CN113717903B

CN113717903B - Angelica dahurica rhizosphere klebsiella BZXI-1 and application thereof

Info

Publication number: CN113717903B
Application number: CN202111196813.9A
Authority: CN
Inventors: 吴卫; 江美彦; 刘仁浪; 刘思琴; 周杨; 杨云舒; 姚菲; 陈靳松; 杜宣; 瞿文洁; 侯凯; 冯冬菊; 陈银银
Original assignee: Sichuan Agricultural University
Current assignee: Sichuan Agricultural University
Priority date: 2021-10-14
Filing date: 2021-10-14
Publication date: 2023-05-23
Anticipated expiration: 2041-10-14
Also published as: CN113717903A

Abstract

The invention discloses a radix angelicae rhizosphere klebsiella BZXI-1, belonging to the technical field of microorganisms, wherein the radix angelicae rhizosphere klebsiella BZXI-1 is preserved in China general microbiological culture collection center (CGMCC), and the preservation number is: CGMCC No.22870, the preservation date is: 2021, 07, 09; the invention also discloses application of the angelica dahurica rhizosphere klebsiella BZXI-1, and the angelica dahurica rhizosphere klebsiella BZXI-1 provided by the invention has obvious effects of nitrogen fixation, phosphorus dissolution and indole-3-acetic acid production, so that the angelica dahurica rhizosphere klebsiella BZXI-1 can be known to be used for plant planting, especially for planting angelica dahurica and the like, can reduce the using amount of chemical fertilizers, improve the soil environment and promote plant growth, thereby being beneficial to environmental protection and green planting.

Description

Angelica dahurica rhizosphere klebsiella BZXI-1 and application thereof

Technical Field

The invention relates to the technical field of microorganisms, in particular to a radix angelicae rhizosphere klebsiella BZXI-1 and application thereof.

Background

Rhizosphere refers to soil body around plant root system affected by root system growth, and is an important place for plant root system, soil and microorganism to communicate with each other. Rhizosphere microorganisms in turn can directly or indirectly affect plant growth and health in a variety of ways, such as: mineral nutrient absorption, plant hormone production, antagonism, nutrient and space competition, soil environment improvement and induction of plant system resistance (ISR) production. Knowing the growth-promoting mechanism of rhizosphere microorganisms, actively managing soil flora may be a sustainable and effective way to reduce fertilizer pesticide application, improve soil conditions, develop natural inhibition of soil-borne diseases, and increase plant productivity.

The Chinese medicinal radix Angelicae Dahuricae is dry root of radix Angelicae Dahuricae Angelica dahurica (Fisch. Ex Hoffm.) or Hangzhou radix Angelicae Dahuricae Angelica dahurica (Fisch. Ex Hoffm.) of Umbelliferae, and is medicinal and edible, and mainly contains multiple active ingredients such as volatile oil, coumarin, and amino acids, and can be used for treating various pain symptoms in clinic, and can be used in food, chemical industry, and perfume. At present, the angelica dahurica has the problems of early bolting, atrophy of a production area, continuous use of pesticides in planting and the like, so that the yield of the angelica dahurica medicinal material is reduced, and the requirements of controllable quality, high quality and stable yield are difficult to achieve. The existing researches mainly focus on the optimization of the extraction method of the effective chemical components of the angelica dahurica, the researches on pharmacological activity and action mechanism, and the researches on the rhizosphere growth-promoting bacteria of the angelica dahurica have not been reported yet. Therefore, the angelica dahurica growth-promoting bacteria are necessary to be developed and utilized from rhizosphere, the high-yield and high-quality cultivation of the radix angelicae dahuricae is improved, and the fertilizer application is partially replaced, so that the green planting is facilitated.

