CN111040962A - New strain marine bacterial strain XAAS-72 and application thereof in plant antibiosis and growth promotion - Google Patents
New strain marine bacterial strain XAAS-72 and application thereof in plant antibiosis and growth promotion Download PDFInfo
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Abstract
The invention discloses a new strain in marine bacteriaPontibacterspXAAS-72 and its use in plant antimicrobial growth promotion. Screening a marine bacterial strain from a plurality of batches of bacterial strains separated from salicornia europaea rhizosphere soil in Leptospermum scoparium area of XinjiangPontibacterspXAAS-72 CGMCC No.16594, the separated strain has the characteristics of antagonizing various bacterial pathogenic bacteria, salt tolerance, growth promotion, phosphate dissolving, indoleacetic acid secretion and ACC deaminase production, and the like, and is scientifically identified by strain classification to belong to a new strain in marine bacteriaPontibacter spThe strain liquidThe fermentation liquid preparation can be applied to promoting plant growth and preventing and treating plant diseases, has obvious and stable technical effect, and has wide application value in the technical field of microbial strain application.
Description
Technical Field
The invention relates to the technical field of microbial strains and application thereof, in particular to the technical field of marine bacterial strains and application thereof in plant antibiosis and growth promotion.
Background
The plant is easy to be affected by bacteria and other environments during normal growth, and under the condition of proper environment, the bacteria can interfere and destroy the metabolism and normal development of the plant through transmission and reproduction, so that the plant body is induced to attack, the plant growth is finally weakened, the yield is reduced, and the quality is reduced. In particular, in the continuous cropping planted land, bacterial diseases are commonly caused due to the imbalance of flora in the soil, such as common crop diseases including wheat and rice leaf spot, potato soft rot, cucumber bacterial angular leaf spot in vegetables, eggplant bacterial wilt, soft rot and the like, which are all caused by pathogenic bacteria. Common pathogenic bacteria are related to various bacteria of the genera Xanthomonas, Pseudomonas, Erwinia, Agrobacterium, Streptomyces, and the like.
At present, the prevention and treatment of bacterial diseases are mainly carried out by means of measures such as breeding of disease-resistant varieties and strengthening of field management, and the strengthening of field management is a main means for prevention and treatment of agricultural bacterial diseases, and chemical prevention and treatment is one of important methods. Along with the enhancement of awareness of people on food safety and environmental protection, the use of chemical pesticides causes pollution and damage to the environment, agricultural production and food safety, and the contradiction is increasingly prominent. In recent years, with the strategic arrangement of 'two-reduction-one-increase' and the rise of green organic agriculture, the application of biological control technology to replace or partially replace pesticides is actively explored, and the biological control technology becomes one of the important points of agricultural production research.
The biological control technology is a technology and a method for effectively controlling crop diseases by utilizing various beneficial organisms and active substances and secretions generated by the beneficial organisms, and particularly, the microbial control is one of important methods for biological control. The method effectively improves the soil, accelerates the healthy growth of plants, has the basic characteristics of high efficiency, no pollution, no harm, no toxicity and the like, meets the requirements of people on green foods, and can ensure the sustainable development of agriculture. Under such a background, a class of green and efficient microorganism prevention and control measures represented by plant growth-promoting bacteria is increasingly paid attention to. The plant growth-promoting bacteria have important effects on inhibiting the occurrence of soil-borne diseases and improving and maintaining the ecological quality of soil and have wide development prospects. The plant growth-promoting bacteria are utilized to develop disease-resistant biological fertilizer and biological pesticide, so that the infection of pathogenic bacteria can be effectively inhibited, the influence of the plant on resisting adversity stress is relieved, and the effects of preventing diseases, promoting growth and increasing yield are achieved.
Although our country has many researches on promoting microbial resources, in the microbial control industry, strains used for many years are bacillus, paenibacillus and the like, and the problems of single strain, unstable application effect and the like exist, so that a new strain with high activity and stable function needs to be screened to promote the vigorous development of the microbial agent bacterial fertilizer industry.
