CN113118206A - Microbial remediation method for heavy metal contaminated soil of ex-service slag field of smelting plant - Google Patents
Microbial remediation method for heavy metal contaminated soil of ex-service slag field of smelting plant Download PDFInfo
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- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 5
- 239000012881 co-culture medium Substances 0.000 claims description 4
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- CYDQOEWLBCCFJZ-UHFFFAOYSA-N 4-(4-fluorophenyl)oxane-4-carboxylic acid Chemical compound C=1C=C(F)C=CC=1C1(C(=O)O)CCOCC1 CYDQOEWLBCCFJZ-UHFFFAOYSA-N 0.000 claims description 3
- 241001147780 Alicyclobacillus Species 0.000 claims description 3
- 241000862484 Alicyclobacillus sp. Species 0.000 claims description 3
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 3
- 239000007832 Na2SO4 Substances 0.000 claims description 3
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- VCJMYUPGQJHHFU-UHFFFAOYSA-N iron(III) nitrate Inorganic materials [Fe+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O VCJMYUPGQJHHFU-UHFFFAOYSA-N 0.000 claims description 3
- HWSZZLVAJGOAAY-UHFFFAOYSA-L lead(II) chloride Chemical compound Cl[Pb]Cl HWSZZLVAJGOAAY-UHFFFAOYSA-L 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 238000009629 microbiological culture Methods 0.000 claims description 3
- KMUONIBRACKNSN-UHFFFAOYSA-N potassium dichromate Chemical compound [K+].[K+].[O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O KMUONIBRACKNSN-UHFFFAOYSA-N 0.000 claims description 3
- 239000001540 sodium lactate Substances 0.000 claims description 3
- 229940005581 sodium lactate Drugs 0.000 claims description 3
- 235000011088 sodium lactate Nutrition 0.000 claims description 3
- 229910052938 sodium sulfate Inorganic materials 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 241001659802 uncultured sulfate-reducing bacterium Species 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 2
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 2
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 2
- 239000011686 zinc sulphate Substances 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 claims 2
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- 238000001514 detection method Methods 0.000 abstract description 4
- 230000007935 neutral effect Effects 0.000 abstract 1
- 239000011701 zinc Substances 0.000 description 19
- 239000011651 chromium Substances 0.000 description 13
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 10
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 10
- 229910052725 zinc Inorganic materials 0.000 description 10
- 229910052804 chromium Inorganic materials 0.000 description 9
- 239000011133 lead Substances 0.000 description 9
- 241000196324 Embryophyta Species 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 7
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- 230000008439 repair process Effects 0.000 description 6
- 238000011065 in-situ storage Methods 0.000 description 5
- JQJCSZOEVBFDKO-UHFFFAOYSA-N lead zinc Chemical compound [Zn].[Pb] JQJCSZOEVBFDKO-UHFFFAOYSA-N 0.000 description 4
- VQWFNAGFNGABOH-UHFFFAOYSA-K chromium(iii) hydroxide Chemical group [OH-].[OH-].[OH-].[Cr+3] VQWFNAGFNGABOH-UHFFFAOYSA-K 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
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- 238000000635 electron micrograph Methods 0.000 description 2
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 2
- 229910052981 lead sulfide Inorganic materials 0.000 description 2
- 229940056932 lead sulfide Drugs 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
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- 229910052984 zinc sulfide Inorganic materials 0.000 description 2
- DRDVZXDWVBGGMH-UHFFFAOYSA-N zinc;sulfide Chemical compound [S-2].[Zn+2] DRDVZXDWVBGGMH-UHFFFAOYSA-N 0.000 description 2
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- 229910052793 cadmium Inorganic materials 0.000 description 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
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- 239000003673 groundwater Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
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- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
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Images
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention provides a microbial remediation method for heavy metal contaminated soil in a retired slag yard of a smelting plant, which selects sulfate reducing bacteria and iron reducing bacteria mixed bacteria under a neutral condition and contains Zn2+，Pb2+、Cr6+Until Zn is contained in the medium2+，Pb2+、Cr6+When the concentration is lower than the detection line, selecting the strain in the bottle for switching, and gradually increasing Zn2+，Pb2+、Cr6+Performing gradient domestication on the concentration; adding the domesticated bacterial liquid into the polluted soil, wherein the addition amount of the bacterial liquid is 1:1-3:1 in terms of the mass-to-volume ratio (g: mL) of the soil to the bacterial liquid, culturing for 4-7 days at 30-35 ℃, and then repairing soluble Zn in the soil2+，Pb2+、Cr6+Less than 0-0.05mg/L, 0-0.05mg/L and 0-0.05mg/L, and meets the III-class water standard of underground water quality standard.
