CN111065278A - Shrimp feed additive and preparation method thereof - Google Patents
Shrimp feed additive and preparation method thereof Download PDFInfo
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- CN111065278A CN111065278A CN201880030425.3A CN201880030425A CN111065278A CN 111065278 A CN111065278 A CN 111065278A CN 201880030425 A CN201880030425 A CN 201880030425A CN 111065278 A CN111065278 A CN 111065278A
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- mixture
- soybeans
- acidified
- bacillus subtilis
- distilled water
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- 239000003674 animal food additive Substances 0.000 title claims abstract description 7
- 241000143060 Americamysis bahia Species 0.000 title description 5
- 238000002360 preparation method Methods 0.000 title description 2
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 60
- 244000068988 Glycine max Species 0.000 claims abstract description 50
- 239000000203 mixture Substances 0.000 claims abstract description 46
- 241000238557 Decapoda Species 0.000 claims abstract description 30
- 229930091371 Fructose Natural products 0.000 claims abstract description 21
- 239000005715 Fructose Substances 0.000 claims abstract description 21
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims abstract description 21
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 18
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 18
- 239000000463 material Substances 0.000 claims abstract description 14
- 238000004519 manufacturing process Methods 0.000 claims abstract description 7
- 238000000855 fermentation Methods 0.000 claims abstract description 6
- 230000004151 fermentation Effects 0.000 claims abstract description 6
- 238000002156 mixing Methods 0.000 claims abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 239000012153 distilled water Substances 0.000 claims description 20
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 9
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 9
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 9
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 5
- 238000002203 pretreatment Methods 0.000 claims description 5
- 102000002812 Heat-Shock Proteins Human genes 0.000 claims description 4
- 108010004889 Heat-Shock Proteins Proteins 0.000 claims description 4
- 241000208829 Sambucus Species 0.000 claims description 3
- 235000018735 Sambucus canadensis Nutrition 0.000 claims description 3
- 235000007123 blue elder Nutrition 0.000 claims description 3
- 230000009089 cytolysis Effects 0.000 claims description 3
- 235000007124 elderberry Nutrition 0.000 claims description 3
- 235000008995 european elder Nutrition 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 2
- 239000008103 glucose Substances 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims description 2
- 238000007599 discharging Methods 0.000 claims 1
- 150000003628 tricarboxylic acids Chemical class 0.000 claims 1
- 230000001418 larval effect Effects 0.000 description 5
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 102000002689 Toll-like receptor Human genes 0.000 description 2
- 108020000411 Toll-like receptor Proteins 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- 231100000517 death Toxicity 0.000 description 2
- 239000006052 feed supplement Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241001126836 Enterocytozoon Species 0.000 description 1
- 241001385463 Hepatica <moth> Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 229920006328 Styrofoam Polymers 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- 239000003570 air Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000004463 hay Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000008177 pharmaceutical agent Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920006327 polystyrene foam Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000008261 styrofoam Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Insects & Arthropods (AREA)
- Marine Sciences & Fisheries (AREA)
- Birds (AREA)
- Sustainable Development (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
A method for producing a shrimp feed additive, comprising a first step of preparing a pre-treated mixture of germinated soybeans; a second step of preparing activated Bacillus subtilis; a third step of adding activated Bacillus subtilis to the pre-treated mixture of germinated soybeans to produce a first mixture; a fourth step of taking out the active soybean material from the first mixture; a fifth process of mixing the activated soybean material and the acidified fructose solution into sterilized shrimp shells to obtain a second mixture, and fermenting the second mixture to produce a fermented mixture; and a sixth step of taking out the top layer of the fermentation mixture.
Description
Technical Field
The invention relates to a shrimp feed and a production method thereof. More particularly, the present invention relates to a shrimp feed additive and a method for producing the same, which can improve the survival rate of young shrimp (post larval) in the shrimp larvae period.
Background
The following discussion of the background to the invention is intended to enhance an understanding of the invention. However, it should be understood that this discussion is not a statement or admission that any of the material referred to was published, known or part of the common general knowledge of a person skilled in the art in any jurisdiction as of the filing date of the application.
The shrimp farming industry is an industry that costs millions of dollars. Land farmers are subject to a large number of deaths of shrimp larvae from the flea larval stage to the larval stage up to 30-60 days of culture (DOC 30-60) under the influence of new diseases. Others are afflicted with diseases such as shrimp Enterocytozoon Hepatica (EHP) which do not kill shrimp completely, but rather severely retard or retard shrimp growth and increase Feed Conversion Ratio (FCR). Therefore, it takes longer for EHP infected shrimps to reach a minimum size of 10-12g at DOC100, while farmers should obtain an average of 15-18g of shrimps at DOC 100.