Disclosure of Invention
Aiming at the current situation that a novel strain Pontibacter sp.XAAS-72 in marine bacteria is not obtained by separating and screening salicornia rhizosphere soil in the region of Marina in Xinjiang, the invention provides a novel strain Pontibacter sp.XAAS-72 in marine bacteria and application thereof in plant antibacterial growth promotion. The invention screens Pontibacter sp.XAAS-72 CGMCC No.16594 in a marine bacterial strain from a plurality of batches of separated bacterial strains in salicornia europaea rhizosphere soil in Xinjiang Marina, utilizes the characteristics that the separated bacterial strain has the capabilities of antagonizing a plurality of bacterial pathogenic bacteria, resisting salt, promoting growth, dissolving phosphorus, secreting indoleacetic acid, producing ACC deaminase and the like, and can know that the new bacterial strain Pontibacter p belongs to the marine bacterial strain through scientific identification of strain classification.
The invention adopts the main technical scheme that:
the invention specifically provides a Pontibacter sp.XAAS-72 CGMCC No.16594 in marine bacteria. A new bacterial strain Pontibacter sp of marine genus with the serial number of XAAS-72 is screened and separated from salicornia rhizosphere soil in the region of Mantis in Xinjiang, the bacterial strain Pontibacter sp.XAAS-72 is known to be a new bacterial strain through the existing common bacterial strain identification means, and the separated bacterial strain has the characteristics of antagonizing various bacterial pathogenic bacteria, resisting salt, promoting growth, dissolving phosphorus, secreting indoleacetic acid, producing ACC deaminase and the like.
Specifically, according to the particularity of the geographical environment of the Leptospermum scoparium area in Xinjiang, the invention cultures and separates the microbial strains of the rhizosphere soil of Leptospermum scoparium in the Leptospermum scoparium area in Xinjiang, screens a batch of excellent strains, separates and screens a Pontibacers p with the number of XAAS-72 from the excellent strains, classifies and identifies the strains to belong to marine bacteria through microbiology, the strains can grow under the conditions of 4-50 ℃ and 0-8% of NaCl, and the optimal growth conditions are as follows: the growth condition is optimal at 20-30 deg.C, pH6.0-7.0 and 0-1% NaCl, and the culture medium is MB agar medium. The XAAS-72 strain is determined to be a member of the marine genus by referring to a common strain identification manual such as Bergey's systematic bacteriology identification manual (ninth edition) and the like, and morphological, physiological and biochemical tests are carried out on the XAAS-72 strain, but the XAAS-72 strain has the characteristics different from the common marine genus member strain and has the characteristic of bright new strain.
Further, the invention carries out gene sequencing on the strain with the number of XAAS-72, the sequence is shown in SEQ ID NO.1 provided after the sequence is attached, the obtained sequence is compared and analyzed through a common website, a phylogenetic tree is constructed, and the comparison and analysis show that the 16S rDNA sequence of the strain marine bacterial strain and the standard mode Pontibacter korlensis X14-1TAnd Pontibacter litorisidinis YKTF-7THas the highest homology, the sequence similarity is 97.6 percent and 97.0 percent, the homology with other standard model bacteria of the genus is lower than 97.0 percent, and the strain XAAS-72 and the Pontibacter korlensis X14-1 have clear technical characteristics of new strainsTThe genetic relationship of (2) recently, a phylogenetic tree was established by a Neighbor-Joining method using MEGA 5.0 software commonly used in the art, and the results of comparison analysis found that the strains XAAS-72 and Pontibacter korlensis X14-1TThe confidence value of the strain is 77, and the strain XAAS-72 is determined to be a new species of Pontibacerp in marine genera through the molecular level identification of series strains, has the characteristic of typicality of the new species, and is tentatively named as Pontibacerp in marine genera XAAS-72 from the taxonomic point of view.
Specifically, the strain XAAS-72 has been deposited with the International depositary organization for microorganisms under the Budapest treaty: china general microbiological culture Collection center (CGMCC). Address: west road No.1, north west of the republic of kyo, yang, institute of microbiology, academy of sciences of china, zip code: 100101. the preservation date is 2018, 10 months and 16 days, and the preservation number of the strain is CGMCC No. 16594. The product is temporarily named Pontibacter sp. XAAS-72 by microbiological identification.
The new strain Pontibacter sp.XAAS-72 grows well on 1/3mB culture medium containing 1% NaCl, and forms visible colonies after being cultured for 72 hours at 30 ℃, and the colonies are round, convex, glossy, smooth and rose-red; the thallus is characterized by short rod-shaped, gram-negative bacteria, good oxygen and motility; growth is carried out at 4-50 deg.C and 0-8% NaCl, and growth conditions are optimized at 20-30 deg.C, pH6.0-7.0 and 0-1% NaCl.
