CN110981135A - Method for in-situ inhibition of release of hydrogen sulfide from tanning comprehensive sludge by microorganisms - Google Patents
Method for in-situ inhibition of release of hydrogen sulfide from tanning comprehensive sludge by microorganisms Download PDFInfo
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
Abstract
The invention discloses a method for in-situ inhibition of release of hydrogen sulfide in tanning comprehensive sludge by microorganisms, which comprises the following steps: taking sludge in a tannery sludge concentration tank as a sludge sample, and carrying out constant-temperature shaking culture on the sludge sample for 12-24 hours at the temperature of 25-30 ℃ and the rpm of 60-100 to obtain a microorganism enrichment culture; inoculating the microorganism enrichment culture into a sterilized screening culture medium for culture, and performing constant temperature shaking culture at 25-30 deg.C and 60-100rpm for 5-11 days; inoculating the cultured screening culture medium into a sterilized acclimation culture medium, and acclimating to obtain a functional microbial community culture; and finally, inoculating the functional microbial community culture into the tanning comprehensive sludge to inhibit the release of hydrogen sulfide. The method can obviously inhibit the release of hydrogen sulfide in the tannery sludge; simple operation, low cost and strong applicability.
Description
Technical Field
The invention relates to the technical field of tannery sludge treatment, in particular to a method for inhibiting release of hydrogen sulfide in tannery comprehensive sludge by microorganisms in situ.
Background
The tanning comprehensive sludge contains a large amount of sulfur-containing substances, and hydrogen sulfide is easily generated under the influence of microbial action and environmental conditions. Long-term exposure to such environments can cause a great deal of discomfort to the human body and even poisoning. Research shows that the release of hydrogen sulfide reaches release balance about 19 hours after the hydrogen sulfide is released from the tanning comprehensive sludge, namely the release of the hydrogen sulfide cannot be detected. Therefore, the research on the method for rapidly removing or inhibiting the release of the hydrogen sulfide is the key for solving the hydrogen sulfide pollution of the tanning wastewater treatment plant.
The hydrogen sulfide control technology of the prior tannery sewage treatment plant mainly comprises a physical method (dilution, masking, water washing and adsorption), a chemical method (absorption and oxidation) and a biological method (biological washing, a biological filter, biological trickling filtration and the like). Compared with physical methods and chemical methods, biological methods have the characteristics of low cost, environmental protection and the like, and are widely concerned.
However, the research of the biological method is mostly focused on the ectopic control, that is, the hydrogen sulfide gas is collected and then removed by the microorganism. Tannery sludge treatment facilities, especially tannery sludge concentration tank are large-scale open facilities, collect hydrogen sulfide and handle the back and need build collection system, and the input and maintenance cost is higher.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide a method for inhibiting the release of hydrogen sulfide in tannery comprehensive sludge in situ by microorganisms, which can obviously inhibit the release of hydrogen sulfide in tannery sludge; the operation is simple, the original sewage treatment facility is not required to be modified, and the cost is low; the method has strong applicability, and can obtain functional flora suitable for inhibiting the release of hydrogen sulfide in different tannery sludge pools, thereby achieving the effect of inhibiting the release of hydrogen sulfide.
In order to achieve the purpose, the invention is realized by adopting the following technical scheme.
A method for in-situ inhibition of release of hydrogen sulfide from tannery comprehensive sludge by microorganisms comprises the following steps:
step 1, preparing functional microbial flora
Taking sludge in a tannery sludge concentration tank as a sludge sample, and carrying out constant-temperature shaking culture on the sludge sample for 12-24 hours at the temperature of 25-30 ℃ and the rpm of 60-100 to obtain a microorganism enrichment culture; inoculating the microorganism enrichment culture into a sterilized screening culture medium for culture, and performing constant-temperature shaking culture at 25-30 ℃ and 60-100rpm for 5-11 days; inoculating the cultured screening culture medium into a sterilized acclimation culture medium, and acclimating to obtain a functional microbial community culture;
and 2, inoculating the functional microbial community culture into tanning comprehensive sludge to inhibit the release of hydrogen sulfide.
