CN111012686B - Tattooing cream and preparation method and application thereof - Google Patents

Abstract

The invention belongs to the technical field of tattoo dyes, and particularly discloses a tattoo paste and a preparation method and application thereof. The tattooing paste comprises 1.0-10.0% of genipin or geniposide acid, 0.2-2.0% of antioxidant, 1.0-5.0% of thickening agent, 5.0-10.0% of dispersing agent, 0.1-1.0% of complexing agent and 72.0-97.2% of water, wherein the complexing agent is ethylene diamine tetraacetic acid and/or disodium ethylene diamine tetraacetate. The tattooing paste disclosed by the invention avoids the influence of metal ions or non-metal ions on the texture and the coloring activity of the tattooing paste by adding the complexing agent, and effectively ensures the quality of the tattooing paste. In addition, the genipin or geniposide acid is compounded with the thickening agent, the antioxidant, the complexing agent and the dispersing agent to prepare the paste with poor fluidity, which is convenient for operation and implementation, so that the operation and implementation of tattooing are facilitated, and the quality guarantee period of the tattooing paste is effectively prolonged.

Description

Tattooing cream and preparation method and application thereof

Technical Field

The invention belongs to the technical field of tattoo dyes, and particularly relates to a tattoo paste and a preparation method and application thereof.

Background

Tattoos have been part of human culture for over two thousand years, and recently, as society develops, tattoos are becoming popular among young people. The traditional tattooing method is to inject tattooing fluid into the dermal skin layer by a needle or similar tool to make the dye adhere to the skin and display colored patterns, on one hand, the tattooing method needs professional personnel to operate in clean environment or by using sterile equipment, which is expensive, and the tattooing process is often accompanied with skin injury, which may cause infection or cause unrecoverable skin injury; on the other hand, after the tattoo is finished by the method, the tattoo image is difficult to change or remove, and the tattoo is usually removed by a laser or an operation method, which causes secondary damage to the skin of the human body.

In recent years, there is a trend of forming a temporary tattoo pattern capable of maintaining a certain time of coloration on the skin surface by coating the human skin with a tattoo liquid such as impatiens balsamina or others having a coloring effect. Chinese patent application publication No. CN1537900A discloses an ink suitable for tattooing, which is composed of a colorant, a resin, a surfactant, a thickener and a solvent, but the ink forms a colored pattern only by attaching a pigment to the skin surface, has disadvantages of poor water resistance, poor attachment fastness and short durability of tattooing effect, and the pigment constituting the ink may contain heavy metal ions or aromatic amines, etc., which may cause uncomfortable symptoms such as skin allergy, itching, rash, pain, etc., and a method of preparing and using the same. Chinese patent publication No. CN101781530A discloses a self-adhesive tattoo sticker and a method for preparing the same, wherein 0.1-50% of genipin or genipin acid is mixed with 50-99.9% of an adhesive, and the mixture is coated on the surface of a support, and then cut to obtain a sticker with a specific shape, when in use, the sticker is attached to the surface of the skin, and the tattoo effect is achieved by reacting the coloring component genipin or genipin acid in the mixture with an amino compound on the surface of the skin, however, the sticker also has a certain defect, firstly, during the preparation process of the sticker, a genipin or genipin acid sample is directly mixed with the sticker, and as genipin or genipin acid can be dissolved in organic solvents such as methanol and ethanol, the direct mixing with the sticker is difficult to make the sticker or the sticker be mixed with the sticker uniform, resulting in poor coloring effect or non-uniform coloring degree; secondly, the processes of coating genipin or a genipin acid-adhesive mixture, cutting a sticker and the like need specific equipment, and the process is complex; thirdly, the mixture is easily polluted in the coating process of the genipin or the geniposide-adhesive mixture or the cutting and storing process of the paster loaded with the mixture, or the mixture is dried or the coloring component is oxidized and decomposed due to improper storage of the paster, because genipin and geniposide are mostly plant-extracted active substances, the mixture is sensitive to illumination and temperature and is easily affected by the external environment to lose activity, so that the tattooing effect of the self-adhesive tattooing paster is poor, and even the color cannot be developed.

Disclosure of Invention

In view of the above-mentioned deficiencies of the prior art, the present invention aims to provide a tattooing paste with uniform coloring effect, strong durability and clear pattern.

As a first aspect of the present invention, the present invention provides a tattooing paste.

Preferably, the tattooing cream consists of 1.0% genipin, 0.1% sodium sulfite, 0.1% ethylparaben, 0.1% disodium edetate, 1.0% xanthan gum, 5.0% ethanol and 92.7% water.

