CN111012686A

CN111012686A - Tattooing cream and preparation method and application thereof

Info

Publication number: CN111012686A
Application number: CN201910348006.0A
Authority: CN
Inventors: 胡彦龙
Original assignee: Xi'an Tatu Biotechnology Co ltd
Current assignee: Xi'an Tatu Biotechnology Co ltd
Priority date: 2019-04-28
Filing date: 2019-04-28
Publication date: 2020-04-17
Anticipated expiration: 2039-04-28
Also published as: CN111012686B

Abstract

The invention belongs to the technical field of tattoo dyes, and particularly discloses a tattoo paste and a preparation method and application thereof. The tattooing paste comprises 1.0-10.0% of genipin or geniposidic acid, 0.2-2.0% of antioxidant, 1.0-5.0% of thickening agent, 5.0-10.0% of dispersing agent, 0.1-1.0% of complexing agent and 72.0-97.2% of water, wherein the complexing agent is ethylene diamine tetraacetic acid and/or disodium ethylene diamine tetraacetate. The tattooing paste disclosed by the invention avoids the influence of metal ions or non-metal ions on the texture and the coloring activity of the tattooing paste by adding the complexing agent, and effectively ensures the quality of the tattooing paste. In addition, the genipin or the geniposide acid is compounded with the thickening agent, the antioxidant, the complexing agent and the dispersing agent to prepare the paste with poor fluidity, which is convenient to operate and implement, so that the operation and the implementation of tattooing are facilitated, and the quality guarantee period of the tattooing paste is effectively prolonged.

Description

Tattooing cream and preparation method and application thereof

Technical Field

The invention belongs to the technical field of tattoo dyes, and particularly relates to a tattoo paste and a preparation method and application thereof.

Background

Tattoos have been part of human culture for over two thousand years, and recently, as society develops, tattoos are becoming popular among young people. The traditional tattooing method is to inject tattooing fluid into the dermal skin layer by a needle or similar tool to make the dye adhere to the skin and display colored patterns, on one hand, the tattooing method needs professional personnel to operate in clean environment or by using sterile equipment, which is expensive, and the tattooing process is often accompanied with skin injury, which may cause infection or cause unrecoverable skin injury; on the other hand, after the tattoo is finished by the method, the tattoo image is difficult to change or remove, and the tattoo is usually removed by a laser or an operation method, which causes secondary damage to the skin of the human body.

In recent years, there is a trend toward temporary tattooing methods, in which a tattoo liquid such as impatiens balsamina or other tattoo liquid having a coloring effect is applied to the skin of a human body to form a color on the skin surface for a certain period of time. Chinese patent application publication No. CN1537900A discloses an ink suitable for tattooing, which is composed of a colorant, a resin, a surfactant, a thickener and a solvent, but the ink forms a colored pattern only by attaching a pigment to the skin surface, has the defects of poor water resistance, poor attachment fastness and short lasting of tattooing effect, and the pigment constituting the ink may contain heavy metal ions or aromatic amines, etc., which may cause uncomfortable symptoms such as skin allergy, itching, rash, pain, etc., and a method for preparing and using the same. Chinese patent publication No. CN101781530A discloses a self-adhesive tattoo sticker and a method for preparing the same, wherein 0.1-50% of genipin or genipin acid is mixed with 50-99.9% of adhesive, the mixture is coated on the surface of a support, and cut to obtain a sticker with a specific shape, the sticker is attached to the surface of skin when in use, and the coloring component genipin or genipin acid in the mixture reacts with amino compound on the surface of skin to achieve tattooing effect, however, the sticker has a certain defect, firstly, during the preparation process of the sticker, a genipin or genipin acid sample is directly mixed with the self-adhesive glue, because genipin or genipin acid can be dissolved in organic solvents such as methanol and ethanol, and the direct mixing with the self-adhesive glue can hardly make the self-adhesive glue or the self-adhesive glue mix uniformly, resulting in poor coloring effect or non-uniform coloring degree; secondly, the processes of coating genipin or a geniposidic acid-adhesive mixture, cutting a sticker and the like need specific equipment, and the process is complex; thirdly, the mixture is easily polluted in the coating process of the genipin or the geniposide-adhesive mixture or the cutting and storing process of the paster loaded with the mixture, or the mixture is dried or the coloring component is oxidized and decomposed due to improper storage of the paster, because genipin and geniposide are mostly plant-extracted active substances, the mixture is sensitive to illumination and temperature and is easily affected by the external environment to lose activity, so that the tattooing effect of the self-adhesive tattooing paster is poor, and even the color cannot be developed.

Disclosure of Invention

In view of the above-mentioned deficiencies of the prior art, the present invention aims to provide a tattooing paste with uniform coloring effect, strong durability and clear pattern.

As a first aspect of the present invention, the present invention provides a tattooing paste.

Preferably, the tattooing paste comprises 1.0-10.0% of genipin or geniposide acid, 0.2-2.0% of antioxidant, 1.0-5.0% of thickening agent, 5.0-10.0% of dispersing agent and 72.0-97.2% of water.

Preferably, the tattooing paste further comprises 0.1-1.0% of a complexing agent.

Preferably, the complexing agent is ethylene diamine tetraacetic acid and/or disodium ethylene diamine tetraacetic acid.

Preferably, the antioxidant consists of 0.1-1.0% of water-soluble antioxidant and 0.1-1.0% of alcohol-soluble antioxidant.

Preferably, the water-soluble antioxidant is sodium sulfite or sodium pyrosulfite, and the alcohol-soluble antioxidant is methyl hydroxybenzoate, ethyl hydroxybenzoate or propyl hydroxybenzoate.

Preferably, the thickener is any one or more of xanthan gum, sodium carboxymethylcellulose, gum, guar gum, konjac gum, arabinogalactan, pectin and amylopectin.

