CN111067944A - Pollen nano porous microsphere and preparation method and application thereof - Google Patents
Pollen nano porous microsphere and preparation method and application thereof Download PDFInfo
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Abstract
The invention belongs to the field of biological materials, and particularly relates to a pollen nano porous microsphere as well as a preparation method and application thereof. The method comprises the steps of pretreating peony pollen to primarily inhibit pollen germination, and then sieving; soaking the sieved pollen in 75-100% by volume of ethanol solution for 5-15 min; centrifuging and discarding the supernatant; then treating for 8-15 min by using a slightly alkaline solution; centrifuging and discarding the supernatant; finally, rinsing and drying are carried out to obtain the hollow natural degradable nano porous microspheres. The preparation method has the advantages of reliable and wide material source and low cost; the preparation process is simple and easy to control; the reagent is safe and reliable, and the environmental friendliness is high; the obtained material has excellent biocompatibility.
Description
Technical Field
The invention belongs to the field of biological materials, and particularly relates to a pollen nano porous microsphere as well as a preparation method and application thereof.
Background
Paeonia (Paeonia L.) Mainly comprises a Paeonia lactiflora group and a Paeonia suffruticosa group, wherein pollen of the Paeonia suffruticosa group ranges from an oblong ellipsoid shape to an ellipsoidal shape, the length of a polar axis ranges from 30.69 to 46.25um, and the length of an equatorial axis ranges from 19.49 to 25.71 um; the polar red ratio is 1.85-2.26; IIIThe strip germination ditch is provided with a ditch film; the surface is provided with decorative holes, the diameters of the meshes are 0.2-1.84 um (Zhangiu, Pong' an, Liqing, Chunrong Gao, Gong Jun Hua, Xie Ling, the research of the ultra-micro structure of partial peony pollen grains [ J]The Sichuan university of agriculture bulletin (4): 466-.
The peony belongs to the peony flower, and peony has a long cultivation history in China, and has the following advantages: the large-scale commercial planting is carried out in the Luoyang and the lotus, the material source is stable, the collected pollen does not damage the flower device main body, and the ornamental value of the flowers is not influenced; the florescence is long, the diameter of the flower organ is large, and the commercial collection of pollen is facilitated; the stamens are numerous, and the pollen yield is high; at the end of the flowering period, the flower receptacle is usually cut off as waste, resulting in waste of resources.
Typically, the main components of the pollen wall are sporopouenin, cellulose and pectin. The sporopouenin has acid and alkali resistance and degradation resistance. Therefore, the wall breaking of most pollen requires harsh conditions, such as strong acid, strong alkali, ultrasound, ultra-fine grinding, etc. The peony pollen has simple processing technology and good effect, and is suitable for preparing natural nano porous microspheres.
The nano porous microsphere material is concerned by people due to the unique structural characteristics of the nano porous microsphere material, such as high adsorbability, large specific surface area, large porosity and low density, and is widely applied to the fields of building materials, manned space flight, energy, environmental protection, biological medicine and the like. The natural nano porous microsphere material is the first choice in the fields of biomedicine, cosmetics and the like due to biodegradability and friendly environmental compatibility, and is used for preparing drug carriers, sustained-release agent carriers and the like.
Disclosure of Invention
In order to overcome the defects and shortcomings of the prior art, the invention aims at providing a preparation method of pollen nano porous microspheres, which has the advantages of reliable and wide material source and low cost; the preparation process is simple and easy to control; the reagent is safe and reliable, and the environmental friendliness is high; the obtained material has excellent biocompatibility.
The invention also aims to provide the pollen nano porous microspheres prepared by the preparation method.
The invention further aims to provide application of the pollen nano porous microspheres.