Description
Technical Field
The invention belongs to the technical field of microorganisms, relates to a contaminated soil remediation method, and particularly relates to a microbial remediation method technology for heavy metal contaminated (zinc, lead and chromium exceed the standards) soil in a retired slag yard of a smelting plant.
Background
At present, China is seriously polluted by heavy metals such as zinc, lead, cadmium and the like, more than 40 percent of the heavy metal pollution is from the mining, dressing and smelting industry of nonferrous metals, and the peripheral environment pollution is particularly obvious due to long-term unorganized stacking and substandard discharge of lead-zinc smelting slag. Heavy metals in the smelting slag not only have the characteristics of wide diffusion, high toxicity, difficult treatment and the like, but also destroy the structure of an endogenous microbial community, and the derived food safety problem caused by the heavy metals migrating to the surrounding soil is more directly harmful to the health of human beings. Therefore, the repair and treatment of the lead-zinc smelting slag field become problems to be solved urgently at present. At present, smelting slag treatment methods are numerous, and most of the smelting slag treatment methods adopt a chemical treatment technology of adding a reducing agent, but the method has high operation cost, and the generated heavy metal sludge is easy to dissolve again to cause secondary pollution. The application of the microbial remediation technology to the remediation of heavy metal pollution is a hotspot of research in recent years, and the capability of adsorbing or converting a heavy metal binding state of some functional microorganisms is utilized to convert the microorganisms from a dissolved state to a stable state, so that the toxicity and the mobility of heavy metals are weakened, the bioavailability is limited, and the risk of environmental pollution is reduced.
Disclosure of Invention
In order to solve the problems, the invention aims to provide a composite microorganism for repairing polluted soil, which can cure over-standard zinc, lead and chromium in a ex-service slag field of a smelting plant.
Another object of the present invention is to provide a co-culture medium for the above complex microorganism.
The third purpose of the invention is to provide a microbial remediation method technology for heavy metal contaminated soil in a retired slag yard of a smelting plant, which realizes long-acting stable solidification of heavy metals zinc, lead and chromium in the slag yard by the reduction and regulation of microbes and by increasing the pH and reducing the Eh.
In order to achieve the aim, the invention provides a compound microorganism for repairing polluted soil, which is a mixed culture of sulfate reducing bacteria and iron reducing bacteria in logarithmic phase according to the bacteria liquid volume ratio of 1: 1;
wherein, the classification name of the sulfate reducing bacteria is as follows: uncultured sulfate-reducing bacterium SRB-4, the preservation unit is: china center for type culture Collection, Address: the preservation date of Wuhan university in Wuhan, China is as follows: 9/14/2015, with a deposition number: CCTCC NO: m2015535;
the classification names of the iron reducing bacteria are: alicyclobacillus (Alicyclobacillus sp.) biomeek-B, depository: china general microbiological culture Collection center, address: the microbial research institute of the Chinese academy of sciences has the following preservation date: 12/26/2012 with the deposit number: CGMCC NO. 7039.
The sulfate reducing bacteria can reduce sulfate ions in the environment into S2-The heavy metal exists in a sulfide form and is fixed; the iron reducing bacteria can regulate Eh in a system and increase the repair effectiveness of the sulfate reducing bacteria.