The technical scheme of the invention has the characteristics and further improvements that:
preferably, in step 1, before the sludge sample is subjected to the constant-temperature shaking culture, a nutrient substance is added to the sludge sample.
Further preferably, the nutrient comprises beef extract and peptone; wherein the concentration of the beef extract is 2-5g/L, and the concentration of the peptone is 4-10 g/L.
Further preferably, before the sludge sample is subjected to the constant temperature shaking culture, a phosphate buffer is further added to the sludge sample to maintain the pH of the sludge sample at 7.0-8.0 during the constant temperature shaking culture.
Preferably, in step 1, the inoculum size of the microorganism enrichment culture and the inoculum size of the screening medium after culture are respectively 7-10% of the volume of the sludge.
Preferably, in step 1, the selection medium is prepared by the following methodPreparing: performing thermal hydrolysis on the sludge in the tanning sludge concentration tank at the high temperature of 110-150 ℃ for 10-20min, then filtering by using filter paper, collecting filtrate, adding KH into the filtrate2PO42.0g/L、NH4Cl 0.4g/L、Na2CO30.4g/L、MgCl20.2g/L and Na2S10 g/L, and adjusting the pH to 7.0-8.0 to obtain the screening culture medium.
Preferably, in step 1, the acclimatization medium is prepared according to the following method: performing thermal hydrolysis on the sludge in the tanning sludge concentration tank at the high temperature of 110-150 ℃ for 10-20min, then filtering by using filter paper, collecting filtrate, adding KH into the filtrate2PO42.0g/L、NH4Cl 0.4g/L、Na2CO30.4g/L、MgCl20.2g/L、Na2S, adjusting the pH value to 7.0-8.0 to obtain a domestication culture medium; wherein, the Na2The concentration of S is 10-20 g/L.
Further preferably, the domestication method comprises:
inoculating the cultured screening medium to Na2Performing constant-temperature shake culture for 7-14 days at 25-30 ℃ and 60-100rpm in an acclimatization culture medium with the S concentration being a first concentration gradient; after the culture was completed, it was re-inoculated into Na2Performing constant-temperature shake culture at 25-30 deg.C and 60-100rpm in acclimatization culture medium with S concentration of second concentration gradient for 7-14 days; circulating in the same way until the temperature is Na2Culturing in acclimatization culture medium with S concentration of Nth concentration gradient for 7-14 days; the first concentration gradient is not less than 10g/L, and the Nth concentration gradient is 20 g/L.
Preferably, the first concentration gradient, the second concentration gradient and up to the Nth concentration gradient are equidistant concentration gradients or unequal concentration gradients.
Preferably, in the step 2, the inoculation amount of the functional microbial community culture is 10-15% of the volume of the tannery comprehensive sludge.
Preferably, in the step 2, when the functional microbial flora culture is inoculated into the tannery integrated sludge, the sludge is in a stirring state or a static state.
Compared with the prior art, the invention has the beneficial effects that:
1. the inhibition speed is high and the effect is obvious. The method provided by the invention is adopted to treat the tannery sludge, the removal rate of sulfur ions in the sludge after 1 hour can reach more than 70%, the release of hydrogen sulfide can not be detected within 2 hours, and the inhibition effect is obvious.
2. The applicability is strong. The method provided by the invention can be used for obtaining the functional flora suitable for inhibiting the release of the hydrogen sulfide in the tannery wastewater treatment plants, so that the effect of inhibiting the release of the hydrogen sulfide is achieved.
3. Reduce the treatment cost of tannery sludge odor. The invention can achieve the purpose of inhibiting the release of hydrogen sulfide odor by directly adding the microbial flora into the tannery sludge, has simple operation, does not need to modify the original sewage treatment facility, and obviously reduces the odor treatment cost.
4. The post-treatment performance of the sludge is improved. The dry weight of the sludge treated by the microbial flora obtained by the method is not increased but reduced, and the filtering performance is obviously improved, thereby being beneficial to the subsequent treatment of the sludge.
