CN110982829A - Gene combination for resisting insect pests of crops and carrier and application thereof - Google Patents
Gene combination for resisting insect pests of crops and carrier and application thereof Download PDFInfo
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- CN110982829A CN110982829A CN201911337022.6A CN201911337022A CN110982829A CN 110982829 A CN110982829 A CN 110982829A CN 201911337022 A CN201911337022 A CN 201911337022A CN 110982829 A CN110982829 A CN 110982829A
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- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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Abstract
The application discloses a gene combination for resisting insect pests of crops, which is characterized in that the gene combination for resisting insect pests consists of Cry1Ab, Cry2Ab and Vip3Aa genes which are sequentially arranged in any order. The application also discloses a corresponding vector, and application of the protein and the gene in pest resistance. After the gene combination is transferred into a plant, the resistance effect of the oriental armyworm and the autumn armyworm superposed by the three genes is greatly improved, the breeding efficiency is greatly improved, and the breeding cost is reduced.
Description
Technical Field
The application relates to the technical field of genetic engineering biological control, in particular to a method for improving the insect-resistant effect of a transformed plant by artificially modifying insect-resistant genes Cry1Ab, Cry2Ab and Vip3Aa, and also relates to a corresponding expression vector and application thereof.
Background
Biological control is to control the population quantity of pests by using some beneficial organisms or biological metabolites to achieve the purpose of reducing or eliminating the pests, such as trichogramma or beauveria bassiana to control the meadow moth. It is characterized by safety to human and livestock, little pollution to environment and long-term control of certain pests; but the effect is often unstable and the same investment is required to be made no matter the weight of the meadow moth is light.
In order to solve the limitation of agricultural control, chemical control, physical control and biological control in practical application, scientists find that some insect-resistant transgenic plants can be obtained to prevent and control plant pests by transferring insect-resistant genes for coding insecticidal proteins into plants through research.
The Bt gene encodes an insecticidal crystal protein and is derived from Bacillus thuringiensis (Bacillus thuringiensis). Insecticidal parasporal crystal proteins of delta-endotoxin are produced during sporulation thereof, and these proteins have high insecticidal activity. The principle of action is that this insect-resistant protein is solubilized by alkaline intestinal fluid and hydrolyzed to smaller active toxin fragment-core fragment (Hofte and Whiteley, 1989). The active fragment is resistant to further hydrolysis by proteases, and the activated protein binds to brush vesicles on the insect gut, causing perforation to affect osmotic balance, cell swelling and lysis, and target organisms stop feeding and eventually die. Studies have shown that intestinal epithelial cells of many target pests have high affinity binding sites for Bt proteins (Hofte and Whiteley, 1989). Over the past several decades, several tens of Bacillus thuringiensis strains and 130 or more of their encoded insecticidal crystal proteins have been identified.
The Bt gene encodes an insecticidal crystal protein and is derived from Bacillus thuringiensis (Bacillus thuringiensis). Insecticidal parasporal crystal proteins of delta-endotoxin are produced during sporulation thereof, and these proteins have high insecticidal activity. The principle of action is that this insect-resistant protein is solubilized by alkaline intestinal fluid and hydrolyzed to smaller active toxin fragment-core fragment (Hofte and Whiteley, 1989). The active fragment is resistant to further hydrolysis by proteases, and the activated protein binds to brush vesicles on the insect gut, causing perforation to affect osmotic balance, cell swelling and lysis, and target organisms stop feeding and eventually die. Studies have shown that intestinal epithelial cells of many target pests have high affinity binding sites for Bt proteins (Hofte and Whiteley, 1989). Over the past several decades, several tens of Bacillus thuringiensis strains and 130 or more of their encoded insecticidal crystal proteins have been identified.
Biological control is to control the population quantity of pests by using some beneficial organisms or biological metabolites to achieve the purpose of reducing or eliminating the pests, such as trichogramma or beauveria bassiana to control the meadow moth. It is characterized by safety to human and livestock, little pollution to environment and long-term control of certain pests; but the effect is often unstable and the same investment is required to be made no matter the weight of the meadow moth is light.
In order to solve the limitation of agricultural control, chemical control, physical control and biological control in practical application, scientists find that some insect-resistant transgenic plants can be obtained to prevent and control plant pests by transferring insect-resistant genes for coding insecticidal proteins into plants through research.
Cry2Ab insecticidal protein is one of many insecticidal proteins, and Schenpf and Whiteley cloned the first Cry gene Cry1Aa1 encoding delta-endotoxin from Bacillus thuringiensis (Bacillus thuringiensis) in 1981. In 1989, Widner W.R and Whiteley H.R cloned the Cry2Ab gene from Bacillus thuringiensis (Bacillus thuringiensis). The Cry2Ab protein is ingested by the insect into the midgut and the toxoprotein protoxin is solubilized in the alkaline pH environment of the insect midgut. The N-and C-termini of the protein are digested by alkaline protease to convert the protoxin to an active fragment; the active fragment is combined with a receptor on the upper surface of the insect midgut epithelial cell membrane and is inserted into the intestinal membrane, so that the cell membrane has perforation symptoms, osmotic pressure change, pH balance and the like inside and outside the cell membrane are damaged, the digestion process of the insect is disturbed, and the insect finally dies.
Cry2Ab insecticidal protein is one of many insecticidal proteins, and Schenpf and Whiteley cloned the first Cry gene Cry1Aa1 encoding delta-endotoxin from Bacillus thuringiensis (Bacillus thuringiensis) in 1981. In 1989, Widner W.R and Whiteley H.R cloned the Cry2Ab gene from Bacillus thuringiensis (Bacillus thuringiensis). The Cry2Ab protein is ingested by the insect into the midgut and the toxoprotein protoxin is solubilized in the alkaline pH environment of the insect midgut. The N-and C-termini of the protein are digested by alkaline protease to convert the protoxin to an active fragment; the active fragment is combined with a receptor on the upper surface of the insect midgut epithelial cell membrane and is inserted into the intestinal membrane, so that the cell membrane has perforation symptoms, osmotic pressure change, pH balance and the like inside and outside the cell membrane are damaged, the digestion process of the insect is disturbed, and the insect finally dies.
The Vip3A insecticidal protein is one of a plurality of insecticidal proteins, and is a specific protein produced by Bacillus cereus. The Vip3A protein is ingested by the insect into the midgut and the toxoprotoxin is solubilized in the alkaline pH environment of the insect midgut. The N-and C-termini of the protein are digested by alkaline protease to convert the protoxin to an active fragment; the active fragment is combined with a receptor on the upper surface of the insect midgut epithelial cell membrane and is inserted into the intestinal membrane, so that the cell membrane generates perforation focus, the osmotic pressure change and the pH balance inside and outside the cell membrane are damaged, the digestion process of the insect is disturbed, and the insect finally dies. The existing transgenic plants prove that the Vip3A gene-transferred plants can resist the invasion of Lepidoptera (Lepidotera) pests such as black cutworms, spodoptera frugiperda, corn armyworm and the like. Chinese patent applications with application numbers of 201210528162.3 and 201610080690.5 respectively provide a technical scheme for transferring a Vip3A coding gene into a plant to achieve an insect-resistant effect, and the gene is also modified in 201210528162.3 to improve the insect-resistant effect.
Although there are procedures for transforming the above three genes into plants to achieve the purpose of insect resistance, there is no description in the literature of using a combination of multiple insect-resistant genes because the insect-resistant effects of the genes are different.
Disclosure of Invention
In view of the above technical problems, the present application aims to provide a gene combination formed by multiple insect-resistant gene combinations, specifically, genes mCry1Ab, mCry2Ab and mVip3Aa are combined in the same vector and transferred into a plant, and the obtained transgenic plant has a greatly improved resistance effect of oriental armyworm and autumn armyworm due to the superposition of the three genes.
The invention discloses a gene combination for resisting insect pests of crops, which consists of mCry1Ab, mCry2Ab and mVip3Aa genes which are sequentially arranged in any order. According to the invention, the resistance effect of the transgenic plant to oriental armyworm and autumn armyworm is greatly improved by overlapping three insect-resistant genes. Preferably, the three genes are connected in the order of mCry2Ab + mVip3Aa + mCry1Ab (RB to LB), which has the highest expression efficiency and the best insect-resistant effect.