The invention also provides a co-culture medium for amplifying and culturing the compound microorganism for repairing the polluted soil, and the preparation method of the culture medium comprises the following steps: sodium lactate 0-3.5g, NH4Cl 0-1.0g，Na2SO4 0-1.0g、MgSO40-0.1g、K2HPO4 0-0.5g，(NH4)2Fe(SO4)2·6H2O0-0.65 g and CaCl20-0.1g of a solution of 1mol/L H in 1L of distilled water2SO4Or NaOH is used for adjusting the pH value to 7.0-7.2; then chemically pure ZnSO4、PbCl2、K2Cr2O7、Fe(NO3)3Adding the solid into 1L culture medium, and adjusting Zn2+Concentration of 0.5g/L, Pb2+The concentration is 0.5g/L, Cr6+Concentration of 20mg/L, Fe3+The concentration is 2-3 g/L.
The invention also provides a microbial remediation method technology for heavy metal contaminated soil in the ex-service slag yard of the smelting plant, which comprises the following steps:
1) use the above forThe composite microorganism for repairing the polluted soil is inoculated in the culture medium and is placed at the temperature of between 30 and 35 ℃ for 150--1The air bath constant temperature oscillator is used for culturing for 3-4 days until the bacterial concentration of the bacterial liquid is 8.3 multiplied by 107～9.8×107Per mL;
2) for the compound microorganism cultured in the step 1), Zn is increased by adopting gradient2+，Pb2+、Cr6+Acclimating to obtain highly-tolerant heavy metal-tolerant composite microorganism bacterial liquid with bacterial concentration of 8.2 × 107-9.6×107Per mL;
3) inoculating the bacterial liquid cultured in the step 2) into the polluted soil, and culturing for 4-7 days at the temperature of 30-35 ℃, so that the soil is repaired.
Separated sampling and detection of Zn2+，Pb2+、Cr6+，Fe3+And (4) concentration.
Further, the inoculation volume of the strain in the step 1) is 10-15% of the volume of the culture medium, and the bacterial concentration of the compound microorganism initially supplied for inoculation is 7.5 multiplied by 107-8.8×107one/mL.
Further, the domestication method in the step 2) comprises the following steps: the inoculation volume of each composite microorganism is 8-12% of the volume of the culture medium, and the composite microorganism is placed at the temperature of 30-35 ℃ for 150-160 r.min-1The air bath constant temperature oscillator is used for culturing for 3 to 4 days, and Zn is contained in the culture medium2+，Pb2+、Cr6+Gradually increasing the concentration to Zn according to the concentration gradients of 200mg/L, 200mg/L and 20mg/L respectively2+The concentration reaches 2.2g/L, Pb2+The concentration reaches 1.8g/L, Cr6+The concentration reaches 100mg/L, and the bacterial concentration of the compound microorganism reaches 7.0 multiplied by 10 in each culture7～8.0×107And performing next-stage domestication culture on the cells/mL.
The tolerance of the composite bacteria to heavy metals is improved through gradual domestication, and a culture medium is periodically added in the culture process to supplement the volatilization of the solution in the reaction process.
Furthermore, the adding amount of the bacterial liquid in the step 3) is that the mass-volume ratio g: mL of the soil to the bacterial liquid is 1:1-3: 1.
Cultivation of Zn by acclimatization2+Has a tolerance concentration of 05g/L to 2.2g/L, Pb2+The tolerance concentration of the strain is increased from 0.5g/L to 1.8g/L, Cr6+The tolerance concentration of the Fe-Fe alloy is increased from 20mg/L to 100mg/L3+The concentration is maintained at 2-3 g/L.
After the microorganism is adopted for remediation, soluble Zn in soil is remedied after soluble Zn in soil is remedied2+，Pb2+、Cr6+Less than 0-0.05mg/L, 0-0.05mg/L and 0-0.05mg/L, and the concentration of ferric iron is lower than that of the detection line, so that the standard of the second soil of the construction land is met. After the precipitate is detected, the main existence form of zinc is zinc sulfide, the main existence form of lead is lead sulfide, and the main existence form of chromium is chromium hydroxide precipitate, so that the precipitate is stable in soil, is not easy to migrate and has low bioavailability.