The purpose of the invention is realized by the following technical scheme:
a preparation method of pollen nano porous microspheres comprises the following steps:
(1) pretreating pollen to primarily inhibit pollen germination, and then sieving;
(2) soaking the sieved pollen in 75-100% by volume of ethanol solution for 5-15 min; centrifuging and discarding the supernatant;
(3) treating the pollen soaked in the ethanol solution in the step (2) for 8-15 min by using a slightly alkaline solution; centrifuging and discarding the supernatant;
(4) rinsing the pollen treated by the slightly alkaline solution in the step (3); then drying to obtain the pollen nano porous microspheres;
the pretreatment mode in the step (1) is preferably at least one of drying, microwave treatment and ray treatment, wherein the purpose of the pretreatment is to inhibit pollen germination, and once the pollen germinates, the pollen content composition changes and is not beneficial to dissolution;
the drying temperature is preferably 60-120 ℃;
the screening mesh number in the step (1) is preferably 200 meshes, and impurities such as filaments and drug walls are removed through screening;
preferably, the pollen in the step (1) is collected by selecting flowers in a normal flowering period of healthy Paeonia (Paeonia drug group and peony group);
the pollen collection mode in the step (1) is preferably as follows: at least one of manual collection, vacuum collection, and biological collection;
the specific operation of manual collection is preferably as follows:
cutting the stamen filaments by using forceps or scissors, and pouring the stamen filaments into a collection bag for collection;
the specific operation of the dust collector is preferably as follows:
placing a collection bag in a dust collector, and using the dust collector to suck the pollen and the anther by aiming at the pistil; this method is not applicable when the anthers are not completely dehisced and pollen is released;
the specific operation of biological collection is preferably as follows:
breeding pollinating insects such as bees in a closed greenhouse environment, and collecting pollen collected by the pollinating insects for later use; the method can avoid pollen mixing and save a great deal of manpower, but has higher cost;
the soaking condition in the step (2) is preferably that 75% of ethanol solution in volume fraction is adopted to soak the sieved pollen for 10 min;
the slightly alkaline solution in the step (3) is preferably 2.0X 10 in concentration－3mol/L ~5.0×10－3mol/L sodium carbonate (Na)2CO3) Solution or pH equivalent to a concentration of 2.0X 10－3mol/L ~5.0×10－3Other alkali solutions of mol/L sodium carbonate solution;
the slightly alkaline solution described in the step (3) is more preferably a solution having a concentration of 2.0X 10－3mol/L~4.0×10－3mol/L sodium carbonate solution or pH equivalent concentration of 2.0X 10－3mol/L~4.0×10－3Other alkali solutions of mol/L sodium carbonate solution;
the slightly alkaline solution in the step (3) is more preferably a solution having a concentration of 2.0X 10－3mol/L sodium carbonate solution;
the time of the slightly alkali treatment in the step (3) is preferably 10 min;
the rinsing method in step (4) is preferably: mixing the pollen treated by the slightly alkaline solution in the step (3) with water for rinsing, centrifuging and discarding the supernatant;
the rinsing frequency in the step (4) is preferably 2-3 times;
the drying mode in the step (4) is preferably natural drying, drying or freeze-drying;
the centrifugation conditions in the steps (2), (3) and (4) are preferably 2500-4000 r/min for 30 s-2 min;
the centrifugation conditions in steps (2), (3) and (4) are more preferably 3000 r/min for 1 min;
a pollen nano porous microsphere is prepared by the preparation method;
the pollen nano porous microsphere is applied to the fields of biological medicine and cosmetics;
the method comprises the steps of pretreating the freshly collected pollen to initially inhibit pollen germination and keep pollen vitality, then further treating the pollen by adopting an ethanol solution to thoroughly kill the pollen and keep the shape and the structure of the pollen complete, and promoting the subsequent micro-alkali treatment effect. And then treating the pollen by using a slightly alkaline solution to completely dissolve out the content in the pollen so as to obtain the complete and hollow pollen nano porous microspheres.
