CN110963581A - Method for activating activity of biological membrane enzyme by combination of novel artificial substrate and plant - Google Patents
Method for activating activity of biological membrane enzyme by combination of novel artificial substrate and plant Download PDFInfo
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- CN110963581A CN110963581A CN201911282178.9A CN201911282178A CN110963581A CN 110963581 A CN110963581 A CN 110963581A CN 201911282178 A CN201911282178 A CN 201911282178A CN 110963581 A CN110963581 A CN 110963581A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
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Abstract
The invention discloses a method for activating the activity of a biological membrane dehydrogenase on the surface of an artificial substrate by combining a novel artificial substrate and a plant. The novel artificial matrix is formed by mutually overlapping polyvinyl chloride or polypropylene through hot melting of disordered filaments and then forming a three-dimensional net structure through die extrusion, so that the attachment area of microorganisms is increased, and the novel artificial matrix has the advantages of convenience in installation and removal and the like. The novel artificial substrate and the plant resurrection peanut are combined, and the self-cleaning capacity of the water body is improved through the interaction of the plant root system and the biological membrane on the surface of the substrate in the growth process of the resurrection peanut, so that the aim of optimizing the microenvironment of the water body is fulfilled, and the final aim of not only being environment-friendly but also continuously playing a role in repairing is fulfilled.
Description
Technical Field
The invention relates to the technical field of urban inland river water body pollution remediation, in particular to a method for replacing a traditional hardening process by a novel artificial matrix and plant combination and application thereof.
Background
The waterfront area is a transition area between land and a water area, is in interdependence and symbiotic relationship with cities, and has rich habitat, various species and ecological sensitivity. The traditional hard chemical engineering can not solve the fundamental problem, and simultaneously destroys the living environment, which is not beneficial to the virtuous circle of the river channel ecosystem.
The construction and the recovery of living environment are more and more important for realizing the sustainable development of urban waterfront space. Biofilms are polymers of autotrophic (algae, etc.) or heterotrophic microorganisms (viruses, bacteria, fungi, protozoa, etc.) that accumulate at solid-liquid junctions and are encapsulated by extracellular polymers with high water content. Soil microorganisms are the main source of soil enzymes, and soil enzyme activity is closely related to the structure and number of soil microorganism populations, so we reasonably believe that the enzyme activity of the biofilm is also mainly derived from its own microorganisms. The dehydrogenase activity of the microorganisms can decompose polluting organic matters in a water body into inorganic matters, is related to the removal of ammonia nitrogen, plays a role in transferring hydrogen in dehydrogenation reaction, reflects the activity state of organisms to a great extent, and can directly represent the strength of biological cells in degrading the substrates of the biological cells. Therefore, the enzyme activity can indicate the self-cleaning capacity of the water body to a certain extent.
In order to improve the dehydrogenase activity of the microorganisms, an ecosystem which is full of nutrients and more suitable for the environment needs to be provided for the growth and the propagation of the microorganisms, so that the aim of improving the microenvironment of a water body is fulfilled.
Disclosure of Invention
In order to improve the dehydrogenase activity of microorganisms to achieve the aim of improving the microenvironment of a water body, the invention provides a method for activating the activity of a biological membrane by combining a novel artificial substrate and plants, and the method can provide more nutrients and a more suitable living environment for the growth and the propagation of the microorganisms, thereby enhancing the activity of dehydrogenase.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a novel artificial substrate and plant combination method for activating the activity of biological membrane enzymes is characterized in that the novel artificial substrate is placed on the river bank, plants are planted on the upper surface of the novel artificial substrate, and the novel artificial substrate is used for repairing the urban inland river channel ecosystem through the interaction of plant root systems and biological membranes on the surface of the substrate.
The novel artificial substrate is preferably polyvinyl chloride or polypropylene which is overlapped with each other through hot melting of the broken filaments and then is extruded and formed into a three-dimensional net-shaped structure through a die, the three-dimensional net-shaped structure is a good ecological slope protection material, the three-dimensional net-shaped structure is easy to process into various shapes, and the attachment area of microorganisms is greatly increased.
The plant planted on the upper surface of the novel artificial substrate is preferably Thalia dealbata (Thalia dealbata Fraser) which belongs to the family Arundinaceae and belongs to perennial emergent aquatic herbaceous plants. During actual application, the novel artificial substrate is placed on the river bank, the upper surface of the novel artificial substrate is flush with the water surface, young plants of the ReLihua are planted on the upper surface of the novel artificial substrate, and the novel artificial substrate is fixed by a small amount of soil. The root system of the Thalia dealbata grows and spreads in the three-dimensional reticular structure of the novel artificial substrate, and forms a mutually beneficial and reciprocal relationship with the biological membrane on the novel artificial substrate, thereby being beneficial to the growth and the propagation of the biological membrane and the enhancement of the dehydrogenase activity of the biological membrane.