As a second aspect of the present invention, the present invention provides a method for preparing a tattooing paste.

Preferably, the tattooing cream consists of 1.0% genipin, 0.1% sodium sulfite, 0.1% ethylparaben, 0.1% disodium edetate, 1.0% xanthan gum, 5.0% ethanol and 92.7% water.

Preferably, the preparation of the tattooing paste comprises the following steps:

1) Dissolving genipin and ethylparaben in a dispersing agent;

2) Dissolving sodium sulfite and disodium ethylene diamine tetraacetate into water solution;

3) Dispersing xanthan gum in the solution obtained in the step 1) to obtain a mixed solution;

4) Adding the aqueous solution obtained in the step 2) into the mixed solution obtained in the step 3), and uniformly stirring to obtain the tattoo paste.

As a third aspect of the invention, the invention provides a tattoo kit.

Preferably, the tattoo kit comprises:

1) The tattooing paste consists of 1.0% of genipin, 0.1% of sodium sulfite, 0.1% of ethylparaben, 0.1% of disodium ethylene diamine tetraacetate, 1.0% of xanthan gum, 5.0% of ethanol and 92.7% of water, and is packaged in a dark plastic soft bottle with a needle head;

2) The PVC sticker, the PVC sticker includes adhesion layer and slip sheet, the last fretwork pattern that has of adhesion layer, the thickness on adhesion layer is 0.1~ 0.3mm.

Preferably, the use method of the tattoo set comprises the following steps:

1) Adhering an adhesive layer of the PVC paster to the surface of the skin, wherein the adhesive layer forms a cavity with the thickness of 0.1-0.3 mm on the surface of the skin corresponding to the hollowed-out pattern;

2) Smearing the tattooing ointment on the exposed skin in the cavity, removing the tattooing ointment after the tattooing ointment is dried, and coloring the skin.

Preferably, after removing the tattooing paste, the color development can be accelerated by a heat treatment method, wherein the heat treatment method comprises hot compress, hot air blowing or light irradiation treatment on the skin to be tattooed, and the heat treatment temperature is 40-50 ℃.

Advantageous effects

(1) The tattooing paste disclosed by the invention avoids the influence of metal ions or non-metal ions on the texture and the coloring activity of the tattooing paste by adding the complexing agent, so that the quality and the tattooing effect of the tattooing paste are effectively ensured, and the prepared tattooing paste is safe and reliable.

(2) Genipin is unstable, the solution of genipin is easy to decompose and oxidize, the genipin can be oxidized and decomposed after being placed for about 1 week at room temperature, and the genipin serving as a coloring agent is not easy to control.

(3) The tattooing paste can play a remarkable role in protecting genipin by compounding the water-soluble antioxidant sodium sulfite and the alcohol-soluble grease antioxidant ethylparaben, not only ensures the coloring activity of the tattooing paste, but also effectively prolongs the storage period of the tattooing paste, and can be stored for more than 6 months at normal temperature and more than 12 months at low temperature.

(4) When the tattoo paste is prepared, ethanol is used as a dispersing agent, on one hand, the thickening agent is firstly dispersed in an ethanol solution, and then the aqueous solution is added into the ethanol solution dispersed with the thickening agent, so that the problem that the thickening agent can form a large amount of micelles because the thickening agent is directly dissolved in water in the traditional method is effectively solved, the tattoo paste prepared by the method is uniform in quality and free of micelles, on the other hand, the ethanol is used as a solvent, the dissolubility of genipin can be improved, the skin cuticle can be softened, the genipin can be favorably contacted with the skin, and the color development time is shortened and the coloring effect is enhanced.

(5) The tattoo paste has the advantages of uniform texture, gorgeous and uniform coloring, tattoo effect which can be kept for a long time of 20-30 days, stable and durable coloring, no irritation, good compatibility with human bodies and the like, and the addition amount of genipin forming the tattoo paste is in the range of 1.0-10.0 percent, which is obviously lower than that of the existing tattoo paste, thereby saving the cost.

(6) The invention provides a tattoo suit, wherein a PVC sticker in the tattoo suit has a certain thickness, so that a cavity with a specific shape can be formed when the PVC sticker is pasted on the surface of the skin, the cavity is used for containing tattoo liquid on one hand, and the other side is used for indicating the coating thickness of the tattoo liquid on the other hand. The invention can ensure that the effective components of the tattooing cream are not influenced by subpackaging the tattooing cream and the PVC paster, ensure the quality and the tattooing effect of the tattooing cream, and facilitate the circulation and the transportation of the tattooing cream by adopting the tattooing suit of the invention.