In one embodiment according to the present invention, the nucleotide sequence of the mCry1Ab gene is SEQ ID NO. 1. The modified Cry1Ab gene solves the problems of easy breakage of Cry1Ab gene and poor recombination and resistance, and has more stability, higher expression efficiency and more efficient insect-resistant and insecticidal effect in application.
In one embodiment according to the present invention, the nucleotide sequence of the mCry2Ab gene is SEQ ID NO. 2. After the modified mCry2Ab gene provided by the invention is transferred into a plant, the obtained transgenic plant has better resistance to beet armyworm and prodenia litura.
In one embodiment according to the invention, the nucleotide sequence of the mVip3Aa gene is SEQ ID NO 3. The transgenic plant introduced by the modified mVip3Aa gene has less agronomic characters, the ratio of high-quality transformants is increased, and the influence on pollen is less.
The invention also provides an expression cassette, a recombinant vector, a recombinant microorganism or a transgenic cell line containing the insect-resistant gene.
The invention further provides an expression vector, which comprises the insect-resistant gene combination.
The invention further provides the gene combination, or an expression cassette, a recombinant vector, a recombinant microorganism or a transgenic cell line containing the insect-resistant gene, or an application of the expression vector in insect resistance of plants, wherein the application is selected from one or two of the following: a) preparing a medicament having an anti-insect effect; b) cultivating a transgenic plant having or having an increased ability to resist insects.
In one embodiment according to the present invention, the pest controlled in the application is selected from one or more of beet armyworm, prodenia litura and oriental armyworm.
The present invention further provides a method of growing a plant having or having improved insect resistance, said method comprising the steps of: and (3) introducing the gene combination into a receptor plant to obtain a transgenic plant.
According to one embodiment of the invention, the plant is selected from one or more of monocotyledons, dicotyledons, gramineae; preferably corn.
The invention has the following beneficial effects:
after the combination of the insect-resistant genes provided by the invention is transferred into plants, the problems of few insect-resistant mechanisms, narrow insect-resistant spectrum and easy generation of resistance by insects in the prior art are solved, and the insect-resistant genes have faster and more effective insect-resistant effect and are difficult to generate resistance. According to the invention, the three insect-resistant genes are superposed, so that the resistance effect of the transgenic plant to oriental armyworm and autumn armyworm is greatly improved, the breeding efficiency is also greatly improved, and the breeding cost is reduced.
Drawings
FIG. 1 shows a vector map for accessing mCry1Ab gene;
FIG. 2 shows a vector map for accessing the mCry2Ab gene;
FIG. 3 shows a map of a vector for accessing mVip3Aa gene;
FIG. 4 shows the vector map for accessing mCry1Ab and mCry2Ab genes;
FIG. 5 shows a vector map for accessing mCry1Ab and mVip3Aa genes;
FIG. 6 shows the vector maps for the access genes mCry1Ab, mCry2Ab and mVip3 Aa;
FIG. 7 shows a PCR detection map of the mCry1Ab gene for 12 samples;
FIG. 8 shows a PCR detection map of the mCry2Ab gene for 12 samples;
FIG. 9 is a PCR map of mVip3Aa gene from 12 samples.
Detailed Description
The following examples are intended to illustrate the present application but are not intended to limit the scope of the present application.
Specific embodiments of the present application will be described in more detail below. These embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.
In the following description and in the claims, the terms "include" and "comprise" are used in an open-ended fashion, and thus should be interpreted to mean "include, but not limited to. The description which follows is a preferred embodiment of the present application, but is made for the purpose of illustrating the general principles of the application and not for the purpose of limiting the scope of the application. The protection scope of the present application shall be subject to the definitions of the appended claims.
If not specifically stated, mCry1Ab, mCry2Ab and mVip3Aa are all brand new genes obtained by respectively modifying Cry1Ab, Cry2Ab and Vip3Aa in the invention. Wherein the nucleotide sequence of mCry1Ab is SEQ ID NO. 1, the nucleotide sequence of mCry2Ab is SEQ ID NO. 2, and the nucleotide sequence of mVip3Aa is SEQ ID NO. 3; the amino acid sequence of Cry1Ab is SEQ ID NO. 4, the amino acid sequence of Cry2Ab is SEQ ID NO. 5 and the amino acid sequence of Vip3Aa is SEQ ID NO. 6.
Example 1 vector construction: the vector is constructed by adopting Gateway system
FIGS. 1 to 6 show maps of respective vectors involved in the present invention, respectively.
BP recombination loading entry carrier
1. PCR with attB linker addition: respectively taking plasmids carrying target genes mCry1Ab, mCry2Ab and mVip3Aa expression cassettes as templates, adding gene specific primers containing partial linkers, and performing first round PCR
2. Taking the PCR product of the first round as a template, adding a universal primer, carrying out the second round of PCR, and purifying the PCR product
BP recombination reaction
Incubating for 1-16 h at 25 ℃, but not more than 18h, and stopping the reaction.
Reaction product transformed into escherichia coli
1-5 mul of BP reaction solution is taken to transform Escherichia coli T1 competent cells (Transgen, Beijing, China; Cat. No: CD501) by a heat shock method, and the heat shock conditions are as follows: 50. mu.l of E.coli T1 competent cells, 10. mu.l of plasmid DNA (recombinant cloning vector LP01-T), water bath at 42 ℃ for 30 seconds; the ampicillin (100 mg/L) coated LB plates (tryptone 10g/L, yeast extract 5g/L, NaCl10g/L, agar 15g/L, pH adjusted to 7.5 with NaOH) were grown overnight in a water bath at 37 ℃ for 45 minutes (shaking table at 200 rpm). White colonies were picked and cultured overnight in LB liquid medium (tryptone 10g/L, yeast extract 5g/L, NaCl10g/L, ampicillin 100mg/L, pH 7.5 adjusted with NaOH) at 37 ℃. Extracting the plasmid by an alkaline method: centrifuging the bacterial solution at 12000rpm for 1min, removing supernatant, and suspending the precipitated bacterial solution with 100 μ l ice-precooled solution I (25mM Tris-HCl, 10mM EDTA (ethylene diamine tetraacetic acid), 50mM glucose, pH 8.0); add 150. mu.l of freshly prepared solution II (0.2M NaOH, 1% SDS (sodium dodecyl sulfate)), invert the tube 4 times, mix, and place on ice for 3-5 min; adding 150 μ l ice-cold solution III (4M potassium acetate, 2M acetic acid), mixing well immediately, and standing on ice for 5-10 min; centrifuging at 4 deg.C and 12000rpm for 5min, adding 2 times volume of anhydrous ethanol into the supernatant, mixing, and standing at room temperature for 5 min; centrifuging at 4 deg.C and 12000rpm for 5min, removing supernatant, washing precipitate with 70% ethanol, and air drying; the precipitate was dissolved by adding 30. mu.l of RNase (20. mu.g/ml) in TE (10mM Tris-HCl, 1mM EDTA, pH 8.0); bathing in water at 37 deg.C for 30min to digest RNA; storing at-20 deg.C for use. The 1Abo, 2Ab and Vip3Aa expression cassettes were all identified as having been correctly ligated to the entry vector using appropriate enzymatic cleavage.
Recombinant LR incorporation into expression vectors
Carrying out warm bath for 1-16 h at 25 ℃, and stopping the reaction.