5. Is green and environment-friendly. The method provided by the invention does not need to add any substances harmful to the environment, thereby avoiding the generation of secondary pollution.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a photomicrograph of a functional microbial community obtained by the method for in-situ inhibition of release of hydrogen sulfide from tannery comprehensive sludge by microorganisms provided by the invention;
FIG. 2 is a comparison graph of sludge before and after tannery comprehensive sludge treatment by using the method for inhibiting hydrogen sulfide release of tannery comprehensive sludge in situ by using microorganisms provided by the invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a method for inhibiting release of hydrogen sulfide in tannery comprehensive sludge by microorganisms in situ, which comprises the following steps:
step 1, preparing functional microbial flora
Taking sludge in a tannery sludge concentration tank as a sludge sample, and carrying out constant-temperature shaking culture on the sludge sample for 12-24 hours at the temperature of 25-30 ℃ and the rpm of 60-100 to obtain a microorganism enrichment culture; inoculating the microorganism enrichment culture into a sterilized screening culture medium for culture, and performing constant-temperature shaking culture at 25-30 ℃ and 60-100rpm for 5-11 days; inoculating the cultured screening culture medium into a sterilized acclimation culture medium, and acclimating to obtain a functional microbial community culture;
and 2, inoculating the functional microbial flora into the tanning comprehensive sludge to inhibit the release of hydrogen sulfide.
The principle of the method is as follows:
the method provided by the invention takes tannery comprehensive sludge as a strain source, and the functional microbial flora prepared by enrichment, screening and domestication contains chemoautotrophic and heterotrophic bacteria which can obtain energy from sulfur-containing organic matters and inorganic matters in the sludge and maintain the self growth; at the same time, the sulfur-containing compound is oxidized to high-priced sulfur, thereby suppressing the release of hydrogen sulfide.
The equation specifically involved is as follows:
H2S+0.5O2→S0+H2O(1)
in particular, the method provided by the present invention is further described by the following examples.
Example 1
Step 1, preparing functional microbial flora
① adding nutrient substances including beef extract and peptone into 0.1L of sludge in a leather-making sludge concentration tank of a certain tannery, wherein the beef extract is added according to the concentration of 2g/L, and the peptone is added according to the amount of 4g/L, and performing shake culture at constant temperature of 30 ℃ and 100rpm for 24 hours to obtain the microorganism-enriched culture.
② the microorganism enrichment culture is inoculated into the sterilized screening culture medium according to the inoculation amount of 10 percent for culture, and the culture medium turns turbid after being shake cultured for 11 days at the constant temperature of 30 ℃ and 80 rpm.
Wherein, the screening culture medium is prepared according to the following method: thermally hydrolyzing the sludge in the tanning sludge concentration tank at 150 ℃ for 10min, filtering with filter paper, collecting filtrate, and adding KH into the filtrate2PO42.0g/L、NH4Cl 0.4g/L、Na2CO30.4g/L、MgCl20.2g/L and Na2S10 g/L, and adjusting the pH to 7.5 to obtain the screening culture medium.
③ inoculating the turbid screening culture medium into sterilized acclimation culture medium according to the inoculation amount of 10%, and acclimatizing to obtain functional microbial flora culture.
The domestication culture medium is prepared according to the following method: thermally hydrolyzing the sludge in the tanning sludge concentration tank at 150 ℃ for 10min, filtering with filter paper, collecting filtrate, and adding KH into the filtrate2PO42.0g/L、NH4Cl 0.4g/L、Na2CO30.4g/L、MgCl20.2g/L、Na2S12g/L, and adjusting the pH value to 7.5 to obtain the domestication culture medium.
The specific domestication method comprises the following steps: will be provided withThe turbid screening medium was inoculated with Na2Carrying out constant-temperature shaking culture for 14 days in an acclimatization culture medium with the S concentration of 12g/L under the conditions of 25 ℃ and 80 rpm; after the culture was completed, it was re-inoculated into Na2Performing shake culture at constant temperature of 25 ℃ and 80rpm in an acclimatization culture medium with the S concentration of 14g/L for 14 days; circulating in a concentration gradient of 2g/L until the concentration is higher than Na2Culturing in 20g/L acclimatization culture medium for 14 days.
