluid container
Pressure.
, in negative pressure source channels, part relevant to waste liquid is referred to as waste fluid channel, including waste fluid container, one for we
A waste liquid quantity sensor, a three-way magnetic valve, an external large capacity waste liquid barrel and a 2/2-way solenoid valve.
Waste fluid container public valve port phase with B/P EGR Back Pressure Transducer EGR, the public valve port of the first three-way magnetic valve and third three-way magnetic valve simultaneously
Even.When normal work, from flow chamber flow out waste liquid successively pass through 2/2-way solenoid valve, third three-way magnetic valve enter it is useless
In liquid container.When the numerical value of waste liquid quantity sensor reaches max-thresholds, the first threeway, the second threeway and third three-way magnetic valve
Normally opened valve port close, valve-closed port is opened, vacuum pump becomes the mode of inflating from extraction pattern when logical, thus by waste fluid container
Waste liquid be pressed into external large capacity waste liquid barrel.When the numerical value of waste liquid quantity sensor is less than minimum threshold, execute opposite
Discharge waste liquid process is completed in operation.
Sample liquid channel specifically includes that sample introduction needle, sample liquid flowmeter.Under the action of negative pressure, sample liquid is from centrifuge tube through sample introduction needle
Into flow chamber, flowmeter is mounted in sample introduction needle, to monitor the flow of sample liquid.
Sheath fluid passage component includes: check (non-return) valve, sheath liquid filter, sheath fluid adjuster, sheath flow meters, position potential energy stabilization
Device, the 4th three-way magnetic valve, the 5th three-way magnetic valve, sheath fluid quantity sensor and external large capacity sheath fluid bucket.In negative pressure source
Under effect, sheath fluid successively passes through flowmeter, sheath fluid adjuster, sheath liquid filter and stops from the outlet of position potential energy stabilising arrangement
Inverse valve enters flow chamber.4th three-way magnetic valve is used for detergent line, the 5th three-way magnetic valve, sheath fluid quantity sensor and extraneous sheath
Liquid bucket is used to supplement sheath fluid, detailed in Example to liquid fluid system.Sheath flow meters are used to measure sheath flow quantity, according to its numerical value reality
When adjust negative pressure source parameter, sheath fluid regulator parameter, the parameters such as position potential energy stabilising arrangement height, the stabilization to realize sample liquid is poly-
Coke is detailed in liquid stream control embodiment.Sheath liquid filter is used to filter the little particles such as impurity, the bubble in sheath fluid；Check (non-return) valve is used to
Prevent the liquid in flow chamber from flowing back to sheath fluid channel.
Sheath fluid adjuster is used to directly control sheath flow quantity or controls pressure drop to indirectly control the flow of sheath fluid, according to
The relationship of pressure drop and speed:
Δ P=32 μ lv/d2+ξv2/2g①
Wherein, Δ P is pressure drop, and v is speed, and ξ is the flow channel shape factor, and μ is hydrodynamic viscosity, and l is flow channel length,
D is flow diameter, and g is acceleration of gravity.
Therefore the pressure drop adjusted on sheath fluid runner is just adjustable the speed of sheath fluid, to adjust the flow of sheath fluid.This reality
It can be electronics Valve for compressing tubular liner, electromagnetic throttle valve, flow proportion valve, pressure damper etc. with novel middle sheath fluid adjuster.To sheath fluid
For flow, sheath fluid adjuster not will increase " under negative pressure source effect, the attainable maximum stream flow of sheath fluid ", therefore, we
It can be referred to as " passive-type " sheath fluid adjuster.Benefit using this device is 1) will not to introduce sheath fluid pulsation, 2) it will not make
Dissolve in sheath fluid at gas, 3) for opposite " active " device, volume small power consumption, accessory kit are simple.
Mode, the available sample liquid focal width formula of application quality conservation theorem are flowed into for the sheath fluid of the utility model
Are as follows:
Wherein, d is the focal width of sample liquid；ρ is the indoor fluid density of flow focusing, and D is the waterpower of flow focusing room
Diameter, ρ 1 are sample liquid density；ρ 2 is sheath fluid density；Q1 is sample liquid flow, and Q2 is sheath flow quantity.
It follows that focal width is directly proportional to the flow-rate ratio of sample liquid and sheath fluid, adjusts stream after flowing chamber size determines
Amount is just adjustable focus state.Therefore, the utility model can be realized pair using single negative pressure power source and sheath fluid adjuster
The accurate control of focused flow forms stable unicellular stream.
It is described above it is found that control focus be mainly shown as control sample liquid and sheath fluid flow-rate ratio.In the utility model, sample
Flow meters, sheath flow meters, sheath fluid adjuster, negative pressure source and signal processing system constitute a real-time feedback control
Circuit.When normal work, negative pressure source and sheath fluid adjuster work by scheduled parameter.When external environment changes, such as sample introduction needle
Partial Blocking, sample liquid flow Q1 is reduced at this time.Known by formula 2, sample liquid focal width d becomes smaller at this time, needs to change Q2.It is logical first
It crosses look-up table and obtains the target value of Q2, sheath fluid adjuster is then adjusted, while monitoring sheath flow meters, so that Q2 is decreased to target
Value, to guarantee that focal width d is constant.At this point, the bulk velocity of liquid stream is slack-off, the individual cells that photodetector detects dissipate
Penetrate the frequency shift of signal.It adjusts negative pressure source and exports bigger negative pressure, can compensate for the variation of speed, so that the frequency of scattered signal changes
Fade to original value.If the output increment of negative pressure source is in default range at this time, system will give Partial Blocking warning, and mention
Show and carries out unimpeded flushing.If the output increment of negative pressure source has been more than preset range, illustrate to block more serious, needs to rush immediately
It washes, system will provide alarm, and stop sample introduction work.