Reaction product transformed into escherichia coli
1-5 mul of BP reaction solution is taken to transform Escherichia coli T1 competent cells (Transgen, Beijing, China; Cat. No: CD501) by a heat shock method, and the heat shock conditions are as follows: 50. mu.l of E.coli T1 competent cells, 10. mu.l of plasmid DNA (recombinant cloning vector LP01-T), water bath at 42 ℃ for 30 seconds; the ampicillin (100 mg/L) coated LB plates (tryptone 10g/L, yeast extract 5g/L, NaCl10g/L, agar 15g/L, pH adjusted to 7.5 with NaOH) were grown overnight in a water bath at 37 ℃ for 45 minutes (shaking table at 200 rpm). White colonies were picked and cultured overnight in LB liquid medium (tryptone 10g/L, yeast extract 5g/L, NaCl10g/L, ampicillin 100mg/L, pH 7.5 adjusted with NaOH) at 37 ℃. Extracting the plasmid by an alkaline method: centrifuging the bacterial solution at 12000rpm for 1min, removing supernatant, and suspending the precipitated bacterial solution with 100 μ l ice-precooled solution I (25mM Tris-HCl, 10mM EDTA (ethylene diamine tetraacetic acid), 50mM glucose, pH 8.0); add 150. mu.l of freshly prepared solution II (0.2M NaOH, 1% SDS (sodium dodecyl sulfate)), invert the tube 4 times, mix, and place on ice for 3-5 min; adding 150 μ l ice-cold solution III (4M potassium acetate, 2M acetic acid), mixing well immediately, and standing on ice for 5-10 min; centrifuging at 4 deg.C and 12000rpm for 5min, adding 2 times volume of anhydrous ethanol into the supernatant, mixing, and standing at room temperature for 5 min; centrifuging at 4 deg.C and 12000rpm for 5min, removing supernatant, washing precipitate with 70% ethanol, and air drying; the precipitate was dissolved by adding 30. mu.l of RNase (20. mu.g/ml) in TE (10mM Tris-HCl, 1mM EDTA, pH 8.0); bathing in water at 37 deg.C for 30min to digest RNA; storing at-20 deg.C for use. Digestion with BamHI and SbfI identified that the 1Ab, 2Ab and Vip3Aa expression cassettes were all properly ligated into the final vector.
Example 2
The conversion comprises the following specific steps:
1. preparation of young maize embryos
The maize inbred line AX808 in a company is planted in a field or a greenhouse, and maize 8-10 days (summer) to 10-13 days (autumn) after artificial pollination is taken as a source of immature embryos.
2. Preparation of Agrobacterium
(1) Marking the transformed and identified agrobacterium tumefaciens glycerol on a YEP solid culture medium added with 100mg/L kan and 12mg/L tet, and culturing in dark at 28 ℃ for 2-3 days;
(2) adding 1ml of infection culture medium into a sterilized 2ml centrifugal tube, putting the agrobacterium of the step 1 into the infection culture medium, and fully scattering and uniformly mixing the agrobacterium with a pipette gun;
(3) another sterilized 2ml centrifuge tube was used to adjust the concentration of the bacterial liquid to OD 660 of 0.5-0.7 using the infection medium.
3. Co-culture of young maize embryos and agrobacterium
(1) Removing the infection culture medium in the immature embryo centrifuge tube, and adding 1.5ml of fresh infection culture medium to clean the embryo once;
(2) removing the infection culture medium, and adding the adjusted agrobacterium liquid;
(3) oscillating at the maximum rotating speed for 30s, and standing at room temperature for 5 min;
(4) pouring the embryos onto a co-culture medium, and blotting the liquid;
(5) placing the embryo with the plane upward and the shield surface upward;
(6) the embryos are cultured in the dark at 22 ℃ for 2-3 days.
4. Induction and selection of calli
(1) Transferring the co-cultured embryo to an induced callus culture medium, and performing dark culture in an incubator at 28 ℃ for 7-10 days;
(2) transferring the induced callus to a screening culture medium for screening culture, wherein the screening pressure is 5.0mM glyphosate, and dark culture is carried out for 2-3 weeks at 28 ℃;
(3) taking the callus survived in the first screening to carry out the second screening, wherein the screening pressure is 2.0 mM;
5. regeneration and culture of transformed strains
(1) Placing the screened embryogenic callus on a pre-differentiation culture medium, and performing dark culture at 28 deg.C for 10-14 days;
(2) taking embryo healing wound on a differentiation culture medium, and performing light culture at 28 ℃ for 10-14 days until the seedling is differentiated;
(3) transferring the differentiated seedling to a rooting culture medium, and performing light culture at 28 ℃ until the root is completely developed;
(4) transplanting the well-grown seedlings into a greenhouse matrix.
And harvesting the transgenic plants after the transgenic plants blossom and fruit. The harvested seeds are sowed in a greenhouse, and when the plants grow to 4-6 leaf stages, expression analysis and detection are carried out by adopting a PCR technology.
Example 3 transgenic molecule detection
(1) The maize plants with mCry1Ab, mCry2Ab and mVip3Aa genes were verified by the general PCR of 2 × EasyTaq PCR Supermix (China, Beijing, Cat: AS111-11) of the King Kogyo Co., Ltd
The PCR mCry1Ab primers were:
mCry1Ab-393F(SEQ ID NO:7)CATCCAGTTCAACGACATGA
mCry1Ab-930R(SEQ ID NO:8)GTGGGCATCGGTGTAGATAG
fragment size: 528bp by 528bp
The PCR mCry2Ab primers were:
mCry2Ab-128F(SEQ ID NO:9):AGAAGAACAACCACAGCCTG
mCry2Ab-724R(SEQ ID NO:10):TGTCGTGAAGCCTCGTATTG
fragment size 597bp
The mVip3Aa primers for PCR were:
mVip3Aa-F3(SEQ ID NO:11):TGGAGGACTACCAGACCATC
mVip3Aa-R3:(SEQ ID NO:12)GGTTCCTGATCGATGACTGAC
fragment size 550bp
The conditions for the PCR reaction were: 30 cycles, each cycle being 95 ℃ 30 ', 58 ℃ 30 ', 72 ℃ 40 '.
(2) Verification of maize plants transformed with genes mCry1Ab, mCry2Ab, mVip3Aa, mCry1Ab + mCry2Ab, mCry1Ab + mVip3Aa mCry2Ab + mVip3A, mCry1Ab + mCry2Ab + mVip3Aa by qRT-PCR
About 100mg of leaves of corn plants with nucleotide sequences of mCry1Ab, mCry2Ab, mVip3Aa, mCry1Ab + mCry2Ab, mCry1Ab + mVip3AamCry2Ab + mVip3A, and mCry1Ab + mCry2Ab + mVip3Aa are respectively taken as samples, the Genomic DNA of the leaves is extracted by easy pure Plant Genomic DNA Kit (containing RNase A) of Transgen (Transgen, Beijing, China, Cat: EE111-01), and the copy number of the 1Ab +2Ab + Vip3Aa gene is detected by a TransStart Green fluorescence quantitative PCR method. Meanwhile, wild corn plants are used as a control, and detection and analysis are carried out according to the method. The experiment was repeated 3 times and the average was taken.
(2) The specific method for detecting the copy number of the gene is as follows:
the following primers were used to detect the nucleotide sequence of mCry1 Ab:
primer 1(CF1) (SEQ ID NO: 13): GTCCTGGATGGCACTGAGTT is shown as SEQ ID NO. 13 in the sequence list;
primer 2(CR1) (SEQ ID NO: 14): GGCACATTGTTGTTCTGTGG is shown as SEQ ID NO:14 in the sequence list;
the following primers were used to detect the nucleotide sequence of mCry2 Ab:
primer 3(CF2) (SEQ ID NO: 15): TCTCCTTCATTCGTGACGTG
Primer 4(CR2) (SEQ ID NO: 16): GCCGACTGGTAGGTGTTGAT
The following primers were used to detect the mVip3Aa nucleotide sequence:
primer 5(CF3) (SEQ ID NO: 17): GATCCAGTACACCGTGAAGG
Primer 6(CR3) (SEQ ID NO: 18): TTGGTGTCCTCGTAGTGGAT
The following primers were used to detect the 18s nucleotide sequence for internal reference leveling
18srRNA-F(SEQ ID NO:19):CCATCCCTCCGTAGTTAGCTTCT
18srRNA-R(SEQ ID NO:20):CCTGTCGGCCAAGGCTATATAC
The PCR reaction system is as follows:
the PCR reaction conditions are as follows:
repeating the steps 2-3 and 40 times circularly.
Data were analyzed using SDS2.3 software (Applied Biosystems).
Experimental results show that the nucleotide sequences of mCry1, mCry2, mVip3, mCry1 + mCry2, mCry1 + mVip3, mCry1 + mCry2 + mVip3 are integrated into the chromosome group of the detected corn plant, and the corn plants into which the nucleotide sequences of mCry1, mCry2, mVip3, mCry1 + mCry2, mCry1 + mVip3, mCry2 + mVip3, mCry1 + mCry2 + mVip3 are transferred all obtain transgenic corn plants containing single-copy mCry1, mCry2, mVip3, mCry1 + mCry2, mCry1 + mVip3, mCry2 + mVip3 and mCry1 + mCry2 + mVip3 genes.