Furthermore, the invention provides the Pontibacter sp.XAAS-72 CGMCC No.16594 metabolic biocontrol application, has the capabilities of antagonizing various bacterial pathogenic bacteria, resisting salt, promoting growth, solubilizing phosphorus, secreting indoleacetic acid, producing ACC deaminase and the like, has obvious and stable technical effects, can be applied to promoting plant growth and preventing and treating plant diseases, and has wide application value.
By implementing the specific technical scheme provided by the invention, the following beneficial effects can be achieved by implementing the content of the invention:
(1) the Pontibacter sp.XAAS-72 CGMCC No.16594 in marine bacteria is a typical new strain, and the strain can grow under the conditions of 4-50 ℃ and 0-8% NaCl. The optimal growth conditions are as follows: the growth condition is optimal under the conditions of 20-30 ℃, pH6.0-7.0 and 0-1% NaCl, the separated strains have the capabilities of antagonizing various bacterial pathogenic bacteria, resisting salt, promoting growth, decomposing phosphorus, secreting indoleacetic acid, producing ACC deaminase and the like, can be applied to promoting plant growth and preventing and treating plant diseases, and the provided new strains have wide application value.
(2) The invention provides a novel strain which utilizes the typical characteristics of Pontibacter sp.XAAS-72 CGMCC No.16594 in marine bacteria, has certain antagonism to various pathogenic bacteria of plants, can convert insoluble phosphorus in soil into effective phosphorus which can be directly absorbed and utilized by the plants, further promotes the growth of the plants and relieves the growth inhibition caused by phosphorus deficiency; the synthetic IAA can directly promote the growth of crops, enhance the disease resistance of the crops and effectively resist the invasion of pathogenic bacteria to a certain extent; the ACC dehydrogenase activity is realized, the content of harmful ethylene can be reduced, the growth of plants is promoted, and the influence of a stress environment is relieved; the marine bacterial sp. XAAS-72 can promote the growth of crops and enhance the disease resistance of the crops, thereby playing an active role in preventing and treating plant diseases.
(3) The separated and screened Pontibacter sp.XAAS-72 CGMCC No.16594 of the marine bacteria is cultured and activated, and diluted with sterilized water to prepare the living bacteria with the total concentration of 1 × 107The germination rate of cotton seeds soaked by the CFU/mL microbial inoculum through Pontibacter p.XAAS-72 microbial inoculum in marine bacteria is averagely improved by 2.3%, and the plant weight and plant height of wheat are respectively and obviously increased by 46.3% and 45.1% after the wheat grows for 30 days compared with those of contrast treatment, so that the CFU/mL microbial inoculum is suitable for promoting the growth of crops and enhancing the growth of the crops for industrial application of the bacterial strainsThe disease resistance treatment provides possibility.
Drawings
FIG. 1 shows a phylogenetic tree of Pontibacter sp.XAAS-72 CGMCC No. 16594.
FIG. 2 is a graph showing the effect of Pontibacter sp.XAAS-72 CGMCC No.16594 on wheat germination rate.
FIG. 3 is a graph showing the effect of Pontibacter sp.XAAS-72 CGMCC No.16594 on the fresh weight of wheat.
FIG. 4 is a graph showing the effect of Pontibacter sp.XAAS-72 CGMCC No.16594 on the plant height of wheat.
Detailed Description
The present invention will be described below with reference to examples, but the present invention is not limited to the following examples. All raw and auxiliary materials selected for use in the present invention, as well as methods for culturing the selected bacterial species, are well known and used in the art, and all percentages referred to herein are by weight unless otherwise indicated.
The following basic culture media are adopted in the embodiment of the invention:
MB medium: 5.0g peptone, 1.0g yeast extract, 0.1g ferric citrate, 19.45g NaCl, 5.9g mgCl2，3.24g Na2SO4，1.8g CaCl2，0.55g KCl， 0.16g NaHCO3，0.08g KBr，34.0mgSrCl2，22.0mg H3BO3，4.0mg Na2SiO3·9H2O，2.4mg NaF，1.6mg NH4NO3，8.0mg Na2HPO4Agar 15g, distilled water 1000ml, pH 7.6.