The plurality can be any two, three, or four and the like.

Preferably, the dispersant is ethanol.

As a second aspect of the present invention, the present invention provides a method for preparing a tattooing paste.

Preferably, the tattooing paste comprises 1.0-10.0% of genipin or geniposide acid, 0.2-2.0% of antioxidant, 1.0-5.0% of thickening agent, 5.0-10.0% of dispersing agent, 0.1-1.0% of complexing agent and 72.0-97.2% of water.

Preferably, the preparation of the tattooing paste comprises the following steps:

1) dissolving genipin or geniposide acid and alcohol-soluble antioxidant in dispersant;

2) dissolving a water-soluble antioxidant and a complexing agent in water to obtain an aqueous solution;

3) dispersing a thickening agent in the solution obtained in the step 1) to obtain a mixed solution;

4) adding the aqueous solution obtained in the step 2) into the mixed solution obtained in the step 3), and uniformly stirring to obtain the tattoo paste.

Preferably, the dispersant is ethanol.

As a third aspect of the invention, the invention provides a tattoo kit.

Preferably, the tattoo kit comprises:

1) the tattooing paste comprises 1.0-10.0% of genipin or geniposidic acid, 0.2-2.0% of antioxidant, 1.0-5.0% of thickening agent, 5.0-10.0% of dispersing agent, 0.1-1.0% of complexing agent and 72.0-97.2% of water, and is packaged in a dark plastic soft bottle with a needle head;

2) the PVC sticker, the PVC sticker includes adhesion layer and slip sheet, hollow out pattern has on the adhesion layer, the thickness on adhesion layer is 0.1~ 0.3 mm.

Preferably, the use method of the tattoo set comprises the following steps:

1) adhering an adhesive layer of the PVC sticker to the surface of the skin, wherein the adhesive layer forms a cavity with the thickness of 0.1-0.3 mm on the surface of the skin corresponding to the hollowed-out pattern;

2) smearing the tattooing ointment on the exposed skin in the cavity, removing the tattooing ointment after the tattooing ointment is dried, and coloring the skin.

Preferably, after the tattoo paste is removed, the color development can be accelerated by a heat treatment mode, wherein the heat treatment mode comprises hot compress, hot air blowing or light treatment on the skin to be tattooed, and the heat treatment temperature is 40-50 ℃.

Preferably, the dispersant is ethanol.

Advantageous effects

(1) The tattooing paste disclosed by the invention avoids the influence of metal ions or non-metal ions on the texture and the coloring activity of the tattooing paste by adding the complexing agent, so that the quality and the tattooing effect of the tattooing paste are effectively ensured, and the prepared tattooing paste is safe and reliable.

(2) The genipin or the geniposide acid is compounded with the thickening agent, the antioxidant, the complexing agent and the dispersing agent to prepare the paste with poor fluidity, which is convenient for operation and implementation, and is convenient for storage.

(3) The tattooing paste can play a role in remarkably protecting genipin and geniposidic acid by compounding the water-soluble antioxidant sodium sulfite or sodium metabisulfite and the alcohol-soluble oil antioxidant ethylparaben, not only ensures the coloring activity of the tattooing paste, but also effectively prolongs the storage period of the tattooing paste, and can be stored for more than 6 months at normal temperature and more than 12 months at low temperature.

(4) When the tattoo paste is prepared, ethanol is used as a dispersing agent, on one hand, the thickening agent is firstly dispersed in an ethanol solution, and then the aqueous solution is added into the ethanol solution dispersed with the thickening agent, so that the problem that the thickening agent can form a large amount of micelle because the thickening agent is directly dissolved in water in the traditional method is effectively avoided.

(5) The tattoo paste has the advantages of uniform texture, gorgeous and uniform coloring, tattoo effect lasting for 20-30 days, stable and durable coloring, no irritation, good compatibility with human bodies and the like, and the addition amount of genipin or genipin acid of the tattoo paste is 1.0-10.0 percent, which is obviously lower than that of the existing tattoo paste, so that the cost is saved.

(6) The invention provides a tattoo suit, wherein a PVC sticker in the tattoo suit has a certain thickness, so that a cavity with a specific shape can be formed when the PVC sticker is pasted on the surface of the skin, the cavity is used for containing tattoo liquid on one hand, and the other side is used for indicating the coating thickness of the tattoo liquid on the other hand. The invention can ensure that the effective components of the tattooing cream are not influenced by subpackaging the tattooing cream and the PVC paster, ensure the quality and the tattooing effect of the tattooing cream, and facilitate the circulation and the transportation of the tattooing cream by adopting the tattooing suit of the invention.

(7) The tattoo cream is prepared from common raw materials in cosmetics, the addition amount is controlled within a range acceptable by human bodies, no damage is caused to the skin, no obvious skin symptoms such as erythema or wheal and the like appear in the using process, and the tattoo cream has the advantages of safety, reliability and good general applicability.

Drawings

FIG. 1 is a diagram of a possible reaction mechanism of genipin and nitrogen-containing compounds.

FIG. 2 is a schematic diagram showing the coating effect of the tattooing paste according to the present invention and commercially available pigment-based tattooing pastes.

FIG. 3 is a schematic diagram showing the effects of the tattooing paste of the present invention and commercially available pigment-based tattooing pastes after coating.

FIG. 4 is a schematic diagram showing the effect of tattooing the tattooing cream of the present invention and commercially available pigment-based tattooing cream on day 2.

FIG. 5 is a schematic diagram showing the effect of tattooing on day 3 of the tattooing paste of the present invention and commercially available pigment-based tattooing paste.

FIG. 6 is a schematic diagram showing the effect of tattooing on day 4 of the tattooing paste of the present invention and commercially available pigment-based tattooing paste.