The PCR detection results are shown in FIGS. 7-9, respectively, and it can be seen that each vector was successfully transferred into the plant.
Example 4 insecticidal protein detection of transgenic maize plants
1. Content detection of insecticidal protein (mCry1Ab protein) of transgenic corn plant
The solutions involved in this experiment were as follows:
extracting a buffer solution: 8g/L NaCl, 0.2g/L KH2PO4,2.9g/L Na2HPO4·12H2O, 0.2g/L KCl, 5.5ml/L Tween 20(Tween-20), pH 7.4;
wash buffer PBST: 8g/L NaCl, 0.2g/L KH2PO4,2.9g/L Na2HPO412H2O, 0.2g/LKCl, 0.5ml/L Tween 20(Tween-20), pH 7.4;
stopping liquid: 1M HCl.
Taking fresh leaves of 3mg of single-copy corn plant with the mCry1Ab nucleotide sequence and single-copy corn plant with the Cry1Ab nucleotide sequence as samples, grinding by liquid nitrogen, adding 800 mu l of the extraction buffer solution, centrifuging for 10min at the rotating speed of 4000rpm, taking supernatant, diluting by 40 times by using the extraction buffer solution, and taking 80 mu l of diluted supernatant for ELISA detection. ELISA (enzyme-linked immunosorbent assay) kits (ENVIRLOGIX company, Cry1Ab/Cry1Ac, Cry2A and Vip3A kits) are used for detecting and analyzing the proportion of insecticidal proteins (mCry1Ab, mCry2Ab and mVip3Aa proteins) in a sample to the fresh weight of the leaves, and the specific method refers to the product specification thereof. Meanwhile, wild corn plants and corn plants which are identified as non-transgenic through fluorescent quantitative PCR are used as controls, and detection and analysis are carried out according to the method. 3 strains (1A1, 1A2 and 1A3) which are transferred into the nucleotide sequence of mCry1Ab, 3 strains (1A4, 1A5 and 1A6) which are transferred into the nucleotide sequence of mCry1Ab + mCry2Ab, 3 strains (1A7, 1A8 and 1A 9) which are transferred into the nucleotide sequence of mCry1Ab + mVip3Aa, 3 strains (1A10, 1A11 and 1A12) which are transferred into the nucleotide sequence of mCry1Ab + mCry2Ab + mVip3Aa are identified as1 strain of non-transgenic (NGM) by fluorescence quantitative PCR, and 1 strain of wild type CK (CK); 3 strains are selected from each strain for testing, each strain is repeated for 6 times, the protein content of mCry1Ab is 8453.2ng/g, 8591.0ng/g, 8487.3ng/g and 8744.9ng/g respectively, the protein content of mCry1Ab in transformants of each vector has no obvious difference, and the specific detection result is shown in Table 1.
3 strains (2A1, 2A2 and 2A3) which are transformed into mCry2Ab nucleotide sequence, 3 strains (2A4, 2A5 and 2A6) which are transformed into mCry1Ab + mCry2Ab nucleotide sequence, 3 strains (2A7, 2A8 and 2A9) which are transformed into mCry2Ab + mVip3Aa nucleotide sequence, 3 strains (2A10, 2A11 and 2A12) which are transformed into mCry1Ab + mCry2Ab + mVip3Aa nucleotide sequence are identified as non-transgenic (NGM) strains by fluorescence quantitative PCR, and 1 strain is wild type (CK); 3 strains are selected from each strain for testing, each strain is repeated for 6 times, the protein content of mCry2Ab is 3052.8ng/g, 3045.7ng/g, 2971.9ng/g and 2956.0ng/g respectively, the protein content of mCry2Ab in transformants of each vector has no obvious difference, and the specific detection result is shown in Table 2.
3 strains (3V1, 3V2 and 3V3) with mVip3Aa nucleotide sequence, 3 strains (3V4, 3V5 and 3V6) with mCry1Ab + mVip3Aa nucleotide sequence, 3 strains (3V7, 3V8 and 3V9) with mCry2Ab + mVip3Aa nucleotide sequence, 3 strains (3V10, 3V11 and 3V12) with mCry1Ab + mCry2Ab + mVip3Aa nucleotide sequence are identified as1 strain of non-transgenic (NGM) and 1 strain of wild type (CK) by fluorescence quantitative PCR; 3 strains are selected from each strain for testing, each strain is repeated for 6 times, the mVip3Aa protein content is 3118.9ng/g, 2870.8ng/g, 3011.7ng/g and 3053.9ng/g respectively, the mVip3Aa protein content in transformants of each vector has no obvious difference, and the specific detection result is shown in Table 3.
TABLE 1 average results of mCry1Ab protein expression measurements of transgenic maize plants
TABLE 2 average results of mCry2Ab protein expression measurements of transgenic maize plants
TABLE 3 mean results of mVip3Aa protein expression measurements of transgenic maize plants
Example 5 detection of insect-resistant Effect of transgenic maize plants
And carrying out insect-resistant effect detection on athetis lepigone by using the corn plant, wild corn plant and corn plant identified as non-transgenic by PCR, which are transferred with the nucleotide sequence of mCry1Ab + mCry2Ab + mVip3 Aa.
Fresh leaves of corn plants, wild-type corn plants and corn plants (V3-V4) which are identified as non-transgenic by PCR and are transferred into nucleotide sequences of mCry1Ab, mCry2Ab, mVip3Aa, mCry1Ab + mCry2Ab, mCry1Ab + mVip3AamCry2Ab + mVip3A, mCry1Ab + mCry2Ab + mVip3Aa are respectively taken, the leaves are washed clean by sterile water and the water on the leaves is sucked by filter paper, then the leaves of the corn are cut off, meanwhile, the leaves are cut into 1cm multiplied by 4cm, 2 cut long-strip-shaped leaves are put on the filter paper at the bottom of a circular plastic culture dish, the filter paper is wetted by distilled water, 5 artificially-fed oriental armyworm and two-age meadow moth are put in each culture dish, and the culture dish is covered by a test moth test plate at the temperature of 22-26 ℃, the relative humidity of 70% -80%, and the light period (light/dark period of 16): after standing for 3 days under the condition of 8, the mortality was counted. The results are shown in tables 4 and 5.
TABLE 4 biological test results of two instars of Oriental mythimna
Note: wherein 1A refers to mCry1Ab gene, 2A refers to mCry2Ab gene, and V3 refers to mVip3Aa gene.
TABLE 5 bioassay results for two-year Spodoptera frugiperda
Note: wherein 1A refers to mCry1Ab gene, 2A refers to mCry2Ab gene, and V3 refers to mVip3Aa gene.
Although the present application has been described in detail with respect to the general description and the specific examples, it will be apparent to those skilled in the art that certain changes and modifications may be made based on the present application. Accordingly, such modifications and improvements are intended to be within the scope of this invention as claimed.