And 2, inoculating the functional microbial flora culture obtained in the step 1 into a tanning sludge concentration tank of the tannery according to the inoculation amount of 10% of the sludge volume in the tanning sludge concentration tank of the tannery, stirring, and detecting the release condition of hydrogen sulfide. According to the determination, the content of the sulfur ions in the sludge is reduced by 72.02 percent after stirring for 1 hour, and the release of the hydrogen sulfide can not be detected after 1 hour and 30 minutes.
Example 2
Step 1, preparing functional microbial flora
① adding nutrient substances including beef extract and peptone into 0.1L of sludge in a leather-making sludge concentration tank of a certain tannery, wherein the beef extract is added according to the concentration of 5g/L, and the peptone is added according to the amount of 10g/L, and performing shake culture at constant temperature of 25 ℃ and 60rpm for 20 hours to obtain the microorganism-enriched culture.
② the microorganism enrichment culture is inoculated into the sterilized screening culture medium according to the inoculation amount of 10 percent for culture, and the culture medium turns turbid after shaking culture at the constant temperature of 30 ℃ and 60rpm for 8 days.
Wherein, the screening culture medium is prepared according to the following method: thermally hydrolyzing the sludge in the tanning sludge concentration tank at 110 ℃ for 15min, filtering with filter paper, collecting filtrate, and adding KH into the filtrate2PO42.0g/L、NH4Cl 0.4g/L、Na2CO30.4g/L、MgCl20.2g/L and Na2S10 g/L, and adjusting the pH to 7.0 to obtain the screening culture medium.
③ inoculating the turbid screening culture medium into sterilized acclimation culture medium according to the inoculation amount of 7%, and acclimatizing to obtain functional microbial community culture.
The domestication culture medium is prepared according to the following method: thermally hydrolyzing the sludge in the tanning sludge concentration tank at 110 ℃ for 15min, filtering with filter paper, collecting filtrate, and adding KH into the filtrate2PO42.0g/L、NH4Cl 0.4g/L、Na2CO30.4g/L、MgCl20.2g/L、Na2S15 g/L, and adjusting the pH to 7.0 to obtain the domestication culture medium.
The specific domestication method comprises the following steps: inoculating the turbid screening Medium to Na2Carrying out shake culture at constant temperature of 25 ℃ and 80rpm for 10 days in an acclimatization culture medium with the S concentration of 15 g/L; after the culture was completed, it was re-inoculated into Na2S concentration is 20g/L, and then the culture is performed with constant temperature shaking for 10 days under the conditions of 25 ℃ and 80 rpm.
And 2, inoculating the functional microbial flora culture obtained in the step 1 into a tanning sludge concentration tank of the tanning factory according to the inoculation amount of 10%, stirring, and detecting the release condition of hydrogen sulfide. According to the measurement, the content of the sulfur ions in the sludge is reduced by 81.30% after stirring for 1 hour, and the release of the hydrogen sulfide can not be detected after 1 hour and 15 minutes.
Example 3
Step 1, preparing functional microbial flora
① adding nutrient substances including beef extract and peptone into 0.1L of sludge in a leather-making sludge concentration tank of a certain tannery, wherein the beef extract is added according to the concentration of 3.5g/L, and the peptone is added according to the amount of 7g/L, and performing shake culture at constant temperature of 30 ℃ and 100rpm for 12 hours to obtain the microorganism-enriched culture.
② the microorganism enrichment culture is inoculated into the sterilized screening culture medium according to the inoculation amount of 10 percent for culture, and the culture medium turns turbid after being shake cultured for 5 days at the constant temperature of 30 ℃ and 80 rpm.
Wherein, the screening culture medium is prepared according to the following method: thermally hydrolyzing the sludge in the tanning sludge concentration tank at 120 ℃ for 10min, filtering with filter paper, collecting filtrate, and adding KH into the filtrate2PO42.0g/L、NH4Cl 0.4g/L、Na2CO30.4g/L、MgCl20.2g/L and Na2S10 g/L, and adjusting the pH to 8.0 to obtain the screening culture medium.
③ inoculating the turbid screening culture medium into sterilized acclimation culture medium according to the inoculation amount of 10%, and acclimatizing to obtain functional microbial flora culture.
