CN110946212A

CN110946212A - Solid-state fermentation method and solid-state fermentation device for biological peptide for animal feed

Info

Publication number: CN110946212A
Application number: CN201911207820.7A
Authority: CN
Inventors: 麦露; 李永红
Original assignee: Qingyuan Bafu Biological Co ltd
Current assignee: Qingyuan Bafu Biological Co ltd
Priority date: 2019-11-30
Filing date: 2019-11-30
Publication date: 2020-04-03

Abstract

The invention discloses a solid-state fermentation method of biological peptide for animal feed, which comprises the steps of S1 raw material weighing; s2, mixing and stirring; s3 ultrasonic processing; s4 fermenting the raw material; the weight percentages are as follows: 15-25 parts of amino acid, 20-30 parts of agar, 15-25 parts of glucose, 10-20 parts of antioxidant, 2-4 parts of yeast extract, 10-20 parts of active protein, 1-3 parts of marine product, 5-10 parts of probiotic bacteria, 0.5-1 part of digestive enzyme and 0.5-1 part of polysaccharide. The invention also discloses a solid-state fermentation device of the biological peptide for the animal feed. Amino acid is adopted as raw material for fermentation, marine products provide organic trace elements, probiotics are beneficial to protecting intestinal health, digestive enzyme provides feed conversion rate, polysaccharide provides energy and growth factors for organisms quickly, ultrasonic waves are adopted for reaction, and the concentration of the biological peptide for the animal feed is more uniform during manufacturing.

Description

Solid-state fermentation method and solid-state fermentation device for biological peptide for animal feed

Technical Field

The invention relates to the technical field of pig feed fermentation production, in particular to a solid-state fermentation method and a solid-state fermentation device for biological peptide for animal feed.

Background

Pig feed is generally a feed for domestic pigs consisting of protein feed, energy feed, roughage, greenfeed, silage, mineral feed and feed additives; classifying according to categories: the complete feed is a mixed batch consisting of four parts, namely protein feed, energy feed, coarse feed and an additive, is mainly granulated feed processed and granulated by a machine, is partially expanded granular feed, can be directly used for feeding a feeding object, and can comprehensively meet the nutritional requirement of the feeding object; the traditional biological peptide solid state fermentation method for the animal feed is complex to operate, organic trace elements cannot be provided, the feed is not beneficial to protecting intestinal health, the feed conversion rate is low during fermentation, the concentration uniformity cannot be guaranteed during manufacturing, the pig feed fermentation cannot be facilitated, meanwhile, the traditional pig feed solid state fermentation device is of an integrated structure, the traditional pig feed solid state fermentation device cannot be freely installed and disassembled, the maintenance and cleaning of the inside of the solid state fermentation device by a user cannot be facilitated, meanwhile, a protection device is not provided, external impact easily causes the shaking, impacting and damaging of the internal elements of the solid state fermentation device, and the service life of the solid; in view of these drawbacks, it is necessary to design a solid-state fermentation method and a solid-state fermentation apparatus for a biological peptide for animal feed.

Disclosure of Invention

The present invention is directed to a solid-state fermentation method and a solid-state fermentation apparatus for biological peptides for animal feed, which solve the problems of the background art mentioned above.

In order to solve the technical problems, the invention provides the following technical scheme:

a solid-state fermentation method of biological peptide for animal feed is characterized by comprising the following steps:

s1: weighing raw materials, namely weighing the raw materials in parts by mass as follows: 15-25 parts of amino acid, 20-30 parts of agar, 15-25 parts of glucose, 10-20 parts of antioxidant, 2-4 parts of yeast extract, 10-20 parts of active protein, 1-3 parts of marine product, 5-10 parts of probiotic bacteria, 0.5-1 part of digestive enzyme and 0.5-1 part of polysaccharide;

s2: mixing and stirring:

1) cleaning a fermentation barrel, adding amino acid and agar, and slowly stirring for 1-2 h;

2) adding antioxidant, glucose and yeast extract, heating to 30-40 deg.C, and slowly stirring;

3) mashing marine products, stirring, adding into a fermentation barrel, adding active protein, keeping the temperature of the materials, and continuously stirring for 1-2 h;

s3: ultrasonic treatment

1) Placing the mixture in the fermentation barrel in an ultrasonic machine, and stirring for 10min manually in advance;

2) adding the stirred mixture into digestive enzyme, starting an ultrasonic machine, and continuously processing and reacting for 1-2 h;

3) taking out the processed mixture, pouring the mixture into a fermentation barrel and storing the mixture;

s4: fermentation of raw materials

1) Adding probiotics and polysaccharide into the mixture in the fermentation barrel, and manually stirring for 10 min;

2) placing the stirred mixture in a heat-insulating barrel, and covering a sealing cover;

3) standing for 5-10h to allow the mixture to perform self-fermentation reaction to obtain the solid-state fermented biological peptide.