The invention has the beneficial effects that:
aiming at the characteristics of various types, wide distribution, difficult treatment and the like of heavy metal polluted by a smelting slag field, serious pollution to the underground water of peripheral soil and the like, the invention innovatively provides a microbial remediation method for restoring the polluted soil to realize long-acting stability of chromium.
Drawings
FIG. 1 is a scanning electron micrograph of sulfate-reducing bacteria used in the present invention.
FIG. 2 is a microscopic view of an iron-reducing bacterium used in the present invention.
Detailed Description
The present invention will be described in detail with reference to examples. The embodiments are for better understanding of the present invention and are not intended to limit the scope of the present invention, and any equivalent or known modifications are included in the scope of the present invention.
The content of zinc in the slag yard soil is 1.05-2.37%, the content of lead is 0.80-2.06%, and the content of Cr is 0.56-0.88%, and the content of the zinc is far beyond the soil pollution risk control standard (GB36600-2018) of soil environment quality construction land (the content of zinc, lead and chromium in the second-class land is respectively lower than 700, 800 and 5.7mg/kg), so the zinc, lead and chromium in the slag yard soil need to be treated to meet the soil environment quality standard.
The invention provides a compound microorganism for repairing polluted soil, which is all in logarithmic phase (10)7～108one/mL) of sulfate reducing bacteria and iron reducing bacteria according to the bacteria liquid volume ratio of 1: 1;
wherein, the classification name of the sulfate reducing bacteria is as follows: uncultured sulfate-reducing bacterium SRB-4, the preservation unit is: china center for type culture Collection, Address: in Wuhan university, the preservation date is: 9/14/2015, with a deposition number: CCTCC NO: m2015535, electron micrograph shown in FIG. 1.
The classification names of the iron reducing bacteria are: alicyclobacillus (Alicyclobacillus sp.) biomeek-B, depository: china general microbiological culture Collection center, address: the preservation date of the institute of microbiology of the Chinese academy of sciences is 2012, 12 and 26 days, and the preservation numbers are: CGMCC NO.7039, electron micrograph is shown in FIG. 2.
The formula of the co-culture medium of the compound microorganism is as follows: sodium lactate 1.3g, NH4Cl 0.5g，Na2SO4
0.5g、MgSO4 0.05g、K2HPO4 0.3g、(NH4)2Fe(SO4)2·6H2O0.45 g and CaCl20.05g of the product was dissolved in 1L of distilled water, the pH was adjusted to 7.0, and then chemically pure ZnSO was added4、PbCl2、K2Cr2O7、Fe(NO3)3Adding the solid into a culture medium, and preparing Zn2+Concentration of 0.5g/L, Pb2+The concentration is 0.5g/L, Cr6+Concentration of 20mg/L, Fe3+The concentration was 2.5 g/L.
A microbial remediation method for heavy metal contaminated soil of a smelting plant retired slag field comprises the following steps:
1) inoculating the composite microorganism for repairing the polluted soil into the culture medium, and placing at the temperature of 30-35 ℃ for 150-160 r.min-1Constant temperature vibration of air bathCulturing in oscillator for 3-4 days until the bacterial concentration is 8.3 × 107～9.8×107Per mL; the inoculation volume of the strain is 10-15% of the culture medium volume, and the bacterial concentration of the compound microorganism initially supplied for inoculation is 7.5 multiplied by 107-8.8×107one/mL.
2) For the compound microorganism cultured in the step 1), Zn is increased by adopting gradient2+，Pb2+、Cr6+Acclimating to obtain highly-tolerant heavy metal-tolerant composite microorganism bacterial liquid with bacterial concentration of 8.2 × 107-9.6×107Per mL;
the domestication method comprises the following steps: the inoculation volume of each composite microorganism is 8-12% of the volume of the culture medium, and the composite microorganism is placed at the temperature of 30-35 ℃ for 150-160 r.min-1The air bath constant temperature oscillator is used for culturing for 3 to 4 days, and Zn is contained in the culture medium2+，Pb2+、Cr6+Gradually increasing the concentration to Zn according to the concentration gradients of 200mg/L, 200mg/L and 20mg/L respectively2+The concentration reaches 2.2g/L, Pb2+The concentration reaches 1.8g/L, Cr6+The concentration reaches 100mg/L, and the bacterial concentration of the compound microorganism reaches 7.0 multiplied by 10 in each culture7～8.0×107And performing next-stage domestication culture on the cells/mL.