Compared with the prior art, the invention has the following beneficial effects:
the ecological system constructed by the novel artificial matrix combined plant can provide more nutrients and a more suitable living environment for the growth and the propagation of microorganisms, so that the activity of dehydrogenase of the ecological system is enhanced, the self-cleaning capacity of a river channel is macroscopically enhanced, and the microenvironment of a water body is improved.
The invention utilizes the novel artificial substrate which is built into a three-dimensional net structure by utilizing the three-dimensional random filaments of polyvinyl chloride or polypropylene, is a good ecological slope protection material, greatly increases the attachment area of microorganisms, and has the advantages of easy acquisition, easy processing into various shapes, low labor cost, convenient installation and removal and the like.
The novel artificial substrate and the plant resurrection peanut are combined, and the self-cleaning capacity of the water body is improved through the interaction of the plant root system and the biological membrane on the surface of the substrate in the growth process of the resurrection peanut, so that the aim of optimizing the microenvironment of the water body is fulfilled, and the final aim of not only being environment-friendly but also continuously playing a role in repairing is fulfilled.
Drawings
FIG. 1 is a photograph of the novel artificial substrate of the present invention;
FIG. 2 is a graph showing the activity changes of A and B substrate biofilm dehydrogenases at the same level in each season in the examples.
Detailed Description
For the purpose of facilitating an understanding of the present invention, the present invention will now be described by way of examples. It should be understood by those skilled in the art that the examples are only for the purpose of facilitating understanding of the present invention and should not be construed as specifically limiting the present invention.
In order to verify the activation effect of the novel artificial substrate and plant combination on the activity of the biological membrane enzyme, the following simulated ecological restoration test was carried out:
the novel artificial substrate is a three-dimensional reticular structure formed by mutually lapping polyvinyl chloride or polypropylene through hot melting of disordered filaments and then extruding and molding by a die, and the specification of the substrate used for the experiment is 100cm multiplied by 30cm multiplied by 100cm (shown in figure 1); the plant material is resfriction flower. The river in the schools of Wenzhou medical science and university is taken as an experimental river section, a novel artificial substrate A (flowers are planted above the novel artificial substrate A) and a novel artificial substrate B (flowers are not planted above the novel artificial substrate B) are placed in the same river section, and the upper surface of the substrate is flush with the water surface (the substrate is placed for about 15 months during the experiment).
The experiment characterizes the repair characteristics of the system by measuring the activity of the biofilm dehydrogenase. The biomembrane enzyme is mainly derived from dehydrogenase, and the biomembrane is separated from the surface of a novel artificial matrix placed at an experimental point.
(1) Sample collection and processing
The sampling method is to cut two small pieces of substrate with the specification of about 10cm multiplied by 30cm multiplied by 10cm at the position of 20cm and 50cm of A, B substrate respectively, and the small pieces are marked as A20、A50、B20、B50. The small substrate is put into a 500mL beaker, distilled water is used for washing off non-biological impurities attached to the surface of the small substrate, and about 300mL of distilled water is added, so that the surface biological membrane is peeled off by a physical method. After the ultrasonic treatment is carried out for 15min (ultrasonic frequency is 50Hz), the solution is transferred to a 1000mL conical flask, a proper amount of micro glass beads are added, and the mixture is fully shaken at room temperature for 15minAnd min, obtaining uniform biomembrane mixed liquor, and using the mixed liquor as a subsequent experimental sample for later use.
(2) Determination of biofilm dehydrogenase Activity
And (3) preparing a standard curve: diluting the standard solution with the concentration of 1 g/LTTC-glucose by 100 times, respectively taking 0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 and 1.4mL of the solution, sequentially adding 2mL of Tris-HCI buffer solution and 1mL of 10% Na2S new prepared solution, and shaking uniformly; after the solution is fully developed, accurately adding 5mL of toluene solution, completely shaking and uniformly mixing, and completely extracting TF. Standing for a while, and transferring to a 1cm cuvette for stabilization after the solution is layered. And (3) taking the organic solution layer, measuring the corresponding absorbance (A) value at the wavelength of 485nm by using an 752 ultraviolet spectrophotometer, plotting the dehydrogenase activity, and drawing a standard curve by using a reagent blank as a control.
The sample was assayed using the TTC-dehydrogenase assay, as follows:
10mL of the biomembrane preparation solution is put in a 15mL centrifuge tube, centrifuged at 4000rpm/min for 5min, sequentially added with 2mL of each of Tris-HCl buffer solution, 0.1mol/L glucose solution and 0.5% TTC, put in a constant-temperature water bath kettle at 37 ℃ for culture for 6h, taken out, added with 2 drops of concentrated sulfuric acid to terminate the reaction, accurately added with 5mL of toluene, shaken well, centrifuged at 4000rpm/min for 5min, and taken out to be subjected to color comparison. Under the above conditions, the amount of 1/μ g TF produced in 1 hour was 1 enzyme activity unit.