(7) The tattoo cream is prepared from common raw materials in cosmetics, the addition amount is controlled within a range acceptable by human bodies, no damage is caused to the skin, no obvious skin symptoms such as erythema or wheal and the like appear in the using process, and the tattoo cream has the advantages of safety, reliability and good general applicability.

Drawings

FIG. 1 is a diagram of a possible reaction mechanism of genipin and nitrogen-containing compounds.

FIG. 2 is a schematic diagram showing the coating effect of the tattooing paste according to the present invention and commercially available pigment-based tattooing pastes.

FIG. 3 is a schematic diagram showing the effects of the tattooing paste of the present invention and commercially available pigment-based tattooing pastes after coating.

FIG. 4 is a schematic diagram showing the effect of tattooing the tattooing cream of the present invention and commercially available pigment-based tattooing cream on day 2.

FIG. 5 is a schematic diagram showing the effect of tattooing on day 3 of the tattooing paste of the present invention and commercially available pigment-based tattooing paste.

FIG. 6 is a schematic diagram showing the effect of tattooing on day 4 of the tattooing paste of the present invention and commercially available pigment-based tattooing paste.

FIG. 7 is a schematic diagram showing the effect of tattooing on day 5 of the tattooing paste of the present invention and commercially available pigment-based tattooing paste.

FIG. 8 is a schematic view showing the effect of tattooing the tattooing cream according to the present invention on day 10.

FIG. 9 is a schematic view showing the tattooing effect of the tattooing paste according to the present invention on day 20.

Detailed Description

Genipin

Genipin (Genipin, C) ₁₁ H ₁₄ O ₅ ) The eucommia ulmoides oliv extract is one of active ingredients of the traditional Chinese medicine eucommia ulmoides, is a natural product obtained by hydrolyzing Geniposide (Geniposide) with beta-glucosidase, belongs to iridoid heterocyclic compounds, has multiple physiological functions of liver protection, gallbladder benefiting, anti-inflammatory action, antithrombotic action, blood sugar lowering action, anti-tumor action and the like, is commonly used for preparing medicines for treating liver diseases, blood pressure lowering and diabetes, is used for preparing various biological auxiliary materials as a biological cross-linking agent, is a white crystal, is soluble in water and organic solvents such as methanol, ethanol and the like, and mainly exists in natural plants such as gardenia, eucommia ulmoides, and fruits of genipa americana. Genipin has very active chemical property, and when the genipin is combined with compounds with amino groups (such as amino acid, collagen, chitosan, glucamine compounds and various proteins and enzymes), the genipin reacts to form a safe, reliable and non-toxic natural pigment, namely gardenia blue pigment, which has a blue color and a possible reaction mechanism that: genipin is firstly attacked by amino nucleophilic gene of nitrogen-containing compound to generate ring opening, and reacts with nitrogen-containing compound to generate a nitride of iridoid, i.e. a compound of genipin and nitrogen-containing compound is formed, and then the compound is dehydrated to form a compoundAromatic monomers, which may then form cyclic intermolecular and intramolecular cross-linked structures due to dimerization polymerization based on a radical reaction, to obtain gardenia blue pigment cross-linked products (fig. 1), and thus genipin is often used as a fingerprint color developer in the medical or detection fields. In addition, because genipin is typically derived from plant materials, it offers great potential for using genipin-derived colorants in bread and canned food applications.

The gardenia blue pigment and the gardenia red pigment have stronger coloring capability and stability, can keep the relative stability of color generation in a larger pH range, and are also relatively stable to heat and light. The invention utilizes genipin to generate cross-linking reaction with nitrogen-containing compounds on the surface of the skin, such as protein, and generates cross-linked gardenia blue pigment on the surface layer of the skin, thereby realizing the tattooing effect. The gardenia blue pigment is an intermolecular and intramolecular crosslinked structure formed by genipin and nitrogen-containing substances on the surface layer of the skin, so that the gardenia blue pigment has strong coloring stability and coloring durability.

The genipin used in the present invention may be prepared by extraction from natural plants or by chemical synthesis methods known in the art, and preferably, the genipin used is derived from plant extracts.