Wherein the concentration of the slightly alkaline solution is critical and is less than 2.0 × 10－3The sodium carbonate solution of mol/L can not degrade the inner wall part of the pollen and dissolve out the content, and the concentration is 2.0 multiplied by 10－3The sodium carbonate solution with mol/L above can partially degrade the inner wall of the pollen and dissolve out the content, but the degradation rate of the inner wall of the pollen gradually increases and collapses along with the increase of the concentration and the prolonging of the time, so that the preparation rate gradually decreases. Among them, sodium carbonate (Na)2CO3) The solution is more than 5.0 × 10－3And (5) mol/L, the pollen completely collapses.
Compared with the prior art, the invention has the following advantages and effects:
(1) the raw material adopted by the invention is peony pollen. Peony flowers are planted in large scale and commercialization at home, especially in the Luoyang city of Henan province and the lotus city of Shandong province, and the sources are stable and reliable. The stamens of the pollen are cut off by using scissors during pollen collection, and the ornamental value of the flowers is not influenced by the residual stamens. In order to avoid the generation of seeds and the consumption of nutrients in large quantities, flowers are generally subjected to pruning and discarding treatment at the end of the flowering period.
(2) The process is simple and easy to control. The tolerance of the peony pollen wall to alkali is far lower than that of other pollen, and the peony pollen wall can be partially degraded by using micro alkali to obtain a product with high yield, wherein the microsphere preparation yield is over 80 percent, and the preparation rate is high.
(3) The reagents used in the method are ethanol and sodium carbonate with extremely low concentration, so that the method has high safety, good environmental friendliness and easy treatment of wastewater, and basically cannot cause harm to the environment.
(4) The microsphere prepared by the invention has excellent biocompatibility due to the natural degradable property. Can be tolerated without any significant symptoms in the mouse toxicity test. The expected cardiovascular and cerebrovascular blockage did not occur at all.
(5) The invention removes most pollen contents, greatly reduces the allergen of the pollen and further improves the safety of the pollen.
Drawings
FIG. 1 is a scanning electron micrograph of pollen after direct cutting without any treatment; wherein, A: magnification 7000 ×, B: 8000X.
FIG. 2 is a scanning electron microscope image of the cut pollen nano-porous microspheres prepared in example 1; wherein, A: magnification 1500 ×, B: magnification 6000 x.
FIG. 3 is a scanning electron micrograph (1000X) of the cut pollen nanoporous microspheres prepared in example 2.
FIG. 4 is a scanning electron micrograph (500X) of the cut pollen nanoporous microspheres obtained in example 3.
FIG. 5 is a scanning electron micrograph (2500X) of the cut pollen nanoporous microspheres prepared in comparative example 1.
FIG. 6 is a scanning electron micrograph (930X) of the cut pollen nanoporous microspheres prepared in comparative example 2.
FIG. 7 is a scanning electron micrograph (2000X) of the cut pollen nanoporous microspheres prepared in comparative example 3.
Detailed Description
The present invention will be described in further detail with reference to examples and drawings, but the present invention is not limited thereto.
Example 1
(1) Selecting flowers in normal flowering phase of healthy peony groups to collect pollen, and specifically cutting stamen filaments by using tweezers or scissors, and pouring the cut stamen filaments into a collection bag for collection; drying the collected pollen at 60 ℃ to primarily inhibit pollen germination and prevent the components of the pollen content from changing; then sieving the mixture by a 200-mesh sieve to remove impurities such as filaments, drug walls and the like;
(2) soaking the sieved pollen in 75% ethanol solution for 10min to thoroughly kill the pollen and keep the shape and structure of the pollen intact, and facilitating the subsequent micro-alkali treatment effect; centrifuging at 3000 rpm for 1min, and removing the supernatant;
(3) the pollen soaked in the ethanol solution in the step (2) adopts the concentration of 2.0 multiplied by 10－3Treating with mol/L sodium carbonate solution for 10 min; centrifuging at 3000 rpm for 1min, and removing the supernatant;
(4) adding the pollen treated by the slightly alkaline solution in the step (3) into distilled water for rinsing, centrifuging for 1min at 3000 revolutions, discarding the supernatant, and rinsing repeatedly for one time; and then drying at 60 ℃ to obtain the pollen nano porous microspheres.