The circuit may also used to realize the control to " sample liquid flow gear ".Top grade is adjusted to when needing to realize from low grade
When, increase the output of negative pressure.At this point, sample liquid flow Q1 increases, focal width d increases.According to formula 2, sheath fluid adjuster is adjusted,
So that Q2 changes to corresponding target value.To realize the adjusting of sample liquid gear, and do not change focus state.
Above method may be implemented sample liquid focusing stability contorting, but with the proviso that sheath fluid and sample liquid can stablize it is defeated
Enter.Since sheath flow quantity is far longer than sample liquid flow, so its stability plays absolute leading role.
In the actual process, with the consumption of sheath fluid, the position potential energy of sheath fluid is constantly being reduced.This system is exerted using uncle
Sharp theorem, flow section are chosen at the horizontal plane and sheath fluid tube inlet of sheath fluid respectively, then have:
P0/ρg+h+α1v0 2/ 2g=P1/ρg+α2v2 2/2g+ΔP③
Wherein, P0 is atmospheric pressure, and h is the height of sheath liquid level, and v0 is the decrease speed of liquid in sheath fluid bottle；P1 is sheath fluid
The negative pressure of inlet；V2 is the speed in sheath liquid pipe；Δ P is the pressure loss caused by viscose movements.α is correction factor.
According to flow conservation, 3. formula always be can be written as:
P0/ ρ g+h=P1/ρg+βv2 2/2g+ΔP④
It can be seen that when that is, sheath fluid is consumed, the speed in sheath liquid pipe is changing always, so as to cause sheath when h reduces
Flow quantity is changing.According to formula 2, this focusing that will lead to flow chamber generates variation.Make speed constant, only persistently changes
It is changed into the negative pressure on sheath fluid.As previously described, this compensation precision is not high, or even gradient can only be selected to compensate.
The core concept of the position potential energy stabilising arrangement of the utility model are as follows: even if sheath fluid is consumed, also can guarantee sheath fluid
The height h of sheath fluid is constant in container.A kind of resulting device includes a sheath liquid container, for containing sheath fluid；One sheath
Liquid buffer storage, the buffer storage are a single cavity, have an inlet, two liquid outlets；First sheath fluid circulation
Pump, which is connected with inlet, sheath fluid is withdrawn and fed into sheath fluid buffer storage from sheath liquid container；Second sheath fluid follows
Ring pump, which is connected with one of liquid outlet of sheath fluid buffer storage, to extract simultaneously sheath fluid out from sheath fluid buffer storage
It is injected back into sheath liquid container.Another liquid outlet of sheath fluid buffer storage is connected to flow chamber, and the position of the liquid outlet is lower than
The position for the liquid outlet being connected with the second sheath fluid circulating pump.
Its course of work are as follows: sheath fluid is injected into sheath fluid buffer storage by the first sheath fluid circulating pump, when water level is higher than and second
When the liquid outlet that sheath fluid circulating pump is connected, the sheath fluid being higher by is sucked back into sheath liquid container by the second sheath fluid circulating pump, to guarantee
Liquid level in sheath fluid buffer storage is always the difference in height of two liquid outlets.
Sheath fluid buffer storage is that the possible problem of single cavity is: system stability is easy by the first sheath fluid circulating pump
Injection act influence.Preferably, which can be using the container with multiple chambers, such as linker.Such as
When with two chambers, buffer storage includes a sheath fluid injection region, and sheath fluid injection region is equipped with an inlet；It further include one
Sheath fluid stable region is equipped with a resorption mouth and a sheath fluid outlet.Injection region is connected to stable region by an overcurrent mouth.For
Guarantee that the sheath fluid of injection region flows into stable region in time, it is preferred that the area of overcurrent mouth is greater than the area of inlet.Sheath fluid is stablized
Resorption mouth and sheath fluid outlet in area have difference in height, the as h in above formula.
First sheath fluid circulating pump continues to be injected into sheath fluid injection region from sheath liquid container by sheath fluid, when water level exceeds overcurrent mouth
When, the sheath fluid being higher by will flow into sheath fluid stable region.And when the water level in sheath fluid stable region exceeds sheath fluid resorption mouth, it is higher by
Sheath fluid will be drawn back into sheath liquid container by the second sheath fluid circulating pump.
Due to the sheath fluid injected from inlet, a part enters in flow chamber from sheath fluid outlet, it is therefore desirable to from sheath fluid
The amount of resorption mouth resorption be less than injection rate, simultaneously because sheath fluid rate of discharge is less than sheath fluid inlet flow, therefore will not go out
Existing sheath fluid is accumulated in sheath fluid stable region or the scene of reduction, ensure that sheath fluid water level and sheath fluid resorption oral pore flush, Ye Jibao
The position potential energy perseverance for having demonstrate,proved sheath fluid exit is h.