The domestication culture medium is prepared according to the following method: thermally hydrolyzing the sludge in the tanning sludge concentration tank at 120 ℃ for 10min, filtering with filter paper, collecting filtrate, and adding KH into the filtrate2PO42.0g/L、NH4Cl 0.4g/L、Na2CO30.4g/L、MgCl20.2g/L、Na2S12g/L, and adjusting the pH to 8.0 to obtain the domestication culture medium.
The specific domestication method comprises the following steps: inoculating the turbid screening Medium to Na2Carrying out constant-temperature shaking culture for 14 days in an acclimatization culture medium with the S concentration of 12g/L under the conditions of 25 ℃ and 80 rpm; after the culture was completed, it was re-inoculated into Na2Performing shake culture at constant temperature of 25 ℃ and 80rpm in an acclimatization culture medium with the S concentration of 15g/L for 14 days; after the culture was completed, it was re-inoculated into Na2Performing shake culture at constant temperature of 25 ℃ and 80rpm in an acclimatization culture medium with the S concentration of 18g/L for 14 days; after the culture was completed, it was re-inoculated into Na2S concentration of 20g/L, and then shaking culture at constant temperature of 25 ℃ and 80rpm for 14 days.
And 2, inoculating the functional microbial flora culture obtained in the step 1 into a tanning sludge concentration tank of the tanning factory according to the inoculation amount of 15%, stirring, and detecting the release condition of hydrogen sulfide. It was determined that stirring for 1 hour reduced the sulfide ion content of the sludge by 85.56%, and no hydrogen sulfide release was detected after 1 hour and 10 minutes.
Example 4
Step 1, preparing functional microbial flora
① adding nutrient substances including beef extract and peptone into 0.1L of sludge in a leather-making sludge concentration tank of a certain tannery, wherein the beef extract is added according to the concentration of 2g/L, the peptone is added according to the dosage of 10g/L, then phosphate buffer solution is added to maintain the pH value of the sludge sample to be 7.0-8.0 in the process of constant-temperature shaking culture, and then the sludge sample is subjected to constant-temperature shaking culture at the temperature of 30 ℃ and 100rpm for 12 hours to obtain the microorganism enriched culture.
② the microorganism enrichment culture is inoculated into the sterilized screening culture medium according to the inoculation amount of 10 percent for culture, and the culture medium turns turbid after being shake cultured for 11 days at the constant temperature of 30 ℃ and 80 rpm.
Wherein, the screening culture medium is prepared according to the following method: thermally hydrolyzing the sludge in the tanning sludge concentration tank at 150 ℃ for 10min, filtering with filter paper, collecting filtrate, and adding KH into the filtrate2PO42.0g/L、NH4Cl 0.4g/L、Na2CO30.4g/L、MgCl20.2g/L and Na2S10 g/L, and adjusting the pH to 7.5 to obtain the screening culture medium.
③ inoculating the turbid screening culture medium into sterilized acclimation culture medium according to the inoculation amount of 10%, and acclimatizing to obtain functional microbial flora culture.
The domestication culture medium is prepared according to the following method: thermally hydrolyzing the sludge in the tanning sludge concentration tank at 150 ℃ for 10min, filtering with filter paper, collecting filtrate, and adding KH into the filtrate2PO42.0g/L、NH4Cl 0.4g/L、Na2CO30.4g/L、MgCl20.2g/L、Na2S12g/L, and adjusting the pH to 7.5 to obtain the domestication culture medium.
The specific domestication method comprises the following steps: inoculating the turbid screening Medium to Na2Carrying out constant-temperature shaking culture for 14 days in an acclimatization culture medium with the S concentration of 12g/L under the conditions of 25 ℃ and 80 rpm; after the culture was completed, it was re-inoculated into Na2Performing shake culture at constant temperature of 25 ℃ and 80rpm in an acclimatization culture medium with the S concentration of 14g/L for 14 days; circulating in a concentration gradient of 2g/L until the concentration is higher than Na2Culturing in 20g/L acclimatization culture medium for 14 days.
