CN111115827B - Method for removing ammonia nitrogen in molecular sieve wastewater by using microalgae - Google Patents

Method for removing ammonia nitrogen in molecular sieve wastewater by using microalgae Download PDF

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CN111115827B
CN111115827B CN201811278185.7A CN201811278185A CN111115827B CN 111115827 B CN111115827 B CN 111115827B CN 201811278185 A CN201811278185 A CN 201811278185A CN 111115827 B CN111115827 B CN 111115827B
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wastewater
culture
microalgae
molecular sieve
ammonia nitrogen
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CN111115827A (en
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倪黎
魏东
余宗苡
梁维军
王聪慧
韦国有
李斌
袁曙辉
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China Petroleum and Chemical Corp
Sinopec Catalyst Co
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Sinopec Catalyst Co
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia

Abstract

The invention relates to the field of wastewater treatment, and particularly discloses a method for removing ammonia nitrogen in molecular sieve wastewater by using microalgae. The method comprises the step of inoculating microalgae into molecular sieve wastewater containing a basic culture medium for culture. The method provided by the invention can effectively remove ammonia nitrogen in the wastewater, and can realize accumulation of microalgae biomass while purifying the wastewater, so that the treatment cost of the wastewater can be effectively reduced, the comprehensive development and utilization of resources are realized, and the method has economic and environmental-friendly double values.

Description

Method for removing ammonia nitrogen in molecular sieve wastewater by using microalgae
Technical Field
The invention relates to the field of wastewater treatment, in particular to a method for removing ammonia nitrogen in molecular sieve wastewater by using microalgae.
Background
In recent years, with the increase of the demand of catalytic cracking catalysts, the yield of the molecular sieves required by the catalytic cracking catalysts is increased, and the discharge amount of wastewater generated in the preparation process of the molecular sieves is increased year by year. Because the national requirements on the environmental protection of chemical production enterprises are increasingly strict, the green, environmental protection and sustainable development are emphasized in the production process. Therefore, the treatment of industrial wastewater needs to consider whether the water quality purification reaches the standard, and also needs to consider the treatment energy consumption, the cost, the treatment efficiency and the added value of byproducts. Resource utilization is the fundamental way of wastewater treatment. Currently, the most common methods for treating NaY wastewater include air stripping and gas stripping. The main driving force of the air stripping method is the difference between the partial pressure of gas-phase ammonia and the equilibrium partial pressure equivalent to the ammonia concentration in the sewage, under the alkaline environment, the ammonia gas is continuously stripped by air so that the ammonium ions in the wastewater are continuously converted into gas phase, and finally the ammonia is removed, but factors such as temperature, pH and gas-liquid ratio can influence the removing effect of the air stripping method; although the gas stripping method has a very good effect of removing harmful sludge components and ammonium nitrogen, can stabilize activated sludge, and is suitable for large-scale treatment of high-concentration ammonia nitrogen sewage, the stripping effect is not ideal, a large amount of alkali liquor is consumed during large-scale treatment, the total price is high, the energy consumption is large, in addition, the daily maintenance work is complicated, scale is easy to form in an industrial-scale ammonia gas stripping tower, and the treatment is relatively troublesome.
The microalgae has the characteristics of rapid propagation, various nutrition modes, simple growth requirements, strong tolerance and the like, and can synthesize self complex cell components by using carbon, nitrogen and phosphorus in wastewater, thereby effectively removing pollutants in the wastewater as nutrient substances required by propagation of algae, and the microalgae is an algae species widely applied in wastewater treatment in recent years. The method for purifying the wastewater by utilizing the nutritional requirements in the microalgae breeding process is an effective way for realizing sustainable development by harvesting high-value algae biomass and purified water, and has wide attention in the industries of municipal sewage, livestock and poultry breeding wastewater, chemical fertilizers and the like.