The amino acid is one or more of lysine, tryptophan, leucine, threonine, desmosine, methionine, phenylalanine, arginine, histidine, aspartic acid, proline, serine, glycine, alanine and isoleucine.

The marine product is mixture of fish, shellfish, meat and plants.

A solid fermentation device for animal feed biological peptide for implementing the method comprises a fermentation container shell, a handle, a sealing cover, a base, a fixed rod, a turnover paddle, a fermentation container barrel, a supporting seat, a rotating shaft, a servo motor, a motor shell, a buffer cushion, a movable plate, a clamping groove, a first telescopic pipe, a second telescopic pipe, a clamping plate, a first spring plate, a spongy cushion, a compression spring, an anti-slip pad, a second spring plate, a threaded column, a threaded hole, a self-locking nut, a baffle, a rolling ball, a central hole, a rotating rod and a ball groove, wherein the sealing cover is arranged at the top of the fermentation container shell, the handle is welded at the top of the sealing cover, the fixed rod is welded at the center of the bottom of the sealing cover, the turnover paddle is welded at the bottom of the fixed rod, the servo motor is arranged at the center of the, the bottom of the motor shell is connected with the inner wall of the bottom of the fermentation container shell, a rotating shaft is arranged at the top of the servo motor, a supporting seat is welded at the top of the rotating shaft, a ball groove is formed in the top of the motor shell, a rotating rod is welded at the top of the inside of the ball groove, a rolling ball is sleeved on the outer side of the rotating rod and is positioned at the bottom of the supporting seat, a fermentation container barrel is arranged at the top of the supporting seat, turning blades are positioned inside the fermentation container barrel, clamping grooves are formed in two sides of the bottom of the fermentation container barrel, threaded columns are welded at two ends of the supporting seat, a movable plate is sleeved at one end of each threaded column, threaded holes are formed in the bottom of the movable plate and are sleeved outside the threaded columns, a self-locking nut is sleeved at one end of each threaded column and positioned, the clamp is characterized in that a plurality of second telescopic pipes are welded on the other side of the top of the movable plate, one end of each second telescopic pipe is sleeved with a first telescopic pipe, one end of each first telescopic pipe is welded with a clamp plate, the clamp plates are located inside the clamping grooves, a first spring plate is welded at the center of one end of each clamp plate, one end of each first spring plate is sleeved with a compression spring, one end of each compression spring is sleeved with a second spring plate, and the second spring plate is connected with one side of the movable plate.

A plurality of bases are fixed at the bottom of the fermentation container shell through screws, and cushion pads are bonded at the bottoms of the bases.

A sponge mat is bonded on one side of the clamping plate, a non-slip mat is bonded on one end of the sponge mat, and the non-slip mat is located inside the clamping groove.

The center of the rolling ball is provided with a center hole which is sleeved outside the rotating rod.

Compared with the prior art, the invention has the following beneficial effects:

1. the solid-state fermentation method of the biological peptide for the animal feed adopts amino acid as a raw material for fermentation, marine products provide organic trace elements, probiotics are beneficial to protecting intestinal health, digestive enzymes provide feed conversion rate, polysaccharide provides energy and growth factors for organisms quickly, and the biological peptide for the animal feed adopts ultrasonic waves for reaction during solid-state fermentation production, so that the concentration of the biological peptide for the animal feed is more uniform during production;

2. the solid fermentation device of the biological peptide for the animal feed is simple in structure and convenient to operate, the self-locking nut is screwed, the movable plate extrudes the compression spring to enable the clamping plate to clamp the fermentation container barrel to complete fixation, the traditional integrated structure is abandoned, the device can be freely installed and disassembled, maintenance and cleaning of the fermentation container barrel by a user are facilitated, meanwhile, the turnover blades are arranged in the fermentation container barrel, internal pig feed is stirred through rotation of the fermentation container barrel, stirring is more uniform, and processing and production of the pig feed are facilitated;

