CN110946100B - Application of sea cucumber oligopeptide in inducing attachment of mytilus coruscus young shellfish - Google Patents
Application of sea cucumber oligopeptide in inducing attachment of mytilus coruscus young shellfish Download PDFInfo
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- CN110946100B CN110946100B CN201911334722.XA CN201911334722A CN110946100B CN 110946100 B CN110946100 B CN 110946100B CN 201911334722 A CN201911334722 A CN 201911334722A CN 110946100 B CN110946100 B CN 110946100B
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- sea cucumber
- cucumber oligopeptide
- inducing
- mytilus coruscus
- oligopeptide
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- 241000251511 Holothuroidea Species 0.000 title claims abstract description 50
- 108010038807 Oligopeptides Proteins 0.000 title claims abstract description 45
- 102000015636 Oligopeptides Human genes 0.000 title claims abstract description 45
- 241000143233 Mytilus coruscus Species 0.000 title claims abstract description 37
- 230000001939 inductive effect Effects 0.000 title claims abstract description 25
- 235000015170 shellfish Nutrition 0.000 title description 4
- 230000000366 juvenile effect Effects 0.000 claims abstract description 31
- 241000237852 Mollusca Species 0.000 claims abstract description 21
- 239000012528 membrane Substances 0.000 claims abstract description 8
- 239000000843 powder Substances 0.000 claims abstract description 8
- 239000013535 sea water Substances 0.000 claims abstract description 5
- 238000000909 electrodialysis Methods 0.000 claims abstract description 4
- 238000000926 separation method Methods 0.000 claims abstract description 4
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 238000001694 spray drying Methods 0.000 claims description 3
- 230000009849 deactivation Effects 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 6
- 241000237525 Mytilidae Species 0.000 description 3
- 241000237536 Mytilus edulis Species 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 235000020638 mussel Nutrition 0.000 description 3
- 241000237519 Bivalvia Species 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000863430 Shewanella Species 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000009193 crawling Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention provides application of sea cucumber oligopeptide in inducing juvenile mytilus coruscus, which is characterized in that the sea cucumber oligopeptide is subjected to enzymolysis, membrane separation, electrodialysis and membrane concentration to obtain sea cucumber oligopeptide powder, and the sea cucumber oligopeptide powder is dissolved in sterilized seawater to obtain a sea cucumber oligopeptide solution; the sea cucumber oligopeptide has the activity of inducing the adhesion of mytilus coruscus juvenile mollusks, the sea cucumber oligopeptide solutions with different concentrations have different inducing activities on the juvenile mollusks, when the initial concentration is 0.5mg/mL, the sea cucumber oligopeptide has the highest inducing rate on the juvenile mollusks, and when the concentration of the sea cucumber oligopeptide is higher than 0.5mg/mL, the inducing rate on the juvenile mollusks is obviously reduced, so the sea cucumber oligopeptide has the effect of inducing the adhesion of the mytilus coruscus juvenile mollusks, and the optimal addition concentration is 0.5 mg/mL; therefore, the invention provides theoretical basis for improving the attachment quality of the mytilus coruscus and artificial culture of the mytilus coruscus.
Description
Technical Field
The invention belongs to the technical field of induction of juvenile mytilus coruscus, and particularly relates to application of sea cucumber oligopeptide in induction of juvenile mytilus coruscus.
Background
Mytilus coruscus (A) and (B)Mytilus coruscus）Belongs to the phylum Mollusca (Mollusca), Bivalvia (Bivalvia), Mytilidae (Mytilodia) and Mytilidae (Mytilidae), is an important marine shellfish culture variety in China and is distributed along the coast of the yellow sea, the Bohai sea and the east sea. The mytilus coruscus has high nutritive value and wide cultivation prospectAnd market development potential. In the life history of mytilus coruscus, eyespot larvae can be attached and transformed into juvenile mollusks and finally develop into adult mollusks at the later stage of the planktonic life stage. In the process of growing juvenile mussels into adult mussels, when the environment changes, juvenile mussels of Mytilus coruscus can automatically cut byssus, start crawling and reselect a proper adhesive base for re-adhesion. Mytilus coruscus is an important marine shellfish culture variety, and the fingerlings are mainly obtained by natural fingerlings and semi-artificial fingerlings. In recent years, due to over-development of fishery resources, natural seedlings cannot meet the development of the mytilus coruscus breeding industry. Therefore, it is necessary to solve the problem of germchit and to explore bioengineering techniques such as inducing the adhesion substance of Mytilus coruscus.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide the application of sea cucumber oligopeptide in inducing the attachment of mytilus coruscus juvenile mollusks.