The hole location that the utility model need to only meet sheath fluid resorption mouth exceeds the hole location of sheath fluid outlet, and to the height in other holes
No requirement (NR).
It is also practical to entire liquid fluid system although formula 4 is to be drawn a conclusion to sheath fluid using bernoulli theorem.
Only h should be sensu lato height including sample liquid entry position.Specifically, h is that sheath fluid buffer storage (flows into flowing
Room) liquid outlet, the difference in height with sample injection port.
According to formula 4, changing this highly will affect the flow of sheath fluid, and then influence the focusing of sample liquid.In general, this is high
Degree just secures after equipment sizing.But we can further using this height, such as by sheath fluid buffer storage it is vertical on
Lower movement.In fact, the precision of current guide rail or screw rod completely can be high to adjust this with millimeter, even micron dimension
Degree.Influence of the Level Change of this magnitude to focusing, compared with the 2nd point in change negative pressure source and sheath fluid regulator parameter and come more
It is accurate.Therefore this method is called accurate adjustment by we, and the method for adjusting negative pressure source and sheath fluid adjuster call coarse adjustment (though
So it is also very accurate).
In summary the utility model has the beneficial effects that:
1, sheath fluid flow control is accurate.Sheath fluid channel is provided with position potential energy stabilising arrangement, the position potential energy stabilising arrangement
It is set on the sheath fluid buffer storage for including there are two liquid outlet, is connected wherein the liquid outlet being connected with sheath liquid container is higher than with flow chamber
Liquid outlet, and then guarantee flowed to from sheath fluid buffer storage flow chamber sheath fluid position potential energy it is constant, and the device not against
Sensor has very high precision and quick response degree, while cost is also very low.Flow sensor use is provided in sheath fluid channel
To monitor the flow of sheath fluid；And sheath fluid adjuster is used to adjust the flow on sheath fluid channel, cooperation negative pressure device is able to achieve liquid
Pressure-flow control of streaming system.Compared to existing sheath fluid control method, the utility model does not need to adjust sheath fluid tune in real time
Device is saved, is realized simply, fast precision height is responded, has prevented sheath fluid pulsation phenomenon.
2, the monitoring of sample liquid focus state and accurate control.Sample liquid flow sensor, sheath fluid flow sensor, sheath fluid are adjusted
Device, negative-pressure power system and signal processing system form a control loop, and the monitoring and control to sample liquid state may be implemented
System, the gear for changing sample liquid will not influence the focus state of sample liquid.Meanwhile sheath fluid buffer storage being cooperated to move up and down, it can be with
Realize the accurate control of focus state.
3, sample introduction is carried out using single negative-pressure power system and worked into sheath fluid, negative-pressure power system and B/P EGR Back Pressure Transducer EGR composition
One control loop realizes the precise and stable output of negative pressure.Sheath fluid end uses " passive-type " sheath fluid adjuster, so do not need close
Envelope, air will not dissolve in sheath fluid, greatly reduce a possibility that bubble occurs.And in comparison, " passive-type " sheath fluid is adjusted
Device and its related accessories are small in size, low in energy consumption, almost noiseless.Meanwhile the characteristic of blow-by reduces the use cost of user
And consumables cost.
4, utility model provides the control and method for being concisely and efficiently cleaning, unimpeded.
5, the utility model may be implemented the automatic-discharging of waste liquid and the automatic continuous of sheath fluid plus.External discharging of waste liquid channel
It may be located remotely from equipment and place elsewhere, and then the waste fluid container of large capacity can be used；Equally, external sheath liquid container can also be with
Using large-rolume container.Therefore, entire liquid fluid system can be packaged in instrument internal, only there are sheath fluid entrance, sample liquid entrance and
Waste liquid outlet.The sheath fluid bucket and waste liquid barrel of the external large capacity of user can be achieved with prolonged steady operation, to avoid
User, which frequently opens sheath fluid bottle addition sheath fluid, to be influenced system stability bring and the problems such as cross contamination.Entirely
System automation degree is high.
Detailed description of the invention
Fig. 1 is the sheath fluid buffer storage section view of the single-chamber body of the flow cytometer liquid fluid system of the utility model embodiment 1
Figure.
Fig. 2 be the utility model embodiment 1 flow cytometer liquid fluid system in position potential energy stabilising arrangement schematic diagram with
Work flow diagram.
Fig. 3 is the structural schematic diagram of the flow cytometer liquid fluid system of the utility model embodiment 1.
Fig. 4 is the structural schematic diagram of the flow cytometer liquid fluid system of the utility model embodiment 2.
Fig. 5 is the structural schematic diagram of the flow cytometer liquid fluid system of the utility model embodiment 3.
Fig. 6 is the liquid-flow state monitoring and control flow original of the flow cytometer liquid fluid system of the utility model embodiment 3
Reason figure.
Fig. 7 is the sample liquid shift control flow schematic diagram of the flow cytometer liquid fluid system of the utility model embodiment 3.
Specific embodiment
The embodiments of the present invention are described in detail with reference to the accompanying drawing.