Therefore, the efficient, energy-saving and environment-friendly treatment method for the NaY type molecular sieve high ammonia nitrogen wastewater is established, and the method is necessary for realizing the cyclic and sustainable utilization of water resources and the clean production.
Disclosure of Invention
The invention aims to overcome the defects in the prior art, and provides a method for removing ammonia nitrogen in molecular sieve wastewater by using microalgae, which can effectively remove ammonia nitrogen in wastewater, realizes accumulation of microalgae biomass while purifying the quality of wastewater, can effectively reduce the treatment cost of wastewater, realizes comprehensive development and utilization of resources, has economic and environmental-friendly double values, is suitable for purification treatment of wastewater quality in large and medium chemical plants, and is particularly popularized and applied in large-scale enterprises with high-yield and high-ammonia nitrogen wastewater.
In order to achieve the purpose, the invention provides a method for removing ammonia nitrogen in molecular sieve wastewater by using microalgae.
Preferably, the salinity of the molecular sieve wastewater is 10-40 per mill, NH4 +1000-5000mg/L of PO4 3-0-300mg/L of Mn2+、Ca2+、K+、Cu2+、Zn2+、Mg2+、Fe2+Each of which is 0-200 mg/L.
Preferably, the molecular sieve wastewater is supplemented with a carbon source.
Preferably, the concentration of the carbon source additionally added is 0 to 50g/L, more preferably 1 to 20 g/L.
Preferably, the carbon source is one or more of glucose, sucrose, glycerol and fructose.
Preferably, the Basal medium comprises one or more of Basal, BBM and BG 11.
Preferably, the basal medium is a nitrogen-deficient basal medium.
Preferably, the microalgae is selected from one or more of chlorella, scenedesmus and chrysophyceae.
Preferably, the chlorella is chlorella pyrenoidosa.
Preferably, the initial concentration of the inoculated microalgae is 106-109one/mL, preferably 107-108one/mL.
Preferably, the conditions of the culture include: culturing at 10-50 deg.C for 3-20 days with illumination intensity of 0-400 μmol s -1m-2The pH value is 5-9.
Preferably, the conditions of the culture include: the illumination intensity is 145-255 mu mol s-1m-2。
Preferably, the microalgae are inoculated after activated culture.
Preferably, the conditions of the activation culture include: the illumination intensity is 0-400 mu mol s-1m-2The initial carbon source concentration is 0-50g/L, the nitrogen source concentration is 3-20g/L, the pH value is 5-8, the culture temperature is 10-50 ℃, and the culture time is 40-300 hours.
Preferably, the initial carbon source concentration of the activation culture is 10-30 g/L.
The method provided by the invention can remove ammonia nitrogen in the wastewater, purify the water and simultaneously realize the accumulation of microalgae biomass, can effectively reduce the treatment cost of the wastewater, realizes the comprehensive utilization of resources, has economic and environmental-friendly double values, is suitable for the purification treatment of the wastewater in large, medium and small chemical plants, and is particularly suitable for large-scale enterprises with high-yield and high-ammonia nitrogen wastewater.
Drawings
FIG. 1 is a graph showing the change in dry weight during culture at different initial inoculum concentrations in example 1;
FIG. 2 is a graph showing the variation of ammonia nitrogen content during the culture in example 1 at different initial inoculation concentrations;
FIG. 3 is a graph showing the pigment content after the end of the culture at different initial inoculation concentrations in example 1;
FIG. 4 is a graph showing the change in ammonia nitrogen content during the culture at different initial glucose concentrations in example 2;
FIG. 5 is a graph showing the change in dry weight during culture at different initial glucose concentrations in example 2;
FIG. 6 is a graph showing the pigment content after the completion of the culture at different initial glucose concentrations in example 2;
FIG. 7 is a graph showing the protein content after the end of the culture in example 2 at different initial glucose concentrations;
FIG. 8 is a graph showing the change in the number of cells during the culture under different light intensities in example 3.