Example 2
(1) Selecting flowers in normal flowering phase of healthy peony groups to collect pollen, and specifically cutting stamen filaments by using tweezers or scissors, and pouring the cut stamen filaments into a collection bag for collection; drying the collected pollen at 60 ℃ to primarily inhibit pollen germination and prevent the components of the pollen content from changing; then sieving the mixture by a 200-mesh sieve to remove impurities such as filaments, drug walls and the like;
(2) soaking the sieved pollen in 75% ethanol solution for 10min to thoroughly kill the pollen and keep the shape and structure of the pollen intact, and facilitating the subsequent micro-alkali treatment effect; centrifuging at 3000 rpm for 1min, and removing the supernatant;
(3) the pollen soaked in the ethanol solution in the step (2) adopts the concentration of 3.0 multiplied by 10－3Treating with mol/L sodium carbonate solution for 10 min; centrifuging at 3000 rpm for 1min, and removing the supernatant;
(4) adding the pollen treated by the slightly alkaline solution in the step (3) into distilled water for rinsing, centrifuging for 1min at 3000 revolutions, discarding the supernatant, and rinsing repeatedly for one time; and then drying at 60 ℃ to obtain the pollen nano porous microspheres.
Example 3
(1) Selecting flowers in normal flowering phase of healthy peony groups to collect pollen, and specifically cutting stamen filaments by using tweezers or scissors, and pouring the cut stamen filaments into a collection bag for collection; drying the collected pollen at 60 ℃ to primarily inhibit pollen germination and prevent the components of the pollen content from changing; then sieving the mixture by a 200-mesh sieve to remove impurities such as filaments, drug walls and the like;
(2) soaking the sieved pollen in 75% ethanol solution for 10min to thoroughly kill the pollen and keep the shape and structure of the pollen intact, and facilitating the subsequent micro-alkali treatment effect; centrifuging at 3000 rpm for 1min, and removing the supernatant;
(3) the pollen soaked in the ethanol solution in the step (2) adopts the concentration of 4.0 multiplied by 10－3Treating with mol/L sodium carbonate solution for 10 min; centrifuging at 3000 rpm for 1min, and removing the supernatant;
(4) adding the pollen treated by the slightly alkaline solution in the step (3) into distilled water for rinsing, centrifuging for 1min at 3000 revolutions, discarding the supernatant, and rinsing repeatedly for one time; and then drying at 60 ℃ to obtain the pollen nano porous microspheres.
Example 4
(1) Selecting flowers in normal flowering phase of healthy peony groups to collect pollen, and specifically cutting stamen filaments by using tweezers or scissors, and pouring the cut stamen filaments into a collection bag for collection; drying the collected pollen at 80 ℃ to primarily inhibit pollen germination and prevent the components of the pollen content from changing; then sieving the mixture by a 200-mesh sieve to remove impurities such as filaments, drug walls and the like;
(2) soaking the sieved pollen in 100% ethanol solution for 5min to thoroughly kill the pollen and keep the shape and structure of the pollen intact, and facilitating the subsequent micro-alkali treatment effect; centrifuging at 2500 rpm for 2min, and removing the supernatant;
(3) the pollen soaked in the ethanol solution in the step (2) adopts the concentration of 2.0 multiplied by 10－3Treating with mol/L sodium carbonate solution for 8 min; centrifuging at 2500 rpm for 2min, and removing the supernatant;
(4) adding the pollen treated by the slightly alkaline solution in the step (3) into distilled water for rinsing, centrifuging for 2min at 2500 rpm, discarding the supernatant, and rinsing repeatedly for one time; then drying at 120 ℃ to obtain the pollen nano porous microspheres.