In addition, the proportion of the soil N, P, K has influence on early bolting, yield and quality of the angelica dahurica. Currently, in dahurian angelica root cultivation, chemical fertilizer is generally applied to meet the growth requirement. Although the fertilizer increases the yield of radix angelicae, the fertilizer causes great artificial interference to carbon-nitrogen circulation, and the greenhouse gas N is generated ₂ The problems of environmental pollution such as increased O emission, soil acidification, eutrophication of aquatic environment and the like cause environmental pollution and are unfavorable for green planting.

Disclosure of Invention

One of the purposes of the present invention is to provide a strain of Klebsiella dahurica BZXI-1 to solve the above problems.

In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: the angelica dahurica rhizosphere klebsiella BZXI-1 is preserved in China general microbiological culture Collection center (CGMCC), and the preservation number is: CGMCC No.22870, the preservation date is: 2021, 07, 09.

The sources of the angelica dahurica rhizosphere klebsiella BZXI-1 strain are as follows: is obtained from rhizosphere soil of Angelica dahurica [ its original plant is Angelica dahurica Angelica dahurica (Fisch. Ex Hoffm) benth. Et hook. F. Var. Formosana (Boiss) Shan et Yuan ] of Umbelliferae, and is separated and screened by dilution coating plate method. Sampling: the Sichuan province is at the same time to use the Yicun county of Yicun, jixi.

The application identifies the morphology, physiological and biochemical characteristics, genetics and other angles of the strain:

morphological identification: bacteria are gram negative, motionless, straight, short rod-like, spore-free, flagellum-free, thicker, and have a capsule size of about 0.3-1.0 μm 0.6-6.0 μm (micrograph is shown in figure 1).

And (3) physiological and biochemical feature identification: is suitable for culturing on beef extract peptone culture medium, pH is 7.0-7.2, and growth is optimal at 37 ℃, and has general nutrition requirement and no need of special growth factors. The colony is round, grey white, smooth, rich and sticky on the solid culture medium, and is facultative anaerobic.

Genetic identification: the strain was subjected to 16S rDNA sequencing using 16S universal primers shown in Table 1 (namely, 27f and 1492r, respectively, SEQ ID NO:2 and SEQ ID NO: 3), and as a result, the 16S rDNA sequence was shown in SEQ ID NO: 1:

GCGCCCTCCCGAAGGTTAGCTACCTACTTCTTTTGCACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACATACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATATGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGGACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACAGCCATGCAGCACCTGTCTCACAGTTCCCGAAGGCACCAAAGCATCTCTGCTAAGTTCTCTGGATGTCAAGAGTAGGTAAGGTTCTTCGCGTTGCATCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACTCCTCAAGGGAACAACCTCCAAGTCGACATCGTTTACAGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACAAGACTCCAGCCTGCCAGTTTCGAATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCCGACTTGACAGACCGCCTGCGTGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAGGTTATTAACCTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTAGGTGAGCCGTTACCCCACCTACTAGCTAATCCCATCTGGGCACATCTGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCACCCGAGAGCAAGCTCTCTGTGCTACCGTTCGAC

the sequences were aligned by BLAST and two similar strain sequences with highest homology were found in GenBank, klebsiella grimontii (accession number: CP 056150.1) and Klebsiella oxytoca (accession number: KM 434222.1), with homology reaching 100%. For further characterization, the differential fragments of Klebsiella grimontii and Klebsiella oxytoca were sequenced with primers shown in Table 1, namely 756f, 1674r, 713f and 1962r, respectively, as SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6 and SEQ ID NO:7, respectively, and the results showed that only Klebsiella grimontii primer sequencing was successful, and the results were Klebsiella grimontii by comparison, as shown in SEQ ID NO:8:

TCAAATACCGTCAGATCGTTTTCGAATTCATATTCGTGATCCTGGGCATAGTAGCCAATCTGCGCGTTCTCGGACCATTTAACGGTACCGTTATCCGGCATCAGGTCGCCGACCAGGGTTTTCAGCATTGTGGATTTACCCACGCCGTTGGCGCCCAGAATAGCGATCTTCTCCCCCACTTCCAGCAGCAGGTTGAAGTTTTTAAACAGCGGGCCGTTGTCAAAGCCTTTTGCCAGCGCTTCCACTTCCAGAGCGTTACGGAACAGCTTCTTATCCTGCTCAAAGCGAATGAACGGGTTCTGACGGCTGGACGCTTTCACTTCGTCCAGCTTAATTTTGTCAATCTGACGGGCGCGCGAGGTCGCCTGGCGAGATTTAGAGGCGTTGGCGCTAAAGCGGCTCACGAAAGATTGCAGGTCGGCAATCTGCGCTTTTTTCTTGGCGTTATCCGCCAGCAGACGTTCGCGCGCCTGGGTCGCCGCGGTCATATATTCATCGTAGTTGCCCGGGTAAACGCGCAGCTCGCCGTAATCCAGATCCGCCATGTGGGTACAAACCATGTTCAGGAAGTGACGGTCGTGCGAAATGATGATCATGGTGCTGTCGCGATCGTTGAGGGTCTGCTCCAGCCAGCGGATGGTATCGATGTCCAGGTTGTTCGTCGGTTCATCAAGCAGCAGGATATCCGGGTTGGAGAACAGCGCCTGCGCCAGCAGCACGCGCAGCTTCCAGCCAGGAGCCACTTCGCTCATCGGGCCGTAATGCTGTTCAACCGGGATACCGACGCCGAGCAGCAGTTCGCCGGCGCGCGCTTCGGCGGAGTAGCCGTCCATCTCGCCGTACAGCACTTC

TABLE 1 primer information

The second object of the invention is to provide a microbial agent, which comprises the angelica dahurica rhizosphere klebsiella BZXI-1.

It is a further object of the present invention to provide uses of Klebsiella dahurica BZXI-1 as described above, including but not limited to use in plant cultivation for nitrogen fixation, phosphorus dissolution, indole-3-acetic acid production.

Compared with the prior art, the invention has the advantages that: the angelica dahurica rhizosphere klebsiella BZXI-1 provided by the invention has obvious nitrogen fixation, phosphorus dissolution and indole-3-acetic acid production effects, so that the angelica dahurica rhizosphere klebsiella BZXI-1 can be known to reduce the use amount of chemical fertilizers, improve the soil environment and promote the plant growth when being used for plant planting, especially the planting of angelica dahurica and the like, thereby being beneficial to environmental protection and green planting.

Drawings

FIG. 1 is a photomicrograph of Klebsiella multocida BZXI-1 of the present invention;

FIG. 2 is a photograph of nitrogen fixation medium of example 1 of the present invention;

FIG. 3 is a view showing a phosphorus dissolving ring in example 2 of the present invention;

FIG. 4 is a graph showing comparison of IAA production results in example 3 of the present invention;

FIG. 5 is a comparative chart of siderophore results of example 4 of the present invention;

FIG. 6 is a graph showing the comparison of the growth promotion results of the Chongzhou soil BZA001 according to example 5 of the present invention;

FIG. 7 is a graph showing the comparison of the growth promotion results of Chongzhou soil BZB003 in example 5 of the present invention;

FIG. 8 is a graph showing the comparison of the results of the growth promotion of the parallel soil BZA001 in example 5 of the present invention;

FIG. 9 is a graph showing the comparison of the growth promotion results of the parallel soil BZB003 in example 5 of the present invention.

Detailed Description

The invention will be further described with reference to the accompanying drawings.

Example 1:

radix angelicae rhizosphere klebsiella BZXI-1 nitrogen fixation effect experiment:

1. the experimental method comprises the following steps: activating the strain, streaking and inoculating the strain on a nitrogen fixation culture medium (the composition of the culture medium comprises 0.2g of sodium chloride, 0.2g of dipotassium hydrogen phosphate, 0.1g of calcium sulfate, 0.2g of magnesium sulfate, 2.0g of calcium carbonate, 10.0g of mannitol, 15g of agar and 1000mL of distilled water), and culturing at a constant temperature of 37 ℃ for 3 days;

2. experimental results: the continuous transfer is carried out for 3 times, the growth is good, and the nitrogen fixation capability is strong. BZXI-1 colonies were colorless and transparent water stains on nitrogen fixation medium as shown in FIG. 2.