Detailed Description
The following describes in detail specific embodiments of the present invention. It should be understood that the detailed description and specific examples, while indicating the present invention, are given by way of illustration and explanation only, not limitation.
The invention provides a method for treating molecular sieve wastewater by using microalgae, which inoculates the microalgae in the molecular sieve wastewater containing a basic culture medium for culture.
According to the method, the pollutants in the wastewater are effectively removed as nutrient substances required by the propagation of the algae through the growth of the microalgae in the wastewater containing a basic culture medium, the method is efficient, energy-saving and environment-friendly, the accumulation of the microalgae is realized while ammonia nitrogen in the wastewater is removed and the water quality is purified, the treatment cost of the wastewater is effectively reduced, the comprehensive development and utilization of resources are realized, the method has economic and environment-friendly double values, and is suitable for the purification treatment of the wastewater in large and medium-sized chemical plants, especially suitable for large-scale enterprises with high ammonia nitrogen content.
The molecular sieve wastewater is not particularly limited, and mainly refers to wastewater generated in a molecular sieve synthesis process, particularly wastewater with high ammonia nitrogen concentration, and the type of the molecular sieve is not particularly limited, and NaY molecular sieve wastewater is preferably treated in the present invention.
According to the invention, the salinity of the molecular sieve wastewater is preferably 10-40mg/L, NH4 +1000-5000mg/L, PO4 3-0-300mg/L, Mn2+、Ca2+、K+、Cu2+、Zn2+、Mg2+、Fe2+Each is 0-200 mg/L.
According to the invention, in order to meet the requirement of microalgae growth and increase the accumulation of microalgae biomass, a carbon source is preferably additionally added into the molecular sieve wastewater.
The carbon source is not particularly limited, and may be one or more of glucose, sucrose, glycerol, fructose, cassava residue hydrolysate, molasses, sugar-containing wastewater, and the like, and in the present invention, it is preferable that the carbon source is one or more of glucose, sucrose, glycerol, and fructose.
According to the invention, in order to further meet the requirement of microalgae growth and increase the accumulation of microalgae biomass, the concentration of the carbon source is preferably 0-50g/L, and more preferably 1-20 g/L.
According to the invention, it is preferred that the Basal medium comprises one or more of Basal, BBM and BG 11.
According to the invention, in order to further effectively remove ammonia nitrogen, the basic culture medium is preferably a nitrogen-deficient basic culture medium. By selecting nitrogen-deficient inlet and outlet culture media, the microalgae can completely utilize the nitrogen source in the wastewater to realize effective denitrification.
According to the present invention, preferably, the microalgae is selected from one or more of chlorella, scenedesmus and chrysophyceae.
According to the present invention, preferably, the chlorella is chlorella pyrenoidosa.
In the present invention, in order to achieve effective ammonia nitrogen removal, the initial concentration of the inoculated microalgae is preferably 106-109one/mL, more preferably 107-108one/mL.
According to the present invention, in order to further achieve effective ammonia nitrogen removal, preferably, the culture conditions include: culturing at 10-50 deg.C for 3-20 days with illumination intensity of 0-400 μmol s-1m-2The pH value is 5-9; more preferably, the conditions of the culture include: the culture temperature is 25-35 ℃, the culture time is 5-18 days, the illumination intensity is 145--1m-2The pH value is 6-8.
The light source is not particularly limited, and includes, but is not limited to, a fluorescent lamp, an LED lamp, a fluorescent lamp, and the like.
According to the invention, in order to further effectively remove ammonia nitrogen, the microalgae is preferably inoculated after being subjected to activated culture.
According to the present invention, preferably, the conditions of the activation culture include: the illumination intensity is 0-400 mu mols-1m-2The initial carbon source concentration is 0-50g/L, the nitrogen source concentration is 3-20g/L, the pH value is 5-8, the culture temperature is 10-50 ℃,the culture time is 40-300 hours; more preferably, the conditions of the activation culture include: the illumination intensity is 100--1m-2The initial carbon source concentration is 10-30g/L, the nitrogen source concentration is 8-15g/L, the pH value is 6-7, the culture temperature is 25-35 ℃, and the culture time is 150-250 hours.
