CN111084788A

CN111084788A - Artificial intercellular substance

Info

Publication number: CN111084788A
Application number: CN201811235590.0A
Authority: CN
Inventors: 马雪松; 顾苏兰
Original assignee: Shanghai Lan'ang Electronic Technology Development Co ltd
Current assignee: Shanghai Lan'ang Electronic Technology Development Co ltd
Priority date: 2018-10-22
Filing date: 2018-10-22
Publication date: 2020-05-01

Abstract

The invention relates to an artificial intercellular substance, belonging to the field of biological medicine. The invention uses artificial simulation or manufacturing 'artificial intercellular substance' with the same or similar function and physical index as the 'intercellular substance' of healthy organism, or uses technical means to collect the 'natural intercellular substance' with normal function in the fresh remains of healthy organism. So long as a living body's "intercellular substance" is not used, it is within the scope of the present invention. The invention avoids slaughtering living organisms, protects lives, and provides an environment-friendly, cheap and fully-supplied 'intercellular substance' source for modern biology.

Description

Artificial intercellular substance

Technical Field

The invention relates to an artificial intercellular substance, belonging to the field of biological medicine.

Background

The smallest constituent unit of a multicellular organism is a cell, and the cell clusters are recombined into various tissues to finally form the organism. In fact, however, there is another essential component of the organism, namely the "intercellular substance", which exists between the cell membrane and the cells and is a substance produced by the cells without cellular morphology and structure. The material and energy exchange between cells depends on the intercellular substance. The composition of the intercellular substance varies from species to species, such as the "intercellular substance" of mammals, including fibers, matrix, and fluid substances (interstitial fluid, lymph fluid, plasma, etc.).

The 'intercellular substance' has great application value in the fields of scientific research, biological medicine and the like.

Disclosure of Invention

The content of the invention is to artificially simulate or produce an artificial intercellular substance with the same or similar function and physical indexes as those of a healthy organism, or collect a natural intercellular substance with normal function in a fresh remains of the healthy organism by using a technical means.

The invention solves the traditional idea of obtaining the intercellular substance by using living organisms, avoids butchering and killing the living organisms, and provides an environment-friendly, cheap and fully-supplied intercellular substance source for modern biology.

The inventor finds that the main components of the intercellular substance are divided into fiber, matrix and fluid substances. The intercellular substance is also a solution in nature and thus can be divided into two parts, a solvent and a solute. The solvent is water, and is water treated by the cells themselves. The solute is complex in composition, but mainly comprises three types, namely salt, cellulose and protein, and also comprises components with extremely low content such as carbon dioxide and oxygen dissolved in water. The fiber components referred by the inventor comprise fibers and semi-fiber substances, and the protein comprises polypeptide, amino acid, macromolecular protein and the like.

The inventors have found that some components can be simplified, naturally produced or even omitted if the intercellular substance is artificially prepared. Such as carbon dioxide, etc. dissolved in water, if the water for preparation or the finished product is exposed to the air for a period of time, the corresponding gas component will be dissolved in the water or the finished product naturally, and no special addition is needed. Among protein components, macromolecular proteins are certainly necessary, polypeptides can be simplified into one or more common types, and fibers can be omitted. Practical application shows that the principle does not influence the application effect and the support of cell functions.

The inventor determines the kind of raw materials and the key preparation process for artificially preparing the intercellular substance through a large number of experiments.

The solvent is selected from water, but higher purity is required, distilled water can be adopted, but triple distilled water is most suitable and can be easily obtained under the existing industrial technology. The salts mainly play a role in ion balance and substance supplement, so sodium, potassium, calcium ions and salts thereof are necessary, other trace ions such as iron, magnesium, zinc, chlorine, hydroxide radical and the like can be added in a proper amount, and when the salt is used in vitro, only the main species can be added to meet the requirement. Experiments prove that the salts can adopt sodium chloride, potassium chloride, calcium chloride, zinc gluconate, ferrous lactate and the like, and no extra hydroxide is needed. The inventor also finds that if salt components are required to be enriched as much as possible, plant water extracts such as water extracts of ginkgo, arbutin, lily and the like can be selected, and the effect is better. The protein can be selected from collagen, hyaluronic acid and the like, if the plant water extract is added, the protein (the invention also comprises polypeptide, amino acid and the like) is more abundant, and the cost can be saved, the preparation process can be simplified and the use effect can be improved.

