CN111094565B - 指导核酸的产生和用途 - Google Patents

指导核酸的产生和用途 Download PDF

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Publication number
CN111094565B
CN111094565B CN201880051065.5A CN201880051065A CN111094565B CN 111094565 B CN111094565 B CN 111094565B CN 201880051065 A CN201880051065 A CN 201880051065A CN 111094565 B CN111094565 B CN 111094565B
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nucleic acid
sequence
dna
site
pam
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CN111094565A (zh
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S·B·古尔格钦
M·L·卡彭特
M·拉斯穆森
S·拉德哈基什南
A·K·埃尔默
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ARC Bio LLC
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ARC Bio LLC
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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/12—Applications; Uses in screening processes in functional genomics, i.e. for the determination of gene function
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C12N2330/31—Libraries, arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201880051065.5A 2017-06-07 2018-06-07 指导核酸的产生和用途 Active CN111094565B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201762516619P 2017-06-07 2017-06-07
US62/516,619 2017-06-07
US201762548036P 2017-08-21 2017-08-21
US62/548,036 2017-08-21
PCT/US2018/036563 WO2018227025A1 (en) 2017-06-07 2018-06-07 Creation and use of guide nucleic acids

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CN111094565A CN111094565A (zh) 2020-05-01
CN111094565B true CN111094565B (zh) 2024-02-06

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US (2) US20200190508A1 (https=)
EP (1) EP3635114B1 (https=)
JP (1) JP7282692B2 (https=)
CN (1) CN111094565B (https=)
AU (1) AU2018279112B2 (https=)
CA (1) CA3065384A1 (https=)
WO (1) WO2018227025A1 (https=)

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AU2016365720B2 (en) 2015-12-07 2020-11-26 Arc Bio, Llc Methods and compositions for the making and using of guide nucleic acids
EP3861135B1 (en) 2018-10-04 2023-08-02 Arc Bio, LLC Normalization controls for managing low sample inputs in next generation sequencing
CA3120359A1 (en) * 2018-11-19 2020-05-28 The Regents Of The University Of California Methods for detecting and sequencing a target nucleic acid
CA3136228A1 (en) 2019-04-09 2020-10-15 Arc Bio, Llc Compositions and methods for nucleotide modification-based depletion
WO2021142197A1 (en) * 2020-01-09 2021-07-15 Duke University Composistions and methods for crispr enabled dna synthesis
AU2021246531A1 (en) * 2020-04-02 2022-11-24 Altius Institute For Biomedical Sciences Methods, compositions, and kits for identifying regions of genomic DNA bound to a protein
CA3182741A1 (en) * 2020-06-15 2021-12-23 Aziz AL'KHAFAJI Chimeric amplicon array sequencing
WO2022148450A1 (en) * 2021-01-08 2022-07-14 Wuhan University Compositions and methods for instant nucleic acid detection
CN113462755B (zh) * 2021-05-06 2023-03-21 中国人民解放军陆军军医大学第一附属医院 一种用于短链非编码rna检测的模块化酶电路检测系统
CN114293264B (zh) * 2021-12-21 2024-12-27 翌圣生物科技(上海)股份有限公司 酶法靶序列随机sgRNA文库的制备方法
WO2023158739A2 (en) * 2022-02-17 2023-08-24 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
CN119120659B (zh) * 2023-07-13 2025-07-25 上海市第十人民医院 一种基于切刻内切酶的一锅式多靶标正交检测系统及其应用

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EP3635114C0 (en) 2024-11-06
EP3635114A4 (en) 2021-03-17
CN111094565A (zh) 2020-05-01
AU2018279112A1 (en) 2019-12-19
JP7282692B2 (ja) 2023-05-29
WO2018227025A1 (en) 2018-12-13
CA3065384A1 (en) 2018-12-13
US20250179477A1 (en) 2025-06-05
JP2020524491A (ja) 2020-08-20
US20200190508A1 (en) 2020-06-18
EP3635114A1 (en) 2020-04-15
EP3635114B1 (en) 2024-11-06
AU2018279112B2 (en) 2024-11-07

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