CN110984092A - On-site test method for building ecological irrigation canal based on microbial mineralization - Google Patents
On-site test method for building ecological irrigation canal based on microbial mineralization Download PDFInfo
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- CN110984092A CN110984092A CN201910811169.8A CN201910811169A CN110984092A CN 110984092 A CN110984092 A CN 110984092A CN 201910811169 A CN201910811169 A CN 201910811169A CN 110984092 A CN110984092 A CN 110984092A
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- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
- E02D1/00—Investigation of foundation soil in situ
- E02D1/02—Investigation of foundation soil in situ before construction work
- E02D1/022—Investigation of foundation soil in situ before construction work by investigating mechanical properties of the soil
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- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02B—HYDRAULIC ENGINEERING
- E02B13/00—Irrigation ditches, i.e. gravity flow, open channel water distribution systems
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- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
- E02D1/00—Investigation of foundation soil in situ
- E02D1/02—Investigation of foundation soil in situ before construction work
- E02D1/027—Investigation of foundation soil in situ before construction work by investigating properties relating to fluids in the soil, e.g. pore-water pressure, permeability
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Abstract
The invention discloses a field test method for simulating construction of an ecological irrigation canal based on microbial mineralization, and belongs to the technical field of hydraulic engineering. The method comprises the following steps: excavating in field to form a base surface of the irrigation ditch; dividing the irrigation ditch into a plurality of subareas by using a steel plate; processing different materials and processes on each subarea; testing the water permeability of the treated irrigation canal soil body by using a water injection method; testing the shear strength of the soil body by using a portable cross plate shearing instrument; testing the impact resistance of the soil body by using a total station; determining the material and the process of the ecological irrigation canal. The invention determines the material and the process for building the ecological irrigation canal based on the microbial mineralization by comparing the water permeability, the shear strength and the impact resistance of the soil body treated by different parameters and processes. Compared with the traditional irrigation canal construction technology, the ecological irrigation canal constructed based on the microbial mineralization avoids a series of problems of greenhouse gas release, dust pollution, construction waste, soil pollution and the like in cement production in concrete lining, and is an environment-friendly innovative technology.
Description
The technical field is as follows:
the invention relates to a field test method for building an ecological irrigation canal based on microbial mineralization, and belongs to the technical field of hydraulic engineering.
Background art:
irrigation canals are irrigation channels, which are irrigation canals used for guiding water to irrigate farmlands. At present, irrigation canals are generally constructed by lining with thin concrete, and mainly have the functions of seepage prevention, scour prevention and the like. One of the main raw materials of concrete lining is cement, which consumes more energy in the production process, releases greenhouse gases and causes dust pollution. Meanwhile, for some field irrigation canals, irrigation canal diversion is often involved in subsequent farmland transformation, and the concrete material is a hard lining, so that construction waste is formed during diversion, and even soil is possibly polluted. There is a need to develop a green and feasible ecological irrigation canal construction technology to avoid the above problems.
Microbial Mineralization (MICP) is a biological process that occurs in nature. After a large amount of urease-producing microorganisms and a cementing agent are introduced into a soil matrix, mineralization can be carried out to produce calcium carbonate. The calcium carbonate is filled in the particles, so that the permeability of the soil can be reduced, the strength and the impact resistance of the soil can be enhanced, and the aim of improving the engineering property of the soil body on the surface of the irrigation canal can be further achieved. The microorganism is a harmless bacterium naturally existing in the soil of the nature, and hardly has any influence on the soil, the human health and the environment, so the microorganism mineralization technology is an environment-friendly innovative technology.
At present, the microbial mineralization technology is still in a development stage, and experimental research is mostly limited to be carried out in a laboratory. As mentioned above, the microbial mineralization technology can effectively improve the soil engineering properties of the surface of the irrigation canal, but at present, the technology is not applied to the actual irrigation canal engineering.
The invention content is as follows:
the invention aims to solve the problems and researches materials and construction processes for building the on-site ecological irrigation canal based on microbial mineralization by designing an on-site test method for simulating building of the ecological irrigation canal based on microbial mineralization.
In order to achieve the purpose, the invention provides a field test method for building an ecological irrigation canal based on microbial mineralization, which is realized by the following technical scheme.
A field trial method for simulating the construction of an ecological irrigation canal based on microbial mineralization, the method comprising:
the method comprises the steps of firstly, excavating in a field by using an excavator to form an irrigation ditch base surface, and measuring an initial section of the irrigation ditch by using a total station.
And step two, using a steel plate to be driven into the ground to divide the irrigation canal into a plurality of watertight sub-areas.
And step three, processing different materials and construction processes for each subarea. The materials comprise bacillus pasteurii bacterial liquid with different concentrations, calcium chloride solution, urea solution and mixed solution. The construction process comprises spraying the above materials at specific time intervals, spraying for a certain number of times, and finally maintaining for a period of time.