And 2, inoculating the functional microbial flora culture obtained in the step 1 into a tanning sludge concentration tank of the tanning factory according to the inoculation amount of 15%, standing, and detecting the release condition of hydrogen sulfide. Through measurement, the content of the sulfur ions in the sludge is reduced by 78.20 percent after stirring for 1 hour, and the release of the hydrogen sulfide can not be detected after 1 hour and 20 minutes.
In the above examples, the filter paper was medium speed filter paper with a pore size of 30-50 microns when preparing the screening medium and the acclimatization medium.
In the above examples, liquid preservation was employed after obtaining the functional microbial flora culture, (1) short-term preservation: 0.5-1mL of domesticated functional flora culture is put in a strain storage tube and stored in a refrigerator at 4 ℃ for 1 month. (2) And (3) long-term preservation: 0.5-1mL of the domesticated functional flora culture is put into a strain storage tube and stored at-20 ℃ or-80 ℃ for 1 year or 2 years. The restored strain can be directly used for enlarged culture after rejuvenation, thus avoiding the troubles of re-enrichment, screening and domestication.
In the above examples, after obtaining the functional microbial flora culture, it is necessary to perform the scale-up culture to satisfy the treatment of the total amount of sludge in the tanning sludge concentration tank of the tannery. Firstly, preparing an expansion culture medium, and preparing according to a screening culture medium formula; then inoculating the culture of the functional microbial flora obtained after domestication into an amplification culture medium (the inoculation amount is 7-10% of the volume of the amplification culture medium), performing amplification culture, and using the culture after the amplification culture medium becomes turbid.
Further, in the above-mentioned case,
the functional microbial flora prepared in example 1 was observed with a microscope, and as a result, as shown in fig. 1, it was found by the microscope observation that the functional microbial flora obtained by screening was free to swim under the microscope observation, and therefore, the microbial flora was added to the stationary sludge, and the microbial flora was also self-dispersed in the sludge, and the hydrogen sulfide release was suppressed. But the experimental effect shows that the dispersion of the flora is more uniform and the action effect is better when the sludge is in a stirring state.
FIG. 2 is a photograph comparing the tannery complex sludge before and after the treatment of example 1; wherein, the upper row is a photo picture of the leather-making comprehensive sludge which is not treated, and the lower row is a photo picture of the leather-making comprehensive sludge which is treated by the example 1.
Referring to fig. 2, it can be seen that the dry weight of the sludge treated with the microbial flora obtained in example 1 is not increased but rather reduced compared to untreated tannery complex sludge. The degradation of microbial flora reduces insoluble solid content in sludge, reduces volume and loosens structure, thus improving the filtering performance of sludge and being beneficial to the subsequent treatment of sludge.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
Claims (10)
1. A method for in-situ inhibition of release of hydrogen sulfide from tannery comprehensive sludge by microorganisms is characterized by comprising the following steps:
step 1, preparing functional microbial flora
Taking sludge in a tannery sludge concentration tank as a sludge sample, and carrying out constant-temperature shaking culture on the sludge sample for 12-24 hours at the temperature of 25-30 ℃ and the rpm of 60-100 to obtain a microorganism enrichment culture; inoculating the microorganism enrichment culture into a sterilized screening culture medium for culture, and performing constant-temperature shaking culture at 25-30 ℃ and 60-100rpm for 5-11 days; inoculating the cultured screening culture medium into a sterilized acclimation culture medium, and acclimating to obtain a functional microbial community culture;
and 2, inoculating the functional microbial community culture into tanning comprehensive sludge to inhibit the release of hydrogen sulfide.
2. The method for the in-situ inhibition of the release of hydrogen sulfide from tannery integrated sludge by microorganisms according to claim 1, wherein in step 1, nutrient substances are added to the sludge sample before the sludge sample is subjected to the constant-temperature shaking culture.
3. The method for the microbial in-situ inhibition of the release of hydrogen sulfide from tannery integrated sludge according to claim 2, wherein the nutrients comprise beef extract and peptone; wherein the concentration of the beef extract is 2-5g/L, and the concentration of the peptone is 4-10 g/L.
4. The method for the in-situ inhibition of the release of hydrogen sulfide from tannery integrated sludge by microorganisms according to claim 3, wherein a phosphate buffer is further added to the sludge sample before the sludge sample is subjected to the constant temperature shaking culture so as to maintain the pH of the sludge sample to be 7.0-8.0 during the constant temperature shaking culture.