Sequence listing
<110> Longping Biotechnology (Hainan) Co., Ltd
<120> gene combination for crop pest resistance, and vector and application thereof
<130>201904
<141>2019-12-19
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cccactaacc cagctctccg cgaggagatg cgcatccagt tcaacgacat gaacagcgcc 420
ctgaccaccg ccatcccact cttcgccgtc cagaactacc aagtcccgct cctgtccgtg 480
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ctggacattg tgtccctctt cccgaactac gactcccgca cctacccgat ccgcaccgtg 780
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aggggcagcg cccagggcat cgagggctcc atcaggagcc cacacctgat ggacatcctc 900
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agcaactcgt ccgtgagcat catcagagct cctatgttct cctggattca tcgcagcgcg 1380
gagttcaaca atatcattcc gtcctcccaa atcacccaaa tccccctcac caagtccacc 1440
aacctgggca gcggcacctc cgtggtgaag ggcccaggct tcacgggcgg cgacatcctg 1500
cgcaggacct ccccgggcca gatcagcacc ctccgcgtca acatcaccgc tcccctgtcc 1560
cagaggtacc gcgtcaggat tcgctacgct agcaccacca acctgcaatt ccacacctcc 1620
atcgacggca ggccgatcaa tcagggtaac ttctccgcca ccatgtccag cggcagcaac 1680
ctccaatccg gcagcttccg caccgtgggt ttcaccaccc ccttcaactt ctccaacggc 1740
tccagcgttt tcaccctgag cgcccacgtg ttcaattccg gcaatgaggt gtacattgac 1800
cgcattgagt tcgtgccagc cgaggtcacc ttcgaagccg agtacgacct ggagagagcc 1860
cagaaggctg tcaatgagct cttcacgtcc agcaatcaga tcggcctgaa gaccgacgtc 1920
actgactacc acatcgacca agtctccaac ctcgtggagt gcctctccga tgagttctgc 1980
ctcgacgaga agaaggagct gtccgagaag gtgaagcatg ccaagcgtct cagcgacgag 2040
aggaatctcc tccaggaccc caatttccgc ggcatcaaca ggcagctcga ccgcggctgg 2100
cgcggcagca ccgacatcac gatccagggc ggcgacgatg tgttcaagga gaactacgtg 2160
actctcctgg gcactttcga cgagtgctac cctacctact tgtaccagaa gatcgatgag 2220
tccaagctca aggcttacac tcgctaccag ctccgcggct acatcgaaga cagccaagac 2280
ctcgagattt acctgatccg ctacaacgcc aagcacgaga ccgtcaacgt gcccggtact 2340
ggttccctct ggccgctgag cgcccccagc ccgatcggca agtgtgccca ccacagccac 2400
cacttctcct tggacatcga tgtgggctgc accgacctga acgaggacct gggctag 2457
<210>2
<211>1905
<212>DNA
<213>Artificial Sequence
<400>2
atggacaact ccgtcctgaa ctctggtcgc accaccatct gcgacgccta caacgtcgcg 60
gcgcatgatc cattcagctt ccagcacaag agcctcgaca ctgttcagaa ggagtggacg 120
gagtggaaga agaacaacca cagcctgtac gtggaccccg tcgtcggcac ggtggccagc 180
ttccttctca agaaggtcgg ctctctcgtc gggaagcgca tcctctcgga actccgcaac 240
ctgatctttc catctggctc caccaacctc atgcaagaca tcctcaggga gaccgagaag 300
tttctcaacc agcgcctcaa cactgatacc cttgctcgcg tcaacgctga gctgacgggt 360
ctgcaagcaa acgtggagga gttcaaccgc caagtggaca acttcctcaa ccccaaccgc 420
aatgcggtgc ctctgtccat cacttcttcc gtgaacacca tgcaacaact gttcctcaac 480
cgcttgcctc agttccagat gcaaggctac cagctgctcc tgctgccact ctttgctcag 540
gctgccaacc tgcacctctc cttcattcgt gacgtgatcc tcaacgctga cgagtggggc 600
atctctgcag ccacgctgag gacctaccgc gactacctga agaactacac cagggactac 660
tccaactatt gcatcaacac ctaccagtcg gccttcaagg gcctcaatac gaggcttcac 720
gacatgctgg agttcaggac ctacatgttc ctgaacgtgt tcgagtacgt cagcatctgg 780
tcgctcttca agtaccagag cctgctggtg tccagcggcg ccaacctcta cgccagcggc 840
tctggtcccc aacaaactca gagcttcacc agccaggact ggccattcct gtattcgttg 900
ttccaagtca actccaacta cgtcctcaac ggcttctctg gtgctcgcct ctccaacacc 960
ttccccaaca ttgttggcct ccccggctcc accacaactc atgctctgct tgctgccaga 1020
gtgaactact ccggcggcat ctcgagcggc gacattggtg catcgccgtt caaccagaac 1080
ttcaactgct ccaccttcct gccgccgctg ctcaccccgt tcgtgaggtc ctggctcgac 1140
agcggctccg accgcgaggg cgtggccacc gtcaccaact ggcaaaccga gtccttcgag 1200
accacccttg gcctccggag cggcgccttc acggcgcgtg gaaattctaa ctacttcccc 1260
gactacttca tcaggaacat ctctggtgtt cctctcgtcg tccgcaacga ggacctccgc 1320
cgtccactgc actacaacga gatcaggaac atcgcctctc cgtccgggac gcccggaggt 1380
gcaagggcgt acatggtgag cgtccataac aggaagaaca acatccacgc tgtgcatgag 1440
aacggctcca tgatccacct ggcgcccaat gattacaccg gcttcaccat ctctccaatc 1500
cacgccaccc aagtgaacaa ccagacacgc accttcatct ccgagaagtt cggcaaccag 1560
ggcgactccc tgaggttcga gcagaacaac accaccgcca ggtacaccct gcgcggcaac 1620
ggcaacagct acaacctgta cctgcgcgtc agctccattg gcaactccac catcagggtc 1680
accatcaacg ggagggtgta cacagccacc aatgtgaaca cgacgaccaa caatgatggc 1740
gtcaacgaca acggcgcccg cttcagcgac atcaacattg gcaacgtggt ggccagcagc 1800
aactccgacg tcccgctgga catcaacgtg accctgaact ctggcaccca gttcgacctc 1860
atgaacatca tgctggtgcc aactaacatc tcgccgctgt actga 1905
<210>3
<211>2370
<212>DNA
<213>Artificial Sequence
<400>3
atgaacatga acaacaccaa gctgaacgcc cgcgccctgc cgagcttcat cgactacttc 60
aacggcatct acggcttcgc caccggcatc aaggacatca tgaacatgat cttcaagacc 120
gacaccggcg gcgacctgac cctggacgag atcctgaaga accagcagct gctgaacgac 180
atcagcggca agctggacgg cgtgaacggc agcctgaacg acctgatcgc ccagggcaac 240
ctgaacaccg agctgagcaa ggagatcctt aagatcgcca acgagcagaa ccaggtgctg 300
aacgacgtga acaacaagct ggacgccatc aacaccatgc tgcgcgtgta cctgccgaag 360
atcaccagca tgctgagcga cgtgctcaag cagaactacg ccctgagcct gcagatcgag 420
tacctgagca agcagctgca ggagatcagc gacaagctgg acatcatcaa cgtgaacgtc 480
ctgatcaaca gcaccctgac cgagatcaccccggcctacc agcgcatcaa gtacgtgaac 540
gagaagttcg aagagctgac cttcgccacc gagaccagca gcaaggtgaa gaaggacggc 600
agcccggccg acatcctgga cgagctgacc gagctgaccg agctggcgaa gagcgtgacc 660
aagaacgacg tggacggctt cgagttctac ctgaacacct tccacgacgt gatggtgggc 720
aacaacctgt tcggccgcag cgccctgaag accgccagcg agctgatcac caaggagaac 780
gtgaagacca gcggcagcga ggtgggcaac gtgtacaact tcctgatcgt gctgaccgcc 840
ctgcaggcca aggccttcct gaccctgacc acctgtcgca agctgctggg cctggccgac 900
atcgactaca ccagcatcat gaacgagcac ttgaacaagg agaaggagga gttccgcgtg 960
aacatcctgc cgaccctgag caacaccttc agcaacccga actacgccaa ggtgaagggc 1020
agcgacgagg acgccaagat gatcgtggag gctaagccgg gccacgcgtt gatcggcttc 1080
gagatcagca acgacagcat caccgtgctg aaggtgtacg aggccaagct gaagcagaac 1140
taccaggtgg acaaggacag cttgagcgag gtgatctacg gcgacatgga caagctgctg 1200
tgtccggacc agagcgagca aatctactac accaacaaca tcgtgttccc gaacgagtac 1260
gtgatcacca agatcgactt caccaagaag atgaagaccc tgcgctacga ggtgaccgcc 1320
aacttctacg acagcagcac cggcgagatc gacctgaaca agaagaaggt ggagagcagc 1380
gaggccgagt accgcaccct gagcgcgaac gacgacggcg tctacatgcc actgggcgtg 1440
atcagcgaga ccttcctgac cccgatcaac ggctttggcc tgcaggccga cgagaacagc 1500
cgcctgatca ccctgacctg taagagctac ctgcgcgagc tgctgctagc caccgacctg 1560
agcaacaagg agaccaagct gatcgtgcca ccgagcggct tcatcagcaacatcgtggag 1620
aacggcagca tcgaggagga caacctggag ccgtggaagg ccaacaacaa gaacgcctac 1680
gtcgaccaca ccggcggcgt gaacggcacc aaggccctgt acgtgcacaa ggacggcggc 1740
atcagccagt tcatcggcga caagctgaag ccgaagaccg agtacgtgat ccagtacacc 1800