3. this solid fermentation device of biological peptide for animal feed simple structure, convenient operation, when external striking, the extrusion compression spring is rocked to the fermentation vessel shell, and compression spring provides elasticity buffering impact, protects the fermentation vessel bucket, avoids the fermentation vessel bucket because the striking rocks and the damage of fermentation vessel shell friction, is favorable to protecting the fermentation vessel bucket, prolongs this solid fermentation device life of biological peptide for animal feed.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:

FIG. 1 is a schematic process flow diagram of a solid state fermentation process of the present invention;

FIG. 2 is a schematic view of the overall structure of the solid fermentation apparatus of the present invention;

FIG. 3 is a sectional view showing the entire structure of the solid fermentation apparatus of the present invention;

FIG. 4 is an enlarged view of the movable plate of the solid fermentation apparatus of the present invention;

FIG. 5 is an enlarged view of the rolling bead of the solid fermentation apparatus of the present invention;

in the figure: 1. a fermentation vessel housing; 2. a handle; 3. sealing the cover; 4. a base; 5. fixing the rod; 6. turning over the paddle; 7. a fermenter vessel; 8. a supporting seat; 9. a rotating shaft; 10. a servo motor; 11. a motor housing; 12. a cushion pad; 13. a movable plate; 14. a card slot; 15. a first telescopic tube; 16. a second telescopic tube; 17. a splint; 18. a first spring plate; 19. a sponge cushion; 20. a compression spring; 21. a non-slip mat; 22. a second spring plate; 23. a threaded post; 24. a threaded hole; 25. a self-locking nut; 26. a baffle plate; 27. rolling the beads; 28. a central bore; 29. rotating the rod; 30. a ball groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1:

referring to fig. 1 to 5, a method for solid state fermentation of a bio-peptide for animal feed according to an embodiment of the present invention includes the following steps: s1, weighing the raw materials; s2, mixing and stirring; s3, ultrasonic treatment; s4, fermenting the raw materials; s5, bottling and storing;

weighing the following raw materials in parts by weight: 16 parts of amino acid, 25 parts of agar, 20 parts of glucose, 10 parts of antioxidant, 4 parts of yeast extract, 10 parts of active protein, 3 parts of marine product, 10 parts of probiotic bacteria, 1 part of digestive enzyme and 1 part of polysaccharide;

in S2, the mixing and stirring step includes the following steps:

in S3, the ultrasonic processing includes the following steps:

wherein in the above S4, the fermentation of the raw material comprises the steps of:

3) standing for 5-10h to allow the mixture to perform self-fermentation reaction to obtain solid-state fermented biological peptide;

in the step S5, the prepared solid-state fermented biological peptides are packaged in bottles, the same component is guaranteed for each bottle, the bottled solid-state fermented biological peptides are packaged in boxes and stored integrally, and the solid-state fermented biological peptides are stored in the shade.

Wherein the amino acid is one or more of lysine, tryptophan, leucine, threonine, desmosine, methionine, phenylalanine, arginine, histidine, aspartic acid, proline, serine, glycine, alanine and isoleucine; the marine product is mixture of fish, shellfish, meat and plant.