3) Inoculating the bacterial liquid cultured in the step 2) into the polluted soil, and culturing for 4-7 days at the temperature of 30-35 ℃, so that the soil is repaired. Wherein the addition amount of the bacterial liquid is 1:1-3:1 of the mass volume ratio g: mL of the soil to the bacterial liquid.
The proper working temperature of the bacterial liquid is 30-35 ℃, so that the season with the corresponding temperature is selected when in-situ remediation is carried out, and the remediation of soil pollutants is facilitated.
Example 1: lead-zinc tailing pond microorganism in-situ mineralization repair method
By adopting the microbial remediation technology, a certain lead-zinc tailing pond is taken as a main demonstration area for microbial in-situ mineralization remediation. The physicochemical properties of the tailings samples are shown in table 1.
TABLE 1 tailings sample physicochemical parameters
The tailings pond is close to desertification before restoration, and the water and soil loss is serious. The in-situ repair of microorganisms for 2 months is applied, the solidification of free heavy metals in the tailings is realized, the tailing reservoir area becomes bio-friendly, and a large amount of moss plants and a small amount of herbaceous plants begin to grow. After about 6 months, the plants are completely greened by adopting a microorganism in-situ restoration technology. Drilling 3-8m deep underground water monitoring wells in each area by using a drilling machine before repairing, and after repairing for 1 year, sampling and detecting results show that: from the aspect of monitoring the quality of the seepage water in the vertical pipe with the depth of 3-5m, the seepage water in the restoration area basically meets the requirements of class III water quality in the groundwater quality standard (GB 14848 and 2017).
Example 2 microbial remediation method for contaminated soil in wet detoxification chromium residue storage yard
And (3) taking 150g of the uniformly mixed samples in bottles, respectively adding 100mL of water, the culture medium and the mixed bacteria solution into an incubator, and standing for 3-4 days at normal temperature to determine the microbial remediation effect.
1) pH and Eh changes before and after remediation
The Eh of the repair group of the three samples added with the mixed bacteria liquid is reduced from the initial 260-300mV to-400 mV to-260 mV, the reduction amplitude is obvious, and the Eh of the blank control group tends to be stable (250mV to 310 mV); the pH of the repair group added with the mixed bacteria liquid is stabilized at 7.5-8.5, and the stable form of chromium in the pH and Eh ranges is Cr (OH)3。
2) Repairing effect of microorganism
Compared with blank control group data added with water and culture medium, the restoration group added with the mixed bacteria liquid has obvious restoration effect on chromium, the reduction fixation rate of Cr (VI) reaches more than 99.1 percent after 5-7 days of microbial restoration, and ferric iron is lower than the detection line. The precipitated product was detected as Cr (OH)3。
According to the method for repairing the microorganism in the soil polluted by the heavy metals in the slag yard, under the action of the sulfate reducing bacteria and the iron reducing bacteria, lead, zinc and chromium ions in the solution can form lead sulfide, zinc sulfide and trivalent chromium hydroxide precipitates, the precipitates are subjected to solid-liquid separation, and metabolites generated by the chromium hydroxide precipitates of the bacteria can chelate a small amount of free hexavalent chromium ions.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (6)
1. A composite microorganism for restoring polluted soil is characterized in that the composite microorganism is a mixed culture of sulfate reducing bacteria and iron reducing bacteria which are both in logarithmic phase according to the bacteria liquid volume ratio of 1: 1;
wherein, the classification name of the sulfate reducing bacteria is as follows: uncultured sulfate-reducing bacterium SRB-4, the preservation unit is: china center for type culture Collection, Address: the preservation date of Wuhan university in Wuhan, China is as follows: on 14 days 9/2015, the preservation number is: CCTCC NO: m2015535;
the classification names of the iron reducing bacteria are: alicyclobacillus (Alicyclobacillus sp.) biomeek-B, depository: china general microbiological culture Collection center, address: the microbial research institute of the Chinese academy of sciences has the following preservation date: 12/26/2012 with the deposit number: CGMCC NO. 7039.