gtgaagggca agccatcgat tcacctgaag gacgagaaca ccggctacat ccactacgag 1860
gacaccaaca acaacctgga ggactaccag accatcaaca agcgcttcac caccggcacc 1920
gacctgaagg gcgtgtacct gatcctgaag agccagaacg gcgacgaggc ctggggcgac 1980
aacttcatca tcctggagat cagcccgagc gagaagctgc tgagcccgga gctgatcaac 2040
accaacaact ggaccagcac cggcagcacc aacatcagcg gcaacaccct gaccctgtac 2100
cagggcggcc gcggcatcct gaagcagaac ctgcagctgg acagcttcag cacctaccgc 2160
gtgtacttca gcgtgagcgg cgacgccaac gtgcgcatcc gcaactcccg cgaggtgctg 2220
ttcgagaaga ggtacatgag cggcgccaag gacgtgagcg agatgttcac caccaagttc 2280
gagaaggaca acttctacat cgagctgagc cagggcaaca acctgtacgg cggcccgatc 2340
gtgcacttct acgacgtgag catcaagtag 2370
<210>4
<211>818
<212>PRT
<213>Artificial Sequence
<400>4
Met Asp Asn Asn Pro Asn Ile Asn Glu Cys Ile Pro Tyr Asn Cys Leu
1 5 10 15
Ser Asn Pro Glu Val Glu Val Leu Gly Gly Glu Arg Ile Glu Thr Gly
20 25 30
Tyr Thr Pro Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser
35 40 45
Glu Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu Val Asp Ile Ile
50 55 60
Trp Gly Ile Phe Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile
65 70 75 80
Glu Gln Leu Ile Asn Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala
85 90 95
Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Gln Ile Tyr Ala Glu
100 105 110
Ser Phe Arg Glu Trp Glu Ala Asp Pro Thr Asn Pro Ala Leu Arg Glu
115 120 125
Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Thr Thr Ala
130 135 140
Ile Pro Leu Phe Ala Val Gln Asn Tyr Gln Val Pro Leu Leu Ser Val
145 150 155 160
Tyr Val Gln Ala Ala Asn Leu His Leu Ser Val Leu Arg Asp Val Ser
165 170 175
Val Phe Gly Gln Arg Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser Arg
180 185 190
Tyr Asn Asp Leu Thr Arg Leu Ile Gly Asn Tyr Thr Asp His Ala Val
195 200 205
Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp Ser Arg
210 215 220
Asp Trp Ile Arg Tyr Asn Gln Phe Arg Arg Glu Leu Thr Leu Thr Val
225 230 235 240
Leu Asp Ile Val Ser Leu Phe Pro Asn Tyr Asp Ser Arg Thr Tyr Pro
245 250 255
Ile Arg Thr Val Ser Gln Leu Thr Arg Glu Ile Tyr Thr Asn Pro Val
260 265 270
Leu Glu Asn Phe Asp Gly Ser Phe Arg Gly Ser Ala Gln Gly Ile Glu
275 280 285
Gly Ser Ile Arg Ser Pro His Leu Met Asp Ile Leu Asn Ser Ile Thr
290 295 300
Ile Tyr Thr Asp Ala His Arg Gly Glu Tyr Tyr Trp Ser Gly His Gln
305 310 315 320
Ile Met Ala Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe Pro
325 330 335
Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro Gln Gln Arg Ile Val Ala
340 345 350
Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser Thr Leu Tyr Arg
355 360 365
Arg Pro Phe Asn Ile Gly Ile Asn Asn Gln Gln Leu Ser Val Leu Asp
370 375 380
Gly Thr Glu Phe Ala Tyr Gly Thr Ser Ser Asn Leu Pro Ser Ala Val
385 390 395 400
Tyr Arg Lys Ser Gly Thr Val Asp Ser Leu Asp Glu Ile Pro Pro Gln
405 410 415
Asn Asn Asn Val Pro Pro Arg Gln Gly Phe Ser His Arg Leu Ser His
420 425 430
Val Ser Met Phe Arg Ser Gly Phe Ser Asn Ser Ser Val Ser Ile Ile
435 440 445
Arg Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe Asn Asn
450 455 460
Ile Ile Pro Ser Ser Gln Ile Thr Gln Ile Pro Leu Thr Lys Ser Thr
465 470 475 480
Asn Leu Gly Ser Gly Thr Ser Val Val Lys Gly Pro Gly Phe Thr Gly
485 490 495
Gly Asp Ile Leu Arg Arg Thr Ser Pro Gly Gln Ile Ser Thr Leu Arg
500 505 510
Val Asn Ile Thr Ala Pro Leu Ser Gln Arg Tyr Arg Val Arg Ile Arg
515 520 525
Tyr Ala Ser Thr Thr Asn Leu Gln Phe His Thr Ser Ile Asp Gly Arg
530 535 540
Pro Ile Asn Gln Gly Asn Phe Ser Ala Thr Met Ser Ser Gly Ser Asn
545 550 555 560
Leu Gln Ser Gly Ser Phe Arg Thr Val Gly Phe Thr Thr Pro Phe Asn
565 570 575
Phe Ser Asn Gly Ser Ser Val Phe Thr Leu Ser Ala His Val Phe Asn
580 585 590
Ser Gly Asn Glu Val Tyr Ile Asp Arg Ile Glu Phe Val Pro Ala Glu
595 600 605
Val Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala Val
610 615 620
Asn Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val
625 630 635 640
Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser
645 650 655
Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys
660 665 670
His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn
675 680 685
Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr
690 695 700
Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val
705 710 715 720
Thr Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln
725 730 735
Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg
740 745 750
Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr
755 760 765
Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp
770 775 780
Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Ala His His Ser His
785 790 795 800
His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp
805 810 815
Leu Gly
<210>5
<211>634
<212>PRT
<213>Artificial Sequence
<400>5
Met Asp Asn Ser Val Leu Asn Ser Gly Arg Thr Thr Ile Cys Asp Ala
1 5 10 15
Tyr Asn Val Ala Ala His Asp Pro Phe Ser Phe Gln His Lys Ser Leu
20 25 30
Asp Thr Val Gln Lys Glu Trp Thr Glu Trp Lys Lys Asn Asn His Ser
35 40 45
Leu Tyr Val Asp Pro Val Val Gly Thr Val Ala Ser Phe Leu Leu Lys
50 55 60
Lys Val Gly Ser Leu Val Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn
65 70 75 80
Leu Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Gln Asp Ile Leu Arg
85 90 95
Glu Thr Glu Lys Phe Leu Asn Gln Arg Leu Asn Thr Asp Thr Leu Ala
100 105 110
Arg Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe
115 120 125
Asn Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro
130 135 140
Leu Ser Ile Thr Ser Ser Val Asn Thr Met Gln Gln Leu Phe Leu Asn
145 150 155 160
Arg Leu Pro Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro
165 170 175
Leu Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val
180 185 190
Ile Leu Asn Ala Asp Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr
195 200 205
Tyr Arg Asp Tyr Leu Lys Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys
210 215 220
Ile Asn Thr Tyr Gln Ser Ala Phe Lys Gly Leu Asn Thr Arg Leu His
225 230 235 240
Asp Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr
245 250 255
Val Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser
260 265 270
Gly Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Ser
275 280 285
Phe Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn
290 295 300
Ser Asn Tyr Val Leu Asn Gly Phe Ser Gly Ala Arg Leu Ser Asn Thr
305 310 315 320
Phe Pro Asn Ile Val Gly Leu Pro Gly Ser Thr Thr Thr His Ala Leu
325 330 335
Leu Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Ser Ser Gly Asp Ile
340 345 350
Gly Ala Ser Pro Phe Asn Gln Asn Phe Asn Cys Ser Thr Phe Leu Pro