Referring to fig. 2-5, the solid fermentation apparatus for bio-peptide for animal feed provided by the embodiment of the present invention includes a fermentation container housing 1, a handle 2, a cover 3, a base 4, a fixing rod 5, a turning paddle 6, a fermentation container barrel 7, a supporting seat 8, a rotating shaft 9, a servo motor 10, a motor housing 11, a buffer pad 12, a movable plate 13, a slot 14, a first telescopic tube 15, a second telescopic tube 16, a clamping plate 17, a first spring plate 18, a sponge pad 19, a compression spring 20, an anti-slip pad 21, a second spring plate 22, a threaded post 23, a threaded hole 24, a self-locking nut 25, a baffle 26, a rolling ball 27, a central hole 28, a rotating rod 29 and a ball groove 30, wherein the base 4 is fixed at the bottom of the fermentation container housing 1 by screws, the buffer pad 12 is bonded at the bottom of the base 4, which is beneficial for fixing the fermentation container housing 1, the cover 3 is disposed, the top of the sealing cover 3 is welded with a handle 2, the center of the bottom of the sealing cover 3 is welded with a fixed rod 5, the bottom of the fixed rod 5 is welded with a turnover paddle 6, the center of the inner wall of the bottom of the fermentation container shell 1 is provided with a servo motor 10, both ends of the servo motor 10 are both welded with motor shells 11, the bottom of the motor shell 11 is connected with the inner wall of the bottom of the fermentation container shell 1, the top of the servo motor 10 is provided with a rotating shaft 9, the top of the rotating shaft 9 is welded with a supporting seat 8, the top of the motor shell 11 is provided with a ball groove 30, the top of the inside of the ball groove 30 is welded with a rotating rod 29, the outside of the rotating rod 29 is sleeved with a rolling ball 27, the rolling ball 27 is positioned at the bottom of the supporting seat 8, the center of the rolling ball 27 is provided with a, clamping grooves 14 are respectively formed in two sides of the bottom of a fermentation container barrel 7, threaded columns 23 are welded at two ends of a supporting seat 8, a movable plate 13 is sleeved at one end of each threaded column 23, a threaded hole 24 is formed in the bottom of the movable plate 13, the threaded hole 24 is sleeved on the outer side of the threaded column 23, a self-locking nut 25 is sleeved at one end of each threaded column 23, the self-locking nut 25 is located on one side of the movable plate 13, a baffle 26 is welded at one end of each threaded column 23, the baffle 26 is located on one side of the self-locking nut 25, a plurality of second telescopic pipes 16 are welded on the other side of the top of the movable plate 13, a first telescopic pipe 15 is sleeved at one end of each second telescopic pipe 16, a clamping plate 17 is welded at one end of each first telescopic pipe 15, the clamping plate 17 is located inside the clamping groove 14, a sponge cushion 19 is bonded at one side of each clamping plate, one end of the first spring plate 18 is sleeved with a compression spring 20, one end of the compression spring 20 is sleeved with a second spring plate 22, and the second spring plate 22 is connected with one side of the movable plate 13.

Based on the above, the invention has the advantages that when the device is used, the solid-state fermented biological peptide pig feed is manually placed in the fermentation container barrel 7, the fermentation container barrel 7 is clamped between the movable plates 13, the self-locking nut 25 is rotated clockwise to extrude the movable plates 13, the movable plates 13 extrude the compression springs 20, the compression springs 20 provide elastic force to extrude the clamping plates 17, the clamping plates 17 clamp the fermentation container barrel 7 to complete fixation, the sealing cover 3 is covered on the top of the fermentation container shell 1, the servo motor 10 is started, the servo motor 10 rotates to drive the fermentation container barrel 7 to rotate, so that the solid-state fermented biological peptide pig feed in the fermentation container barrel 7 centrifugally rotates, and meanwhile, the pig feed is processed by turning over the paddle 6 to stir.

Example 2: the solid-state fermentation method and the solid-state fermentation device for the biological peptide for the animal feed provided by the embodiment of the invention are basically the same as the embodiment 1, and the difference is that:

s1: weighing raw materials, namely weighing the raw materials in parts by mass as follows: 15 parts of amino acid, 20 parts of agar, 15 parts of glucose, 10 parts of antioxidant, 2 parts of yeast extract, 10 parts of active protein, 1 part of marine product, 5 parts of probiotic bacteria, 0.5 part of digestive enzyme and 0.5 part of polysaccharide;

example 3: the solid-state fermentation method and the solid-state fermentation device for the biological peptide for the animal feed provided by the embodiment of the invention are basically the same as the

embodiments

1 and 2, and the difference is that:

s1: weighing raw materials, namely weighing the raw materials in parts by mass as follows: 25 parts of amino acid, 30 parts of agar, 25 parts of glucose, 20 parts of antioxidant, 4 parts of yeast extract, 20 parts of active protein, 2.5 parts of marine products, 7.5 parts of probiotics, 0.8 part of digestive enzyme and 0.8 part of polysaccharide.