The activation culture is not particularly limited, and may be an autotrophic culture or a mixotrophic culture, and when an autotrophic culture is used, the carbon source concentration is preferably 0 g/L.
In the method of the present invention, preferably, polyculture culture is employed; more preferably, the initial carbon source concentration of the activation culture is 10-30 g/L.
The method provided by the invention can remove ammonia nitrogen in the wastewater, purify the water quality and simultaneously realize the accumulation of microalgae biomass, can effectively reduce the treatment cost of the wastewater, realizes the comprehensive development and utilization of resources, has double values of economy and environmental protection, is suitable for the purification treatment of the wastewater quality of large, medium and small chemical plants, and is particularly suitable for large-scale enterprises with high-yield and high-ammonia nitrogen wastewater.
The present invention will be described in more detail with reference to the following examples, but the present invention is not limited to the following examples.
The measuring method comprises the following steps:
1. NH in wastewater3Determination of the-N content
The measurement was carried out using a multi-parameter water quality analyzer, HANNA HI83200, italy. And selecting a proper range, diluting the sample to be detected to the measuring range, adding corresponding reagents into the cuvette according to the instrument instruction, measuring and reading. After the reading is finished, the reading is multiplied by the dilution factor to obtain NH in the culture medium3-the content of N.
2. Measurement of removal Rate (%)
The ammonia nitrogen removal rate is [ (ammonia nitrogen content before sample treatment-ammonia nitrogen content of sample after microalgae treatment)/ammonia nitrogen content before sample treatment ] × 100%.
3. Determination of the Dry weight
And (3) centrifuging the culture solution at a high speed to remove the supernatant, resuspending the obtained algae mud with deionized water, centrifuging at a low speed (3000rpm) for 5 minutes, removing the suspended supernatant with bacteria, and repeatedly centrifuging until the supernatant is colorless and transparent. The algal puree was transferred to a weighed 10mL centrifuge tube, centrifuged at high speed (12000rpm) for 5 minutes, the supernatant removed, placed in a 60 ℃ oven to dry and weighed.
4. Determination of cell number
The flow cytometer of Beckmann corporation, U.S. model number cytoflex-AW442P4, was used, and the sample flow rate was optimized for best detection, and was set to 14-100. mu.L/min. The cell rate of the sample is 2500 events/sec or less, which may affect the accuracy of the measurement. During the sample measurement, the fixed flow rate can be set at 35 μ L/min to ensure the accuracy of the experiment. In terms of setting the detection Threshold (Threshold), the peak height parameter (FSC-H) of the forward angle scattered light is set to 80000. In the setting of the color Compensation (SetColor Compensation), it is possible to set the Compensation values to be all 0%. In the aspect of sample injection control (Run Settings), the number of cells (Events), the sample injection Time (Time) and the sample injection volume (Volumn) can be respectively selected for setting, so that the number of target cells to be finally detected is ensured to be more than 10000. Before analysis, the sample to be tested adopts the rotation speed of 8000rpm to separate and collect the algae cells in the chlorella culture solution or suspension, then adopts deionized water or phosphate buffer concentration (10mM, pH 7.4) to suspend, and repeats twice and then suspends again. The obtained heavy suspension has cell density of Chlorella of 6 × 10 5-8×106CFU/mL (one/mL), on one hand, the sample introduction requirement of the flow cytometer is satisfied (the number of cells or particles per mL is 1X 10)3-5×106) On the other hand, too high or too low cell density affects the accurate determination of fluorescence. And finally, filtering the cell suspension to be detected by adopting a 40-micron water-phase filter membrane, placing the filtered cell suspension in a 2mL centrifuge tube for sample detection, and shaking up before sample injection to ensure the accuracy of a sample detection result. When the cytoflex-AW442P4 flow cytometer detects the number of cells in a sample and fluorescence values, the number of the cells and the average fluorescence intensity of the cells in the sample under FITC and APC-A, PC 5.5.5-A channels are respectively detected. Then creating a histogram of fluorescence channels SSC and APC-A, and automatically calculating chlorella by flow cytometryMean fluorescence intensity of chlorophyll in cells.