355 360 365
Pro Leu Leu Thr Pro Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp
370 375 380
Arg Glu Gly Val Ala Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu
385 390 395 400
Thr Thr Leu Gly Leu Arg Ser Gly Ala Phe Thr Ala Arg Gly Asn Ser
405 410 415
Asn Tyr Phe Pro Asp Tyr Phe Ile Arg Asn Ile Ser Gly Val Pro Leu
420 425 430
Val Val Arg Asn Glu Asp Leu Arg Arg Pro Leu His Tyr Asn Glu Ile
435 440 445
Arg Asn Ile Ala Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr
450 455 460
Met Val Ser Val His Asn Arg Lys Asn Asn Ile His Ala Val His Glu
465 470 475 480
Asn Gly Ser Met Ile His Leu Ala Pro Asn Asp Tyr Thr Gly Phe Thr
485 490 495
Ile Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe
500 505 510
Ile Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln
515 520 525
Asn Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr
530 535 540
Asn Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val
545 550 555 560
Thr Ile Asn Gly Arg Val Tyr Thr Ala Thr Asn Val Asn Thr Thr Thr
565 570 575
Asn Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn
580 585 590
Ile Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile
595 600 605
Asn Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met
610 615 620
Leu Val Pro Thr Asn Ile Ser Pro Leu Tyr
625 630
<210>6
<211>789
<212>PRT
<213>Artificial Sequence
<400>6
Met Asn Met Asn Asn Thr Lys Leu Asn Ala Arg Ala Leu Pro Ser Phe
1 5 10 15
Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys Asp
20 25 30
Ile Met Asn Met Ile Phe Lys Thr Asp Thr Gly Gly Asp Leu Thr Leu
35 40 45
Asp Glu Ile Leu Lys Asn Gln Gln Leu Leu Asn Asp Ile Ser Gly Lys
50 55 60
Leu Asp Gly Val Asn Gly Ser Leu Asn Asp Leu Ile Ala Gln Gly Asn
65 70 75 80
Leu Asn Thr Glu Leu Ser Lys Glu Ile Leu Lys Ile Ala Asn Glu Gln
85 90 95
Asn Gln Val Leu Asn Asp Val Asn Asn Lys Leu Asp Ala Ile Asn Thr
100 105 110
Met Leu Arg Val Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser Asp Val
115 120 125
Leu Lys Gln Asn Tyr Ala Leu Ser Leu Gln Ile Glu Tyr Leu Ser Lys
130 135 140
Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp Ile Ile Asn Val Asn Val
145 150 155 160
Leu Ile Asn Ser Thr Leu Thr Glu Ile Thr Pro Ala Tyr Gln Arg Ile
165 170 175
Lys Tyr Val Asn Glu Lys Phe Glu Glu Leu Thr Phe Ala Thr Glu Thr
180 185 190
Ser Ser Lys Val Lys Lys Asp Gly Ser Pro Ala Asp Ile Leu Asp Glu
195 200 205
Leu Thr Glu Leu Thr Glu Leu Ala Lys Ser Val Thr Lys Asn Asp Val
210 215 220
Asp Gly Phe Glu Phe Tyr Leu Asn Thr Phe His Asp Val Met Val Gly
225 230 235 240
Asn Asn Leu Phe Gly Arg Ser Ala Leu Lys Thr Ala Ser Glu Leu Ile
245 250 255
Thr Lys Glu Asn Val Lys Thr Ser Gly Ser Glu Val Gly Asn Val Tyr
260 265 270
Asn Phe Leu Ile Val Leu Thr Ala Leu Gln Ala Lys Ala Phe Leu Thr
275 280 285
Leu Thr Thr Cys Arg Lys Leu Leu Gly Leu Ala Asp Ile Asp Tyr Thr
290 295 300
Ser Ile Met Asn Glu His Leu Asn Lys Glu Lys Glu Glu Phe Arg Val
305 310 315 320
Asn Ile Leu Pro Thr Leu Ser Asn Thr Phe Ser Asn Pro Asn Tyr Ala
325 330 335
Lys Val Lys Gly Ser Asp Glu Asp Ala Lys Met Ile Val Glu Ala Lys
340 345 350
Pro Gly His Ala Leu Ile Gly Phe Glu Ile Ser Asn Asp Ser Ile Thr
355 360 365
Val Leu Lys Val Tyr Glu Ala Lys Leu Lys Gln Asn Tyr Gln Val Asp
370 375 380
Lys Asp Ser Leu Ser Glu Val Ile Tyr Gly Asp Met Asp Lys Leu Leu
385 390 395 400
Cys Pro Asp Gln Ser Glu Gln Ile Tyr Tyr Thr Asn Asn Ile Val Phe
405 410 415
Pro Asn Glu Tyr Val Ile Thr Lys Ile Asp Phe Thr Lys Lys Met Lys
420 425 430
Thr Leu Arg Tyr Glu Val Thr Ala Asn Phe Tyr Asp Ser Ser Thr Gly
435 440 445
Glu Ile Asp Leu Asn Lys Lys Lys Val Glu Ser Ser Glu Ala Glu Tyr
450 455 460
Arg Thr Leu Ser Ala Asn Asp Asp Gly Val Tyr Met Pro Leu Gly Val
465 470 475 480
Ile Ser Glu Thr Phe Leu Thr Pro Ile Asn Gly Phe Gly Leu Gln Ala
485 490 495
Asp Glu Asn Ser Arg Leu Ile Thr Leu Thr Cys Lys Ser Tyr Leu Arg
500 505 510
Glu Leu Leu Leu Ala Thr Asp Leu Ser Asn Lys Glu Thr Lys Leu Ile
515 520 525
Val Pro Pro Ser Gly Phe Ile Ser Asn Ile Val Glu Asn Gly Ser Ile
530 535 540
Glu Glu Asp Asn Leu Glu Pro Trp Lys Ala Asn Asn Lys Asn Ala Tyr
545 550 555 560
Val Asp His Thr Gly Gly Val Asn Gly Thr Lys Ala Leu Tyr Val His
565 570 575
Lys Asp Gly Gly Ile Ser Gln Phe Ile Gly Asp Lys Leu Lys Pro Lys
580 585 590
Thr Glu Tyr Val Ile Gln Tyr Thr Val Lys Gly Lys Pro Ser Ile His
595 600 605
Leu Lys Asp Glu Asn Thr Gly Tyr Ile His Tyr Glu Asp Thr Asn Asn
610 615 620
Asn Leu Glu Asp Tyr Gln Thr Ile Asn Lys Arg Phe Thr Thr Gly Thr
625 630 635 640
Asp Leu Lys Gly Val Tyr Leu Ile Leu Lys Ser Gln Asn Gly Asp Glu
645 650 655
Ala Trp Gly Asp Asn Phe Ile Ile Leu Glu Ile Ser Pro Ser Glu Lys
660 665 670
Leu Leu Ser Pro Glu Leu Ile Asn Thr Asn Asn Trp Thr Ser Thr Gly
675 680 685
Ser Thr Asn Ile Ser Gly Asn Thr Leu Thr Leu Tyr Gln Gly Gly Arg
690 695 700
Gly Ile Leu Lys Gln Asn Leu Gln Leu Asp Ser Phe Ser Thr Tyr Arg
705 710 715 720
Val Tyr Phe Ser Val Ser Gly Asp Ala Asn Val Arg Ile Arg Asn Ser
725 730 735
Arg Glu Val Leu Phe Glu Lys Arg Tyr Met Ser Gly Ala Lys Asp Val
740 745 750
Ser Glu Met Phe Thr Thr Lys Phe Glu Lys Asp Asn Phe Tyr Ile Glu
755 760 765
Leu Ser Gln Gly Asn Asn Leu Tyr Gly Gly Pro Ile Val His Phe Tyr
770 775 780
Asp Val Ser Ile Lys
785
<210>7
<211>20
<212>DNA
<213>Artificial Sequence
<400>7
catccagttc aacgacatga 20
<210>8
<211>20
<212>DNA
<213>Artificial Sequence
<400>8
gtgggcatcg gtgtagatag 20
<210>9
<211>20
<212>DNA
<213>Artificial Sequence
<400>9
agaagaacaa ccacagcctg 20
<210>10
<211>20
<212>DNA
<213>Artificial Sequence
<400>10
tgtcgtgaag cctcgtattg 20
<210>11
<211>20
<212>DNA
<213>Artificial Sequence
<400>11
tggaggacta ccagaccatc 20
<210>12
<211>21
<212>DNA
<213>Artificial Sequence
<400>12
ggttcctgat cgatgactga c 21
<210>13
<211>20
<212>DNA
<213>Artificial Sequence
<400>13
gtcctggatg gcactgagtt 20
<210>14
<211>20
<212>DNA
<213>Artificial Sequence
<400>14
ggcacattgt tgttctgtgg 20
<210>15
<211>20
<212>DNA
<213>Artificial Sequence
<400>15
tctccttcat tcgtgacgtg 20
<210>16
<211>20
<212>DNA
<213>Artificial Sequence
<400>16
gccgactggt aggtgttgat 20
<210>17
<211>20
<212>DNA
<213>Artificial Sequence
<400>17
gatccagtac accgtgaagg 20
<210>18
<211>20
<212>DNA
<213>Artificial Sequence
<400>18
ttggtgtcct cgtagtggat 20
<210>19
<211>23
<212>DNA
<213>Artificial Sequence
<400>19
ccatccctcc gtagttagct tct 23
<210>20
<211>22
<212>DNA
<213>Artificial Sequence
<400>20
cctgtcggcc aaggctatat ac 22
Claims (10)
1. A gene combination for resisting insect pests of crops, which is characterized by consisting of mCry1Ab, mCry2Ab and mVip3Aa genes which are arranged in any sequence.