And step four, filling each subarea with water, reading the water level change of each subarea at regular intervals, and calculating the water permeability of the processed soil body.
And step five, testing the shear strength of the soil body on the surface of the irrigation channel by using a portable cross plate shearing instrument.
And step six, pulling out the previous steel plate, supplying water at a certain flow rate to the whole irrigation channel area by using a water pump, draining accumulated water in the irrigation channel by using the water pump after flushing for a certain time, measuring the section of the irrigation channel by using a total station, and calculating the soil erosion amount according to the change of the section.
And seventhly, determining the material and the construction process for constructing the ecological irrigation canal based on microbial mineralization by comparing different parameters and the water permeability, the shear strength and the impact resistance of the soil body after the process treatment.
Compared with the prior art, the invention has the following beneficial effects:
1. the test is directly carried out on site, the problem of size effect existing in an indoor test is solved, and the obtained material and construction process parameters can be directly used for building an actual ecological irrigation canal;
2. the ecological irrigation canal constructed by the method can achieve the purposes of seepage prevention and scour prevention without conventional concrete lining, and is environment-friendly and pollution-free;
3. the ecological irrigation canal constructed by applying the method has no hard lining, and building rubbish can not be generated in the subsequent farmland reconstruction.
Description of the drawings:
FIG. 1 is a flow chart of the field test method for building an ecological irrigation canal based on microbial mineralization, provided by the invention;
FIG. 2 is a schematic diagram of a field test area for constructing an ecological irrigation canal based on microbial mineralization, provided by the invention;
FIG. 3 is a cross-sectional view of undisturbed soil formed after excavation of an irrigation canal in a test;
FIG. 4 is a cross-sectional view of the canal after microbial consolidation in the experiment.
In the figure: 1 is a steel plate, 2 is an irrigation ditch subregion, 3 is undisturbed soil, and 4 is a soil body reinforced by microorganisms.
The specific implementation mode is as follows:
in order to make the purpose, technical scheme and advantages of the method more clearly understood, the invention is further explained with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only for the purpose of illustrating the present invention and are not to be construed as limiting the present invention, since different site environments may cause some differences in construction process.
Fig. 1 shows a flow chart of a simulation field test method for building an ecological irrigation canal based on microbial mineralization, which is provided by the embodiment of the invention and detailed as follows:
in step S1, dividing a plurality of sections into sub-areas according to the actual length of the irrigation ditch base surface formed by field excavation, wherein each sub-area is about 1m long and serves as a test work area;
in step S2, the irrigation canal is divided into watertight sub-areas by driving steel plates into the ground.
FIG. 2 is a schematic view of a site trench after steel plates have been driven into the trench in accordance with an embodiment of the present invention; fig. 3 shows a cross-sectional view of undisturbed soil formed after trench digging in the test of the embodiment of the invention.
In step S3, different materials and construction processes are applied to each sub-area, wherein the materials include different concentrations of pasteurella bacteria liquid, calcium chloride solution and urea solution, and mixed solution therebetween. The construction process includes spraying one of the said materials in certain time interval and certain times. The specific details of each material are as follows:
in the embodiment of the invention, the bacterial liquid is prepared by culturing strains in a culture solution. One of the preferable examples is taken as an embodiment of the present invention, and the colony processing method is specifically described as follows:
dissolving 13.0g of a nutrient medium comprising 5.0g of peptone, 5.0g of sodium chloride (NaCl), 2.0g of yeast extract and 1.0g of meat extract in 1 liter of distilled water; then, autoclaving is carried out, and the pH value is made to be 7.0;
culturing the pasteuria bacillus in a nutrient medium at a culture temperature of 37 ℃ at a speed of 130 r/min;
subsequently, a concentration of 5X 10 was obtained7cfu/mL (5X 10 per mL)7Viable bacteria count, i.e. optical density of 1.3), i.e. 5 × 107cfu/mL of bacterial liquid. The concentration of the pasteurella is measured by a diffusion plate method.
In the examples of the present invention, calcium chloride (CaCl) was sprayed2) The solution is intended to provide the calcium ions needed to form calcium carbonate. In addition, calcium chloride (CaCl) at a specific concentration2) The solution can prevent colony from aggregating to form a cluster, and the uniformity of the colony in the solution is kept, wherein one of the preferred examples is taken as the embodiment of the invention, and the concentration of the calcium chloride solution is 0.05M, namely 0.05 mol/L.
