CN110946288A - Novel antioxidant soybean peptide powder and preparation method thereof - Google Patents
Novel antioxidant soybean peptide powder and preparation method thereof Download PDFInfo
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- CN110946288A CN110946288A CN201911226267.1A CN201911226267A CN110946288A CN 110946288 A CN110946288 A CN 110946288A CN 201911226267 A CN201911226267 A CN 201911226267A CN 110946288 A CN110946288 A CN 110946288A
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 61
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- 235000010469 Glycine max Nutrition 0.000 title claims abstract description 60
- 239000003963 antioxidant agent Substances 0.000 title claims abstract description 27
- 230000003078 antioxidant effect Effects 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 26
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 40
- 108010073771 Soybean Proteins Proteins 0.000 claims abstract description 33
- 235000019710 soybean protein Nutrition 0.000 claims abstract description 33
- 239000004365 Protease Substances 0.000 claims abstract description 31
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- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 26
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- 229920001661 Chitosan Polymers 0.000 claims abstract description 20
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 20
- 229920002774 Maltodextrin Polymers 0.000 claims abstract description 20
- 239000005913 Maltodextrin Substances 0.000 claims abstract description 20
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 20
- 235000020661 alpha-linolenic acid Nutrition 0.000 claims abstract description 20
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 claims abstract description 20
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 claims abstract description 20
- 229960004488 linolenic acid Drugs 0.000 claims abstract description 20
- 229940035034 maltodextrin Drugs 0.000 claims abstract description 20
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- 238000006243 chemical reaction Methods 0.000 claims description 20
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- 108090000631 Trypsin Proteins 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 238000004925 denaturation Methods 0.000 claims description 5
- 230000036425 denaturation Effects 0.000 claims description 5
- 238000004945 emulsification Methods 0.000 claims description 5
- 230000002255 enzymatic effect Effects 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 5
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- 229940055729 papain Drugs 0.000 claims description 5
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- 208000007536 Thrombosis Diseases 0.000 description 1
- 229930003316 Vitamin D Natural products 0.000 description 1
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
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- 235000013402 health food Nutrition 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
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- 230000037356 lipid metabolism Effects 0.000 description 1
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- 238000012986 modification Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
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- 230000001766 physiological effect Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 239000000047 product Substances 0.000 description 1
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- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
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- 210000000813 small intestine Anatomy 0.000 description 1
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- 235000013619 trace mineral Nutrition 0.000 description 1
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- 239000011710 vitamin D Substances 0.000 description 1
- 150000003710 vitamin D derivatives Chemical class 0.000 description 1
- 229940046008 vitamin d Drugs 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
- A23L5/32—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation using phonon wave energy, e.g. sound or ultrasonic waves
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention provides novel antioxidant soybean peptide powder and a preparation method thereof, and relates to the technical field of preparation of soybean peptide powder. The novel antioxidant soybean peptide powder comprises the following components in parts by weight: 80-100 parts of soybean protein powder, 2-3 parts of chitosan, 1-2 parts of tea polyphenol, 1-3 parts of maltodextrin, 2-4 parts of linolenic acid and 2-3 parts of vitamin C. The soybean peptide powder is prepared by utilizing the soybean protein powder, the chitosan, the tea polyphenol, the maltodextrin, the linolenic acid and the vitamin C, the bitter taste of the soybean peptide powder is changed, the mouthfeel of the soybean peptide powder is improved when the soybean peptide powder is eaten, and the oxidation resistance of the soybean peptide powder and the oxidation resistance of an eater are obviously enhanced; by pretreating the protease, the activity of the protease is improved, the soybean protein powder can be subjected to enzymolysis more thoroughly, and the enzymolysis efficiency is improved.
Description
Technical Field
The invention relates to the technical field of preparation of soybean peptide powder, in particular to novel antioxidant soybean peptide powder and a preparation method thereof.
Background
The soybean peptide powder is composed of 2-6 amino acids, compared with the original protein and the amino acid preparation composed of the same, the soybean peptide powder is easier to be absorbed by human body, can directly enter small intestine to be absorbed without gastrointestinal digestion, and is not influenced by physical condition, the soybean peptide powder contains various bioactive peptides, and has various physiological activities, including antioxidation, blood pressure reduction, cholesterol reduction, blood fat reduction, fatigue resistance, immunity enhancement and the like; as a novel multifunctional nutritional ingredient, the soybean peptide powder can be widely used in health food, infant food, sports food, fermented products, clinical nutritional preparations and the like.