2% NaCl TSA-containing medium: 15g tryptone, 5g Soy peptone, 20g NaCl, 15g agar, H2O1000ml，pH 7.3。
Phosphate solubilizing bacteria culture medium: glucose 10.0g, yeast powder 0.5g, (NH)4)2SO40.5g、NaCl 20g、MgSO4·7H2O 0.3g、MnSO40.03g、K2SO40.3g、FeSO4·7H2O 0.03g、Ca3(PO4)25.0g agar 15.0g, H2O1000mL, pH 7.0-7.5.
DF culture medium: KH (Perkin Elmer)2PO44g、Na2HPO46g、MgSO4·7H20.2g of O, 2g of glucose, 2g of sodium gluconate, 2g of citric acid, (NH)4)2SO42g, 0.1mL of each of the trace element solutions of the component one and the component two, and FeSO4·7H2O 0.1mL、H2O1L, the samples are added one by one, fully dissolved and autoclaved for 20min at 121 ℃. Solution of trace elements: the component one: h3BO310mg、MnSO4·H2O11.19mg、ZnSO4·7H2O 124.6mg、 CuSO4·5H2O 78.22mg、MoO310mg, dissolved in 100mL sterile distilled water. And (2) component two: 100mg of FeSO4·7H2O was dissolved in 100mL of sterile distilled water.
The first embodiment is as follows: separation, screening and identification of Pontibacter sp.XAAS-72 CGMCC No.16594
(I) separating and screening:
collecting saline corniculate rhizosphere soil samples in the region of Marina in Xinjiang, and separating and purifying microorganisms by a gradient dilution method. Weighing 10g of soil sample, putting the soil sample into 100mL of sterile water, adding sterilized glass beads, and shaking at 200rpm and 30 ℃ for 20min to fully disperse the sample. Diluting with standard gradient and sterile water to obtain 10-2-10-5A dilution of concentration. 100. mu.L of each dilution was applied to a plate containing 1/3MB of a solid medium by a conventional application method, and the plate was cultured in a 30 ℃ incubator. After the bacterial colonies grow out of the plate in 48 hours, picking single bacterial colonies on the plate for purification culture. The single colony after purification is transferred to a TSA culture medium slant containing 2% NaCl and stored for later use.
(II) classification and identification:
1. sequencing and analysis of Pontibacter sp.XAAS-72 CGMCC No.1659416S rDNA:
(1) extraction of PCR template DNA:
inoculating the purified strain into 1/3MB solid culture medium, shake culturing at 30 deg.C for 2 days, collecting thallus, and extracting total genome DNA with DNA extraction kit.
(2) PCR amplification
Specific primers are adopted:
(2) PCR amplification
Specific primers are adopted:
27F:5'-AGAGTTTGATCCTGGCTCAG-3'，
1492R:5'-GGTTACCTTGTTACGACTT-3'；
the total volume of the PCR reaction system is 25 mu L, and the PCR amplification condition is 94 ℃ for 5 min; 94 ℃ for 45s, 56 ℃ for 45s, 72 ℃ for 45s, 30 cycles; 10min at 72 ℃.
(3) Sequence determination
The PCR amplification product is subjected to electrophoresis detection and purification and then sequenced to obtain a 16S rDNA sequence with the length of 1445bp, the sequence is shown in SEQ ID NO 1 provided after the sequence is attached, the obtained sequence is subjected to comparison analysis through a common NCBI website, a phylogenetic tree is constructed, the phylogenetic tree of the strain is shown in figure 1, and the comparison analysis shows that the strain XAAS-72 and the genus standard model Pontibacter korlensis X14-1TAnd Pontibacter litorisidinis YKTF-7TThe homology is highest, the sequence similarity is respectively 97.6 percent and 97.0 percent, a phylogenetic tree is established by a Neighbor-Joining method by utilizing MEGA 5.0 software commonly adopted in the field, and the results are compared and analyzed to find that the strains XAAS-72 and Pontibackkorerlensis X14-1TClustering on one branch, with a confidence value of 77, and identifying the strain XAAS-72 as a new species Pontibacter sp in the marine genus through series strain molecular level identification, wherein the strain has the characteristic of typicality of the new species and is tentatively named as Pontibacter sp.