To increase the survival rate and growth rate of shrimp larvae cultivated in the shrimp larvae, farmers currently grow shrimp by feeding the larvae with several antibiotic, chemical or pharmaceutical agents during the shrimp larvae to overcome the massive mortality of the larvae, which are usually toxic and carcinogenic to the human body when the shrimp are consumed.
Bacillus subtilis (Bacillus subtilis) is an aerobic bacterium that forms spores, and has no known toxicity. It is produced in nature, including hay, soil, sewage and air, and is capable of enzymatically achieving curd, saccharifying starch and degrading lipids. Bacillus subtilis is also widely used in industrial fields. It grows optimally at a pH of 7-8.5, with a temperature set at 37-40 ℃.
The present invention aims at least partly to overcome some of the above disadvantages and to provide an alternative solution which improves the survival rate and growth rate of farmed shrimp and reduces the incidence of diseases originating from the hatchery.
Disclosure of Invention
Throughout this document, unless stated to the contrary, the terms "comprising," consisting of, "and the like are to be construed as non-exhaustive or, in other words, mean" including but not limited to.
According to a first aspect of the present invention, there is provided a method of producing a shrimp feed supplement comprising:
i. a first step of preparing a pretreatment mixture of germinated soybeans;
a second step of preparing activated Bacillus subtilis;
a third process step of adding activated bacillus subtilis to the pre-treated mixture of germinated soybeans to produce a first mixture;
a fourth step of removing the activated soybean material from the first mixture;
v. a fifth process step of mixing the activated soy material and the acidified fructose solution into sterilized shrimp shells to obtain a second mixture, fermenting the second mixture to produce a fermented mixture; and
a sixth procedure, the top layer of the fermentation mixture is taken out.
Preferably the pre-treated mixture of germinated soybeans is prepared by the following method:
i. repeatedly washing the soybeans with distilled water, and pouring the distilled water into a container;
adding preheated distilled water to the soybeans to produce a soybean mixture, incubating the soybean mixture to stimulate production of enzymes and heat shock proteins in the soybeans; and
removing distilled water from the soybean mixture to obtain expanded soybeans, and adding citric acid to the expanded soybeans.
Activated Bacillus subtilis is preferably prepared by modifying a Bacillus subtilis culture to induce hyperstimulation of the tricarboxylase enzyme.
Over-stimulation of the tricarboxylases is preferably induced by acidified sugar molecules.
Preferably the acidified sugar molecule is an acidified glucose molecule or an acidified fructose molecule.
The acidified fructose solution is preferably prepared by adding acetic acid and a proprietary extract comprising fermented elderberry to fructose.
Bacillus subtilis cultures are preferably prepared using Bacillus SZ-2010(Bacillus SZ-2010) with a Luria-Betani (Luria Bertani) or lysis Broth (Lysogeniy Broth) (LB) medium.
According to a second aspect of the invention there is provided a shrimp feed additive produced according to the method of the first aspect of the invention.
Detailed Description
Specific embodiments of the present invention will now be described in detail. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the present invention. Other embodiments may be utilized and structural and logical changes may be made without departing from the scope of the present invention. The various embodiments are not necessarily mutually exclusive, as some embodiments may be combined with one or more other embodiments to form new embodiments. In addition, unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
In the description, the term "comprising" is to be understood as having a similar broad meaning as the term "comprising" and is to be understood as implying that an integer or step or group of integers or steps is included, but not excluding any other integer or step or group of integers or steps. This definition also applies to variants of the term "comprising", such as "having" and "with".
According to a first embodiment of the present invention, there is a method of producing a shrimp feed supplement comprising the following steps.
First step of
The method comprises a first step of preparing a ready-to-use pre-treatment mixture of germinated soybeans, as follows:
(a) 3 kilograms (kg) of soybeans were washed with 5 liters (L) of distilled water at 15 degrees Celsius (. degree.C.). This cleaning procedure was repeated at least 3 times. The soybeans were then placed in a 10L container, added with 15 deg.C distilled water and washed by hand rubbing. The washed soybeans were then discharged into a metal sieve and transferred to another 10L container, where the soybeans were soaked in distilled water at 15 ℃ for 12(+/-1) hours, and then the distilled water was completely discharged. The above parameters are preferably increased or decreased in various ratios. Thus, if 6 kg of soybeans are used, 10 liters of distilled water should be used accordingly to ensure that the soybeans absorb sufficient distilled water. It was observed that a 10L container would maintain the soy and distilled water mixture approximately thermostatically for 12(+/-1) hours.