Embodiment 1
Fig. 1 is the sheath fluid buffer storage cross-sectional view of single-chamber body.Sheath fluid buffer storage 33 is at least one entrance and at least
Two outlets, wherein entrance 333 is connected to the first sheath fluid circulating pump, and outlet 331 is connected to flow chamber, outlet 332 and the second sheath
The connection of liquid circulating pump.Height of the height of outlet 331 lower than outlet 332.Sheath fluid is injected into caching dress by the first sheath fluid circulating pump
In setting, when liquid level is higher than outlet 332, the sheath fluid being higher by is extracted out by the second sheath fluid circulating pump.The height of outlet 332 and outlet 331
Degree difference just determines the position potential energy of sheath fluid, and keeps constant.Sheath fluid buffer storage passes through an air filter and atmosphere
Connection.
Fig. 2 is position potential energy stabilising arrangement schematic diagram and work flow diagram.The position potential energy stabilising arrangement includes containing
The sheath liquid container 361 of sheath fluid, the first sheath fluid circulating pump 35, sheath fluid buffer storage 33, the second sheath fluid circulating pump 37.Fig. 2 is to sheath
Liquid buffer storage has carried out section view, and there is the sheath fluid buffer storage at least one sheath fluid entrance 333, at least two sheath fluids to export
331,332；The entrance of the first sheath fluid circulating pump 35 is connect with the sheath liquid container 361, the first sheath fluid circulating pump 35
Outlet connect with the sheath fluid entrance 333 of the sheath fluid buffer storage 33；At least one sheath fluid of the sheath fluid buffer storage 33
Outlet 331 is connected to flow chamber, at least one sheath fluid outlet 332 and second sheath fluid of the sheath fluid buffer storage 33 recycle
The entrance connection of pump 37,37 outlet is connected to sheath liquid container 361.The height of 33 middle outlet 332 of sheath fluid buffer storage is higher than it
The height of outlet 331, while the sheath flow quantity for flowing to the sheath fluid buffer storage 33 through the first sheath fluid circulating pump 35 is greater than
Equal to the sheath flow quantity that the sheath fluid buffer storage 33 flows to the flow chamber.Sheath fluid buffer storage 33 at least has there are two chamber
Body, sheath fluid entrance 333 are disposed therein on a cavity 335, and (i.e. structure is arranged on another cavity 336 in outlet 331,332
At having a sheath fluid in a sheath fluid buffer storage with respect to stability region).Cavity 335 and cavity 336 are separated with partition, are passed through
The through-hole 334 being arranged on partition is connected to；The sheath fluid buffer storage 33 is communicated with the atmosphere by an air filter.
Embodiment 2
As shown in figure 3, a kind of flow cytometer liquid fluid system comprising sample liquid channel 1, flow chamber 2,3 and of sheath fluid channel
Negative pressure source channels 4, the sample liquid channel 1, sheath fluid channel 3, negative pressure source channels 4 are connect with the flow chamber 2；The sample liquid is logical
Road 1 includes the feed liquor sample needle 10 and sample liquid flow sensor 11 being set in sequence, and the sample liquid in the feed liquor sample needle 11 passes through sample liquid
It is flowed into after flow sensor 12 in the flow chamber；
The negative pressure source channels 4 are successively including vacuum pump 41, the first three-way magnetic valve 42, waste fluid container 441 and two two
Three-way electromagnetic valve 45；The vacuum pump 41 is connected with the normally opened valve port of the first three-way magnetic valve 42, the first three-way magnetic valve 42 it is normal
Valve closing mouth and atmosphere are connected, and public valve port is connect with waste fluid container 441；The waste fluid container 441 is through the 2/2-way electricity
It is connect after magnet valve 45 with the flow chamber 2, a B/P EGR Back Pressure Transducer EGR 43 is in addition connected on waste fluid container 441.
The sheath fluid channel 3 is provided with position potential energy stabilising arrangement, and the position potential energy stabilising arrangement includes containing sheath fluid
Sheath liquid container 361, the first sheath fluid circulating pump 35, sheath fluid buffer storage 33, the second sheath fluid circulating pump 37.The sheath fluid caching
Device has at least one sheath fluid entrance 333, at least two sheath fluids outlet 331,332, and passes through air filter and atmosphere
Connection；The entrance of the first sheath fluid circulating pump 35 is connect with the sheath liquid container 361, and the first sheath fluid circulating pump 35 goes out
Mouth is connected to the sheath fluid entrance 333 of the sheath fluid buffer storage 33；At least one sheath fluid of the sheath fluid buffer storage 33 exports
331 are connected to flow chamber, at least one sheath fluid outlet 332 and the second sheath fluid circulating pump 37 of the sheath fluid buffer storage 33
Entrance connection, 37 outlet is connected to sheath liquid container 361.The height of 33 middle outlet 332 of sheath fluid buffer storage is higher than its outlet
331 height, while the sheath flow quantity for flowing to the sheath fluid buffer storage 33 through the first sheath fluid circulating pump 35 is more than or equal to
The sheath fluid buffer storage 33 flows to the sheath flow quantity of the flow chamber.The sheath fluid buffer storage 33 and the flow chamber 2 it
Between be also successively arranged flow sensor 32, sheath fluid adjuster 31, sheath liquid filter 301, check (non-return) valve 300.