Of course, the artificial preparation method, regardless of the abundance of the components and the perfection of the functions, is inevitably inferior to the natural intercellular substance. Thus, the inventors have also identified a method for extracting the intercellular substance from a freshly dead organism.

First, the death time of the organism should be within one hour. Secondly, the invention is primarily intended for human use, and therefore the biological species is mammalian in nature, but other species of organisms may be used to extract the intercellular substance in a similar manner.

In the case of mammals, not all parts are suitable for extraction, such as bone, nervous system, digestive system, etc., which are not suitable. Suitable sites are muscle tissue and joint cavities. After the fresh muscle tissue is stripped from the body, the tissue is cut into slices of 2-10 microns by an ultrathin blade for pathological section, and the slices are optimally cut in the direction perpendicular to the muscle fibers. Then, the medium-speed filter paper is horizontally paved up and down along the section and is placed in a Buchner funnel, preferably, only one filter paper is used, and the extraction effect can be influenced by the superposition of a plurality of filter papers. The filter paper is lightly pressed with a medical grade plastic plate or a suitable instrument with a force to press the liquid without crushing the slices. Finally, the extract is extracted and stored at 4 ℃.

The artificial intercellular substance has positive social benefit and economic benefit, not only protects life, is green and environment-friendly, but also has cost far lower than that of the intercellular substance of living organisms.

It is clear that it is within the scope of the invention to use "intercellular substance" of the living body, as long as it is not used. The method for producing the "artificial intercellular substance" is many and not limited to the examples.

Detailed Description

1) The preparation process of "artificial intercellular substance" used in vitro.

The solvent is selected from triple distilled water. And (3) standing the triple distilled water in a sterile environment for 1 hour to dissolve the water-soluble gas components in the air into the triple distilled water. Then, the salts are fed first. The salts comprise sodium chloride (content: 0.85%), potassium chloride (content: 0.5%), calcium chloride (content: 0.2%), folium Ginkgo water extract (content: 0.01%), and Bulbus Lilii water extract (content: 0.005%). After stirring and dissolving, the protein component is fed. Comprises collagen (content: 0.1%), sodium hyaluronate (0.05%). And (4) stirring uniformly. Standing for 24 hr, and stirring to completely homogenize. Storing at 4 deg.C. The preservation period is 4 weeks. If the concentration of sodium chloride is increased to 1.5% or more, the finished product can be stored at normal temperature for at least 6 months.

2) A process for extracting natural intercellular substance.

A fresh remains of a healthy organism whose death has been confirmed is prepared by collecting a clean muscle tissue and cutting the muscle tissue into small pieces of about 5cm by 5 cm. And cutting into slices with a cross section of about 2 microns by using an ultrathin blade for the sterilized pathological section in a direction perpendicular to muscle fibers. And (3) clamping the slices between two pieces of filter paper by using two sterilized medium-speed filter paper slightly larger than the section to form a three-layer structure. The three-layer structure is placed in a medium Buchner funnel, and the filter paper is lightly pressed by using a sterilized dry PP plastic sheet or a sterilized glass pestle, wherein the force is not required to crush the slices, but the liquid is preferably leaked out. The fluid is the intercellular substance. After the liquid is leaked out to the maximum extent, the liquid is sucked in a closed manner from the outlet end of the Buchner funnel by a disposable syringe, and the intercellular substance is sucked and collected. Then stored at 4 ℃. The process can be repeated continuously for many times, and a large amount of natural intercellular substance can be effectively collected. The whole extraction process is preferably completed within 10-20 minutes. If low temperature operating conditions are present, the time can be extended to 1 hour.

Claims

1. An artificial intercellular substance is characterized by being artificially simulated or manufactured.

2. An artificial intercellular substance characterized in that each index is the same as or similar to that of a healthy organism.

3. An artificial intercellular substance is characterized in that a functional natural intercellular substance can be collected.

4. An "artificial intercellular substance" characterized in that the collection subject is not a living body.

5. An artificial intercellular substance is characterized in that the collection object is selected from fresh remains of healthy organisms.