CN111084297A - Feed additive for preventing and treating enteritis of pikes - Google Patents

Feed additive for preventing and treating enteritis of pikes Download PDF

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Publication number
CN111084297A
CN111084297A CN201911320825.0A CN201911320825A CN111084297A CN 111084297 A CN111084297 A CN 111084297A CN 201911320825 A CN201911320825 A CN 201911320825A CN 111084297 A CN111084297 A CN 111084297A
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parts
fermentation
preventing
feed additive
pikes
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刘学成
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Anhui Shengmi Breeding Co Ltd
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Anhui Shengmi Breeding Co Ltd
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Priority to CN201911320825.0A priority Critical patent/CN111084297A/en
Publication of CN111084297A publication Critical patent/CN111084297A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/153Nucleic acids; Hydrolysis products or derivatives thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/28Silicates, e.g. perlites, zeolites or bentonites
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/113Acidophilus

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Birds (AREA)
  • Inorganic Chemistry (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Insects & Arthropods (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Sustainable Development (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fodder In General (AREA)

Abstract

The invention provides a feed additive for preventing and treating enteritis of pikes, which comprises the following raw materials in parts by weight: 30-50 parts of fruit enzyme, 10-20 parts of yeast cell wall, 6-12 parts of carboxymethyl polysaccharide, 0.8-1.5 parts of nucleotide and 18-24 parts of carrier; the fruit ferment is mainly prepared from honey pomelo, kiwi fruit, mulberry and banana through fermentation of compound bacteria, wherein the compound bacteria are bacillus subtilis and lactobacillus acidophilus. The feed additive can improve the flora environment of intestinal tract of pike, change the activity of metabolic enzyme, and enhance the nonspecific immunity of fish, thereby effectively preventing and treating enteritis.