Embodiment 3
As shown in figure 4, a kind of flow cytometer liquid fluid system comprising sample liquid channel 1, flow chamber 2,3 and of sheath fluid channel
Negative pressure source channels 4, the sample liquid channel 1, sheath fluid channel 3, negative pressure source channels 4 are connect with the flow chamber 2；The sample liquid is logical
Road 1 includes the feed liquor sample needle 10 and sample liquid flow sensor 11 being set in sequence, and the sample liquid in the feed liquor sample needle 11 passes through sample liquid
It is flowed into after flow sensor 12 in the flow chamber.
The negative pressure source channels 4 are successively including vacuum pump 41, the first three-way magnetic valve 42, waste fluid container 441 and two two
Three-way electromagnetic valve 45；The vacuum pump 41 is connected with the normally opened valve port of the first three-way magnetic valve 42, the first three-way magnetic valve 42 it is normal
Valve closing mouth and atmosphere are connected, and public valve port is connect with waste fluid container 441；The waste fluid container 441 is through the 2/2-way electricity
It is connect after magnet valve 45 with the flow chamber 2, a B/P EGR Back Pressure Transducer EGR 43 is in addition connected on waste fluid container 441.
The sheath fluid channel 3 is provided with position potential energy stabilising arrangement, and the position potential energy stabilising arrangement includes containing sheath fluid
Sheath liquid container 361, the first sheath fluid circulating pump 35, three-way magnetic valve 34, sheath fluid buffer storage 33, the second sheath fluid circulating pump
37.The sheath fluid buffer storage has at least one sheath fluid entrance 333, at least two sheath fluids outlet 331,332, and passes through sky
Air filter is communicated with the atmosphere；The entrance of the first sheath fluid circulating pump 35 is connect with the sheath liquid container 361, first sheath
The outlet of liquid circulating pump 35 is connect with the public valve port of three-way magnetic valve 34, the valve-closed port of three-way magnetic valve 34 directly with flowing
Room 2 connects, and normally opened valve port is connected to the sheath fluid entrance 333 of the sheath fluid buffer storage 33；The sheath fluid buffer storage 33
The outlet of at least one sheath fluid 331 is connected to flow chamber, at least one sheath fluid of the sheath fluid buffer storage 33 export 332 with it is described
The entrance of second sheath fluid circulating pump 37 is connected to, and 37 outlet is connected to sheath liquid container 361.33 middle outlet 332 of sheath fluid buffer storage
Height be higher than the height of its outlet 331, while flowing to the sheath fluid buffer storage 33 through the first sheath fluid circulating pump 35
Sheath flow quantity is more than or equal to the sheath flow quantity that the sheath fluid buffer storage 33 flows to the flow chamber.The sheath fluid buffer storage 33
Flow sensor 32, sheath fluid adjuster 31, sheath liquid filter 301, check (non-return) valve 300 are also successively arranged between the flow chamber 2.
Embodiment 4
As shown in figure 5, a kind of flow cytometer liquid fluid system comprising sample liquid channel 1, sheath fluid channel 3, negative pressure source channels
4 and flow chamber 2, the sample liquid channel 1, sheath fluid channel 3, negative pressure source channels 4 connect with the flow chamber 2；The sample liquid is logical
Road 1 includes the feed liquor sample needle 11 and sample liquid flow sensor 12 being set in sequence, and the sample liquid in the feed liquor sample needle 11 passes through sample liquid
It is flowed into after flow sensor 12 in the flow chamber；
The negative pressure source channels 4 are successively including negative pressure source device, waste fluid container 441, third three-way magnetic valve 47 and two
Two three-way electromagnetic valves 45；The negative pressure source device includes vacuum pump 41, the first three-way magnetic valve 42 and the second three-way magnetic valve 46；Institute
It states the first three-way magnetic valve and the second three-way magnetic valve is in parallel；The normally opened valve port of first three-way magnetic valve 42 and the second threeway
The valve-closed port of solenoid valve 46 is connected to one end of vacuum air pump 41 simultaneously；The valve-closed port of the first threeway electromagnetism 42 and
The normally opened valve port of two three-way magnetic valves 46 is connected to the other end of vacuum air pump 41 simultaneously；The public valve port and waste liquid of solenoid valve 42
Container 441 is connected, and the public valve port and atmosphere of solenoid valve 46 are connected.B/P EGR Back Pressure Transducer EGR 43 is further connected on waste fluid container 441.Waste liquid
Quantity sensor 442 is also mounted on waste fluid container 441, for detecting the amount of waste liquid in 441.Waste liquid quantity sensor 442 can be
Liquid level sensor, gravity sensor, photoelectric sensor or other any can reflect the how many sensor of waste liquid.It is described useless
Liquid container 441 is also connected with the public valve port of third three-way magnetic valve 47, the valve-closed port of third three-way magnetic valve 47 with it is external
Large capacity waste liquid barrel 48 be connected, 47 normally opened valve port is connected by 2/2-way solenoid valve 45 and flow chamber 2.