Complexing agents

Metal ions, even heavy metal ions such as mercury, lead, cadmium and the like, may be inevitably introduced during the preparation and storage of the tattoo paste, for example, metal ions may be contained in the raw materials constituting the tattoo paste, or metal ions may be introduced during the equipment and operation processes used in the preparation process, and these metal ions may react with the active substance genipin, resulting in the reduction of the coloring activity of genipin and the agglomeration or precipitation of the tattoo paste during the storage process. In addition, if heavy metal ions exist in the tattooing paste, the safety of the product cannot be guaranteed. The complexing agent can effectively remove metal ions and heavy metal ions, can be used for forming a stable system, prevents the tattoo paste from flocculating, agglomerating and discoloring, and reduces coloring difference.

Disodium ethylene diamine tetraacetate is a commonly used excellent complexing agent, has a high stability constant, can generate a stable complex with most metal ions except alkali metal, eliminates the metal ions or harmful effects caused by the metal ions, can also play roles of oxidation resistance, corrosion resistance, color protection and dispersion, and is beneficial to protecting genipin.

Thickening agent

The thickening agent is added into the tattooing paste, so that on one hand, the viscosity of the tattooing paste is adjusted, the genipin and the thickening agent are prepared into the paste with poor fluidity, the tattooing paste can be easily formed into a film on the surface of the skin, the adhesive force between the tattooing paste and the skin is increased, and the tattooing operation and the preservation of the tattooing paste are facilitated. In addition, the thickening agent also has dispersing and stabilizing effects, and is beneficial to preventing flocculation or sedimentation of the tattoo paste during storage.

The thickener is preferably added in an amount of 1.0 to 2.0% because the viscosity of the tattooing paste can be improved, but if the viscosity of the tattooing paste is too high, it is difficult to dry or not dry naturally on the skin surface, which may affect the manipulation of tattooing.

Various types of thickeners commonly used in the art are suitable for use in the present invention, but it is desirable that the thickener not be a compound having a primary amine group that is capable of reacting with genipin, such as chitosan, gelatin, and the like. Thickeners that may be used include xanthan gum, sodium carboxymethylcellulose, gums, guar gum, konjac gum, arabinogalactans, pectins, pullulans, and the like. The xanthan gum is a biological gum with excellent performance, has the characteristics of low concentration and high viscosity, and has very good stability for acid-base and enzymolysis, and the sodium carboxymethyl cellulose is used as a cellulose thickening agent to easily form a transparent colloidal solution. The thickening agent is often applied to the food field and the cosmetic field for improving the performance of food or cosmetics, has high safety, has no irritation when being applied to the tattooing cream, and does not cause discomfort and allergy symptoms such as pruritus, pain and the like.

Antioxidant agent

Genipin is unstable, and the solution of genipin is easy to be oxidized and decomposed at room temperature for 1 week, so that the coloring activity is lost. Sodium sulfite is a commonly used water-soluble antioxidant and has significant antioxidant and preservative effects. The ethylparaben is a common grease antioxidant and preservative, has wide antibacterial effects on mould, yeast and bacteria, has the characteristics of strong antibacterial ability, low toxicity and no irritation, and is widely applied to cosmetics. The invention discovers that the combination of the water-soluble antioxidant and the alcohol-soluble grease antioxidant can obviously inhibit the oxidative decomposition of genipin in the tattooing paste, ensure the coloring activity of the tattooing paste and effectively prolong the storage period of the tattooing paste.

Dispersing agent

The purpose of adding the dispersant to the tattoo cream of the present invention is to improve coloring ability and coloring uniformity and prevent the tattoo cream from flocculating and settling. The dispersant classes include fatty acids, fatty amides, and esters, although not any dispersant is suitable for use in the present invention. First, the dispersant cannot be a compound having a primary amine group that can react with genipin.

The invention finds that ethanol is used as a dispersing agent to have good dispersing effect, and when the ethanol is used as the dispersing agent, other dispersing agents are not required to be added, so that the obtained tattooing paste has good performance. The inventor finds that a large amount of micelles can be formed if the thickening agent is directly added into water in the preparation process of the tattoo paste, the thickening agent cannot be completely dissolved, and the tattoo paste is very unfavorable for preparing the tattoo paste. The thickening agents used in the present invention are in the form of powders such as xanthan gum and sodium carboxymethylcellulose, which are extremely hydrophilic, and if added directly to water, the outer layer absorbs water and swells into a micelle, preventing water from entering the inner layer, which reduces its solubility in water. In the present invention, when the thickener is added to ethanol, since the thickener is insoluble in ethanol and is dispersed in ethanol to form individual fine particles, at this time, no micelle is formed by adding an aqueous solution, and thus the tattooing cream prepared is uniform in texture without agglomeration.

