CN111011270B - Method for relieving fish transportation stress and application - Google Patents

Method for relieving fish transportation stress and application Download PDF

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Publication number
CN111011270B
CN111011270B CN201911230775.7A CN201911230775A CN111011270B CN 111011270 B CN111011270 B CN 111011270B CN 201911230775 A CN201911230775 A CN 201911230775A CN 111011270 B CN111011270 B CN 111011270B
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vitamin
fish
stress
transportation
relieving
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CN111011270A (en
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王庆奎
邢克智
程镇燕
赵慧妍
刘桐
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Tianjin Agricultural University
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Tianjin Agricultural University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/10Culture of aquatic animals of fish
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/174Vitamins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Chemical & Material Sciences (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Birds (AREA)
  • Insects & Arthropods (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The invention relates to a method for relieving fish transportation stress, which comprises the following steps: adding 5-20 mg of vitamin B into each liter of transport water22-10 g of vitamin C. The method for transporting the fish is beneficial to maintaining the relative stability of physiological and biochemical indexes of the fish, relieving the adverse effect of transportation stress on the fish, and has low cost and easy realization, thereby having good popularization prospect.

Description

Method for relieving fish transportation stress and application
Technical Field
The invention belongs to the technical field of aquatic animal living body transportation, and particularly relates to a method for relieving fish transportation stress and application.
Background
Living body transportation is a routine management operation in aquaculture management. Crowding and bumping during transportation cause stress to aquatic animals: jump, collision, sudden rise of oxygen consumption rate, increased secretion of mucus on the body surface and abnormal physiological and biochemical indexes, thereby causing body surface injury, water quality deterioration, endocrine disturbance, immunity reduction and even death.
Low temperature transportation has become a commonly used mode of transportation for aquatic products. The low temperature can obviously reduce the metabolic rate and stress reaction of aquatic products, thereby improving the transportation survival rate of the aquatic products. However, transportation stress response to aquatic animals cannot be avoided by low-temperature transportation.
The B vitamins are beneficial to repairing damaged mucous membranes and enhancing the immunity of organisms, and the vitamin C is beneficial to relieving the stress response of animals. Two vitamins are common feed additives.
Through searching, the following two patent publications related to the patent application of the invention are found:
1. a plant extract for reducing fish transportation stress and application thereof (CN105816558A) comprise the following components in parts by weight: 5-15 parts of rheum officinale, 10-20 parts of scutellaria baicalensis, 2-8 parts of phellodendron amurense, 2-8 parts of liquorice, 5-15 parts of sophora flavescens, 5-15 parts of oriental wormwood, 5-15 parts of Sichuan 5, 5-15 parts of coptis chinensis and 2-8 parts of astragalus membranaceus. The plant extract provided by the invention can be applied to transporting tilapia fingerlings, the average oxygen consumption rate and ammonia discharge rate of the plant extract are obviously reduced compared with those of a blank group, the survival rate can be improved, and the plant extract adopts common traditional Chinese medicine raw materials, is stable in source, safe and nontoxic, and has a great application prospect.
2. A live fish transportation stress repairing agent and a preparation method (CN107117665A) thereof are disclosed, the live fish transportation stress repairing agent is composed of the following raw materials in parts by weight: 200 portions of dried ginger powder, 850 portions of anhydrous sodium thiosulfate and 150 portions of zeolite powder. The live fish transportation stress restoration agent provided by the invention has the advantages of low cost, high efficiency, rapidness, effective prevention of ammonia nitrogen pollution in the transportation process, enhancement of fish immunity and improvement of transportation survival rate.
By contrast, the present patent application is substantially different from the above patent publications.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides the method and the application for relieving the fish transportation stress.
The technical scheme adopted by the invention for solving the technical problems is as follows:
a method of mitigating fish transport stress, the method comprising:
adding 5-20 mg of vitamin B into each liter of transport water22-10 g of vitamin C.
Moreover, the method comprises the following steps:
10mg of vitamin B per liter of transport water23.4g vitamin C.
Furthermore, the vitamin B2And the vitamin C is food grade or medicine grade.
The method for relieving fish transportation stress is applied to fish transportation.
The invention has the advantages and positive effects that:
the method for transporting the fish is beneficial to maintaining the relative stability of physiological and biochemical indexes of the fish, relieving the adverse effect of transportation stress on the fish, and has low cost and easy realization, thereby having good popularization prospect.
Detailed Description
The following detailed description of the embodiments of the present invention is provided for the purpose of illustration and not limitation, and should not be construed as limiting the scope of the invention.
The raw materials used in the invention are conventional commercial products unless otherwise specified; the methods used in the present invention are conventional in the art unless otherwise specified.
A method of mitigating fish transport stress, the method comprising:
adding 5-20 mg of vitamin B into each liter of transport water22-10 g of vitamin C.
Preferably, the method comprises the following steps:
10mg of vitamin B per liter of transport water23.4g vitamin C.
Preferably, the vitamin B2And the vitamin C is food grade or medicine grade.
The method for relieving fish transportation stress can be applied to fish transportation.
Preferably, in the transportation process of the fish, in addition to reducing the water temperature, 10mg of vitamin B is added into each liter of water23.4g vitamin C.
Preferably, the vitamin B used2Vitamin C is commercially available, either food grade or pharmaceutical grade, and vitamin C is common L-ascorbic acid.
The relevant verification of the invention is as follows:
1. transport of Fugu rubripes for 30 minutes
Taking out young fish (23.8 + -3.8 g) of Fugu rubripes bred in seawater of 23 deg.C, placing into water tank containing seawater of 17 deg.C, and adding vitamin B210mg/L, 3.4g/L vitamin C, and no vitamin in the control group. The transport density is 10.3 +/-1.7 g/L, the air pump is used for continuously inflating, and the transport lasts for 30 minutes. After the seeds are transported to the destination, the seeds are put into a water tank for domestication. Sampling on days 1, 3, 5 and 7 of domestication, and detecting physiological and biochemical indexes of fish bodies. The results show that the blood leukocytes, lymphocytes, intermediate cells and neutrophils of the takifugu rubripes are soaked with vitamins during transportationCells and platelets were relatively stable, whereas the control group was significantly elevated (p)<0.05); the fish soaked with vitamins, liver and pancreas superoxide dismutase (SOD), Lysozyme (LZM), aspartate Aminotransferase (AST), alanine Aminotransferase (ALT), lactic acid (LD), Catalase (CAT), Malondialdehyde (MDA), and reduced Glutathione (GSH) are relatively stable, while the control group is significantly increased (p)<0.05). The vitamin soaking is helpful for maintaining the relative stability of physiological and biochemical indexes of the takifugu rubripes and relieving the stress on the takifugu rubripes caused by transportation.
TABLE 1 Effect of vitamin soaking on the physiological and biochemical indicators of blood of Fugu rubripes during transportation (30min)
Figure BDA0002303478780000031
Note:*indicating significant difference (p) between the same sampling times<0.05), the same row of data is marked with different letters, which indicates that the difference is significant (p)<0.05)。
TABLE 2 Effect of vitamin soaking on Biochemical indicators of liver and pancreas of Takifugu rubripes during transport (30min)
Figure BDA0002303478780000041
Note:*indicating significant difference (p) between the same sampling times<0.05), different letters in the same row indicate that the same treatment is significantly different (p)<0.05)。
Although the embodiments of the present invention have been disclosed for illustrative purposes, those skilled in the art will appreciate that: various substitutions, changes and modifications are possible without departing from the spirit and scope of the invention and the appended claims, and therefore the scope of the invention is not limited to the embodiments disclosed.