5. Determination of the pH value
The pH of the sample at room temperature 25 ℃ was determined using a pH meter.
6. Method for measuring chlorophyll and protein content
6.1 measurement of pigment content
Accurately weighing 20mg of freeze-dried algae powder in a freezing tube by using an electronic balance, adding 800 mu L of 90% acetone, adding a proper amount of ceramic beads, and performing cell wall breaking treatment. Cooling in liquid nitrogen, breaking cell wall, and repeating for three times until the ceramic beads are white. Collecting the supernatant in a 10mL screw test tube, adding 90% acetone to a constant volume of 10mL, uniformly blowing and sucking by using a pipette, centrifuging at 6000rpm and 4 ℃ for 5min, taking the supernatant to a constant volume of 10mL, taking a proper amount of sample to dilute to a proper multiple, taking 90% acetone solution as a blank, measuring the absorbance of the sample at 470nm, 646nm and 663nm in an ultraviolet visible spectrophotometer, and calculating by the following formula:
Ca＝12.21×A663－2.81×A646
Cb＝20.13×A646－5.03×A663
Ct＝(1000×A470－3.27×Ca－104×Cb)/198
Wherein, CaIs chlorophyll a, CbIs chlorophyll b, CtRefers to total carotenoids in units of μ g/mL.
6.2 determination of protein content
The protein content was determined using a semi-automatic Kjeldahl apparatus from FOSS. And (4) converting the nitrogen content in the sample to obtain the protein content in the algae powder.
In the examples described below, the microalgae used were Chlorella pyrenoidosa (Chlorella pyrenoidosa) numbered SJTU-2, which was offered by professor Chenfeng, Beijing university.
The molecular sieve wastewater is NaY molecular sieve wastewater, wherein the salinity is 38mg/L, NH4 +Is 3850mg/L, PO4 3-Is 0mg/L, Mn2+Is 0mg/L, Ca2+21.1mg/L,K+Is 62mg/L, Cu2+Is 0mg/L, Zn2+Is 0mg/L, Mg2+Is 7.88g/L, Fe2+Is 0 mg/L.
Example 1
Step 1: activated algae seed and its seed liquid preparation
Transferring the Chlorella pyrenoidosa SJTU-2 strain stored at-60 deg.C to the slant of basal culture medium containing 10g/L glucose, culturing at 28 deg.C under illumination of 50 μmol m-2s-1And (5) when the plate culture medium grows single algae colonies.
Inoculating Chlorella pyrenoidosa single colony into basal liquid culture medium containing 10g/L glucose with inoculating loop, and irradiating at 30 deg.C under 100 μmol s-1m-2The seed liquid was used as the seed liquid after 5 days of cultivation in a constant temperature shaking table. The composition of the basal medium (pH 6.1) is shown in Table 1 below (in mg/L). Centrifuging the chlorella pyrenoidosa seed solution in logarithmic growth phase at 8000rpm for 3min, discarding the upper culture medium, resuspending with sterile water, centrifuging again to remove the supernatant, and inoculating the collected chlorella pyrenoidosa cells.
TABLE 1
Content (c) of Components Components Content (wt.) Components Content (wt.)