The sheath fluid channel 3 is provided with position potential energy stabilising arrangement, and the position potential energy stabilising arrangement includes containing sheath fluid
Sheath liquid container 361, the first sheath fluid circulating pump 35, the 4th three-way magnetic valve 34, sheath fluid buffer storage 33, the 5th threeway electromagnetism
Valve 38, the second sheath fluid circulating pump 37.There is the sheath fluid buffer storage at least one sheath fluid entrance 333, at least two sheath fluids to go out
Mouth 331,332, and be communicated with the atmosphere by air filter；The entrance and the sheath fluid of the first sheath fluid circulating pump 35 hold
Device 361 connects, and the outlet of the first sheath fluid circulating pump 35 is connect with the public valve port of three-way magnetic valve 34, three-way magnetic valve 34
Valve-closed port directly connect with flow chamber 2, normally opened valve port is connected to the sheath fluid entrance 333 of the sheath fluid buffer storage 33；
At least one sheath fluid outlet 331 of the sheath fluid buffer storage 33 is connected to flow chamber, and the sheath fluid buffer storage 33 is at least
One sheath fluid outlet 332 is connected with the normally opened valve port of the three-way magnetic valve 38, the valve-closed port of the three-way magnetic valve 38 and
External large capacity sheath fluid bucket is connected, and 38 public valve port is connected to the entrance of the second sheath fluid circulating pump 37,37 outlet and sheath
Liquid container 361 is connected to.The height of 33 middle outlet 332 of sheath fluid buffer storage is higher than the height of its outlet 331, while through described first
The sheath flow quantity that sheath fluid circulating pump 35 flows to the sheath fluid buffer storage 33 is more than or equal to the sheath fluid buffer storage 33 and flows to institute
State the sheath flow quantity of flow chamber.Also be successively arranged between the sheath fluid buffer storage 33 and the flow chamber 2 flow sensor 32,
Sheath fluid adjuster 31, sheath liquid filter 301, check (non-return) valve 300.Sheath fluid quantity sensor 362 is also equipped on sheath liquid container 361,
For detecting the amount of sheath fluid in 361, the sheath fluid sensor 362 can be liquid level sensor, gravity sensor, photoelectric sensing
Device or other any can reflect the how many sensor of waste liquid
As shown in Figures 5 to 7, the working principle of the present embodiment is as follows:
One, sample introduction focusing
Vacuum pump 41 is in extraction pattern, forms negative pressure in waste fluid container 441 by the first triple valve 42, the negative pressure is logical
Cross third triple valve 47, two-way valve 45 acts on flow chamber 2 so that sample liquid through sample liquid channel flow into flow chamber, sheath fluid
Sheath fluid in buffer storage also flows into flow chamber, and forms stable focusing.Wherein, when the liquid level in sheath fluid buffer storage 33 is high
When the sheath fluid for being connected to sheath liquid container 361 exports 332, the second sheath fluid circulating pump 37 is by the sheath being higher by sheath fluid buffer storage
Liquid is retracted in sheath liquid container；When in sheath fluid buffer storage 33 liquid level is lower than the sheath fluid outlet 332 for being connected to sheath liquid container 361
When, the second sheath fluid circulating pump is drawn air into sheath liquid container 361, i.e., ensures to cache from sheath fluid always in sample introduction focusing
The sheath fluid potential energy flowed at the sheath fluid outlet 331 of flow chamber in device is kept constant.
Two, monitoring process is focused
In actual motion, focusing is simultaneously unstable constant.The generation of external disturbance, bubble and sample introduction needle are stifled
Plug, which can all cause to focus, to fail.Therefore, it is necessary to be monitored to focusing.
In the utility model, sheath flow meters and sheath fluid adjuster form a real-time feedback control circuit；Negative pressure sensing
Device and vacuum pump group are at a real-time feedback control circuit；Sample liquid flowmeter, sheath flow meters, sheath fluid adjuster, negative pressure source, with
And signal processing system constitutes a real-time feedback control circuit.The B/P EGR Back Pressure Transducer EGR 43 being mounted on waste fluid container is supervised in real time
The vacuum magnitude in waste fluid container is controlled, by feeding back and adjusting vacuum pump, so that the negative pressure in waste fluid container is kept constant.Normally
When work, negative pressure source and sheath fluid adjuster work by scheduled parameter.When sheath fluid channel is interfered by extraneous factor, sheath fluid tune
It saves device and carries out the extraneous variation of automatic compensation, it is ensured that the sheath flow quantity flowed into flow chamber keeps stablizing.As shown in fig. 6, working as sample
When this flow sensor detects that sample flow changes, sheath fluid adjuster is adjusted, while monitoring sheath flow meters, so that
The value of Q2/Q1 is in normal envelope, to guarantee focal width in error range.At this point, the bulk velocity of liquid stream will
Change, the frequency for the individual cells scattered signal that photodetector detects can also change.Adjust negative pressure source compensation speed
Variation so that the frequency shift of scattered signal is to original value.If the output increment of negative pressure source is in default range at this time,
System will continue the height of adjusting sheath fluid buffer storage, so that the value of Q2/Q1 is identical as normal value, and it is alert to provide Partial Blocking
It accuses, prompts to carry out unimpeded flushing.If the output increment of negative pressure source has been more than preset range, illustrate to block more serious, needs to stand
It rinses, system will provide alarm, and stop sample introduction work.
Three, gearshift procedure
Shift process is considered as focusing the special circumstances of monitoring.As shown in fig. 7, tabling look-up to obtain sample after replacing gear
The numerical value of flow quantity adjusts vacuum pump, so that sample liquid flow reaches numerical value Q1.Vacuum pump is also always in stable feedback simultaneously
In output.Sheath fluid pressure regulator is adjusted, so that the value of Q2/Q1 is in the normal range, completes " coarse adjustment " process.Continue to adjust sheath
The height of liquid buffer storage completes " accurate adjustment " process so that the value of Q2/Q1 is identical as normal value.Replacement gear can't as a result,
Cause the variation of sample liquid focal width, testing result will not be impacted.
