CN111032682B - 用于工业生物催化的工程化转氨酶多肽 - Google Patents

用于工业生物催化的工程化转氨酶多肽 Download PDF

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CN111032682B
CN111032682B CN201880052271.8A CN201880052271A CN111032682B CN 111032682 B CN111032682 B CN 111032682B CN 201880052271 A CN201880052271 A CN 201880052271A CN 111032682 B CN111032682 B CN 111032682B
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engineered
seq
transaminase
amino acid
sequence
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CN111032682A (zh
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斯科特·J·诺维克
尼基·德拉斯
奥斯卡·阿尔维左
拉维·大卫·加西亚
沙琳·奇恩
大卫·恩特韦斯特尔
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Codexis Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y206/00—Transferases transferring nitrogenous groups (2.6)
    • C12Y206/01—Transaminases (2.6.1)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
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  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201880052271.8A 2017-06-14 2018-05-23 用于工业生物催化的工程化转氨酶多肽 Active CN111032682B (zh)

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US201762519297P 2017-06-14 2017-06-14
US62/519,297 2017-06-14
PCT/US2018/034069 WO2018231462A1 (en) 2017-06-14 2018-05-23 Engineered transaminase polypeptides for industrial biocatalysis

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CN111032682A CN111032682A (zh) 2020-04-17
CN111032682B true CN111032682B (zh) 2024-04-12

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US (3) US11359183B2 (https=)
EP (1) EP3638688A4 (https=)
JP (2) JP7122009B2 (https=)
CN (1) CN111032682B (https=)
CA (1) CA3064574A1 (https=)
IL (1) IL271114A (https=)
WO (1) WO2018231462A1 (https=)

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CN109456952B (zh) * 2018-11-30 2020-08-04 江南大学 一种可催化西他沙星五元环关键中间体的ω-转氨酶突变体
EP4133064A4 (en) 2020-04-10 2024-07-24 Codexis, Inc. Engineered transaminase polypeptides
CN112094856B (zh) * 2020-09-23 2023-06-27 国药集团威奇达药业有限公司 一种转氨酶突变体及其在西格列汀合成中的应用
EP4217502A4 (en) * 2020-09-28 2024-12-11 Codexis, Inc. Engineered biocatalysts and methods for synthesizing chiral amines
CN112430632B (zh) * 2020-11-18 2022-09-13 上海合全药物研发有限公司 2-((反式)-4-氨基环己烷基)异丙醇的制备方法
CN114645027B (zh) * 2020-12-21 2024-12-10 上海合全药物研发有限公司 一种来源于巨大芽孢杆菌的氨基转移酶突变体及其应用
CN112501145A (zh) * 2021-02-04 2021-03-16 凯莱英医药集团（天津）股份有限公司 转氨酶突变体及其应用
CN113481254B (zh) * 2021-06-29 2023-05-26 台州酶易生物技术有限公司 西他列汀中间体的制备方法
IL311268A (en) * 2021-09-15 2024-05-01 Richter Gedeon Nyrt PROCESS TO PRODUCE (1r,4r)-4-SUBSTITUTED CYCLOHEXANE-1-AMINES
CN115851649B (zh) * 2022-04-21 2024-12-03 凯莱英医药集团（天津）股份有限公司 转氨酶突变体及其应用
CN115807046A (zh) * 2022-09-13 2023-03-17 华中科技大学 一种转氨酶催化合成卡巴拉汀药物中间体的方法

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US20250250550A1 (en) 2025-08-07
US20220282229A1 (en) 2022-09-08
JP2022062113A (ja) 2022-04-19
WO2018231462A1 (en) 2018-12-20
CA3064574A1 (en) 2018-12-20
JP7212972B2 (ja) 2023-01-26
EP3638688A1 (en) 2020-04-22
CN111032682A (zh) 2020-04-17
JP7122009B2 (ja) 2022-08-19
US20200109378A1 (en) 2020-04-09
US12297462B2 (en) 2025-05-13
EP3638688A4 (en) 2021-07-07
IL271114A (en) 2020-01-30
US11359183B2 (en) 2022-06-14
JP2020523023A (ja) 2020-08-06

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