1000 Glucose NaNO3 1250 K2HPO4 1250
1000 MgSO4·7H2O EDTA 500 H3BO3 114.2
111 CaCl2 FeSO4·7H2O 4.98 ZnSO4·7H2O 8.82
1.42 MnCl2·4H2O NaMoO4·2H2O 1.19 CuSO4·5H2O 1.57
0.49 Co(NO3)2·6H2O pH 6.1 / /
Step 2: placing 500mL of NaY molecular sieve wastewater on a magnetic stirrer for three times of dilution, sequentially adding basal mother liquor, uniformly stirring, adjusting the pH to 6.1, and finally adding glucose according to the final concentration of 10 g/L. Subpackaging into 250mL conical bottles with liquid loading of 100mL, sealing with sealing film, placing into autoclave, and sterilizing at 115 deg.C for 15 min. After the sterilization is finished, the shake flask is taken out and cooled to the room temperature.
Collecting Chlorella pyrenoidosa cells, and counting according to cell number of 1 × 106、5×106、1×107、5×107、1×108Inoculating into the culture medium containing NaY molecular sieve wastewater prepared in the step 2 under aseptic conditions. The culture conditions were: the rotating speed is 150r/m, the temperature is 30 ℃, the light source adopts the LED hard lamp strips of positive white light to be connected in series and arranged side by side, and the illumination intensity is 150 +/-10 mu mol s-1m-2The culture time was 6 days. And after the culture is finished, centrifugally collecting cells, centrifugally washing for multiple times, collecting algae mud, carrying out vacuum freeze-drying to obtain algae powder, measuring the dry weight change and ammonia nitrogen variation of the algae powder, and analyzing the chlorophyll and protein contents in the algae powder, wherein the results are shown in table 2 and figures 1-3.
TABLE 2
Initial inoculum concentration (one/mL) 1×106 5×106 1×107 5×107 1×108
Removal Rate (%) 34.7±0.5 57.5±0.14 64.2±0.13 75.3±0.01 83.3±0.01
NH4 +Reduction (mg/L) 427±6.7 708±12.2 790±15.7 928±9.6 1026±9.7
NH4 +Decrease average Rate () 71.16±2.3 118±2 1.61±1.61 131.6±0.28 171±6.1
As can be seen from table 2 and fig. 1 to 3: the inoculation amount is 1 multiplied by 10 6-1×108The ammonia nitrogen removal rate at the time of each mL is 34.7-83.3%, the ammonia nitrogen consumption rate is 71.16-171mg/L/D, and the dry weight content of the chlorella pyrenoidosa reaches 9.5-25.1 g/L.
In the embodiment, after the NaY molecular sieve wastewater is treated by the microalgae, the content of COD and total phosphorus in the wastewater can be reduced while the solid content and the chromaticity in the wastewater are reduced.
Example 2
Inoculation was performed using chlorella pyrenoidosa cells collected in step 1 of example 1.
Placing 500mL of NaY molecular sieve wastewater on a magnetic stirrer for three times of dilution, sequentially adding basal mother liquor, uniformly stirring, adjusting the pH to 6.1, and finally adding glucose according to final concentrations of 10g/L, 20g/L, 30g/L, 40g/L and 50 g/L. Subpackaging into 250mL conical bottles with liquid loading of 100mL, sealing with sealing film, placing into autoclave, and sterilizing at 115 deg.C for 15 min. Taking out the shake flask after the sterilization, cooling to room temperature, inoculating 1X 106The culture conditions of the chlorella pyrenoidosa cells per mL are as follows: the rotating speed is 150rpm, the temperature is 30 ℃, the light source adopts the LED hard lamp strips of positive white light to be connected in series and placed side by side, and the illumination intensity is 150 +/-10 mu mol s-1m-2The incubation time was 12 hours. And after the culture is finished, centrifugally collecting cells, centrifugally washing for multiple times, collecting algae mud, carrying out vacuum freeze-drying to obtain algae powder, measuring the dry weight change and ammonia nitrogen variation of the algae powder, and analyzing the chlorophyll and protein contents in the algae powder, wherein the results are shown in table 3 and figures 4-7.
TABLE 3
Figure BDA0001847482940000101
As can be seen from Table 3 and FIGS. 4-7: when the initial glucose concentration is respectively 10-50g/L, the ammonia nitrogen removal rate is 9.1-33.2%, the ammonia nitrogen consumption rate is 62.8-222.9mg/L/D, the removal rate is 9.1-33.2%, the dry weight content of the chlorella pyrenoidosa reaches 10.2-17.8g/L, and the protein content reaches 32-44%.