Example 2

Radix angelicae rhizosphere klebsiella BZXI-1 phosphorus-dissolving effect experiment

1. The experimental method comprises the following steps: the strain is inoculated on an inorganic phosphorus solid plate culture medium (the composition of the culture medium is 10g of glucose, 0.5g of ammonium sulfate, 0.3g of sodium chloride, 0.3g of potassium chloride, 0.3g of magnesium sulfate heptahydrate, 0.03g of ferrous sulfate heptahydrate, 0.03g of manganese sulfate monohydrate, 0.7g of calcium phosphate, 20g of agar, 1000mL of distilled water and pH of 7.0-7.2) after being activated, and the strain is cultivated for 3 days at the constant temperature of 37 ℃;

2. experimental results: as shown in FIG. 3, transparent circles appear, which indicate that the strain BZXI-1 has phosphorus-dissolving activity, and the ratio of the diameter of the transparent circles to the diameter of colonies indicates the size of phosphorus-dissolving capacity of the strain, and D/d=2.43+ -0.01.

Example 3

Effect experiment of Angelica dahurica Klebsiella multocida BZXI-1 on indole-3-acetic acid (IAA)

1. The experimental method comprises the following steps: the strain is inoculated into beef extract peptone liquid culture medium (medium composition: beef extract 5.0g, peptone 10g, naCl 5.0g, distilled water 1000mL, pH 7.0-7.2) added with L-tryptophan 200g/mL, and cultured by shaking table at 37 ℃ for 3d at constant temperature of 180 r/min. Adopting a Salkowski colorimetric method, taking a non-inoculated culture solution as a negative control, and adding 5 mug/mL IAA as a positive control;

2. experimental results: after one hour of dark reaction, the bacterial liquid turns red, which shows that the strain BZXI-1 has the capability of producing IAA.

The absorbance was measured at 530nm using an enzyme-labeled instrument, 20. Mu.g/mL IAA standard was diluted to 5, 10, 15 and 20. Mu.g/mL, respectively, and standard curves were drawn from which strain IAA yields were calculated. The IAA production capacity was 21.96.+ -. 0.21. Mu.g/mL. As shown in FIG. 4, the negative control CK, BZXI-1 and 5. Mu.g/mLIAA positive control, respectively, were performed from left to right, i.e., IAA concentrations in the three tubes from left to right were 0, 21.96. Mu.g/mL and 5. Mu.g/mL, respectively.

Example 4

Effect experiment of Klebsiella dahurica BZXI-1 siderophore

1. The experimental method comprises the following steps: the strain is inoculated in beef extract peptone liquid culture medium (culture medium composition: 5.0g of beef extract, 10g of peptone, 5.0g of NaCl, 1000mL of distilled water, pH 7.0-7.2) and cultured for 3d by shaking at a constant temperature of 37 ℃ at 180 r/min. And (3) performing colorimetric reaction by adopting a chrome azure colorimetric method.

2. Experimental results: the color of the culture solution is changed from blue to light green, and the capability of producing the iron carrier is weak. The absorbance value was measured at 630nm with a microplate reader, and the siderophore yield was calculated to be 7.80% + -0.62% according to the formula Su= (Ar-A)/Ar X100% (where Su is siderophore content; ar is absorbance value after control reaction; A is absorbance value after bacterial liquid reaction). As shown in FIG. 5, the left side is CK, the middle and right sides are BZXI-1 bacterial solutions, and the yields of siderophores are 0%, 7.18% and 8.43% respectively.