The soybean peptide powder is rich in nutrition and easy to absorb, and has the effects of resisting hypertension, reducing cholesterol, resisting thrombosis, improving lipid metabolism, preventing arteriosclerosis, enhancing physical strength and muscle strength of a human body, promoting recovery of myoerythrocytes, eliminating fatigue, promoting absorption of calcium, phosphorus and other trace elements, forming soybean peptide complex calcium, directly entering blood, depositing in bones, and achieving the effect of improving the strength of bones without participation of vitamin D.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides novel antioxidant soybean peptide powder and a preparation method thereof, and solves the defects and shortcomings of the prior art.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: the novel antioxidant soybean peptide powder comprises the following components in parts by weight: 80-100 parts of soybean protein powder, 2-3 parts of chitosan, 1-2 parts of tea polyphenol, 1-3 parts of maltodextrin, 2-4 parts of linolenic acid and 2-3 parts of vitamin C.
Preferably, the soybean peptide powder consists of the following components in parts by weight: 80 parts of soybean protein powder, 2 parts of chitosan, 1 part of tea polyphenol, 1 part of maltodextrin, 2 parts of linolenic acid and 2 parts of vitamin C.
Preferably, the soybean peptide powder consists of the following components in parts by weight: 90 parts of soybean protein powder, 2.5 parts of chitosan, 1.5 parts of tea polyphenol, 2 parts of maltodextrin, 3 parts of linolenic acid and 2.5 parts of vitamin C.
Preferably, the soybean peptide powder consists of the following components in parts by weight: 100 parts of soybean protein powder, 3 parts of chitosan, 2 parts of tea polyphenol, 3 parts of maltodextrin, 4 parts of linolenic acid and 3 parts of vitamin C.
A preparation method of novel antioxidant soybean peptide powder comprises the following preparation steps:
s1, mixing the soybean protein powder with a proper amount of water, fully and uniformly stirring the soybean protein powder and the water by using an emulsification pump, and then carrying out thermal denaturation on the obtained soybean protein solution to prepare a denatured protein solution;
s2, putting the denatured protein solution into a reaction tank, adding protease into the reaction tank, pretreating the protease in the tank, adjusting the temperature inside the reaction tank to be 40-50 ℃ and the pH value to be 6-9, and carrying out enzymolysis on the denatured protein solution in the reaction tank for 3-5 hours;
s3, after the enzymolysis is finished, carrying out enzyme deactivation treatment to obtain an enzymolysis liquid, then carrying out cooling treatment on the enzymolysis liquid, and after the enzymolysis liquid is cooled to room temperature, carrying out filtration treatment on the enzymolysis liquid by using an ultrafiltration membrane;
s4, adding chitosan, tea polyphenol, maltodextrin, linolenic acid and vitamin C into the filtered enzymatic hydrolysate, fully stirring the mixture, and homogenizing by using a homogenizer after stirring;
s5, carrying out vacuum concentration treatment on the homogenized mixed material, then carrying out sterilization treatment on the treated mixed material, and finally obtaining the soybean peptide powder by using a spray drying method.
Preferably, the protease pretreatment is ultrasonic pretreatment, the treatment time is 5-10min, and the power during ultrasonic treatment is 100-120W.
Preferably, the protease is one or more of neutral protease, trypsin, alkaline protease and papain.
Preferably, the temperature for enzyme deactivation is 70-90 ℃, and the treatment time is 10-60 s.
(III) advantageous effects
The invention provides novel antioxidant soybean peptide powder and a preparation method thereof. Comprises the following
Has the advantages that:
1. according to the novel antioxidant soybean peptide powder and the preparation method thereof, the soybean peptide powder is prepared by utilizing the soybean protein powder, the chitosan, the tea polyphenol, the maltodextrin, the linolenic acid and the vitamin C, the bitter taste of the soybean peptide powder is changed, the mouthfeel of the soybean peptide powder is improved when the soybean peptide powder is eaten, and the antioxidant performance of the soybean peptide powder and the antioxidant function of an eater are obviously enhanced.
2. According to the novel antioxidant soybean peptide powder and the preparation method thereof, the activity of protease is improved by pretreating the protease, so that the soybean protein powder can be subjected to enzymolysis more thoroughly, and meanwhile, the enzymolysis efficiency is also improved.
Detailed Description
The following will clearly and completely describe the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
the embodiment of the invention provides novel antioxidant soybean peptide powder which comprises the following components in parts by weight: 80 parts of soybean protein powder, 2 parts of chitosan, 1 part of tea polyphenol, 1 part of maltodextrin, 2 parts of linolenic acid and 2 parts of vitamin C.