In order to achieve the above purpose, the solution of the invention is as follows:
an application of sea cucumber oligopeptide in inducing the attachment of young mytilus coruscus.
Furthermore, the sea cucumber oligopeptide is dried and crushed sea cucumber.
Further, the sea cucumber is subjected to enzymolysis, enzyme deactivation, membrane separation, electrodialysis, membrane concentration and spray drying to obtain sea cucumber oligopeptide powder, and the sea cucumber oligopeptide powder is dissolved in sterilized seawater to obtain a sea cucumber oligopeptide solution.
Wherein the initial concentration of the sea cucumber oligopeptide solution is 0.1mg/mL, 0.3mg/mL, 0.5mg/mL, 1.0mg/mL and 2.0mg/mL respectively.
Due to the adoption of the scheme, the invention has the beneficial effects that:
the sea cucumber oligopeptide has the activity of inducing the attachment of the mytilus coruscus juvenile mollusks, the sea cucumber oligopeptide solutions with different concentrations have different inducing activities on the juvenile mollusks, the sea cucumber oligopeptide has the highest inducing rate on the juvenile mollusks when the initial concentration is 0.5mg/mL, and the inducing rate on the juvenile mollusks is obviously reduced when the concentration of the sea cucumber oligopeptide is higher than 0.5mg/mL, so that the optimal addition concentration of the sea cucumber oligopeptide is 0.5 mg/mL. Therefore, the invention provides theoretical basis for improving the attachment quality of the mytilus coruscus and artificial culture of the mytilus coruscus.
Drawings
FIG. 1 is a schematic diagram of the inducing effect of sea cucumber oligopeptide with different initial concentrations on the adhesion of mytilus coruscus juvenile (the ordinate represents the adhesion rate, and the abscissa represents the Concentration). Note: different letters represent significant differences (P＜0.05）。
Detailed Description
The invention provides sea cucumber oligopeptide and application thereof in inducing juvenile mytilus coruscus to attach.
The sea cucumber oligopeptide is dissolved in seawater and directly acts on juvenile mytilus coruscus, and the action effect is more direct. The marine Shewanella bacteria induce the adhesion of Mytilus coruscus juvenile mollusks by forming a microbial capsule, so the substances for inducing Mytilus coruscus juvenile mollusks are different, and the action principle is also different.
The sea cucumber oligopeptide is from Dalian blue peptide research and development Limited company. The specific production process comprises the following steps: taking dried and crushed sea cucumber, adding pure water according to the feed-liquid ratio of 1:10 (g/mL), adding compound protease under the conditions of 48 ℃ and pH value of 8.5, stirring and carrying out enzymolysis for 4h, inactivating enzyme, carrying out membrane separation, electrodialysis, membrane concentration and spray drying to obtain sea cucumber oligopeptide powder with the molecular weight of below 1000 Da.
The sea cucumber oligopeptide disclosed by the invention is used for inducing the adhesion of young mytilus coruscus as follows. The main steps are that sea cucumber oligopeptide powder is weighed and dissolved in sterilized sea water, and the sea cucumber oligopeptide solution is prepared after full stirring, so that the initial concentration is 0.1mg/mL, 0.3mg/mL, 0.5mg/mL, 1.0mg/mL and 2.0mg/mL respectively. A sterilized glass slide is placed in each culture dish, the sea cucumber oligopeptide solution and 10 juvenile mytilus coruscus (the shell length is 0.71 +/-0.05 mm, the shell height is 0.43 +/-0.06 mm) are added into the culture dish, and the attachment rate of the juvenile mytilus coruscus is recorded at 48h, namely the number of the juvenile mytilus coruscus attached to the glass slide accounts for the total number of the juvenile mytilus coruscus in the culture dish.