(b) 6L of sterilized distilled water was further heated to 30 ℃ and added to the washed and discharged soybeans as described in (a) above. The mixture is then incubated for 4-6 hours in a plastic container, preferably made of polystyrene foam (styrofoam), to keep the soy and distilled water mixture near constant temperature. It was observed that a temperature of 30 ℃ stimulates the production of enzymes in soybeans, resulting in the production of superoxide di-methane (soyabean) by soybeans.
(c) Then the water from the mixture of soybeans and distilled water was drained and another 6L of sterilized distilled water at 42 ℃ was added to the washed and drained soybeans and the resulting mixture was incubated for 24 hours, after which the distilled water was completely drained, leaving the swollen soybeans. Low molecular weight Heat Shock Proteins (HSPs) are activated and produced by soybeans.
(d) The expanded soybeans are then transferred to a 5L container, preferably a non-metallic container such as a glass bottle. 2L of 0.1% citric acid solution was prepared and added to the swollen soybeans. The entire mixture was held at 37(+/-2) ° c for 24 hours or until radicle or root length of the swollen soybeans was observed to be between 2-3 millimeters (mm).
The second step is that:
the Bacillus subtilis culture is next modified to induce over-stimulation of the tricarboxylase enzyme, which produces activated Bacillus subtilis. Over-stimulation of the tricarboxylases is induced by the acidified sugar molecule. In this embodiment of the invention, it is preferred to use acidified fructose molecules. An acidified fructose solution is prepared by adding 5mL of 1% w/v acetic acid to 10g of fructose in a closed vessel, such as a container, to prevent crystallization of the acidified fructose solution. The acidified fructose solution is allowed to react for 1 to 6 hours, preferably 1 hour, at 60 ℃ before use. The acidified fructose solution must be allowed to react for no more than 6 hours to prevent the formation of foreign metabolites in the solution. After 1 hour, 1-5 mL (mL) of proprietary extract (ProprietaryExtract) was added to the acidified fructose solution. Proprietary extracts comprise fermented elderberry using bacillus SZ-0097. Preferably 1mL of proprietary extract is added to the acidified fructose solution. Acidified fructose solutions were prepared in volumes exceeding 100 mL.
Bacillus subtilis cultures were prepared by co-culturing using proprietary single colony Bacillus SZ-2010, or variants thereof, with Luria-Betani (Luria Bertani) or lysis Broth (Lysogeniy Broth) (LB) medium at room temperature for 18 hours. 10mL of Bacillus SZ-2010 was aliquoted to 900mL of 1/10LB medium concentration and incubated at 37 ℃ for 3 hours.
Then 90mL of acidified fructose solution was added to proprietary bacillus SZ-2010 to make 1L and incubated at 43 ℃ for at least 8 hours to produce activated bacillus SZ-2010.
The third step:
500mL of activated Bacillus SZ-2010 was then added to 500g of the ready-to-use germinated soybean pretreatment mixture produced in step 1 to form a mixture and allowed to ferment at room temperature for 21-28 days.
The fourth step:
after 21-28 days, the liquid top layer of the mixture prepared in step 3 (activated soy material) was removed. It is preferred to remove 60-150mL of activated soy material, since it is observed that volumes in excess of 150mL will contain precipitates produced by fermentation of the ready-to-use soybeans and the presence of such deposits will reduce the efficacy of the activated soy material. More preferably, 100mL of the activated soy material is withdrawn. The removed activated soybean material is preserved at 4 deg.C for no more than 365 days. The activated soy material must not be stored at temperatures below 4 ℃ to prevent freezing of the liquid and thereby affect its efficacy.
The fifth step:
100mL of activated soy material prepared as described in step 2 above and 100mL of acidified fructose solution were added to 1Kg sterilized shrimp shells. The mixture was allowed to ferment for 365 days.
After about 6 months of fermentation, an additional 100mL of acidified fructose solution was added. The mixture is now allowed to ferment for a further 6 months.
And a sixth step:
after 1 year of fermentation, the top liquefied layer of the resulting fermented mixture (also called Prime Up Toll-Like Receptor (TLR)) is removed and can be used as a shrimp feed additive to improve the survival rate of young shrimp in the hatchery during the larval stage.