On the other hand, ethanol is also used as a solvent in the present invention for improving the solubility of genipin and for dissolving alcohol-soluble substances. On the other hand, ethanol can soften the stratum corneum of the skin, which is beneficial for the contact of the coloring active substance in the tattoo paste with the skin, thereby being beneficial to enhancing the coloring effect and shortening the color development time. In the present invention, the ethanol used is absolute ethanol.

Detailed Description

The present invention will be described in more detail with reference to examples. It is to be understood that the practice of the invention is not limited to the following examples, and that any variations and/or modifications may be made thereto without departing from the scope of the invention.

In the invention, all parts and percentages are weight units, and all equipment, raw materials and the like can be purchased from the market or are commonly used in the industry, if not specified. Unless otherwise indicated, the examples employ methods that are within the ordinary skill in the art. In the present invention, genipin was purchased from shin-biology, linchuan, and the pigment tattooing cream used was a commercially available Haina tattooing cream.

Example 1

A tattoo paste is prepared by the following steps:

dissolving 1.0% genipin and 0.1% ethylparaben in 5.0% ethanol to obtain an alcoholic solution, dissolving 0.1% sodium sulfite and 0.1% disodium edetate in 92.7% water to obtain an aqueous solution, dispersing 1.0% xanthan gum in the alcoholic solution, slowly adding the aqueous solution into the alcoholic solution in which the xanthan gum is dispersed, and uniformly stirring to obtain the tattooing paste.

Example 2

In order to achieve convenient and simple operation of tattooing, the present embodiment provides a tattooing set, which comprises the following components:

(1) Tattooing paste: the tattooing paste comprises 1.0% of genipin, 1.0% of xanthan gum, 0.1% of sodium sulfite, 0.1% of ethylparaben, 0.1% of disodium ethylene diamine tetraacetate, 5% of ethanol and 92.7% of water; the preparation method of the tattooing paste is as described in example 1, and the tattooing paste is packaged in dark plastic soft bottles with needles;

(2) PVC paster: the PVC sticker includes adhesion layer and slip sheet, the last fretwork pattern that has of adhesion layer, the thickness on adhesion layer is 0.1mm.

In the embodiment, the tattooing paste is separately filled in a dark plastic soft bottle with a needle head for the purpose of convenient storage and transportation, on one hand, the tattooing paste is in a sealed and light-proof storage state before use, and all components are not influenced by the external storage environment, especially, genipin is ensured not to be oxidized and decomposed or irradiated and decomposed, and the good quality and tattooing effect of the tattooing paste can be ensured; on the other hand, when the tattooing paste is used, the tattooing paste can be released from the needle head by extruding the plastic soft bottle, so that the operation is convenient. Any dark coloured plastic soft bottle with a needle, such as brown, brown or black soft bottles, commonly used in the art, can be used in the present invention, a preferred form being shown in appearance patent CN 201730454811.3.

In the embodiment, the PVC sticker is a form commonly used in the field, and the adhesion layer of the PVC sticker is provided with an adhesive and can be adhered to the surface of the skin; the PVC sticker has a specific hollowed-out pattern on an adhesive layer, and the pattern is prepared by a cutting machine or a carving machine; the lining paper and the adhesive layer are adhered together to protect the adhesive on the adhesive layer, and when the tattoo adhesive is used, the lining paper is removed, and the adhesive layer is adhered to the surface of the skin needing tattoo. When the adhesion layer of the PVC sticker is stuck on the surface of the skin, the skin at the hollow pattern is exposed, and at the moment, the adhesion layer of the PVC sticker has a certain thickness, so that a cavity with a specific pattern is formed on the skin by the adhesion layer, and the tattooing can be realized by smearing the tattooing paste on the exposed skin in the cavity. For general consumers, it is not clear how much the tattoo paste should be applied properly, and the amount of the tattoo paste should be too small, which results in light color, too much amount of the tattoo paste and unnecessary waste, and the cavity formed on the skin surface by the PVC sticker of the present invention is used for accommodating the tattoo paste on one hand, and for indicating the amount of the tattoo paste to avoid too much or too little amount of the tattoo paste on the other hand, the inventor has found through experiments that when the thickness of the adhesive layer of the PVC sticker is 0.1-0.3 mm, i.e. the height of the cavity with a specific pattern formed on the skin from the skin surface is 0.1-0.3 mm, the cavity is filled with the tattoo paste, and the obtained tattoo effect is optimal. This provides convenient guidance to the consumer in controlling the amount of tattoo paste applied to achieve the best tattoo results.