FIG. 7 is a schematic diagram showing the effect of tattooing on day 5 of the tattooing paste of the present invention and commercially available pigment-based tattooing paste.

FIG. 8 is a schematic view showing the effect of tattooing the tattooing cream according to the present invention on day 10.

FIG. 9 is a schematic view showing the effect of tattooing the tattooing cream of the present invention on day 20.

Detailed Description

Genipin and genipin acid

Genipin (Genipin, C)₁₁H₁₄O₅) The genipin has very active chemical properties, and when combined with compounds with amino groups (such as amino acids, collagen, chitosan, glucamine compounds, various proteins and enzymes), the genipin can react to form a safe, reliable, non-toxic and blue natural pigment-blue pigment, wherein the blue natural pigment-blue pigment has a possible reaction mechanism that genipin is firstly attacked by an amino nucleophiles of the Geniposide compound to generate a ring opening reaction with the compounds, and the compound reacts with the genipin to form a blue natural pigment-blue pigment, wherein the blue natural pigment-blue pigment is usually used as a nitrogen-containing cross-linked food compound (a nitrogen-containing cross-linked plant dye compound is formed by a nitrogen-containing plant dye monomer, and a nitrogen-containing cross-linked plant dye monomer is usually used for forming a nitrogen-containing compound, and the compound is used for detecting a nitrogen-containing compound in a medicine.

Genipin acid (Genipin acid, C)₉H₁₂O₄) Is a product obtained by carrying out deesterification and hydrolysis on geniposide, or is obtained by hydrolyzing genipin under an alkaline condition, and can react with nitrogen-containing compounds such as protein or amino acid to generate a red natural pigment, namely gardenia red pigment.

The gardenia blue pigment and the gardenia red pigment have stronger coloring capability and stability, can keep the relative stability of color generation in a larger pH range, and are also relatively stable to heat and illumination. According to the invention, genipin and geniposide acid respectively generate a crosslinking reaction with nitrogen-containing compounds such as protein on the surface of the skin, and crosslinked gardenia blue pigment or gardenia red pigment is generated on the surface layer of the skin, so that the tattooing effect is realized. The gardenia blue pigment or gardenia red pigment is an intermolecular and intramolecular cross-linked structure formed by genipin or geniposidic acid and nitrogen-containing substances on the surface layer of the skin, so that the gardenia blue pigment or gardenia red pigment has stronger coloring stability and coloring durability.

The genipin and the geniposide acid used in the present invention may be prepared by extraction from natural plants or by chemical synthesis methods known in the art, and preferably the genipin and the geniposide acid used are derived from plant-extracted substances.

Complexing agents

Metal ions, even heavy metal ions such as mercury, lead, cadmium and the like, may be inevitably introduced in the preparation and storage processes of the tattoo paste, for example, metal ions may be contained in raw materials constituting the tattoo paste, or metal ions may be introduced in the equipment and operation processes used in the preparation process, and the metal ions may react with active substances, genipin and geniposic acid, so that the coloring activity of genipin or geniposic acid is reduced, and the tattoo paste may be agglomerated or precipitated in the storage process. In addition, if heavy metal ions exist in the tattooing paste, the safety of the product cannot be guaranteed. The complexing agent can effectively remove metal ions and heavy metal ions, can be used for forming a stable system, prevents the tattoo paste from flocculating, agglomerating and discoloring, and reduces coloring difference.

Ethylene diamine tetraacetic acid and disodium ethylene diamine tetraacetate are two commonly used excellent complexing agents, have very high stability constants, they can produce stable complex with most metal ions except alkali metal, eliminate metal ions or harmful effects caused by metal ions, and can also play a role in antioxidation, anticorrosion, color protection, dispersion, and are favorable for protecting genipin and geniposide acid.

Thickening agent

The thickening agent is added into the tattooing paste, so that on one hand, the viscosity of the tattooing paste is adjusted, the genipin or the genipin acid is matched with the thickening agent to prepare the paste with poor fluidity, the tattooing paste can be easily formed into a film on the surface of the skin, the adhesive force between the tattooing paste and the skin is increased, and the tattooing operation and the preservation of the tattooing paste are facilitated. In addition, the thickening agent also has dispersing and stabilizing effects, and is beneficial to preventing flocculation or precipitation of the tattoo paste during storage.

The thickener having an appropriate concentration can improve the viscosity of the tattooing paste, but if the viscosity of the tattooing paste is too high, it is difficult to dry or not naturally dry on the skin surface, and the manipulation of tattooing is affected, so the amount of the thickener added is preferably 1.0 to 2.0%.

Various types of thickeners commonly used in the art are suitable for use in the present invention, but it is desirable that the thickener not be a compound having a primary amine group that is capable of reacting with genipin or geniposidic acid, such as chitosan, gelatin, and the like. Thickeners that may be used include xanthan gum, sodium carboxymethylcellulose, gums, guar gum, konjac gum, arabinogalactans, pectins, pullulans, and the like. The xanthan gum is a biological gum with excellent performance, has low-concentration and high-viscosity characteristics, has good stability on acid, alkali and enzymolysis, and is easy to form transparent colloidal solution by taking sodium carboxymethyl cellulose as a cellulose thickening agent. The thickening agent is often applied to the food field and the cosmetic field for improving the performance of food or cosmetics, has high safety, has no irritation when being applied to the tattooing cream, and does not cause discomfort and allergy symptoms such as skin itch, pain and the like.