2. A co-culture medium for culturing the complex microorganism according to claim 1 for remediating contaminated soil, which is prepared by: sodium lactate 0-3.5g, NH4Cl 0-1.0g，Na2SO4 0-1.0g、MgSO4 0-0.1g、K2HPO4 0-0.5g，(NH4)2Fe(SO4)2·6H2O0-0.65 g and CaCl20-0.1g of a solution of 1mol/L H in 1L of distilled water2SO4Or NaOH is used for adjusting the pH value to 7.0-7.2; then chemically pure ZnSO4、PbCl2、K2Cr2O7、Fe(NO3)3Adding the solid into 1L culture medium, and adjusting Zn2+Concentration of 0.5g/L, Pb2+At a concentration of 0.5g/L、Cr6+Concentration of 20mg/L, Fe3+The concentration is 2-3 g/L.
3. A microbial remediation method for heavy metal contaminated soil in a ex-service slag field of a smelting plant is characterized by comprising the following steps:
1) the composite microorganism for remediating contaminated soil as set forth in claim 1 is inoculated to the culture medium as set forth in claim 2, and the inoculated culture medium is placed at a temperature of between 30 and 35 ℃ for 150-160 r-min-1The air bath constant temperature oscillator is used for culturing for 3-4 days until the bacterial concentration of the bacterial liquid is 8.3 multiplied by 107～9.8×107Per mL;
2) for the compound microorganism cultured in the step 1), Zn is increased by adopting gradient2+，Pb2+、Cr6+Acclimating to obtain highly-tolerant heavy metal-tolerant composite microorganism bacterial liquid with bacterial concentration of 8.2 × 107-9.6×107Per mL;
3) inoculating the bacterial liquid cultured in the step 2) into the polluted soil, and culturing for 4-7 days at the temperature of 30-35 ℃, so that the soil is repaired.
4. The microbial remediation process of claim 3 wherein in step 1) the inoculum volume is from 10% to 15% of the culture volume and the initial inoculum combined microbial bacterial concentration is 7.5 x 107-8.8×107one/mL.
5. The microbial remediation method of claim 3 wherein the acclimatization method of step 2) comprises: the inoculation volume of each composite microorganism is 8-12% of the volume of the culture medium, and the composite microorganism is placed at the temperature of 30-35 ℃ for 150-160 r.min-1The air bath constant temperature oscillator is used for culturing for 3 to 4 days, and Zn is contained in the culture medium2+，Pb2+、Cr6+Gradually increasing the concentration to Zn according to the concentration gradients of 200mg/L, 200mg/L and 20mg/L respectively2+The concentration reaches 2.2g/L, Pb2+The concentration reaches 1.8g/L, Cr6+The concentration reaches 100mg/L, and the bacterial concentration of the compound microorganism reaches 7.0 multiplied by 10 in each culture7～8.0×107Per mL under the condition ofFirst-stage domestication and culture.