5. The method for the in-situ inhibition of the release of hydrogen sulfide from tannery integrated sludge by microorganisms according to claim 1, wherein in the step 1, the inoculation amount of the microorganism enrichment culture and the inoculation amount of the screening culture medium after the culture are respectively 7-10% of the volume of the sludge.
6. The method for the microorganism in-situ inhibition of the release of hydrogen sulfide from tannery integrated sludge according to claim 1, wherein in the step 1, the screening medium is prepared according to the following method: performing thermal hydrolysis on the sludge in the tanning sludge concentration tank at the high temperature of 110-150 ℃ for 10-20min, then filtering by using filter paper, collecting filtrate, adding KH into the filtrate2PO42.0g/L、NH4Cl 0.4g/L、Na2CO30.4g/L、MgCl20.2g/L and Na2S10 g/L, and adjusting the pH to 7.0-8.0 to obtain the screening culture medium.
7. The method for the microorganism in-situ inhibition of the release of hydrogen sulfide from tannery integrated sludge according to claim 1, wherein in the step 1, the acclimation culture medium is prepared according to the following method: performing thermal hydrolysis on the sludge in the tanning sludge concentration tank at the high temperature of 110-150 ℃ for 10-20min, then filtering by using filter paper, collecting filtrate, adding KH into the filtrate2PO42.0g/L、NH4Cl 0.4g/L、Na2CO30.4g/L、MgCl20.2g/L、Na2S, adjusting the pH value to 7.0-8.0 to obtain a domestication culture medium; wherein, the Na2The concentration of S is 10-20 g/L.
8. The method for the microorganism in-situ inhibition of the release of hydrogen sulfide from tannery integrated sludge according to claim 7, wherein the acclimation method comprises the following steps:
inoculating the cultured screening medium to Na2Performing constant-temperature shake culture for 7-14 days at 25-30 ℃ and 60-100rpm in an acclimatization culture medium with the S concentration being a first concentration gradient; after the culture was completed, it was re-inoculated into Na2Performing constant-temperature shake culture at 25-30 deg.C and 60-100rpm in acclimatization culture medium with S concentration of second concentration gradient for 7-14 days; circulating in the same way until the temperature is Na2Culturing in acclimatization culture medium with S concentration of Nth concentration gradient for 7-14 days; the first concentration gradient is not less than 10g/L, and the Nth concentration gradient is 20 g/L.
9. The method for the microbial in-situ inhibition of the release of hydrogen sulfide from tannery integrated sludge according to claim 8, wherein the first concentration gradient, the second concentration gradient and up to the Nth concentration gradient are equidistant concentration gradients or unequal concentration gradients.
10. The method for the microbial in-situ inhibition of the release of hydrogen sulfide from tannery integrated sludge according to claim 1, wherein in the step 2, the inoculation amount of the functional microbial flora culture is 10-15% of the volume of the tannery integrated sludge.
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| CN101200698A (en) * | 2007-11-26 | 2008-06-18 | 宁波工程学院 | Microorganism synchronously removing ammonia and sulfureted hydrogen foul gas and method for preparing the same |
| CN103184152A (en) * | 2011-12-30 | 2013-07-03 | 中国石油天然气股份有限公司 | Method for screening natural gas biological desulphurization flora |
| CN109260940A (en) * | 2018-09-28 | 2019-01-25 | 珠海中瑞环保科技有限公司 | A kind of complex microorganism deodorant and the preparation method and application thereof |
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| CN101200698A (en) * | 2007-11-26 | 2008-06-18 | 宁波工程学院 | Microorganism synchronously removing ammonia and sulfureted hydrogen foul gas and method for preparing the same |
| CN103184152A (en) * | 2011-12-30 | 2013-07-03 | 中国石油天然气股份有限公司 | Method for screening natural gas biological desulphurization flora |
| CN109260940A (en) * | 2018-09-28 | 2019-01-25 | 珠海中瑞环保科技有限公司 | A kind of complex microorganism deodorant and the preparation method and application thereof |
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