In the above examples, amino acids and marine products can be selected from any of the above descriptions, and the technical effects described in the present invention can be achieved, so they are not listed.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A solid-state fermentation method of biological peptide for animal feed is characterized by comprising the following steps:

s2: mixing and stirring:

s3: ultrasonic treatment

s4: fermentation of raw materials

2. The solid-state fermentation method of a bio-peptide for animal feed according to claim 1, characterized in that: the amino acid is one or more of lysine, tryptophan, leucine, threonine, desmosine, methionine, phenylalanine, arginine, histidine, aspartic acid, proline, serine, glycine, alanine and isoleucine.

3. The solid-state fermentation method of a bio-peptide for animal feed according to claim 1, characterized in that: the marine product is mixture of fish, shellfish, meat and plants.

4. A solid fermentation device of biological peptide for animal feed for implementing the method of any one of claims 1 to 3, comprising a fermentation container housing (1), a handle (2), a cover (3), a base (4), a fixing rod (5), a turnover paddle (6), a fermentation container barrel (7), a supporting seat (8), a rotating shaft (9), a servo motor (10), a motor housing (11), a buffer cushion (12), a movable plate (13), a clamping groove (14), a first telescopic pipe (15), a second telescopic pipe (16), a clamping plate (17), a first spring plate (18), a sponge pad (19), a compression spring (20), an anti-skid pad (21), a second spring plate (22), a threaded column (23), a threaded hole (24), a self-locking nut (25), a baffle plate (26), a rolling ball (27), a central hole (28), a rotating rod (29) and a ball groove (30), the method is characterized in that: the fermentation container shell (1) top is provided with a sealing cover (3), the top of the sealing cover (3) is welded with a handle (2), the center of the bottom of the sealing cover (3) is welded with a fixing rod (5), the bottom of the fixing rod (5) is welded with a turnover paddle (6), the center of the inner wall of the bottom of the fermentation container shell (1) is provided with a servo motor (10), the two ends of the servo motor (10) are both welded with a motor shell (11), the bottom of the motor shell (11) is connected with the inner wall of the bottom of the fermentation container shell (1), the top of the servo motor (10) is provided with a rotating shaft (9), the top of the rotating shaft (9) is welded with a supporting seat (8), the top of the motor shell (11) is provided with a ball groove (30), the top of the ball groove (30) is welded with a rotating rod (29), and, the rolling ball (27) is positioned at the bottom of the supporting seat (8), a fermentation container barrel (7) is arranged at the top of the supporting seat (8), the turnover paddle (6) is positioned inside the fermentation container barrel (7), clamping grooves (14) are formed in two sides of the bottom of the fermentation container barrel (7), threaded columns (23) are welded at two ends of the supporting seat (8), a movable plate (13) is sleeved at one end of each threaded column (23), a threaded hole (24) is formed in the bottom of the movable plate (13), the threaded hole (24) is sleeved on the outer side of the threaded column (23), a self-locking nut (25) is sleeved at one end of each threaded column (23), the self-locking nut (25) is positioned on one side of the movable plate (13), a baffle (26) is welded at one end of each threaded column (23), the baffle (26) is positioned on one side of the self-locking nut (25), and a plurality of second telescopic, first flexible pipe (15) has been cup jointed to second flexible pipe (16) one end, splint (17) have been welded to first flexible pipe (15) one end, and splint (17) are located inside draw-in groove (14), splint (17) one end center department welds first spring board (18), compression spring (20) have been cup jointed to first spring board (18) one end, compression spring (20) one end has been cup jointed second spring board (22), and second spring board (22) are connected with fly leaf (13) one side.

5. The apparatus for solid fermentation of a bio-peptide for animal feed according to claim 4, wherein: a plurality of bases (4) are fixed at the bottom of the fermentation container shell (1) through screws, and cushion pads (12) are bonded at the bottoms of the bases (4).

6. The apparatus for solid fermentation of a bio-peptide for animal feed according to claim 4, wherein: a spongy cushion (19) is bonded on one side of the clamping plate (17), an anti-slip pad (21) is bonded on one end of the spongy cushion (19), and the anti-slip pad (21) is located inside the clamping groove (14).

7. The apparatus for solid fermentation of a bio-peptide for animal feed according to claim 4, wherein: the center of the rolling ball (27) is provided with a center hole (28), and the center hole (28) is sleeved on the outer side of the rotating rod (29).