Researches show that the sea cucumber oligopeptide has the activity of inducing the adhesion of mytilus coruscus juvenile mollusks, as shown in fig. 1, the inducing activity of the sea cucumber oligopeptide solutions with different concentrations on the juvenile mollusks is different, when the initial concentration is 0.5mg/mL, the inducing rate of the sea cucumber oligopeptide on the juvenile mollusks is the highest, the adhesion rate is improved by 66.7% compared with that of a blank control group, and when the concentration of the sea cucumber oligopeptide is higher than 0.5mg/mL, the inducing rate on the juvenile mollusks is obviously reduced. Therefore, the sea cucumber oligopeptide has the effect of inducing the adhesion of the juvenile mytilus coruscus, and the optimal addition concentration is 0.5 mg/mL. Therefore, the method provides theoretical basis for improving the attachment quality of the mytilus coruscus and artificial culture of the mytilus coruscus.
The foregoing description of the specific embodiments is presented to enable any person skilled in the art to make and use the invention. It will be readily apparent to those skilled in the art that various modifications can be made and the generic principles described herein can be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above-described embodiments. Those skilled in the art should appreciate that many modifications and variations are possible in light of the above teaching without departing from the scope of the invention.
Claims (1)
1. Application of sea cucumber oligopeptide in inducing attachment of mytilus coruscus juvenile mollusk;
the sea cucumber oligopeptide is dried and crushed sea cucumber;
performing enzymolysis, enzyme deactivation, membrane separation, electrodialysis, membrane concentration and spray drying on the sea cucumber to obtain sea cucumber oligopeptide powder;
dissolving the sea cucumber oligopeptide powder in sterilized seawater to obtain a sea cucumber oligopeptide solution;
the initial concentration of the sea cucumber oligopeptide solution is 0.5 mg/mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911334722.XA CN110946100B (en) | 2019-12-23 | 2019-12-23 | Application of sea cucumber oligopeptide in inducing attachment of mytilus coruscus young shellfish |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911334722.XA CN110946100B (en) | 2019-12-23 | 2019-12-23 | Application of sea cucumber oligopeptide in inducing attachment of mytilus coruscus young shellfish |
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| Publication Number | Publication Date |
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| CN110946100A CN110946100A (en) | 2020-04-03 |
| CN110946100B true CN110946100B (en) | 2021-11-30 |
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| CN201911334722.XA Active CN110946100B (en) | 2019-12-23 | 2019-12-23 | Application of sea cucumber oligopeptide in inducing attachment of mytilus coruscus young shellfish |
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| CN1182761A (en) * | 1997-10-05 | 1998-05-27 | 中国科学院海洋研究所 | Polypeptide paint for preventing pollution by sea living things and preparing method and usage thereof |
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| WO2014020129A2 (en) * | 2012-08-01 | 2014-02-06 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Allatostatin-b peptides for inducing the settlement of lophotrochozoan marine larvae |
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| CN105132498A (en) * | 2015-07-30 | 2015-12-09 | 北京天肽生物科技有限公司 | Micromolecular sea cucumber peptide and preparation method thereof |
| CN109007649A (en) * | 2018-06-29 | 2018-12-18 | 烟台源力德海洋生物有限公司 | A kind of preparation method and applications of sea cucumber oyster glycopeptide calcium complexing compound |
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Inventor after: Liang Xiao Inventor after: Xu Jiakang Inventor after: Peng Lihua Inventor after: Yang Jinlong Inventor after: Bao Weiyang Inventor before: Liang Xiao Inventor before: Xu Jiakang Inventor before: Peng Lihua Inventor before: Yang Jinlong Inventor before: Bao Weiyang |
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