(3) Comparison of measurement results
As shown in Table 1, A, B results of overall differential analysis of the activity of the biomembrane dehydrogenase of the substrate A and the biomembrane dehydrogenase of the substrate B show that the difference between the activity of the biomembrane dehydrogenase of the substrate A and the activity of the biomembrane dehydrogenase of the substrate B is statistically significant (P <0.05), and compared with the substrate B without the replanting flowers, the replanting flowers planted on the surface of the novel artificial substrate can obviously enhance the activity of the dehydrogenase, thereby macroscopically enhancing the self-cleaning capability of a river channel and improving the microenvironment of a water body.
TABLE 1A, B Total Difference assay for substrate biofilm dehydrogenase Activity
In addition, in order to prove the influence of seasons on the activity of the novel artificial matrix of the invention on the activity of the biofilm dehydrogenase, the sampling time in the step (1) of sample collection and treatment is respectively 5 months, 8 months, 11 months and the next 1 month, and the sample treatment and the method for measuring the activity of the biofilm dehydrogenase are not changed.
As shown in FIG. 2, A, B matrix biofilm dehydrogenase activity varied at the same level throughout the season. The differences of the activity of the biomembrane dehydrogenase in four seasons are analyzed, the analysis result is shown in table 2, the difference of the activity of the dehydrogenase on the surface of the novel artificial substrate (A substrate) between autumn and summer has statistical significance (P <0.05), and the activity of the dehydrogenase is generally shown to be higher in autumn than in summer.
TABLE 2 differential analysis of the dehydrogenase activity of the biofilm on the surface of the novel Artificial substrate (substrate A) in four seasons
Although particular embodiments of the invention have been described and illustrated in detail, it should be understood that various equivalent changes and modifications could be made to the above-described embodiments in accordance with the spirit of the invention, and the resulting functional effects would still fall within the scope of the invention.
Claims (5)
1. A method for activating the activity of a biological membrane enzyme by combining a novel artificial substrate and a plant, which is characterized in that: the method comprises the steps of placing a novel artificial substrate on the bank side, planting plants on the upper surface of the novel artificial substrate, and restoring the ecological system of the urban inland river channel through the interaction of plant roots and a biological membrane on the surface of the substrate.
2. The method of claim 1 for activating the enzyme activity of a biofilm by combining a novel artificial substrate with a plant, wherein the method comprises: the novel artificial substrate is a three-dimensional net structure formed by mutually lapping polyvinyl chloride or polypropylene through hot melting of disordered filaments and then carrying out extrusion molding through a die.
3. The method of claim 1 for activating the enzyme activity of a biofilm by combining a novel artificial substrate with a plant, wherein the method comprises: the plants planted on the upper surface of the novel artificial substrate are relish flowers.
4. The method of claim 3 for activating the enzyme activity of a biofilm by combining a novel artificial substrate with a plant, wherein the method comprises: the novel artificial substrate is placed on the bank of a river, the upper surface of the novel artificial substrate is flush with the water surface, and the second-forced flower young plants are planted on the upper surface of the novel artificial substrate and fixed by a small amount of soil.
5. A novel method of activating enzymatic activity of a biofilm by combining an artificial substrate with a plant according to any one of claims 1 to 4, wherein: the biomembrane enzyme is mainly derived from dehydrogenase.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104213537A (en) * | 2014-05-22 | 2014-12-17 | 温州医科大学 | Combined design of substrate applicable to slope ecological transformation and construction method of substrate |
| CN104211181A (en) * | 2014-08-28 | 2014-12-17 | 温州医科大学 | Matrix design and construction method for interception band of gutter inlet and drain outlet |
| CN107686167A (en) * | 2017-10-16 | 2018-02-13 | 浙江省冶金研究院有限公司 | A kind of drowned flow artificial wet land ecosystem and processing method for handling domestic sewage in rural areas |
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- 2019-12-13 CN CN201911282178.9A patent/CN110963581A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104213537A (en) * | 2014-05-22 | 2014-12-17 | 温州医科大学 | Combined design of substrate applicable to slope ecological transformation and construction method of substrate |
| CN104211181A (en) * | 2014-08-28 | 2014-12-17 | 温州医科大学 | Matrix design and construction method for interception band of gutter inlet and drain outlet |
| CN107686167A (en) * | 2017-10-16 | 2018-02-13 | 浙江省冶金研究院有限公司 | A kind of drowned flow artificial wet land ecosystem and processing method for handling domestic sewage in rural areas |
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Application publication date: 20200407 |