CN110951617A

CN110951617A - Solid culture method of EM (effective microorganisms)

Info

Publication number: CN110951617A
Application number: CN201911384991.7A
Authority: CN
Inventors: 赖国亮
Original assignee: Xiamen Yunchuang Biotechnology Co Ltd
Current assignee: Xiamen Yunchuang Biotechnology Co Ltd
Priority date: 2019-12-28
Filing date: 2019-12-28
Publication date: 2020-04-03

Abstract

The invention provides a solid culture method of EM bacteria, which comprises the steps of putting a solid culture medium containing rice bran, molasses and bean powder into a clean culture tank, sterilizing, inoculating the EM bacteria, and then carrying out fermentation culture. The method has low cost, and the number of effective viable bacteria cultured is as high as 20 hundred million/g.

Description

Solid culture method of EM (effective microorganisms)

Technical Field

The invention relates to a solid culture method of EM (effective microorganisms), belonging to the technical field of microorganisms.

Background

The EM bacteria, named as 'Effective microorganisms' in English, is a live bacteria preparation which is prepared by compositely culturing more than 10 microorganisms of more than 80 genera, such as photosynthetic bacteria, actinomycetes, saccharomycetes, compound lactic acid bacteria, bacillus and the like, wherein various microorganisms in the EM bacteria preparation are symbiotic with each other and interact with each other to jointly develop and exert various functions, and the action mechanism is to form competition of the EM bacteria and pathogenic microorganisms for nutrition and form advantageous communities of beneficial microorganisms, so that the propagation of the pathogenic microorganisms is controlled. The EM bacteria have extremely high application value in the industries of industry, agriculture and medicine, and are also commonly applied.

The culture of EM has a very wide application prospect, but the existing culture method of EM mainly adopts a liquid culture medium for culture, but the liquid culture medium needs to be artificially added with various nutrient substances for configuration according to the growth requirements of EM, the cost of various nutrient substances is high, and the nutrition cannot completely meet the growth requirements of EM. If the common crops such as soybean, grain and the like can be directly prepared into a solid culture medium for culture, the cost is greatly saved, and the nutrition is complete. However, no method for culturing EM bacteria by using the solid medium is developed in the prior art.

Disclosure of Invention

The invention provides a solid culture method of EM (effective microorganisms) bacteria, which can effectively solve the problems.

The invention is realized by the following steps:

a solid culture method of EM bacteria comprises adding solid culture medium containing testa oryzae, testa Tritici, molasses and semen glycines powder into cleaned culture tank, sterilizing, inoculating EM bacteria, and fermenting.

As a further improvement, the formula of the solid culture medium is as follows: 50-65 wt% of rice bran, 17-22 wt% of bran, 8-12 wt% of molasses and 16-22 wt% of bean flour.

As a further improvement, the inoculation amount of the EM bacteria is 1-10 wt%.

As a further improvement, the grain size of the rice bran is larger than 60 meshes, the grain size of the bran is larger than 60 meshes, and the sugar content of the molasses is not less than 45 wt%.

As a further improvement, the sterilization operation is: adjusting the water content of the solid culture medium to 50-55 wt%, and then keeping the solid culture medium at 100-120 ℃ for 1.5-2.5 h.

As a further improvement, the inoculation operation is that: and when the temperature of the solid culture medium is reduced to 30-35 ℃, adjusting the water content of the solid culture medium to 40-45 wt%, adding EM (effective microorganism) strains, and uniformly stirring.

As a further improvement, the temperature of the fermentation culture is 30-38 ℃, and the period of the fermentation culture is 6-8 days.

As a further improvement, the EM bacteria after fermentation culture also need to be dried and then sealed and packaged.

As a further improvement, the fermentation culture process needs to be stirred once every 1.5-2.5 hours.

As a further improvement, the drying is drying by sterile wind, and then airing until the moisture content is lower than 15 wt%.

The invention has the beneficial effects that:

the solid culture medium adopted by the solid culture method of EM bacteria of the invention contains rice bran, wheat bran, molasses and bean powder which are derived from natural substances, and the solid culture method is cheap and easy to obtain, has simple configuration and reduces the cost.

The solid culture medium adopted by the solid culture method of the EM bacteria has complete nutrition of rice bran, molasses and bean powder, the EM bacteria can be well cultured without adding other nutrient substances, and the number of the cultured effective viable bacteria is up to 20 hundred million/g through detection.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

FIG. 1 is a flow chart of the culture of EM bacteria according to the embodiment of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.