Antioxidant agent

The genipin and the geniposide acid have instability, the solution of the genipin and the geniposide acid is easy to be subjected to oxidative decomposition, and the solution can be subjected to oxidative decomposition at room temperature for 1 week, so that the coloring activity is lost. Sodium sulfite and sodium metabisulfite are two commonly used water-soluble antioxidants and have significant antioxidant and preservative effects. Methyl hydroxybenzoate, ethylparaben and propylparaben are three common grease antioxidants and preservatives, have wide antibacterial effects on mould, yeast and bacteria, have the characteristics of strong antibacterial power, low toxicity and no irritation, and are widely applied to cosmetics. The invention discovers that the compound of the water-soluble antioxidant and the alcohol-soluble grease antioxidant can obviously inhibit the oxidative decomposition of the geniposide and the geniposide in the tattooing paste, ensure the coloring activity of the tattooing paste and effectively prolong the preservation period of the tattooing paste.

Dispersing agent

The purpose of adding the dispersant to the tattoo cream of the present invention is to improve coloring ability and coloring uniformity and prevent the tattoo cream from flocculating and settling. The dispersant classes include fatty acids, fatty amides, and esters, however, neither dispersant is suitable for use in the present invention. First, the dispersant cannot be a compound having a primary amine group that can react with genipin or geniposidic acid.

The invention finds that ethanol is used as a dispersing agent to have good dispersing effect, and when the ethanol is used as the dispersing agent, other dispersing agents are not required to be added, so that the obtained tattooing paste has good performance. The inventor finds that a large amount of micelles can be formed if the thickening agent is directly added into water in the preparation process of the tattoo cream, the micelles cannot be completely dissolved, and the tattoo cream is very unfavorable for preparation. The thickening agents used in the present invention are in the form of powders such as xanthan gum and sodium carboxymethylcellulose, which are extremely hydrophilic, and if added directly to water, the outer layer absorbs water and swells into a micelle, preventing water from entering the inner layer, which lowers its solubility in water. In the present invention, when the thickener is added to ethanol, since the thickener is insoluble in ethanol and is dispersed in ethanol to form individual fine particles, at this time, no micelle is formed by adding an aqueous solution, and thus the tattooing cream prepared is uniform in texture without agglomeration.

On the other hand, ethanol is also used as a solvent in the present invention for improving the solubility of genipin and geniposidic acid, and for dissolving alcohol-soluble substances. On the other hand, ethanol can soften the stratum corneum of the skin, which is beneficial for the contact of the coloring active substance in the tattoo paste with the skin, thereby being beneficial to enhancing the coloring effect and shortening the color development time. In the present invention, the ethanol used is absolute ethanol.

Detailed Description

The present invention will be described in more detail with reference to examples. It is to be understood that the practice of the invention is not limited to the following examples, and that any variations and/or modifications may be made thereto without departing from the scope of the invention.

In the invention, all parts and percentages are weight units, and all equipment, raw materials and the like can be purchased from the market or are commonly used in the industry, if not specified. Unless otherwise indicated, the examples employ methods that are within the ordinary skill in the art. In the present invention, genipin and geniposide acid are used from shin-biology, Linchuan, and the pigment tattooing cream used is commercially available Haina tattooing cream.

Example 1

In this example, the inventors studied the effect of two metals on the coloring and storage properties of a tattooing paste consisting of 3.0% genipin, 1.0% ethylparaben, 1.0% sodium sulfite, 3.0% xanthan gum, 5.0% ethanol, and the balance water, wherein 10% Fe was added to the tattooing paste of experimental group 1³⁺10% Cu was added to the tattooing cream of Experimental group 2²⁺And tattooing cream without metal ions is used as a reference. The tattooing cream is tried on arms respectively, tattooing effect is observed, and the film coating amount of the tattooing cream is kept consistent. Meanwhile, the tattooing cream is placed at room temperature for 1 month, the state of the cream is observed, and the experimental results are shown in the following table:

TABLE 1 coloring effect and paste status

As a result of the above, it was found that a certain concentration of metal ions, such as iron ions and copper ions, has an influence on the coloring property and stability of the tattooing paste, and we hypothesize that it may be that the metal ions react with genipin to degrade the coloring property and cause the tattooing paste to agglomerate or precipitate during storage, resulting in uneven coloring effect.

Example 2

The tattooing paste may inevitably introduce metal ions during the preparation process, and based on the experimental results of example 1, the inventors conceived to ensure the quality of the tattooing paste as much as possible by adding a certain amount of complexing agent to the tattooing paste.

In this example, the tattooing paste consists of 3.0% genipin, 1.0% ethylparaben, 1.0% sodium sulfite, 3.0% xanthan gum, 5.0% ethanol, and the balance of water, wherein 10% Fe is added to the tattooing paste of experimental group 1³⁺The tattooing ointment of the experimental group 2 is added with 0.05 percent of disodium ethylene diamine tetraacetate on the basis of the experimental group 1, the tattooing ointment of the experimental group 3 is added with 0.1 percent of disodium ethylene diamine tetraacetate on the basis of the experimental group 1, the tattooing ointment of the experimental group 4 is added with 1.0 percent of disodium ethylene diamine tetraacetate on the basis of the experimental group 1, the tattooing ointment of the experimental group 5 is added with 1.0 percent of disodium ethylene diamine tetraacetate on the basis of the experimental group 1, and the tattooing ointment without complexing agent and metal ions is used as a control. The tattooing cream is tried on arms respectively, tattooing effect is observed, and the film coating amount of the tattooing cream is kept consistent. Meanwhile, the tattooing cream is placed at room temperature for 2 months, the condition of the cream is observed, and the experimental results are shown in the following table:

TABLE 2 coloring effect and paste status

From the experiments, the effect of the metal ions on the coloring effect and the paste condition of the tattooing paste can be effectively avoided after 0.1-1.0% of the complexing agent is added into the tattooing paste added with the metal ions, which shows that the effect of the metal ions on the tattooing paste can be avoided after the complexing agent is added into the tattooing paste, and the quality of the tattooing paste is ensured.