6. The microbial remediation method of claim 3 wherein the amount of bacterial liquid added in step 3) is such that the mass-to-volume ratio g: mL of soil to bacterial liquid is from 1:1 to 3: 1.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114606159A (en) * | 2022-03-02 | 2022-06-10 | 有研资源环境技术研究院(北京)有限公司 | Chromium reduction mixed bacteria and Fe synergy thereof3+Method for restoring chromium-polluted soil |
| CN114749479A (en) * | 2022-05-30 | 2022-07-15 | 中南大学 | Method for repairing arsenic-containing gold tailings by using plant-microorganism combination |
| CN114985447A (en) * | 2022-06-02 | 2022-09-02 | 中国地质大学（北京） | Microbial geochemical in-situ mineralization method for synchronously solidifying multiple heavy metals of nonferrous metal mine |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101602060A (en) * | 2009-07-03 | 2009-12-16 | 中南大学 | A kind of repairing method of microorganism in contaminated soil of chromium slag storage yard |
| CN105779325A (en) * | 2014-12-25 | 2016-07-20 | 北京有色金属研究总院 | Fatty acid bacillus and method for treatment of heavy metal pollution of mine by in situ mineralization of the same |
| CN106635854A (en) * | 2015-10-29 | 2017-05-10 | 北京有色金属研究总院 | Oligographic bacterium composition and application of oligographic bacterium composition in inhibition on generation of jarosite in biological leaching |
| CN106833674A (en) * | 2017-02-09 | 2017-06-13 | 东莞市环境科学研究所 | A kind of heavy-metal contaminated soil renovation agent preparation method |
| CN106868302A (en) * | 2015-12-11 | 2017-06-20 | 北京有色金属研究总院 | Sulfate reducing bacteria and its technique for reclaiming valuable metal in mine wastewater |
| CN107287129A (en) * | 2016-04-01 | 2017-10-24 | 兰州大学 | One plant can make heavy metal settle sulfate reducing bacteria and its application |
| CN109810930A (en) * | 2019-04-02 | 2019-05-28 | 王琳 | It is a kind of can restoration of soil polluted by heavy metal microbial bacterial agent and its preparation method and application |
| CN109967519A (en) * | 2017-12-28 | 2019-07-05 | 北京有色金属研究总院 | A kind of repairing method of microorganism of Tailings Dam heavy metal pollution |
-
2019
- 2019-12-31 CN CN201911410074.1A patent/CN113118206A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101602060A (en) * | 2009-07-03 | 2009-12-16 | 中南大学 | A kind of repairing method of microorganism in contaminated soil of chromium slag storage yard |
| CN105779325A (en) * | 2014-12-25 | 2016-07-20 | 北京有色金属研究总院 | Fatty acid bacillus and method for treatment of heavy metal pollution of mine by in situ mineralization of the same |
| CN106635854A (en) * | 2015-10-29 | 2017-05-10 | 北京有色金属研究总院 | Oligographic bacterium composition and application of oligographic bacterium composition in inhibition on generation of jarosite in biological leaching |
| CN106868302A (en) * | 2015-12-11 | 2017-06-20 | 北京有色金属研究总院 | Sulfate reducing bacteria and its technique for reclaiming valuable metal in mine wastewater |
| CN107287129A (en) * | 2016-04-01 | 2017-10-24 | 兰州大学 | One plant can make heavy metal settle sulfate reducing bacteria and its application |
| CN106833674A (en) * | 2017-02-09 | 2017-06-13 | 东莞市环境科学研究所 | A kind of heavy-metal contaminated soil renovation agent preparation method |
| CN109967519A (en) * | 2017-12-28 | 2019-07-05 | 北京有色金属研究总院 | A kind of repairing method of microorganism of Tailings Dam heavy metal pollution |
| CN109810930A (en) * | 2019-04-02 | 2019-05-28 | 王琳 | It is a kind of can restoration of soil polluted by heavy metal microbial bacterial agent and its preparation method and application |
Non-Patent Citations (1)
| Title |
|---|
| 孟春瑜: "Alicyclobacillus spp.应用于黄铜矿生物浸出的研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114606159A (en) * | 2022-03-02 | 2022-06-10 | 有研资源环境技术研究院(北京)有限公司 | Chromium reduction mixed bacteria and Fe synergy thereof3+Method for restoring chromium-polluted soil |
| CN114606159B (en) * | 2022-03-02 | 2023-06-20 | 有研资源环境技术研究院(北京)有限公司 | Chromium reduction mixed bacteria and synergistic Fe thereof 3+ Method for repairing chromium-polluted soil |
| CN114749479A (en) * | 2022-05-30 | 2022-07-15 | 中南大学 | Method for repairing arsenic-containing gold tailings by using plant-microorganism combination |
| CN114985447A (en) * | 2022-06-02 | 2022-09-02 | 中国地质大学（北京） | Microbial geochemical in-situ mineralization method for synchronously solidifying multiple heavy metals of nonferrous metal mine |
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