A preparation method of novel antioxidant soybean peptide powder comprises the following preparation steps:
s1, mixing the soybean protein powder with a proper amount of water, fully and uniformly stirring the soybean protein powder and the water by using an emulsification pump, and then carrying out thermal denaturation on the obtained soybean protein solution to prepare a denatured protein solution;
s2, putting the denatured protein solution into a reaction tank, adding protease into the reaction tank, wherein the protease is one or more of neutral protease, trypsin, alkaline protease and papain, pretreating the protease in the tank, the protease pretreatment is ultrasonic pretreatment, the treatment time is 5-10min, the power is 100 and 120W during the ultrasonic treatment, then adjusting the temperature inside the reaction tank to be 40-50 ℃ and the PH value to be 6-9, and carrying out enzymolysis on the denatured protein solution in the reaction tank for 3-5 hours;
s3, after the enzymolysis is finished, carrying out enzyme deactivation treatment to obtain an enzymolysis liquid, wherein the temperature of the enzyme deactivation treatment is 70-90 ℃, the treatment time is 10-60S, then carrying out cooling treatment on the enzymolysis liquid, and filtering the enzymolysis liquid by using an ultrafiltration membrane after the enzymolysis liquid is cooled to room temperature;
s4, adding chitosan, tea polyphenol, maltodextrin, linolenic acid and vitamin C into the filtered enzymatic hydrolysate, fully stirring the mixture, and homogenizing by using a homogenizer after stirring;
s5, carrying out vacuum concentration treatment on the homogenized mixed material, then carrying out sterilization treatment on the treated mixed material, and finally obtaining the soybean peptide powder by using a spray drying method.
Example two:
the embodiment of the invention provides novel antioxidant soybean peptide powder which comprises the following components in parts by weight: 90 parts of soybean protein powder, 2.5 parts of chitosan, 1.5 parts of tea polyphenol, 2 parts of maltodextrin, 3 parts of linolenic acid and 2.5 parts of vitamin C.
A preparation method of novel antioxidant soybean peptide powder comprises the following preparation steps:
s1, mixing the soybean protein powder with a proper amount of water, fully and uniformly stirring the soybean protein powder and the water by using an emulsification pump, and then carrying out thermal denaturation on the obtained soybean protein solution to prepare a denatured protein solution;
s2, putting the denatured protein solution into a reaction tank, adding protease into the reaction tank, wherein the protease is one or more of neutral protease, trypsin, alkaline protease and papain, pretreating the protease in the tank, the protease pretreatment is ultrasonic pretreatment, the treatment time is 5-10min, the power is 100 and 120W during the ultrasonic treatment, then adjusting the temperature inside the reaction tank to be 40-50 ℃ and the PH value to be 6-9, and carrying out enzymolysis on the denatured protein solution in the reaction tank for 3-5 hours;
s3, after the enzymolysis is finished, carrying out enzyme deactivation treatment to obtain an enzymolysis liquid, wherein the temperature of the enzyme deactivation treatment is 70-90 ℃, the treatment time is 10-60S, then carrying out cooling treatment on the enzymolysis liquid, and filtering the enzymolysis liquid by using an ultrafiltration membrane after the enzymolysis liquid is cooled to room temperature;
s4, adding chitosan, tea polyphenol, maltodextrin, linolenic acid and vitamin C into the filtered enzymatic hydrolysate, fully stirring the mixture, and homogenizing by using a homogenizer after stirring;
s5, carrying out vacuum concentration treatment on the homogenized mixed material, then carrying out sterilization treatment on the treated mixed material, and finally obtaining the soybean peptide powder by using a spray drying method.
Example three:
the embodiment of the invention provides novel antioxidant soybean peptide powder which comprises the following components in parts by weight: 100 parts of soybean protein powder, 3 parts of chitosan, 2 parts of tea polyphenol, 3 parts of maltodextrin, 4 parts of linolenic acid and 3 parts of vitamin C.
A preparation method of novel antioxidant soybean peptide powder comprises the following preparation steps:
s1, mixing the soybean protein powder with a proper amount of water, fully and uniformly stirring the soybean protein powder and the water by using an emulsification pump, and then carrying out thermal denaturation on the obtained soybean protein solution to prepare a denatured protein solution;
s2, putting the denatured protein solution into a reaction tank, adding protease into the reaction tank, wherein the protease is one or more of neutral protease, trypsin, alkaline protease and papain, pretreating the protease in the tank, the protease pretreatment is ultrasonic pretreatment, the treatment time is 5-10min, the power is 100 and 120W during the ultrasonic treatment, then adjusting the temperature inside the reaction tank to be 40-50 ℃ and the PH value to be 6-9, and carrying out enzymolysis on the denatured protein solution in the reaction tank for 3-5 hours;
s3, after the enzymolysis is finished, carrying out enzyme deactivation treatment to obtain an enzymolysis liquid, wherein the temperature of the enzyme deactivation treatment is 70-90 ℃, the treatment time is 10-60S, then carrying out cooling treatment on the enzymolysis liquid, and filtering the enzymolysis liquid by using an ultrafiltration membrane after the enzymolysis liquid is cooled to room temperature;
s4, adding chitosan, tea polyphenol, maltodextrin, linolenic acid and vitamin C into the filtered enzymatic hydrolysate, fully stirring the mixture, and homogenizing by using a homogenizer after stirring;
s5, carrying out vacuum concentration treatment on the homogenized mixed material, then carrying out sterilization treatment on the treated mixed material, and finally obtaining the soybean peptide powder by using a spray drying method.