Description

Feed additive for preventing and treating enteritis of pikes
Technical Field
The invention belongs to the technical field of feed additives, and particularly relates to a feed additive for preventing and treating enteritis of pikes.
Background
The body of the pike is round and scaly. The dorsal part is grey, the ventral part is light grey, and the two lateral scales have black vertical lines. It is a kind of offshore fish, which is fond of inhabiting in rivers and gulfs and also enters into fresh water. The water surface mud is lively and jumping, and is often grouped and traced in the countercurrent to eat organic matters in the soil at the water bottom. The body type is large, and the Chinese is produced in south China sea, east China sea, yellow sea and Bohai sea. The body of the mullet is slender, and the largest mullet can be as long as 1.8 meters. The head of the pike is short and wide, and the scales are large. The back of the pike is grey, the ventral surface is light grey, and the scales on the two sides have black vertical lines. The mullets live in coastal mouths and rivers or in salt water. The pike likes the colony life and takes the organic matters in the soil at the bottom of the water as food.
With the gradual popularization of the artificial breeding technology of the pikes, the eating demand of people for the pikes is continuously increased, but with the improvement of the breeding density in recent years, enteritis caused by intestinal punctate aeromonas bacterium is continuously generated. The main performance is as follows: sick fish wander off the pool alone, move slowly, and lose or stop appetite. When the intestinal wall is cut open, the intestinal wall is locally inflamed, and the mucus in the intestine is much and is light yellow; red and swollen anus, and erythema on the intestinal wall. Light pressure on the abdomen causes cream-yellow mucus to flow out of the anus. The diseases are mainly frequently generated in the high-temperature period in summer, and once the diseases occur, the infectivity is strong, and the large-area death and even the failure of the harvest of the pike can be caused. Therefore, the quality, the fullness, the survival rate and the like of the mullets in the high-temperature stage in summer influence the adult fish culture and indirectly influence the culture economic benefit.
The Chinese herbal medicines added into fishes for preventing and treating aquatic diseases are used in a small amount in aquaculture, for example, the Chinese herbal medicines are relatively and more reported for preventing and treating the pike diseases, but the reports on other biological prevention and treatment, particularly on the intestinal tract diseases of the pike in summer are not found yet.
Disclosure of Invention
The feed additive can improve the flora environment of intestinal tracts of the pikes, change the activity of metabolic enzymes and enhance the nonspecific immunity of fish bodies, thereby effectively preventing and treating enteritis.
The technical scheme of the invention is realized as follows:
the feed additive for preventing and treating the enteritis disease of the pikes comprises the following raw materials in parts by weight:
30-50 parts of fruit enzyme, 10-20 parts of yeast cell wall, 6-12 parts of carboxymethyl polysaccharide, 0.8-1.5 parts of nucleotide and 18-24 parts of carrier; the fruit ferment is prepared by mainly taking honey pomelo, kiwi fruit, mulberry and banana as raw materials and fermenting the raw materials by using composite bacteria, wherein the composite bacteria are bacillus subtilis and lactobacillus acidophilus.
Preferably, the fruit ferment is prepared by the following steps:
1) raw material treatment: cleaning honey pomelos, kiwi fruits, mulberries and bananas, respectively smashing, mixing, homogenizing, and adjusting the pH value to 3.5-4.5 to obtain homogenate;
2) fermentation: inoculating lactobacillus acidophilus into the homogenate for fermentation, wherein the fermentation temperature is 28-34 ℃, the fermentation time is 3-6 days, then inoculating bacillus subtilis for continuous fermentation, the fermentation temperature is 25-30 ℃, and the fermentation time is 4-8 days;
3) and (3) filtering and concentrating: filtering the fermentation liquor obtained in the step 2) at low temperature, and then concentrating and drying in vacuum to obtain the fruit ferment.
Preferably, the mass ratio of the honey pomelos, the kiwi fruits, the mulberries and the bananas is 4-6: 2-3: 1.
Preferably, the addition amount of the lactobacillus acidophilus is 1.56-2.45% of the weight of the raw materials, and the addition amount of the bacillus subtilis is 2.32-4.08% of the weight of the raw materials.
Preferably, the carboxymethyl polysaccharide is selected from one or more of carboxymethyl dextran, carboxymethyl chitosan and carboxymethyl chitin.
Preferably, the carrier is medical stone powder.
The preparation method of the feed additive for preventing and treating the enteritis of the pikes comprises the following steps:
the raw materials are weighed according to the proportion and then mixed and stirred evenly.
The yeast cell wall has the functions of enhancing the immunity of animals and adsorbing mycotoxin.
The carboxymethyl polysaccharide has various biological activities of resisting tumor, bacteria, oxidation and radiation, reducing blood sugar and the like.
The nucleotide changes the activity of metabolic enzyme and enhances the nonspecific immunity of the fish body.
The invention has the beneficial effects that:
the fruit enzyme is added into the feed additive for preventing and treating the enteritis of the pike, so that the environment of intestinal flora can be improved, the absorption of nutrient substances by the pike is promoted, and the immunity of the pike is further enhanced through yeast cell walls, carboxymethyl polysaccharide, nucleotide and the like, so that the purpose of preventing and treating the enteritis of the pike is achieved.
Detailed Description
Example 1
The feed additive for preventing and treating the enteritis disease of the pikes comprises the following raw materials in parts by weight:
36 parts of fruit ferment, 14 parts of yeast cell wall, 5 parts of carboxymethyl glucan, 5 parts of carboxymethyl chitosan, 0.9 part of nucleotide and 21 parts of medical stone powder.
The fruit ferment preparation method comprises the following steps:
1) raw material treatment: cleaning honey pomelo, kiwi fruit, mulberry and banana, respectively crushing, mixing, homogenizing, and adjusting pH to 3.8 to obtain homogenate; the mass ratio of the honey pomelos to the kiwi fruits to the mulberries to the bananas is 6:2:3: 1.
2) Fermentation: inoculating lactobacillus acidophilus into the homogenate for fermentation, wherein the fermentation temperature is 30 ℃, the fermentation time is 4 days, then inoculating bacillus subtilis for continuous fermentation, the fermentation temperature is 30 ℃, and the fermentation time is 6 days; the addition amount of lactobacillus acidophilus is 2.45% of the weight of the raw material, and the addition amount of bacillus subtilis is 3.28% of the weight of the raw material.
3) And (3) filtering and concentrating: filtering the fermentation liquor obtained in the step 2) at low temperature, and then concentrating and drying in vacuum to obtain the fruit ferment.
The carboxymethyl polysaccharide is one or more selected from carboxymethyl dextran, carboxymethyl chitosan and carboxymethyl chitin.
Example 2
The feed additive for preventing and treating the enteritis disease of the pikes comprises the following raw materials in parts by weight:
50 parts of fruit enzyme, 10 parts of yeast cell wall, 12 parts of carboxymethyl chitin, 1.5 parts of nucleotide and 24 parts of medical stone powder.
The fruit ferment preparation method comprises the following steps:
1) raw material treatment: cleaning honey pomelo, kiwi fruit, mulberry and banana, respectively crushing, mixing, homogenizing, and adjusting pH to 3.5 to obtain homogenate; the mass ratio of the honey pomelos to the kiwi fruits to the mulberries to the bananas is 4:3:2: 1.
2) Fermentation: inoculating lactobacillus acidophilus into the homogenate for fermentation, wherein the fermentation temperature is 28 ℃, the fermentation time is 6 days, then inoculating bacillus subtilis for continuous fermentation, the fermentation temperature is 25 ℃, and the fermentation time is 4-8 days; the addition amount of lactobacillus acidophilus is 1.98% of the weight of the raw material, and the addition amount of bacillus subtilis is 2.46% of the weight of the raw material.
3) And (3) filtering and concentrating: filtering the fermentation liquor obtained in the step 2) at low temperature, and then concentrating and drying in vacuum to obtain the fruit ferment.
Example 3
The feed additive for preventing and treating the enteritis disease of the pikes comprises the following raw materials in parts by weight:
40 parts of fruit ferment, 20 parts of yeast cell wall, 6 parts of carboxymethyl chitosan, 1.2 parts of nucleotide and 20 parts of medical stone powder.
The fruit ferment preparation method comprises the following steps:
1) raw material treatment: cleaning honey pomelo, kiwi fruit, mulberry and banana, respectively crushing, mixing, homogenizing, and adjusting pH to 4.5 to obtain homogenate; the mass ratio of the honey pomelos to the kiwi fruits to the mulberries to the bananas is 5:2:2: 1.
2) Fermentation: inoculating lactobacillus acidophilus into the homogenate for fermentation, wherein the fermentation temperature is 30 ℃, the fermentation time is 5 days, then inoculating bacillus subtilis for continuous fermentation, the fermentation temperature is 25 ℃, and the fermentation time is 6 days; the addition amount of lactobacillus acidophilus is 1.56% of the weight of the raw material, and the addition amount of bacillus subtilis is 4.08% of the weight of the raw material.
3) And (3) filtering and concentrating: filtering the fermentation liquor obtained in the step 2) at low temperature, and then concentrating and drying in vacuum to obtain the fruit ferment.
Example 4
The feed additive for preventing and treating the enteritis disease of the pikes comprises the following raw materials in parts by weight:
30 parts of fruit ferment, 13 parts of yeast cell wall, 10 parts of carboxymethyl glucan, 0.8 part of nucleotide and 18 parts of medical stone powder.
The fruit ferment preparation method comprises the following steps:
1) raw material treatment: cleaning honey pomelo, kiwi fruit, mulberry and banana, respectively crushing, mixing, homogenizing, and adjusting pH to 4.0 to obtain homogenate; the mass ratio of the honey pomelos to the kiwi fruits to the mulberries to the bananas is 6:3:2: 1.
2) Fermentation: inoculating lactobacillus acidophilus into the homogenate for fermentation, wherein the fermentation temperature is 34 ℃, the fermentation time is 3 days, then inoculating bacillus subtilis for continuous fermentation, the fermentation temperature is 30 ℃, and the fermentation time is 8 days; the addition amount of lactobacillus acidophilus is 2.45% of the weight of the raw material, and the addition amount of bacillus subtilis is 2.32% of the weight of the raw material.
3) And (3) filtering and concentrating: filtering the fermentation liquor obtained in the step 2) at low temperature, and then concentrating and drying in vacuum to obtain the fruit ferment.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (6)