2. Physiological and biochemical assays
(1) The research result of the growth condition shows that the Pontibacter sp.XAAS-72 CGMCC No.16594 is a gram-negative bacterium, aerobic, motile and rod-shaped. The colonies grow on 1/3MB agar to be round, convex, glossy, smooth and rose-red when growing for 3 days; can grow on 1/3MB, R2A and LB. The cells can grow under the conditions of 4-50 ℃ and 0-8% NaCl; the growth conditions are optimal at 20-30 deg.C, pH6.0-7.0 and 0-1% NaCl.
(2) Oxidase, milk coagulation test, lactopeptization, starch, cellulose hydrolysis and nitrate reduction reactions were all negative. The catalase and the gelatin test are both positive.
(3) Dextrin, D-maltose, D-trehalose, D-cellobiose, gentiobiose, sucrose, D-turanose, stachyose, raffinose, α -D-lactose, melibiose, D-salicin, N-acetyl- β -D-mannosamine, N-acetyl-D-galactosamine, N-acetylneuraminic acid, α -D-glucose, D-mannose, D-fructose, D-galactose, D-fructose, inosine, D-sorbitol, D-mannitol, D-arabitol, inositol, D-glucose-6-phosphate, D-fructose-6-phosphate, D-aspartic acid, gelatin, aminoacetyl-L-proline, L-alanine, L-arginine, L-aspartic acid, L-glutamic acid, L-histamine, L-pyroglutamic acid, L-serine, D-galacturonic acid, L-galacturonic acid lactone, D-mucic acid, gluconic acid, hydrabamic acid, L-glutamic acid, lactic acid, L-glutamic acid, L-pyroglutamic acid, malic acid, L-galacturonic acid, D-alanine, D-alanine, D-glutamic acid, D.
(4) In the API 20NE test strips, the reaction was positive for esculin, gelatin, and for nitro-D-methyl galactose.
The results of 16S rDNA sequence analysis, phylogenetic analysis and microbiological characteristic analysis show that the Pontibacter sp.XAAS-72 CGMCC No.16594 provided by the invention is a new bacterial strain in marine bacteria, and is tentatively named as Pontibacter sp. The strain has been preserved in China general microbiological culture Collection center (CGMCC) in 2018, 10 months and 16 days, and the address is as follows: the preservation number of the microorganism culture is CGMCC No.16594 in the great Tunnu of the Hongyang district of Beijing, China academy of sciences.
Example two: determination of antagonistic and growth promoting functions of Pontibacter sp.XAAS-72 CGMCC No.16594
(1) The method for measuring the antagonistic function comprises the following steps: coating 3 antagonistic indicator bacteria of pseudomonas, erwinia and agrobacterium on a bottom layer culture medium by adopting a double-layer plate method, pouring 1/3MB culture medium containing 2% NaCl at the temperature of about 45 ℃ on the culture medium to prepare a second layer, after the second layer is solidified, planting a bacterial strain Pontibacter sp.XAAS-72 CGMCC No.16594 on a plate, culturing at the constant temperature of 30 ℃ for 3-7d, observing and recording the antagonistic action of the bacterial strain on the indicator bacteria.
(2) The phosphorus dissolving function determination method comprises the following steps: pontibacter sp.XAAS-72 CGMCC No.16594 was inoculated on the culture medium of phosphate-solubilizing bacteria (inorganic phosphorus and organic phosphorus), and the size of the transparent circle was observed after culturing at 30 ℃ for one week.
(3) Indole acetic acid secretion test determination method: inoculating Pontibacter sp.XAAS-72 CGMCC No.16594 into TSA liquid culture medium containing 100mg/L L-tryptophan and 2% NaCl, shake culturing at 30 deg.C and 180r/min for 1d, dripping 50 μ L of the bacterial suspension onto a white ceramic plate, adding equal volume of Salkowski colorimetric solution 50mL 35% HClO4+1mL 0.5mol/L FeCl3And a mixed solution of 50. mu.L of a 2% TSA liquid medium not inoculated with a microorganism and an equal volume of a colorimetric solution was added as a control. The white ceramic plate was left at room temperature in the dark for 30min and then observed for reddening.
(4) The method for measuring the capability of producing ACC deaminase comprises the following steps: pontibacter sp.XAAS-72 CGMCC No.16594 was inoculated into DF solid medium containing 3mM ACC, and after passage for 3 times, the growth of the strain was observed on the medium containing ACC as the sole nitrogen source.