According to the invention, the soybean peptide powder is prepared by utilizing the soybean protein powder, the chitosan, the tea polyphenol, the maltodextrin, the linolenic acid and the vitamin C, so that the bitterness of the soybean peptide powder is changed, the mouthfeel of the soybean peptide powder is improved when the soybean peptide powder is eaten, and the oxidation resistance of the soybean peptide powder and the oxidation resistance of an eater are obviously enhanced; by pretreating the protease, the activity of the protease is improved, the soybean protein powder can be subjected to enzymolysis more thoroughly, and the enzymolysis efficiency is improved.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. A novel antioxidant soybean peptide powder is characterized in that: the soybean peptide powder comprises the following components in parts by weight: 80-100 parts of soybean protein powder, 2-3 parts of chitosan, 1-2 parts of tea polyphenol, 1-3 parts of maltodextrin, 2-4 parts of linolenic acid and 2-3 parts of vitamin C.
2. The novel antioxidant soybean peptide powder as claimed in claim 1, wherein: the soybean peptide powder comprises the following components in parts by weight: 80 parts of soybean protein powder, 2 parts of chitosan, 1 part of tea polyphenol, 1 part of maltodextrin, 2 parts of linolenic acid and 2 parts of vitamin C.
3. The novel antioxidant soybean peptide powder as claimed in claim 1, wherein: the soybean peptide powder comprises the following components in parts by weight: 90 parts of soybean protein powder, 2.5 parts of chitosan, 1.5 parts of tea polyphenol, 2 parts of maltodextrin, 3 parts of linolenic acid and 2.5 parts of vitamin C.
4. The novel antioxidant soybean peptide powder as claimed in claim 1, wherein: the soybean peptide powder comprises the following components in parts by weight: 100 parts of soybean protein powder, 3 parts of chitosan, 2 parts of tea polyphenol, 3 parts of maltodextrin, 4 parts of linolenic acid and 3 parts of vitamin C.
5. A preparation method of novel antioxidant soybean peptide powder is characterized by comprising the following steps: the preparation method comprises the following preparation steps:
s1, mixing the soybean protein powder with a proper amount of water, fully and uniformly stirring the soybean protein powder and the water by using an emulsification pump, and then carrying out thermal denaturation on the obtained soybean protein solution to prepare a denatured protein solution;
s2, putting the denatured protein solution into a reaction tank, adding protease into the reaction tank, pretreating the protease in the tank, adjusting the temperature inside the reaction tank to be 40-50 ℃ and the pH value to be 6-9, and carrying out enzymolysis on the denatured protein solution in the reaction tank for 3-5 hours;
s3, after the enzymolysis is finished, carrying out enzyme deactivation treatment to obtain an enzymolysis liquid, then carrying out cooling treatment on the enzymolysis liquid, and after the enzymolysis liquid is cooled to room temperature, carrying out filtration treatment on the enzymolysis liquid by using an ultrafiltration membrane;
s4, adding chitosan, tea polyphenol, maltodextrin, linolenic acid and vitamin C into the filtered enzymatic hydrolysate, fully stirring the mixture, and homogenizing by using a homogenizer after stirring;
s5, carrying out vacuum concentration treatment on the homogenized mixed material, then carrying out sterilization treatment on the treated mixed material, and finally obtaining the soybean peptide powder by using a spray drying method.
6. The novel antioxidant soybean peptide powder and the preparation method thereof as claimed in claim 5, wherein: the protease pretreatment is ultrasonic pretreatment, the treatment time is 5-10min, and the power is 100-120W during ultrasonic treatment.
7. The novel antioxidant soybean peptide powder and the preparation method thereof as claimed in claim 5, wherein: the protease is one or more of neutral protease, trypsin, alkaline protease and papain.
8. The novel antioxidant soybean peptide powder and the preparation method thereof as claimed in claim 5, wherein: the temperature for enzyme deactivation is 70-90 deg.C, and the treatment time is 10-60 s.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111616373A (en) * | 2020-07-02 | 2020-09-04 | 西咸新区优恩生物科技股份有限公司 | Aerospace civil nutrition formula suitable for people with cardio-cerebral regulation |
| CN114521629A (en) * | 2021-12-25 | 2022-05-24 | 北京天第食圣食品有限公司 | Raw wet noodles and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2013031453A (en) * | 2012-10-11 | 2013-02-14 | Rohto Pharmaceutical Co Ltd | Gelled food with soy peptide |
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| CN114521629A (en) * | 2021-12-25 | 2022-05-24 | 北京天第食圣食品有限公司 | Raw wet noodles and preparation method thereof |
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