Example 3

A tattoo paste is prepared by the following steps:

dissolving 1.0% genipin and 0.1% ethylparaben in 5.0% ethanol to obtain an alcoholic solution, dissolving 0.1% sodium sulfite and 0.1% disodium edetate in 92.7% water to obtain an aqueous solution, dispersing 1.0% xanthan gum in the alcoholic solution, slowly adding the aqueous solution into the alcoholic solution in which the xanthan gum is dispersed, and uniformly stirring to obtain the tattooing paste.

Example 4

A tattoo paste is prepared by the following steps:

dissolving 5.0% genipin and 0.5% ethylparaben in 8.0% ethanol to obtain an alcoholic solution, dissolving 0.5% sodium sulfite and 0.5% disodium edetate in 82.5% water to obtain an aqueous solution, dispersing 3.0% xanthan gum in the alcoholic solution, slowly adding the aqueous solution into the alcoholic solution in which the xanthan gum is dispersed, and uniformly stirring to obtain the tattooing paste.

Example 5

A tattoo paste is prepared by the following steps:

dissolving 10.0% genipin and 1.0% ethylparaben in 10.0% ethanol to obtain an alcohol solution, dissolving 1.0% sodium sulfite and 1.0% disodium ethylene diamine tetraacetate in 72.0% water to obtain an aqueous solution, dispersing 5.0% xanthan gum in the alcohol solution, slowly adding the aqueous solution into the alcohol solution in which the xanthan gum is dispersed, and uniformly stirring to obtain the tattooing paste.

Example 6

A tattoo paste is prepared by the following steps:

dissolving 10.0% genipin and 1.0% ethylparaben in 10.0% ethanol to obtain an alcoholic solution, dissolving 1.0% sodium metabisulfite and 1.0% disodium ethylene diamine tetraacetate in 72.0% water to obtain an aqueous solution, dispersing 5.0% sodium carboxymethylcellulose in the alcoholic solution, slowly adding the aqueous solution into the alcoholic solution in which the carboxymethylcellulose is dispersed, and uniformly stirring to obtain the tattooing paste.

Example 7

A tattoo paste is prepared by the following steps:

10.0% of genipin and 1.0% of ethylparaben are dissolved in 5.0% of ethanol to obtain an alcoholic solution, 1.0% of sodium sulfite and 1.0% of ethylenediamine tetraacetic acid are dissolved in 77.0% of water to obtain an aqueous solution, 5.0% of a thickening agent consisting of xanthan gum and sodium carboxymethylcellulose in a mass ratio of 1:1 is dispersed in the alcoholic solution, and then the aqueous solution is slowly added into the alcoholic solution in which the thickening agent is dispersed and uniformly stirred to obtain the tattooing paste.

Example 8

A tattoo paste is prepared by the following steps:

dissolving 1.0% geniposide and 1.0% ethylparaben in 5.0% ethanol to obtain an alcoholic solution, dissolving 1.0% sodium metabisulfite and 1.0% disodium edetate in 86.0% water to obtain an aqueous solution, dispersing 5.0% xanthan gum in the alcoholic solution, slowly adding the aqueous solution into the alcoholic solution in which the xanthan gum is dispersed, and uniformly stirring to obtain the tattooing cream.

Example 9

A tattoo paste is prepared by the following steps:

dissolving 5.0% of geniposide and 1.0% of ethylparaben in 10.0% of ethanol to obtain an alcoholic solution, dissolving 1.0% of sodium sulfite and 1.0% of ethylenediamine tetraacetic acid in 77.0% of water to obtain an aqueous solution, dispersing 5.0% of a thickening agent consisting of xanthan gum and sodium carboxymethylcellulose in a mass ratio of 1:1 in the alcoholic solution, slowly adding the aqueous solution into the alcoholic solution in which the thickening agent is dispersed, and uniformly stirring to obtain the tattooing paste.

Example 10

In order to achieve convenient and simple operation of tattooing, the present embodiment provides a tattooing set, which comprises the following components:

(1) tattooing paste: the tattooing paste comprises 1.0% of genipin, 1.0% of xanthan gum, 0.1% of sodium sulfite, 0.1% of ethylparaben, 0.1% of disodium ethylene diamine tetraacetate, 5% of ethanol and 92.7% of water; the preparation method of the tattooing paste is as described in example 1, and the tattooing paste is packaged in dark plastic soft bottles with needles;

(2) PVC paster: the PVC sticker includes adhesion layer and slip sheet, the last fretwork pattern that has of adhesion layer, the thickness on adhesion layer is 0.1 mm.

In the embodiment, the tattooing paste is separately filled in a dark plastic soft bottle with a needle head for the purpose of convenient storage and transportation, on one hand, the tattooing paste is in a sealed and light-proof storage state before use, and each component can not be influenced by the external storage environment, especially, genipin or genipin acid can not be oxidized and decomposed or irradiated and can ensure good quality and tattooing effect of the tattooing paste; on the other hand, when in use, the tattooing paste can be released from the needle head by extruding the plastic soft bottle, thereby being convenient for operation. Any dark colored plastic soft bottle with a needle commonly used in the art, such as brown, brown or black soft bottles, can be used in the present invention, and a preferred form is shown in appearance patent CN 201730454811.3.