The use method of the tattoo suit of the embodiment comprises the following steps: removing the lining paper from the PVC sticker, attaching the adhesive layer to the clean skin surface, smearing the tattooing paste in a cavity formed by the adhesive layer and the skin surface, and removing the adhesive layer and the tattooing paste from the skin surface after the tattooing paste is dried on the skin surface (about 10-30 min), wherein the skin shows a black-blue pattern after 3 hours.

The use method of the invention is convenient to operate, the consumer can realize tattooing without professional personnel, and the subpackaging of the tattooing paste and the PVC sticker can ensure that the effective components of the tattooing paste are not affected and the tattooing effect of the tattooing paste is ensured.

Comparative example 1

A tattoo paste is prepared by the following steps:

mixing 10.0% genipin and 90.0% non-setting adhesive glue, and stirring to obtain tattooing paste.

Comparative example 2

A tattoo paste is prepared by the following steps:

dissolving 1.0% genipin and 0.1% ethylparaben in 5.0% ethanol to obtain an alcoholic solution, dissolving 0.1% sodium sulfite in 92.8% water to obtain an aqueous solution, dispersing 1.0% xanthan gum in the alcoholic solution, slowly adding the aqueous solution into the alcoholic solution in which the xanthan gum is dispersed, and uniformly stirring to obtain the tattooing paste.

Comparative example 3

A tattooing paste, the preparation method comprises the following steps:

dissolving 1.0% genipin, 5.0% water-soluble acrylic resin and 0.1% disodium ethylene diamine tetraacetate in 92.9% water to obtain an aqueous solution, slowly dissolving 1.0% xanthan gum in the aqueous solution, and uniformly stirring to obtain the tattoo paste.

Comparative example 4

A tattoo paste is prepared by the following steps:

dissolving 1.0% genipin and 0.1% ethylparaben in 5.0% ethanol to obtain an alcoholic solution, dissolving 0.1% disodium edetate in 92.8% water to obtain an aqueous solution, dispersing 1.0% xanthan gum in the alcoholic solution, slowly adding the aqueous solution into the alcoholic solution in which the xanthan gum is dispersed, and uniformly stirring to obtain the tattooing paste.

Comparative example 5

A tattooing paste, the preparation method comprises the following steps:

dissolving 1.0% genipin in 5.0% ethanol to obtain an alcohol solution, dissolving 0.1% sodium sulfite and 0.1% disodium ethylene diamine tetraacetate in 92.8% water to obtain an aqueous solution, dispersing 1.0% xanthan gum in the alcohol solution, slowly adding the aqueous solution into the alcohol solution in which the xanthan gum is dispersed, and uniformly stirring to obtain the tattooing paste.

Example 3

Sensitivity test of the tattooing cream prepared in example 1.

The tattooing cream of example 1 was tested by applying trial, 14 healthy subjects aged 25 to 40 were selected and randomly divided into 7 groups, and two persons were selected, and all subjects had healthy and smooth arm skin, no rash such as erythema and desquamation, and no other skin diseases or systemic diseases. Before the test, each subject signs an informed consent, after the skin is cleaned, the tattoo cream prepared in the example 1 is respectively smeared on the skin of the arm, the skin is observed once per hour, whether erythema and edema are formed is determined, and no erythema and edema appear on the skin surface of all the subjects within 12 hours.

Example 4

Test for coloring effect and stability of tattooing pastes prepared in example 1 and comparative examples 1-5.

33 SD rats of 10 weeks old cleaning grade are purchased from the animal experiment center of Shanxi Chinese medicine university, the male and female are unlimited and randomly divided into 11 groups of 3 rats, and 2 multiplied by 2cm is selected from the backs of the rats ² The area (2) is removed of body hair, and the epidermis is not damaged by attention, so that the skin is completely leaked. The tattooing ointments prepared in the above examples and comparative examples were applied to the skin on the outer leakage, respectively, and fed with a common feed in a single cage, the feeding temperature (20 ℃. + -. 2 ℃) and the humidity 60%. + -. 5%, the skin color change was recorded every day until the coloration completely disappeared, and the average value was calculated for the days for maintaining the coloration, and the results are shown in the following table:

TABLE 1 tattoo cream coloring stability test results

Group of	Coloring effect	Days of maintenance of staining
			Example 1	Showing dark blue color, uniform coloring and bright color	26
Comparative example 1	Lighter and uneven coloration	5
			Comparative example 2	Showing dark blue color, uniform coloring and bright color	24
Comparative example 3	Showing a bluish color and non-uniform coloring	20
			Comparative example 4	Showing dark blue color, uniform coloring and bright color	21
Comparative example 5	Is black blue, is uniformly colored and has bright color	20