Example 5

Root of dahurian angelica root Klebsiella rhizosphere BZXI-1 fruit test for promoting effect

1. The experimental method comprises the following steps: inoculating the strain into beef extract peptone liquid culture medium (culture medium composition: beef extract 5.0g, peptone 10g, naCl 5.0g, distilled water 1000mL, pH 7.0-7.2), shaking culture at 37deg.C for 3d at 600 rpm for 10min under 180r/min, collecting thallus, adding distilled water, shaking, and resuspension to adjust bacterial liquid concentration to OD ₅₉₅ =1, ready for use;

the variety BZA001 and the strain BZB003 of the angelica dahurica are used as test materials, and are genetically stable angelica dahurica varieties (systems) bred for many years, wherein the BZA001 is a variety of angelica dahurica of Chuanzhi No.2, and the BZB003 rhizosphere is a BZXI-1 separation source. Planting in the soil of Yu Jian village from Kangzhou city of Sichuan province (modern research and development base of Sichuan agricultural university), sowing Sichuan radix Angelicae Dahuricae used in potted plant test in Sichuan agricultural university Chengdu Qing Pu Yuan at 10 month in 2020. And selecting radix angelicae plants with consistent growth vigor in 3 months of the next year, and properly trimming dead leaf and rotten leaves to keep each plant of radix angelicae of each genotype under similar weight. Washing root of radix Angelicae Dahuricae with flowing water until no brown soil trace. Soaking radix Angelicae Dahuricae in 75% ethanol for 5min, rapidly washing with sterile water to remove residual ethanol, and repeating twice. Bacterial liquid is inoculated in each test group respectively, and is irrigated at a position 3.0cm away from the root of radix angelicae, 100mL of each strain is irrigated with an equal amount of sterile water in a control mode, and the total amount of the bacterial liquid is irrigated once a month for 3 times. Continuously culturing until the harvesting period of the radix angelicae dahuricae reaches 7 months. For each test group, 30 plants, 5 plants per pot, using round pots (d=35 cm), were used.

2. Experimental results:

the results are shown in Table 2, and in FIGS. 6-9, photographs of the medicinal materials treated with the strain BZXI-1 were taken on the left side and photographs of the medicinal materials treated with the strain BZXI-1 were taken on the right side of FIGS. 6-9. In the figure, CA is the BZA001 test group in Chongzhou soil (FIG. 6); CB is the BZB in Chongzhou soil 003 test group (fig. 7); SA is the BZA001 test group in Suiining soil (FIG. 8); SB is BZB003 test group in Chongzhou soil (FIG. 9). As can be seen from the table and the graph: the strain BZXI-1 has obvious promotion effect on the growth of radix Angelicae Dahuricae of each treatment group.

Specifically: in Suining soil, the plant height, root thickness and root fresh weight of the radix angelicae BZA001 inoculated with the strain BZXI-1 are obviously increased, and the yield is up to 31.85%; the plant height, root length, root thickness, overground fresh weight and root fresh weight of BZB003 after inoculation are obviously increased, and the yield is 64.59%. In Chongzhou soil, the plant height, the overground fresh weight and the root fresh weight of the angelica dahurica BZA001 are obviously increased, the yield is 33.57 percent, the root thickness, the overground fresh weight and the root fresh weight of the angelica dahurica BZB003 are obviously increased, and the yield is 56.73 percent. These results indicate that the strain has the effect of promoting the growth of dahurian angelica root and increasing the yield. The strain has different promotion effects on the soil of parallel or Chongzhou or between two varieties (lines), and has a certain wide applicability in the cultivation of angelica dahurica. Meanwhile, the growth promoting effect of the strain on the BZB003 angelica dahurica strain is superior to that of a BZA001 variety, which is probably derived from BZB003 rhizosphere, and can establish a good reciprocal symbiotic relationship with BZB003 so as to promote the growth of the angelica dahurica.

TABLE 2 promotion of dahurian angelica seedlings by the seed strain BZXI-1

The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the invention.