Example 3
The chlorella pyrenoidosa cells collected in step 1 of example 1 were used for inoculation.
The wastewater pretreatment and inoculation were the same as in example 2, except that: after the inoculation is finished, the shake flasks are respectively placed in 45-55 mu mol s-1m-2、95-105μmol s-1m-2、145-155μmol s-1m-2、195-205μmol s-1m-2、250-255μmol s-1m-2Under the illumination intensity of the light source for 156 hours, each gradient adopts threeParallel samples. The test results are shown in table 4 and fig. 8.
TABLE 4
Figure BDA0001847482940000111
As can be seen from table 4 and fig. 8: the illumination intensity is 45-255 mu mol s-1m-2The ammonia nitrogen removal rate is 40.6-42.8%, the ammonia nitrogen consumption rate is 199.2-210mg/L/D, and the cell number of Chlorella pyrenoidosa is 3 × 108one/mL.
The method for efficiently assimilating ammonia nitrogen by using microalgae can realize the accumulation of microalgae while purifying the wastewater, effectively reduce the treatment cost of the wastewater, realize the comprehensive development and utilization of resources, have economic and environmental-friendly double values, are suitable for the purification treatment of the wastewater in large, medium and small chemical plants, and are very suitable for being popularized in large-scale enterprises with high-yield and high-ammonia nitrogen wastewater.
The preferred embodiments of the present invention have been described in detail above with reference to the accompanying drawings, but the present invention is not limited thereto. Within the scope of the technical idea of the invention, numerous simple modifications can be made to the technical solution of the invention, including combinations of the individual specific technical features in any suitable way. The invention is not described in detail in order to avoid unnecessary repetition. Such simple modifications and combinations should also be considered as disclosed in the present invention, and all such modifications and combinations are intended to be included within the scope of the present invention.

Claims (9)

1. A method for removing ammonia nitrogen in molecular sieve wastewater by using microalgae is characterized in that the microalgae subjected to activation culture is inoculated into the molecular sieve wastewater containing a basic culture medium for culture,
wherein the salinity of the molecular sieve wastewater is 10-40 mg/L and NH4 +1000-5000 mg/L, PO4 3-0-300 mg/L, Mn2+、Ca2+、K+、Cu2+、Zn2+、Mg2+、Fe2+Each is 0-200 mg/L；
The initial concentration of the inoculated microalgae is 107-108Per mL;
the conditions of the activation culture include: the illumination intensity is 50-400 mu mol s-1m-2The initial carbon source concentration is 10-50 g/L, the nitrogen source concentration is 3-20 g/L, the pH value is 5-8, the culture temperature is 10-50 ℃, and the culture time is 40-300 hours;
The conditions of the culture include: culturing at 10-50 deg.C for 3-20 days with illumination intensity of 45-255 μmol s-1m-2The pH value is 5-9;
the microalgae is Chlorella pyrenoidosa.
2. The method of claim 1, wherein the molecular sieve wastewater is further supplemented with a carbon source.
3. The method of claim 2, wherein the concentration of the supplemental carbon source is 0-50 g/L.
4. The method of claim 3, wherein the concentration of the supplemental carbon source is 1-20 g/L.
5. The method of claim 2, wherein the carbon source is one or more of glucose, sucrose, glycerol, and fructose.
6. The method of claim 1, wherein the Basal medium comprises one or more of Basal, BBM, and BG 11.
7. The method of claim 6, wherein the basal medium is a nitrogen deficient basal medium.
8. The method of claim 1, wherein the conditions of the culturing comprise: the illumination intensity is 145-255 mu mol s-1m-2。
9. The method of claim 1, wherein the initial carbon source concentration of the activation culture is 10-30 g/L.
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