Four, cleaning process
After system enters cleaning procedure, the normally opened valve port O of the 4th three-way magnetic valve 34 is closed, and valve-closed port B is opened.Sheath fluid
Under the action of the first sheath fluid circulating pump 35, flow chamber 2 is directly entered by 34 B mouth.A part of sheath fluid passes through 2/2-way
Solenoid valve 45 enters in waste fluid container 441, and another part sheath fluid is recoiled back by sample introduction needle 10 in centrifuge tube, to complete to manage
Road cleaning.When sample introduction needle blocking, it can choose and close two three-way electromagnetic valves 45, so that flushing ability acts in sample introduction needle completely.
After the completion of cleaning, the valve-closed port B of the second three-way magnetic valve is opened, the pressure in closed waste fluid container is discharged.
Five, sheath fluid automatic liquid replenishing process
Sheath fluid quantity sensor 362 and sheath liquid container 361 are installed together, for detecting the amount of sheath fluid in 361.When 362
Other component when numerical value reaches the minimum threshold of setting, except component needed for host will close sheath fluid automatic liquid replenishing.At this point, the
The normally opened valve port O of five three-way magnetic valves 38 is closed, and valve-closed port B is opened.Sheath fluid in external large capacity sheath fluid bucket 39 is by second
Sheath fluid circulation is pumped into sheath liquid container 361.When the numerical value of sheath fluid quantity sensor 362 reaches the max-thresholds of setting, table
Bright sheath fluid has continued full.At this point, closing the valve-closed port B of the 5th three-way magnetic valve 38, normally opened valve port O is opened, is completed automatic continuous
Liquid process.
Six, waste liquid automatic-discharging process
Waste liquid quantity sensor 442 and waste fluid container 4411 are installed together, for detecting the amount of waste liquid in 441.When 442
Other component when numerical value reaches the max-thresholds of setting, except component needed for host will close waste liquid automatic-discharging process.This
When, the normally opened valve port O of third three-way magnetic valve 47 is closed, and valve-closed port B is opened, and waste fluid container 441 and external large capacity are useless
Liquid bucket 48 is connected to.The normally opened valve port of first, second three-way magnetic valve (42,46) is closed, valve-closed port is opened；Vacuum pump 41 is by taking out
Gas pattern switching is to the mode of inflating；Under the action of vacuum pump, the waste liquid in waste fluid container 441 is pushed to external waste liquid barrel 48
In.When the numerical value that waste liquid quantity sensor 442 is fed back is less than the minimum threshold of setting, show that waste liquid is drained.At this point, opening the
One, the opening valve port O of second, third three-way magnetic valve closes valve-closed port B, completes the automatic-discharging of waste liquid.
Specific embodiment of the present utility model above described embodiment only expresses, the description thereof is more specific and detailed,
But it should not be understood as limiting the scope of the patent of the utility model.It should be pointed out that for the common of this field
For technical staff, without departing from the concept of the premise utility, various modifications and improvements can be made, these all belong to
In the protection scope of the utility model.
Claims (9)
1. a kind of flow cytometer liquid fluid system comprising sample liquid channel, sheath fluid channel, negative pressure source channels and flow chamber, it is described
Sample liquid channel, sheath fluid channel, negative pressure source channels are connect with the flow chamber；It is characterized in that, the sheath fluid channel is provided with
Position potential energy stabilising arrangement, the position potential energy stabilising arrangement include containing the sheath liquid container of sheath fluid, sheath fluid buffer storage, the
One sheath fluid circulating pump, the second sheath fluid circulating pump；The sheath fluid buffer storage has at least one sheath fluid entrance, at least two sheath fluids
Outlet；The entrance of the first sheath fluid circulating pump and the sheath fluid reservoir, the outlet of the first sheath fluid circulating pump and institute
State the sheath fluid entrance connection of sheath fluid buffer storage；The outlet of at least one sheath fluid and the flow chamber of the sheath fluid buffer storage connect
Logical, at least one sheath fluid outlet of the sheath fluid buffer storage is connected to the entrance of the second sheath fluid circulating pump, and described second
The outlet of sheath fluid circulating pump and the sheath fluid reservoir, and the sheath fluid being connected in sheath fluid buffer storage with the second sheath fluid circulating pump
Outlet is higher than its sheath fluid outlet being connected with flow chamber, while flowing to the sheath fluid buffer storage through the first sheath fluid circulating pump
Sheath flow quantity be more than or equal to the sheath fluid buffer storage and flow to the sheath flow quantity of the flow chamber.
2. a kind of flow cytometer liquid fluid system according to claim 1, which is characterized in that the sheath fluid buffer storage is
Container with single chamber, sheath fluid entrance and sheath fluid outlet are in the same container.
3. a kind of flow cytometer liquid fluid system according to claim 1, which is characterized in that the sheath fluid buffer storage is
Container with multiple chambers, the sheath fluid entrance of the sheath fluid buffer storage are disposed therein on a chamber, sheath fluid caching dress
The sheath fluid outlet set is arranged on other chambers.