As can be seen from the above results, the tattooing paste according to the present invention is colored uniformly, and is bright in color, and the tattooing effect and the coloring stability are significantly higher than those of the comparative examples. The comparative example 1 is prepared by directly mixing a genipin sample and non-setting adhesive glue, the tattooing paste prepared by the method has uneven texture, and the genipin can not be completely dissolved in the non-setting adhesive glue to obtain the paste, so the coloring effect is poor, and the coloring maintenance days are short. Comparative example 3 using a water-soluble acrylic resin as a dispersant, it was found that the effect of dispersion was inferior to that of the tattooing paste according to the present invention, resulting in uneven coloring. The tattooing pastes of comparative examples 2-5 did not show significant difference in coloring stability from example 1.

Example 5

Stability test experiment of the tattooing pastes prepared in example 1 and comparative examples 1-5.

After the tattooing pastes prepared in example 1 and comparative examples 1-5 were stored at normal temperature for 6 months, the surface state of the tattooing paste was observed, and the coloring effect of the tattooing paste after being left for 6 months was measured by the method described in example 4, and the experimental results are shown in the following table:

TABLE 2 tattoo cream stability test results

From the above results, it can be seen that the surface condition of the tattooing paste according to the present invention does not change significantly after 6 months of storage at room temperature, and still has significant coloring effect, whereas the tattooing paste according to comparative example 1 has significantly changed texture, darkened color of the paste, and a large amount of micelles appeared, and it was found that coloring was not possible after application, indicating that the tattooing paste was deteriorated, and it was likely that coloring was not possible due to oxidative decomposition of the coloring active ingredient. The tattooing paste according to comparative example 2 exhibited more micelles, presumably due to inevitable introduction of metal ions during the preparation of the tattooing paste, which may cause the appearance of micelles and a decrease in coloring activity by reacting with genipin. Comparative example 3 using a water-soluble acrylic resin as a dispersant, it was found that the tattooing paste exhibited lumps during storage, resulting in uneven coloring, and that the tattooing paste did not contain an antioxidant, and the active ingredients were oxidatively decomposed during storage, resulting in poor coloring effect and a short number of days for maintaining coloring. Compared with the tattooing paste of the comparative example 4 and the comparative example 5, the paste state is not obviously changed, which shows that the ethanol used as a dispersant has better anti-agglomeration effect, and in addition, the disodium ethylene diamine tetraacetate also has certain dispersion, anti-agglomeration and color protection effects, so the tattooing paste has better paste state, the coloring maintenance days are obviously different from the tattooing paste of the example 1, and the compounding of the ethylparaben and the sodium sulfite can play a synergistic antioxidation and anticorrosion role, so that the coloring active ingredient genipin in the tattooing paste of the example 1 is better stored than the tattooing paste of the comparative example 4 and the comparative example 5.

The experiment also proves that the tattooing paste can be stored for more than 6 months at normal temperature through the compounding of the antioxidant and the complexing agent.

Example 6

Tattoo Effect comparison test between tattooing paste of example 1 and a commercially available pigment-based coloring paste

The tattooing cream prepared in example 1 and a commercially available pigment tattooing cream were respectively patterned on the skin of an arm, tattooing effect was observed, experiments were carried out in west safety in 2018 and 7 months, the skin coated with the tattooing cream can be normally cleaned according to daily living habits after the tattooing cream is dried without avoiding water contact, and the experimental results are shown in fig. 2 to 9.

As can be seen from FIG. 2, the tattooing paste according to the present invention is a colorless translucent paste, while the pigment type tattooing paste shows the color of the pigment itself. As can be seen from FIG. 3, the pigment type tattooing paste can be immediately colored after the tattooing paste is dried and removed from the skin surface, but the tattooing paste according to the present invention does not immediately develop color, but can be colored after being cross-linked with the skin, and it is observed that the tattooing paste according to the present invention develops color about 3 hours after being applied. The next day of tattooing, the skin color of the tattooing paste of the present invention appeared dark blue or navy blue, the color was bright and the coloring degree was uniform, and the skin on the applied part of the pigment type tattooing paste began to fade (fig. 4). On the third day of tattooing, the pigment type tattooing cream showed more obvious discoloration, while the tattooing cream of the present invention showed no discoloration (fig. 5). On the fourth day of tattooing, the pigment tattooing paste showed a clear color loss, while the tattooing paste according to the invention showed no color loss (fig. 6). On the fifth day of tattooing, the pigment tattooing paste products almost completely faded, while the tattooing pattern of the tattooing paste according to the invention remains clearly visible, with no difference in effect on the second day (fig. 7). FIG. 8 is a schematic diagram showing the effect of the tattooing cream on day 10, and it can be seen that the tattooing pattern of the tattooing cream of the present invention is clearly visible without significant fading. Fig. 9 is a schematic view showing the effect of the tattooing paste on day 20, and it can be seen that the fading of the tattoo pattern begins to appear visually, but the pattern is still clearly visible, which shows that the coloring effect of the tattooing paste according to the present invention can be preserved for more than 20 days.