Example 1

Cleaning the residual materials in the culture tank with clear water, heating the culture tank to 90 deg.C, sterilizing for 30 min, and evaporating to dryness. Solid media, whose formulation is shown in table 1, was fed into the culture tank. The stirring was turned on. And adjusting the moisture content of the material to 55% according to the moisture content of the raw material detected by the quality control. And after the feeding is finished, the feeding port is sealed, the induced draft fan is closed, the emptying valve of the culture tank is opened, and the opening degree is adjusted to 1/3. Starting a hot water pump, heating and sterilizing the materials, starting timing when the temperature reaches 100 ℃, and stopping heating after sterilizing for 2 hours. And fully opening an exhaust valve and a sterile air inlet valve to start cooling. When the temperature of the material is reduced to 35 ℃, sampling and detecting the moisture of the material, and if the moisture content is more than 40%, adding no water; if the water content is less than 40%, adding water to adjust to 40%. Simultaneously adding 5kg of EM strain stock (purchased from Luoyang Ookuruke Biotechnology Co., Ltd.), continuously stirring for 2 hr, stirring the stock uniformly, stopping stirring, and starting fermentation culture. The continuous ventilation is kept in the culture process, and the opening degree of the air inlet valve and the air outlet valve is adjusted to ensure a certain positive pressure in the tank. The culture tank was stirred to set: every 2 hours, stirring was carried out for 30 minutes. The culture temperature is maintained between 30-38 ℃. And after 7 days of fermentation culture, sealing the feed inlet of the culture tank and closing the exhaust valve. And then starting stirring, starting a draught fan, closing a sterile air inlet valve, starting drying, stopping the draught fan within two hours, putting in a tank, and sampling and conveying samples. After the materials are put in the tank, the materials are aired according to the moisture condition of the materials, and when the moisture is lower than 15%, the materials are packaged.

The strain powder can be naturally fermented in an environment with certain humidity and moisture, so that the activity of microorganisms is influenced. Therefore, it is recommended to pay attention to humidity control during storage, select good envelope packaging and pay attention to humidity of storage environment. During storage, the interference and destruction of mice, ants and other animals are noticed. If the size of the finished product is not uniform, the finished product can be uniformly crushed by using a crusher.

TABLE 1 formulation of solid culture Medium

The detection is carried out according to the method of the national standard GB 20287 of the agricultural microbial inoculum, and the number of effective viable bacteria reaches 20 hundred million/g.

Example 2

The formula of the solid culture medium is changed into 300kg of rice bran, 395kg of bran, 200kg of molasses and 100kg of bean flour, and the rest is the same as that of the example 1. The detection shows that the effective viable count is 15.8 hundred million/gram.

Example 3

The formula of the solid culture medium is changed into 450kg of rice bran, 295kg of wheat bran, 200kg of molasses and 50kg of soybean flour, and the rest is the same as that of the example 1. The detection shows that the effective viable count is 12.5 hundred million/gram.

Example 4

The formula of the solid culture medium is changed into 350kg of rice bran, 295kg of wheat bran, 100kg of molasses and 150kg of bean flour, and the rest is the same as that of the example 1. The detection shows that the effective viable count is 13.2 hundred million/gram.

Example 5

The formula of the solid culture medium is changed into 450kg of rice bran, 50kg of wheat bran, 200kg of molasses and 295kg of soybean flour, and the rest is the same as that of the example 1. The detection shows that the effective viable count is 14.6 hundred million/gram.

Therefore, when the formula of the solid culture medium comprises 595kg of rice bran, 200kg of wheat bran, 200kg of molasses and 200kg of soybean flour, the formula has the highest number of effective viable bacteria and is the best formula.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A solid culture method of EM (effective microorganisms) is characterized by comprising the following steps: adding solid culture medium containing testa oryzae, testa Tritici, molasses and semen glycines powder into cleaned culture tank, sterilizing, inoculating EM bacteria, and fermenting.

2. The solid culture method of EM bacteria according to claim 1, wherein: the formula of the solid culture medium is as follows: 50-65 wt% of rice bran, 17-22 wt% of bran, 8-12 wt% of molasses and 16-22 wt% of bean flour.

3. The solid culture method of EM bacteria according to claim 1, wherein: the inoculation amount of the EM is 1-10 wt%.

4. The solid culture method of EM bacteria according to claim 2, wherein: the granularity of the rice bran is larger than 60 meshes, the granularity of the bran is larger than 60 meshes, and the sugar content of the molasses is not smaller than 45 wt%.

5. The solid culture method of EM bacteria according to claim 1, wherein: the sterilization operation is as follows: adjusting the water content of the solid culture medium to 50-55 wt%, and then keeping the solid culture medium at 100-120 ℃ for 1.5-2.5 h.

6. The solid culture method of EM bacteria according to claim 1, wherein: the inoculation operation comprises the following steps: and when the temperature of the solid culture medium is reduced to 30-35 ℃, adjusting the water content of the solid culture medium to 40-45 wt%, adding EM (effective microorganism) strains, and uniformly stirring.

7. The solid culture method of EM bacteria according to claim 1, wherein: the temperature of the fermentation culture is 30-38 ℃, and the period of the fermentation culture is 6-8 days.

8. The solid culture method of EM bacteria according to claim 1, wherein: and drying the EM bacteria after fermentation culture, and then sealing and packaging.

9. The solid culture method of EM bacteria according to claim 1, wherein: the fermentation culture process needs to be stirred once every 1.5-2.5 h.

10. The solid culture method of EM bacteria according to claim 8, wherein: the drying is carried out by drying with sterile wind and then airing until the water content is lower than 15 wt%.