4. a kind of flow cytometer liquid fluid system according to claim 1, which is characterized in that the sheath fluid buffer storage phase
The height of sample liquid sample introduction needle bottom can be accurately adjusted, to realize the precise controlling to fluid focus, the sheath fluid
Buffer storage blow-by is communicated with the atmosphere by an air filter, to guarantee that sheath fluid is clean.
5. a kind of flow cytometer liquid fluid system according to claim 1, which is characterized in that the sheath fluid buffer storage with
Flow sensor, sheath fluid adjuster, sheath liquid filter, check (non-return) valve are also successively arranged between the flow chamber.
6. a kind of flow cytometer liquid fluid system according to claim 1, which is characterized in that the negative pressure source channels include
Negative-pressure power system, waste fluid container, B/P EGR Back Pressure Transducer EGR and 2/2-way solenoid valve；The B/P EGR Back Pressure Transducer EGR and waste fluid container phase
Even and negative-pressure power system forms closed loop, to adjust the pressure in waste fluid container；One end of the 2/2-way solenoid valve and stream
Dynamic room is connected, and the other end is connected to the waste fluid container；The negative-pressure power system include vacuum pump, the first three-way magnetic valve,
Second three-way magnetic valve；First three-way magnetic valve and second three-way magnetic valve are in parallel, and the described 1st is powered
The normally opened valve port of magnet valve is connected with described vacuum pump one end with the normally closed port of the second three-way magnetic valve, the first threeway electromagnetism
The valve-closed port of valve is connected with the vacuum pump other end with the normally opened valve port of the second three-way magnetic valve；Described 1st is powered
The public valve port of magnet valve is connected with waste fluid container, and the public valve port of second three-way magnetic valve is connected with atmosphere.
7. a kind of flow cytometer liquid fluid system according to claim 6, which is characterized in that it further includes that waste liquid excludes to lead to
Road, it includes third three-way magnetic valve, external waste liquid barrel, waste liquid quantity sensor, the waste liquid amount sensing that the waste liquid, which excludes channel,
Device is installed together with waste fluid container, with measure waste liquid in waste fluid container number, the waste liquid quantity sensor is level sensing
Device, gravity sensor or photoelectric sensor；The third three-way magnetic valve is connected on the waste fluid container and the 2/2-way
Between solenoid valve, and the public valve port of the third three-way magnetic valve is connected with waste fluid container, the third three-way magnetic valve
Normally opened valve port is connected with one end of 2/2-way solenoid valve, the valve-closed port of the third three-way magnetic valve and an external waste liquid
Bucket is connected.
8. a kind of flow cytometer liquid fluid system according to claim 1, which is characterized in that it further includes flushing channel,
The flushing channel includes the 4th three-way magnetic valve, and the 4th three-way magnetic valve is connected on the first sheath fluid circulating pump and institute
It states between sheath fluid buffer storage, wherein the sheath fluid of the normally opened valve port and the sheath fluid buffer storage of the 4th three-way magnetic valve enters
Mouth connection, the valve-closed port of the 4th three-way magnetic valve are connect with the sheath fluid entrance of the flow chamber, and the described 4th 3 is powered
The sheath fluid of the public valve port of magnet valve and the first sheath fluid circulating pump, which exports, to be connected.
9. a kind of flow cytometer liquid fluid system according to claim 1, which is characterized in that it further includes that automatic sheath fluid is continuous
Liquid channel, it includes the 5th three-way magnetic valve, external sheath fluid bucket and sheath fluid quantity sensor, the sheath that the automatic sheath fluid, which continues liquid channel,
Liquid measure sensor is liquid level sensor, gravity sensor or photoelectric sensor；The sheath fluid quantity sensor and sheath liquid container are installed
Together, the number of sheath liquid container mesotheca liquid measured；5th three-way magnetic valve is connected on the second sheath fluid circulating pump
Between the sheath fluid buffer storage, wherein the normally opened valve port of the 5th three-way magnetic valve and the sheath fluid buffer storage connect
Connect, the valve-closed port of the 5th three-way magnetic valve is connect with the external sheath fluid bucket, the 5th three-way magnetic valve it is public
Valve port is connect with the second sheath fluid circulating pump.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721706400.XU CN208537364U (en) | 2017-12-08 | 2017-12-08 | A kind of flow cytometer liquid fluid system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721706400.XU CN208537364U (en) | 2017-12-08 | 2017-12-08 | A kind of flow cytometer liquid fluid system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107782657A (en) * | 2017-12-08 | 2018-03-09 | 成都索尔恩科技有限公司 | A kind of flow cytometer liquid fluid system and its control method |
| CN112730204A (en) * | 2020-12-29 | 2021-04-30 | 青岛瑞斯凯尔生物科技有限公司 | Stable liquid path system of flow cytometer |
-
2017
- 2017-12-08 CN CN201721706400.XU patent/CN208537364U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107782657A (en) * | 2017-12-08 | 2018-03-09 | 成都索尔恩科技有限公司 | A kind of flow cytometer liquid fluid system and its control method |
| CN107782657B (en) * | 2017-12-08 | 2023-09-26 | 成都索尔恩科技有限公司 | Flow cytometer liquid flow system and control method thereof |
| CN112730204A (en) * | 2020-12-29 | 2021-04-30 | 青岛瑞斯凯尔生物科技有限公司 | Stable liquid path system of flow cytometer |
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