The above results show that the tattooing paste according to the present invention exhibits superior coloring stability and firmness compared to pigment-based tattooing pastes, and the tattooing effect can be maintained for more than 20 days in summer. The pigment is dyed by covering the pigment with the color of the pigment, so that the dyeing durability and the water resistance are poor, although part of the pigment is also reacted to generate a colored substance such as a common protein dyeing reagent, the bonding force of the pigment and protein is weak, and the pigment is easy to fade mainly due to intermolecular acting force, and the coloring of the invention is a final color product formed by the cross-linking reaction of a coloring active substance genipin and protein or amino acid, and has the advantages of stable and durable color, no washing-off, low cytotoxicity, good human body compatibility and the like.

The tattooing effect of the tattooing paste can naturally fade along with the metabolism of the skin, the tattoo can be conveniently removed or the tattoo pattern can be conveniently replaced in a short time, the fresh feeling of the tattoo is increased, the duration time of the tattooing effect is related to the metabolism speed of the human body, and the tattooing maintenance time has certain difference for different human bodies. Generally, metabolism in summer is higher than that in winter, so that the tattooing effect can be maintained for more than 30 days in winter. In addition, when tattooing on skin with slower metabolism, such as on the nails, the tattooing effect can be maintained for a longer time.

In addition, the tattooing cream according to the present invention may further include a moisturizing agent having a moisturizing effect, such as glycerin, a lubricant, such as vegetable oil, a fragrance component, such as a natural perfume, which does not affect human skin, and the like.

When the tattooing paste is used, the tattooing paste only needs to be coated on the surface of skin, the dried tattooing paste is removed after the tattooing paste is dried, the tattooing paste can be developed within 3.0-6.0 h generally, and the tattooing paste can reach a stable color development state after 12 h. Preferably, after removing the tattooing paste, the color development can be accelerated by a heat treatment method including hot compress, hot air blow, light irradiation, etc. on the skin to be tattooed, wherein the heat treatment temperature is 40-50 ℃. Through heat treatment, the crosslinking reaction of genipin and the nitrogen-containing compound can be accelerated, so that the tattoo pattern can be developed in an accelerated manner within 1.5-3.0 h.

The tattooing paste can be coated on the bare skin of the hollow bottom plate in a coating mode, or a tool with patterns, such as a seal or a jet printing type digital jet printing device, is adopted to print, transfer or spray the tattooing paste on the skin surface, so that a blue and/or red tattoo with a specific pattern can be formed on the skin surface.

The foregoing is directed to the preferred embodiment of the present invention and is not intended to be exhaustive or to limit the invention to the precise form disclosed. The above-described embodiments are not intended to limit the present invention, and various modifications and variations may occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (2)

1. A tattooing paste, which is characterized in that the tattooing paste consists of 1.0% genipin, 0.1% sodium sulfite, 0.1% ethylparaben, 0.1% disodium ethylenediamine tetraacetic acid, 1.0% xanthan gum, 5.0% ethanol and 92.7% water, wherein the preparation of the tattooing paste comprises the following steps:

1) Dissolving 1.0% genipin and 0.1% ethylparaben in 5.0% ethanol;

2) Dissolving 0.1% sodium sulfite and 0.1% disodium ethylene diamine tetraacetate in 92.7% water to obtain an aqueous solution;

3) Dispersing 1.0% xanthan gum in the solution obtained in the step 1) to obtain a mixed solution;

4) Adding the aqueous solution obtained in the step 2) into the mixed solution obtained in the step 3), and uniformly stirring to obtain the tattoo paste.

2. A tattoo kit, wherein said tattoo kit comprises:

1) The tattoo paste of claim 1, wherein said tattoo paste is dispensed into dark plastic soft bottles with needles;

2) The PVC sticker, the PVC sticker includes adhesion layer and slip sheet, the last fretwork pattern that has of adhesion layer, the thickness on adhesion layer is 0.1~ 0.3mm.

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