Sequence listing

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<120> A strain of Klebsiella dahurica BZXI-1 and application thereof

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ttccgacttc atggagtcga gttgcagact ccaatccgga ctacgacata ctttatgagg 180

tccgcttgct ctcgcgaggt cgcttctctt tgtatatgcc attgtagcac gtgtgtagcc 240

ctactcgtaa gggccatgat gacttgacgt catccccacc ttcctccagt ttatcactgg 300

cagtctcctt tgagttcccg gccggaccgc tggcaacaaa ggataagggt tgcgctcgtt 360

gcgggactta acccaacatt tcacaacacg agctgacgac agccatgcag cacctgtctc 420

acagttcccg aaggcaccaa agcatctctg ctaagttctc tggatgtcaa gagtaggtaa 480

ggttcttcgc gttgcatcga attaaaccac atgctccacc gcttgtgcgg gcccccgtca 540

attcatttga gttttaacct tgcggccgta ctccccaggc ggtcgactta acgcgttagc 600

tccggaagcc actcctcaag ggaacaacct ccaagtcgac atcgtttaca gcgtggacta 660

ccagggtatc taatcctgtt tgctccccac gctttcgcac ctgagcgtca gtctttgtcc 720

agggggccgc cttcgccacc ggtattcctc cagatctcta cgcatttcac cgctacacct 780

ggaattctac ccccctctac aagactccag cctgccagtt tcgaatgcag ttcccaggtt 840

gagcccgggg atttcacatc cgacttgaca gaccgcctgc gtgcgcttta cgcccagtaa 900

ttccgattaa cgcttgcacc ctccgtatta ccgcggctgc tggcacggag ttagccggtg 960

cttcttctgc gggtaacgtc aatgagcaag gttattaacc ttactccctt cctccccgct 1020

gaaagtactt tacaacccga aggccttctt catacacgcg gcatggctgc atcaggcttg 1080

cgcccattgt gcaatattcc ccactgctgc ctcccgtagg agtctggacc gtgtctcagt 1140

tccagtgtgg ctggtcatcc tctcagacca gctagggatc gtcgcctagg tgagccgtta 1200

ccccacctac tagctaatcc catctgggca catctgatgg caagaggccc gaaggtcccc 1260

ctctttggtc ttgcgacgtt atgcggtatt agctaccgtt tccagtagtt atccccctcc 1320

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gcggtcatat attcatcgta gttgcccggg taaacgcgca gctcgccgta atccagatcc 540

gccatgtggg tacaaaccat gttcaggaag tgacggtcgt gcgaaatgat gatcatggtg 600

ctgtcgcgat cgttgagggt ctgctccagc cagcggatgg tatcgatgtc caggttgttc 660

gtcggttcat caagcagcag gatatccggg ttggagaaca gcgcctgcgc cagcagcacg 720

cgcagcttcc agccaggagc cacttcgctc atcgggccgt aatgctgttc aaccgggata 780

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Claims

1. A strain of angelica dahurica rhizosphere klebsiella BZXI-1 is characterized in that: the angelica dahurica rhizosphere klebsiella BZXI-1 is preserved in China general microbiological culture Collection center (CGMCC), and the preservation number is: CGMCC No.22870, the preservation date is: 2021, 07, 09.

2. A microbial agent is characterized in that: the microbial agent comprises the angelica dahurica rhizosphere klebsiella BZXI-1 as claimed in claim 1.

3. Use of klebsiella dahurica BZXI-1 according to claim 1 for nitrogen fixation in plant cultivation.

4. Use of klebsiella dahurica BZXI-1 according to claim 1 for dissolving phosphorus in plant cultivation.

5. Use of klebsiella dahurica BZXI-1 according to claim 1 for producing indole-3-acetic acid in plant cultivation.

6. Use according to any one of claims 3-5, characterized in that: the plant is radix Angelicae Dahuricae.