Example 5
(1) Selecting flowers in normal flowering phase of healthy peony groups to collect pollen, and specifically cutting stamen filaments by using tweezers or scissors, and pouring the cut stamen filaments into a collection bag for collection; drying the collected pollen at 120 ℃ to primarily inhibit pollen germination and prevent the components of the pollen content from changing; then sieving the mixture by a 200-mesh sieve to remove impurities such as filaments, drug walls and the like;
(2) soaking the sieved pollen in 95% ethanol solution for 15min to thoroughly kill the pollen and keep the shape and structure of the pollen intact, and facilitating the subsequent micro-alkali treatment effect; centrifuging at 4000 rpm for 0.5min, and removing the supernatant;
(3) the pollen soaked in the ethanol solution in the step (2) adopts the concentration of 2.0 multiplied by 10－3Treating with mol/L sodium carbonate solution for 15 min; centrifuging at 4000 rpm for 0.5min, and removing the supernatant;
(4) adding the pollen treated by the slightly alkaline solution in the step (3) into distilled water for rinsing, centrifuging for 0.5min at 4000 revolutions, removing supernatant, and rinsing repeatedly twice; and then drying at 80 ℃ to obtain the pollen nano porous microspheres.
Comparative example 1
(1) Selecting flowers in normal flowering phase of healthy peony groups to collect pollen, and specifically cutting stamen filaments by using tweezers or scissors, and pouring the cut stamen filaments into a collection bag for collection; drying the collected pollen at 60 ℃ to primarily inhibit pollen germination and prevent the components of the pollen content from changing; then sieving the mixture by a 200-mesh sieve to remove impurities such as filaments, drug walls and the like;
(2) soaking the sieved pollen in 75% ethanol solution for 10min to thoroughly kill the pollen and keep the shape and structure of the pollen intact, and facilitating the subsequent micro-alkali treatment effect; centrifuging at 3000 rpm for 1min, and removing the supernatant;
(3) the pollen soaked in the ethanol solution in the step (2) adopts the concentration of 1.0 multiplied by 10－3Treating with mol/L sodium carbonate solution for 10 min; centrifuging at 3000 rpm for 1min, and removing the supernatant;
(4) adding the pollen treated by the slightly alkaline solution in the step (3) into distilled water for rinsing, centrifuging for 1min at 3000 revolutions, discarding the supernatant, and rinsing repeatedly for one time; and then drying at 60 ℃ to obtain the pollen nano porous microspheres.
Comparative example 2
(1) Selecting flowers in normal flowering phase of healthy peony groups to collect pollen, and specifically cutting stamen filaments by using tweezers or scissors, and pouring the cut stamen filaments into a collection bag for collection; drying the collected pollen at 60 ℃ to primarily inhibit pollen germination and prevent the components of the pollen content from changing; then sieving the mixture by a 200-mesh sieve to remove impurities such as filaments, drug walls and the like;
(2) soaking the sieved pollen in 75% ethanol solution for 10min to thoroughly kill the pollen and keep the shape and structure of the pollen intact, and facilitating the subsequent micro-alkali treatment effect; centrifuging at 3000 rpm for 1min, and removing the supernatant;
(3) the pollen soaked in the ethanol solution in the step (2) adopts the concentration of 5.0 multiplied by 10－3Treating with mol/L sodium carbonate solution for 10 min; centrifuging at 3000 rpm for 1min, and removing the supernatant;
(4) adding the pollen treated by the slightly alkaline solution in the step (3) into distilled water for rinsing, centrifuging for 1min at 3000 revolutions, discarding the supernatant, and rinsing repeatedly for one time; and then drying at 60 ℃ to obtain the pollen nano porous microspheres.