In the embodiment, the PVC sticker is a form commonly used in the field, and the adhesion layer of the PVC sticker is provided with an adhesive and can be adhered to the surface of the skin; the PVC sticker has a specific hollowed-out pattern on an adhesive layer, and the pattern is prepared by a cutting machine or a carving machine; the lining paper and the adhesive layer are adhered together to protect the adhesive on the adhesive layer, and when the adhesive is used, the lining paper is removed, and the adhesive layer is adhered to the surface of skin needing tattooing. When the adhesion layer of the PVC sticker is stuck on the surface of the skin, the skin at the hollow pattern is exposed, and at the moment, the adhesion layer of the PVC sticker has a certain thickness, so that a cavity with a specific pattern is formed on the skin by the adhesion layer, and the tattooing can be realized by smearing the tattooing paste on the exposed skin in the cavity. For general consumers, it is not always clear how much the tattoo paste should be applied properly, the amount of applied tattoo paste is too small, which results in lighter color, too much applied tattoo paste, and unnecessary waste, the cavity formed on the skin surface by the PVC sticker of the present invention is used for accommodating tattoo paste on one hand, and is used for indicating the amount of applied tattoo paste on the other hand, which avoids too much or too little applied tattoo paste, and the inventor has found through experiments that when the thickness of the adhesive layer of the PVC sticker is 0.1-0.3 mm, that is, when the height of the cavity with a specific pattern formed on the skin from the skin surface is 0.1-0.3 mm, the cavity is filled with tattoo paste, and the obtained tattoo effect is optimal. This provides convenient guidance to the consumer in controlling the amount of tattoo paste applied to achieve the best tattoo results.

The use method of the tattoo suit of the embodiment comprises the following steps: removing the lining paper from the PVC sticker, attaching the adhesive layer to the clean skin surface, smearing the tattooing paste in a cavity formed by the adhesive layer and the skin surface, removing the adhesive layer and the tattooing paste from the skin surface after the tattooing paste is dried on the skin surface (about 10-30 min), and displaying a black-blue pattern on the skin after 3 hours.

Example 11

A tattooing kit, said tattooing kit comprising:

(1) tattooing paste: the tattooing paste comprises 10.0% of geniposide, 5.0% of sodium carboxymethylcellulose, 1.0% of sodium metabisulfite, 1.0% of ethylparaben, 1.0% of disodium ethylene diamine tetraacetate, 10.0% of ethanol and 72.0% of water; the preparation method of the tattooing paste is as described in embodiment 1, and the tattooing paste is packaged in dark plastic soft bottles with needles;

(2) PVC paster: the PVC sticker includes adhesion layer and slip sheet, the last fretwork pattern that has of adhesion layer, the thickness on adhesion layer is 0.3 mm.

The use method of the tattoo suit of the embodiment comprises the following steps: removing the lining paper from the PVC sticker, attaching the adhesive layer to the clean skin surface, smearing the tattooing paste in a cavity formed by the adhesive layer and the skin surface, removing the adhesive layer and the tattooing paste from the skin surface after the tattooing paste is dried on the skin surface, and displaying a red pattern on the skin after 3 hours.

The use method of the invention is convenient to operate, the consumer can realize tattooing without professional personnel, and the subpackaging of the tattooing paste and the PVC sticker can ensure that the effective components of the tattooing paste are not affected and the tattooing effect of the tattooing paste is ensured. And, a tattoo suit may include different PVC stickers having various patterns and tattoo pastes including genipin or geniposic acid, so that consumers can select patterns and tattoo colors according to their personal preferences, select a tattoo paste including genipin if a blue pattern is desired, select a tattoo paste including genipin if a red pattern is desired, apply a tattoo paste including genipin on a corresponding portion according to their personal preferences, and apply a tattoo paste including genipin on another corresponding portion according to their personal preferences. The method of the invention provides more selectivity and autonomy for consumers, and obtains the approval and the love of a large number of consumers.

Comparative example 1

A tattoo paste is prepared by the following steps:

mixing 10.0% genipin and 90.0% non-setting adhesive glue, and stirring to obtain tattooing paste.

Comparative example 2

A tattoo paste is prepared by the following steps:

dissolving 1.0% genipin and 0.1% ethylparaben in 5.0% ethanol to obtain an alcoholic solution, dissolving 0.1% sodium sulfite in 92.8% water to obtain an aqueous solution, dispersing 1.0% xanthan gum in the alcoholic solution, slowly adding the aqueous solution into the alcoholic solution in which the xanthan gum is dispersed, and uniformly stirring to obtain the tattooing paste.

Comparative example 3

A tattoo paste is prepared by the following steps:

dissolving 1.0% genipin, 5.0% water-soluble acrylic resin and 0.1% disodium ethylene diamine tetraacetate in 92.9% water to obtain an aqueous solution, slowly dissolving 1.0% xanthan gum in the aqueous solution, and uniformly stirring to obtain the tattoo paste.

Comparative example 4

A tattoo paste is prepared by the following steps:

dissolving 1.0% genipin and 0.1% ethylparaben in 5.0% ethanol to obtain an alcoholic solution, dissolving 0.1% disodium edetate in 92.8% water to obtain an aqueous solution, dispersing 1.0% xanthan gum in the alcoholic solution, slowly adding the aqueous solution into the alcoholic solution in which the xanthan gum is dispersed, and uniformly stirring to obtain the tattooing paste.

Comparative example 5

A tattoo paste is prepared by the following steps:

dissolving 1.0% genipin in 5.0% ethanol to obtain an alcohol solution, dissolving 0.1% sodium sulfite and 0.1% disodium ethylene diamine tetraacetate in 92.8% water to obtain an aqueous solution, dispersing 1.0% xanthan gum in the alcohol solution, slowly adding the aqueous solution into the alcohol solution in which the xanthan gum is dispersed, and uniformly stirring to obtain the tattooing paste.

Example 12

Test for sensitivity test of tattooing pastes prepared in examples 3 to 9.

The tattooing cream of examples 3-9 was subjected to a smear trial test, wherein 14 healthy subjects with age range of 25 to 40 years old were selected and randomly divided into 7 groups, and the two subjects were one group, and all subjects had healthy and smooth arm skin, no erythema, desquamation and other skin rash, and no other skin diseases or systemic diseases. Before the test, each subject signs an informed consent, after the skin is cleaned, the tattoo cream prepared in the embodiment 3-9 is respectively smeared on the skin of an arm, the skin is observed once per hour, whether erythema and edema are formed is determined, and no erythema and edema appear on the skin surface of all the subjects within 12 hours.