Claims (3)

1. A method of mitigating fish transport stress, comprising: the method comprises the following steps:
adding 5-20 mg vitamin into per liter of transport waterBiotin B22-10 g of vitamin C;
the vitamin B2And the vitamin C is food grade or medicine grade.
2. The method of mitigating fish transport stress of claim 1, wherein: the method comprises the following steps: 10mg of vitamin B per liter of transport water23.4g vitamin C.
3. Use of a method for relieving fish transport stress according to claim 1 or 2 in fish transport.
CN201911230775.7A 2019-12-05 2019-12-05 Method for relieving fish transportation stress and application Active CN111011270B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201911230775.7A CN111011270B (en) 2019-12-05 2019-12-05 Method for relieving fish transportation stress and application

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201911230775.7A CN111011270B (en) 2019-12-05 2019-12-05 Method for relieving fish transportation stress and application

Publications (2)

Publication Number Publication Date
CN111011270A CN111011270A (en) 2020-04-17
CN111011270B true CN111011270B (en) 2021-12-07

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006342111A (en) * 2005-06-09 2006-12-21 Marubeni Nisshin Feed Co Ltd Vaccine stress relieving agent
CN109105634A (en) * 2018-07-25 2019-01-01 东阿阿胶股份有限公司 A kind of additive for preventing to alleviate donkey transport stress
CN110447768A (en) * 2019-07-11 2019-11-15 南京农业大学 It is a kind of promoted transportation of live fish survival rate additive and its application

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102406091B (en) * 2011-12-02 2013-03-20 中国水产科学研究院淡水渔业研究中心 Composite preparation for improving fish body transportation stress capability as well as preparation method and application thereof
CN102578014A (en) * 2012-03-14 2012-07-18 中国水产科学研究院淡水渔业研究中心 Method for improving survival rate of rice field eels in transportation process
CN102907364A (en) * 2012-11-14 2013-02-06 中国水产科学研究院淡水渔业研究中心 Method for improving survival rate of triangular bream in long-distance transport
CN102919178A (en) * 2012-11-16 2013-02-13 中国水产科学研究院淡水渔业研究中心 Method for improving survival rate of shrimp seed of south America white shrimp in long-distance transport
CN103931947A (en) * 2014-04-29 2014-07-23 临湘市东昇生物科技有限公司 Vitaminum c (Vc)-containing fish medicine
CN104431640A (en) * 2014-12-31 2015-03-25 通威股份有限公司 Silver salmon anti-stress fodder
KR20170125209A (en) * 2016-05-04 2017-11-14 강원대학교산학협력단 Feeding method of poultry feed comprising betaine with vitamin C cold water

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006342111A (en) * 2005-06-09 2006-12-21 Marubeni Nisshin Feed Co Ltd Vaccine stress relieving agent
CN109105634A (en) * 2018-07-25 2019-01-01 东阿阿胶股份有限公司 A kind of additive for preventing to alleviate donkey transport stress
CN110447768A (en) * 2019-07-11 2019-11-15 南京农业大学 It is a kind of promoted transportation of live fish survival rate additive and its application

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