2. The gene combination of claim 1, wherein the nucleotide sequence of mCry1Ab gene is seq id No. 1.
3. The gene combination of claim 1 or 2, wherein the nucleotide sequence of mCry2Ab gene is SEQ ID No. 2.
4. A gene combination according to any one of claims 1 to 3 wherein the nucleotide sequence of the mVip3Aa gene is SEQ ID NO 3.
5. An expression cassette, recombinant vector, recombinant microorganism or transgenic cell line comprising the insect-resistant gene according to any one of claims 1 to 4.
6. An expression vector comprising the insect-resistant gene combination according to any one of claims 1 to 4.
7. The gene combination according to any one of claims 1 to 4, or an expression cassette, a recombinant vector, a recombinant microorganism or a transgenic cell line containing the insect-resistant gene according to claim 5, or an expression vector according to claim 6, wherein the application is selected from two or one of the following: a) preparing a medicament having an anti-insect effect; b) cultivating a transgenic plant having or having an increased ability to resist insects.
8. The use as claimed in claim 7, wherein the pests to be controlled in the use are selected from one or more of beet armyworm, prodenia litura and oriental armyworm.
9. A method of growing a plant having or having enhanced resistance to a pest, said method comprising the steps of: introducing the gene combination according to any one of claims 1 to 4 into a recipient plant to obtain a transgenic plant.
10. The method of claim 8 or 9, wherein the plant is selected from one or more of a monocot, a dicot, a graminaceous plant; preferably corn.
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2022094790A1 (en) * | 2020-11-04 | 2022-05-12 | 中国农业大学 | Corn event 2a-7 and identification method therefor |
Citations (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102533791A (en) * | 2009-12-03 | 2012-07-04 | 中国农业科学院植物保护研究所 | Reconstructed mvip3Aa11 gene and application thereof |
US20120324606A1 (en) * | 2009-12-16 | 2012-12-20 | Dow Agrosciences Llc | Use of cry 1ab in combination with cry1be for management of resistant insects |
US20140366786A1 (en) * | 2013-06-14 | 2014-12-18 | Monsanto Technology Llc | Differential maturing refuge and methods thereof |
CN104313036A (en) * | 2014-09-19 | 2015-01-28 | 中国农业大学 | Insect resistant gene mCry2Ab and application thereof |
CN104522056A (en) * | 2014-12-22 | 2015-04-22 | 北京大北农科技集团股份有限公司 | Application of insecticidal protein |
CN104744576A (en) * | 2015-04-16 | 2015-07-01 | 中国农业科学院植物保护研究所 | Bt (Bacillus thuringiensis) protein with insecticidal activity to lymantria dispar and application of Bt protein |
US20150246954A1 (en) * | 2002-03-22 | 2015-09-03 | Bayer Cropscience N.V. | Insecticidal proteins derived from bacillus thuringiensis |
US20170029844A1 (en) * | 2015-07-23 | 2017-02-02 | Monsanto Technology Llc | Multi functional toxins |
CN107383177A (en) * | 2017-08-16 | 2017-11-24 | 中国农业大学 | The artificial synthesized Bt killing genes mcry1Ab for transgenic anti-insect plants |
US20180208631A1 (en) * | 2017-01-12 | 2018-07-26 | Monsanto Technology Llc | Pesticidal toxin proteins active against lepidopteran insects |
US20180291395A1 (en) * | 2017-04-03 | 2018-10-11 | Monsanto Technology Llc | Novel insect inhibitory proteins |
CN109152347A (en) * | 2016-04-19 | 2019-01-04 | 美国陶氏益农公司 | For managing the combination of four kinds of VIP and CRY proteotoxins of the insect pest in plant |
CN109971881A (en) * | 2019-04-11 | 2019-07-05 | 武汉大学 | A kind of primer pair and method for identifying multivalence transgenic pest-resistant rice genotype |
-
2019
- 2019-12-23 CN CN201911337022.6A patent/CN110982829B/en active Active
Patent Citations (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20150246954A1 (en) * | 2002-03-22 | 2015-09-03 | Bayer Cropscience N.V. | Insecticidal proteins derived from bacillus thuringiensis |
CN102533791A (en) * | 2009-12-03 | 2012-07-04 | 中国农业科学院植物保护研究所 | Reconstructed mvip3Aa11 gene and application thereof |
US20120324606A1 (en) * | 2009-12-16 | 2012-12-20 | Dow Agrosciences Llc | Use of cry 1ab in combination with cry1be for management of resistant insects |
US20140366786A1 (en) * | 2013-06-14 | 2014-12-18 | Monsanto Technology Llc | Differential maturing refuge and methods thereof |
CN104313036A (en) * | 2014-09-19 | 2015-01-28 | 中国农业大学 | Insect resistant gene mCry2Ab and application thereof |
CN104522056A (en) * | 2014-12-22 | 2015-04-22 | 北京大北农科技集团股份有限公司 | Application of insecticidal protein |
CN104744576A (en) * | 2015-04-16 | 2015-07-01 | 中国农业科学院植物保护研究所 | Bt (Bacillus thuringiensis) protein with insecticidal activity to lymantria dispar and application of Bt protein |
US20170029844A1 (en) * | 2015-07-23 | 2017-02-02 | Monsanto Technology Llc | Multi functional toxins |
CN109152347A (en) * | 2016-04-19 | 2019-01-04 | 美国陶氏益农公司 | For managing the combination of four kinds of VIP and CRY proteotoxins of the insect pest in plant |
US20180208631A1 (en) * | 2017-01-12 | 2018-07-26 | Monsanto Technology Llc | Pesticidal toxin proteins active against lepidopteran insects |
US20180291395A1 (en) * | 2017-04-03 | 2018-10-11 | Monsanto Technology Llc | Novel insect inhibitory proteins |
CN107383177A (en) * | 2017-08-16 | 2017-11-24 | 中国农业大学 | The artificial synthesized Bt killing genes mcry1Ab for transgenic anti-insect plants |
CN109971881A (en) * | 2019-04-11 | 2019-07-05 | 武汉大学 | A kind of primer pair and method for identifying multivalence transgenic pest-resistant rice genotype |
Non-Patent Citations (7)
Title |
---|
CAMILA SOARES FIGUEIREDO: "Synergism of the Bacillus thuringiensis Cry1, Cry2, and Vip3 Proteins in Spodoptera frugiperda Control", 《APPL BIOCHEM BIOTECHNOL》 * |
KARA L WELCH等: "Cross-resistance to toxins used in pyramided Bt crops and resistance to Bt sprays in Helicoverpa zea", 《J INVERTEBR PATHOL》 * |
傅强: "《远古的葱茏-古植物王国》", 31 March 2018 * |
冯学: "苏云金芽胞杆菌新基因的克隆及杀虫蛋白生物活性的研究", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
张丹丹等: "国产Bt-Cry1Ab和Bt-(Cry1Ab+Vip3Aa)玉米对草地贪夜蛾的抗性测定", 《植物保护》 * |
张杰等: "Bt杀虫基因专利保护现状与趋势", 《植物保护》 * |
黎裕等: "玉米转基因技术研发与应用现状及展望", 《玉米科学》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2022094790A1 (en) * | 2020-11-04 | 2022-05-12 | 中国农业大学 | Corn event 2a-7 and identification method therefor |
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