Example 13

Test for coloring effect and stability of tattooing pastes prepared in examples 3-9 and comparative examples 1-5.

33 SD rats of 10 weeks old cleaning grade are purchased from the animal experiment center of Shanxi Chinese medicine university, the male and female are unlimited and randomly divided into 11 groups of 3 rats, and 2 multiplied by 2cm is selected from the backs of the rats²The area (2) is removed of body hair, and the epidermis is not damaged by attention, so that the skin is completely leaked. The tattooing pastes prepared in the above examples and comparative examples were applied on the skin with leakage, respectively, and the skin was covered with a common feedFeeding, temperature of feeding (20 ℃. + -. 2 ℃), humidity 60%. + -. 5%, skin colour change was recorded daily until the colour completely disappeared, and the number of days for maintenance of colour was averaged and the results are shown in the following table:

TABLE 3 tattoo cream coloring stability test results

Group of	Coloring effect	Days of maintenance of staining
			Example 3	Showing dark blue color, uniform coloring and bright color	26
Example 4	Showing dark blue color, uniform coloring and bright color	25
			Example 5	Showing dark blue color, uniform coloring and bright color	28
Example 6	Showing dark blue color, uniform coloring and bright color	23
			Example 7	Showing dark blue color, uniform coloring and bright color	27
Example 8	Red, uniform and bright color	22
			Example 9	Red, uniform and bright color	27
Comparative example 1	Lighter and uneven coloring	5
			Comparative example 2	Showing dark blue color, uniform coloring and bright color	24
Comparative example 3	Black blue and uneven coloring	20
			Comparative example 4	Showing dark blue color, uniform coloring and bright color	21
Comparative example 5	Showing dark blue color, uniform coloring and bright color	20

As can be seen from the above results, the tattooing paste according to the present invention is colored uniformly, and is bright in color, and the tattooing effect and the coloring stability are significantly higher than those of the comparative examples. The comparative example 1 is prepared by directly mixing a genipin sample and non-setting adhesive glue, the tattooing paste prepared by the method has uneven texture, and the genipin can not be completely dissolved in the non-setting adhesive glue to obtain the paste, so the coloring effect is poor, and the coloring maintenance days are short. Comparative example 3 using a water-soluble acrylic resin as a dispersant, it was found that the dispersion effect was not as good as the tattoo paste according to the present invention, resulting in uneven coloring. The tattooing pastes of comparative examples 2-5 had insignificant difference in coloring stability from examples 3-9.

Example 14

Stability test experiment of tattooing pastes prepared in example 3 and comparative examples 1 to 5.

After the tattoo pastes prepared in example 3 and comparative examples 1-5 were stored at room temperature for 6 months, the surface state of the tattoo paste was observed, and the coloring effect of the tattoo paste after being left for 6 months was measured by the method described in example 13, and the experimental results are shown in the following table:

TABLE 4 tattoo cream stability test results

From the above results, it can be seen that the surface condition of the tattooing paste according to the present invention does not change significantly after 6 months of storage at room temperature, and still has significant coloring effect, whereas the tattooing paste according to comparative example 1 has significantly changed texture, darkened color of the paste, and a large amount of micelles appeared, and it was found that coloring was not possible after application, indicating that the tattooing paste was deteriorated, and it was likely that coloring was not possible due to oxidative decomposition of the coloring active ingredient. The tattooing paste of comparative example 2 exhibited more micelles, presumably due to inevitable introduction of metal ions during the preparation of the tattooing paste, which may cause micelles and decrease in coloring activity by reacting with genipin. Comparative example 3 using a water-soluble acrylic resin as a dispersant, it was found that a tattoo paste exhibited lumps during storage, resulting in uneven coloring, and that the tattoo paste did not contain an antioxidant, and active ingredients were oxidatively decomposed during storage, resulting in poor coloring effect and short days for maintaining coloring. Compared with the tattooing paste of the comparative example 4 and the comparative example 5, the paste state is not obviously changed, which shows that the ethanol used as a dispersant has better anti-agglomeration effect, and in addition, the disodium ethylene diamine tetraacetate also has certain dispersion, anti-agglomeration and color protection effects, so the tattooing paste has better paste state, the coloring maintenance days are obviously different from the tattooing paste of the example 3, and the compounding of the ethylparaben and the sodium sulfite can play a synergistic antioxidation and anticorrosion role, so that the coloring active ingredient genipin in the tattooing paste of the example 3 is better stored than the tattooing paste of the comparative example 4 and the comparative example 5.

The experiment also proves that the tattooing paste can be stored for more than 6 months at normal temperature through the compounding of the antioxidant and the complexing agent.

Example 15

Tattoo Effect comparison test between tattoo paste of example 3 and a commercially available pigment-based dye paste

The tattooing cream prepared in example 3 and a commercially available pigment tattooing cream were respectively patterned on the skin of an arm, the tattooing effect was observed, the experiment was performed in 7 months in 2018 in western security, the skin applied with the tattooing cream was normally cleaned according to daily living habits after the tattooing cream was dried, no water contact was required, and the experimental results are shown in fig. 2 to 9.