1. The feed additive for preventing and treating enteritis of pikes is characterized by comprising the following raw materials in parts by weight:
30-50 parts of fruit enzyme, 10-20 parts of yeast cell wall, 6-12 parts of carboxymethyl polysaccharide, 0.8-1.5 parts of nucleotide and 18-24 parts of carrier; the fruit ferment is prepared by mainly taking honey pomelo, kiwi fruit, mulberry and banana as raw materials and fermenting the raw materials by using composite bacteria, wherein the composite bacteria are bacillus subtilis and lactobacillus acidophilus.
2. The feed additive for preventing and treating enteritis of pikes according to claim 1, wherein the fruit ferment is prepared by the following steps:
1) raw material treatment: cleaning honey pomelos, kiwi fruits, mulberries and bananas, respectively smashing, mixing, homogenizing, and adjusting the pH value to 3.5-4.5 to obtain homogenate;
2) fermentation: inoculating lactobacillus acidophilus into the homogenate for fermentation, wherein the fermentation temperature is 28-34 ℃, the fermentation time is 3-6 days, then inoculating bacillus subtilis for continuous fermentation, the fermentation temperature is 25-30 ℃, and the fermentation time is 4-8 days;
3) and (3) filtering and concentrating: filtering the fermentation liquor obtained in the step 2) at low temperature, and then concentrating and drying in vacuum to obtain the fruit ferment.
3. The feed additive for preventing and treating enteritis of pikes according to claim 2, wherein the mass ratio of honey pomelos, kiwi fruits, mulberries and bananas is 4-6: 2-3: 1.
4. The feed additive for preventing and treating enteritis of pike according to claim 2, wherein the addition amount of lactobacillus acidophilus is 1.56-2.45% of the weight of the raw materials, and the addition amount of bacillus subtilis is 2.32-4.08% of the weight of the raw materials.
5. The feed additive for preventing and treating enteritis of pike according to claim 1, wherein the carboxymethyl polysaccharide is one or more selected from carboxymethyl dextran, carboxymethyl chitosan and carboxymethyl chitin.
6. The feed additive for preventing and treating enteritis of pikes according to claim 1, wherein the carrier is medical stone powder.
CN201911320825.0A 2019-12-19 2019-12-19 Feed additive for preventing and treating enteritis of pikes Pending CN111084297A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115886158A (en) * 2022-12-13 2023-04-04 珠海市德海生物科技有限公司 Feed additive capable of improving meat quality of sea bass and preparation method and application thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115886158A (en) * 2022-12-13 2023-04-04 珠海市德海生物科技有限公司 Feed additive capable of improving meat quality of sea bass and preparation method and application thereof

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