The invention has the following advantages:
1. the present invention reduces disease and mortality in shrimp cultures in the hatchery state prior to growth.
2. Shrimp fed with the feed produced according to the invention have been shown to be resistant to protozoan attachment during the flea larval stage, for which no treatment has previously been known. Studies have shown that 50-90% of shrimp larvae will be subject to death within 2 days once protozoan attachment occurs.
3. The shrimp feed is prepared from natural components which are nontoxic or harmless to human bodies.
It will be appreciated by persons skilled in the art that the above invention is not limited to the described embodiments. In particular, the following modifications and improvements may be made without departing from the scope of the invention:
different natural bacterial or fungal strains may be used instead of the pre-treatment mixture of germinated soybeans.
It will be understood that the embodiments of the present invention described above are provided by way of example only and that further modifications and improvements thereto will be apparent to those skilled in the relevant art and fall within the scope of the invention. It is also to be understood that features from one or more of the described embodiments may be combined to form further embodiments.
Claims (8)
1. A method of producing a shrimp feed additive, comprising:
a first step of preparing a pre-treatment mixture of germinated soybeans;
a second step of preparing activated Bacillus subtilis;
a third step of adding the activated bacillus subtilis to the pre-treatment mixture of germinated soybeans to produce a first mixture;
a fourth step of taking out the active soybean material from the first mixture;
a fifth step of mixing the activated soy material and the acidified fructose solution into sterilized shrimp shells to obtain a second mixture, fermenting the second mixture to produce a fermented mixture; and
a sixth step of taking out the top layer of the fermentation mixture.
2. A method according to claim 1, characterized in that:
the pre-treatment mixture of germinated soybeans was prepared by the following method:
repeatedly washing the soybeans with distilled water, and discharging the distilled water into a container;
adding preheated distilled water to the soybeans to produce a soybean mixture, incubating the soybean mixture to stimulate production of enzymes and heat shock proteins in the soybeans; and
distilled water is drained from the soybean mixture to obtain expanded soybeans, and citric acid is added to the expanded soybeans.
3. A method according to claim 1, characterized in that:
the activated Bacillus subtilis is prepared by modifying a Bacillus subtilis culture to induce hyperstimulation of the tricarboxylase.
4. A method according to claim 3, characterized in that:
over-stimulation of the tricarboxylic acid enzyme is induced by the acidified sugar molecule.
5. The method according to claim 4, characterized in that:
the acidified sugar molecule is an acidified glucose molecule or an acidified fructose molecule.
6. A method according to claim 1, characterized in that:
the acidified fructose solution is prepared by adding acetic acid and a proprietary extract comprising fermented elderberry to fructose.
7. The method of claim 3, wherein:
the bacillus subtilis culture was prepared using bacillus SZ-2010 with luria-beta or lysis broth medium.
8. A shrimp feed, characterized in that:
produced according to the process of claim 1.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/SG2018/050351 WO2020018013A1 (en) | 2018-07-16 | 2018-07-16 | Shrimp feed additive and method for producing the same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111065278A true CN111065278A (en) | 2020-04-24 |
Family
ID=69165211
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201880030425.3A Pending CN111065278A (en) | 2018-07-16 | 2018-07-16 | Shrimp feed additive and preparation method thereof |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN111065278A (en) |
| MY (1) | MY202515A (en) |
| WO (1) | WO2020018013A1 (en) |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3154365B1 (en) * | 2014-05-29 | 2020-12-16 | Ohio Soybean Council | Mitigation of anti-nutritional substances in plant meal |
| CN107549508A (en) * | 2017-08-15 | 2018-01-09 | 兰溪市酉泽饲料技术服务有限公司 | Promote the preparation method of the feed of prawn husking |
| CN107736501A (en) * | 2017-10-24 | 2018-02-27 | 安徽省家牧动物营养科技有限公司 | A kind of processing method of prawn feed |
| CN107821873A (en) * | 2017-12-04 | 2018-03-23 | 李六秀 | The preparation method of the feed of gut of shrimp function can be improved |
| CN107927449A (en) * | 2017-12-04 | 2018-04-20 | 李六秀 | The preparation method of microbial fermentation prawn feed |
-
2018
- 2018-07-16 CN CN201880030425.3A patent/CN111065278A/en active Pending
- 2018-07-16 MY MYPI2019005253A patent/MY202515A/en unknown
- 2018-07-16 WO PCT/SG2018/050351 patent/WO2020018013A1/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| WO2020018013A1 (en) | 2020-01-23 |
| MY202515A (en) | 2024-05-02 |
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