As can be seen from FIG. 2, the tattooing paste according to the present invention is a colorless translucent paste, while the pigment type tattooing paste shows the color of the pigment itself. As can be seen from FIG. 3, the pigment type tattooing paste can be immediately colored after the tattooing paste is dried and removed from the skin surface, but the tattooing paste according to the present invention does not immediately develop color, but can be colored after being cross-linked with the skin, and it is observed that the tattooing paste according to the present invention develops color about 3 hours after being applied. The next day of tattooing, the skin color of the tattooing paste of the present invention appeared dark blue or navy blue, the color was bright and the coloring degree was uniform, and the skin on the applied part of the pigment type tattooing paste began to fade (fig. 4). On the third day of tattooing, the pigment type tattooing cream showed more obvious discoloration, while the tattooing cream of the present invention showed no discoloration (fig. 5). On the fourth day of tattooing, the pigment tattooing paste showed a clear color loss, while the tattooing paste according to the invention showed no color loss (fig. 6). On the fifth day of tattooing, the pigment type tattooing cream products had almost completely faded, while the tattooing pattern of the tattooing cream according to the invention was still clearly visible, with no difference in effect on the second day (fig. 7). FIG. 8 is a schematic diagram showing the effect of the tattooing cream on day 10, and it can be seen that the tattooing pattern of the tattooing cream of the present invention is clearly visible without significant fading. Fig. 9 is a schematic view showing the effect of the tattooing paste on day 20, and it can be seen that the fading of the tattoo pattern begins to appear visually, but the pattern is still clearly visible, which shows that the coloring effect of the tattooing paste according to the present invention can be preserved for more than 20 days.

The above results show that the tattooing paste according to the present invention exhibits superior coloring stability and firmness compared to pigment-based tattooing pastes, and the tattooing effect can be maintained for more than 20 days in summer. The pigment is dyed by covering the pigment with the color of the pigment, so that the dyeing durability and the water resistance are poor, although part of the pigment is reacted to generate a colored substance such as a common protein dyeing reagent, the bonding force of the pigment and protein is weak, and the pigment is easy to fade mainly due to intermolecular acting force, and the coloring of the invention is a final color product formed by the cross-linking reaction of a coloring active substance genipin or geniposidic acid and protein or amino acid, and has the advantages of stable and durable color, no washing-off, low cytotoxicity, good human body compatibility and the like.

The tattooing effect of the tattooing paste can naturally fade along with the metabolism of the skin, the tattoo can be conveniently removed or the tattoo pattern can be conveniently replaced in a short time, the fresh feeling of the tattoo is increased, the duration time of the tattooing effect is related to the metabolism speed of the human body, and the tattooing maintenance time has certain difference for different human bodies. Generally, metabolism in summer is higher than that in winter, so that the tattooing effect can be maintained for more than 30 days in winter. In addition, when tattooing on skin with slower metabolism, such as on the nails, the tattooing effect can be maintained for a longer time.

In addition, the tattooing cream according to the present invention may further include a moisturizing agent having a moisturizing effect, such as glycerin, a lubricant, such as vegetable oil, a fragrance component, such as a natural perfume, which does not affect human skin, and the like.

When the tattooing paste is used, the tattooing paste only needs to be coated on the surface of skin, and after the tattooing paste is dried, the dried tattooing paste is removed, and the tattooing paste can develop color within 3.0-6.0 h generally, and can reach a stable color development state after 12 h. Preferably, after the tattoo paste is removed, the color development can be accelerated by a heat treatment mode, wherein the heat treatment mode comprises hot compress, hot air blowing or light irradiation treatment and the like on the skin to be tattooed, and the heat treatment temperature is 40-50 ℃. Through heat treatment, the crosslinking reaction of genipin or geniposide acid and a nitrogen-containing compound can be accelerated, so that the color development of the tattoo pattern is accelerated and can be realized within 1.5-3.0 h.

The tattooing paste can be coated on the exposed skin of the hollow bottom plate in a coating mode, or the tattooing paste is printed, transferred or sprayed on the skin surface by a tool with patterns, such as a stamp or a jet printing type digital jet printing device, so that blue and/or red tattoos with specific patterns can be formed on the skin surface.

The foregoing is directed to the preferred embodiment of the present invention and is not intended to be exhaustive or to limit the invention to the precise form disclosed. The above-described embodiments are not intended to limit the present invention, and various modifications and variations may occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A tattooing paste comprises 1.0-10.0% of genipin or geniposidic acid, 0.2-2.0% of antioxidant, 1.0-5.0% of thickening agent, 5.0-10.0% of dispersing agent and 72.0-97.2% of water, and is characterized by further comprising 0.1-1.0% of complexing agent.

2. The tattoo paste according to claim 1, wherein the complexing agent is ethylenediaminetetraacetic acid and/or disodium ethylenediaminetetraacetate.

3. The tattooing paste according to claim 1, wherein the antioxidant agent is composed of 0.1-1.0% of water-soluble antioxidant agent and 0.1-1.0% of alcohol-soluble antioxidant agent.

4. The tattooing paste according to claim 2, wherein the water-soluble antioxidant is sodium sulfite or sodium metabisulfite, and the alcohol-soluble antioxidant is methylparaben, ethylparaben, or propylparaben.

5. The tattoo cream according to claim 1, wherein the thickener is any one or more of xanthan gum, sodium carboxymethylcellulose, gum, guar gum, konjac gum, arabinogalactan, pectin, and pullulan.

6. The tattoo paste according to claim 1, wherein the dispersant is ethanol.

7. The preparation method of the tattooing paste is characterized by comprising the following steps:

8. The method of claim 7, wherein the complexing agent is ethylenediaminetetraacetic acid and/or disodium ethylenediaminetetraacetate.

9. The method of claim 7, wherein the water soluble antioxidant is sodium sulfite or sodium metabisulfite and the alcohol soluble antioxidant is methylparaben, ethylparaben, or propylparaben.

10. The method of claim 7, wherein the dispersant is ethanol.

11. A tattoo kit, wherein said tattoo kit comprises:

1) the tattoo paste according to any one of claims 1-6, which is packaged in dark plastic soft bottles with needles;