Comparative example 3
(1) Selecting flowers in normal flowering phase of healthy peony groups to collect pollen, and specifically cutting stamen filaments by using tweezers or scissors, and pouring the cut stamen filaments into a collection bag for collection; drying the collected pollen at 60 ℃ to primarily inhibit pollen germination and prevent the components of the pollen content from changing; then sieving the mixture by a 200-mesh sieve to remove impurities such as filaments, drug walls and the like;
(2) soaking the sieved pollen in 75% ethanol solution for 10min to thoroughly kill the pollen and keep the shape and structure of the pollen intact, and facilitating the subsequent micro-alkali treatment effect; centrifuging at 3000 rpm for 1min, and removing the supernatant;
(3) soaking the ethanol solution in the step (2)The pollen has a concentration of 10.0 × 10－3Treating with mol/L sodium carbonate solution for 10 min; centrifuging at 3000 rpm for 1min, and removing the supernatant;
(4) adding the pollen treated by the slightly alkaline solution in the step (3) into distilled water for rinsing, centrifuging for 1min at 3000 revolutions, discarding the supernatant, and rinsing repeatedly for one time; and then drying at 60 ℃ to obtain the pollen nano porous microspheres.
Effects of the embodiment
(1) Scanning electron microscope experiment
The untreated pollen, the pollen nano porous microspheres prepared in the examples 1 to 3 and the comparative examples 1 to 3 were observed by a scanning electron microscope, and the specific method was as follows: randomly cutting the glass slide by using a single-sided safety blade, spraying gold on an SBC-12 small particle sputtering instrument (Beijing Zhongke instrument), and observing and photographing on a Phenom Pure desktop scanning electron microscope (femto). After the contrast pollen is dried, no other treatment is carried out, and only the slide glass is randomly cut by a single-sided guard blade, sprayed with gold and observed by a scanning electron microscope to take a picture.
FIG. 1 is a scanning electron micrograph of pollen which was directly cut without any treatment, and it can be seen that the pollen was full of contents.
FIG. 2 is the SEM image of the cut pollen nano-porous microspheres prepared in example 1, and it can be seen that the concentration is 2.0 × 10－3The content in the pollen can be completely dissolved out by treating with the mol/L sodium carbonate solution, and the pollen can be kept not to collapse, so that the yield is over 80 percent.
FIG. 3 is the scanning electron microscope image of the cut pollen nano porous microsphere prepared in example 2, which shows that the adopted concentration is 3.0X 10－3The content in the pollen can be completely dissolved out by treating with a sodium carbonate solution of mol/L, but the pollen collapses.
FIG. 4 is the SEM image of the cut pollen nano-porous microspheres obtained in example 3, and it can be seen that the concentration is 4.0 × 10－3The degradation degree of the pollen wall is deepened and the pollen collapse ratio is further increased by processing the sodium carbonate solution of mol/L.
FIG. 5 is a comparative example1, the obtained pollen nano porous microspheres are cut and then are subjected to a scanning electron microscope, and the scanning electron microscope can be seen from the figure, wherein the concentration of the pollen nano porous microspheres is 1.0 multiplied by 10－3When pollen is treated with a sodium carbonate solution of mol/L, the contents in the pollen cannot be completely dissolved out.
FIG. 6 is a scanning electron micrograph of the cut pollen nanoporous microspheres obtained in comparative example 2, from which it can be seen that the pollen nanoporous microspheres with a concentration of 5.0 × 10 is used－3After the treatment of the sodium carbonate solution of mol/L, the pollen wall is degraded excessively, and a large amount of collapse is seen.
FIG. 7 is a scanning electron micrograph of the cut pollen nanoporous microspheres obtained in comparative example 3, from which it can be seen that the concentration of 10.0 × 10 is used－3The pollen wall is degraded excessively and almost completely collapses when being treated by the sodium carbonate solution of mol/L.