(5) Test results
The test results show in Table 1 that the bacterial strain Pontibacter sp. XAAS-72 CGMCC No.16594 has obvious antagonistic action on pseudomonas, Erwinia and Agrobacterium; in the phosphate-solubilizing test, transparent rings appear on both the culture medium for organophosphorus bacteria and the culture medium for inorganic phosphorus bacteria; in an IAA production test, the white ceramic plate is placed at room temperature in a dark place for 30min, and then the color turns red; in the test of producing ACC, Pontibacter sp.XAAS-72 CGMCC No.16594 has colony growth on the culture medium with ACC as the only nitrogen source. Test results show that the Pontibacters p.XAAS-72 CGMCC No.16594 has certain capabilities of antagonism, salt tolerance, phosphate dissolution, disease prevention and growth promotion for producing IAA and ACC.
Table 1: functional characteristics of Pontibacters p. XAAS-72 CGMCC No.16594
Example three: preparation of Pontibacers p.XAAS-72 CGMCC No.16594 bacterial agent
Pontibacter sp.XAAS-72 CGMCC No.16594 was inoculated on 1/3BD solid medium containing 2% NaCl for activation, and then inoculated on 1/3BD liquid medium containing 100mL 2% NaCl and cultured at 30 ℃ and 180rpm for 48h to obtain a seed solution. Inoculating the seed liquid into a fermentation culture medium at a ratio of 2%, and culturing at 30 ℃ and 200rpm for 96 h. Diluting the fermentation liquid with sterilized water to obtain viable bacteria with total concentration of 1 × 107CFU/mL microbial inoculum for subsequent plant growth promotion experiment microbial inoculum.
Example four: growth promoting effect of Pontibacters p.XAAS-72 CGMCC No.16594 on wheat plant
Wheat seeds with full grains and no obvious damage are selected, the test is divided into 2 groups, the test group is sterilized by 0.1 percent mercuric chloride for 5min, washed by sterile water, aired, prepared by the method provided by the third example of the previous step of preparation by using 1/100 concentration Pontibacter p.XAAS-72 CGMCC No.16594 at room temperature, and soaked for 4 h. After the control group was sterilized in the same manner, the seeds were immersed in sterilized distilled water for 4 hours.
Respectively dibbling the wheat seeds of the treated group and the wheat seeds of the control group into a hole planting pot, wherein each hole is 4 grains and the depth is about 1.5cm, and culturing in an artificial climate chamber. The culture conditions are 20 ℃, 8h without illumination, 22 ℃, 2h with 30% illumination, 12h with 100% illumination and 2h with 30% illumination. Watering once a day, and controlling the watering amount to be consistent. After germination, the seedlings are irrigated once every 5 days, and each hole is about 10 mL. And measuring the germination rate, fresh weight and plant height of the wheat plants of the test group and the control group after germination for 30 days.
The test results are shown in figure 2, figure 3 and figure 4, compared with the control treatment, the germination rate of cotton seeds soaked by the Pontibacters p.XAAS-72 CGMCC No.16594 microbial inoculum is averagely improved by 2.3 percent, and the influence is not significant. As can be seen from the attached drawings 3 and 4, after being treated by the Pontibacter sp.XAAS-72 CGMCC No.16594 microbial inoculum, the accelerating effect on the plant weight and the plant height of the wheat is obvious, and after the wheat grows for 30 days, compared with the contrast treatment, the single plant weight and the plant height of the wheat are respectively and obviously increased by 46.3 percent and 45.1 percent.
The above examples are merely illustrative for clearly illustrating the present invention and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications can be made while remaining within the scope of the present invention.
Sequence listing
<110> institute of microorganism application of Sinkiang academy of agricultural sciences (Xinjiang-Yameiya bioengineering research and development center, China)
<120> a new strain marine bacterial strain XAAS-72 and application thereof in plant antibiosis and growth promotion
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Claims (3)
1. A marine fungusPontibacterspXAAS-72, wherein saidPontibacterspThe preservation number of the strain of XAAS-72 is CGMCC No. 16594.
2. The method of claim 1PontibacterspThe gene sequence of XAAS-72 is shown in SEQ ID NO. 1.
3. The method of claim 1 or 2PontibacterspUse of XAAS-72 for promoting plant growth and controlling plant diseases.
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