In the present example, urea (CO (NH) was sprayed2)2) The solution is intended to provide the necessary chemical raw materials as reactants in the reaction, i.e. to participate in the bacterial hydrolysis reaction and subsequently to obtain the reaction product calcium carbonate (CaCO)3) After being gathered, the calcium carbonate can play a role in reinforcing the soil body. The chemical equation for the reaction is as follows:
besides being independently sprayed, the bacterial liquid, the calcium chloride solution and the urea solution can be mixed with each other to form a mixed solution, so that different construction materials are obtained. In the embodiment of the invention, one preferable scheme is that 0.05M of calcium chloride is mixed in the bacterial liquid to form a mixed solution A, and 0.5M of calcium chloride and 0.5M of urea are mixed to form a mixed solution B. And then, spraying soil on the surface of the irrigation canal by different construction processes.
One of the preferable construction processes is taken for detailed description: spraying the mixed solution A, spraying the mixed solution B after 6 hours, spraying the mixed solution A after 12 hours, spraying the mixed solution B after 18 hours, spraying the mixed solution B repeatedly at intervals for 12 times, and curing for 7 days. To avoid clogging the surface and to allow the solution to penetrate deep and the precipitation to occur slowly, it is ensured that per m360kg of CaCO can be generated in the soil3Therefore, the treatment is carried out by adopting a mode of spraying at intervals until the ground surface is wet.
The construction process is only one of the embodiments of the present invention, and is not limited to other construction processes of the present invention. Because the soil engineering properties of different sites are different, the reaction rates of the processed undisturbed soil are different, and the corresponding spraying construction processes are different.
In step S4, after the soil sample is reinforced, each sub-area is filled with water, and then the water level change of each sub-area is read at regular intervals to calculate the water permeability of the soil body after treatment.
Fig. 4 is a cross-sectional view of a field drench soil sample provided by an embodiment of the invention after being subjected to a microbial strengthening treatment.
In step S5, the shear strength of the treated soil was tested by randomly taking 5 points per sub-area using a portable cross-plate shear apparatus.
In step S6, the former steel plate is pulled out, a water pump is used to supply water at a certain flow rate to the whole irrigation canal area, after a certain time of flushing, the water pump is used to drain the accumulated water in the irrigation canal, then a total station is used to measure the section of the irrigation canal, and the soil erosion amount is calculated through the change of the section. The size of the soil erosion amount determines the quality of the impact resistance of the soil body, and the larger the soil erosion amount is, the worse the impact resistance is.
In step S7, an optimal microbial treatment process corresponding to the field irrigation is selected through comparative analysis of the obtained three types of test results, namely, comparative analysis of water permeability, impact resistance and shear strength of the soil sample.
Compared with the prior art, the embodiment of the invention has the following beneficial effects:
1. the test is directly carried out on site, the problem of size effect existing in an indoor test is solved, and the obtained material and construction process parameters can be directly used for building the ecological irrigation canal;
2. the ecological irrigation canal constructed by the method can achieve the purposes of seepage prevention and scour prevention without conventional concrete lining, and is environment-friendly and pollution-free;
3. the ecological irrigation canal constructed by applying the method has no hard lining, and building rubbish can not be generated in the subsequent farmland reconstruction.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (2)
1. A field test method for simulating the construction of an ecological irrigation canal based on microbial mineralization is characterized by comprising the following steps:
the method comprises the steps of firstly, excavating in a field by using an excavator to form an irrigation ditch base surface, and measuring an initial section of the irrigation ditch by using a total station.
And step two, using a steel plate to be driven into the ground to divide the irrigation canal into a plurality of watertight sub-areas.
And step three, processing different materials and construction processes for each subarea. The materials comprise bacillus pasteurii bacterial liquid with different concentrations, calcium chloride solution, urea solution and mixed solution. The construction process comprises spraying the above materials at specific time intervals, spraying for a certain number of times, and finally maintaining for a period of time.
And step four, filling each subarea with water, reading the water level change of each subarea at regular intervals, and calculating the water permeability of the processed soil body.
And step five, testing the shear strength of the soil body on the surface of the irrigation channel by using a portable cross plate shearing instrument.
And step six, pulling out the previous steel plate, supplying water at a certain flow rate to the whole irrigation channel area by using a water pump, draining accumulated water in the irrigation channel by using the water pump after flushing for a certain time, measuring the section of the irrigation channel by using a total station, and calculating the soil erosion amount according to the change of the section.
And seventhly, determining the material and the construction process for constructing the ecological irrigation canal based on microbial mineralization by comparing different parameters and the water permeability, the shear strength and the impact resistance of the soil body after the process treatment.
2. The sub-area of the irrigation canal of step one of claim 1, wherein the sub-area is a complete irrigation canal and is driven into the ground by steel plates to block water, and the irrigation canal is divided into a plurality of sub-areas which are mutually impermeable to water, and each sub-area is an independent test work area, so that comparison tests of different materials and processes can be conveniently carried out.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112431170A (en) * | 2020-11-12 | 2021-03-02 | 中交天津港湾工程研究院有限公司 | Construction method of river channel balance environment-friendly river bank structure and river bank structure |
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Cited By (1)
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