(2) Toxicity test in mice
10 mice (purchased from the center of experimental animals in Henan province, weighing 30g and age 50 days) were taken, and the pollen nanoporous microspheres prepared in example 1 were diluted to a concentration of 50 ten thousand particles/ml by tail vein injection, and then injected 1 time per hour, 0.1ml each time, 4 times continuously for a total of 0.4ml (20 ten thousand particles of pollen).
The experimental result shows that: mice were able to tolerate during and after injection without any apparent symptoms. The expected cardiovascular and cerebrovascular blockage did not occur at all.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (10)
1. A preparation method of pollen nano porous microspheres is characterized by comprising the following steps:
(1) pretreating pollen to primarily inhibit pollen germination, and then sieving;
(2) soaking the sieved pollen in 75-100% by volume of ethanol solution for 5-15 min; centrifuging and discarding the supernatant;
(3) treating the pollen soaked in the ethanol solution in the step (2) for 8-15 min by using a slightly alkaline solution; centrifuging and discarding the supernatant;
(4) rinsing the pollen treated by the slightly alkaline solution in the step (3); and then drying to obtain the pollen nano porous microspheres.
2. The method for preparing the pollen nano porous microspheres of claim 1, wherein the method comprises the following steps:
the pretreatment mode in the step (1) is at least one of drying, microwave treatment and ray treatment.
3. The method for preparing the pollen nano porous microspheres of claim 1, wherein the method comprises the following steps:
the drying temperature is 60-120 ℃.
4. The method for preparing the pollen nano porous microspheres of claim 1, wherein the method comprises the following steps:
the sieving mesh number in the step (1) is 200 meshes.
5. The method for preparing the pollen nano porous microspheres of claim 1, wherein the method comprises the following steps:
the micro-alkali solution in the step (3) has the concentration of 2.0 multiplied by 10－3mol/L ~5.0×10－3mol/L sodium carbonate solution or pH equivalent concentration of 2.0X 10－3mol/L ~5.0×10－3Other alkali solution of sodium carbonate solution in mol/L.
6. The method for preparing the pollen nano porous microspheres according to claim 5, wherein the method comprises the following steps:
the micro-alkali solution in the step (3) has the concentration of 2.0 multiplied by 10－3mol/L ~4.0×10－3mol/L sodium carbonate solution or pH equivalent concentration of 2.0X 10－3mol/L ~4.0×10－3Others in mol/L sodium carbonate solutionAn alkaline solution.
7. The method for preparing the pollen nano porous microspheres of claim 1, wherein the method comprises the following steps:
the rinsing mode in the step (4) is as follows: and (4) mixing the pollen treated by the slightly alkaline solution in the step (3) with water, rinsing, centrifuging and discarding the supernatant.
8. The method for preparing the pollen nano porous microspheres of claim 1, wherein the method comprises the following steps:
and (4) drying in a natural drying, drying or freeze-drying manner.
9. A pollen nano porous microsphere is characterized by being prepared by the preparation method of any one of claims 1 to 8.
10. The pollen nano porous microsphere of claim 9, which is applied to the fields of biomedicine and cosmetics.
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| CN115232790A (en) * | 2022-07-29 | 2022-10-25 | 康元医疗科技(大连)有限公司 | Pollen particle, preparation method, biological functional material using same and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104941544A (en) * | 2015-05-29 | 2015-09-30 | 蔡文 | Sporopollen microcapsule, and preparation method and application thereof |
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| CN104941544A (en) * | 2015-05-29 | 2015-09-30 | 蔡文 | Sporopollen microcapsule, and preparation method and application thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115232790A (en) * | 2022-07-29 | 2022-10-25 | 康元医疗科技(大连)有限公司 | Pollen particle, preparation method, biological functional material using same and application |
| CN115232790B (en) * | 2022-07-29 | 2023-09-12 | 康元医疗科技(大连)有限公司 | Pollen particles, preparation method, biological functional material using the same and application thereof |
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